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Q8TDU9 (RL3R2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Relaxin-3 receptor 2

Short name=RLN3 receptor 2
Alternative name(s):
G-protein coupled receptor 100
G-protein coupled receptor GPCR142
Insulin-like peptide INSL5 receptor
Relaxin family peptide receptor 4
Gene names
Name:RXFP4
Synonyms:GPR100, RLN3R2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length374 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

High affinity receptor for INSL5. Also acts as receptor for RLN3/relaxin-3, as well as bradykinin and kallidin. Binding of the ligand inhibit cAMP accumulation. Ref.1 Ref.9

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Expressed in a broader range of tissues including brain, kidney, testis, thymus, placenta, prostate, salivary gland, thyroid and colon. Ref.3

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Sequence caution

The sequence BAC05844.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   Coding sequence diversityPolymorphism
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Traceable author statement. Source: Reactome

   Molecular_functionangiotensin type II receptor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 374374Relaxin-3 receptor 2
PRO_0000070106

Regions

Topological domain1 – 4343Extracellular Potential
Transmembrane44 – 6421Helical; Name=1; Potential
Topological domain65 – 7814Cytoplasmic Potential
Transmembrane79 – 9921Helical; Name=2; Potential
Topological domain100 – 11617Extracellular Potential
Transmembrane117 – 13721Helical; Name=3; Potential
Topological domain138 – 15417Cytoplasmic Potential
Transmembrane155 – 17521Helical; Name=4; Potential
Topological domain176 – 20934Extracellular Potential
Transmembrane210 – 23021Helical; Name=5; Potential
Topological domain231 – 24919Cytoplasmic Potential
Transmembrane250 – 27021Helical; Name=6; Potential
Topological domain271 – 28111Extracellular Potential
Transmembrane282 – 30221Helical; Name=7; Potential
Topological domain303 – 37472Cytoplasmic Potential

Amino acid modifications

Glycosylation51N-linked (GlcNAc...) Potential
Glycosylation171N-linked (GlcNAc...) Potential
Disulfide bond114 ↔ 191 By similarity

Natural variations

Natural variant3291L → S. Ref.1 Ref.7 Ref.8
Corresponds to variant rs2152051 [ dbSNP | Ensembl ].
VAR_021516

Sequences

Sequence LengthMass (Da)Tools
Q8TDU9 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 1929EA812C0804DA

FASTA37441,141
        10         20         30         40         50         60 
MPTLNTSASP PTFFWANASG GSVLSADDAP MPVKFLALRL MVALAYGLVG AIGLLGNLAV 

        70         80         90        100        110        120 
LWVLSNCARR APGPPSDTFV FNLALADLGL ALTLPFWAAE SALDFHWPFG GALCKMVLTA 

       130        140        150        160        170        180 
TVLNVYASIF LITALSVARY WVVAMAAGPG THLSLFWARI ATLAVWAAAA LVTVPTAVFG 

       190        200        210        220        230        240 
VEGEVCGVRL CLLRFPSRYW LGAYQLQRVV LAFMVPLGVI TTSYLLLLAF LQRRQRRRQD 

       250        260        270        280        290        300 
SRVVARSVRI LVASFFLCWF PNHVVTLWGV LVKFDLVPWN STFYTIQTYV FPVTTCLAHS 

       310        320        330        340        350        360 
NSCLNPVLYC LLRREPRQAL AGTFRDLRLR LWPQGGGWVQ QVALKQVGRR WVASNPRESR 

       370 
PSTLLTNLDR GTPG 

« Hide

References

« Hide 'large scale' references
[1]"Identification of relaxin-3/INSL7 as a ligand for GPCR142."
Liu C., Chen J., Sutton S., Roland B., Kuei C., Farmer N., Sillard R., Lovenberg T.W.
J. Biol. Chem. 278:50765-50770(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT SER-329, FUNCTION AS A RECEPTOR FOR RLN3.
[2]"Seven evolutionarily conserved human rhodopsin G protein-coupled receptors lacking close relatives."
Fredriksson R., Hoeglund P.J., Gloriam D.E.I., Lagerstroem M.C., Schioeth H.B.
FEBS Lett. 554:381-388(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Identification and characterisation of GPR100 as a novel human G-protein-coupled bradykinin receptor."
Boels K., Schaller H.C.
Br. J. Pharmacol. 140:932-938(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INTERACTION WITH BRADYKININ AND KALLIDIN.
Tissue: Pancreatic carcinoma.
[4]"Genome-wide discovery and analysis of human seven transmembrane helix receptor genes."
Suwa M., Sato T., Okouchi I., Arita M., Futami K., Matsumoto S., Tsutsumi S., Aburatani H., Asai K., Akiyama Y.
Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"Identification of G protein-coupled receptor genes from the human genome sequence."
Takeda S., Kadowaki S., Haga T., Takaesu H., Mitaku S.
FEBS Lett. 520:97-101(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]Kaighin V.A., Martin A.L., Aronstam R.S.
Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT SER-329.
Tissue: Testis.
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT SER-329.
Tissue: Brain.
[9]"INSL5 is a high affinity specific agonist for GPCR142 (GPR100)."
Liu C., Kuei C., Sutton S., Chen J., Bonaventure P., Wu J., Nepomuceno D., Kamme F., Tran D.T., Zhu J., Wilkinson T., Bathgate R., Eriste E., Sillard R., Lovenberg T.W.
J. Biol. Chem. 280:292-300(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS A RECEPTOR FOR INSL5.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY394502 mRNA. Translation: AAQ92316.1.
AY288415 mRNA. Translation: AAP72124.1.
AY170824 mRNA. Translation: AAO17676.1.
AB065617 Genomic DNA. Translation: BAC05844.1. Sequence problems.
AB083593 Genomic DNA. Translation: BAB89306.1.
AL355388 Genomic DNA. Translation: CAH72626.1.
EU432129 mRNA. Translation: ABY87928.1.
BC101507 mRNA. Translation: AAI01508.1.
BC101509 mRNA. Translation: AAI01510.1.
RefSeqNP_871001.1. NM_181885.2.
UniGeneHs.449914.

3D structure databases

ProteinModelPortalQ8TDU9.
SMRQ8TDU9. Positions 36-323.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid130880. 1 interaction.
STRING9606.ENSP00000357301.

Chemistry

ChEMBLCHEMBL1628473.
GuidetoPHARMACOLOGY354.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteQ8TDU9.

Polymorphism databases

DMDM38258194.

Proteomic databases

PaxDbQ8TDU9.
PRIDEQ8TDU9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000368318; ENSP00000357301; ENSG00000173080.
GeneID339403.
KEGGhsa:339403.
UCSCuc010pgs.2. human.

Organism-specific databases

CTD339403.
GeneCardsGC01P155911.
HGNCHGNC:14666. RXFP4.
MIM609043. gene.
neXtProtNX_Q8TDU9.
PharmGKBPA28848.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG292372.
HOGENOMHOG000230485.
HOVERGENHBG101327.
InParanoidQ8TDU9.
KOK08398.
OMACLLRFPS.
OrthoDBEOG7ZD1VG.
PhylomeDBQ8TDU9.
TreeFamTF330024.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

BgeeQ8TDU9.
CleanExHS_RXFP4.
GenevestigatorQ8TDU9.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR000248. ATII_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00241. ANGIOTENSINR.
PR00237. GPCRRHODOPSN.
PROSITEPS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiRelaxin/insulin-like_family_peptide_receptor_4.
GenomeRNAi339403.
NextBio97386.
PROQ8TDU9.
SOURCESearch...

Entry information

Entry nameRL3R2_HUMAN
AccessionPrimary (citable) accession number: Q8TDU9
Secondary accession number(s): B0M0L4, Q3MJB1, Q8NGZ8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 7, 2003
Last sequence update: June 1, 2002
Last modified: April 16, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries