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Q8TEU8

- WFKN2_HUMAN

UniProt

Q8TEU8 - WFKN2_HUMAN

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Protein

WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2

Gene

WFIKKN2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Protease-inhibitor that contains multiple distinct protease inhibitor domains. Probably has serine protease- and metalloprotease-inhibitor activity. Inhibits the biological activity of mature myostatin, but not activin (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei140 – 1412Reactive bondPROSITE-ProRule annotation

GO - Molecular functioni

  1. metalloendopeptidase inhibitor activity Source: UniProtKB-KW
  2. serine-type endopeptidase inhibitor activity Source: UniProtKB-KW

GO - Biological processi

  1. muscle fiber development Source: Ensembl
  2. negative regulation of DNA binding Source: Ensembl
  3. negative regulation of protein binding Source: Ensembl
  4. palate development Source: Ensembl
  5. skeletal system development Source: Ensembl
  6. transforming growth factor beta receptor signaling pathway Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Metalloenzyme inhibitor, Metalloprotease inhibitor, Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI02.954.

Names & Taxonomyi

Protein namesi
Recommended name:
WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2
Alternative name(s):
Growth and differentiation factor-associated serum protein 1
Short name:
GASP-1
Short name:
hGASP-1
WAP, follistatin, immunoglobulin, Kunitz and NTR domain-containing-related protein
WFIKKN-related protein
Gene namesi
Name:WFIKKN2
Synonyms:GASP1, WFIKKNRP
ORF Names:UNQ9235/PRO31996
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:30916. WFIKKN2.

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134864812.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3434By similarityAdd
BLAST
Chaini35 – 576542WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2PRO_0000307820Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi46 ↔ 79By similarity
Disulfide bondi62 ↔ 83By similarity
Disulfide bondi66 ↔ 78By similarity
Disulfide bondi72 ↔ 88By similarity
Disulfide bondi134 ↔ 164By similarity
Disulfide bondi138 ↔ 157By similarity
Disulfide bondi146 ↔ 175By similarity
Disulfide bondi231 ↔ 287By similarity
Glycosylationi319 – 3191N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi328 ↔ 378By similarity
Disulfide bondi337 ↔ 361By similarity
Disulfide bondi353 ↔ 374By similarity
Disulfide bondi386 ↔ 436By similarity
Disulfide bondi395 ↔ 419By similarity
Disulfide bondi411 ↔ 432By similarity
Disulfide bondi445 ↔ 515By similarity
Disulfide bondi448 ↔ 517By similarity
Disulfide bondi459 ↔ 566By similarity
Glycosylationi519 – 5191N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8TEU8.
PRIDEiQ8TEU8.

PTM databases

PhosphoSiteiQ8TEU8.

Expressioni

Tissue specificityi

Primarily expressed in ovary, testis and brain, but not in liver. In fetal tissues, it is primarily expressed in brain, skeletal muscle, thymus and kidney.1 Publication

Gene expression databases

BgeeiQ8TEU8.
CleanExiHS_WFIKKN2.
ExpressionAtlasiQ8TEU8. baseline.
GenevestigatoriQ8TEU8.

Organism-specific databases

HPAiCAB025621.
HPA010953.
HPA011561.

Interactioni

Subunit structurei

Interacts with both mature and propeptide myostatin/MSTN.1 Publication

Protein-protein interaction databases

IntActiQ8TEU8. 7 interactions.
MINTiMINT-8044275.
STRINGi9606.ENSP00000311184.

Structurei

3D structure databases

ProteinModelPortaliQ8TEU8.
SMRiQ8TEU8. Positions 137-173, 216-294, 337-436.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 9254WAPPROSITE-ProRule annotationAdd
BLAST
Domaini126 – 17752Kazal-likePROSITE-ProRule annotationAdd
BLAST
Domaini210 – 30394Ig-like C2-typeAdd
BLAST
Domaini328 – 37851BPTI/Kunitz inhibitor 1PROSITE-ProRule annotationAdd
BLAST
Domaini386 – 43651BPTI/Kunitz inhibitor 2PROSITE-ProRule annotationAdd
BLAST
Domaini445 – 566122NTRPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the WFIKKN family.Curated
Contains 2 BPTI/Kunitz inhibitor domains.PROSITE-ProRule annotation
Contains 1 Kazal-like domain.PROSITE-ProRule annotation
Contains 1 NTR domain.PROSITE-ProRule annotation
Contains 1 WAP domain.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal

