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Protein

A disintegrin and metalloproteinase with thrombospondin motifs 16

Gene

ADAMTS16

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi249Zinc; in inhibited formBy similarity1
Metal bindingi433Zinc; catalyticPROSITE-ProRule annotation1
Active sitei434PROSITE-ProRule annotation1
Metal bindingi437Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi443Zinc; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-1474228 Degradation of the extracellular matrix
R-HSA-5083635 Defective B3GALTL causes Peters-plus syndrome (PpS)
R-HSA-5173214 O-glycosylation of TSR domain-containing proteins

Protein family/group databases

MEROPSiM12.026

Names & Taxonomyi

Protein namesi
Recommended name:
A disintegrin and metalloproteinase with thrombospondin motifs 16 (EC:3.4.24.-)
Short name:
ADAM-TS 16
Short name:
ADAM-TS16
Short name:
ADAMTS-16
Gene namesi
Name:ADAMTS16
Synonyms:KIAA2029
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000145536.15
HGNCiHGNC:17108 ADAMTS16
MIMi607510 gene
neXtProtiNX_Q8TE57

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Extracellular matrix, Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi170690
OpenTargetsiENSG00000145536
PharmGKBiPA24542

Polymorphism and mutation databases

BioMutaiADAMTS16
DMDMi296439429

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
PropeptideiPRO_000002919425 – 279By similarityAdd BLAST255
ChainiPRO_0000029195280 – 1224A disintegrin and metalloproteinase with thrombospondin motifs 16Add BLAST945

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi156N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi310N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi366 ↔ 417By similarity
Disulfide bondi392 ↔ 399By similarity
Disulfide bondi411 ↔ 490By similarity
Disulfide bondi450 ↔ 474By similarity
Disulfide bondi518 ↔ 543By similarity
Disulfide bondi529 ↔ 550By similarity
Disulfide bondi538 ↔ 569By similarity
Disulfide bondi563 ↔ 574By similarity
Disulfide bondi598 ↔ 635By similarity
Disulfide bondi602 ↔ 640By similarity
Disulfide bondi613 ↔ 625By similarity
Glycosylationi741N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi780N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi835N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi905N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi935N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi939 ↔ 981By similarity
Disulfide bondi943 ↔ 986By similarity
Disulfide bondi954 ↔ 970By similarity

Post-translational modificationi

The precursor is cleaved by a furin endopeptidase.By similarity
Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X2-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion (By similarity).By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ8TE57
PeptideAtlasiQ8TE57
PRIDEiQ8TE57

PTM databases

iPTMnetiQ8TE57
PhosphoSitePlusiQ8TE57

Expressioni

Tissue specificityi

Expressed in fetal lung and kidney and in adult prostate and ovary.

Gene expression databases

BgeeiENSG00000145536
CleanExiHS_ADAMTS16
ExpressionAtlasiQ8TE57 baseline and differential
GenevisibleiQ8TE57 HS

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000274181

Structurei

3D structure databases

ProteinModelPortaliQ8TE57
SMRiQ8TE57
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini290 – 495Peptidase M12BPROSITE-ProRule annotationAdd BLAST206
Domaini496 – 585DisintegrinAdd BLAST90
Domaini586 – 641TSP type-1 1PROSITE-ProRule annotationAdd BLAST56
Domaini874 – 922TSP type-1 2PROSITE-ProRule annotationAdd BLAST49
Domaini927 – 987TSP type-1 3PROSITE-ProRule annotationAdd BLAST61
Domaini988 – 1048TSP type-1 4PROSITE-ProRule annotationAdd BLAST61
Domaini1051 – 1115TSP type-1 5PROSITE-ProRule annotationAdd BLAST65
Domaini1127 – 1181TSP type-1 6PROSITE-ProRule annotationAdd BLAST55
Domaini1186 – 1223PLACPROSITE-ProRule annotationAdd BLAST38

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni747 – 873SpacerAdd BLAST127

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi247 – 254Cysteine switchBy similarity8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi642 – 746Cys-richAdd BLAST105

Domaini

The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.By similarity
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG3538 Eukaryota
ENOG410XPKZ LUCA
GeneTreeiENSGT00900000140815
HOGENOMiHOG000004800
HOVERGENiHBG004315
InParanoidiQ8TE57
KOiK08630
OMAiKKLQWLV
OrthoDBiEOG091G14M8
PhylomeDBiQ8TE57
TreeFamiTF313537

Family and domain databases

Gene3Di2.20.100.10, 5 hits
3.40.390.10, 1 hit
InterProiView protein in InterPro
IPR010294 ADAM_spacer1
IPR024079 MetalloPept_cat_dom_sf
IPR001590 Peptidase_M12B
IPR002870 Peptidase_M12B_N
IPR010909 PLAC
IPR000884 TSP1_rpt
IPR036383 TSP1_rpt_sf
PfamiView protein in Pfam
PF05986 ADAM_spacer1, 1 hit
PF01562 Pep_M12B_propep, 1 hit
PF08686 PLAC, 1 hit
PF01421 Reprolysin, 1 hit
PF00090 TSP_1, 5 hits
SMARTiView protein in SMART
SM00209 TSP1, 6 hits
SUPFAMiSSF82895 SSF82895, 5 hits
PROSITEiView protein in PROSITE
PS50215 ADAM_MEPRO, 1 hit
PS50900 PLAC, 1 hit
PS50092 TSP1, 5 hits

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8TE57-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKPRARGWRG LAALWMLLAQ VAEQAPACAM GPAAAAPGSP SVPRPPPPAE
60 70 80 90 100
RPGWMEKGEY DLVSAYEVDH RGDYVSHEIM HHQRRRRAVP VSEVESLHLR
110 120 130 140 150
LKGSRHDFHM DLRTSSSLVA PGFIVQTLGK TGTKSVQTLP PEDFCFYQGS
160 170 180 190 200
LRSHRNSSVA LSTCQGLSGM IRTEEADYFL RPLPSHLSWK LGRAAQGSSP
210 220 230 240 250
SHVLYKRSTE PHAPGASEVL VTSRTWELAH QPLHSSDLRL GLPQKQHFCG
260 270 280 290 300
RRKKYMPQPP KEDLFILPDE YKSCLRHKRS LLRSHRNEEL NVETLVVVDK
310 320 330 340 350
KMMQNHGHEN ITTYVLTILN MVSALFKDGT IGGNINIAIV GLILLEDEQP
360 370 380 390 400
GLVISHHADH TLSSFCQWQS GLMGKDGTRH DHAILLTGLD ICSWKNEPCD
410 420 430 440 450
TLGFAPISGM CSKYRSCTIN EDTGLGLAFT IAHESGHNFG MIHDGEGNMC
460 470 480 490 500
KKSEGNIMSP TLAGRNGVFS WSPCSRQYLH KFLSTAQAIC LADQPKPVKE
510 520 530 540 550
YKYPEKLPGE LYDANTQCKW QFGEKAKLCM LDFKKDICKA LWCHRIGRKC
560 570 580 590 600
ETKFMPAAEG TICGHDMWCR GGQCVKYGDE GPKPTHGHWS DWSSWSPCSR
610 620 630 640 650
TCGGGVSHRS RLCTNPKPSH GGKFCEGSTR TLKLCNSQKC PRDSVDFRAA
660 670 680 690 700
QCAEHNSRRF RGRHYKWKPY TQVEDQDLCK LYCIAEGFDF FFSLSNKVKD
710 720 730 740 750
GTPCSEDSRN VCIDGICERV GCDNVLGSDA VEDVCGVCNG NNSACTIHRG
760 770 780 790 800
LYTKHHHTNQ YYHMVTIPSG ARSIRIYEMN VSTSYISVRN ALRRYYLNGH
810 820 830 840 850
WTVDWPGRYK FSGTTFDYRR SYNEPENLIA TGPTNETLIV ELLFQGRNPG
860 870 880 890 900
VAWEYSMPRL GTEKQPPAQP SYTWAIVRSE CSVSCGGGQM TVREGCYRDL
910 920 930 940 950
KFQVNMSFCN PKTRPVTGLV PCKVSACPPS WSVGNWSACS RTCGGGAQSR
960 970 980 990 1000
PVQCTRRVHY DSEPVPASLC PQPAPSSRQA CNSQSCPPAW SAGPWAECSH
1010 1020 1030 1040 1050
TCGKGWRKRA VACKSTNPSA RAQLLPDAVC TSEPKPRMHE ACLLQRCHKP
1060 1070 1080 1090 1100
KKLQWLVSAW SQCSVTCERG TQKRFLKCAE KYVSGKYREL ASKKCSHLPK
1110 1120 1130 1140 1150
PSLELERACA PLPCPRHPPF AAAGPSRGSW FASPWSQCTA SCGGGVQTRS
1160 1170 1180 1190 1200
VQCLAGGRPA SGCLLHQKPS ASLACNTHFC PIAEKKDAFC KDYFHWCYLV
1210 1220
PQHGMCSHKF YGKQCCKTCS KSNL
Length:1,224
Mass (Da):136,203
Last modified:May 18, 2010 - v3
Checksum:iFB6115E6C97A9683
GO
Isoform 2 (identifier: Q8TE57-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1063-1072: CSVTCERGTQ → VGALVSRERG
     1073-1224: Missing.

Show »
Length:1,072
Mass (Da):119,507
Checksum:i3E2C0FF34194E631
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti90P → A in CAC86015 (PubMed:11867212).Curated1
Sequence conflicti104S → P in CAC86015 (PubMed:11867212).Curated1
Sequence conflicti284S → Y in BAC23125 (Ref. 4) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_057076110M → V. Corresponds to variant dbSNP:rs1863968Ensembl.1
Natural variantiVAR_057077486A → T. Corresponds to variant dbSNP:rs16875054Ensembl.1
Natural variantiVAR_057078789R → C. Corresponds to variant dbSNP:rs9313105Ensembl.1
Natural variantiVAR_057079859R → L. Corresponds to variant dbSNP:rs16875122Ensembl.1
Natural variantiVAR_057080863E → K. Corresponds to variant dbSNP:rs35394775Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0076641063 – 1072CSVTCERGTQ → VGALVSRERG in isoform 2. 1 Publication10
Alternative sequenceiVSP_0076651073 – 1224Missing in isoform 2. 1 PublicationAdd BLAST152

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ315734 mRNA Translation: CAC86015.1
FJ515844 Genomic DNA Translation: ACS13738.1
AC010269 Genomic DNA No translation available.
AC022424 Genomic DNA No translation available.
AC091978 Genomic DNA No translation available.
AB095949 mRNA Translation: BAC23125.1
CCDSiCCDS43299.1 [Q8TE57-1]
RefSeqiNP_620687.2, NM_139056.3 [Q8TE57-1]
UniGeneiHs.661915

Genome annotation databases

EnsembliENST00000274181; ENSP00000274181; ENSG00000145536 [Q8TE57-1]
GeneIDi170690
KEGGihsa:170690
UCSCiuc003jdl.4 human [Q8TE57-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiATS16_HUMAN
AccessioniPrimary (citable) accession number: Q8TE57
Secondary accession number(s): C6G490, Q8IVE2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 27, 2003
Last sequence update: May 18, 2010
Last modified: March 28, 2018
This is version 144 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health