Q8TE04 (PANK1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pantothenate kinase 1
Short name=hPanK
Short name=hPanK1
EC=2.7.1.33

Alternative name(s):
Pantothenic acid kinase 1

Gene names

Name:	PANK1
Synonyms:	PANK

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	598 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in the physiological regulation of the intracellular CoA concentration By similarity.
Catalytic activity	ATP + (R)-pantothenate = ADP + (R)-4'-phosphopantothenate.
Enzyme regulation	Regulated by feedback inhibition by CoA and its thioesters. Strongly inhibited by acetyl-CoA and by manyl-CoA and also inhibited by high concentration of non-esterified CoA (CoASH) By similarity.
Pathway	Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 1/5.
Subcellular location	Cytoplasm Ref.2.
Tissue specificity	Isoform 1 is expressed at high levels in brain, heart, kidney, liver, skeletal muscle and testis. Isoform 2 is detected at much lower levels in kidney, liver, brain and testis and is not detected in heart or skeletal muscle. Ref.2
Induction	Isoform 1 is induced by bezafibrate, a hypolipidemic drug which acts as an agonist of peroxisome proliferator activator receptor alpha (PPARA), while isoform 2 levels decrease slightly. Ref.2
Domain	The N-terminal extension, present in isoform 1 may be the regulatory domain.
Sequence similarities	Belongs to the type II pantothenate kinase family.
Sequence caution	The sequence AAH26296.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Coenzyme A biosynthesis
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	coenzyme A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW pantothenate metabolic process Traceable author statement. Source: Reactome
Cellular component	cytosol Traceable author statement. Source: Reactome nucleus Inferred from direct assay. Source: HPA
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW pantothenate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8TE04-1)

Also known as: PanK1-alpha; PANK1a;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8TE04-2)

Also known as: PANK1a; PanK1-beta;

The sequence of this isoform differs from the canonical sequence as follows:
     1-235: MLKLVGGGGG...MDSGRKNRPP → MKLINGKKQT

Isoform 3 (identifier: Q8TE04-3)

Also known as: PANK1b;

The sequence of this isoform differs from the canonical sequence as follows:
     1-235: MLKLVGGGGG...MDSGRKNRPP → MKLINGKKQT
     438-496: Missing.

Isoform 4 (identifier: Q8TE04-4)

Also known as: PanK1-gamma;

The sequence of this isoform differs from the canonical sequence as follows:
     1-198: Missing.
     199-235: PLLPELQPQPLLPQHDSPAKKCRLRRRMDSGRKNRPP → MAKSKHALLPFCHGMMQQEGLHQMQSLDLGLLSLQNS

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 598

598

Pantothenate kinase 1

PRO_0000161802

Natural variations

Alternative sequence

1 – 235

235

MLKLV…KNRPP → MKLINGKKQT in isoform 2 and isoform 3.

VSP_004520

Alternative sequence

1 – 198

198

Missing in isoform 4.

VSP_012823

Alternative sequence

199 – 235

37

PLLPE…KNRPP → MAKSKHALLPFCHGMMQQEG LHQMQSLDLGLLSLQNS in isoform 4.

VSP_012824

Alternative sequence

438 – 496

59

Missing in isoform 3.

VSP_004521

Experimental info

Sequence conflict

306

1

N → D in AAL86371. Ref.1

Secondary structure

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598

Helix Strand Turn

Details...

