Reviewed,
UniProtKB/Swiss-Prot Q8TEW0 (PARD3_HUMAN)
Last modified
September 2, 2008.
Version 68.
History...
Clusters with 100%,
90%,
50% identity |
Documents (6) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Partitioning-defective 3 homolog Short name=PARD-3 Short name=PAR-3 Alternative name(s): Atypical PKC isotype-specific-interacting protein Short name=ASIP CTCL tumor antigen se2-5 PAR3-alpha | ||||
| Gene names |
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| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 1356 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. |
| Subunit structure | Interacts with PARD6A and PARD6B. Isoform 2, but not at least isoform 3 interacts with PRKCZ. Interacts with PRCKI By similarity. Part of a complex with PARD6A or PARD6B, PRKCI or PRKCZ and CDC42 or RAC1. Interacts with F11R/JAM1 By similarity. Component of a complex whose core is composed of ARHGAP17, AMOT, MPP5/PALS1, INADL/PATJ and PARD3/PAR3. Interacts with LIMK2. |
| Subcellular location | Intracytoplasmic membrane. Cell junction › tight junction. Cell membrane. Note= Partially localized along the cell-cell contact region. Colocalizes with PARD6A and PRKCI at epithelial tight junctions. |
| Tissue specificity | Widely expressed. |
| Post-translational modification | Phosphorylated by PRKCZ. EGF-induced Tyr-1127 phosphorylation mediates dissociation from LIMK2. |
| Miscellaneous | Antibodies against PARD3 are present in sera from patients with cutaneous T-cell lymphomas. |
| Sequence similarities | Belongs to the PAR3 family. Contains 3 PDZ (DHR) domains. |
| Sequence caution | The sequence AAG33676.1 differs from that shown. Reason: Miscellaneous discrepancy. Contaminating sequence. Potential poly-A sequence. |
Ontologies
Keywords | |
|---|---|
| Biological process | Cell cycle Cell division |
| Cellular component | Cell junction Cell membrane Membrane Tight junction |
| Coding sequence diversity | Alternative splicing Polymorphism |
| Domain | Coiled coil Repeat |
| PTM | Phosphoprotein |
Gene Ontology (GO) | |
| Biological process | activation of protein kinase C activity Traceable author statement. Source: UniProtKB asymmetric cell division Ref.1Traceable author statement. Source: ProtInc axonogenesisTraceable author statement. Source: UniProtKB establishment or maintenance of cell polarity Ref.1Traceable author statement. Source: UniProtKB protein complex assembly Ref.1Traceable author statement. Source: ProtInc |
| Cellular component | tight junction Ref.1 Inferred from direct assay. Source: UniProtKB |
| Molecular function | protein binding Ref.1 Inferred from sequence or structural similarity. Source: UniProtKB |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| PARD6A | Q9NPB6 | 2 | EBI-81968,EBI-81876 | |
| PARD6B | Q9BYG5 | 1 | EBI-81968,EBI-295391 | |
| Pard6b | Q9JK83 | 2 | EBI-81968,EBI-81861 | From a different organism. |
| PNMA1 | Q8ND90 | 1 | EBI-81968,EBI-302345 | |
| PRKCI | P41743 | 1 | EBI-81968,EBI-286199 | |
| YWHAB | P31946 | 1 | EBI-81968,EBI-359815 | |
| YWHAG | P61981 | 1 | EBI-81968,EBI-359832 | |
| YWHAH | Q04917 | 1 | EBI-81968,EBI-306940 | |
| YWHAZ | P63104 | 1 | EBI-81968,EBI-347088 |
Alternative products
| This entry describes 10 isoforms produced by alternative splicing. [Align] [Select] | |||||
| Isoform 1 (identifier: Q8TEW0-1) Also known as: A; This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | |||||
| Isoform 2 (identifier: Q8TEW0-2) Also known as: B; La; The sequence of this isoform differs from the canonical sequence as follows: 740-742: Missing. | |||||
| Isoform 3 (identifier: Q8TEW0-3) Also known as: C; The sequence of this isoform differs from the canonical sequence as follows: 195-238: Missing. 557-569: Missing. 740-742: Missing. 827-856: Missing. | |||||
| Isoform 4 (identifier: Q8TEW0-4) Also known as: D; The sequence of this isoform differs from the canonical sequence as follows: 1025-1061: Missing. | |||||
| Isoform 5 (identifier: Q8TEW0-5) Also known as: E; The sequence of this isoform differs from the canonical sequence as follows: 195-238: Missing. 557-569: Missing. 740-742: Missing. 857-857: I → S 858-872: Missing. 1025-1061: Missing. | |||||
