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Q8TEA8

- DTD1_HUMAN

UniProt

Q8TEA8 - DTD1_HUMAN

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Protein

D-tyrosyl-tRNA(Tyr) deacylase 1

Gene

DTD1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

ATPase involved in DNA replication, may facilitate loading of CDC45 onto pre-replication complexes. May hydrolyze D-tyrosyl-tRNA(Tyr) into D-tyrosine and free tRNA(Tyr), a possible defense mechanism against a harmful effect of D-tyrosine.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi4 – 41Magnesium; via carbonyl oxygen
Metal bindingi6 – 61Magnesium
Metal bindingi28 – 281Magnesium; via carbonyl oxygen
Active sitei81 – 811NucleophileBy similarity

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. hydrolase activity, acting on ester bonds Source: InterPro
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. D-amino acid catabolic process Source: InterPro
  2. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
D-tyrosyl-tRNA(Tyr) deacylase 1 (EC:3.1.-.-)
Alternative name(s):
DNA-unwinding element-binding protein B
Short name:
DUE-B
Histidyl-tRNA synthase-related
Gene namesi
Name:DTD1
Synonyms:C20orf88, DUEB, HARS2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 20

Organism-specific databases

HGNCiHGNC:16219. DTD1.

Subcellular locationi

Nucleus 2 Publications. Cytoplasm Curated
Note: Associated with chromatin at some replication origins containing functional DNA-unwinding elements.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162384107.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 209209D-tyrosyl-tRNA(Tyr) deacylase 1PRO_0000164626Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei197 – 1971Phosphoserine3 Publications
Modified residuei205 – 2051Phosphoserine2 Publications

Post-translational modificationi

Preferentially phosphorylated in cells arrested early in S phase. Phosphorylation in the C-terminus weakens the interaction with CDC45.5 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8TEA8.
PaxDbiQ8TEA8.
PeptideAtlasiQ8TEA8.
PRIDEiQ8TEA8.

PTM databases

PhosphoSiteiQ8TEA8.

Expressioni

Tissue specificityi

Expressed in many adult and fetal tissues. Highest levels in testis, ovary, spleen and in adult and fetal brain.1 Publication

Gene expression databases

BgeeiQ8TEA8.
CleanExiHS_DTD1.
HS_HARS2.
GenevestigatoriQ8TEA8.

Organism-specific databases

HPAiHPA040981.
HPA042653.

Interactioni

Subunit structurei

Homodimer. Interacts with CDC45 and TOPBP1.2 Publications

Protein-protein interaction databases

BioGridi124965. 8 interactions.
IntActiQ8TEA8. 1 interaction.
STRINGi9606.ENSP00000366672.

Structurei

Secondary structure

1
209
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 1514Combined sources
Beta strandi18 – 3215Combined sources
Helixi39 – 5113Combined sources
Helixi67 – 704Combined sources
Beta strandi73 – 786Combined sources
Helixi80 – 823Combined sources
Beta strandi87 – 904Combined sources
Helixi99 – 11618Combined sources
Helixi119 – 1213Combined sources
Beta strandi122 – 1243Combined sources
Beta strandi131 – 14616Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2OKVX-ray2.00A/B/C/D1-209[»]
ProteinModelPortaliQ8TEA8.
SMRiQ8TEA8. Positions 1-150.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8TEA8.

Family & Domainsi

Sequence similaritiesi

Belongs to the DTD family.Curated

Phylogenomic databases

eggNOGiCOG1490.
GeneTreeiENSGT00390000008298.
HOVERGENiHBG039436.
InParanoidiQ8TEA8.
KOiK07560.
OMAiGDENDKM.
OrthoDBiEOG7SR4P0.
PhylomeDBiQ8TEA8.
TreeFamiTF314886.

Family and domain databases

Gene3Di3.50.80.10. 1 hit.
HAMAPiMF_00518. Deacylase_Dtd.
InterProiIPR023509. DTD-like_dom.
IPR003732. DTyrtRNA_deacyls.
[Graphical view]
PANTHERiPTHR10472. PTHR10472. 1 hit.
PfamiPF02580. Tyr_Deacylase. 1 hit.
[Graphical view]
SUPFAMiSSF69500. SSF69500. 1 hit.
TIGRFAMsiTIGR00256. TIGR00256. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8TEA8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKAVVQRVTR ASVTVGGEQI SAIGRGICVL LGISLEDTQK ELEHMVRKIL
60 70 80 90 100
NLRVFEDESG KHWSKSVMDK QYEILCVSQF TLQCVLKGNK PDFHLAMPTE
110 120 130 140 150
QAEGFYNSFL EQLRKTYRPE LIKDGKFGAY MQVHIQNDGP VTIELESPAP
160 170 180 190 200
GTATSDPKQL SKLEKQQQRK EKTRAKGPSE SSKERNTPRK EDRSASSGAE

GDVSSEREP
Length:209
Mass (Da):23,424
Last modified:March 28, 2003 - v2
Checksum:iF006ED14974ACC92
GO

Sequence cautioni

The sequence BAB85044.1 differs from that shown. Reason: Presence of Alu-repeat DNA.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti94 – 941H → N in AAH45167. (PubMed:15489334)Curated
Sequence conflicti134 – 1341H → R in AAL57046. (PubMed:12392168)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF332356 mRNA. Translation: AAL57046.1.
AK074304 mRNA. Translation: BAB85044.1. Sequence problems.
AK291440 mRNA. Translation: BAF84129.1.
AL121900, AL121780 Genomic DNA. Translation: CAH73147.1.
AL121780, AL121900 Genomic DNA. Translation: CAI15669.1.
CH471133 Genomic DNA. Translation: EAX10227.1.
BC000599 mRNA. No translation available.
CH471133 Genomic DNA. Translation: EAX10228.1.
BC045167 mRNA. Translation: AAH45167.1.
BC100923 mRNA. Translation: AAI00924.1.
BC100924 mRNA. Translation: AAI00925.1.
BC100925 mRNA. Translation: AAI00926.1.
CCDSiCCDS13138.1.
RefSeqiNP_543010.3. NM_080820.4.
UniGeneiHs.659442.

Genome annotation databases

EnsembliENST00000377452; ENSP00000366672; ENSG00000125821.
GeneIDi92675.
KEGGihsa:92675.
UCSCiuc002wrf.4. human.

Polymorphism databases

DMDMi29427856.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF332356 mRNA. Translation: AAL57046.1 .
AK074304 mRNA. Translation: BAB85044.1 . Sequence problems.
AK291440 mRNA. Translation: BAF84129.1 .
AL121900 , AL121780 Genomic DNA. Translation: CAH73147.1 .
AL121780 , AL121900 Genomic DNA. Translation: CAI15669.1 .
CH471133 Genomic DNA. Translation: EAX10227.1 .
BC000599 mRNA. No translation available.
CH471133 Genomic DNA. Translation: EAX10228.1 .
BC045167 mRNA. Translation: AAH45167.1 .
BC100923 mRNA. Translation: AAI00924.1 .
BC100924 mRNA. Translation: AAI00925.1 .
BC100925 mRNA. Translation: AAI00926.1 .
CCDSi CCDS13138.1.
RefSeqi NP_543010.3. NM_080820.4.
UniGenei Hs.659442.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2OKV X-ray 2.00 A/B/C/D 1-209 [» ]
ProteinModelPortali Q8TEA8.
SMRi Q8TEA8. Positions 1-150.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 124965. 8 interactions.
IntActi Q8TEA8. 1 interaction.
STRINGi 9606.ENSP00000366672.

PTM databases

PhosphoSitei Q8TEA8.

Polymorphism databases

DMDMi 29427856.

Proteomic databases

MaxQBi Q8TEA8.
PaxDbi Q8TEA8.
PeptideAtlasi Q8TEA8.
PRIDEi Q8TEA8.

Protocols and materials databases

DNASUi 92675.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000377452 ; ENSP00000366672 ; ENSG00000125821 .
GeneIDi 92675.
KEGGi hsa:92675.
UCSCi uc002wrf.4. human.

Organism-specific databases

CTDi 92675.
GeneCardsi GC20P018568.
HGNCi HGNC:16219. DTD1.
HPAi HPA040981.
HPA042653.
MIMi 610996. gene.
neXtProti NX_Q8TEA8.
PharmGKBi PA162384107.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG1490.
GeneTreei ENSGT00390000008298.
HOVERGENi HBG039436.
InParanoidi Q8TEA8.
KOi K07560.
OMAi GDENDKM.
OrthoDBi EOG7SR4P0.
PhylomeDBi Q8TEA8.
TreeFami TF314886.

Miscellaneous databases

ChiTaRSi DTD1. human.
EvolutionaryTracei Q8TEA8.
GenomeRNAii 92675.
NextBioi 77831.
PROi Q8TEA8.
SOURCEi Search...

Gene expression databases

Bgeei Q8TEA8.
CleanExi HS_DTD1.
HS_HARS2.
Genevestigatori Q8TEA8.

Family and domain databases

Gene3Di 3.50.80.10. 1 hit.
HAMAPi MF_00518. Deacylase_Dtd.
InterProi IPR023509. DTD-like_dom.
IPR003732. DTyrtRNA_deacyls.
[Graphical view ]
PANTHERi PTHR10472. PTHR10472. 1 hit.
Pfami PF02580. Tyr_Deacylase. 1 hit.
[Graphical view ]
SUPFAMi SSF69500. SSF69500. 1 hit.
TIGRFAMsi TIGR00256. TIGR00256. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and identification of a novel cDNA which may be associated with FKBP25."
    Meng X.X., Chen J.J., Yang Q.Q., Wang S., Chao Y., Ying K., Xie Y., Mao Y.
    Biochem. Genet. 40:303-310(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Fetal brain.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain and Hepatoma.
  3. "The DNA sequence and comparative analysis of human chromosome 20."
    Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E.
    , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
    Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain and Skin.
  6. "The c-myc DNA-unwinding element-binding protein modulates the assembly of DNA replication complexes in vitro."
    Casper J.M., Kemp M.G., Ghosh M., Randall G.M., Vaillant A., Leffak M.
    J. Biol. Chem. 280:13071-13083(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION, SUBCELLULAR LOCATION.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Platelet.
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-197 AND SER-205, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-197 AND SER-205, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  11. "The DNA unwinding element binding protein DUE-B interacts with Cdc45 in preinitiation complex formation."
    Chowdhury A., Liu G., Kemp M., Chen X., Katrangi N., Myers S., Ghosh M., Yao J., Gao Y., Bubulya P., Leffak M.
    Mol. Cell. Biol. 30:1495-1507(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION, INTERACTION WITH CDC45 AND TOPBP1.
  12. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  13. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  14. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-197, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  15. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  16. "Structure and function of the c-myc DNA-unwinding element-binding protein DUE-B."
    Kemp M., Bae B., Yu J.P., Ghosh M., Leffak M., Nair S.K.
    J. Biol. Chem. 282:10441-10448(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS), SUBUNIT, MAGNESIUM-BINDING SITES.

Entry informationi

Entry nameiDTD1_HUMAN
AccessioniPrimary (citable) accession number: Q8TEA8
Secondary accession number(s): A8K5X5
, D3DW37, Q496D1, Q5W184, Q8WXU8, Q9BW67, Q9H464, Q9H474
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: March 28, 2003
Last modified: November 26, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3