Q8TE77 (SSH3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein phosphatase Slingshot homolog 3
EC=3.1.3.16
EC=3.1.3.48

Alternative name(s):
SSH-like protein 3
Short name=SSH-3L
Short name=hSSH-3L

Gene names

Name:	SSH3
Synonyms:	SSH3L

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	659 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Protein phosphatase which may play a role in the regulation of actin filament dynamics. Can dephosphorylate and activate the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly By similarity.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. A phosphoprotein + H2O = a protein + phosphate.
Subunit structure	Does not bind to, or colocalize with, filamentous actin By similarity.
Subcellular location	Cytoplasm › cytoskeleton By similarity. Nucleus By similarity.
Miscellaneous	Tyrosine phosphatase activity has not been demonstrated for this protein to date.
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Molecular function	Hydrolase Protein phosphatase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	peptidyl-tyrosine dephosphorylation Inferred from electronic annotation. Source: GOC regulation of actin polymerization or depolymerization Inferred from Biological aspect of Ancestor. Source: RefGenome regulation of axonogenesis Inferred from Biological aspect of Ancestor. Source: RefGenome regulation of lamellipodium assembly Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	cytoplasm Inferred from Biological aspect of Ancestor. Source: RefGenome cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro actin binding Inferred from Biological aspect of Ancestor. Source: RefGenome protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC protein tyrosine/serine/threonine phosphatase activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8TE77-1)

Also known as: L;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8TE77-2)

The sequence of this isoform differs from the canonical sequence as follows:
     470-471: SR → RT
     472-659: Missing.

Isoform 3 (identifier: Q8TE77-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-146: Missing.
     147-154: LGVDFPDS → MAFPLSPA

Isoform 4 (identifier: Q8TE77-4)

The sequence of this isoform differs from the canonical sequence as follows:
     283-547: Missing.

Note: No experimental confirmation available.

