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Protein

Epidermal growth factor receptor kinase substrate 8-like protein 1

Gene

EPS8L1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Stimulates guanine exchange activity of SOS1. May play a role in membrane ruffling and remodeling of the actin cytoskeleton.1 Publication

GO - Molecular functioni

  • actin binding Source: UniProtKB
  • cadherin binding Source: BHF-UCL
  • T cell receptor binding Source: UniProtKB

GO - Biological processi

  • positive regulation of ruffle assembly Source: UniProtKB
  • regulation of Rho protein signal transduction Source: UniProtKB
  • Rho protein signal transduction Source: UniProtKB

Names & Taxonomyi

Protein namesi
Recommended name:
Epidermal growth factor receptor kinase substrate 8-like protein 1
Short name:
EPS8-like protein 1
Alternative name(s):
Epidermal growth factor receptor pathway substrate 8-related protein 1
Short name:
EPS8-related protein 1
Gene namesi
Name:EPS8L1
Synonyms:DRC3, EPS8R1
ORF Names:PP10566
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000131037.14
HGNCiHGNC:21295 EPS8L1
MIMi614987 gene
neXtProtiNX_Q8TE68

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000131037
PharmGKBiPA134990326

Polymorphism and mutation databases

BioMutaiEPS8L1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002390821 – 723Epidermal growth factor receptor kinase substrate 8-like protein 1Add BLAST723

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei182PhosphoserineCombined sources1
Modified residuei187PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8TE68
MaxQBiQ8TE68
PaxDbiQ8TE68
PeptideAtlasiQ8TE68
PRIDEiQ8TE68
ProteomicsDBi74407
74408 [Q8TE68-2]
74409 [Q8TE68-3]
74410 [Q8TE68-4]

PTM databases

iPTMnetiQ8TE68
PhosphoSitePlusiQ8TE68

Expressioni

Tissue specificityi

Detected in placenta.1 Publication

Gene expression databases

BgeeiENSG00000131037
CleanExiHS_EPS8L1
ExpressionAtlasiQ8TE68 baseline and differential
GenevisibleiQ8TE68 HS

Organism-specific databases

HPAiHPA041851
HPA043545

Interactioni

Subunit structurei

Interacts with ABI1. Part of a complex that contains SOS1, ABI1 and EPS8L2. Associates with F-actin.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • actin binding Source: UniProtKB
  • cadherin binding Source: BHF-UCL
  • T cell receptor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi120218, 10 interactors
CORUMiQ8TE68
IntActiQ8TE68, 4 interactors
MINTiQ8TE68

Structurei

Secondary structure

1723
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi482 – 487Combined sources6
Beta strandi493 – 496Combined sources4
Beta strandi504 – 509Combined sources6
Beta strandi511 – 518Combined sources8
Beta strandi525 – 528Combined sources4
Helixi529 – 531Combined sources3
Beta strandi532 – 534Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2K2MNMR-A481-536[»]
2ROLNMR-A478-537[»]
ProteinModelPortaliQ8TE68
SMRiQ8TE68
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8TE68

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini478 – 537SH3PROSITE-ProRule annotationAdd BLAST60

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili689 – 719Sequence analysisAdd BLAST31

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi119 – 122Poly-Leu4
Compositional biasi448 – 451Poly-Arg4
Compositional biasi534 – 576Pro-richAdd BLAST43

Sequence similaritiesi

Belongs to the EPS8 family.Curated

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiENOG410IP0J Eukaryota
ENOG4111F7Z LUCA
GeneTreeiENSGT00390000003646
HOVERGENiHBG003090
InParanoidiQ8TE68
KOiK17277
OMAiSQYPVNH
OrthoDBiEOG091G023T
PhylomeDBiQ8TE68
TreeFamiTF313069

Family and domain databases

CDDicd01210 PTB_EPS8, 1 hit
cd11764 SH3_Eps8, 1 hit
Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR033928 EPS8_PTB
IPR035462 Eps8_SH3
IPR011993 PH-like_dom_sf
IPR013625 PTB
IPR006020 PTB/PI_dom
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PfamiView protein in Pfam
PF08416 PTB, 1 hit
PF00018 SH3_1, 1 hit
SMARTiView protein in SMART
SM00462 PTB, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS50002 SH3, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8TE68-1) [UniParc]FASTAAdd to basket
Also known as: A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSTATGPEAA PKPSAKSIYE QRKRYSTVVM ADVSQYPVNH LVTFCLGEDD
60 70 80 90 100
GVHTVEDASR KLAVMDSQGR VWAQEMLLRV SPDHVTLLDP ASKEELESYP
110 120 130 140 150
LGAIVRCDAV MPPGRSRSLL LLVCQEPERA QPDVHFFQGL RLGAELIRED
160 170 180 190 200
IQGALHNYRS GRGERRAAAL RATQEELQRD RSPAAETPPL QRRPSVRAVI
210 220 230 240 250
STVERGAGRG RPQAKPIPEA EEAQRPEPVG TSSNADSASP DLGPRGPDLA
260 270 280 290 300
VLQAEREVDI LNHVFDDVES FVSRLQKSAE AARVLEHRER GRRSRRRAAG
310 320 330 340 350
EGLLTLRAKP PSEAEYTDVL QKIKYAFSLL ARLRGNIADP SSPELLHFLF
360 370 380 390 400
GPLQMIVNTS GGPEFASSVR RPHLTSDAVA LLRDNVTPRE NELWTSLGDS
410 420 430 440 450
WTRPGLELSP EEGPPYRPEF FSGWEPPVTD PQSRAWEDPV EKQLQHERRR
460 470 480 490 500
RQQSAPQVAV NGHRDLEPES EPQLESETAG KWVLCNYDFQ ARNSSELSVK
510 520 530 540 550
QRDVLEVLDD SRKWWKVRDP AGQEGYVPYN ILTPYPGPRL HHSQSPARSL
560 570 580 590 600
NSTPPPPPAP APAPPPALAR PRWDRPRWDS CDSLNGLDPS EKEKFSQMLI
610 620 630 640 650
VNEELQARLA QGRSGPSRAV PGPRAPEPQL SPGSDASEVR AWLQAKGFSS
660 670 680 690 700
GTVDALGVLT GAQLFSLQKE ELRAVSPEEG ARVYSQVTVQ RSLLEDKEKV
710 720
SELEAVMEKQ KKKVEGEVEM EVI
Length:723
Mass (Da):80,251
Last modified:May 18, 2010 - v3
Checksum:iE55F71B6E128151E
GO
Isoform 2 (identifier: Q8TE68-2) [UniParc]FASTAAdd to basket
Also known as: B

The sequence of this isoform differs from the canonical sequence as follows:
     1-127: Missing.
     128-143: ERAQPDVHFFQGLRLG → MNRTWPRRIWGSSQDE

Show »
Length:596
Mass (Da):66,477
Checksum:iCA417997A8419B8F
GO
Isoform 3 (identifier: Q8TE68-3) [UniParc]FASTAAdd to basket
Also known as: C

The sequence of this isoform differs from the canonical sequence as follows:
     1-39: MSTATGPEAAPKPSAKSIYEQRKRYSTVVMADVSQYPVN → MGRKAIVLAIANTSLAFPLCQ
     451-451: R → RQVTQATQQGRGWEVRGRGRSAWPRLTRLSYFL
     509-696: DDSRKWWKVR...VTVQRSLLED → ED

Show »
Length:551
Mass (Da):61,682
Checksum:i39325B1AB8281946
GO
Isoform 4 (identifier: Q8TE68-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-177: Missing.
     178-187: QRDRSPAAET → MSPLSPGSPL
     191-300: QRRPSVRAVI...GRRSRRRAAG → ARADLTAILTGCPPLSACLVLAPRPHRRARLLPS
     451-451: R → RQVTQATQQGRGWEVRGRGRSAWPRLTRLSYFL

Show »
Length:502
Mass (Da):55,807
Checksum:i03BA1843CECD229A
GO

Sequence cautioni

The sequence AAG03038 differs from that shown. Reason: Frameshift at position 596.Curated
The sequence AAG03039 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti30M → T in BAC11399 (PubMed:14702039).Curated1
Sequence conflicti555P → S in AAL76117 (PubMed:12620401).Curated1
Sequence conflicti688T → I in BAA91041 (PubMed:14702039).Curated1
Sequence conflicti695E → G in BAC11399 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0603754A → T2 PublicationsCorresponds to variant dbSNP:rs12609976Ensembl.1
Natural variantiVAR_060376288R → G. Corresponds to variant dbSNP:rs1620074Ensembl.1
Natural variantiVAR_056870457Q → E2 PublicationsCorresponds to variant dbSNP:rs1628576Ensembl.1
Natural variantiVAR_060377669K → R3 PublicationsCorresponds to variant dbSNP:rs1054940Ensembl.1
Natural variantiVAR_061647703L → P. Corresponds to variant dbSNP:rs60073068Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0190821 – 177Missing in isoform 4. 1 PublicationAdd BLAST177
Alternative sequenceiVSP_0190831 – 127Missing in isoform 2. 2 PublicationsAdd BLAST127
Alternative sequenceiVSP_0190841 – 39MSTAT…QYPVN → MGRKAIVLAIANTSLAFPLC Q in isoform 3. 1 PublicationAdd BLAST39
Alternative sequenceiVSP_019085128 – 143ERAQP…GLRLG → MNRTWPRRIWGSSQDE in isoform 2. 2 PublicationsAdd BLAST16
Alternative sequenceiVSP_019086178 – 187QRDRSPAAET → MSPLSPGSPL in isoform 4. 1 Publication10
Alternative sequenceiVSP_019087191 – 300QRRPS…RRAAG → ARADLTAILTGCPPLSACLV LAPRPHRRARLLPS in isoform 4. 1 PublicationAdd BLAST110
Alternative sequenceiVSP_019088451R → RQVTQATQQGRGWEVRGRGR SAWPRLTRLSYFL in isoform 3 and isoform 4. 2 Publications1
Alternative sequenceiVSP_019089509 – 696DDSRK…SLLED → ED in isoform 3. 1 PublicationAdd BLAST188

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF282168 Genomic DNA Translation: AAG03039.1 Sequence problems.
AF282167 mRNA Translation: AAG03038.1 Frameshift.
AY074928 mRNA Translation: AAL76117.1
AF370395 mRNA Translation: AAQ15231.1
AK000265 mRNA Translation: BAA91041.1
AK075098 mRNA Translation: BAC11399.1
AC005782 Genomic DNA No translation available.
AC011476 Genomic DNA No translation available.
BC004907 mRNA Translation: AAH04907.2
BC015763 mRNA Translation: AAH15763.1
CCDSiCCDS12914.1 [Q8TE68-1]
CCDS12915.1 [Q8TE68-2]
RefSeqiNP_060199.3, NM_017729.3 [Q8TE68-2]
NP_573441.2, NM_133180.2 [Q8TE68-1]
UniGeneiHs.438862

Genome annotation databases

EnsembliENST00000201647; ENSP00000201647; ENSG00000131037 [Q8TE68-1]
ENST00000245618; ENSP00000245618; ENSG00000131037 [Q8TE68-2]
GeneIDi54869
KEGGihsa:54869
UCSCiuc002qis.5 human [Q8TE68-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiES8L1_HUMAN
AccessioniPrimary (citable) accession number: Q8TE68
Secondary accession number(s): Q71RE2
, Q8NC10, Q96BB7, Q9BSQ2, Q9GZQ2, Q9NXH0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: May 18, 2010
Last modified: June 20, 2018
This is version 132 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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