ID   EOLA1_HUMAN             Reviewed;         158 AA.
AC   Q8TE69; A0A0A0MTB5; A8K784; B7Z6H6; B7ZL96; D6RA72; E7ENU3; Q2M3E9;
DT   21-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT   28-MAR-2018, sequence version 3.
DT   24-JAN-2024, entry version 147.
DE   RecName: Full=Protein EOLA1 {ECO:0000305};
DE   AltName: Full=Endothelial-overexpressed lipopolysaccharide-associated factor 1 {ECO:0000303|PubMed:15541360};
DE   AltName: Full=Endothelium and lymphocyte associated ASCH domain 1 {ECO:0000312|HGNC:HGNC:28089};
GN   Name=EOLA1 {ECO:0000312|HGNC:HGNC:28089}; Synonyms=CXorf40, CXorf40A;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX   PubMed=8717057; DOI=10.1101/gr.5.1.71;
RA   Timms K.M., Lu F., Shen Y., Pierson C.A., Muzny D.M., Gu Y., Nelson D.L.,
RA   Gibbs R.A.;
RT   "130 kb of DNA sequence reveals two new genes and a regional duplication
RT   distal to the human iduronate-2-sulfate sulfatase locus.";
RL   Genome Res. 5:71-78(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, AND INTERACTION
RP   WITH MT2A.
RX   PubMed=15541360; DOI=10.1016/j.bbrc.2004.10.124;
RA   Liang Z., Yang Z.;
RT   "Identification and characterization of a novel gene EOLA1 stimulating
RT   ECV304 cell proliferation.";
RL   Biochem. Biophys. Res. Commun. 325:798-802(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC   TISSUE=Placenta, and Skeletal muscle;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15772651; DOI=10.1038/nature03440;
RA   Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D.,
RA   Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L.,
RA   Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C.,
RA   Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A.,
RA   Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P.,
RA   Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D.,
RA   Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D.,
RA   Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L.,
RA   Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P.,
RA   Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G.,
RA   Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J.,
RA   Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D.,
RA   Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L.,
RA   Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z.,
RA   Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S.,
RA   Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S.,
RA   Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O.,
RA   Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H.,
RA   Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T.,
RA   Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L.,
RA   Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R.,
RA   Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y.,
RA   Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K.,
RA   Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J.,
RA   Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L.,
RA   Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S.,
RA   Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A.,
RA   Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L.,
RA   Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D.,
RA   Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H.,
RA   McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S.,
RA   Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C.,
RA   Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S.,
RA   Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V.,
RA   Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K.,
RA   Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K.,
RA   Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D.,
RA   Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R.,
RA   Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B.,
RA   Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C.,
RA   d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q.,
RA   Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N.,
RA   Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A.,
RA   Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J.,
RA   Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A.,
RA   Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F.,
RA   Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L.,
RA   Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S.,
RA   Rogers J., Bentley D.R.;
RT   "The DNA sequence of the human X chromosome.";
RL   Nature 434:325-337(2005).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Brain, and Eye;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [6]
RP   INDUCTION BY LPS, AND FUNCTION.
RX   PubMed=24916366; DOI=10.1007/s11010-014-2110-7;
RA   Liu Y., Liu H., Chen W., Yang T., Zhang W.;
RT   "EOLA1 protects lipopolysaccharide induced IL-6 production and apoptosis by
RT   regulation of MT2A in human umbilical vein endothelial cells.";
RL   Mol. Cell. Biochem. 395:45-51(2014).
RN   [7]
RP   TISSUE SPECIFICITY.
RX   PubMed=31007603; DOI=10.1155/2019/6705424;
RA   Wu M., Leng W., Pan H., Lei X., Chen L., Ouyang X., Liang Z.;
RT   "The Reduced Expression of EOLA1 May Be Related to Refractory Diabetic Foot
RT   Ulcer.";
RL   Mediators Inflamm. 2019:6705424-6705424(2019).
RN   [8] {ECO:0007744|PDB:5Y7D}
RP   X-RAY CRYSTALLOGRAPHY (1.71 ANGSTROMS).
RX   PubMed=31569543; DOI=10.3390/molecules24193529;
RA   Kim M., Park S.H., Park J.S., Kim H.J., Han B.W.;
RT   "Crystal Structure of Human EOLA1 Implies Its Possibility of RNA Binding.";
RL   Molecules 24:0-0(2019).
CC   -!- FUNCTION: May play a role in cell protection during the inflammatory
CC       response. In epithelial cells, negatively regulates IL6 production and
CC       apoptosis through the regulation of MT2A expression (PubMed:24916366).
CC       {ECO:0000269|PubMed:24916366}.
CC   -!- SUBUNIT: Interacts with MT2A. {ECO:0000269|PubMed:15541360}.
CC   -!- INTERACTION:
CC       Q8TE69; P02795: MT2A; NbExp=2; IntAct=EBI-996609, EBI-996616;
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q8TE69-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q8TE69-2; Sequence=VSP_047240;
CC   -!- TISSUE SPECIFICITY: Expressed primarily in heart, skeletal muscle,
CC       kidney, liver and placenta. Relatively high level of expression in
CC       spleen, colon and small intestine. Almost no expression in brain,
CC       thymus, lung and peripheral blood leukocytes. Expressed in epithelial
CC       cells (at protein level) (PubMed:31007603).
