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Q8TDY4 (ASAP3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3
Alternative name(s):
Development and differentiation-enhancing factor-like 1
Protein up-regulated in liver cancer 1
Gene names
Name:ASAP3
Synonyms:DDEFL1, UPLC1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length903 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Promotes cell proliferation. Ref.1

Subcellular location

Cytoplasm Ref.1.

Tissue specificity

Highly expressed in primary hepatocarcinoma. Detected in lung, liver and blood leukocytes. Ref.1

Sequence similarities

Contains 2 ANK repeats.

Contains 1 Arf-GAP domain.

Contains 1 PH domain.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8TDY4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8TDY4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-496: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q8TDY4-3)

The sequence of this isoform differs from the canonical sequence as follows:
     142-158: DSKKQLEKAWKDYEAKM → ASLSLGSR
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 903903Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3
PRO_0000232887

Regions

Domain302 – 39493PH
Domain426 – 551126Arf-GAP
Repeat585 – 61733ANK 1
Repeat621 – 65030ANK 2
Zinc finger441 – 46424C4-type
Coiled coil141 – 17232 Potential
Coiled coil248 – 27528 Potential
Compositional bias798 – 8014Poly-Ser

Natural variations

Alternative sequence1 – 496496Missing in isoform 2.
VSP_018013
Alternative sequence142 – 15817DSKKQ…YEAKM → ASLSLGSR in isoform 3.
VSP_039827
Natural variant3771E → A.
Corresponds to variant rs16828486 [ dbSNP | Ensembl ].
VAR_048295
Natural variant6171A → T in a colorectal cancer sample; somatic mutation. Ref.7
VAR_035612

Experimental info

Sequence conflict3191V → A in AAH60786. Ref.5
Sequence conflict5211E → G in BAA91008. Ref.2
Sequence conflict5961V → D in BAA91008. Ref.2
Sequence conflict8021L → V in BAA91008. Ref.2

Secondary structure

................................................. 903
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: D5E89518AFC86656

FASTA90399,155
        10         20         30         40         50         60 
MPEQFSVAEF LAVTAEDLSS PAGAAAFAAK MPRYRGAALA REEILEGDQA ILQRIKKAVR 

        70         80         90        100        110        120 
AIHSSGLGHV ENEEQYREAV ESLGNSHLSQ NSHELSTGFL NLAVFTREVA ALFKNLIQNL 

       130        140        150        160        170        180 
NNIVSFPLDS LMKGQLRDGR QDSKKQLEKA WKDYEAKMAK LEKERDRARV TGGIPGEVAQ 

       190        200        210        220        230        240 
DMQRERRIFQ LHMCEYLLKA GESQMKQGPD FLQSLIKFFH AQHNFFQDGW KAAQSLFPFI 

       250        260        270        280        290        300 
EKLAASVHAL HQAQEDELQK LTQLRDSLRG TLQLESREEH LSRKNSGCGY SIHQHQGNKQ 

       310        320        330        340        350        360 
FGTEKVGFLY KKSDGIRRVW QKRKCGVKYG CLTISHSTIN RPPVKLTLLT CQVRPNPEEK 

       370        380        390        400        410        420 
KCFDLVTHNR TYHFQAEDEH ECEAWVSVLQ NSKDEALSSA FLGEPSAGPG SWGSAGHDGE 

       430        440        450        460        470        480 
PHDLTKLLIA EVKSRPGNSQ CCDCGAADPT WLSTNLGVLT CIQCSGVHRE LGVRFSRMQS 

       490        500        510        520        530        540 
LTLDLLGPSE LLLALNMGNT SFNEVMEAQL PSHGGPKPSA ESDMGTRRDY IMAKYVEHRF 

       550        560        570        580        590        600 
ARRCTPEPQR LWTAICNRDL LSVLEAFANG QDFGQPLPGP DAQAPEELVL HLAVKVANQA 

       610        620        630        640        650        660 
SLPLVDFIIQ NGGHLDAKAA DGNTALHYAA LYNQPDCLKL LLKGRALVGT VNEAGETALD 

       670        680        690        700        710        720 
IARKKHHKEC EELLEQAQAG TFAFPLHVDY SWVISTEPGS DSEEDEEEKR CLLKLPAQAH 

       730        740        750        760        770        780 
WASGRLDISN KTYETVASLG AATPQGESED CPPPLPVKNS SRTLVQGCAR HASGDRSEVS 

       790        800        810        820        830        840 
SLSSEAPETP ESLGSPASSS SLMSPLEPGD PSQAPPNSEE GLREPPGTSR PSLTSGTTPS 

       850        860        870        880        890        900 
EMYLPVRFSS ESTRSYRRGA RSPEDGPSAR QPLPRRNVPV GITEGDGSRT GSLPASSVQL 


LQD 

« Hide

Isoform 2 [UniParc].

Checksum: 563CCA514252A27A
Show »

FASTA40743,695
Isoform 3 [UniParc].