| Isoform 6 (identifier: Q8TEW0-6) Also known as: F; The sequence of this isoform differs from the canonical sequence as follows: 557-569: Missing. 740-742: Missing. 827-856: Missing. | |||||
| Isoform 7 (identifier: Q8TEW0-7) Also known as: Lb; The sequence of this isoform differs from the canonical sequence as follows: 557-569: Missing. 740-742: Missing. 827-856: Missing. 1025-1061: Missing. | |||||
| Isoform 8 (identifier: Q8TEW0-8) Also known as: Sa; The sequence of this isoform differs from the canonical sequence as follows: 740-742: Missing. 1025-1034: RFGKHRKDDK → SLAKLKPEKR 1035-1356: Missing. | |||||
| Isoform 9 (identifier: Q8TEW0-9) Also known as: Sb; The sequence of this isoform differs from the canonical sequence as follows: 557-569: Missing. 740-742: Missing. 827-856: Missing. 1025-1034: RFGKHRKDDK → SLAKLKPEKR 1035-1356: Missing. | |||||
| Isoform 10 (identifier: Q8TEW0-10) The sequence of this isoform differs from the canonical sequence as follows: 740-742: Missing. 827-856: Missing. 857-858: IA → T 1025-1034: RFGKHRKDDK → SLAKLKPEKR 1035-1356: Missing. | |||||
| Notes: No experimental confirmation available. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | ||||
Molecule processing | ||||||||
|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1356 | 1356 | Partitioning-defective 3 homolog | |||||
Regions | ||||||||
| Domain | 271 – 359 | 89 | PDZ 1 | |||||
| Domain | 461 – 546 | 86 | PDZ 2 | |||||
| Domain | 590 – 677 | 88 | PDZ 3 | |||||
| Region | 712 – 936 | 225 | Interacts with PRKCZ By similarity | |||||
| Coiled coil | 1049 – 1077 | 29 | Potential | |||||
| Coiled coil | 1151 – 1174 | 24 | Potential | |||||
| Coiled coil | 1201 – 1224 | 24 | Potential | |||||
| Coiled coil | 1280 – 1301 | 22 | Potential | |||||
| Compositional bias | 984 – 1042 | 59 | Lys-rich | |||||
Amino acid modifications | ||||||||
| Modified residue | 143 | 1 | Phosphoserine | |||||
| Modified residue | 144 | 1 | Phosphoserine | |||||
| Modified residue | 383 | 1 | Phosphoserine | |||||
| Modified residue | 489 | 1 | Phosphotyrosine | |||||
| Modified residue | 695 | 1 | Phosphoserine | |||||
| Modified residue | 715 | 1 | Phosphoserine | |||||
| Modified residue | 717 | 1 | Phosphoserine | |||||
| Modified residue | 728 | 1 | Phosphoserine | |||||
| Modified residue | 827 | 1 | Phosphoserine By similarity | |||||
| Modified residue | 852 | 1 | Phosphoserine | |||||
| Modified residue | 1080 | 1 | Phosphotyrosine By similarity | |||||
| Modified residue | 1127 | 1 | Phosphotyrosine | |||||
Natural variations | ||||||||
| Alternative sequence | 195 – 238 | 44 | Missing in isoform 3 and isoform 5. | |||||
| Alternative sequence | 557 – 569 | 13 | Missing in isoform 3, isoform 5, isoform 6, isoform 7 and isoform 9. | |||||
| Alternative sequence | 740 – 742 | 3 | Missing in isoform 2, isoform 3, isoform 5, isoform 6, isoform 7, isoform 8, isoform 9 and isoform 10. | |||||
| Alternative sequence | 827 – 856 | 30 | Missing in isoform 3, isoform 6, isoform 7, isoform 9 and isoform 10. | |||||
| Alternative sequence | 857 – 858 | 2 | IA → T in isoform 10. | |||||
| Alternative sequence | 857 | 1 | I → S in isoform 5. | |||||
| Alternative sequence | 858 – 872 | 15 | Missing in isoform 5. | |||||
| Alternative sequence | 1025 – 1061 | 37 | Missing in isoform 4, isoform 5 and isoform 7. | |||||
| Alternative sequence | 1025 – 1034 | 10 | RFGKHRKDDK → SLAKLKPEKR in isoform 8, isoform 9 and isoform 10. | |||||
| Alternative sequence | 1035 – 1356 | 322 | Missing in isoform 8, isoform 9 and isoform 10. | |||||
| Natural variant | 107 | 1 | E → D: dbSNP rs1436731. | |||||
Experimental info | ||||||||
| Mutagenesis | 1127 | 1 | Y → F: Delayed epithelial tight junction assembly | |||||
| Sequence conflict | 190 | 1 | T → A in BAB55330. Ref.5 | |||||
| Sequence conflict | 233 | 1 | L → Q in BAB55330. Ref.5 | |||||
| Sequence conflict | 594 | 1 | N → S in BAB55330. Ref.5 | |||||
| Sequence conflict | 996 – 1000 | 5 | GKEKK → VELHE in BAA91366. Ref.5 | |||||
Sequences
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Clusters with