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Protein

Serine/threonine-protein kinase Nek7

Gene

NEK7

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein kinase which plays an important role in mitotic cell cycle progression. Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis. Phosphorylates RPS6KB1.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Enzyme regulationi

Binding to NEK9 stimulates its activity by releasing the autoinhibitory function of Tyr-97.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei63ATP1
Sitei97Autoinhibitory1
Active sitei161Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi40 – 48ATP9

GO - Molecular functioni

GO - Biological processi

  • cytokinesis Source: UniProtKB
  • positive regulation of telomerase activity Source: BHF-UCL
  • positive regulation of telomere capping Source: BHF-UCL
  • positive regulation of telomere maintenance via telomerase Source: BHF-UCL
  • protein phosphorylation Source: UniProtKB
  • regulation of mitotic cell cycle Source: UniProtKB
  • spindle assembly Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:HS07733-MONOMER.
ReactomeiR-HSA-2980767. Activation of NIMA Kinases NEK9, NEK6, NEK7.
R-HSA-3301854. Nuclear Pore Complex (NPC) Disassembly.
SignaLinkiQ8TDX7.
SIGNORiQ8TDX7.

Protein family/group databases

TCDBi1.I.1.1.3. the nuclear pore complex (npc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase Nek7 (EC:2.7.11.1)
Alternative name(s):
Never in mitosis A-related kinase 7
Short name:
NimA-related protein kinase 7
Gene namesi
Name:NEK7
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:13386. NEK7.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi28Y → A: Significant decrease in catalytic activity; when associated with A-31. 1 Publication1
Mutagenesisi31L → A: Significant decrease in catalytic activity; when associated with A-28. 1 Publication1
Mutagenesisi33N → D: Significant decrease in catalytic activity. 1 Publication1
Mutagenesisi97Y → A: 5-fold increase in catalytic activity. 1 Publication1
Mutagenesisi97Y → F or L: Moderate increase in catalytic activity. 1 Publication1

Organism-specific databases

DisGeNETi140609.
OpenTargetsiENSG00000151414.
PharmGKBiPA31551.

Chemistry databases

ChEMBLiCHEMBL4849.
GuidetoPHARMACOLOGYi2122.

Polymorphism and mutation databases

BioMutaiNEK7.
DMDMi37537965.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000864301 – 302Serine/threonine-protein kinase Nek7Add BLAST302

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei5PhosphoserineCombined sources1
Modified residuei195Phosphoserine; by NEK91 Publication1

Post-translational modificationi

Phosphorylation at Ser-195 required for its activation.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8TDX7.
MaxQBiQ8TDX7.
PaxDbiQ8TDX7.
PeptideAtlasiQ8TDX7.
PRIDEiQ8TDX7.

PTM databases

iPTMnetiQ8TDX7.
PhosphoSitePlusiQ8TDX7.

Expressioni

Tissue specificityi

Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine.1 Publication

Gene expression databases

BgeeiENSG00000151414.
CleanExiHS_NEK7.
ExpressionAtlasiQ8TDX7. baseline and differential.
GenevisibleiQ8TDX7. HS.

Organism-specific databases

HPAiHPA015520.
HPA018193.

Interactioni

Subunit structurei

Monomer. Interacts with NEK9.2 Publications

Protein-protein interaction databases

BioGridi126636. 17 interactors.
DIPiDIP-50780N.
IntActiQ8TDX7. 12 interactors.
STRINGi9606.ENSP00000356355.

Chemistry databases

BindingDBiQ8TDX7.