Checksum: 124043E01A5F8C85
Show »

FASTA89497,847

References

« Hide 'large scale' references
[1]"Isolation of development and differentiation enhancing factor-like 1 (DDEFL1) as a drug target for hepatocellular carcinomas."
Okabe H., Furukawa Y., Kato T., Hasegawa S., Yamaoka Y., Nakamura Y.
Int. J. Oncol. 24:43-48(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Tissue: Liver.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
Tissue: Brain, Colon mucosa and Tongue.
[3]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain and Testis.
[6]"Structural analysis of gap and ankyrin domains of UPLC1."
Structural genomics consortium (SGC)
Submitted (SEP-2005) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.06 ANGSTROMS) OF 416-697 IN COMPLEX WITH ZINC IONS.
[7]"The consensus coding sequences of human breast and colorectal cancers."
Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. expand/collapse author list , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.
Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] THR-617.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB056749 mRNA. Translation: BAB85677.1.
AK000206 mRNA. Translation: BAA91008.1.
AK092291 mRNA. Translation: BAG52522.1.
AK295100 mRNA. Translation: BAG58136.1.
AL021154, AL357134 Genomic DNA. Translation: CAI19596.1.
AL357134, AL021154 Genomic DNA. Translation: CAI22177.1.
CH471134 Genomic DNA. Translation: EAW95050.1.
BC052305 mRNA. Translation: AAH52305.1.
BC060786 mRNA. Translation: AAH60786.1.
CCDSCCDS235.1. [Q8TDY4-1]
CCDS44087.1. [Q8TDY4-3]
RefSeqNP_001137250.1. NM_001143778.1. [Q8TDY4-3]
NP_060177.2. NM_017707.3. [Q8TDY4-1]
UniGeneHs.437379.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2B0OX-ray2.06E/F/G/H416-697[»]
3LVQX-ray3.38E416-697[»]
3LVRX-ray3.38E416-697[»]
ProteinModelPortalQ8TDY4.
SMRQ8TDY4. Positions 304-408, 423-687.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120756. 2 interactions.
IntActQ8TDY4. 1 interaction.
STRING9606.ENSP00000338769.

PTM databases

PhosphoSiteQ8TDY4.

Polymorphism databases

DMDM74751433.

Proteomic databases

MaxQBQ8TDY4.
PaxDbQ8TDY4.
PeptideAtlasQ8TDY4.
PRIDEQ8TDY4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000336689; ENSP00000338769; ENSG00000088280. [Q8TDY4-1]
ENST00000437606; ENSP00000408826; ENSG00000088280. [Q8TDY4-3]
ENST00000495646; ENSP00000436150; ENSG00000088280. [Q8TDY4-2]
GeneID55616.
KEGGhsa:55616.
UCSCuc001bgy.1. human. [Q8TDY4-2]
uc001bha.2. human. [Q8TDY4-1]
uc010oea.1. human. [Q8TDY4-3]

Organism-specific databases

CTD55616.
GeneCardsGC01M023755.
H-InvDBHIX0000247.
HGNCHGNC:14987. ASAP3.
HPAHPA020537.
HPA020546.
neXtProtNX_Q8TDY4.
PharmGKBPA164716179.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5347.
HOGENOMHOG000230570.
HOVERGENHBG051327.
InParanoidQ8TDY4.
KOK12488.
OMAEHECEAW.
OrthoDBEOG7WQ7RJ.
PhylomeDBQ8TDY4.
TreeFamTF325156.

Gene expression databases

ArrayExpressQ8TDY4.
BgeeQ8TDY4.
CleanExHS_ASAP3.
GenevestigatorQ8TDY4.

Family and domain databases

Gene3D1.20.1270.60. 1 hit.
1.25.40.20. 1 hit.
2.30.29.30. 1 hit.
InterProIPR027267. AH/BAR-dom.
IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001164. ArfGAP.
IPR028775. ASAP3.
IPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
[Graphical view]
PANTHERPTHR23180:SF211. PTHR23180:SF211. 1 hit.
PfamPF12796. Ank_2. 1 hit.
PF01412. ArfGap. 1 hit.
PF00169. PH. 1 hit.
[Graphical view]
PRINTSPR00405. REVINTRACTNG.
SMARTSM00248. ANK. 2 hits.
SM00105. ArfGap. 1 hit.
SM00233. PH. 1 hit.
[Graphical view]
SUPFAMSSF48403. SSF48403. 1 hit.
PROSITEPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS50115. ARFGAP. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSASAP3. human.
EvolutionaryTraceQ8TDY4.
GenomeRNAi55616.
NextBio60208.
PROQ8TDY4.

Entry information

Entry nameASAP3_HUMAN
AccessionPrimary (citable) accession number: Q8TDY4
Secondary accession number(s): B3KRW0 expand/collapse secondary AC list , B4DHH4, Q6P9F4, Q86UY1, Q9NXK2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: June 1, 2002
Last modified: July 9, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM