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Q8TEU8 (WFKN2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2
Alternative name(s):
Growth and differentiation factor-associated serum protein 1
Short name=GASP-1
Short name=hGASP-1
WAP, follistatin, immunoglobulin, Kunitz and NTR domain-containing-related protein
WFIKKN-related protein
Gene names
Name:WFIKKN2
Synonyms:GASP1, WFIKKNRP
ORF Names:UNQ9235/PRO31996
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length576 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Protease-inhibitor that contains multiple distinct protease inhibitor domains. Probably has serine protease- and metalloprotease-inhibitor activity. Inhibits the biological activity of mature myostatin, but not activin By similarity.

Subunit structure

Interacts with both mature and propeptide myostatin/MSTN. Ref.3

Subcellular location

Secreted By similarity.

Tissue specificity

Primarily expressed in ovary, testis and brain, but not in liver. In fetal tissues, it is primarily expressed in brain, skeletal muscle, thymus and kidney. Ref.1

Sequence similarities

Belongs to the WFIKKN family.

Contains 2 BPTI/Kunitz inhibitor domains.

Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Contains 1 Kazal-like domain.

Contains 1 NTR domain.

Contains 1 WAP domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3434 By similarity
Chain35 – 576542WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2
PRO_0000307820

Regions

Domain39 – 9254WAP
Domain126 – 17752Kazal-like
Domain210 – 30394Ig-like C2-type
Domain328 – 37851BPTI/Kunitz inhibitor 1
Domain386 – 43651BPTI/Kunitz inhibitor 2
Domain445 – 566122NTR

Sites

Site140 – 1412Reactive bond By similarity

Amino acid modifications

Glycosylation3191N-linked (GlcNAc...) Potential
Glycosylation5191N-linked (GlcNAc...) Potential
Disulfide bond46 ↔ 79 By similarity
Disulfide bond62 ↔ 83 By similarity
Disulfide bond66 ↔ 78 By similarity
Disulfide bond72 ↔ 88 By similarity
Disulfide bond134 ↔ 164 By similarity
Disulfide bond138 ↔ 157 By similarity
Disulfide bond146 ↔ 175 By similarity
Disulfide bond231 ↔ 287 By similarity
Disulfide bond328 ↔ 378 By similarity
Disulfide bond337 ↔ 361 By similarity
Disulfide bond353 ↔ 374 By similarity
Disulfide bond386 ↔ 436 By similarity
Disulfide bond395 ↔ 419 By similarity
Disulfide bond411 ↔ 432 By similarity
Disulfide bond445 ↔ 515 By similarity
Disulfide bond448 ↔ 517 By similarity
Disulfide bond459 ↔ 566 By similarity

Natural variations

Natural variant311R → Q. Ref.2
Corresponds to variant rs55700534 [ dbSNP | Ensembl ].
VAR_061984
Natural variant961V → M.
Corresponds to variant rs35300894 [ dbSNP | Ensembl ].
VAR_036692

Sequences

Sequence LengthMass (Da)Tools
Q8TEU8 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 08B4F2EDBE121F81

FASTA57663,941
        10         20         30         40         50         60 
MWAPRCRRFW SRWEQVAALL LLLLLLGVPP RSLALPPIRY SHAGICPNDM NPNLWVDAQS 

        70         80         90        100        110        120 
TCRRECETDQ ECETYEKCCP NVCGTKSCVA ARYMDVKGKK GPVGMPKEAT CDHFMCLQQG 

       130        140        150        160        170        180 
SECDIWDGQP VCKCKDRCEK EPSFTCASDG LTYYNRCYMD AEACSKGITL AVVTCRYHFT 

       190        200        210        220        230        240 
WPNTSPPPPE TTMHPTTASP ETPELDMAAP ALLNNPVHQS VTMGETVSFL CDVVGRPRPE 

       250        260        270        280        290        300 
ITWEKQLEDR ENVVMRPNHV RGNVVVTNIA QLVIYNAQLQ DAGIYTCTAR NVAGVLRADF 

       310        320        330        340        350        360 
PLSVVRGHQA AATSESSPNG TAFPAAECLK PPDSEDCGEE QTRWHFDAQA NNCLTFTFGH 

       370        380        390        400        410        420 
CHRNLNHFET YEACMLACMS GPLAACSLPA LQGPCKAYAP RWAYNSQTGQ CQSFVYGGCE 

       430        440        450        460        470        480 
GNGNNFESRE ACEESCPFPR GNQRCRACKP RQKLVTSFCR SDFVILGRVS ELTEEPDSGR 

       490        500        510        520        530        540 
ALVTVDEVLK DEKMGLKFLG QEPLEVTLLH VDWACPCPNV TVSEMPLIIM GEVDGGMAML 

       550        560        570 
RPDSFVGASS ARRVRKLREV MHKKTCDVLK EFLGLH 

« Hide

References

« Hide 'large scale' references
[1]"Distinct expression pattern of two related human proteins containing multiple types of protease-inhibitory modules."
Trexler M., Banyai L., Patthy L.
Biol. Chem. 383:223-228(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLN-31.
[3]"Regulation of myostatin in vivo by growth and differentiation factor-associated serum protein-1: a novel protein with protease inhibitor and follistatin domains."
Hill J.J., Qiu Y., Hewick R.M., Wolfman N.M.
Mol. Endocrinol. 17:1144-1154(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH MSTN.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF468657 mRNA. Translation: AAL77058.1.
AY358142 mRNA. Translation: AAQ88509.1.
CCDSCCDS11575.1.
RefSeqNP_783165.1. NM_175575.5.
UniGeneHs.211475.

3D structure databases

ProteinModelPortalQ8TEU8.
SMRQ8TEU8. Positions 129-173, 187-294, 337-436.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ8TEU8. 7 interactions.
MINTMINT-8044275.
STRING9606.ENSP00000311184.

Protein family/group databases

MEROPSI02.954.

PTM databases

PhosphoSiteQ8TEU8.

Polymorphism databases

DMDM74716081.

Proteomic databases

PaxDbQ8TEU8.
PRIDEQ8TEU8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000311378; ENSP00000311184; ENSG00000173714.
GeneID124857.
KEGGhsa:124857.
UCSCuc002isv.4. human.

Organism-specific databases

CTD124857.
GeneCardsGC17P048912.
HGNCHGNC:30916. WFIKKN2.
HPACAB025621.
HPA010953.
HPA011561.
MIM610895. gene.
neXtProtNX_Q8TEU8.
PharmGKBPA134864812.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG261628.
HOGENOMHOG000154855.
HOVERGENHBG108676.
InParanoidQ8TEU8.
OMACPNVCGT.
OrthoDBEOG754HSR.
PhylomeDBQ8TEU8.
TreeFamTF315349.

Gene expression databases

ArrayExpressQ8TEU8.
BgeeQ8TEU8.
CleanExHS_WFIKKN2.
GenevestigatorQ8TEU8.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
4.10.410.10. 2 hits.
4.10.75.10. 1 hit.
InterProIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003598. Ig_sub2.
IPR002350. Kazal_dom.
IPR001134. Netrin_domain.
IPR018933. Netrin_module_non-TIMP.
IPR002223. Prot_inh_Kunz-m.
IPR020901. Prtase_inh_Kunz-CS.
IPR008993. TIMP-like_OB-fold.
IPR008197. WAP-type_4-diS_core.
[Graphical view]
PfamPF07679. I-set. 1 hit.
PF07648. Kazal_2. 1 hit.
PF00014. Kunitz_BPTI. 2 hits.
PF01759. NTR. 1 hit.
PF00095. WAP. 1 hit.
[Graphical view]
PRINTSPR00759. BASICPTASE.
SMARTSM00408. IGc2. 1 hit.
SM00280. KAZAL. 1 hit.
SM00131. KU. 2 hits.
SM00217. WAP. 1 hit.
[Graphical view]
SUPFAMSSF50242. SSF50242. 1 hit.
SSF57256. SSF57256. 1 hit.
SSF57362. SSF57362. 2 hits.
PROSITEPS00280. BPTI_KUNITZ_1. 1 hit.
PS50279. BPTI_KUNITZ_2. 2 hits.
PS50835. IG_LIKE. 1 hit.
PS51465. KAZAL_2. 1 hit.
PS50189. NTR. 1 hit.
PS51390. WAP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi124857.
NextBio81402.
PROQ8TEU8.
SOURCESearch...

Entry information

Entry nameWFKN2_HUMAN
AccessionPrimary (citable) accession number: Q8TEU8
Secondary accession number(s): Q6UXZ9
Entry history
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: June 1, 2002
Last modified: July 9, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM