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Q8TE99

- PXYP1_HUMAN

UniProt

Q8TE99 - PXYP1_HUMAN

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Protein

2-phosphoxylose phosphatase 1

Gene

PXYLP1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Responsible for the 2-O-dephosphorylation of xylose in the glycosaminoglycan-protein linkage region of proteoglycans thereby regulating the amount of mature glycosaminoglycan (GAG) chains. Sulfated glycosaminoglycans (GAGs), including heparan sulfate and chondroitin sulfate, are synthesized on the so-called common GAG-protein linkage region (GlcUAbeta1-3Galbeta1-3Galbeta1-4Xylbeta1-O-Ser) of core proteins, which is formed by the stepwise addition of monosaccharide residues by the respective specific glycosyltransferases. Xylose 2-O-dephosphorylation during completion of linkage region formation is a prerequisite for the initiation and efficient elongation of the repeating disaccharide region of GAG chains.1 Publication

pH dependencei

Optimum pH is 5.81 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei97 – 971NucleophileBy similarity
Active sitei379 – 3791Proton donorBy similarity

GO - Molecular functioni

  1. acid phosphatase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
2-phosphoxylose phosphatase 11 Publication (EC:3.1.3.-1 Publication)
Alternative name(s):
Acid phosphatase-like protein 2
Xylosyl phosphataseImported1 Publication
epididymis luminal protein 124Imported1 Publication
Gene namesi
Name:PXYLP1Imported
Synonyms:ACPL2, HEL124Imported1 Publication, XYLP1 Publication
ORF Names:UNQ370/PRO706
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 3

Organism-specific databases

HGNCiHGNC:26303. PXYLP1.

Subcellular locationi

Golgi apparatus membrane 1 Publication; Single-pass type II membrane protein Sequence Analysis
Note: Colocalizes to Golgi apparatus in a B3GAT3-dependent manner.1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66CytoplasmicSequence Analysis
Transmembranei7 – 2721Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini28 – 480453LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134964387.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4804802-phosphoxylose phosphatase 1PRO_0000314924Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi305 – 3051N-linked (GlcNAc...)Sequence Analysis
Glycosylationi354 – 3541N-linked (GlcNAc...)PROSITE-ProRule annotation

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ8TE99.
PRIDEiQ8TE99.

PTM databases

PhosphoSiteiQ8TE99.

Expressioni

Tissue specificityi

Widely expressed. Strongly expressed in spleen, fetal liver, moderately in placenta, pancreas, kidney, thymus and colon.1 Publication

Gene expression databases

BgeeiQ8TE99.
CleanExiHS_ACPL2.
ExpressionAtlasiQ8TE99. baseline and differential.
GenevestigatoriQ8TE99.

Organism-specific databases

HPAiHPA017243.

Interactioni

Subunit structurei

Interacts with B3GAT3; the interaction increases the 2-phosphoxylose phosphatase activity of PXYLP1 during completion of linkage region formation in a B3GAT3-mediated manner.1 Publication

Protein-protein interaction databases

BioGridi124942. 3 interactions.
STRINGi9606.ENSP00000286353.

Structurei

3D structure databases

ProteinModelPortaliQ8TE99.
SMRiQ8TE99. Positions 86-450.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the histidine acid phosphatase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG321954.
HOGENOMiHOG000033787.
HOVERGENiHBG106056.
InParanoidiQ8TE99.
OMAiCHNVSFP.
PhylomeDBiQ8TE99.
TreeFamiTF318821.

Family and domain databases

Gene3Di3.40.50.1240. 2 hits.
InterProiIPR000560. His_Pase_superF_clade-2.
IPR029033. His_PPase_superfam.
[Graphical view]
PfamiPF00328. His_Phos_2. 1 hit.
[Graphical view]
SUPFAMiSSF53254. SSF53254. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8TE99-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLFRNRFLLL LALAALLAFV SLSLQFFHLI PVSTPKNGMS SKSRKRIMPD
60 70 80 90 100
PVTEPPVTDP VYEALLYCNI PSVAERSMEG HAPHHFKLVS VHVFIRHGDR
110 120 130 140 150
YPLYVIPKTK RPEIDCTLVA NRKPYHPKLE AFISHMSKGS GASFESPLNS
160 170 180 190 200
LPLYPNHPLC EMGELTQTGV VQHLQNGQLL RDIYLKKHKL LPNDWSADQL
210 220 230 240 250
YLETTGKSRT LQSGLALLYG FLPDFDWKKI YFRHQPSALF CSGSCYCPVR
260 270 280 290 300
NQYLEKEQRR QYLLRLKNSQ LEKTYGEMAK IVDVPTKQLR AANPIDSMLC
310 320 330 340 350
HFCHNVSFPC TRNGCVDMEH FKVIKTHQIE DERERREKKL YFGYSLLGAH
360 370 380 390 400
PILNQTIGRM QRATEGRKEE LFALYSAHDV TLSPVLSALG LSEARFPRFA
410 420 430 440 450
ARLIFELWQD REKPSEHSVR ILYNGVDVTF HTSFCQDHHK RSPKPMCPLE
460 470 480
NLVRFVKRDM FVALGGSGTN YYDACHREGF
Length:480
Mass (Da):55,240
Last modified:June 1, 2002 - v1
Checksum:iC1A8BEC2B68ECA62
GO
Isoform 2 (identifier: Q8TE99-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-27: MLFRNRFLLLLALAALLAFVSLSLQFF → MEHSVCPSSAV

Note: No experimental confirmation available.

Show »
Length:464
Mass (Da):53,257
Checksum:i334FF1A12565DADA
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2727MLFRN…SLQFF → MEHSVCPSSAV in isoform 2. 1 PublicationVSP_030432Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB827640 mRNA. Translation: BAO45795.1.
EU794677 mRNA. Translation: ACJ13731.1.
AY358460 mRNA. Translation: AAQ88825.1.
AK074331 mRNA. Translation: BAB85053.1.
CH471052 Genomic DNA. Translation: EAW79007.1.
CH471052 Genomic DNA. Translation: EAW79009.1.
BC035834 mRNA. Translation: AAH35834.1.
BC036701 mRNA. Translation: AAH36701.1.
CCDSiCCDS3116.1. [Q8TE99-1]
RefSeqiNP_001032249.1. NM_001037172.2. [Q8TE99-1]
NP_001269657.1. NM_001282728.1.
NP_689495.1. NM_152282.4. [Q8TE99-1]
UniGeneiHs.657887.

Genome annotation databases

EnsembliENST00000286353; ENSP00000286353; ENSG00000155893. [Q8TE99-1]
ENST00000393010; ENSP00000376733; ENSG00000155893. [Q8TE99-1]
GeneIDi92370.
KEGGihsa:92370.
UCSCiuc003etu.3. human. [Q8TE99-1]

Polymorphism databases

DMDMi74730610.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB827640 mRNA. Translation: BAO45795.1 .
EU794677 mRNA. Translation: ACJ13731.1 .
AY358460 mRNA. Translation: AAQ88825.1 .
AK074331 mRNA. Translation: BAB85053.1 .
CH471052 Genomic DNA. Translation: EAW79007.1 .
CH471052 Genomic DNA. Translation: EAW79009.1 .
BC035834 mRNA. Translation: AAH35834.1 .
BC036701 mRNA. Translation: AAH36701.1 .
CCDSi CCDS3116.1. [Q8TE99-1 ]
RefSeqi NP_001032249.1. NM_001037172.2. [Q8TE99-1 ]
NP_001269657.1. NM_001282728.1.
NP_689495.1. NM_152282.4. [Q8TE99-1 ]
UniGenei Hs.657887.

3D structure databases

ProteinModelPortali Q8TE99.
SMRi Q8TE99. Positions 86-450.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 124942. 3 interactions.
STRINGi 9606.ENSP00000286353.

PTM databases

PhosphoSitei Q8TE99.

Polymorphism databases

DMDMi 74730610.

Proteomic databases

PaxDbi Q8TE99.
PRIDEi Q8TE99.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000286353 ; ENSP00000286353 ; ENSG00000155893 . [Q8TE99-1 ]
ENST00000393010 ; ENSP00000376733 ; ENSG00000155893 . [Q8TE99-1 ]
GeneIDi 92370.
KEGGi hsa:92370.
UCSCi uc003etu.3. human. [Q8TE99-1 ]

Organism-specific databases

CTDi 92370.
GeneCardsi GC03P140950.
HGNCi HGNC:26303. PXYLP1.
HPAi HPA017243.
neXtProti NX_Q8TE99.
PharmGKBi PA134964387.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG321954.
HOGENOMi HOG000033787.
HOVERGENi HBG106056.
InParanoidi Q8TE99.
OMAi CHNVSFP.
PhylomeDBi Q8TE99.
TreeFami TF318821.

Miscellaneous databases

GenomeRNAii 92370.
NextBioi 77736.
PROi Q8TE99.

Gene expression databases

Bgeei Q8TE99.
CleanExi HS_ACPL2.
ExpressionAtlasi Q8TE99. baseline and differential.
Genevestigatori Q8TE99.

Family and domain databases

Gene3Di 3.40.50.1240. 2 hits.
InterProi IPR000560. His_Pase_superF_clade-2.
IPR029033. His_PPase_superfam.
[Graphical view ]
Pfami PF00328. His_Phos_2. 1 hit.
[Graphical view ]
SUPFAMi SSF53254. SSF53254. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of phosphatase that dephosphorylates xylose in the glycosaminoglycan-protein linkage region of proteoglycans."
    Koike T., Izumikawa T., Sato B., Kitagawa H.
    J. Biol. Chem. 289:6695-6708(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, INTERACTION WITH B3GAT3, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: OvaryImported.
  2. Li J.Y., Wang H.Y., Liu F.J., Liu J.
    Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Hepatoma.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Ovary and Testis.

Entry informationi

Entry nameiPXYP1_HUMAN
AccessioniPrimary (citable) accession number: Q8TE99
Secondary accession number(s): D3DNF5, Q49AJ2, W0TR04
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 1, 2002
Last modified: November 26, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3