WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2 precursor

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Q8TEU8 (WFKN2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 88. History...

Clusters with 100%, 90%, 50% identity |

Documents (5) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2

Alternative name(s):
Growth and differentiation factor-associated serum protein 1
Short name=GASP-1
Short name=hGASP-1
WAP, follistatin, immunoglobulin, Kunitz and NTR domain-containing-related protein
WFIKKN-related protein

Gene names

Name:	WFIKKN2
Synonyms:	GASP1, WFIKKNRP
ORF Names:	UNQ9235/PRO31996

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	576 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Protease-inhibitor that contains multiple distinct protease inhibitor domains. Probably has serine protease- and metalloprotease-inhibitor activity. Inhibits the biological activity of mature myostatin, but not activin By similarity.
Subunit structure	Interacts with both mature and propeptide myostatin/MSTN. Ref.3
Subcellular location	Secreted By similarity.
Tissue specificity	Primarily expressed in ovary, testis and brain, but not in liver. In fetal tissues, it is primarily expressed in brain, skeletal muscle, thymus and kidney. Ref.1
Sequence similarities	Belongs to the WFIKKN family. Contains 2 BPTI/Kunitz inhibitor domains. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Kazal-like domain. Contains 1 NTR domain. Contains 1 WAP domain.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Immunoglobulin domain Repeat Signal
Molecular function	Metalloenzyme inhibitor Metalloprotease inhibitor Protease inhibitor Serine protease inhibitor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	transforming growth factor beta receptor signaling pathway Inferred from electronic annotation. Source: Compara
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metalloendopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 34

By similarity

Chain

35 – 576

542

WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2

PRO_0000307820

Regions

Domain

39 – 92

WAP

Domain

126 – 177

Kazal-like

Domain

210 – 303

Ig-like C2-type

Domain

328 – 378

BPTI/Kunitz inhibitor 1

Domain

386 – 436

BPTI/Kunitz inhibitor 2

Domain

445 – 566

122

NTR

Sites

Site

140 – 141

Reactive bond By similarity

Amino acid modifications

Glycosylation

319

N-linked (GlcNAc...) Potential

Glycosylation

519

N-linked (GlcNAc...) Potential

Disulfide bond

46 ↔ 79

By similarity

Disulfide bond

62 ↔ 83

By similarity

Disulfide bond

66 ↔ 78

By similarity

Disulfide bond

72 ↔ 88

By similarity

Disulfide bond

134 ↔ 164

By similarity

Disulfide bond

138 ↔ 157

By similarity

Disulfide bond

146 ↔ 175

By similarity

Disulfide bond

231 ↔ 287

By similarity

Disulfide bond

328 ↔ 378

By similarity

Disulfide bond

337 ↔ 361

By similarity

Disulfide bond

353 ↔ 374

By similarity

Disulfide bond

386 ↔ 436

By similarity

Disulfide bond

395 ↔ 419

By similarity

Disulfide bond

411 ↔ 432

By similarity

Disulfide bond

445 ↔ 515

By similarity

Disulfide bond

448 ↔ 517

By similarity

Disulfide bond

459 ↔ 566

By similarity

Natural variations

Natural variant

R → Q. Ref.2
Corresponds to variant rs55700534 [ dbSNP | Ensembl ].

VAR_061984

Natural variant

V → M.
Corresponds to variant rs35300894 [ dbSNP | Ensembl ].

VAR_036692

Sequences

Sequence

Length

Mass (Da)

Tools

Q8TEU8 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 08B4F2EDBE121F81
Show »

FASTA

576

63,941

        10         20         30         40         50         60 
MWAPRCRRFW SRWEQVAALL LLLLLLGVPP RSLALPPIRY SHAGICPNDM NPNLWVDAQS 

        70         80         90        100        110        120 
TCRRECETDQ ECETYEKCCP NVCGTKSCVA ARYMDVKGKK GPVGMPKEAT CDHFMCLQQG 

       130        140        150        160        170        180 
SECDIWDGQP VCKCKDRCEK EPSFTCASDG LTYYNRCYMD AEACSKGITL AVVTCRYHFT 

       190        200        210        220        230        240 
WPNTSPPPPE TTMHPTTASP ETPELDMAAP ALLNNPVHQS VTMGETVSFL CDVVGRPRPE 

       250        260        270        280        290        300 
ITWEKQLEDR ENVVMRPNHV RGNVVVTNIA QLVIYNAQLQ DAGIYTCTAR NVAGVLRADF 

       310        320        330        340        350        360 
PLSVVRGHQA AATSESSPNG TAFPAAECLK PPDSEDCGEE QTRWHFDAQA NNCLTFTFGH 