Phylogenomic databases

eggNOGiNOG261628.
GeneTreeiENSGT00740000114929.
HOGENOMiHOG000154855.
HOVERGENiHBG108676.
InParanoidiQ8TEU8.
OMAiCPNVCGT.
OrthoDBiEOG754HSR.
PhylomeDBiQ8TEU8.
TreeFamiTF315349.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
4.10.410.10. 2 hits.
4.10.75.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003598. Ig_sub2.
IPR002350. Kazal_dom.
IPR001134. Netrin_domain.
IPR018933. Netrin_module_non-TIMP.
IPR002223. Prot_inh_Kunz-m.
IPR020901. Prtase_inh_Kunz-CS.
IPR008993. TIMP-like_OB-fold.
IPR008197. WAP.
[Graphical view]
PfamiPF07679. I-set. 1 hit.
PF07648. Kazal_2. 1 hit.
PF00014. Kunitz_BPTI. 2 hits.
PF01759. NTR. 1 hit.
PF00095. WAP. 1 hit.
[Graphical view]
PRINTSiPR00759. BASICPTASE.
SMARTiSM00408. IGc2. 1 hit.
SM00280. KAZAL. 1 hit.
SM00131. KU. 2 hits.
SM00217. WAP. 1 hit.
[Graphical view]
SUPFAMiSSF50242. SSF50242. 1 hit.
SSF57256. SSF57256. 1 hit.
SSF57362. SSF57362. 2 hits.
PROSITEiPS00280. BPTI_KUNITZ_1. 1 hit.
PS50279. BPTI_KUNITZ_2. 2 hits.
PS50835. IG_LIKE. 1 hit.
PS51465. KAZAL_2. 1 hit.
PS50189. NTR. 1 hit.
PS51390. WAP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8TEU8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MWAPRCRRFW SRWEQVAALL LLLLLLGVPP RSLALPPIRY SHAGICPNDM
60 70 80 90 100
NPNLWVDAQS TCRRECETDQ ECETYEKCCP NVCGTKSCVA ARYMDVKGKK
110 120 130 140 150
GPVGMPKEAT CDHFMCLQQG SECDIWDGQP VCKCKDRCEK EPSFTCASDG
160 170 180 190 200
LTYYNRCYMD AEACSKGITL AVVTCRYHFT WPNTSPPPPE TTMHPTTASP
210 220 230 240 250
ETPELDMAAP ALLNNPVHQS VTMGETVSFL CDVVGRPRPE ITWEKQLEDR
260 270 280 290 300
ENVVMRPNHV RGNVVVTNIA QLVIYNAQLQ DAGIYTCTAR NVAGVLRADF
310 320 330 340 350
PLSVVRGHQA AATSESSPNG TAFPAAECLK PPDSEDCGEE QTRWHFDAQA
360 370 380 390 400
NNCLTFTFGH CHRNLNHFET YEACMLACMS GPLAACSLPA LQGPCKAYAP
410 420 430 440 450
RWAYNSQTGQ CQSFVYGGCE GNGNNFESRE ACEESCPFPR GNQRCRACKP
460 470 480 490 500
RQKLVTSFCR SDFVILGRVS ELTEEPDSGR ALVTVDEVLK DEKMGLKFLG
510 520 530 540 550
QEPLEVTLLH VDWACPCPNV TVSEMPLIIM GEVDGGMAML RPDSFVGASS
560 570
ARRVRKLREV MHKKTCDVLK EFLGLH
Length:576
Mass (Da):63,941
Last modified:June 1, 2002 - v1
Checksum:i08B4F2EDBE121F81
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti31 – 311R → Q.1 Publication
Corresponds to variant rs55700534 [ dbSNP | Ensembl ].
VAR_061984
Natural varianti96 – 961V → M.
Corresponds to variant rs35300894 [ dbSNP | Ensembl ].
VAR_036692

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF468657 mRNA. Translation: AAL77058.1.
AY358142 mRNA. Translation: AAQ88509.1.
CCDSiCCDS11575.1.
RefSeqiNP_783165.1. NM_175575.5.
UniGeneiHs.211475.

Genome annotation databases

EnsembliENST00000311378; ENSP00000311184; ENSG00000173714.
GeneIDi124857.
KEGGihsa:124857.
UCSCiuc002isv.4. human.

Polymorphism databases

DMDMi74716081.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF468657 mRNA. Translation: AAL77058.1 .
AY358142 mRNA. Translation: AAQ88509.1 .
CCDSi CCDS11575.1.
RefSeqi NP_783165.1. NM_175575.5.
UniGenei Hs.211475.

3D structure databases

ProteinModelPortali Q8TEU8.
SMRi Q8TEU8. Positions 137-173, 216-294, 337-436.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q8TEU8. 7 interactions.
MINTi MINT-8044275.
STRINGi 9606.ENSP00000311184.

Protein family/group databases

MEROPSi I02.954.

PTM databases

PhosphoSitei Q8TEU8.

Polymorphism databases

DMDMi 74716081.

Proteomic databases

PaxDbi Q8TEU8.
PRIDEi Q8TEU8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000311378 ; ENSP00000311184 ; ENSG00000173714 .
GeneIDi 124857.
KEGGi hsa:124857.
UCSCi uc002isv.4. human.

Organism-specific databases

CTDi 124857.
GeneCardsi GC17P048912.
HGNCi HGNC:30916. WFIKKN2.
HPAi CAB025621.
HPA010953.
HPA011561.
MIMi 610895. gene.
neXtProti NX_Q8TEU8.
PharmGKBi PA134864812.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG261628.
GeneTreei ENSGT00740000114929.
HOGENOMi HOG000154855.
HOVERGENi HBG108676.
InParanoidi Q8TEU8.
OMAi CPNVCGT.
OrthoDBi EOG754HSR.
PhylomeDBi Q8TEU8.
TreeFami TF315349.

Miscellaneous databases

GenomeRNAii 124857.
NextBioi 81402.
PROi Q8TEU8.
SOURCEi Search...

Gene expression databases

Bgeei Q8TEU8.
CleanExi HS_WFIKKN2.
ExpressionAtlasi Q8TEU8. baseline.
Genevestigatori Q8TEU8.

Family and domain databases

Gene3Di 2.60.40.10. 1 hit.
4.10.410.10. 2 hits.
4.10.75.10. 1 hit.
InterProi IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003598. Ig_sub2.
IPR002350. Kazal_dom.
IPR001134. Netrin_domain.
IPR018933. Netrin_module_non-TIMP.
IPR002223. Prot_inh_Kunz-m.
IPR020901. Prtase_inh_Kunz-CS.
IPR008993. TIMP-like_OB-fold.
IPR008197. WAP.
[Graphical view ]
Pfami PF07679. I-set. 1 hit.
PF07648. Kazal_2. 1 hit.
PF00014. Kunitz_BPTI. 2 hits.
PF01759. NTR. 1 hit.
PF00095. WAP. 1 hit.
[Graphical view ]
PRINTSi PR00759. BASICPTASE.
SMARTi SM00408. IGc2. 1 hit.
SM00280. KAZAL. 1 hit.
SM00131. KU. 2 hits.
SM00217. WAP. 1 hit.
[Graphical view ]
SUPFAMi SSF50242. SSF50242. 1 hit.
SSF57256. SSF57256. 1 hit.
SSF57362. SSF57362. 2 hits.
PROSITEi PS00280. BPTI_KUNITZ_1. 1 hit.
PS50279. BPTI_KUNITZ_2. 2 hits.
PS50835. IG_LIKE. 1 hit.
PS51465. KAZAL_2. 1 hit.
PS50189. NTR. 1 hit.
PS51390. WAP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Distinct expression pattern of two related human proteins containing multiple types of protease-inhibitory modules."
    Trexler M., Banyai L., Patthy L.
    Biol. Chem. 383:223-228(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLN-31.
  3. "Regulation of myostatin in vivo by growth and differentiation factor-associated serum protein-1: a novel protein with protease inhibitor and follistatin domains."
    Hill J.J., Qiu Y., Hewick R.M., Wolfman N.M.
    Mol. Endocrinol. 17:1144-1154(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH MSTN.

Entry informationi

Entry nameiWFKN2_HUMAN
AccessioniPrimary (citable) accession number: Q8TEU8
Secondary accession number(s): Q6UXZ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: June 1, 2002
Last modified: October 29, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3