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Protein

Short transient receptor potential channel 4-associated protein

Gene

TRPC4AP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control. The DCX(TRUSS) complex specifically mediates the polyubiquitination and subsequent degradation of MYC. Also participates in the activation of NFKB1 in response to ligation of TNFRSF1A, possibly by linking TNFRSF1A to the IKK signalosome. Involved in JNK activation via its interaction with TRAF2. Also involved in elevation of endoplasmic reticulum Ca2+ storage reduction in response to CHRM1.1 Publication

Pathwayi

GO - Molecular functioni

  1. phosphatase binding Source: UniProtKB

GO - Biological processi

  1. calcium ion transmembrane transport Source: Reactome
  2. ion transmembrane transport Source: Reactome
  3. protein ubiquitination Source: UniProtKB
  4. transmembrane transport Source: Reactome
  5. ubiquitin-dependent protein catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiREACT_169333. TRP channels.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Short transient receptor potential channel 4-associated protein
Short name:
Trp4-associated protein
Short name:
Trpc4-associated protein
Alternative name(s):
Protein TAP1
TNF-receptor ubiquitous scaffolding/signaling protein
Short name:
Protein TRUSS
Gene namesi
Name:TRPC4AP
Synonyms:C20orf188, TRRP4AP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 20

Organism-specific databases

HGNCiHGNC:16181. TRPC4AP.

Subcellular locationi

GO - Cellular componenti

  1. Cul4A-RING E3 ubiquitin ligase complex Source: UniProtKB
  2. plasma membrane Source: Reactome
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA25730.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 797796Short transient receptor potential channel 4-associated proteinPRO_0000072641Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ8TEL6.
PaxDbiQ8TEL6.
PRIDEiQ8TEL6.

PTM databases

PhosphoSiteiQ8TEL6.

Expressioni

Gene expression databases

BgeeiQ8TEL6.
CleanExiHS_TRPC4AP.
GenevestigatoriQ8TEL6.

Organism-specific databases

HPAiHPA051197.

Interactioni

Subunit structurei

Constitutively associated with TNFRSF1A. Directly interacts with TRADD, TRAF2, CHUK, IKBKB and IKBKG. Interacts with TRPC1, TRPC4 and TRPC5 (By similarity). Component of the DCX(TRUSS) E3 ubiquitin ligase complex, at least composed of CUL4A, DDB1, TRPC4AP/TRUSS and RBX1. Interacts directly with DDB1. Interacts with MYC.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PPP1CAP621362EBI-2559060,EBI-357253

Protein-protein interaction databases

BioGridi117569. 22 interactions.
DIPiDIP-42719N.
IntActiQ8TEL6. 5 interactions.
MINTiMINT-2844857.
STRINGi9606.ENSP00000252015.

Structurei

3D structure databases

ProteinModelPortaliQ8TEL6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 400399Interaction with TNFRSF1ABy similarityAdd
BLAST

Phylogenomic databases

eggNOGiNOG42180.
GeneTreeiENSGT00390000018330.
HOGENOMiHOG000231794.
HOVERGENiHBG059886.
InParanoidiQ8TEL6.
KOiK11796.
OMAiHYLNKDK.
OrthoDBiEOG7P02H9.
PhylomeDBiQ8TEL6.
TreeFamiTF329145.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR022162. DUF3689.
[Graphical view]
PfamiPF12463. DUF3689. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 4 hits.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8TEL6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAPVAAGS GAGRGRRSAA TVAAWGGWGG RPRPGNILLQ LRQGQLTGRG
60 70 80 90 100
LVRAVQFTET FLTERDKQSK WSGIPQLLLK LHTTSHLHSD FVECQNILKE
110 120 130 140 150
ISPLLSMEAM AFVTEERKLT QETTYPNTYI FDLFGGVDLL VEILMRPTIS
160 170 180 190 200
IRGQKLKISD EMSKDCLSIL YNTCVCTEGV TKRLAEKNDF VIFLFTLMTS
210 220 230 240 250
KKTFLQTATL IEDILGVKKE MIRLDEVPNL SSLVSNFDQQ QLANFCRILA
260 270 280 290 300
VTISEMDTGN DDKHTLLAKN AQQKKSLSLG PSAAEINQAA LLSIPGFVER
310 320 330 340 350
LCKLATRKVS ESTGTASFLQ ELEEWYTWLD NALVLDALMR VANEESEHNQ
360 370 380 390 400
ASIVFPPPGA SEENGLPHTS ARTQLPQSMK IMHEIMYKLE VLYVLCVLLM
410 420 430 440 450
GRQRNQVHRM IAEFKLIPGL NNLFDKLIWR KHSASALVLH GHNQNCDCSP
460 470 480 490 500
DITLKIQFLR LLQSFSDHHE NKYLLLNNQE LNELSAISLK ANIPEVEAVL
510 520 530 540 550
NTDRSLVCDG KRGLLTRLLQ VMKKEPAESS FRFWQARAVE SFLRGTTSYA
560 570 580 590 600
DQMFLLKRGL LEHILYCIVD SECKSRDVLQ SYFDLLGELM KFNVDAFKRF
610 620 630 640 650
NKYINTDAKF QVFLKQINSS LVDSNMLVRC VTLSLDRFEN QVDMKVAEVL
660 670 680 690 700
SECRLLAYIS QVPTQMSFLF RLINIIHVQT LTQENVSCLN TSLVILMLAR
710 720 730 740 750
RKERLPLYLR LLQRMEHSKK YPGFLLNNFH NLLRFWQQHY LHKDKDSTCL
760 770 780 790
ENSSCISFSY WKETVSILLN PDRQSPSALV SYIEEPYMDI DRDFTEE
Length:797
Mass (Da):90,852
Last modified:November 15, 2002 - v2
Checksum:i999A8CA3B0D2CEEE
GO
Isoform 2 (identifier: Q8TEL6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     533-797: Missing.

Note: No experimental confirmation available.

Show »
Length:532
Mass (Da):59,547
Checksum:iD9E123C910E93950
GO
Isoform 3 (identifier: Q8TEL6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     351-358: Missing.

Show »
Length:789
Mass (Da):90,043
Checksum:i8FF952722B28B257
GO

Sequence cautioni

The sequence BAB84932.1 differs from that shown. Reason: Frameshift at position 43. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei351 – 3588Missing in isoform 3. 1 PublicationVSP_054231
Alternative sequencei533 – 797265Missing in isoform 2. 1 PublicationVSP_003982Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK074106 mRNA. Translation: BAB84932.1. Frameshift.
AB590540 mRNA. Translation: BAJ20695.1.
AL132825 Genomic DNA. Translation: CAC14946.1.
CH471077 Genomic DNA. Translation: EAW76233.1.
CH471077 Genomic DNA. Translation: EAW76235.1.
CH471077 Genomic DNA. Translation: EAW76229.1.
CH471077 Genomic DNA. Translation: EAW76234.1.
BC001323 mRNA. Translation: AAH01323.1.
BC008836 mRNA. Translation: AAH08836.2.
BC013144 mRNA. Translation: AAH13144.1.
AL117480 mRNA. Translation: CAB55953.1.
CCDSiCCDS13246.1. [Q8TEL6-1]
CCDS46591.1. [Q8TEL6-3]
PIRiT17263.
RefSeqiNP_056453.1. NM_015638.2. [Q8TEL6-1]
NP_955400.1. NM_199368.1. [Q8TEL6-3]
UniGeneiHs.168073.

Genome annotation databases

EnsembliENST00000252015; ENSP00000252015; ENSG00000100991. [Q8TEL6-1]
ENST00000451813; ENSP00000400614; ENSG00000100991. [Q8TEL6-3]
GeneIDi26133.
KEGGihsa:26133.
UCSCiuc002xbk.3. human. [Q8TEL6-1]
uc002xbl.3. human.

