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Reviewed, UniProtKB/Swiss-Prot Q8TE57 (ATS16_HUMAN)

Last modified November 24, 2009. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    A disintegrin and metalloproteinase with thrombospondin motifs 16
      Short name=ADAMTS-16
      Short name=ADAM-TS 16
      Short name=ADAM-TS16
    EC=3.4.24.-
Gene names
Name: ADAMTS16
Synonyms: KIAA2029
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length1224 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Cofactor

Binds 1 zinc ion per subunit By similarity.

Subcellular location

Secreted › extracellular space › extracellular matrix By similarity.

Tissue specificity

Expressed in fetal lung and kidney and in adult prostate and ovary.

Domain

The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix By similarity.

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Post-translational modification

The precursor is cleaved by a furin endopeptidase By similarity.

Sequence similarities

Contains 1 disintegrin domain.

Contains 1 peptidase M12B domain.

Contains 1 PLAC domain.

Contains 6 TSP type-1 domains.

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Ontologies

Keywords
   Cellular componentExtracellular matrix
Secreted
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainRepeat
Signal
   LigandMetal-binding
Zinc
   Molecular functionHydrolase
Metalloprotease
Protease
   PTMCleavage on pair of basic residues
Disulfide bond
Glycoprotein
Phosphoprotein
Zymogen
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processproteolysis

Inferred from electronic annotation. Source: InterPro

   Cellular componentproteinaceous extracellular matrix

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionmetalloendopeptidase activity

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8TE57-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8TE57-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1063-1072: CSVTCERGTQ → VGALVSRERG
     1073-1224: Missing.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Propeptide25 – 279255 By similarity
PRO_0000029194
Chain280 – 1224945A disintegrin and metalloproteinase with thrombospondin motifs 16
PRO_0000029195

Regions

Domain290 – 495206Peptidase M12B
Domain496 – 58590Disintegrin
Domain586 – 64156TSP type-1 1
Domain874 – 92249TSP type-1 2
Domain927 – 98761TSP type-1 3
Domain988 – 104861TSP type-1 4
Domain1051 – 111565TSP type-1 5
Domain1127 – 118155TSP type-1 6
Domain1186 – 122338PLAC
Region747 – 873127Spacer
Motif247 – 2548Cysteine switch By similarity
Compositional bias642 – 746105Cys-rich

Sites

Active site4341 By similarity
Metal binding2491Zinc; in inhibited form By similarity
Metal binding4331Zinc; catalytic By similarity
Metal binding4371Zinc; catalytic By similarity
Metal binding4431Zinc; catalytic By similarity

Amino acid modifications

Modified residue10961Phosphoserine Ref.3
Glycosylation1561N-linked (GlcNAc...) Potential
Glycosylation3101N-linked (GlcNAc...) Potential
Glycosylation7411N-linked (GlcNAc...) Potential
Glycosylation7801N-linked (GlcNAc...) Potential
Glycosylation8351N-linked (GlcNAc...) Potential
Glycosylation9051N-linked (GlcNAc...) Potential
Glycosylation9351N-linked (GlcNAc...) Potential
Disulfide bond411 ↔ 490 By similarity
Disulfide bond450 ↔ 474 By similarity
Disulfide bond598 ↔ 635 By similarity
Disulfide bond602 ↔ 640 By similarity
Disulfide bond613 ↔ 625 By similarity
Disulfide bond939 ↔ 981 By similarity
Disulfide bond943 ↔ 986 By similarity
Disulfide bond954 ↔ 970 By similarity

Natural variations

Alternative sequence1063 – 107210CSVTCERGTQ → VGALVSRERG in isoform 2.
VSP_007664
Alternative sequence1073 – 1224152Missing in isoform 2.
VSP_007665
Natural variant1101M → V: dbSNP rs1863968.
VAR_057076
Natural variant4861A → T: dbSNP rs16875054.
VAR_057077
Natural variant7891R → C: dbSNP rs9313105.
VAR_057078
Natural variant8591R → L: dbSNP rs16875122.
VAR_057079
Natural variant8631E → K: dbSNP rs35394775.
VAR_057080

Experimental info

Sequence conflict2841S → Y in BAC23125. Ref.2

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Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 27, 2003. Version 2.
Checksum: 572E72129E1401F8

FASTA1,224136,187
        10         20         30         40         50         60 
MKPRARGWRG LAALWMLLAQ VAEQAPACAM GPAAAAPGSP SVPRPPPPAE RPGWMEKGEY 

        70         80         90        100        110        120 
DLVSAYEVDH RGDYVSHEIM HHQRRRRAVA VSEVESLHLR LKGPRHDFHM DLRTSSSLVA 

       130        140        150        160        170        180 
PGFIVQTLGK TGTKSVQTLP PEDFCFYQGS LRSHRNSSVA LSTCQGLSGM IRTEEADYFL 

       190        200        210        220        230        240 
RPLPSHLSWK LGRAAQGSSP SHVLYKRSTE PHAPGASEVL VTSRTWELAH QPLHSSDLRL 

       250        260        270        280        290        300 
GLPQKQHFCG RRKKYMPQPP KEDLFILPDE YKSCLRHKRS LLRSHRNEEL NVETLVVVDK 

       310        320        330        340        350        360 
KMMQNHGHEN ITTYVLTILN MVSALFKDGT IGGNINIAIV GLILLEDEQP GLVISHHADH 

       370        380        390        400        410        420 
TLSSFCQWQS GLMGKDGTRH DHAILLTGLD ICSWKNEPCD TLGFAPISGM CSKYRSCTIN 

       430        440        450        460        470        480 
EDTGLGLAFT IAHESGHNFG MIHDGEGNMC KKSEGNIMSP TLAGRNGVFS WSPCSRQYLH 

       490        500        510        520        530        540 
KFLSTAQAIC LADQPKPVKE YKYPEKLPGE LYDANTQCKW QFGEKAKLCM LDFKKDICKA 

       550        560        570        580        590        600 
LWCHRIGRKC ETKFMPAAEG TICGHDMWCR GGQCVKYGDE GPKPTHGHWS DWSSWSPCSR 

       610        620        630        640        650        660 
TCGGGVSHRS RLCTNPKPSH GGKFCEGSTR TLKLCNSQKC PRDSVDFRAA QCAEHNSRRF 

       670        680        690        700        710        720 
RGRHYKWKPY TQVEDQDLCK LYCIAEGFDF FFSLSNKVKD GTPCSEDSRN VCIDGICERV 

       730        740        750        760        770        780 
GCDNVLGSDA VEDVCGVCNG NNSACTIHRG LYTKHHHTNQ YYHMVTIPSG ARSIRIYEMN 

       790        800        810        820        830        840 
VSTSYISVRN ALRRYYLNGH WTVDWPGRYK FSGTTFDYRR SYNEPENLIA TGPTNETLIV 

       850        860        870        880        890        900 
ELLFQGRNPG VAWEYSMPRL GTEKQPPAQP SYTWAIVRSE CSVSCGGGQM TVREGCYRDL 

       910        920        930        940        950        960 
KFQVNMSFCN PKTRPVTGLV PCKVSACPPS WSVGNWSACS RTCGGGAQSR PVQCTRRVHY 

       970        980        990       1000       1010       1020 
DSEPVPASLC PQPAPSSRQA CNSQSCPPAW SAGPWAECSH TCGKGWRKRA VACKSTNPSA 

      1030       1040       1050       1060       1070       1080 
RAQLLPDAVC TSEPKPRMHE ACLLQRCHKP KKLQWLVSAW SQCSVTCERG TQKRFLKCAE 

      1090       1100       1110       1120       1130       1140 
KYVSGKYREL ASKKCSHLPK PSLELERACA PLPCPRHPPF AAAGPSRGSW FASPWSQCTA 

      1150       1160       1170       1180       1190       1200 
SCGGGVQTRS VQCLAGGRPA SGCLLHQKPS ASLACNTHFC PIAEKKDAFC KDYFHWCYLV 

      1210       1220 
PQHGMCSHKF YGKQCCKTCS KSNL

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Isoform 2.

Checksum: 081EEFF78F47D061
Show »

FASTA1,072119,491

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References

« Hide 'large scale' references
[1]"Cloning, expression analysis, and structural characterization of seven novel human ADAMTSs, a family of metalloproteinases with disintegrin and thrombospondin-1 domains."
Cal S., Obaya A.J., Llamazares M., Garabaya C., Quesada V., Lopez-Otin C.
Gene 283:49-62(2002) [PubMed: 11867212] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]"The nucleotide sequence of a long cDNA clone isolated from human."
Nagase T., Kikuno R., Ohara O.
Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 204-1224 (ISOFORM 1).
Tissue: Brain.
[3]"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra."
Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D.
J. Proteome Res. 6:4150-4162(2007) [PubMed: 17924679] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1096, MASS SPECTROMETRY.
Tissue: Epithelium.
+Additional computationally mapped references.

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Cross-references

Sequence databases

AJ315734 mRNA. Translation: CAC86015.1.
AB095949 mRNA. Translation: BAC23125.1.
IPIIPI00186114.
IPI00386697.
RefSeqNP_620687.2.
UniGeneHs.661915

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGQ8TE57.

Protein family/group databases

MEROPSM12.026.

Genome annotation databases

EnsemblENST00000274181; ENSP00000274181; ENSG00000145536; Homo sapiens. [Genome view]
GeneID170690.
KEGGhsa:170690.
UCSCuc003jdl.1. human.

Organism-specific databases

CTD170690.
GeneCardsGC05P005193.
H-InvDBHIX0024784.
HGNCHGNC:17108. ADAMTS16.
MIM607510. gene.
PharmGKBPA24542.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

HOGENOMQ8TE57.
HOVERGENQ8TE57.

Gene expression databases

ArrayExpressQ8TE57.
BgeeQ8TE57.
CleanExHS_ADAMTS16.
GenevestigatorQ8TE57.
GermOnlineENSG00000145536. Homo sapiens.

Family and domain databases

InterProIPR010294. ADAM_spacer1.
IPR001590. Peptidase_M12B.
IPR013273. Peptidase_M12B_ADAM-TS.
IPR002870. Peptidase_M12B_N.
IPR010909. PLAC.
IPR000884. Thrombospondin_1_rpt.
[Graphical view]
PfamPF05986. ADAM_spacer1. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF08686. PLAC. 1 hit.
PF01421. Reprolysin. 1 hit.
PF00090. TSP_1. 5 hits.
[Graphical view]
PRINTSPR01857. ADAMTSFAMILY.
SMARTSM00209. TSP1. 6 hits.
[Graphical view]
PROSITEPS50215. ADAM_MEPRO. 1 hit.
PS00546. CYSTEINE_SWITCH. False negative.
PS00427. DISINTEGRIN_1. False negative.
PS50214. DISINTEGRIN_2. False negative.
PS50900. PLAC. 1 hit.
PS50092. TSP1. 5 hits.
PS00142. ZINC_PROTEASE. False negative.
[Graphical view]
ProtoNetSearch...

Other Resources

SOURCESearch...

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Entry information

Entry nameATS16_HUMAN
AccessionPrimary (citable) accession number: Q8TE57
Secondary accession number(s): Q8IVE2
Entry history
Integrated into UniProtKB/Swiss-Prot: June 27, 2003
Last sequence update: June 27, 2003
Last modified: November 24, 2009
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents