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Protein

D-aminoacyl-tRNA deacylase 1

Gene

DTD1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Possible ATPase (PubMed:15653697) involved in DNA replication, may facilitate loading of CDC45 onto pre-replication complexes (PubMed:20065034).2 Publications
An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Acts via tRNA-based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality.By similarity

Catalytic activityi

Glycyl-tRNA(Ala) + H2O = glycine + tRNA(Ala).By similarity
A D-aminoacyl-tRNA + H2O = a D-amino acid + tRNA.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi4Magnesium; via carbonyl oxygen1 Publication1
Metal bindingi6Magnesium1 Publication1
Metal bindingi28Magnesium; via carbonyl oxygen1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Hydrolase, RNA-binding, tRNA-binding
Biological processDNA replication
LigandMetal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
D-aminoacyl-tRNA deacylase 1By similarity (EC:3.1.1.96By similarity)
Short name:
DTD
Alternative name(s):
DNA-unwinding element-binding protein B1 Publication
Short name:
DUE-B1 Publication
Gly-tRNA(Ala) deacylaseBy similarity (EC:3.1.1.-By similarity)
Histidyl-tRNA synthase-related
Gene namesi
Name:DTD1
Synonyms:C20orf88, DUEB, HARS2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

EuPathDBiHostDB:ENSG00000125821.11.
HGNCiHGNC:16219. DTD1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi92675.
OpenTargetsiENSG00000125821.
PharmGKBiPA162384107.

Polymorphism and mutation databases

BioMutaiDTD1.
DMDMi29427856.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001646261 – 209D-aminoacyl-tRNA deacylase 1Add BLAST209

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei197PhosphoserineCombined sources1
Modified residuei204PhosphoserineBy similarity1
Modified residuei205PhosphoserineCombined sources1

Post-translational modificationi

Preferentially phosphorylated in cells arrested early in S phase (PubMed:15653697). Phosphorylation in the C-terminus weakens the interaction with CDC45 (PubMed:20065034).2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8TEA8.
MaxQBiQ8TEA8.
PaxDbiQ8TEA8.
PeptideAtlasiQ8TEA8.
PRIDEiQ8TEA8.

PTM databases

iPTMnetiQ8TEA8.
PhosphoSitePlusiQ8TEA8.

Expressioni

Tissue specificityi

Expressed in many adult and fetal tissues. Highest levels in testis, ovary, spleen and in adult and fetal brain.1 Publication

Gene expression databases

BgeeiENSG00000125821.
CleanExiHS_DTD1.
HS_HARS2.
ExpressionAtlasiQ8TEA8. baseline and differential.
GenevisibleiQ8TEA8. HS.

Organism-specific databases

HPAiHPA040981.
HPA042653.

Interactioni

Subunit structurei

Homodimer (PubMed15653697). Interacts with CDC45 and TOPBP1 (PubMed:20065034).3 Publications

Protein-protein interaction databases

BioGridi124965. 15 interactors.
IntActiQ8TEA8. 1 interactor.
STRINGi9606.ENSP00000366672.

Structurei

Secondary structure

1209
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 15Combined sources14
Beta strandi18 – 32Combined sources15
Helixi39 – 51Combined sources13
Helixi67 – 70Combined sources4
Beta strandi73 – 78Combined sources6
Helixi80 – 82Combined sources3
Beta strandi87 – 90Combined sources4
Helixi99 – 116Combined sources18
Helixi119 – 121Combined sources3
Beta strandi122 – 124Combined sources3
Beta strandi131 – 146Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2OKVX-ray2.00A/B/C/D1-209[»]
ProteinModelPortaliQ8TEA8.
SMRiQ8TEA8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8TEA8.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi139 – 140Gly-cisPro motif, important for rejection of L-amino acidsBy similarity2

Domaini

A Gly-cisPro motif from one monomer fits into the active site of the other monomer to allow specific chiral rejection of L-amino acids.By similarity

Sequence similaritiesi

Belongs to the DTD family.Curated

Phylogenomic databases

eggNOGiKOG3323. Eukaryota.
COG1490. LUCA.
GeneTreeiENSGT00390000008298.
HOVERGENiHBG039436.
InParanoidiQ8TEA8.
KOiK07560.
OMAiELAQPFY.
PhylomeDBiQ8TEA8.
TreeFamiTF314886.

Family and domain databases

CDDicd00563. Dtyr_deacylase. 1 hit.
Gene3Di3.50.80.10. 2 hits.
HAMAPiMF_00518. Deacylase_Dtd. 1 hit.
InterProiView protein in InterPro
IPR003732. Daa-tRNA_deacyls_DTD.
IPR023509. DTD-like_dom.
PANTHERiPTHR10472. PTHR10472. 1 hit.
PfamiView protein in Pfam
PF02580. Tyr_Deacylase. 1 hit.
SUPFAMiSSF69500. SSF69500. 1 hit.
TIGRFAMsiTIGR00256. TIGR00256. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8TEA8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAVVQRVTR ASVTVGGEQI SAIGRGICVL LGISLEDTQK ELEHMVRKIL
60 70 80 90 100
NLRVFEDESG KHWSKSVMDK QYEILCVSQF TLQCVLKGNK PDFHLAMPTE
110 120 130 140 150
QAEGFYNSFL EQLRKTYRPE LIKDGKFGAY MQVHIQNDGP VTIELESPAP
160 170 180 190 200
GTATSDPKQL SKLEKQQQRK EKTRAKGPSE SSKERNTPRK EDRSASSGAE

GDVSSEREP
Length:209
Mass (Da):23,424
Last modified:March 28, 2003 - v2
Checksum:iF006ED14974ACC92
GO

Sequence cautioni

The sequence BAB85044 differs from that shown. Presence of Alu-repeat DNA.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti94H → N in AAH45167 (PubMed:15489334).Curated1
Sequence conflicti134H → R in AAL57046 (PubMed:12392168).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF332356 mRNA. Translation: AAL57046.1.
AK074304 mRNA. Translation: BAB85044.1. Sequence problems.
AK291440 mRNA. Translation: BAF84129.1.
AL121900, AL121780 Genomic DNA. Translation: CAH73147.1.
AL121780, AL121900 Genomic DNA. Translation: CAI15669.1.
CH471133 Genomic DNA. Translation: EAX10227.1.
BC000599 mRNA. No translation available.
CH471133 Genomic DNA. Translation: EAX10228.1.
BC045167 mRNA. Translation: AAH45167.1.
BC100923 mRNA. Translation: AAI00924.1.
BC100924 mRNA. Translation: AAI00925.1.
BC100925 mRNA. Translation: AAI00926.1.
CCDSiCCDS13138.1.
RefSeqiNP_001304972.1. NM_001318043.1.
NP_543010.3. NM_080820.5.
UniGeneiHs.659442.

Genome annotation databases

EnsembliENST00000377452; ENSP00000366672; ENSG00000125821.
GeneIDi92675.
KEGGihsa:92675.
UCSCiuc002wrf.5. human.

Similar proteinsi

Entry informationi

Entry nameiDTD1_HUMAN
AccessioniPrimary (citable) accession number: Q8TEA8
Secondary accession number(s): A8K5X5
, D3DW37, Q496D1, Q5W184, Q8WXU8, Q9BW67, Q9H464, Q9H474
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: March 28, 2003
Last modified: September 27, 2017
This is version 135 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families