Polymorphism databases

DMDMi25091357.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK074106 mRNA. Translation: BAB84932.1. Frameshift.
AB590540 mRNA. Translation: BAJ20695.1.
AL132825 Genomic DNA. Translation: CAC14946.1.
CH471077 Genomic DNA. Translation: EAW76233.1.
CH471077 Genomic DNA. Translation: EAW76235.1.
CH471077 Genomic DNA. Translation: EAW76229.1.
CH471077 Genomic DNA. Translation: EAW76234.1.
BC001323 mRNA. Translation: AAH01323.1.
BC008836 mRNA. Translation: AAH08836.2.
BC013144 mRNA. Translation: AAH13144.1.
AL117480 mRNA. Translation: CAB55953.1.
CCDSiCCDS13246.1. [Q8TEL6-1]
CCDS46591.1. [Q8TEL6-3]
PIRiT17263.
RefSeqiNP_056453.1. NM_015638.2. [Q8TEL6-1]
NP_955400.1. NM_199368.1. [Q8TEL6-3]
UniGeneiHs.168073.

3D structure databases

ProteinModelPortaliQ8TEL6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117569. 22 interactions.
DIPiDIP-42719N.
IntActiQ8TEL6. 5 interactions.
MINTiMINT-2844857.
STRINGi9606.ENSP00000252015.

PTM databases

PhosphoSiteiQ8TEL6.

Polymorphism databases

DMDMi25091357.

Proteomic databases

MaxQBiQ8TEL6.
PaxDbiQ8TEL6.
PRIDEiQ8TEL6.

Protocols and materials databases

DNASUi26133.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000252015; ENSP00000252015; ENSG00000100991. [Q8TEL6-1]
ENST00000451813; ENSP00000400614; ENSG00000100991. [Q8TEL6-3]
GeneIDi26133.
KEGGihsa:26133.
UCSCiuc002xbk.3. human. [Q8TEL6-1]
uc002xbl.3. human.

Organism-specific databases

CTDi26133.
GeneCardsiGC20M033590.
HGNCiHGNC:16181. TRPC4AP.
HPAiHPA051197.
MIMi608430. gene.
neXtProtiNX_Q8TEL6.
PharmGKBiPA25730.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG42180.
GeneTreeiENSGT00390000018330.
HOGENOMiHOG000231794.
HOVERGENiHBG059886.
InParanoidiQ8TEL6.
KOiK11796.
OMAiHYLNKDK.
OrthoDBiEOG7P02H9.
PhylomeDBiQ8TEL6.
TreeFamiTF329145.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiREACT_169333. TRP channels.

Miscellaneous databases

ChiTaRSiTRPC4AP. human.
GeneWikiiTRPC4AP.
GenomeRNAii26133.
NextBioi48157.
PROiQ8TEL6.
SOURCEiSearch...

Gene expression databases

BgeeiQ8TEL6.
CleanExiHS_TRPC4AP.
GenevestigatoriQ8TEL6.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR022162. DUF3689.
[Graphical view]
PfamiPF12463. DUF3689. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 4 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
  2. "The DNA sequence and comparative analysis of human chromosome 20."
    Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E.
    , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
    Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Cervix, Muscle and Prostate.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 238-797 (ISOFORM 1).
    Tissue: Mammary cancer.
  6. "Myc protein is stabilized by suppression of a novel E3 ligase complex in cancer cells."
    Choi S.H., Wright J.B., Gerber S.A., Cole M.D.
    Genes Dev. 24:1236-1241(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN A DCX (DDB1-CUL4-X-BOX) E3 UBIQUITIN-PROTEIN LIGASE COMPLEX, INTERACTION WITH MYC.
  7. "A promiscuous alpha-helical motif anchors viral hijackers and substrate receptors to the CUL4-DDB1 ubiquitin ligase machinery."
    Li T., Robert E.I., van Breugel P.C., Strubin M., Zheng N.
    Nat. Struct. Mol. Biol. 17:105-111(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DDB1.
  8. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTP4AP_HUMAN
AccessioniPrimary (citable) accession number: Q8TEL6
Secondary accession number(s): E1P5Q0
, E1P5Q1, Q96H82, Q9BVB8, Q9H429, Q9UFS6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: November 15, 2002
Last modified: January 7, 2015
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.