Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3

Gene

ASAP3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Promotes cell proliferation.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri441 – 464C4-typePROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

  • GTPase activator activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • cell migration Source: UniProtKB
  • positive regulation of GTPase activity Source: UniProtKB
  • regulation of stress fiber assembly Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:ENSG00000088280-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3
Alternative name(s):
Development and differentiation-enhancing factor-like 1
Protein up-regulated in liver cancer 1
Gene namesi
Name:ASAP3
Synonyms:DDEFL1, UPLC1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:14987. ASAP3.

Subcellular locationi

  • Cytoplasm 1 Publication

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • focal adhesion Source: UniProtKB
  • ruffle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi55616.
OpenTargetsiENSG00000088280.
ENSG00000282854.
PharmGKBiPA164716179.

Polymorphism and mutation databases

BioMutaiASAP3.
DMDMi74751433.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002328871 – 903Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3Add BLAST903

Proteomic databases

MaxQBiQ8TDY4.
PaxDbiQ8TDY4.
PeptideAtlasiQ8TDY4.
PRIDEiQ8TDY4.

PTM databases

iPTMnetiQ8TDY4.
PhosphoSitePlusiQ8TDY4.

Expressioni

Tissue specificityi

Highly expressed in primary hepatocarcinoma. Detected in lung, liver and blood leukocytes.1 Publication

Gene expression databases

BgeeiENSG00000088280.
CleanExiHS_ASAP3.
ExpressionAtlasiQ8TDY4. baseline and differential.
GenevisibleiQ8TDY4. HS.

Organism-specific databases

HPAiHPA020537.
HPA020546.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
PDZD3Q86UT53EBI-2609717,EBI-8744528
RELQ048643EBI-2609717,EBI-307352
TFCP2Q128003EBI-2609717,EBI-717422
UBE3AQ05086-23EBI-2609717,EBI-10175863

Protein-protein interaction databases

BioGridi120756. 15 interactors.
IntActiQ8TDY4. 7 interactors.
STRINGi9606.ENSP00000338769.

Structurei

Secondary structure

1903
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi424 – 433Combined sources10
Turni436 – 439Combined sources4
Turni442 – 444Combined sources3
Beta strandi451 – 453Combined sources3
Turni454 – 456Combined sources3
Helixi462 – 471Combined sources10
Turni473 – 475Combined sources3
Beta strandi478 – 480Combined sources3
Turni481 – 483Combined sources3
Helixi488 – 496Combined sources9
Helixi499 – 506Combined sources8
Turni507 – 509Combined sources3
Beta strandi512 – 514Combined sources3
Helixi524 – 535Combined sources12
Helixi548 – 556Combined sources9
Helixi560 – 568Combined sources9
Beta strandi573 – 575Combined sources3
Beta strandi580 – 582Combined sources3
Helixi589 – 595Combined sources7
Turni599 – 601Combined sources3
Helixi602 – 611Combined sources10
Beta strandi620 – 622Combined sources3
Helixi625 – 631Combined sources7
Helixi635 – 643Combined sources9
Helixi658 – 665Combined sources8
Helixi668 – 680Combined sources13
Helixi690 – 696Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2B0OX-ray2.06E/F/G/H416-697[»]
3LVQX-ray3.38E416-697[»]
3LVRX-ray3.38E416-697[»]
ProteinModelPortaliQ8TDY4.
SMRiQ8TDY4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8TDY4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini302 – 394PHPROSITE-ProRule annotationAdd BLAST93
Domaini426 – 551Arf-GAPPROSITE-ProRule annotationAdd BLAST126
Repeati585 – 617ANK 1Add BLAST33
Repeati621 – 650ANK 2Add BLAST30

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili141 – 172Sequence analysisAdd BLAST32
Coiled coili248 – 275Sequence analysisAdd BLAST28

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi798 – 801Poly-Ser4

Sequence similaritiesi

Contains 2 ANK repeats.PROSITE-ProRule annotation
Contains 1 Arf-GAP domain.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri441 – 464C4-typePROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

ANK repeat, Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0521. Eukaryota.
COG5347. LUCA.
GeneTreeiENSGT00760000118874.
HOGENOMiHOG000230570.
HOVERGENiHBG051327.
InParanoidiQ8TDY4.
KOiK12488.
OMAiIIQNGGH.
OrthoDBiEOG091G022H.
PhylomeDBiQ8TDY4.
TreeFamiTF325156.

