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Q8TE49

- OTU7A_HUMAN

UniProt

Q8TE49 - OTU7A_HUMAN

Protein

OTU domain-containing protein 7A

Gene

OTUD7A

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Has deubiquitinating activity towards 'Lys-11'-linked polyubiquitin chains.2 Publications

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei207 – 2071By similarity
    Active sitei210 – 2101NucleophileBy similarity
    Active sitei367 – 3671Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri884 – 91936A20-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. ubiquitin-specific protease activity Source: UniProtKB
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. protein K11-linked deubiquitination Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    MEROPSiC64.002.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    OTU domain-containing protein 7A (EC:3.4.19.12)
    Alternative name(s):
    Zinc finger protein Cezanne 2
    Gene namesi
    Name:OTUD7A
    Synonyms:C15orf16, CEZANNE2, OTUD7
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 15

    Organism-specific databases

    HGNCiHGNC:20718. OTUD7A.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134877572.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 926926OTU domain-containing protein 7APRO_0000188789Add
    BLAST

    Proteomic databases

    MaxQBiQ8TE49.
    PaxDbiQ8TE49.
    PRIDEiQ8TE49.

    PTM databases

    PhosphoSiteiQ8TE49.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8TE49.
    BgeeiQ8TE49.
    CleanExiHS_OTUD7A.
    GenevestigatoriQ8TE49.

    Organism-specific databases

    HPAiHPA044554.

    Interactioni

    Protein-protein interaction databases

    BioGridi127799. 1 interaction.
    STRINGi9606.ENSP00000305926.

    Structurei

    Secondary structure

    1
    926
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi13 – 153
    Beta strandi22 – 243
    Helixi27 – 3812
    Helixi42 – 5110
    Turni52 – 543
    Helixi56 – 6813
    Beta strandi71 – 755

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2L2DNMR-A11-83[»]
    ProteinModelPortaliQ8TE49.
    SMRiQ8TE49. Positions 11-83, 146-404.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8TE49.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini199 – 374176OTUPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni168 – 410243TRAF-bindingBy similarityAdd
    BLAST
    Regioni183 – 449267CatalyticBy similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi494 – 50916Nuclear localization signalSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C64 family.Curated
    Contains 1 A20-type zinc finger.PROSITE-ProRule annotation
    Contains 1 OTU domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri884 – 91936A20-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiNOG282016.
    HOGENOMiHOG000048103.
    HOVERGENiHBG050904.
    KOiK11860.
    OMAiKQPEREP.
    OrthoDBiEOG7JHM57.
    PhylomeDBiQ8TE49.
    TreeFamiTF323312.

    Family and domain databases

    InterProiIPR003323. OTU.
    IPR002653. Znf_A20.
    [Graphical view]
    PfamiPF02338. OTU. 1 hit.
    [Graphical view]
    PROSITEiPS50802. OTU. 1 hit.
    PS51036. ZF_A20. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8TE49-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MVSSVLPNPT SAECWAALLH DPMTLDMDAV LSDFVRSTGA EPGLARDLLE    50
    GKNWDLTAAL SDYEQLRQVH TANLPHVFNE GRGPKQPERE PQPGHKVERP 100
    CLQRQDDIAQ EKRLSRGISH ASSAIVSLAR SHVASECNNE QFPLEMPIYT 150
    FQLPDLSVYS EDFRSFIERD LIEQATMVAL EQAGRLNWWS TVCTSCKRLL 200
    PLATTGDGNC LLHAASLGMW GFHDRDLVLR KALYTMMRTG AEREALKRRW 250
    RWQQTQQNKE EEWEREWTEL LKLASSEPRT HFSKNGGTGG GVDNSEDPVY 300
    ESLEEFHVFV LAHILRRPIV VVADTMLRDS GGEAFAPIPF GGIYLPLEVP 350
    PNRCHCSPLV LAYDQAHFSA LVSMEQRDQQ REQAVIPLTD SEHKLLPLHF 400
    AVDPGKDWEW GKDDNDNARL AHLILSLEAK LNLLHSYMNV TWIRIPSETR 450
    APLAQPESPT ASAGEDVQSL ADSLDSDRDS VCSNSNSNNG KNGKDKEKEK 500
    QRKEKDKTRA DSVANKLGSF SKTLGIKLKK NMGGLGGLVH GKMGRANSAN 550
    GKNGDSAERG KEKKAKSRKG SKEESGASAS TSPSEKTTPS PTDKAAGASP 600
    AEKGGGPRGD AWKYSTDVKL SLNILRAAMQ GERKFIFAGL LLTSHRHQFH 650
    EEMIGYYLTS AQERFSAEQE QRRRDAATAA AAAAAAAAAT AKRPPRRPET 700
    EGVPVPERAS PGPPTQLVLK LKERPSPGPA AGRAARAAAG GTASPGGGAR 750
    RASASGPVPG RSPPAPARQS VIHVQASGAR DEACAPAVGA LRPCATYPQQ 800
    NRSLSSQSYS PARAAALRTV NTVESLARAV PGALPGAAGT AGAAEHKSQT 850
    YTNGFGALRD GLEFADADAP TARSNGECGR GGPGPVQRRC QRENCAFYGR 900
    AETEHYCSYC YREELRRRRE ARGARP 926
    Length:926
    Mass (Da):100,677
    Last modified:June 1, 2002 - v1
    Checksum:i6E4623C2EB2C8058
    GO
    Isoform 2 (identifier: Q8TE49-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         260-260: E → ESGLVYTE

    Note: No experimental confirmation available.

    Show »
    Length:933
    Mass (Da):101,427
    Checksum:iD35018A59F8747FE
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei260 – 2601E → ESGLVYTE in isoform 2. 1 PublicationVSP_011430

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ430383 mRNA. Translation: CAD23047.1.
    BC035668 mRNA. Translation: AAH35668.1.
    CCDSiCCDS10026.1. [Q8TE49-1]
    RefSeqiNP_570971.1. NM_130901.1. [Q8TE49-1]
    XP_006720476.1. XM_006720413.1. [Q8TE49-2]
    UniGeneiHs.355236.

    Genome annotation databases

    EnsembliENST00000307050; ENSP00000305926; ENSG00000169918. [Q8TE49-1]
    GeneIDi161725.
    KEGGihsa:161725.
    UCSCiuc001zfq.3. human. [Q8TE49-1]
    uc001zfr.3. human. [Q8TE49-2]

    Polymorphism databases

    DMDMi51701344.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ430383 mRNA. Translation: CAD23047.1 .
    BC035668 mRNA. Translation: AAH35668.1 .
    CCDSi CCDS10026.1. [Q8TE49-1 ]
    RefSeqi NP_570971.1. NM_130901.1. [Q8TE49-1 ]
    XP_006720476.1. XM_006720413.1. [Q8TE49-2 ]
    UniGenei Hs.355236.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2L2D NMR - A 11-83 [» ]
    ProteinModelPortali Q8TE49.
    SMRi Q8TE49. Positions 11-83, 146-404.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 127799. 1 interaction.
    STRINGi 9606.ENSP00000305926.

    Protein family/group databases

    MEROPSi C64.002.

    PTM databases

    PhosphoSitei Q8TE49.

    Polymorphism databases

    DMDMi 51701344.

    Proteomic databases

    MaxQBi Q8TE49.
    PaxDbi Q8TE49.
    PRIDEi Q8TE49.

    Protocols and materials databases

    DNASUi 161725.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000307050 ; ENSP00000305926 ; ENSG00000169918 . [Q8TE49-1 ]
    GeneIDi 161725.
    KEGGi hsa:161725.
    UCSCi uc001zfq.3. human. [Q8TE49-1 ]
    uc001zfr.3. human. [Q8TE49-2 ]

    Organism-specific databases

    CTDi 161725.
    GeneCardsi GC15M031773.
    HGNCi HGNC:20718. OTUD7A.
    HPAi HPA044554.
    MIMi 612024. gene.
    neXtProti NX_Q8TE49.
    PharmGKBi PA134877572.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG282016.
    HOGENOMi HOG000048103.
    HOVERGENi HBG050904.
    KOi K11860.
    OMAi KQPEREP.
    OrthoDBi EOG7JHM57.
    PhylomeDBi Q8TE49.
    TreeFami TF323312.

    Miscellaneous databases

    EvolutionaryTracei Q8TE49.
    GenomeRNAii 161725.
    NextBioi 88096.
    PROi Q8TE49.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8TE49.
    Bgeei Q8TE49.
    CleanExi HS_OTUD7A.
    Genevestigatori Q8TE49.

    Family and domain databases

    InterProi IPR003323. OTU.
    IPR002653. Znf_A20.
    [Graphical view ]
    Pfami PF02338. OTU. 1 hit.
    [Graphical view ]
    PROSITEi PS50802. OTU. 1 hit.
    PS51036. ZF_A20. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation of a novel human gene, Cezanne 2."
      Evans P.C., Coadwell W.J., Kilshaw P.J.
      Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-678 (ISOFORM 2).
      Tissue: Brain.
    3. "Lys11-linked ubiquitin chains adopt compact conformations and are preferentially hydrolyzed by the deubiquitinase Cezanne."
      Bremm A., Freund S.M., Komander D.
      Nat. Struct. Mol. Biol. 17:939-947(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. "OTU deubiquitinases reveal mechanisms of linkage specificity and enable ubiquitin chain restriction analysis."
      Mevissen T.E., Hospenthal M.K., Geurink P.P., Elliott P.R., Akutsu M., Arnaudo N., Ekkebus R., Kulathu Y., Wauer T., El Oualid F., Freund S.M., Ovaa H., Komander D.
      Cell 154:169-184(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY.
    5. "The amino-terminal UBA domain of OTUD7A."
      Structural genomics consortium (SGC)
      Submitted (FEB-2012) to the PDB data bank
      Cited for: STRUCTURE BY NMR OF 11-83.

    Entry informationi

    Entry nameiOTU7A_HUMAN
    AccessioniPrimary (citable) accession number: Q8TE49
    Secondary accession number(s): Q8IWK5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 31, 2004
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 15
      Human chromosome 15: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. Peptidase families
      Classification of peptidase families and list of entries
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3