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Q8TE49 (OTU7A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
OTU domain-containing protein 7A

EC=3.4.19.12
Alternative name(s):
Zinc finger protein Cezanne 2
Gene names
Name:OTUD7A
Synonyms:C15orf16, CEZANNE2, OTUD7
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length926 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has deubiquitinating activity towards 'Lys-11'-linked polyubiquitin chains. Ref.3 Ref.4

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). Ref.4

Subcellular location

Cytoplasm By similarity. Nucleus By similarity.

Sequence similarities

Belongs to the peptidase C64 family.

Contains 1 A20-type zinc finger.

Contains 1 OTU domain.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   Cellular componentCytoplasm
Nucleus
   Coding sequence diversityAlternative splicing
   DomainZinc-finger
   LigandMetal-binding
Zinc
   Molecular functionHydrolase
Protease
Thiol protease
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processprotein K11-linked deubiquitination

Inferred from direct assay Ref.4. Source: UniProtKB

proteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

ubiquitin-specific protease activity

Inferred from direct assay Ref.4. Source: UniProtKB

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8TE49-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8TE49-2)

The sequence of this isoform differs from the canonical sequence as follows:
     260-260: E → ESGLVYTE
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 926926OTU domain-containing protein 7A
PRO_0000188789

Regions

Domain199 – 374176OTU
Zinc finger884 – 91936A20-type
Region168 – 410243TRAF-binding By similarity
Region183 – 449267Catalytic By similarity
Motif494 – 50916Nuclear localization signal Potential

Sites

Active site2071 By similarity
Active site2101Nucleophile By similarity
Active site3671Proton acceptor By similarity

Natural variations

Alternative sequence2601E → ESGLVYTE in isoform 2.
VSP_011430

Secondary structure

.............. 926
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 6E4623C2EB2C8058

FASTA926100,677
        10         20         30         40         50         60 
MVSSVLPNPT SAECWAALLH DPMTLDMDAV LSDFVRSTGA EPGLARDLLE GKNWDLTAAL 

        70         80         90        100        110        120 
SDYEQLRQVH TANLPHVFNE GRGPKQPERE PQPGHKVERP CLQRQDDIAQ EKRLSRGISH 

       130        140        150        160        170        180 
ASSAIVSLAR SHVASECNNE QFPLEMPIYT FQLPDLSVYS EDFRSFIERD LIEQATMVAL 

       190        200        210        220        230        240 
EQAGRLNWWS TVCTSCKRLL PLATTGDGNC LLHAASLGMW GFHDRDLVLR KALYTMMRTG 

       250        260        270        280        290        300 
AEREALKRRW RWQQTQQNKE EEWEREWTEL LKLASSEPRT HFSKNGGTGG GVDNSEDPVY 

       310        320        330        340        350        360 
ESLEEFHVFV LAHILRRPIV VVADTMLRDS GGEAFAPIPF GGIYLPLEVP PNRCHCSPLV 

       370        380        390        400        410        420 
LAYDQAHFSA LVSMEQRDQQ REQAVIPLTD SEHKLLPLHF AVDPGKDWEW GKDDNDNARL 

       430        440        450        460        470        480 
AHLILSLEAK LNLLHSYMNV TWIRIPSETR APLAQPESPT ASAGEDVQSL ADSLDSDRDS 

       490        500        510        520        530        540 
VCSNSNSNNG KNGKDKEKEK QRKEKDKTRA DSVANKLGSF SKTLGIKLKK NMGGLGGLVH 

       550        560        570        580        590        600 
GKMGRANSAN GKNGDSAERG KEKKAKSRKG SKEESGASAS TSPSEKTTPS PTDKAAGASP 

       610        620        630        640        650        660 
AEKGGGPRGD AWKYSTDVKL SLNILRAAMQ GERKFIFAGL LLTSHRHQFH EEMIGYYLTS 

       670        680        690        700        710        720 
AQERFSAEQE QRRRDAATAA AAAAAAAAAT AKRPPRRPET EGVPVPERAS PGPPTQLVLK 

       730        740        750        760        770        780 
LKERPSPGPA AGRAARAAAG GTASPGGGAR RASASGPVPG RSPPAPARQS VIHVQASGAR 

       790        800        810        820        830        840 
DEACAPAVGA LRPCATYPQQ NRSLSSQSYS PARAAALRTV NTVESLARAV PGALPGAAGT 

       850        860        870        880        890        900 
AGAAEHKSQT YTNGFGALRD GLEFADADAP TARSNGECGR GGPGPVQRRC QRENCAFYGR 

       910        920 
AETEHYCSYC YREELRRRRE ARGARP 

« Hide

Isoform 2 [UniParc].

Checksum: D35018A59F8747FE
Show »

FASTA933101,427

References

« Hide 'large scale' references
[1]"Isolation of a novel human gene, Cezanne 2."
Evans P.C., Coadwell W.J., Kilshaw P.J.
Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-678 (ISOFORM 2).
Tissue: Brain.
[3]"Lys11-linked ubiquitin chains adopt compact conformations and are preferentially hydrolyzed by the deubiquitinase Cezanne."
Bremm A., Freund S.M., Komander D.
Nat. Struct. Mol. Biol. 17:939-947(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"OTU deubiquitinases reveal mechanisms of linkage specificity and enable ubiquitin chain restriction analysis."
Mevissen T.E., Hospenthal M.K., Geurink P.P., Elliott P.R., Akutsu M., Arnaudo N., Ekkebus R., Kulathu Y., Wauer T., El Oualid F., Freund S.M., Ovaa H., Komander D.
Cell 154:169-184(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY.
[5]"The amino-terminal UBA domain of OTUD7A."
Structural genomics consortium (SGC)
Submitted (FEB-2012) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 11-83.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ430383 mRNA. Translation: CAD23047.1.
BC035668 mRNA. Translation: AAH35668.1.
RefSeqNP_570971.1. NM_130901.1.
UniGeneHs.355236.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2L2DNMR-A11-83[»]
ProteinModelPortalQ8TE49.
SMRQ8TE49. Positions 11-83, 138-447, 889-921.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid127799. 1 interaction.
STRING9606.ENSP00000305926.

Protein family/group databases

MEROPSC64.002.

PTM databases

PhosphoSiteQ8TE49.

Polymorphism databases

DMDM51701344.

Proteomic databases

PaxDbQ8TE49.
PRIDEQ8TE49.

Protocols and materials databases

DNASU161725.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000307050; ENSP00000305926; ENSG00000169918. [Q8TE49-1]
ENST00000382902; ENSP00000372358; ENSG00000169918. [Q8TE49-2]
GeneID161725.
KEGGhsa:161725.
UCSCuc001zfq.3. human. [Q8TE49-1]
uc001zfr.3. human. [Q8TE49-2]

Organism-specific databases

CTD161725.
GeneCardsGC15M031773.
HGNCHGNC:20718. OTUD7A.
HPAHPA044554.
MIM612024. gene.
neXtProtNX_Q8TE49.
PharmGKBPA134877572.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG282016.
HOGENOMHOG000048103.
HOVERGENHBG050904.
KOK11860.
OMAKQPEREP.
OrthoDBEOG7JHM57.
PhylomeDBQ8TE49.
TreeFamTF323312.

Gene expression databases

ArrayExpressQ8TE49.
BgeeQ8TE49.
CleanExHS_OTUD7A.
GenevestigatorQ8TE49.

Family and domain databases

InterProIPR003323. OTU.
IPR002653. Znf_A20.
[Graphical view]
PfamPF02338. OTU. 1 hit.
[Graphical view]
PROSITEPS50802. OTU. 1 hit.
PS51036. ZF_A20. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ8TE49.
GenomeRNAi161725.
NextBio88096.
PROQ8TE49.
SOURCESearch...

Entry information

Entry nameOTU7A_HUMAN
AccessionPrimary (citable) accession number: Q8TE49
Secondary accession number(s): Q8IWK5
Entry history
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: June 1, 2002
Last modified: April 16, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 15

Human chromosome 15: entries, gene names and cross-references to MIM