       370        380        390        400        410        420 
CHRNLNHFET YEACMLACMS GPLAACSLPA LQGPCKAYAP RWAYNSQTGQ CQSFVYGGCE 

       430        440        450        460        470        480 
GNGNNFESRE ACEESCPFPR GNQRCRACKP RQKLVTSFCR SDFVILGRVS ELTEEPDSGR 

       490        500        510        520        530        540 
ALVTVDEVLK DEKMGLKFLG QEPLEVTLLH VDWACPCPNV TVSEMPLIIM GEVDGGMAML 

       550        560        570 
RPDSFVGASS ARRVRKLREV MHKKTCDVLK EFLGLH

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Distinct expression pattern of two related human proteins containing multiple types of protease-inhibitory modules." Trexler M., Banyai L., Patthy L. Biol. Chem. 383:223-228(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLN-31.
[3]	"Regulation of myostatin in vivo by growth and differentiation factor-associated serum protein-1: a novel protein with protease inhibitor and follistatin domains." Hill J.J., Qiu Y., Hewick R.M., Wolfman N.M. Mol. Endocrinol. 17:1144-1154(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH MSTN.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF468657 mRNA. Translation: AAL77058.1. AY358142 mRNA. Translation: AAQ88509.1.
IPI	IPI00152847.
RefSeq	NP_783165.1. NM_175575.5.
UniGene	Hs.211475.
3D structure databases
HSSP	HSSP built from PDB template 1K09 based on UniProtKB P00974.
ProteinModelPortal	Q8TEU8.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8TEU8. 2 interactions.
STRING	9606.ENSP00000311184.
Protein family/group databases
MEROPS	I02.954.
PTM databases
PhosphoSite	Q8TEU8.
Polymorphism databases
DMDM	74716081.
Proteomic databases
PaxDb	Q8TEU8.
PRIDE	Q8TEU8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000311378; ENSP00000311184; ENSG00000173714.
GeneID	124857.
KEGG	hsa:124857.
UCSC	uc002isv.4. human.
Organism-specific databases
CTD	124857.
GeneCards	GC17P048912.
HGNC	HGNC:30916. WFIKKN2.
HPA	CAB025621. HPA010953. HPA011561.
MIM	610895. gene.
neXtProt	NX_Q8TEU8.
PharmGKB	PA134864812.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG261628.
HOGENOM	HOG000154855.
HOVERGEN	HBG108676.
InParanoid	Q8TEU8.
OMA	AQSTCKR.
OrthoDB	EOG4MSCXV.
PhylomeDB	Q8TEU8.
Gene expression databases
ArrayExpress	Q8TEU8.
Bgee	Q8TEU8.
CleanEx	HS_WFIKKN2.
Genevestigator	Q8TEU8.
Family and domain databases
Gene3D	2.60.40.10. 1 hit. 4.10.410.10. 2 hits. 4.10.75.10. 1 hit.
InterPro	IPR007110. Ig-like_dom. IPR013783. Ig-like_fold. IPR013098. Ig_I-set. IPR003598. Ig_sub2. IPR002350. Kazal_dom. IPR001134. Netrin_domain. IPR018933. Netrin_module_non-TIMP. IPR002223. Prot_inh_Kunz-m. IPR020901. Prtase_inh_Kunz-CS. IPR008993. TIMP-like_OB-fold. IPR008197. WAP-type_4-diS_core. [Graphical view]
Pfam	PF07679. I-set. 1 hit. PF07648. Kazal_2. 1 hit. PF00014. Kunitz_BPTI. 2 hits. PF01759. NTR. 1 hit. PF00095. WAP. 1 hit. [Graphical view]
PRINTS	PR00759. BASICPTASE.
SMART	SM00408. IGc2. 1 hit. SM00280. KAZAL. 1 hit. SM00131. KU. 2 hits. SM00217. WAP. 1 hit. [Graphical view]
SUPFAM	SSF57362. Prot_inh_Kunz-m. 2 hits. SSF50242. TIMP_like. 1 hit. SSF57256. WAP. 1 hit.
PROSITE	PS00280. BPTI_KUNITZ_1. 1 hit. PS50279. BPTI_KUNITZ_2. 2 hits. PS50835. IG_LIKE. 1 hit. PS00282. KAZAL_1. False negative. PS51465. KAZAL_2. 1 hit. PS50189. NTR. 1 hit. PS51390. WAP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	124857.
NextBio	81402.
SOURCE	Search...

Entry information

Entry name

WFKN2_HUMAN

Accession

Primary (citable) accession number: Q8TEU8
Secondary accession number(s): Q6UXZ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 2007
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Polymorphism databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents