Reviewed, UniProtKB/Swiss-Prot Q8TE58 (ATS15_HUMAN)

Names and origin

Protein names

Recommended name:
    A disintegrin and metalloproteinase with thrombospondin motifs 15
      Short name=ADAMTS-15
      Short name=ADAM-TS 15
      Short name=ADAM-TS15
    EC=3.4.24.-

Gene names

Name:

ADAMTS15

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	950 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Secreted › extracellular space › extracellular matrix By similarity.
Tissue specificity	Expressed in fetal liver and kidney, but not in any of the adult tissues examined.
Domain	The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix By similarity. The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational modification	The precursor is cleaved by a furin endopeptidase By similarity.
Sequence similarities	Contains 1 disintegrin domain. Contains 1 peptidase M12B domain. Contains 3 TSP type-1 domains.

Ontologies

Keywords
Cellular component	Extracellular matrix Secreted
Coding sequence diversity	Polymorphism
Domain	Repeat Signal
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Propeptide	18 – 212	195	By similarity		PRO_0000029192
	Chain	213 – 950	738	A disintegrin and metalloproteinase with thrombospondin motifs 15		PRO_0000029193
Regions
	Domain	218 – 427	210	Peptidase M12B
	Domain	428 – 515	88	Disintegrin
	Domain	516 – 571	56	TSP type-1 1
	Domain	839 – 895	57	TSP type-1 2
	Domain	896 – 949	54	TSP type-1 3
	Region	701 – 838	138	Spacer
	Motif	172 – 179	8	Cysteine switch By similarity
	Compositional bias	572 – 700	129	Cys-rich
Sites
	Active site	362	1	By similarity
	Metal binding	174	1	Zinc; in inhibited form By similarity
	Metal binding	361	1	Zinc; catalytic By similarity
	Metal binding	365	1	Zinc; catalytic By similarity
	Metal binding	371	1	Zinc; catalytic By similarity
Amino acid modifications
	Glycosylation	141	1	N-linked (GlcNAc...) Potential
	Glycosylation	591	1	N-linked (GlcNAc...) Potential
	Glycosylation	623	1	N-linked (GlcNAc...) Potential
	Glycosylation	679	1	N-linked (GlcNAc...) Potential
	Disulfide bond	339 ↔ 422		By similarity
	Disulfide bond	377 ↔ 406		By similarity
	Disulfide bond	528 ↔ 565		By similarity
	Disulfide bond	532 ↔ 570		By similarity
	Disulfide bond	543 ↔ 555		By similarity
Natural variations
	Natural variant	623	1	N → S: dbSNP rs11222114.		VAR_051594
	Natural variant	770	1	Q → R in a colorectal cancer sample; somatic mutation. Ref.3		VAR_036150
	Natural variant	878	1	C → G in a colorectal cancer sample; somatic mutation. Ref.3		VAR_036151

Sequences

	Sequence		Length	Mass (Da)
	Q8TE58-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 5DFBE18285CCCC3B	FASTA	950	103,287
	10 20 30 40 50 60 MLLLGILTLA FAGRTAGGSE PEREVVVPIR LDPDINGRRY YWRGPEDSGD QGLIFQITAF 70 80 90 100 110 120 QEDFYLHLTP DAQFLAPAFS TEHLGVPLQG LTGGSSDLRR CFYSGDVNAE PDSFAAVSLC 130 140 150 160 170 180 GGLRGAFGYR GAEYVISPLP NASAPAAQRN SQGAHLLQRR GVPGGPSGDP TSRCGVASGW 190 200 210 220 230 240 NPAILRALDP YKPRRAGFGE SRSRRRSGRA KRFVSIPRYV ETLVVADESM VKFHGADLEH 250 260 270 280 290 300 YLLTLLATAA RLYRHPSILN PINIVVVKVL LLRDRDSGPK VTGNAALTLR NFCAWQKKLN 310 320 330 340 350 360 KVSDKHPEYW DTAILFTRQD LCGATTCDTL GMADVGTMCD PKRSCSVIED DGLPSAFTTA 370 380 390 400 410 420 HELGHVFNMP HDNVKVCEEV FGKLRANHMM SPTLIQIDRA NPWSACSAAI ITDFLDSGHG 430 440 450 460 470 480 DCLLDQPSKP ISLPEDLPGA SYTLSQQCEL AFGVGSKPCP YMQYCTKLWC TGKAKGQMVC 490 500 510 520 530 540 QTRHFPWADG TSCGEGKLCL KGACVERHNL NKHRVDGSWA KWDPYGPCSR TCGGGVQLAR 550 560 570 580 590 600 RQCTNPTPAN GGKYCEGVRV KYRSCNLEPC PSSASGKSFR EEQCEAFNGY NHSTNRLTLA 610 620 630 640 650 660 VAWVPKYSGV SPRDKCKLIC RANGTGYFYV LAPKVVDGTL CSPDSTSVCV QGKCIKAGCD 670 680 690 700 710 720 GNLGSKKRFD KCGVCGGDNK SCKKVTGLFT KPMHGYNFVV AIPAGASSID IRQRGYKGLI 730 740 750 760 770 780 GDDNYLALKN SQGKYLLNGH FVVSAVERDL VVKGSLLRYS GTGTAVESLQ ASRPILEPLT 790 800 810 820 830 840 VEVLSVGKMT PPRVRYSFYL PKEPREDKSS HPKDPRGPSV LHNSVLSLSN QVEQPDDRPP 850 860 870 880 890 900 ARWVAGSWGP CSASCGSGLQ KRAVDCRGSA GQRTVPACDA AHRPVETQAC GEPCPTWELS 910 920 930 940 950 AWSPCSKSCG RGFQRRSLKC VGHGGRLLAR DQCNLHRKPQ ELDFCVLRPC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, expression analysis, and structural characterization of seven novel human ADAMTSs, a family of metalloproteinases with disintegrin and thrombospondin-1 domains." Cal S., Obaya A.J., Llamazares M., Garabaya C., Quesada V., Lopez-Otin C. Gene 283:49-62(2002) [PubMed: 11867212] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] ARG-770 AND GLY-878.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ315733 mRNA. Translation: CAC86014.1. BC109114 mRNA. Translation: AAI09115.1.
IPI	IPI00152637.
RefSeq	NP_620686.1.
UniGene	Hs.681791
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q8TE58.
Proteomic databases
PRIDE	Q8TE58.
Genome annotation databases
Ensembl	ENST00000299164; ENSP00000299164; ENSG00000166106; Homo sapiens. [Genome view]
GeneID	170689.
KEGG	hsa:170689.
UCSC	uc001qgh.1. human.
Organism-specific databases
CTD	170689.
GeneCards	GC11P129824.
H-InvDB	HIX0059057.
HGNC	HGNC:16305. ADAMTS15.
MIM	607509. gene.
PharmGKB	PA24541.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q8TE58.
HOVERGEN	Q8TE58.
OMA	RRCFYSG
OrthoDB	EOG96HJWF
Gene expression databases
ArrayExpress	Q8TE58.
Bgee	Q8TE58.
CleanEx	HS_ADAMTS15.
Genevestigator	Q8TE58.
GermOnline	ENSG00000166106. Homo sapiens.
Family and domain databases
InterPro	IPR010294. ADAM_spacer1. IPR013277. Pept_M12B_ADAM-TS8. IPR001590. Peptidase_M12B. IPR013273. Peptidase_M12B_ADAM-TS. IPR002870. Peptidase_M12B_N. IPR000884. Thrombospondin_1_rpt. [Graphical view]
Pfam	PF05986. ADAM_spacer1. 1 hit. PF01562. Pep_M12B_propep. 1 hit. PF01421. Reprolysin. 1 hit. PF00090. TSP_1. 3 hits. [Graphical view]
PRINTS	PR01861. ADAMTS8. PR01857. ADAMTSFAMILY.
SMART	SM00209. TSP1. 3 hits. [Graphical view]
PROSITE	PS50215. ADAM_MEPRO. 1 hit. PS00546. CYSTEINE_SWITCH. False negative. PS00427. DISINTEGRIN_1. False negative. PS50214. DISINTEGRIN_2. False negative. PS50092. TSP1. 3 hits. PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	89098.
SOURCE	Search...

Entry information

Entry name

ATS15_HUMAN

Accession

Primary (citable) accession number: Q8TE58
Secondary accession number(s): Q32MI6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 28, 2003
Last sequence update:	June 1, 2002
Last modified:	November 24, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.