Q8TEL6 (TP4AP_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Short transient receptor potential channel 4-associated protein
Short name=Trp4-associated protein
Short name=Trpc4-associated protein

Alternative name(s):
Protein TAP1
TNF-receptor ubiquitous scaffolding/signaling protein
Short name=Protein TRUSS

Gene names

Name:	TRPC4AP
Synonyms:	C20orf188, TRRP4AP

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	797 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control. The DCX(TRUSS) complex specifically mediates the polyubiquitination and subsequent degradation of MYC. Also participates in the activation of NFKB1 in response to ligation of TNFRSF1A, possibly by linking TNFRSF1A to the IKK signalosome. Involved in JNK activation via its interaction with TRAF2. Also involved in elevation of endoplasmic reticulum Ca2+ storage reduction in response to CHRM1. Ref.6
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Constitutively associated with TNFRSF1A. Directly interacts with TRADD, TRAF2, CHUK, IKBKB and IKBKG. Interacts with TRPC1, TRPC4 and TRPC5 By similarity. Component of the DCX(TRUSS) E3 ubiquitin ligase complex, at least composed of CUL4A, DDB1, TRPC4AP/TRUSS and RBX1. Interacts with MYC. Ref.6
Sequence caution	The sequence BAB84932.1 differs from that shown. Reason: Frameshift at position 43.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Coding sequence diversity	Alternative splicing
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein ubiquitination Inferred from direct assay Ref.6. Source: UniProtKB ubiquitin-dependent protein catabolic process Inferred from direct assay Ref.6. Source: UniProtKB
Cellular_component	Cul4A-RING ubiquitin ligase complex Inferred from direct assay Ref.6. Source: UniProtKB
Molecular_function	phosphatase binding Inferred from direct assay PubMed 19389623. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PPP1CA	P62136	2	EBI-2559060,EBI-357253

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8TEL6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8TEL6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     533-797: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 797	797	Short transient receptor potential channel 4-associated protein		PRO_0000072641
Regions
	Region	1 – 400	400	Interaction with TNFRSF1A By similarity
Natural variations
	Alternative sequence	533 – 797	265	Missing in isoform 2.		VSP_003982

