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Q8TEW6 (DOK4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Docking protein 4
Alternative name(s):
Downstream of tyrosine kinase 4
Insulin receptor substrate 5
Short name=IRS-5
Short name=IRS5
Gene names
Name:DOK4
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length326 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK4 functions in RET-mediated neurite outgrowth and plays a positive role in activation of the MAP kinase pathway By similarity. Putative link with downstream effectors of RET in neuronal differentiation. May be involved in the regulation of the immune response induced by T-cells.

Subunit structure

Interacts with RET and TEK/TIE2. Interaction with RET is mediated through the PTB domain and requires phosphorylation of RET 'Tyr-1062' By similarity.

Tissue specificity

Widely expressed. High expression in skeletal muscle, heart, kidney and liver. Weaker expression in spleen, lung and small intestine, brain, heart and. Expressed in both resting and activated peripheral blood T-cells. Ref.1 Ref.2

Domain

PTB domain mediates receptor interaction By similarity.

Post-translational modification

Phosphorylated on tyrosine residues in response to insulin, IGF1 or RET stimulation. Ref.2

Sequence similarities

Belongs to the DOK family. Type B subfamily.

Contains 1 IRS-type PTB domain.

Contains 1 PH domain.

Sequence caution

The sequence AAC24310.1 differs from that shown. Reason: Erroneous gene model prediction.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 326326Docking protein 4
PRO_0000187274

Regions

Domain7 – 112106PH
Domain132 – 237106IRS-type PTB
Motif265 – 27511DKFBH motif

Experimental info

Sequence conflict641N → S no nucleotide entry Ref.2
Sequence conflict641N → S in BAA91642. Ref.3
Sequence conflict751T → A in AAL74195. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q8TEW6 [UniParc].

Last modified May 10, 2005. Version 2.
Checksum: 1595B0CEC5584816

FASTA32637,028
        10         20         30         40         50         60 
MATNFSDIVK QGYVKMKSRK LGIYRRCWLV FRKSSSKGPQ RLEKYPDEKS VCLRGCPKVT 

        70         80         90        100        110        120 
EISNVKCVTR LPKETKRQAV AIIFTDDSAR TFTCDSELEA EEWYKTLSVE CLGSRLNDIS 

       130        140        150        160        170        180 
LGEPDLLAPG VQCEQTDRFN VFLLPCPNLD VYGECKLQIT HENIYLWDIH NPRVKLVSWP 

       190        200        210        220        230        240 
LCSLRRYGRD ATRFTFEAGR MCDAGEGLYT FQTQEGEQIY QRVHSATLAI AEQHKRVLLE 

       250        260        270        280        290        300 
MEKNVRLLNK GTEHYSYPCT PTTMLPRSAY WHHITGSQNI AEASSYAGEG YGAAQASSET 

       310        320 
DLLNRFILLK PKPSQGDSSE AKTPSQ 

« Hide

References

« Hide 'large scale' references
[1]"DOK4 and DOK5: new Dok-related genes expressed in human T cells."
Favre C., Gerard A., Clauzier E., Pontarotti P., Olive D., Nunes J.A.
Genes Immun. 4:40-45(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Tissue: T-cell.
[2]"Two new substrates in insulin signaling, IRS5/DOK4 and IRS6/DOK5."
Cai D., Dhe-Paganon S., Melendez P.A., Lee J., Shoelson S.E.
J. Biol. Chem. 278:25323-25330(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, PHOSPHORYLATION AT TYROSINE RESIDUES.
Tissue: Skeletal muscle.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"Genome duplications and other features in 12 Mb of DNA sequence from human chromosome 16p and 16q."
Loftus B.J., Kim U.-J., Sneddon V.P., Kalush F., Brandon R., Fuhrmann J., Mason T., Crosby M.L., Barnstead M., Cronin L., Mays A.D., Cao Y., Xu R.X., Kang H.-L., Mitchell S., Eichler E.E., Harris P.C., Venter J.C., Adams M.D.
Genomics 60:295-308(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Colon and Pancreas.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF466369 mRNA. Translation: AAL74195.1.
AK001350 mRNA. Translation: BAA91642.1.
AC004382 Genomic DNA. Translation: AAC24310.1. Sequence problems.
BC001540 mRNA. Translation: AAH01540.1.
BC003541 mRNA. Translation: AAH03541.1.
RefSeqNP_060580.2. NM_018110.3.
XP_005256100.1. XM_005256043.1.
UniGeneHs.279832.

3D structure databases

ProteinModelPortalQ8TEW6.
SMRQ8TEW6. Positions 6-235.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120837. 5 interactions.
IntActQ8TEW6. 3 interactions.
MINTMINT-1491631.
STRING9606.ENSP00000344277.

PTM databases

PhosphoSiteQ8TEW6.

Polymorphism databases

DMDM84029600.

Proteomic databases

PaxDbQ8TEW6.
PRIDEQ8TEW6.

Protocols and materials databases

DNASU55715.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000340099; ENSP00000344277; ENSG00000125170.
ENST00000569548; ENSP00000455128; ENSG00000125170.
GeneID55715.
KEGGhsa:55715.
UCSCuc002elv.4. human.

Organism-specific databases

CTD55715.
GeneCardsGC16M057505.
HGNCHGNC:19868. DOK4.
HPAHPA052844.
HPA055849.
MIM608333. gene.
neXtProtNX_Q8TEW6.
PharmGKBPA134892921.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG256436.
HOGENOMHOG000247025.
HOVERGENHBG002356.
InParanoidQ8TEW6.
PhylomeDBQ8TEW6.
TreeFamTF324994.

Enzyme and pathway databases

SignaLinkQ8TEW6.

Gene expression databases

ArrayExpressQ8TEW6.
BgeeQ8TEW6.
CleanExHS_DOK4.
GenevestigatorQ8TEW6.

Family and domain databases

Gene3D2.30.29.30. 2 hits.
InterProIPR002404. Insln_rcpt_S1.
IPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
[Graphical view]
PfamPF02174. IRS. 1 hit.
PF00169. PH. 1 hit.
[Graphical view]
SMARTSM00233. PH. 1 hit.
SM00310. PTBI. 1 hit.
[Graphical view]
PROSITEPS51064. IRS_PTB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiDOK4.
GenomeRNAi55715.
NextBio60599.
PROQ8TEW6.
SOURCESearch...

Entry information

Entry nameDOK4_HUMAN
AccessionPrimary (citable) accession number: Q8TEW6
Secondary accession number(s): O75209, Q9BTP2, Q9NVV3
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 10, 2005
Last modified: March 19, 2014
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 16

Human chromosome 16: entries, gene names and cross-references to MIM