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Protein

Putative E3 ubiquitin-protein ligase SH3RF2

Gene

SH3RF2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits PPP1CA phosphatase activity. May be a E3 ubiquitin-protein ligase (Potential). May play a role in cardiac function.Curated2 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri12 – 53RING-typePROSITE-ProRule annotationAdd BLAST42

GO - Molecular functioni

  • ligase activity Source: UniProtKB-KW
  • phosphatase binding Source: UniProtKB
  • protein phosphatase 1 binding Source: UniProtKB
  • protein phosphatase inhibitor activity Source: UniProtKB-KW
  • zinc ion binding Source: InterPro

GO - Biological processi

  • negative regulation of phosphatase activity Source: UniProtKB
  • protein ubiquitination Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase, Protein phosphatase inhibitor

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative E3 ubiquitin-protein ligase SH3RF2 (EC:6.3.2.-)
Alternative name(s):
Heart protein phosphatase 1-binding protein
Short name:
HEPP1
Protein phosphatase 1 regulatory subunit 39
RING finger protein 158
SH3 domain-containing RING finger protein 2
Gene namesi
Name:SH3RF2
Synonyms:PPP1R39, RNF158
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:26299. SH3RF2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi643V → G: Significant loss of interaction with PPP1CA. Significant loss of interaction with PPP1CA; when associated with G-645. 1 Publication1
Mutagenesisi645F → G: Significant loss of interaction with PPP1CA. Significant loss of interaction with PPP1CA; when associated with G-643. 1 Publication1

Organism-specific databases

DisGeNETi153769.
OpenTargetsiENSG00000156463.
PharmGKBiPA134944983.

Polymorphism and mutation databases

BioMutaiSH3RF2.
DMDMi296452957.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002695121 – 729Putative E3 ubiquitin-protein ligase SH3RF2Add BLAST729

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei649PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8TEC5.
PaxDbiQ8TEC5.
PeptideAtlasiQ8TEC5.
PRIDEiQ8TEC5.
TopDownProteomicsiQ8TEC5-1. [Q8TEC5-1]

PTM databases

iPTMnetiQ8TEC5.
PhosphoSitePlusiQ8TEC5.

Expressioni

Tissue specificityi

Heart (at protein level). Heart and testis. In the heart, present in the apex, left atrium, right atrium, left ventricle and right ventricle, but not in the aorta.1 Publication

Gene expression databases

BgeeiENSG00000156463.
CleanExiHS_SH3RF2.
GenevisibleiQ8TEC5. HS.

Organism-specific databases

HPAiHPA035903.
HPA058119.

Interactioni

Subunit structurei

Interacts with FASLG and PPP1CA.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BANPQ8N9N53EBI-2130111,EBI-744695
PPP1CAP621363EBI-2130111,EBI-357253

GO - Molecular functioni

  • phosphatase binding Source: UniProtKB
  • protein phosphatase 1 binding Source: UniProtKB

Protein-protein interaction databases

BioGridi127517. 33 interactors.
IntActiQ8TEC5. 9 interactors.
STRINGi9606.ENSP00000352028.

Structurei

3D structure databases

ProteinModelPortaliQ8TEC5.
SMRiQ8TEC5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini125 – 184SH3 1PROSITE-ProRule annotationAdd BLAST60
Domaini187 – 252SH3 2PROSITE-ProRule annotationAdd BLAST66
Domaini380 – 441SH3 3PROSITE-ProRule annotationAdd BLAST62

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni641 – 646Interaction with PPP1CA6

Domaini

The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.By similarity

Sequence similaritiesi

Belongs to the SH3RF family.Curated
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation
Contains 3 SH3 domains.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri12 – 53RING-typePROSITE-ProRule annotationAdd BLAST42

Keywords - Domaini

Repeat, SH3 domain, Zinc-finger

Phylogenomic databases

eggNOGiENOG410ITG6. Eukaryota.
ENOG410XS5R. LUCA.
GeneTreeiENSGT00760000119190.
HOGENOMiHOG000060125.
HOVERGENiHBG082418.
InParanoidiQ8TEC5.
KOiK12171.
OMAiNMFVALH.
OrthoDBiEOG091G09B6.
PhylomeDBiQ8TEC5.
TreeFamiTF105571.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR011511. SH3_2.
IPR001452. SH3_domain.
IPR027370. Znf-RING_LisH.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF00018. SH3_1. 1 hit.
PF07653. SH3_2. 1 hit.
PF14604. SH3_9. 1 hit.
PF13445. zf-RING_UBOX. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00184. RING. 1 hit.
SM00326. SH3. 3 hits.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 3 hits.
PROSITEiPS50002. SH3. 3 hits.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8TEC5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDDLTLLDLL ECPVCFEKLD VTAKVLPCQH TFCKPCLQRV FKAHKELRCP
60 70 80 90 100
ECRTPVFSNI EALPANLLLV RLLDGVRSGQ SSGRGGSFRR PGTMTLQDGR
110 120 130 140 150
KSRTNPRRLQ ASPFRLVPNV RIHMDGVPRA KALCNYRGQN PGDLRFNKGD
160 170 180 190 200
IILLRRQLDE NWYQGEINGI SGNFPASSVE VIKQLPQPPP LCRALYNFDL
210 220 230 240 250
RGKDKSENQD CLTFLKDDII TVISRVDENW AEGKLGDKVG IFPILFVEPN
260 270 280 290 300
LTARHLLEKN KGRQSSRTKN LSLVSSSSRG NTSTLRRGPG SRRKVPGQFS
310 320 330 340 350
ITTALNTLNR MVHSPSGRHM VEISTPVLIS SSNPSVITQP MEKADVPSSC
360 370 380 390 400
VGQVSTYHPA PVSPGHSTAV VSLPGSQQHL SANMFVALHS YSAHGPDELD
410 420 430 440 450
LQKGEGVRVL GKCQDGWLRG VSLVTGRVGI FPNNYVIPIF RKTSSFPDSR
460 470 480 490 500
SPGLYTTWTL STSSVSSQGS ISEGDPRQSR PFKSVFVPTA IVNPVRSTAG
510 520 530 540 550
PGTLGQGSLR KGRSSMRKNG SLQRPLQSGI PTLVVGSLRR SPTMVLRPQQ
560 570 580 590 600
FQFYQPQGIP SSPSAVVVEM GSKPALTGEP ALTCISRGSE AWIHSAASSL
610 620 630 640 650
IMEDKEIPIK SEPLPKPPAS APPSILVKPE NSRNGIEKQV KTVRFQNYSP
660 670 680 690 700
PPTKHYTSHP TSGKPEQPAT LKASQPEAAS LGPEMTVLFA HRSGCHSGQQ
710 720
TDLRRKSALG KATTLVSTAS GTQTVFPSK
Length:729
Mass (Da):79,320
Last modified:May 18, 2010 - v3
Checksum:iF85CCC0188DA8477
GO
Isoform 2 (identifier: Q8TEC5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-508: Missing.
     509-519: LRKGRSSMRKN → MRKSKWWQRD

Show »
Length:220
Mass (Da):23,799
Checksum:i299343322F39CAB2
GO
Isoform 3 (identifier: Q8TEC5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-543: Missing.

Show »
Length:186
Mass (Da):19,895
Checksum:i1FCF3EE1BF6C9316
GO

Sequence cautioni

The sequence AAH31650 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAB85025 differs from that shown. Reason: Erroneous termination at position 458. Translated as Trp.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti148K → R in BAB85025 (PubMed:14702039).Curated1
Sequence conflicti168N → S in BAB85025 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05211816F → S.Corresponds to variant rs34739859dbSNPEnsembl.1
Natural variantiVAR_052119174F → V.Corresponds to variant rs34942619dbSNPEnsembl.1
Natural variantiVAR_029788267R → C.2 PublicationsCorresponds to variant rs758037dbSNPEnsembl.1
Natural variantiVAR_052120477R → Q.Corresponds to variant rs35165046dbSNPEnsembl.1
Natural variantiVAR_029789592W → R.2 PublicationsCorresponds to variant rs2962525dbSNPEnsembl.1
Natural variantiVAR_029790687V → I.1 PublicationCorresponds to variant rs11435dbSNPEnsembl.1
Natural variantiVAR_029791710G → A.2 PublicationsCorresponds to variant rs1056149dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0406621 – 543Missing in isoform 3. 1 PublicationAdd BLAST543
Alternative sequenceiVSP_0220581 – 508Missing in isoform 2. 2 PublicationsAdd BLAST508
Alternative sequenceiVSP_022059509 – 519LRKGRSSMRKN → MRKSKWWQRD in isoform 2. 2 PublicationsAdd BLAST11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK058046 mRNA. Translation: BAB71639.1.
AK074234 mRNA. Translation: BAB85025.1. Different termination.
AK292576 mRNA. Translation: BAF85265.1.
AC005216 Genomic DNA. No translation available.
AC005351 Genomic DNA. No translation available.
AC011359 Genomic DNA. No translation available.
AC091887 Genomic DNA. No translation available.
BC031650 mRNA. Translation: AAH31650.2. Different initiation.
BC073914 mRNA. Translation: AAH73914.1.
BC125106 mRNA. Translation: AAI25107.1.
CCDSiCCDS4280.1. [Q8TEC5-1]
RefSeqiNP_689763.3. NM_152550.3.
XP_006714820.1. XM_006714757.1. [Q8TEC5-1]
XP_011535871.1. XM_011537569.2. [Q8TEC5-1]
XP_016864625.1. XM_017009136.1. [Q8TEC5-1]
UniGeneiHs.443728.

Genome annotation databases

EnsembliENST00000359120; ENSP00000352028; ENSG00000156463. [Q8TEC5-1]
ENST00000511217; ENSP00000424497; ENSG00000156463. [Q8TEC5-1]
GeneIDi153769.
KEGGihsa:153769.
UCSCiuc003lnt.4. human. [Q8TEC5-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK058046 mRNA. Translation: BAB71639.1.
AK074234 mRNA. Translation: BAB85025.1. Different termination.
AK292576 mRNA. Translation: BAF85265.1.
AC005216 Genomic DNA. No translation available.
AC005351 Genomic DNA. No translation available.
AC011359 Genomic DNA. No translation available.
AC091887 Genomic DNA. No translation available.
BC031650 mRNA. Translation: AAH31650.2. Different initiation.
BC073914 mRNA. Translation: AAH73914.1.
BC125106 mRNA. Translation: AAI25107.1.
CCDSiCCDS4280.1. [Q8TEC5-1]
RefSeqiNP_689763.3. NM_152550.3.
XP_006714820.1. XM_006714757.1. [Q8TEC5-1]
XP_011535871.1. XM_011537569.2. [Q8TEC5-1]
XP_016864625.1. XM_017009136.1. [Q8TEC5-1]
UniGeneiHs.443728.

3D structure databases

ProteinModelPortaliQ8TEC5.
SMRiQ8TEC5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi127517. 33 interactors.
IntActiQ8TEC5. 9 interactors.
STRINGi9606.ENSP00000352028.

PTM databases

iPTMnetiQ8TEC5.
PhosphoSitePlusiQ8TEC5.

Polymorphism and mutation databases

BioMutaiSH3RF2.
DMDMi296452957.

Proteomic databases

MaxQBiQ8TEC5.
PaxDbiQ8TEC5.
PeptideAtlasiQ8TEC5.
PRIDEiQ8TEC5.
TopDownProteomicsiQ8TEC5-1. [Q8TEC5-1]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000359120; ENSP00000352028; ENSG00000156463. [Q8TEC5-1]
ENST00000511217; ENSP00000424497; ENSG00000156463. [Q8TEC5-1]
GeneIDi153769.
KEGGihsa:153769.
UCSCiuc003lnt.4. human. [Q8TEC5-1]

Organism-specific databases

CTDi153769.
DisGeNETi153769.
GeneCardsiSH3RF2.
HGNCiHGNC:26299. SH3RF2.
HPAiHPA035903.
HPA058119.
MIMi613377. gene.
neXtProtiNX_Q8TEC5.
OpenTargetsiENSG00000156463.
PharmGKBiPA134944983.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410ITG6. Eukaryota.
ENOG410XS5R. LUCA.
GeneTreeiENSGT00760000119190.
HOGENOMiHOG000060125.
HOVERGENiHBG082418.
InParanoidiQ8TEC5.
KOiK12171.
OMAiNMFVALH.
OrthoDBiEOG091G09B6.
PhylomeDBiQ8TEC5.
TreeFamiTF105571.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

ChiTaRSiSH3RF2. human.
GenomeRNAii153769.
PROiQ8TEC5.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000156463.
CleanExiHS_SH3RF2.
GenevisibleiQ8TEC5. HS.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR011511. SH3_2.
IPR001452. SH3_domain.
IPR027370. Znf-RING_LisH.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF00018. SH3_1. 1 hit.
PF07653. SH3_2. 1 hit.
PF14604. SH3_9. 1 hit.
PF13445. zf-RING_UBOX. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00184. RING. 1 hit.
SM00326. SH3. 3 hits.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 3 hits.
PROSITEiPS50002. SH3. 3 hits.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSH3R2_HUMAN
AccessioniPrimary (citable) accession number: Q8TEC5
Secondary accession number(s): A8K961
, Q08AM9, Q6GMR9, Q8N5S8, Q96LP8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: May 18, 2010
Last modified: November 30, 2016
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.