Q8TE49 (OTU7A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
OTU domain-containing protein 7A
EC=3.4.19.12

Alternative name(s):
Zinc finger protein Cezanne 2

Gene names

Name:	OTUD7A
Synonyms:	C15orf16, CEZANNE2, OTUD7

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	926 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has deubiquitinating activity that is directed towards 'Lys-48' or 'Lys-63'-linked polyubiquitin chains. Hydrolyzes both linear and branched forms of polyubiquitin By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the peptidase C64 family. Contains 1 A20-type zinc finger. Contains 1 OTU domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Protease Thiol protease
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8TE49-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8TE49-2)

The sequence of this isoform differs from the canonical sequence as follows:
     260-260: E → ESGLVYTE

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 926

926

OTU domain-containing protein 7A

PRO_0000188789

Regions

Domain

199 – 374

176

OTU

Zinc finger

884 – 919

36

A20-type

Region

168 – 410

243

TRAF-binding By similarity

Region

183 – 449

267

Catalytic By similarity

Motif

494 – 509

16

Nuclear localization signal Potential

Sites

Active site

210

1

Nucleophile By similarity

Active site

367

1

Proton acceptor By similarity

Natural variations

Alternative sequence

260

1

E → ESGLVYTE in isoform 2.

VSP_011430

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

926

Helix Strand Turn

Details...

Beta strand

13 – 15

3

Beta strand

22 – 24

3

Helix

27 – 38

12

Helix

42 – 51

10

Turn

52 – 54

3

Helix

56 – 68

13

Beta strand

71 – 75

5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 6E4623C2EB2C8058	FASTA	926	100,677
	10 20 30 40 50 60 MVSSVLPNPT SAECWAALLH DPMTLDMDAV LSDFVRSTGA EPGLARDLLE GKNWDLTAAL 70 80 90 100 110 120 SDYEQLRQVH TANLPHVFNE GRGPKQPERE PQPGHKVERP CLQRQDDIAQ EKRLSRGISH 130 140 150 160 170 180 ASSAIVSLAR SHVASECNNE QFPLEMPIYT FQLPDLSVYS EDFRSFIERD LIEQATMVAL 190 200 210 220 230 240 EQAGRLNWWS TVCTSCKRLL PLATTGDGNC LLHAASLGMW GFHDRDLVLR KALYTMMRTG 250 260 270 280 290 300 AEREALKRRW RWQQTQQNKE EEWEREWTEL LKLASSEPRT HFSKNGGTGG GVDNSEDPVY 310 320 330 340 350 360 ESLEEFHVFV LAHILRRPIV VVADTMLRDS GGEAFAPIPF GGIYLPLEVP PNRCHCSPLV 370 380 390 400 410 420 LAYDQAHFSA LVSMEQRDQQ REQAVIPLTD SEHKLLPLHF AVDPGKDWEW GKDDNDNARL 430 440 450 460 470 480 AHLILSLEAK LNLLHSYMNV TWIRIPSETR APLAQPESPT ASAGEDVQSL ADSLDSDRDS 490 500 510 520 530 540 VCSNSNSNNG KNGKDKEKEK QRKEKDKTRA DSVANKLGSF SKTLGIKLKK NMGGLGGLVH 550 560 570 580 590 600 GKMGRANSAN GKNGDSAERG KEKKAKSRKG SKEESGASAS TSPSEKTTPS PTDKAAGASP 610 620 630 640 650 660 AEKGGGPRGD AWKYSTDVKL SLNILRAAMQ GERKFIFAGL LLTSHRHQFH EEMIGYYLTS 670 680 690 700 710 720 AQERFSAEQE QRRRDAATAA AAAAAAAAAT AKRPPRRPET EGVPVPERAS PGPPTQLVLK 730 740 750 760 770 780 LKERPSPGPA AGRAARAAAG GTASPGGGAR RASASGPVPG RSPPAPARQS VIHVQASGAR 790 800 810 820 830 840 DEACAPAVGA LRPCATYPQQ NRSLSSQSYS PARAAALRTV NTVESLARAV PGALPGAAGT 850 860 870 880 890 900 AGAAEHKSQT YTNGFGALRD GLEFADADAP TARSNGECGR GGPGPVQRRC QRENCAFYGR 910 920 AETEHYCSYC YREELRRRRE ARGARP
	Isoform 2 [UniParc]. Checksum: D35018A59F8747FE	FASTA	933	101,427
	10 20 30 40 50 60 MVSSVLPNPT SAECWAALLH DPMTLDMDAV LSDFVRSTGA EPGLARDLLE GKNWDLTAAL 70 80 90 100 110 120 SDYEQLRQVH TANLPHVFNE GRGPKQPERE PQPGHKVERP CLQRQDDIAQ EKRLSRGISH 130 140 150 160 170 180 ASSAIVSLAR SHVASECNNE QFPLEMPIYT FQLPDLSVYS EDFRSFIERD LIEQATMVAL 190 200 210 220 230 240 EQAGRLNWWS TVCTSCKRLL PLATTGDGNC LLHAASLGMW GFHDRDLVLR KALYTMMRTG 250 260 270 280 290 300 AEREALKRRW RWQQTQQNKE SGLVYTEEEW EREWTELLKL ASSEPRTHFS KNGGTGGGVD 310 320 330 340 350 360 NSEDPVYESL EEFHVFVLAH ILRRPIVVVA DTMLRDSGGE AFAPIPFGGI YLPLEVPPNR 370 380 390 400 410 420 CHCSPLVLAY DQAHFSALVS MEQRDQQREQ AVIPLTDSEH KLLPLHFAVD PGKDWEWGKD 430 440 450 460 470 480 DNDNARLAHL ILSLEAKLNL LHSYMNVTWI RIPSETRAPL AQPESPTASA GEDVQSLADS 490 500 510 520 530 540 LDSDRDSVCS NSNSNNGKNG KDKEKEKQRK EKDKTRADSV ANKLGSFSKT LGIKLKKNMG 550 560 570 580 590 600 GLGGLVHGKM GRANSANGKN GDSAERGKEK KAKSRKGSKE ESGASASTSP SEKTTPSPTD 610 620 630 640 650 660 KAAGASPAEK GGGPRGDAWK YSTDVKLSLN ILRAAMQGER KFIFAGLLLT SHRHQFHEEM 670 680 690 700 710 720 IGYYLTSAQE RFSAEQEQRR RDAATAAAAA AAAAAATAKR PPRRPETEGV PVPERASPGP 730 740 750 760 770 780 PTQLVLKLKE RPSPGPAAGR AARAAAGGTA SPGGGARRAS ASGPVPGRSP PAPARQSVIH 790 800 810 820 830 840 VQASGARDEA CAPAVGALRP CATYPQQNRS LSSQSYSPAR AAALRTVNTV ESLARAVPGA 850 860 870 880 890 900 LPGAAGTAGA AEHKSQTYTN GFGALRDGLE FADADAPTAR SNGECGRGGP GPVQRRCQRE 910 920 930 NCAFYGRAET EHYCSYCYRE ELRRRREARG ARP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of a novel human gene, Cezanne 2." Evans P.C., Coadwell W.J., Kilshaw P.J. Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-678 (ISOFORM 2). Tissue: Brain.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AJ430383 mRNA. Translation: CAD23047.1.
BC035668 mRNA. Translation: AAH35668.1.

IPI

IPI00152625.
IPI00455137.

RefSeq

NP_570971.1. NM_130901.1.

UniGene

Hs.355236.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2L2D	NMR	-	A	11-83	[»]

ProteinModelPortal

Q8TE49.

ModBase

Search...

Protein-protein interaction databases

STRING

9606.ENSP00000305926.

Protein family/group databases

MEROPS

C64.002.

PTM databases

PhosphoSite

Q8TE49.

Polymorphism databases

DMDM

51701344.

Proteomic databases

PaxDb

Q8TE49.

PRIDE

Q8TE49.

Protocols and materials databases

DNASU

161725.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000307050; ENSP00000305926; ENSG00000169918.
ENST00000382902; ENSP00000372358; ENSG00000169918.

GeneID

161725.

KEGG

hsa:161725.

UCSC

uc001zfq.3. human.
uc001zfr.3. human.

Organism-specific databases

CTD

161725.

GeneCards

GC15M031773.

HGNC

HGNC:20718. OTUD7A.

HPA

HPA044554.

MIM

612024. gene.

neXtProt

NX_Q8TE49.

PharmGKB

PA134877572.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG282016.

HOGENOM

HOG000048103.

HOVERGEN

HBG050904.

KO

K11860.

OMA

FNEGRGP.

OrthoDB

EOG4BRWK6.

Gene expression databases

ArrayExpress

Q8TE49.

Bgee

Q8TE49.

CleanEx

HS_OTUD7A.

Genevestigator

Q8TE49.

GermOnline

ENSG00000169918. Homo sapiens.

Family and domain databases

InterPro

IPR003323. OTU.
IPR009060. UBA-like.
IPR002653. Znf_A20.
[Graphical view]

Pfam

PF02338. OTU. 1 hit.
[Graphical view]

SUPFAM

SSF46934. UBA_like. 1 hit.

PROSITE

PS50802. OTU. 1 hit.
PS51036. ZF_A20. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q8TE49.

GenomeRNAi

161725.

NextBio

88096.

SOURCE

Search...

Entry information

Entry name

OTU7A_HUMAN

Accession

Primary (citable) accession number: Q8TE49
Secondary accession number(s): Q8IWK5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.