Q8TE57 (ATS16_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
A disintegrin and metalloproteinase with thrombospondin motifs 16
Short name=ADAM-TS 16
Short name=ADAM-TS16
Short name=ADAMTS-16
EC=3.4.24.-

Gene names

Name:	ADAMTS16
Synonyms:	KIAA2029

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1224 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Secreted › extracellular space › extracellular matrix By similarity.
Tissue specificity	Expressed in fetal lung and kidney and in adult prostate and ovary.
Domain	The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix By similarity. The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational modification	The precursor is cleaved by a furin endopeptidase By similarity. Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion By similarity.
Sequence similarities	Contains 1 disintegrin domain. Contains 1 peptidase M12B domain. Contains 1 PLAC domain. Contains 6 TSP type-1 domains.

Ontologies

Keywords
Cellular component	Extracellular matrix Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat Signal
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8TE57-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8TE57-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1063-1072: CSVTCERGTQ → VGALVSRERG
     1073-1224: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Propeptide	25 – 279	255	By similarity		PRO_0000029194
	Chain	280 – 1224	945	A disintegrin and metalloproteinase with thrombospondin motifs 16		PRO_0000029195
Regions
	Domain	290 – 495	206	Peptidase M12B
	Domain	496 – 585	90	Disintegrin
	Domain	586 – 641	56	TSP type-1 1
	Domain	874 – 922	49	TSP type-1 2
	Domain	927 – 987	61	TSP type-1 3
	Domain	988 – 1048	61	TSP type-1 4
	Domain	1051 – 1115	65	TSP type-1 5
	Domain	1127 – 1181	55	TSP type-1 6
	Domain	1186 – 1223	38	PLAC
	Region	747 – 873	127	Spacer
	Motif	247 – 254	8	Cysteine switch By similarity
	Compositional bias	642 – 746	105	Cys-rich
Sites
	Active site	434	1	By similarity
	Metal binding	249	1	Zinc; in inhibited form By similarity
	Metal binding	433	1	Zinc; catalytic By similarity
	Metal binding	437	1	Zinc; catalytic By similarity
	Metal binding	443	1	Zinc; catalytic By similarity
Amino acid modifications
	Glycosylation	156	1	N-linked (GlcNAc...) Potential
	Glycosylation	310	1	N-linked (GlcNAc...) Potential
	Glycosylation	741	1	N-linked (GlcNAc...) Potential
	Glycosylation	780	1	N-linked (GlcNAc...) Potential
	Glycosylation	835	1	N-linked (GlcNAc...) Potential
	Glycosylation	905	1	N-linked (GlcNAc...) Potential
	Glycosylation	935	1	N-linked (GlcNAc...) Potential
	Disulfide bond	411 ↔ 490		By similarity
	Disulfide bond	450 ↔ 474		By similarity
	Disulfide bond	598 ↔ 635		By similarity
	Disulfide bond	602 ↔ 640		By similarity
	Disulfide bond	613 ↔ 625		By similarity
	Disulfide bond	939 ↔ 981		By similarity
	Disulfide bond	943 ↔ 986		By similarity
	Disulfide bond	954 ↔ 970		By similarity
Natural variations
	Alternative sequence	1063 – 1072	10	CSVTCERGTQ → VGALVSRERG in isoform 2.		VSP_007664
	Alternative sequence	1073 – 1224	152	Missing in isoform 2.		VSP_007665
	Natural variant	110	1	M → V. Corresponds to variant rs1863968 [ dbSNP | Ensembl ].		VAR_057076
	Natural variant	486	1	A → T. Corresponds to variant rs16875054 [ dbSNP | Ensembl ].		VAR_057077
	Natural variant	789	1	R → C. Corresponds to variant rs9313105 [ dbSNP | Ensembl ].		VAR_057078
	Natural variant	859	1	R → L. Corresponds to variant rs16875122 [ dbSNP | Ensembl ].		VAR_057079
	Natural variant	863	1	E → K. Corresponds to variant rs35394775 [ dbSNP | Ensembl ].		VAR_057080
Experimental info
	Sequence conflict	90	1	P → A in CAC86015. Ref.1
	Sequence conflict	104	1	S → P in CAC86015. Ref.1
	Sequence conflict	284	1	S → Y in BAC23125. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 18, 2010. Version 3. Checksum: FB6115E6C97A9683	FASTA	1,224	136,203
	10 20 30 40 50 60 MKPRARGWRG LAALWMLLAQ VAEQAPACAM GPAAAAPGSP SVPRPPPPAE RPGWMEKGEY 70 80 90 100 110 120 DLVSAYEVDH RGDYVSHEIM HHQRRRRAVP VSEVESLHLR LKGSRHDFHM DLRTSSSLVA 130 140 150 160 170 180 PGFIVQTLGK TGTKSVQTLP PEDFCFYQGS LRSHRNSSVA LSTCQGLSGM IRTEEADYFL 190 200 210 220 230 240 RPLPSHLSWK LGRAAQGSSP SHVLYKRSTE PHAPGASEVL VTSRTWELAH QPLHSSDLRL 250 260 270 280 290 300 GLPQKQHFCG RRKKYMPQPP KEDLFILPDE YKSCLRHKRS LLRSHRNEEL NVETLVVVDK 310 320 330 340 350 360 KMMQNHGHEN ITTYVLTILN MVSALFKDGT IGGNINIAIV GLILLEDEQP GLVISHHADH 370 380 390 400 410 420 TLSSFCQWQS GLMGKDGTRH DHAILLTGLD ICSWKNEPCD TLGFAPISGM CSKYRSCTIN 430 440 450 460 470 480 EDTGLGLAFT IAHESGHNFG MIHDGEGNMC KKSEGNIMSP TLAGRNGVFS WSPCSRQYLH 490 500 510 520 530 540 KFLSTAQAIC LADQPKPVKE YKYPEKLPGE LYDANTQCKW QFGEKAKLCM LDFKKDICKA 550 560 570 580 590 600 LWCHRIGRKC ETKFMPAAEG TICGHDMWCR GGQCVKYGDE GPKPTHGHWS DWSSWSPCSR 610 620 630 640 650 660 TCGGGVSHRS RLCTNPKPSH GGKFCEGSTR TLKLCNSQKC PRDSVDFRAA QCAEHNSRRF 670 680 690 700 710 720 RGRHYKWKPY TQVEDQDLCK LYCIAEGFDF FFSLSNKVKD GTPCSEDSRN VCIDGICERV 730 740 750 760 770 780 GCDNVLGSDA VEDVCGVCNG NNSACTIHRG LYTKHHHTNQ YYHMVTIPSG ARSIRIYEMN 790 800 810 820 830 840 VSTSYISVRN ALRRYYLNGH WTVDWPGRYK FSGTTFDYRR SYNEPENLIA TGPTNETLIV 850 860 870 880 890 900 ELLFQGRNPG VAWEYSMPRL GTEKQPPAQP SYTWAIVRSE CSVSCGGGQM TVREGCYRDL 910 920 930 940 950 960 KFQVNMSFCN PKTRPVTGLV PCKVSACPPS WSVGNWSACS RTCGGGAQSR PVQCTRRVHY 970 980 990 1000 1010 1020 DSEPVPASLC PQPAPSSRQA CNSQSCPPAW SAGPWAECSH TCGKGWRKRA VACKSTNPSA 1030 1040 1050 1060 1070 1080 RAQLLPDAVC TSEPKPRMHE ACLLQRCHKP KKLQWLVSAW SQCSVTCERG TQKRFLKCAE 1090 1100 1110 1120 1130 1140 KYVSGKYREL ASKKCSHLPK PSLELERACA PLPCPRHPPF AAAGPSRGSW FASPWSQCTA 1150 1160 1170 1180 1190 1200 SCGGGVQTRS VQCLAGGRPA SGCLLHQKPS ASLACNTHFC PIAEKKDAFC KDYFHWCYLV 1210 1220 PQHGMCSHKF YGKQCCKTCS KSNL
	Isoform 2 [UniParc]. Checksum: 3E2C0FF34194E631	FASTA	1,072	119,507
	10 20 30 40 50 60 MKPRARGWRG LAALWMLLAQ VAEQAPACAM GPAAAAPGSP SVPRPPPPAE RPGWMEKGEY 70 80 90 100 110 120 DLVSAYEVDH RGDYVSHEIM HHQRRRRAVP VSEVESLHLR LKGSRHDFHM DLRTSSSLVA 130 140 150 160 170 180 PGFIVQTLGK TGTKSVQTLP PEDFCFYQGS LRSHRNSSVA LSTCQGLSGM IRTEEADYFL 190 200 210 220 230 240 RPLPSHLSWK LGRAAQGSSP SHVLYKRSTE PHAPGASEVL VTSRTWELAH QPLHSSDLRL 250 260 270 280 290 300 GLPQKQHFCG RRKKYMPQPP KEDLFILPDE YKSCLRHKRS LLRSHRNEEL NVETLVVVDK 310 320 330 340 350 360 KMMQNHGHEN ITTYVLTILN MVSALFKDGT IGGNINIAIV GLILLEDEQP GLVISHHADH 370 380 390 400 410 420 TLSSFCQWQS GLMGKDGTRH DHAILLTGLD ICSWKNEPCD TLGFAPISGM CSKYRSCTIN 430 440 450 460 470 480 EDTGLGLAFT IAHESGHNFG MIHDGEGNMC KKSEGNIMSP TLAGRNGVFS WSPCSRQYLH 490 500 510 520 530 540 KFLSTAQAIC LADQPKPVKE YKYPEKLPGE LYDANTQCKW QFGEKAKLCM LDFKKDICKA 550 560 570 580 590 600 LWCHRIGRKC ETKFMPAAEG TICGHDMWCR GGQCVKYGDE GPKPTHGHWS DWSSWSPCSR 610 620 630 640 650 660 TCGGGVSHRS RLCTNPKPSH GGKFCEGSTR TLKLCNSQKC PRDSVDFRAA QCAEHNSRRF 670 680 690 700 710 720 RGRHYKWKPY TQVEDQDLCK LYCIAEGFDF FFSLSNKVKD GTPCSEDSRN VCIDGICERV 730 740 750 760 770 780 GCDNVLGSDA VEDVCGVCNG NNSACTIHRG LYTKHHHTNQ YYHMVTIPSG ARSIRIYEMN 790 800 810 820 830 840 VSTSYISVRN ALRRYYLNGH WTVDWPGRYK FSGTTFDYRR SYNEPENLIA TGPTNETLIV 850 860 870 880 890 900 ELLFQGRNPG VAWEYSMPRL GTEKQPPAQP SYTWAIVRSE CSVSCGGGQM TVREGCYRDL 910 920 930 940 950 960 KFQVNMSFCN PKTRPVTGLV PCKVSACPPS WSVGNWSACS RTCGGGAQSR PVQCTRRVHY 970 980 990 1000 1010 1020 DSEPVPASLC PQPAPSSRQA CNSQSCPPAW SAGPWAECSH TCGKGWRKRA VACKSTNPSA 1030 1040 1050 1060 1070 RAQLLPDAVC TSEPKPRMHE ACLLQRCHKP KKLQWLVSAW SQVGALVSRE RG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, expression analysis, and structural characterization of seven novel human ADAMTSs, a family of metalloproteinases with disintegrin and thrombospondin-1 domains." Cal S., Obaya A.J., Llamazares M., Garabaya C., Quesada V., Lopez-Otin C. Gene 283:49-62(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]	NHLBI resequencing and genotyping service (RS&G) Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The DNA sequence and comparative analysis of human chromosome 5." Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M. Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The nucleotide sequence of a long cDNA clone isolated from human." Nagase T., Kikuno R., Ohara O. Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 204-1224 (ISOFORM 1). Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ315734 mRNA. Translation: CAC86015.1. FJ515844 Genomic DNA. Translation: ACS13738.1. AC010269 Genomic DNA. No translation available. AC022424 Genomic DNA. No translation available. AC091978 Genomic DNA. No translation available. AB095949 mRNA. Translation: BAC23125.1.
IPI	IPI00186114. IPI00386697.
RefSeq	NP_620687.2. NM_139056.2.
UniGene	Hs.661915.
3D structure databases
ProteinModelPortal	Q8TE57.
SMR	Q8TE57. Positions 292-860, 866-1050.
ModBase	Search...
Protein family/group databases
MEROPS	M12.026.
PTM databases
PhosphoSite	Q8TE57.
Polymorphism databases
DMDM	296439429.
Proteomic databases
PaxDb	Q8TE57.
PRIDE	Q8TE57.
Protocols and materials databases
DNASU	170690.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000274181; ENSP00000274181; ENSG00000145536.
GeneID	170690.
KEGG	hsa:170690.
UCSC	uc003jdk.1. human. uc003jdl.3. human.
Organism-specific databases
CTD	170690.
GeneCards	GC05P005140.
HGNC	HGNC:17108. ADAMTS16.
MIM	607510. gene.
neXtProt	NX_Q8TE57.
PharmGKB	PA24542.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG237764.
HOGENOM	HOG000004800.
HOVERGEN	HBG004315.
InParanoid	Q8TE57.
KO	K08630.
OMA	EDQDLCK.
OrthoDB	EOG4DNF3K.
PhylomeDB	Q8TE57.
Gene expression databases
ArrayExpress	Q8TE57.
Bgee	Q8TE57.
CleanEx	HS_ADAMTS16.
Genevestigator	Q8TE57.
GermOnline	ENSG00000145536. Homo sapiens.
Family and domain databases
Gene3D	3.40.390.10. 1 hit.
InterPro	IPR010294. ADAM_spacer1. IPR024079. MetalloPept_cat_dom. IPR001590. Peptidase_M12B. IPR013273. Peptidase_M12B_ADAM-TS. IPR002870. Peptidase_M12B_N. IPR010909. PLAC. IPR000884. Thrombospondin_1_rpt. [Graphical view]
Pfam	PF05986. ADAM_spacer1. 1 hit. PF01562. Pep_M12B_propep. 1 hit. PF08686. PLAC. 1 hit. PF01421. Reprolysin. 1 hit. PF00090. TSP_1. 5 hits. [Graphical view]
PRINTS	PR01857. ADAMTSFAMILY.
SMART	SM00209. TSP1. 6 hits. [Graphical view]
SUPFAM	SSF82895. TSP1. 5 hits.
PROSITE	PS50215. ADAM_MEPRO. 1 hit. PS00546. CYSTEINE_SWITCH. False negative. PS00427. DISINTEGRIN_1. False negative. PS50214. DISINTEGRIN_2. False negative. PS50900. PLAC. 1 hit. PS50092. TSP1. 5 hits. PS00142. ZINC_PROTEASE. False negative. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	170690.
NextBio	89102.
SOURCE	Search...

Entry information

Entry name

ATS16_HUMAN

Accession

Primary (citable) accession number: Q8TE57
Secondary accession number(s): C6G490, Q8IVE2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2003
Last sequence update:	May 18, 2010
Last modified:	May 1, 2013
This is version 102 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.