CC       {ECO:0000269|PubMed:15541360, ECO:0000269|PubMed:31007603}.
CC   -!- INDUCTION: Induced by LPS (at protein level).
CC       {ECO:0000269|PubMed:24916366}.
CC   -!- SIMILARITY: Belongs to the EOLA family. {ECO:0000305}.
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DR   EMBL; L43580; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; AY074889; AAL71889.1; -; mRNA.
DR   EMBL; AC244197; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AK291899; BAF84588.1; -; mRNA.
DR   EMBL; AK300334; BAH13262.1; -; mRNA.
DR   EMBL; BC065539; AAH65539.1; -; mRNA.
DR   EMBL; BC104930; AAI04931.1; -; mRNA.
DR   EMBL; BC104932; AAI04933.1; -; mRNA.
DR   EMBL; BC143660; AAI43661.1; -; mRNA.
DR   EMBL; BC143661; AAI43662.1; -; mRNA.
DR   EMBL; BC143662; AAI43663.1; -; mRNA.
DR   CCDS; CCDS14687.1; -. [Q8TE69-1]
DR   CCDS; CCDS55522.1; -. [Q8TE69-2]
DR   RefSeq; NP_001165378.1; NM_001171907.2. [Q8TE69-1]
DR   RefSeq; NP_001165379.1; NM_001171908.2. [Q8TE69-1]
DR   RefSeq; NP_001165380.1; NM_001171909.3. [Q8TE69-2]
DR   RefSeq; NP_001311203.1; NM_001324274.1. [Q8TE69-1]
DR   RefSeq; NP_001311204.1; NM_001324275.1. [Q8TE69-1]
DR   RefSeq; NP_001311205.1; NM_001324276.1. [Q8TE69-2]
DR   RefSeq; NP_001311206.1; NM_001324277.1. [Q8TE69-1]
DR   RefSeq; NP_001311207.1; NM_001324278.1. [Q8TE69-1]
DR   RefSeq; NP_001311208.1; NM_001324279.1. [Q8TE69-2]
DR   RefSeq; NP_001311209.1; NM_001324280.1. [Q8TE69-1]
DR   RefSeq; NP_835225.2; NM_178124.5. [Q8TE69-1]
DR   RefSeq; XP_005262406.2; XM_005262349.4.
DR   PDB; 5Y7D; X-ray; 1.71 A; A=1-158.
DR   PDBsum; 5Y7D; -.
DR   AlphaFoldDB; Q8TE69; -.
DR   SMR; Q8TE69; -.
DR   BioGRID; 124897; 6.
DR   IntAct; Q8TE69; 1.
DR   STRING; 9606.ENSP00000423099; -.
DR   BioMuta; CXorf40A; -.
DR   DMDM; 519668664; -.
DR   EPD; Q8TE69; -.
DR   MassIVE; Q8TE69; -.
DR   MaxQB; Q8TE69; -.
DR   PaxDb; 9606-ENSP00000423099; -.
DR   PeptideAtlas; Q8TE69; -.
DR   Pumba; Q8TE69; -.
DR   Antibodypedia; 55906; 58 antibodies from 11 providers.
DR   DNASU; 91966; -.
DR   Ensembl; ENST00000359293.5; ENSP00000420882.1; ENSG00000197620.11. [Q8TE69-1]
DR   Ensembl; ENST00000393985.8; ENSP00000421745.1; ENSG00000197620.11. [Q8TE69-1]
DR   Ensembl; ENST00000422892.2; ENSP00000422312.1; ENSG00000197620.11. [Q8TE69-2]
DR   Ensembl; ENST00000423421.5; ENSP00000422512.1; ENSG00000197620.11. [Q8TE69-1]
DR   Ensembl; ENST00000423540.6; ENSP00000425520.1; ENSG00000197620.11. [Q8TE69-1]
DR   Ensembl; ENST00000434353.6; ENSP00000423160.1; ENSG00000197620.11. [Q8TE69-2]
DR   Ensembl; ENST00000441248.5; ENSP00000423099.1; ENSG00000197620.11. [Q8TE69-1]
DR   Ensembl; ENST00000450602.6; ENSP00000427540.1; ENSG00000197620.11. [Q8TE69-1]
DR   Ensembl; ENST00000514208.5; ENSP00000423708.1; ENSG00000197620.11. [Q8TE69-2]
DR   GeneID; 91966; -.
DR   KEGG; hsa:91966; -.
DR   MANE-Select; ENST00000393985.8; ENSP00000421745.1; NM_001171907.3; NP_001165378.1.
DR   UCSC; uc022cgi.2; human.
DR   UCSC; uc033ezo.2; human. [Q8TE69-1]
DR   AGR; HGNC:28089; -.
DR   CTD; 91966; -.
DR   DisGeNET; 91966; -.
DR   GeneCards; EOLA1; -.
DR   HGNC; HGNC:28089; EOLA1.
DR   HPA; ENSG00000197620; Low tissue specificity.
DR   MIM; 300954; gene.
DR   neXtProt; NX_Q8TE69; -.
DR   OpenTargets; ENSG00000197620; -.
DR   VEuPathDB; HostDB:ENSG00000197620; -.
DR   eggNOG; ENOG502RZ9S; Eukaryota.
DR   GeneTree; ENSGT00390000012182; -.
DR   HOGENOM; CLU_116791_0_0_1; -.
DR   InParanoid; Q8TE69; -.
DR   OMA; HVAQRDW; -.
DR   OrthoDB; 5314616at2759; -.
DR   PhylomeDB; Q8TE69; -.
DR   TreeFam; TF332845; -.
DR   PathwayCommons; Q8TE69; -.
DR   SignaLink; Q8TE69; -.
DR   BioGRID-ORCS; 91966; 16 hits in 670 CRISPR screens.
DR   ChiTaRS; CXorf40A; human.
DR   GeneWiki; CXorf40A; -.
DR   GenomeRNAi; 91966; -.
DR   Pharos; Q8TE69; Tbio.
DR   PRO; PR:Q8TE69; -.
DR   Proteomes; UP000005640; Chromosome X.
DR   RNAct; Q8TE69; Protein.
DR   Bgee; ENSG00000197620; Expressed in apex of heart and 96 other cell types or tissues.
DR   ExpressionAtlas; Q8TE69; baseline and differential.
DR   GO; GO:0010468; P:regulation of gene expression; IDA:UniProtKB.
DR   GO; GO:0032675; P:regulation of interleukin-6 production; IDA:UniProtKB.
DR   InterPro; IPR007374; ASCH_domain.
DR   InterPro; IPR033615; EOLA1/EOLA2.
DR   InterPro; IPR015947; PUA-like_sf.
DR   PANTHER; PTHR31666; PROTEIN CXORF40A-RELATED; 1.
DR   PANTHER; PTHR31666:SF0; PROTEIN EOLA1-RELATED; 1.
DR   SMART; SM01022; ASCH; 1.
DR   SUPFAM; SSF88697; PUA domain-like; 1.
DR   Genevisible; Q8TE69; HS.
PE   1: Evidence at protein level;
KW   3D-structure; Alternative splicing; Reference proteome.
FT   CHAIN           1..158
FT                   /note="Protein EOLA1"
FT                   /id="PRO_0000079740"
FT   DOMAIN          6..92
FT                   /note="ASCH"
FT                   /evidence="ECO:0000255"
FT   VAR_SEQ         145..158
FT                   /note="VDIPEHLIPLGHEV -> NP (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:14702039"
FT                   /id="VSP_047240"
FT   CONFLICT        2
FT                   /note="K -> E (in Ref. 3; BAH13262)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        7
FT                   /note="S -> P (in Ref. 3; BAH13262)"
FT                   /evidence="ECO:0000305"
FT   STRAND          1..8
FT                   /evidence="ECO:0007829|PDB:5Y7D"
FT   HELIX           12..17
FT                   /evidence="ECO:0007829|PDB:5Y7D"
FT   STRAND          23..28
FT                   /evidence="ECO:0007829|PDB:5Y7D"
FT   HELIX           32..34
FT                   /evidence="ECO:0007829|PDB:5Y7D"
FT   STRAND          37..43
FT                   /evidence="ECO:0007829|PDB:5Y7D"
FT   HELIX           52..59
FT                   /evidence="ECO:0007829|PDB:5Y7D"
FT   HELIX           65..75
FT                   /evidence="ECO:0007829|PDB:5Y7D"
FT   TURN            76..78
FT                   /evidence="ECO:0007829|PDB:5Y7D"
FT   STRAND          80..94
FT                   /evidence="ECO:0007829|PDB:5Y7D"
FT   HELIX           101..111
FT                   /evidence="ECO:0007829|PDB:5Y7D"
FT   STRAND          120..135
FT                   /evidence="ECO:0007829|PDB:5Y7D"
FT   STRAND          140..147
FT                   /evidence="ECO:0007829|PDB:5Y7D"
FT   HELIX           149..151
FT                   /evidence="ECO:0007829|PDB:5Y7D"
SQ   SEQUENCE   158 AA;  17891 MW;  0C06424E1953F1B1 CRC64;
     MKFGCLSFRQ PYAGFVLNGI KTVETRWRPL LSSQRNCTIA VHIAHRDWEG DAWRELLVER
     LGMTPAQIQA LLRKGEKFGR GVIAGLVDIG ETLQCPEDLT PDEVVELENQ AVLTNLKQKY
     LTVISNPRWL LEPIPRKGGK DVFQVDIPEH LIPLGHEV
//