Isoform 5 (identifier: Q8TE77-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-330: Missing.
     331-360: RIFPHLYLGSEWNAANLEELQRNRVTHILN → MEGTMMMQQRPVLSQQHPSFILNSSPAHSP
     470-471: SR → RT
     472-659: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 659	659	Protein phosphatase Slingshot homolog 3		PRO_0000094845
Regions
	Domain	328 – 468	141	Tyrosine-protein phosphatase
Sites
	Active site	413	1	Phosphocysteine intermediate By similarity
Amino acid modifications
	Modified residue	7	1	Phosphoserine By similarity
	Modified residue	9	1	Phosphoserine By similarity
	Modified residue	37	1	Phosphoserine By similarity
	Modified residue	85	1	Phosphoserine Ref.5
	Modified residue	87	1	Phosphoserine Ref.5
	Modified residue	649	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	1 – 330	330	Missing in isoform 5.		VSP_016330
	Alternative sequence	1 – 146	146	Missing in isoform 3.		VSP_016331
	Alternative sequence	147 – 154	8	LGVDFPDS → MAFPLSPA in isoform 3.		VSP_016332
	Alternative sequence	283 – 547	265	Missing in isoform 4.		VSP_016333
	Alternative sequence	331 – 360	30	RIFPH…THILN → MEGTMMMQQRPVLSQQHPSF ILNSSPAHSP in isoform 5.		VSP_016334
	Alternative sequence	470 – 471	2	SR → RT in isoform 2 and isoform 5.		VSP_016335
	Alternative sequence	472 – 659	188	Missing in isoform 2 and isoform 5.		VSP_016336
	Natural variant	239	1	E → V. Corresponds to variant rs7114712 [ dbSNP | Ensembl ].		VAR_057132
	Natural variant	600	1	R → H. Corresponds to variant rs1573536 [ dbSNP | Ensembl ].		VAR_057133
Experimental info
	Sequence conflict	58	1	A → V Ref.1
	Sequence conflict	58	1	A → V Ref.2
	Sequence conflict	509	1	E → G in BAC04314. Ref.3
	Sequence conflict	641	1	F → S in BAB85080. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (L) [UniParc]. Last modified November 22, 2005. Version 2. Checksum: 0D96F86EAFE81D3B	FASTA	659	72,996
	10 20 30 40 50 60 MALVTVSRSP PGSGASTPVG PWDQAVQRRS RLQRRQSFAV LRGAVLGLQD GGDNDDAAEA 70 80 90 100 110 120 SSEPTEKAPS EEELHGDQTD FGQGSQSPQK QEEQRQHLHL MVQLLRPQDD IRLAAQLEAP 130 140 150 160 170 180 RPPRLRYLLV VSTREGEGLS QDETVLLGVD FPDSSSPSCT LGLVLPLWSD TQVYLDGDGG 190 200 210 220 230 240 FSVTSGGQSR IFKPISIQTM WATLQVLHQA CEAALGSGLV PGGSALTWAS HYQERLNSEQ 250 260 270 280 290 300 SCLNEWTAMA DLESLRPPSA EPGGSSEQEQ MEQAIRAELW KVLDVSDLES VTSKEIRQAL 310 320 330 340 350 360 ELRLGLPLQQ YRDFIDNQML LLVAQRDRAS RIFPHLYLGS EWNAANLEEL QRNRVTHILN 370 380 390 400 410 420 MAREIDNFYP ERFTYHNVRL WDEESAQLLP HWKETHRFIE AARAQGTHVL VHCKMGVSRS 430 440 450 460 470 480 AATVLAYAMK QYECSLEQAL RHVQELRPIA RPNPGFLRQL QIYQGILTAS RQSHVWEQKV 490 500 510 520 530 540 GGVSPEEHPA PEVSTPFPPL PPEPEGGGEE KVVGMEESQA APKEEPGPRP RINLRGVMRS 550 560 570 580 590 600 ISLLEPSLEL ESTSETSDMP EVFSSHESSH EEPLQPFPQL ARTKGGQQVD RGPQPALKSR 610 620 630 640 650 QSVVTLQGSA VVANRTQAFQ EQEQGQGQGQ GEPCISSTPR FRKVVRQASV HDSGEEGEA
	Isoform 2 [UniParc]. Checksum: 826A8914B638DCC0	FASTA	471	52,713
	10 20 30 40 50 60 MALVTVSRSP PGSGASTPVG PWDQAVQRRS RLQRRQSFAV LRGAVLGLQD GGDNDDAAEA 70 80 90 100 110 120 SSEPTEKAPS EEELHGDQTD FGQGSQSPQK QEEQRQHLHL MVQLLRPQDD IRLAAQLEAP 130 140 150 160 170 180 RPPRLRYLLV VSTREGEGLS QDETVLLGVD FPDSSSPSCT LGLVLPLWSD TQVYLDGDGG 190 200 210 220 230 240 FSVTSGGQSR IFKPISIQTM WATLQVLHQA CEAALGSGLV PGGSALTWAS HYQERLNSEQ 250 260 270 280 290 300 SCLNEWTAMA DLESLRPPSA EPGGSSEQEQ MEQAIRAELW KVLDVSDLES VTSKEIRQAL 310 320 330 340 350 360 ELRLGLPLQQ YRDFIDNQML LLVAQRDRAS RIFPHLYLGS EWNAANLEEL QRNRVTHILN 370 380 390 400 410 420 MAREIDNFYP ERFTYHNVRL WDEESAQLLP HWKETHRFIE AARAQGTHVL VHCKMGVSRS 430 440 450 460 470 AATVLAYAMK QYECSLEQAL RHVQELRPIA RPNPGFLRQL QIYQGILTAR T
	Isoform 3 [UniParc]. Checksum: CA5FF8403E02B99F	FASTA	513	56,982
	10 20 30 40 50 60 MAFPLSPASS PSCTLGLVLP LWSDTQVYLD GDGGFSVTSG GQSRIFKPIS IQTMWATLQV 70 80 90 100 110 120 LHQACEAALG SGLVPGGSAL TWASHYQERL NSEQSCLNEW TAMADLESLR PPSAEPGGSS 130 140 150 160 170 180 EQEQMEQAIR AELWKVLDVS DLESVTSKEI RQALELRLGL PLQQYRDFID NQMLLLVAQR 190 200 210 220 230 240 DRASRIFPHL YLGSEWNAAN LEELQRNRVT HILNMAREID NFYPERFTYH NVRLWDEESA 250 260 270 280 290 300 QLLPHWKETH RFIEAARAQG THVLVHCKMG VSRSAATVLA YAMKQYECSL EQALRHVQEL 310 320 330 340 350 360 RPIARPNPGF LRQLQIYQGI LTASRQSHVW EQKVGGVSPE EHPAPEVSTP FPPLPPEPEG 370 380 390 400 410 420 GGEEKVVGME ESQAAPKEEP GPRPRINLRG VMRSISLLEP SLELESTSET SDMPEVFSSH 430 440 450 460 470 480 ESSHEEPLQP FPQLARTKGG QQVDRGPQPA LKSRQSVVTL QGSAVVANRT QAFQEQEQGQ 490 500 510 GQGQGEPCIS STPRFRKVVR QASVHDSGEE GEA
	Isoform 4 [UniParc]. Checksum: F87C7F5A29048AAE	FASTA	394	42,724
	10 20 30 40 50 60 MALVTVSRSP PGSGASTPVG PWDQAVQRRS RLQRRQSFAV LRGAVLGLQD GGDNDDAAEA 70 80 90 100 110 120 SSEPTEKAPS EEELHGDQTD FGQGSQSPQK QEEQRQHLHL MVQLLRPQDD IRLAAQLEAP 130 140 150 160 170 180 RPPRLRYLLV VSTREGEGLS QDETVLLGVD FPDSSSPSCT LGLVLPLWSD TQVYLDGDGG 190 200 210 220 230 240 FSVTSGGQSR IFKPISIQTM WATLQVLHQA CEAALGSGLV PGGSALTWAS HYQERLNSEQ 250 260 270 280 290 300 SCLNEWTAMA DLESLRPPSA EPGGSSEQEQ MEQAIRAELW KVLELESTSE TSDMPEVFSS 310 320 330 340 350 360 HESSHEEPLQ PFPQLARTKG GQQVDRGPQP ALKSRQSVVT LQGSAVVANR TQAFQEQEQG 370 380 390 QGQGQGEPCI SSTPRFRKVV RQASVHDSGE EGEA
	Isoform 5 [UniParc]. Checksum: 5B268790931CA5B6	FASTA	141	16,357
	10 20 30 40 50 60 MEGTMMMQQR PVLSQQHPSF ILNSSPAHSP MAREIDNFYP ERFTYHNVRL WDEESAQLLP 70 80 90 100 110 120 HWKETHRFIE AARAQGTHVL VHCKMGVSRS AATVLAYAMK QYECSLEQAL RHVQELRPIA 130 140 RPNPGFLRQL QIYQGILTAR T

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Control of actin reorganization by Slingshot, a family of phosphatases that dephosphorylate ADF/cofilin." Niwa R., Nagata-Ohashi K., Takeichi M., Mizuno K., Uemura T. Cell 108:233-246(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]	"Differential activities, subcellular distribution and tissue expression patterns of three members of Slingshot family phosphatases that dephosphorylate cofilin." Ohta Y., Kousaka K., Nagata-Ohashi K., Ohashi K., Muramoto A., Shima Y., Niwa R., Uemura T., Mizuno K. Genes Cells 8:811-824(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 3; 4 AND 5). Tissue: Cerebellum and Ovarian carcinoma.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Uterus.
[5]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-85 AND SER-87, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[6]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB072360 mRNA. Translation: BAB84119.3. AB099291 mRNA. Translation: BAC97814.1. AK000522 mRNA. Translation: BAA91228.1. AK001790 mRNA. Translation: BAA91913.1. AK074432 mRNA. Translation: BAB85080.1. AK094226 mRNA. Translation: BAC04314.1. BC007709 mRNA. Translation: AAH07709.1.
IPI	IPI00016485. IPI00171057. IPI00218486. IPI00385839. IPI00655638.
RefSeq	NP_060327.3. NM_017857.3.
UniGene	Hs.29173.
3D structure databases
ProteinModelPortal	Q8TE77.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000312081.
PTM databases
PhosphoSite	Q8TE77.
Polymorphism databases
DMDM	82582268.
Proteomic databases
PaxDb	Q8TE77.
PRIDE	Q8TE77.
Protocols and materials databases
DNASU	54961.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000308127; ENSP00000312081; ENSG00000172830. ENST00000376757; ENSP00000365948; ENSG00000172830.
GeneID	54961.
KEGG	hsa:54961.
UCSC	uc001okj.3. human. uc001okl.3. human.
Organism-specific databases
CTD	54961.
GeneCards	GC11P067071.
HGNC	HGNC:30581. SSH3.
HPA	HPA019949. HPA019957.
MIM	606780. gene.
neXtProt	NX_Q8TE77.
PharmGKB	PA134929326.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG2453.
HOVERGEN	HBG089321.
InParanoid	Q8TE77.
KO	K05766.
OMA	HILNMAR.
OrthoDB	EOG4WDDBT.
PhylomeDB	Q8TE77.
Gene expression databases
ArrayExpress	Q8TE77.
Bgee	Q8TE77.
CleanEx	HS_SSH3.
Genevestigator	Q8TE77.
GermOnline	ENSG00000172830. Homo sapiens.
Family and domain databases
Gene3D	1.10.10.60. 1 hit.
InterPro	IPR014876. DEK_C. IPR000340. Dual-sp_phosphatase_cat-dom. IPR020422. Dual-sp_phosphatase_subgr_cat. IPR024950. DUSP. IPR009057. Homeodomain-like. IPR000387. Tyr/Dual-sp_Pase. IPR016130. Tyr_Pase_AS. [Graphical view]
PANTHER	PTHR10159. PTHR10159. 1 hit.
Pfam	PF08766. DEK_C. 1 hit. PF00782. DSPc. 1 hit. [Graphical view]
SMART	SM00195. DSPc. 1 hit. [Graphical view]
PROSITE	PS00383. TYR_PHOSPHATASE_1. 1 hit. PS50056. TYR_PHOSPHATASE_2. 1 hit. PS50054. TYR_PHOSPHATASE_DUAL. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	54961.
NextBio	58168.
SOURCE	Search...

Entry information

Entry name

SSH3_HUMAN

Accession

Primary (citable) accession number: Q8TE77
Secondary accession number(s): Q6PK42 , Q76I75, Q8N9L8, Q8WYL0, Q9NV45, Q9NWZ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	November 22, 2005
Last modified:	May 1, 2013
This is version 96 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.