Family and domain databases

Gene3Di1.20.1270.60. 1 hit.
1.25.40.20. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR027267. AH/BAR-dom.
IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001164. ArfGAP.
IPR028775. ASAP3.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PANTHERiPTHR23180:SF271. PTHR23180:SF271. 2 hits.
PfamiPF12796. Ank_2. 1 hit.
PF01412. ArfGap. 1 hit.
PF00169. PH. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00248. ANK. 2 hits.
SM00105. ArfGap. 1 hit.
SM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS50115. ARFGAP. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8TDY4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPEQFSVAEF LAVTAEDLSS PAGAAAFAAK MPRYRGAALA REEILEGDQA
60 70 80 90 100
ILQRIKKAVR AIHSSGLGHV ENEEQYREAV ESLGNSHLSQ NSHELSTGFL
110 120 130 140 150
NLAVFTREVA ALFKNLIQNL NNIVSFPLDS LMKGQLRDGR QDSKKQLEKA
160 170 180 190 200
WKDYEAKMAK LEKERDRARV TGGIPGEVAQ DMQRERRIFQ LHMCEYLLKA
210 220 230 240 250
GESQMKQGPD FLQSLIKFFH AQHNFFQDGW KAAQSLFPFI EKLAASVHAL
260 270 280 290 300
HQAQEDELQK LTQLRDSLRG TLQLESREEH LSRKNSGCGY SIHQHQGNKQ
310 320 330 340 350
FGTEKVGFLY KKSDGIRRVW QKRKCGVKYG CLTISHSTIN RPPVKLTLLT
360 370 380 390 400
CQVRPNPEEK KCFDLVTHNR TYHFQAEDEH ECEAWVSVLQ NSKDEALSSA
410 420 430 440 450
FLGEPSAGPG SWGSAGHDGE PHDLTKLLIA EVKSRPGNSQ CCDCGAADPT
460 470 480 490 500
WLSTNLGVLT CIQCSGVHRE LGVRFSRMQS LTLDLLGPSE LLLALNMGNT
510 520 530 540 550
SFNEVMEAQL PSHGGPKPSA ESDMGTRRDY IMAKYVEHRF ARRCTPEPQR
560 570 580 590 600
LWTAICNRDL LSVLEAFANG QDFGQPLPGP DAQAPEELVL HLAVKVANQA
610 620 630 640 650
SLPLVDFIIQ NGGHLDAKAA DGNTALHYAA LYNQPDCLKL LLKGRALVGT
660 670 680 690 700
VNEAGETALD IARKKHHKEC EELLEQAQAG TFAFPLHVDY SWVISTEPGS
710 720 730 740 750
DSEEDEEEKR CLLKLPAQAH WASGRLDISN KTYETVASLG AATPQGESED
760 770 780 790 800
CPPPLPVKNS SRTLVQGCAR HASGDRSEVS SLSSEAPETP ESLGSPASSS
810 820 830 840 850
SLMSPLEPGD PSQAPPNSEE GLREPPGTSR PSLTSGTTPS EMYLPVRFSS
860 870 880 890 900
ESTRSYRRGA RSPEDGPSAR QPLPRRNVPV GITEGDGSRT GSLPASSVQL

LQD
Length:903
Mass (Da):99,155
Last modified:June 1, 2002 - v1
Checksum:iD5E89518AFC86656
GO
Isoform 2 (identifier: Q8TDY4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-496: Missing.

Note: No experimental confirmation available.
Show »
Length:407
Mass (Da):43,695
Checksum:i563CCA514252A27A
GO
Isoform 3 (identifier: Q8TDY4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     142-158: DSKKQLEKAWKDYEAKM → ASLSLGSR

Note: No experimental confirmation available.
Show »
Length:894
Mass (Da):97,847
Checksum:i124043E01A5F8C85
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti319V → A in AAH60786 (PubMed:15489334).Curated1
Sequence conflicti521E → G in BAA91008 (PubMed:14702039).Curated1
Sequence conflicti596V → D in BAA91008 (PubMed:14702039).Curated1
Sequence conflicti802L → V in BAA91008 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_048295377E → A.Corresponds to variant rs16828486dbSNPEnsembl.1
Natural variantiVAR_035612617A → T in a colorectal cancer sample; somatic mutation. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0180131 – 496Missing in isoform 2. 1 PublicationAdd BLAST496
Alternative sequenceiVSP_039827142 – 158DSKKQ…YEAKM → ASLSLGSR in isoform 3. 1 PublicationAdd BLAST17

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB056749 mRNA. Translation: BAB85677.1.
AK000206 mRNA. Translation: BAA91008.1.
AK092291 mRNA. Translation: BAG52522.1.
AK295100 mRNA. Translation: BAG58136.1.
AL021154, AL357134 Genomic DNA. Translation: CAI19596.1.
AL357134, AL021154 Genomic DNA. Translation: CAI22177.1.
CH471134 Genomic DNA. Translation: EAW95050.1.
BC052305 mRNA. Translation: AAH52305.1.
BC060786 mRNA. Translation: AAH60786.1.
CCDSiCCDS235.1. [Q8TDY4-1]
CCDS44087.1. [Q8TDY4-3]
RefSeqiNP_001137250.1. NM_001143778.1. [Q8TDY4-3]
NP_060177.2. NM_017707.3. [Q8TDY4-1]
UniGeneiHs.437379.

Genome annotation databases

EnsembliENST00000336689; ENSP00000338769; ENSG00000088280. [Q8TDY4-1]
ENST00000437606; ENSP00000408826; ENSG00000088280. [Q8TDY4-3]
ENST00000495646; ENSP00000436150; ENSG00000088280. [Q8TDY4-2]
ENST00000634513; ENSP00000488934; ENSG00000282854. [Q8TDY4-2]
ENST00000634561; ENSP00000488946; ENSG00000282854. [Q8TDY4-1]
ENST00000635198; ENSP00000488953; ENSG00000282854. [Q8TDY4-3]
GeneIDi55616.
KEGGihsa:55616.
UCSCiuc001bgy.1. human. [Q8TDY4-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB056749 mRNA. Translation: BAB85677.1.
AK000206 mRNA. Translation: BAA91008.1.
AK092291 mRNA. Translation: BAG52522.1.
AK295100 mRNA. Translation: BAG58136.1.
AL021154, AL357134 Genomic DNA. Translation: CAI19596.1.
AL357134, AL021154 Genomic DNA. Translation: CAI22177.1.
CH471134 Genomic DNA. Translation: EAW95050.1.
BC052305 mRNA. Translation: AAH52305.1.
BC060786 mRNA. Translation: AAH60786.1.
CCDSiCCDS235.1. [Q8TDY4-1]
CCDS44087.1. [Q8TDY4-3]
RefSeqiNP_001137250.1. NM_001143778.1. [Q8TDY4-3]
NP_060177.2. NM_017707.3. [Q8TDY4-1]
UniGeneiHs.437379.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2B0OX-ray2.06E/F/G/H416-697[»]
3LVQX-ray3.38E416-697[»]
3LVRX-ray3.38E416-697[»]
ProteinModelPortaliQ8TDY4.
SMRiQ8TDY4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120756. 15 interactors.
IntActiQ8TDY4. 7 interactors.
STRINGi9606.ENSP00000338769.

PTM databases

iPTMnetiQ8TDY4.
PhosphoSitePlusiQ8TDY4.

Polymorphism and mutation databases

BioMutaiASAP3.
DMDMi74751433.

Proteomic databases

MaxQBiQ8TDY4.
PaxDbiQ8TDY4.
PeptideAtlasiQ8TDY4.
PRIDEiQ8TDY4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000336689; ENSP00000338769; ENSG00000088280. [Q8TDY4-1]
ENST00000437606; ENSP00000408826; ENSG00000088280. [Q8TDY4-3]
ENST00000495646; ENSP00000436150; ENSG00000088280. [Q8TDY4-2]
ENST00000634513; ENSP00000488934; ENSG00000282854. [Q8TDY4-2]
ENST00000634561; ENSP00000488946; ENSG00000282854. [Q8TDY4-1]
ENST00000635198; ENSP00000488953; ENSG00000282854. [Q8TDY4-3]
GeneIDi55616.
KEGGihsa:55616.
UCSCiuc001bgy.1. human. [Q8TDY4-1]

Organism-specific databases

CTDi55616.
DisGeNETi55616.
GeneCardsiASAP3.
H-InvDBHIX0000247.
HGNCiHGNC:14987. ASAP3.
HPAiHPA020537.
HPA020546.
MIMi616594. gene.
neXtProtiNX_Q8TDY4.
OpenTargetsiENSG00000088280.
ENSG00000282854.
PharmGKBiPA164716179.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0521. Eukaryota.
COG5347. LUCA.
GeneTreeiENSGT00760000118874.
HOGENOMiHOG000230570.
HOVERGENiHBG051327.
InParanoidiQ8TDY4.
KOiK12488.
OMAiIIQNGGH.
OrthoDBiEOG091G022H.
PhylomeDBiQ8TDY4.
TreeFamiTF325156.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000088280-MONOMER.

Miscellaneous databases

ChiTaRSiASAP3. human.
EvolutionaryTraceiQ8TDY4.
GenomeRNAii55616.
PROiQ8TDY4.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000088280.
CleanExiHS_ASAP3.
ExpressionAtlasiQ8TDY4. baseline and differential.
GenevisibleiQ8TDY4. HS.

Family and domain databases

Gene3Di1.20.1270.60. 1 hit.
1.25.40.20. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR027267. AH/BAR-dom.
IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001164. ArfGAP.
IPR028775. ASAP3.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PANTHERiPTHR23180:SF271. PTHR23180:SF271. 2 hits.
PfamiPF12796. Ank_2. 1 hit.
PF01412. ArfGap. 1 hit.
PF00169. PH. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00248. ANK. 2 hits.
SM00105. ArfGap. 1 hit.
SM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS50115. ARFGAP. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASAP3_HUMAN
AccessioniPrimary (citable) accession number: Q8TDY4
Secondary accession number(s): B3KRW0
, B4DHH4, Q6P9F4, Q86UY1, Q9NXK2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: June 1, 2002
Last modified: November 2, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.