Beta strand

238 – 243

6

Beta strand

245 – 255

11

Helix

273 – 279

7

Beta strand

284 – 286

3

Helix

291 – 293

3

Beta strand

295 – 299

5

Beta strand

304 – 313

10

Helix

314 – 316

3

Helix

317 – 323

7

Beta strand

338 – 340

3

Turn

341 – 344

4

Helix

345 – 347

3

Helix

363 – 377

15

Beta strand

384 – 390

7

Turn

394 – 396

3

Beta strand

398 – 402

5

Beta strand

409 – 426

18

Beta strand

429 – 437

9

Helix

440 – 451

12

Helix

456 – 465

10

Helix

468 – 470

3

Beta strand

472 – 474

3

Helix

475 – 479

5

Helix

484 – 486

3

Beta strand

492 – 495

4

Turn

496 – 501

6

Helix

503 – 506

4

Helix

511 – 537

27

Beta strand

542 – 546

5

Helix

547 – 549

3

Beta strand

551 – 553

3

Helix

554 – 566

13

Turn

567 – 569

3

Beta strand

573 – 576

4

Turn

577 – 580

4

Helix

582 – 591

10

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (PanK1-alpha) (PANK1a) [UniParc]. Last modified January 17, 2003. Version 2. Checksum: 0A92115D5BEDFC4C	FASTA	598	64,339
	10 20 30 40 50 60 MLKLVGGGGG QDWACSVAGT SLGGEEAAFE VARPGDQGKA GGGSPGWGCA GIPDSAPGAG 70 80 90 100 110 120 VLQAGAVGPA RGGQGAEEVG ESAGGGEERR VRHPQAPALR LLNRKPQGGS GEIKTPENDL 130 140 150 160 170 180 QRGRLSRGPR TAPPAPGMGD RSGQQERSVP HSPGAPVGTS AAAVNGLLHN GFHPPPVQPP 190 200 210 220 230 240 HVCSRGPVGG SDAAPQRLPL LPELQPQPLL PQHDSPAKKC RLRRRMDSGR KNRPPFPWFG 250 260 270 280 290 300 MDIGGTLVKL VYFEPKDITA EEEQEEVENL KSIRKYLTSN TAYGKTGIRD VHLELKNLTM 310 320 330 340 350 360 CGRKGNLHFI RFPSCAMHRF IQMGSEKNFS SLHTTLCATG GGAFKFEEDF RMIADLQLHK 370 380 390 400 410 420 LDELDCLIQG LLYVDSVGFN GKPECYYFEN PTNPELCQKK PYCLDNPYPM LLVNMGSGVS 430 440 450 460 470 480 ILAVYSKDNY KRVTGTSLGG GTFLGLCCLL TGCETFEEAL EMAAKGDSTN VDKLVKDIYG 490 500 510 520 530 540 GDYERFGLQG SAVASSFGNM MSKEKRDSIS KEDLARATLV TITNNIGSIA RMCALNENID 550 560 570 580 590 RVVFVGNFLR INMVSMKLLA YAMDFWSKGQ LKALFLEHEG YFGAVGALLE LFKMTDDK
	Isoform 2 (PANK1a) (PanK1-beta) [UniParc]. Checksum: 6F7D73D40E15F0F5	FASTA	373	41,671
	10 20 30 40 50 60 MKLINGKKQT FPWFGMDIGG TLVKLVYFEP KDITAEEEQE EVENLKSIRK YLTSNTAYGK 70 80 90 100 110 120 TGIRDVHLEL KNLTMCGRKG NLHFIRFPSC AMHRFIQMGS EKNFSSLHTT LCATGGGAFK 130 140 150 160 170 180 FEEDFRMIAD LQLHKLDELD CLIQGLLYVD SVGFNGKPEC YYFENPTNPE LCQKKPYCLD 190 200 210 220 230 240 NPYPMLLVNM GSGVSILAVY SKDNYKRVTG TSLGGGTFLG LCCLLTGCET FEEALEMAAK 250 260 270 280 290 300 GDSTNVDKLV KDIYGGDYER FGLQGSAVAS SFGNMMSKEK RDSISKEDLA RATLVTITNN 310 320 330 340 350 360 IGSIARMCAL NENIDRVVFV GNFLRINMVS MKLLAYAMDF WSKGQLKALF LEHEGYFGAV 370 GALLELFKMT DDK
	Isoform 3 (PANK1b) [UniParc]. Checksum: 56D24B548DE056DE	FASTA	314	35,579
	10 20 30 40 50 60 MKLINGKKQT FPWFGMDIGG TLVKLVYFEP KDITAEEEQE EVENLKSIRK YLTSNTAYGK 70 80 90 100 110 120 TGIRDVHLEL KNLTMCGRKG NLHFIRFPSC AMHRFIQMGS EKNFSSLHTT LCATGGGAFK 130 140 150 160 170 180 FEEDFRMIAD LQLHKLDELD CLIQGLLYVD SVGFNGKPEC YYFENPTNPE LCQKKPYCLD 190 200 210 220 230 240 NPYPMLLVNM GSGVSILAVY SKDNYKRVTG TSFGNMMSKE KRDSISKEDL ARATLVTITN 250 260 270 280 290 300 NIGSIARMCA LNENIDRVVF VGNFLRINMV SMKLLAYAMD FWSKGQLKAL FLEHEGYFGA 310 VGALLELFKM TDDK
	Isoform 4 (PanK1-gamma) [UniParc]. Checksum: 9BA80EA526F43832	FASTA	400	44,634
	10 20 30 40 50 60 MAKSKHALLP FCHGMMQQEG LHQMQSLDLG LLSLQNSFPW FGMDIGGTLV KLVYFEPKDI 70 80 90 100 110 120 TAEEEQEEVE NLKSIRKYLT SNTAYGKTGI RDVHLELKNL TMCGRKGNLH FIRFPSCAMH 130 140 150 160 170 180 RFIQMGSEKN FSSLHTTLCA TGGGAFKFEE DFRMIADLQL HKLDELDCLI QGLLYVDSVG 190 200 210 220 230 240 FNGKPECYYF ENPTNPELCQ KKPYCLDNPY PMLLVNMGSG VSILAVYSKD NYKRVTGTSL 250 260 270 280 290 300 GGGTFLGLCC LLTGCETFEE ALEMAAKGDS TNVDKLVKDI YGGDYERFGL QGSAVASSFG 310 320 330 340 350 360 NMMSKEKRDS ISKEDLARAT LVTITNNIGS IARMCALNEN IDRVVFVGNF LRINMVSMKL 370 380 390 400 LAYAMDFWSK GQLKALFLEH EGYFGAVGAL LELFKMTDDK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of a novel human pantothenate kinase gene." Ni X., Ma Y., Cheng H., Jiang M., Ying K., Xie Y., Mao Y. Int. J. Biochem. Cell Biol. 34:109-115(2002) [PubMed: 11809413] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3). Tissue: Fetal brain.
[2]	"PPARalpha controls the intracellular coenzyme A concentration via regulation of PANK1alpha gene expression." Ramaswamy G., Karim M.A., Murti K.G., Jackowski S. J. Lipid Res. 45:17-31(2004) [PubMed: 14523052] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 4), SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
[3]	"The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J. Nature 429:375-381(2004) [PubMed: 15164054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 460-598 (ISOFORM 2). Tissue: Skeletal muscle.
[6]	"A novel pantothenate kinase gene (PANK2) is defective in Hallervorden-Spatz syndrome." Zhou B., Westaway S.K., Levinson B., Johnson M.A., Gitschier J., Hayflick S.J. Nat. Genet. 28:345-349(2001) [PubMed: 11479594] [Abstract] Cited for: IDENTIFICATION (ISOFORM 2).
[7]	Zhou B., Westaway S.K., Levinson B., Johnson M.A., Gitschier J., Hayflick S.J. Unpublished observations (JUL-2001) Cited for: IDENTIFICATION (ISOFORM 1).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF355198 mRNA. Translation: AAL86371.1.
AL157400 Genomic DNA. Translation: CAI16826.1.
AL157400 Genomic DNA. Translation: CAI16827.1.
AY027661 mRNA. Translation: AAK20916.1.
AY027662 mRNA. Translation: AAK20917.1.
AY027663 mRNA. Translation: AAK20918.1.
CH471066 Genomic DNA. Translation: EAW50131.1.
BC026296 mRNA. Translation: AAH26296.1. Different initiation.
BK000008 mRNA. Translation: DAA00002.1.
BK000009 mRNA. Translation: DAA00003.1.

IPI

IPI00152578.
IPI00170950.
IPI00220147.
IPI00553196.

RefSeq

NP_612189.2. NM_138316.3.
NP_683878.1. NM_148977.2.
NP_683879.1. NM_148978.2.

UniGene

Hs.376351.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2I7N	X-ray	1.90	A/B	234-593	[»]
3SMP	X-ray	1.90	A/B	231-597	[»]

ProteinModelPortal

Q8TE04.

SMR

Q8TE04. Positions 232-594.

ModBase

Search...

Protein-protein interaction databases

MINT

MINT-3319085.

STRING

Q8TE04.

PTM databases

PhosphoSite

Q8TE04.

Polymorphism databases

DMDM

27805665.

Proteomic databases

PRIDE

Q8TE04.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000307534; ENSP00000302108; ENSG00000152782.

GeneID

53354.

KEGG

hsa:53354.

NMPDR

fig|9606.3.peg.4324.

UCSC

uc001kgn.1. human.
uc001kgo.1. human.
uc001kgp.1. human.
uc009xtu.1. human.

Organism-specific databases

CTD

53354.

GeneCards

GC10M091332.

H-InvDB

HIX0009024.

HGNC

HGNC:8598. PANK1.

HPA

HPA021795.

MIM

606160. gene.

neXtProt

NX_Q8TE04.

PharmGKB

PA32928.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG16781.

GeneTree

ENSGT00390000020719.

HOGENOM

HBG736409.

HOVERGEN

HBG053495.

InParanoid

Q8TE04.

OMA

GFHPPPV.

OrthoDB

EOG49P9ZQ.

PhylomeDB

Q8TE04.

Enzyme and pathway databases

Reactome

REACT_111217. Metabolism.

Gene expression databases

ArrayExpress

Q8TE04.

Bgee

Q8TE04.

CleanEx

HS_PANK1.

Genevestigator

Q8TE04.

GermOnline

ENSG00000152782. Homo sapiens.

Family and domain databases

InterPro

IPR004567. Type_II_PanK.
[Graphical view]

KO

K09680.

Pfam

PF03630. Fumble. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00555. PanK_eukar. 1 hit.

ProtoNet

Search...

Other

DrugBank

DB01393. Bezafibrate.

NextBio

56014.

SOURCE

Search...

Entry information

Entry name

PANK1_HUMAN

Accession

Primary (citable) accession number: Q8TE04
Secondary accession number(s): A6NIP0 , Q7RTX6, Q7Z495, Q8TBQ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 17, 2003
Last sequence update:	January 17, 2003
Last modified:	January 25, 2012
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.