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 15, 2002. Version 2. Checksum: 999A8CA3B0D2CEEE	FASTA	797	90,852
	10 20 30 40 50 60 MAAAPVAAGS GAGRGRRSAA TVAAWGGWGG RPRPGNILLQ LRQGQLTGRG LVRAVQFTET 70 80 90 100 110 120 FLTERDKQSK WSGIPQLLLK LHTTSHLHSD FVECQNILKE ISPLLSMEAM AFVTEERKLT 130 140 150 160 170 180 QETTYPNTYI FDLFGGVDLL VEILMRPTIS IRGQKLKISD EMSKDCLSIL YNTCVCTEGV 190 200 210 220 230 240 TKRLAEKNDF VIFLFTLMTS KKTFLQTATL IEDILGVKKE MIRLDEVPNL SSLVSNFDQQ 250 260 270 280 290 300 QLANFCRILA VTISEMDTGN DDKHTLLAKN AQQKKSLSLG PSAAEINQAA LLSIPGFVER 310 320 330 340 350 360 LCKLATRKVS ESTGTASFLQ ELEEWYTWLD NALVLDALMR VANEESEHNQ ASIVFPPPGA 370 380 390 400 410 420 SEENGLPHTS ARTQLPQSMK IMHEIMYKLE VLYVLCVLLM GRQRNQVHRM IAEFKLIPGL 430 440 450 460 470 480 NNLFDKLIWR KHSASALVLH GHNQNCDCSP DITLKIQFLR LLQSFSDHHE NKYLLLNNQE 490 500 510 520 530 540 LNELSAISLK ANIPEVEAVL NTDRSLVCDG KRGLLTRLLQ VMKKEPAESS FRFWQARAVE 550 560 570 580 590 600 SFLRGTTSYA DQMFLLKRGL LEHILYCIVD SECKSRDVLQ SYFDLLGELM KFNVDAFKRF 610 620 630 640 650 660 NKYINTDAKF QVFLKQINSS LVDSNMLVRC VTLSLDRFEN QVDMKVAEVL SECRLLAYIS 670 680 690 700 710 720 QVPTQMSFLF RLINIIHVQT LTQENVSCLN TSLVILMLAR RKERLPLYLR LLQRMEHSKK 730 740 750 760 770 780 YPGFLLNNFH NLLRFWQQHY LHKDKDSTCL ENSSCISFSY WKETVSILLN PDRQSPSALV 790 SYIEEPYMDI DRDFTEE
	Isoform 2 [UniParc]. Checksum: D9E123C910E93950	FASTA	532	59,547
	10 20 30 40 50 60 MAAAPVAAGS GAGRGRRSAA TVAAWGGWGG RPRPGNILLQ LRQGQLTGRG LVRAVQFTET 70 80 90 100 110 120 FLTERDKQSK WSGIPQLLLK LHTTSHLHSD FVECQNILKE ISPLLSMEAM AFVTEERKLT 130 140 150 160 170 180 QETTYPNTYI FDLFGGVDLL VEILMRPTIS IRGQKLKISD EMSKDCLSIL YNTCVCTEGV 190 200 210 220 230 240 TKRLAEKNDF VIFLFTLMTS KKTFLQTATL IEDILGVKKE MIRLDEVPNL SSLVSNFDQQ 250 260 270 280 290 300 QLANFCRILA VTISEMDTGN DDKHTLLAKN AQQKKSLSLG PSAAEINQAA LLSIPGFVER 310 320 330 340 350 360 LCKLATRKVS ESTGTASFLQ ELEEWYTWLD NALVLDALMR VANEESEHNQ ASIVFPPPGA 370 380 390 400 410 420 SEENGLPHTS ARTQLPQSMK IMHEIMYKLE VLYVLCVLLM GRQRNQVHRM IAEFKLIPGL 430 440 450 460 470 480 NNLFDKLIWR KHSASALVLH GHNQNCDCSP DITLKIQFLR LLQSFSDHHE NKYLLLNNQE 490 500 510 520 530 LNELSAISLK ANIPEVEAVL NTDRSLVCDG KRGLLTRLLQ VMKKEPAESS FR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of long cDNA clones from human adult spleen. II. The complete sequences of 81 cDNA clones." Jikuya H., Takano J., Kikuno R., Hirosawa M., Nagase T., Nomura N., Ohara O. DNA Res. 10:49-57(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Spleen.
[2]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Cervix, Muscle and Prostate.
[5]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 238-797 (ISOFORM 1). Tissue: Mammary cancer.
[6]	"Myc protein is stabilized by suppression of a novel E3 ligase complex in cancer cells." Choi S.H., Wright J.B., Gerber S.A., Cole M.D. Genes Dev. 24:1236-1241(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN A DCX (DDB1-CUL4-X-BOX) E3 UBIQUITIN-PROTEIN LIGASE COMPLEX, INTERACTION WITH MYC.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK074106 mRNA. Translation: BAB84932.1. Frameshift. AL132825 Genomic DNA. Translation: CAC14946.1. CH471077 Genomic DNA. Translation: EAW76229.1. CH471077 Genomic DNA. Translation: EAW76234.1. BC001323 mRNA. Translation: AAH01323.1. BC008836 mRNA. Translation: AAH08836.2. BC013144 mRNA. Translation: AAH13144.1. AL117480 mRNA. Translation: CAB55953.1.
IPI	IPI00152769. IPI00219534.
PIR	T17263.
RefSeq	NP_056453.1. NM_015638.2. NP_955400.1. NM_199368.1.
UniGene	Hs.168073.
3D structure databases
ProteinModelPortal	Q8TEL6.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-42719N.
IntAct	Q8TEL6. 3 interactions.
MINT	MINT-2844857.
STRING	9606.ENSP00000252015.
PTM databases
PhosphoSite	Q8TEL6.
Polymorphism databases
DMDM	25091357.
Proteomic databases
PaxDb	Q8TEL6.
PRIDE	Q8TEL6.
Protocols and materials databases
DNASU	26133.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000252015; ENSP00000252015; ENSG00000100991.
GeneID	26133.
KEGG	hsa:26133.
UCSC	uc002xbk.3. human.
Organism-specific databases
CTD	26133.
GeneCards	GC20M033590.
HGNC	HGNC:16181. TRPC4AP.
HPA	HPA051197.
MIM	608430. gene.
neXtProt	NX_Q8TEL6.
PharmGKB	PA25730.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG42180.
HOGENOM	HOG000231794.
HOVERGEN	HBG059886.
InParanoid	Q8TEL6.
KO	K11796.
OMA	QESTFPN.
OrthoDB	EOG49KFQ0.
PhylomeDB	Q8TEL6.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
ArrayExpress	Q8TEL6.
Bgee	Q8TEL6.
CleanEx	HS_TRPC4AP.
Genevestigator	Q8TEL6.
GermOnline	ENSG00000100991. Homo sapiens.
Family and domain databases
InterPro	IPR022162. DUF3689. [Graphical view]
Pfam	PF12463. DUF3689. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	TRPC4AP. human.
GenomeRNAi	26133.
NextBio	48157.
SOURCE	Search...

Entry information

Entry name

TP4AP_HUMAN

Accession

Primary (citable) accession number: Q8TEL6
Secondary accession number(s): E1P5Q1 , Q96H82, Q9BVB8, Q9H429, Q9UFS6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	November 15, 2002
Last modified:	May 1, 2013
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.