Structurei

Secondary structure

1302
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi24 – 26Combined sources3
Helixi31 – 33Combined sources3
Beta strandi34 – 41Combined sources8
Beta strandi47 – 53Combined sources7
Turni54 – 56Combined sources3
Beta strandi59 – 65Combined sources7
Turni67 – 70Combined sources4
Helixi73 – 87Combined sources15
Beta strandi97 – 103Combined sources7
Beta strandi106 – 112Combined sources7
Helixi119 – 128Combined sources10
Helixi135 – 154Combined sources20
Helixi164 – 166Combined sources3
Beta strandi167 – 169Combined sources3
Beta strandi175 – 177Combined sources3
Helixi205 – 208Combined sources4
Helixi215 – 231Combined sources17
Helixi243 – 251Combined sources9
Turni260 – 262Combined sources3
Helixi265 – 274Combined sources10
Helixi279 – 281Combined sources3
Helixi285 – 299Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2WQMX-ray2.10A1-302[»]
2WQNX-ray2.30A1-302[»]
5DE2X-ray2.78A/B1-302[»]
ProteinModelPortaliQ8TDX7.
SMRiQ8TDX7.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8TDX7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini34 – 299Protein kinasePROSITE-ProRule annotationAdd BLAST266

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni20 – 33NTE motifAdd BLAST14

Domaini

Displays an autoinhibited conformation: Tyr-97 side chain points into the active site, interacts with the activation loop, and blocks the alphaC helix. The autoinhibitory conformation is released upon binding with NEK9.1 Publication
The NTE (N-terminal extension) motif is a structural component of the catalytic domain and thus contributes to activity.1 Publication

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0591. Eukaryota.
ENOG410XNQP. LUCA.
GeneTreeiENSGT00760000118997.
HOGENOMiHOG000233029.
HOVERGENiHBG105886.
InParanoidiQ8TDX7.
KOiK08857.
OMAiQLVNICI.
OrthoDBiEOG091G0AER.
PhylomeDBiQ8TDX7.
TreeFamiTF105135.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8TDX7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDEQSQGMQG PPVPQFQPQK ALRPDMGYNT LANFRIEKKI GRGQFSEVYR
60 70 80 90 100
AACLLDGVPV ALKKVQIFDL MDAKARADCI KEIDLLKQLN HPNVIKYYAS
110 120 130 140 150
FIEDNELNIV LELADAGDLS RMIKHFKKQK RLIPERTVWK YFVQLCSALE
160 170 180 190 200
HMHSRRVMHR DIKPANVFIT ATGVVKLGDL GLGRFFSSKT TAAHSLVGTP
210 220 230 240 250
YYMSPERIHE NGYNFKSDIW SLGCLLYEMA ALQSPFYGDK MNLYSLCKKI
260 270 280 290 300
EQCDYPPLPS DHYSEELRQL VNMCINPDPE KRPDVTYVYD VAKRMHACTA

SS
Length:302
Mass (Da):34,551
Last modified:June 1, 2002 - v1
Checksum:iC1FC990B34D94D4C
GO
Isoform 2 (identifier: Q8TDX7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     67-119: IFDLMDAKAR...VLELADAGDL → VCLLPVWERR...VSHHGSFAKK
     120-302: Missing.

Note: No experimental confirmation available.
Show »
Length:119
Mass (Da):13,402
Checksum:iC5CF3EAC5976AE9F
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04092435R → G.1 PublicationCorresponds to variant rs55833332dbSNPEnsembl.1
Natural variantiVAR_040925275I → M in an ovarian serous carcinoma sample; somatic mutation. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04124367 – 119IFDLM…DAGDL → VCLLPVWERRVCALNACYFL EIIKGSESLQYMATLTNLFE NLPVSHHGSFAKK in isoform 2. 1 PublicationAdd BLAST53
Alternative sequenceiVSP_041244120 – 302Missing in isoform 2. 1 PublicationAdd BLAST183

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB062450 mRNA. Translation: BAB85632.1.
CK819069 mRNA. No translation available.
AC093419 Genomic DNA. No translation available.
AC093561 Genomic DNA. No translation available.
CH471067 Genomic DNA. Translation: EAW91294.1.
CCDSiCCDS1394.1. [Q8TDX7-1]
RefSeqiNP_598001.1. NM_133494.2. [Q8TDX7-1]
UniGeneiHs.24119.
Hs.745083.

Genome annotation databases

EnsembliENST00000367383; ENSP00000356353; ENSG00000151414. [Q8TDX7-2]
ENST00000367385; ENSP00000356355; ENSG00000151414. [Q8TDX7-1]
ENST00000538004; ENSP00000444621; ENSG00000151414. [Q8TDX7-1]
GeneIDi140609.
KEGGihsa:140609.
UCSCiuc001gun.5. human. [Q8TDX7-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB062450 mRNA. Translation: BAB85632.1.
CK819069 mRNA. No translation available.
AC093419 Genomic DNA. No translation available.
AC093561 Genomic DNA. No translation available.
CH471067 Genomic DNA. Translation: EAW91294.1.
CCDSiCCDS1394.1. [Q8TDX7-1]
RefSeqiNP_598001.1. NM_133494.2. [Q8TDX7-1]
UniGeneiHs.24119.
Hs.745083.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2WQMX-ray2.10A1-302[»]
2WQNX-ray2.30A1-302[»]
5DE2X-ray2.78A/B1-302[»]
ProteinModelPortaliQ8TDX7.
SMRiQ8TDX7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi126636. 17 interactors.
DIPiDIP-50780N.
IntActiQ8TDX7. 12 interactors.
STRINGi9606.ENSP00000356355.

Chemistry databases

BindingDBiQ8TDX7.
ChEMBLiCHEMBL4849.
GuidetoPHARMACOLOGYi2122.

Protein family/group databases

TCDBi1.I.1.1.3. the nuclear pore complex (npc) family.

PTM databases

iPTMnetiQ8TDX7.
PhosphoSitePlusiQ8TDX7.

Polymorphism and mutation databases

BioMutaiNEK7.
DMDMi37537965.

Proteomic databases

EPDiQ8TDX7.
MaxQBiQ8TDX7.
PaxDbiQ8TDX7.
PeptideAtlasiQ8TDX7.
PRIDEiQ8TDX7.

Protocols and materials databases

DNASUi140609.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000367383; ENSP00000356353; ENSG00000151414. [Q8TDX7-2]
ENST00000367385; ENSP00000356355; ENSG00000151414. [Q8TDX7-1]
ENST00000538004; ENSP00000444621; ENSG00000151414. [Q8TDX7-1]
GeneIDi140609.
KEGGihsa:140609.
UCSCiuc001gun.5. human. [Q8TDX7-1]

Organism-specific databases

CTDi140609.
DisGeNETi140609.
GeneCardsiNEK7.
HGNCiHGNC:13386. NEK7.
HPAiHPA015520.
HPA018193.
MIMi606848. gene.
neXtProtiNX_Q8TDX7.
OpenTargetsiENSG00000151414.
PharmGKBiPA31551.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0591. Eukaryota.
ENOG410XNQP. LUCA.
GeneTreeiENSGT00760000118997.
HOGENOMiHOG000233029.
HOVERGENiHBG105886.
InParanoidiQ8TDX7.
KOiK08857.
OMAiQLVNICI.
OrthoDBiEOG091G0AER.
PhylomeDBiQ8TDX7.
TreeFamiTF105135.

Enzyme and pathway databases

BioCyciZFISH:HS07733-MONOMER.
ReactomeiR-HSA-2980767. Activation of NIMA Kinases NEK9, NEK6, NEK7.
R-HSA-3301854. Nuclear Pore Complex (NPC) Disassembly.
SignaLinkiQ8TDX7.
SIGNORiQ8TDX7.

Miscellaneous databases

ChiTaRSiNEK7. human.
EvolutionaryTraceiQ8TDX7.
GenomeRNAii140609.
PROiQ8TDX7.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000151414.
CleanExiHS_NEK7.
ExpressionAtlasiQ8TDX7. baseline and differential.
GenevisibleiQ8TDX7. HS.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNEK7_HUMAN
AccessioniPrimary (citable) accession number: Q8TDX7
Secondary accession number(s): A6NGT8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: June 1, 2002
Last modified: November 30, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.