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Protein

Epidermal growth factor receptor kinase substrate 8-like protein 3

Gene

EPS8L3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Names & Taxonomyi

Protein namesi
Recommended name:
Epidermal growth factor receptor kinase substrate 8-like protein 3
Short name:
EPS8-like protein 3
Alternative name(s):
Epidermal growth factor receptor pathway substrate 8-related protein 3
Short name:
EPS8-related protein 3
Gene namesi
Name:EPS8L3
Synonyms:EPS8R3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 1

Organism-specific databases

HGNCiHGNC:21297. EPS8L3.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

Orphaneti444. Marie Unna hereditary hypotrichosis.
PharmGKBiPA134940846.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 593593Epidermal growth factor receptor kinase substrate 8-like protein 3PRO_0000239087Add
BLAST

Proteomic databases

MaxQBiQ8TE67.
PaxDbiQ8TE67.
PRIDEiQ8TE67.

PTM databases

PhosphoSiteiQ8TE67.

Expressioni

Gene expression databases

BgeeiQ8TE67.
CleanExiHS_EPS8L3.
GenevestigatoriQ8TE67.

Organism-specific databases

HPAiHPA030998.

Interactioni

Subunit structurei

Interacts with ABI1. Part of a complex that contains SOS1, ABI1 and EPS8L2. Interacts with FASLG.2 Publications

Protein-protein interaction databases

BioGridi122721. 5 interactions.
IntActiQ8TE67. 3 interactions.
MINTiMINT-7213997.
STRINGi9606.ENSP00000358820.

Structurei

Secondary structure

1
593
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi453 – 4597Combined sources
Beta strandi465 – 4684Combined sources
Beta strandi476 – 4816Combined sources
Beta strandi483 – 4908Combined sources
Beta strandi496 – 5005Combined sources
Helixi501 – 5033Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WXTNMR-A453-507[»]
ProteinModelPortaliQ8TE67.
SMRiQ8TE67. Positions 28-148, 452-513, 522-589.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8TE67.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini450 – 50960SH3PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi170 – 23566Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the EPS8 family.Curated
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiNOG277915.
GeneTreeiENSGT00390000003646.
HOVERGENiHBG099838.
InParanoidiQ8TE67.
KOiK17277.
OMAiWLQAENF.
OrthoDBiEOG7TMZR9.
PhylomeDBiQ8TE67.
TreeFamiTF313069.

Family and domain databases

InterProiIPR013625. PTB.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF08416. PTB. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8TE67-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSRPSSRAIY LHRKEYSQNL TSEPTLLQHR VEHLMTCKQG SQRVQGPEDA
60 70 80 90 100
LQKLFEMDAQ GRVWSQDLIL QVRDGWLQLL DIETKEELDS YRLDSIQAMN
110 120 130 140 150
VALNTCSYNS ILSITVQEPG LPGTSTLLFQ CQEVGAERLK TSLQKALEEE
160 170 180 190 200
LEQRPRLGGL QPGQDRWRGP AMERPLPMEQ ARYLEPGIPP EQPHQRTLEH
210 220 230 240 250
SLPPSPRPLP RHTSAREPSA FTLPPPRRSS SPEDPERDEE VLNHVLRDIE
260 270 280 290 300
LFMGKLEKAQ AKTSRKKKFG KKNKDQGGLT QAQYIDCFQK IKHSFNLLGR
310 320 330 340 350
LATWLKETSA PELVHILFKS LNFILARCPE AGLAAQVISP LLTPKAINLL
360 370 380 390 400
QSCLSPPESN LWMGLGPAWT TSRADWTGDE PLPYQPTFSD DWQLPEPSSQ
410 420 430 440 450
APLGYQDPVS LRRGSHRLGS TSHFPQEKTH NHDPQPGDPN SRPSSPKPAQ
460 470 480 490 500
PALKMQVLYE FEARNPRELT VVQGEKLEVL DHSKRWWLVK NEAGRSGYIP
510 520 530 540 550
SNILEPLQPG TPGTQGQSPS RVPMLRLSSR PEEVTDWLQA ENFSTATVRT
560 570 580 590
LGSLTGSQLL RIRPGELQML CPQEAPRILS RLEAVRRMLG ISP
Length:593
Mass (Da):66,861
Last modified:May 30, 2006 - v2
Checksum:i2BB9B52ADD338F88
GO
Isoform 2 (identifier: Q8TE67-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     412-441: Missing.

Show »
Length:563
Mass (Da):63,502
Checksum:iA3D9199068057B9F
GO
Isoform 3 (identifier: Q8TE67-3) [UniParc]FASTAAdd to Basket

Also known as: A

The sequence of this isoform differs from the canonical sequence as follows:
     153-153: Q → QS

Show »
Length:594
Mass (Da):66,948
Checksum:iE114E2211130CAAA
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti35 – 351M → I.
Corresponds to variant rs17598321 [ dbSNP | Ensembl ].
VAR_050976
Natural varianti163 – 1631G → S.1 Publication
Corresponds to variant rs6693815 [ dbSNP | Ensembl ].
VAR_026580
Natural varianti293 – 2931H → Y.2 Publications
Corresponds to variant rs3818562 [ dbSNP | Ensembl ].
VAR_026581
Natural varianti356 – 3561P → S.
Corresponds to variant rs11102001 [ dbSNP | Ensembl ].
VAR_026582
Natural varianti581 – 5811R → Q.
Corresponds to variant rs35072794 [ dbSNP | Ensembl ].
VAR_050977

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei153 – 1531Q → QS in isoform 3. 1 PublicationVSP_019095
Alternative sequencei412 – 44130Missing in isoform 2. 1 PublicationVSP_019096Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY074930 mRNA. Translation: AAL76119.1.
AK025175 mRNA. Translation: BAB15081.1.
AK292198 mRNA. Translation: BAF84887.1.
AL158847 Genomic DNA. Translation: CAI22582.1.
AL158847 Genomic DNA. Translation: CAI22583.1.
AL158847 Genomic DNA. Translation: CAI22584.1.
BC012926 mRNA. Translation: AAH12926.1.
CCDSiCCDS813.1. [Q8TE67-2]
CCDS814.1. [Q8TE67-1]
CCDS815.1. [Q8TE67-3]
RefSeqiNP_078802.2. NM_024526.3. [Q8TE67-2]
NP_573444.2. NM_133181.3. [Q8TE67-1]
NP_620641.1. NM_139053.2. [Q8TE67-3]
UniGeneiHs.485352.

Genome annotation databases

EnsembliENST00000361852; ENSP00000354551; ENSG00000198758. [Q8TE67-2]
ENST00000361965; ENSP00000355255; ENSG00000198758. [Q8TE67-1]
ENST00000369805; ENSP00000358820; ENSG00000198758. [Q8TE67-3]
GeneIDi79574.
KEGGihsa:79574.
UCSCiuc001dyq.2. human. [Q8TE67-3]
uc001dyr.2. human. [Q8TE67-1]
uc001dys.2. human. [Q8TE67-2]

Polymorphism databases

DMDMi108864727.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY074930 mRNA. Translation: AAL76119.1.
AK025175 mRNA. Translation: BAB15081.1.
AK292198 mRNA. Translation: BAF84887.1.
AL158847 Genomic DNA. Translation: CAI22582.1.
AL158847 Genomic DNA. Translation: CAI22583.1.
AL158847 Genomic DNA. Translation: CAI22584.1.
BC012926 mRNA. Translation: AAH12926.1.
CCDSiCCDS813.1. [Q8TE67-2]
CCDS814.1. [Q8TE67-1]
CCDS815.1. [Q8TE67-3]
RefSeqiNP_078802.2. NM_024526.3. [Q8TE67-2]
NP_573444.2. NM_133181.3. [Q8TE67-1]
NP_620641.1. NM_139053.2. [Q8TE67-3]
UniGeneiHs.485352.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WXTNMR-A453-507[»]
ProteinModelPortaliQ8TE67.
SMRiQ8TE67. Positions 28-148, 452-513, 522-589.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122721. 5 interactions.
IntActiQ8TE67. 3 interactions.
MINTiMINT-7213997.
STRINGi9606.ENSP00000358820.

PTM databases

PhosphoSiteiQ8TE67.

Polymorphism databases

DMDMi108864727.

Proteomic databases

MaxQBiQ8TE67.
PaxDbiQ8TE67.
PRIDEiQ8TE67.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000361852; ENSP00000354551; ENSG00000198758. [Q8TE67-2]
ENST00000361965; ENSP00000355255; ENSG00000198758. [Q8TE67-1]
ENST00000369805; ENSP00000358820; ENSG00000198758. [Q8TE67-3]
GeneIDi79574.
KEGGihsa:79574.
UCSCiuc001dyq.2. human. [Q8TE67-3]
uc001dyr.2. human. [Q8TE67-1]
uc001dys.2. human. [Q8TE67-2]

Organism-specific databases

CTDi79574.
GeneCardsiGC01M110292.
HGNCiHGNC:21297. EPS8L3.
HPAiHPA030998.
MIMi614989. gene.
neXtProtiNX_Q8TE67.
Orphaneti444. Marie Unna hereditary hypotrichosis.
PharmGKBiPA134940846.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG277915.
GeneTreeiENSGT00390000003646.
HOVERGENiHBG099838.
InParanoidiQ8TE67.
KOiK17277.
OMAiWLQAENF.
OrthoDBiEOG7TMZR9.
PhylomeDBiQ8TE67.
TreeFamiTF313069.

Miscellaneous databases

EvolutionaryTraceiQ8TE67.
GeneWikiiEPS8L3.
GenomeRNAii79574.
NextBioi68546.
PROiQ8TE67.
SOURCEiSearch...

Gene expression databases

BgeeiQ8TE67.
CleanExiHS_EPS8L3.
GenevestigatoriQ8TE67.

Family and domain databases

InterProiIPR013625. PTB.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF08416. PTB. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "In silico analysis of the EPS8 gene family: genomic organization, expression profile, and protein structure."
    Tocchetti A., Confalonieri S., Scita G., Di Fiore P.P., Betsholtz C.
    Genomics 81:234-244(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT SER-163.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT TYR-293.
    Tissue: Colon.
  3. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), VARIANT TYR-293.
    Tissue: Colon.
  5. "The eps8 family of proteins links growth factor stimulation to actin reorganization generating functional redundancy in the Ras/Rac pathway."
    Offenhaeuser N., Borgonovo A., Disanza A., Romano P., Ponzanelli I., Iannolo G., Di Fiore P.P., Scita G.
    Mol. Biol. Cell 15:91-98(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN A COMPLEX WITH ABI1 AND SOS1, INTERACTION WITH ABI1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  6. "Identification of SH3 domain interaction partners of human FasL (CD178) by phage display screening."
    Voss M., Lettau M., Janssen O.
    BMC Immunol. 10:53-53(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH FASLG.
  7. "Solution structure of the SH3 domain of human hypothetical protein FLJ21522."
    RIKEN structural genomics initiative (RSGI)
    Submitted (JAN-2006) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 453-507.

Entry informationi

Entry nameiES8L3_HUMAN
AccessioniPrimary (citable) accession number: Q8TE67
Secondary accession number(s): A8K833
, Q5T8Q6, Q5T8Q7, Q5T8Q8, Q96E47, Q9H719
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: May 30, 2006
Last modified: January 7, 2015
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.