Q8TE67 (ES8L3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Epidermal growth factor receptor kinase substrate 8-like protein 3
Short name=EPS8-like protein 3

Alternative name(s):
Epidermal growth factor receptor pathway substrate 8-related protein 3
Short name=EPS8-related protein 3

Gene names

Name:	EPS8L3
Synonyms:	EPS8R3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	593 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Interacts with ABI1. Part of a complex that contains SOS1, ABI1 and EPS8L2. Interacts with FASLG. Ref.5 Ref.6
Subcellular location	Cytoplasm Ref.5.
Sequence similarities	Belongs to the EPS8 family. Contains 1 SH3 domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Domain	SH3 domain
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8TE67-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8TE67-2)

The sequence of this isoform differs from the canonical sequence as follows:
     412-441: Missing.

Isoform 3 (identifier: Q8TE67-3)

Also known as: A;

The sequence of this isoform differs from the canonical sequence as follows:
     153-153: Q → QS

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 593

593

Epidermal growth factor receptor kinase substrate 8-like protein 3

PRO_0000239087

Regions

Domain

450 – 509

60

SH3

Compositional bias

170 – 235

66

Pro-rich

Natural variations

Alternative sequence

153

1

Q → QS in isoform 3.

VSP_019095

Alternative sequence

412 – 441

30

Missing in isoform 2.

VSP_019096

Natural variant

35

1

M → I.
Corresponds to variant rs17598321 [ dbSNP | Ensembl ].

VAR_050976

Natural variant

163

1

G → S. Ref.1
Corresponds to variant rs6693815 [ dbSNP | Ensembl ].

VAR_026580

Natural variant

293

1

H → Y. Ref.2 Ref.4
Corresponds to variant rs3818562 [ dbSNP | Ensembl ].

VAR_026581

Natural variant

356

1

P → S.
Corresponds to variant rs11102001 [ dbSNP | Ensembl ].

VAR_026582

Natural variant

581

1

R → Q.
Corresponds to variant rs35072794 [ dbSNP | Ensembl ].

VAR_050977

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

593

Helix Strand Turn

Details...

Beta strand

453 – 459

7

Beta strand

465 – 468

4

Beta strand

476 – 481

6

Beta strand

483 – 490

8

Beta strand

496 – 500

5

Helix

501 – 503

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 30, 2006. Version 2. Checksum: 2BB9B52ADD338F88	FASTA	593	66,861
	10 20 30 40 50 60 MSRPSSRAIY LHRKEYSQNL TSEPTLLQHR VEHLMTCKQG SQRVQGPEDA LQKLFEMDAQ 70 80 90 100 110 120 GRVWSQDLIL QVRDGWLQLL DIETKEELDS YRLDSIQAMN VALNTCSYNS ILSITVQEPG 130 140 150 160 170 180 LPGTSTLLFQ CQEVGAERLK TSLQKALEEE LEQRPRLGGL QPGQDRWRGP AMERPLPMEQ 190 200 210 220 230 240 ARYLEPGIPP EQPHQRTLEH SLPPSPRPLP RHTSAREPSA FTLPPPRRSS SPEDPERDEE 250 260 270 280 290 300 VLNHVLRDIE LFMGKLEKAQ AKTSRKKKFG KKNKDQGGLT QAQYIDCFQK IKHSFNLLGR 310 320 330 340 350 360 LATWLKETSA PELVHILFKS LNFILARCPE AGLAAQVISP LLTPKAINLL QSCLSPPESN 370 380 390 400 410 420 LWMGLGPAWT TSRADWTGDE PLPYQPTFSD DWQLPEPSSQ APLGYQDPVS LRRGSHRLGS 430 440 450 460 470 480 TSHFPQEKTH NHDPQPGDPN SRPSSPKPAQ PALKMQVLYE FEARNPRELT VVQGEKLEVL 490 500 510 520 530 540 DHSKRWWLVK NEAGRSGYIP SNILEPLQPG TPGTQGQSPS RVPMLRLSSR PEEVTDWLQA 550 560 570 580 590 ENFSTATVRT LGSLTGSQLL RIRPGELQML CPQEAPRILS RLEAVRRMLG ISP
	Isoform 2 [UniParc]. Checksum: A3D9199068057B9F	FASTA	563	63,502
	10 20 30 40 50 60 MSRPSSRAIY LHRKEYSQNL TSEPTLLQHR VEHLMTCKQG SQRVQGPEDA LQKLFEMDAQ 70 80 90 100 110 120 GRVWSQDLIL QVRDGWLQLL DIETKEELDS YRLDSIQAMN VALNTCSYNS ILSITVQEPG 130 140 150 160 170 180 LPGTSTLLFQ CQEVGAERLK TSLQKALEEE LEQRPRLGGL QPGQDRWRGP AMERPLPMEQ 190 200 210 220 230 240 ARYLEPGIPP EQPHQRTLEH SLPPSPRPLP RHTSAREPSA FTLPPPRRSS SPEDPERDEE 250 260 270 280 290 300 VLNHVLRDIE LFMGKLEKAQ AKTSRKKKFG KKNKDQGGLT QAQYIDCFQK IKHSFNLLGR 310 320 330 340 350 360 LATWLKETSA PELVHILFKS LNFILARCPE AGLAAQVISP LLTPKAINLL QSCLSPPESN 370 380 390 400 410 420 LWMGLGPAWT TSRADWTGDE PLPYQPTFSD DWQLPEPSSQ APLGYQDPVS LRPSSPKPAQ 430 440 450 460 470 480 PALKMQVLYE FEARNPRELT VVQGEKLEVL DHSKRWWLVK NEAGRSGYIP SNILEPLQPG 490 500 510 520 530 540 TPGTQGQSPS RVPMLRLSSR PEEVTDWLQA ENFSTATVRT LGSLTGSQLL RIRPGELQML 550 560 CPQEAPRILS RLEAVRRMLG ISP
	Isoform 3 (A) [UniParc]. Checksum: E114E2211130CAAA	FASTA	594	66,948
	10 20 30 40 50 60 MSRPSSRAIY LHRKEYSQNL TSEPTLLQHR VEHLMTCKQG SQRVQGPEDA LQKLFEMDAQ 70 80 90 100 110 120 GRVWSQDLIL QVRDGWLQLL DIETKEELDS YRLDSIQAMN VALNTCSYNS ILSITVQEPG 130 140 150 160 170 180 LPGTSTLLFQ CQEVGAERLK TSLQKALEEE LEQSRPRLGG LQPGQDRWRG PAMERPLPME 190 200 210 220 230 240 QARYLEPGIP PEQPHQRTLE HSLPPSPRPL PRHTSAREPS AFTLPPPRRS SSPEDPERDE 250 260 270 280 290 300 EVLNHVLRDI ELFMGKLEKA QAKTSRKKKF GKKNKDQGGL TQAQYIDCFQ KIKHSFNLLG 310 320 330 340 350 360 RLATWLKETS APELVHILFK SLNFILARCP EAGLAAQVIS PLLTPKAINL LQSCLSPPES 370 380 390 400 410 420 NLWMGLGPAW TTSRADWTGD EPLPYQPTFS DDWQLPEPSS QAPLGYQDPV SLRRGSHRLG 430 440 450 460 470 480 STSHFPQEKT HNHDPQPGDP NSRPSSPKPA QPALKMQVLY EFEARNPREL TVVQGEKLEV 490 500 510 520 530 540 LDHSKRWWLV KNEAGRSGYI PSNILEPLQP GTPGTQGQSP SRVPMLRLSS RPEEVTDWLQ 550 560 570 580 590 AENFSTATVR TLGSLTGSQL LRIRPGELQM LCPQEAPRIL SRLEAVRRML GISP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"In silico analysis of the EPS8 gene family: genomic organization, expression profile, and protein structure." Tocchetti A., Confalonieri S., Scita G., Di Fiore P.P., Betsholtz C. Genomics 81:234-244(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT SER-163.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT TYR-293. Tissue: Colon.
[3]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), VARIANT TYR-293. Tissue: Colon.
[5]	"The eps8 family of proteins links growth factor stimulation to actin reorganization generating functional redundancy in the Ras/Rac pathway." Offenhaeuser N., Borgonovo A., Disanza A., Romano P., Ponzanelli I., Iannolo G., Di Fiore P.P., Scita G. Mol. Biol. Cell 15:91-98(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN A COMPLEX WITH ABI1 AND SOS1, INTERACTION WITH ABI1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[6]	"Identification of SH3 domain interaction partners of human FasL (CD178) by phage display screening." Voss M., Lettau M., Janssen O. BMC Immunol. 10:53-53(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH FASLG.
[7]	"Solution structure of the SH3 domain of human hypothetical protein FLJ21522." RIKEN structural genomics initiative (RSGI) Submitted (JAN-2006) to the PDB data bank Cited for: STRUCTURE BY NMR OF 453-507.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY074930 mRNA. Translation: AAL76119.1.
AK025175 mRNA. Translation: BAB15081.1.
AK292198 mRNA. Translation: BAF84887.1.
AL158847 Genomic DNA. Translation: CAI22582.1.
AL158847 Genomic DNA. Translation: CAI22583.1.
AL158847 Genomic DNA. Translation: CAI22584.1.
BC012926 mRNA. Translation: AAH12926.1.

IPI

IPI00181833.
IPI00256752.
IPI00302864.

RefSeq

NP_078802.2. NM_024526.3.
NP_573444.2. NM_133181.3.
NP_620641.1. NM_139053.2.

UniGene

Hs.485352.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1WXT	NMR	-	A	453-507	[»]

ProteinModelPortal

Q8TE67.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q8TE67. 1 interaction.

MINT

MINT-7213997.

STRING

9606.ENSP00000358820.

PTM databases

PhosphoSite

Q8TE67.

Polymorphism databases

DMDM

108864727.

Proteomic databases

PaxDb

Q8TE67.

PRIDE

Q8TE67.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000361852; ENSP00000354551; ENSG00000198758.
ENST00000361965; ENSP00000355255; ENSG00000198758.
ENST00000369805; ENSP00000358820; ENSG00000198758.

GeneID

79574.

KEGG

hsa:79574.

UCSC

uc001dyq.2. human.
uc001dyr.2. human.
uc001dys.2. human.

Organism-specific databases

CTD

79574.

GeneCards

GC01M110292.

HGNC

HGNC:21297. EPS8L3.

HPA

HPA030998.

MIM

614989. gene.

neXtProt

NX_Q8TE67.

PharmGKB

PA134940846.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG277915.

HOVERGEN

HBG099838.

OMA

WLQAENF.

Gene expression databases

Bgee

Q8TE67.

CleanEx

HS_EPS8L3.

Genevestigator

Q8TE67.

GermOnline

ENSG00000198758. Homo sapiens.

Family and domain databases

InterPro

IPR013625. PTB.
IPR001452. SH3_domain.
[Graphical view]

Pfam

PF08416. PTB. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]

SMART

SM00326. SH3. 1 hit.
[Graphical view]

SUPFAM

SSF50044. SH3. 1 hit.

PROSITE

PS50002. SH3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q8TE67.

GenomeRNAi

79574.

NextBio

68546.

SOURCE

Search...

Entry information

Entry name

ES8L3_HUMAN

Accession

Primary (citable) accession number: Q8TE67
Secondary accession number(s): A8K833 , Q5T8Q6, Q5T8Q7, Q5T8Q8, Q96E47, Q9H719

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	May 30, 2006
Last modified:	May 1, 2013
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.