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Protein

Relaxin-3 receptor 2

Gene

RXFP4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

High affinity receptor for INSL5. Also acts as receptor for RLN3/relaxin-3, as well as bradykinin and kallidin. Binding of the ligand inhibit cAMP accumulation.2 Publications

GO - Molecular functioni

  1. angiotensin type II receptor activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_19231. G alpha (i) signalling events.
REACT_21314. Relaxin receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Relaxin-3 receptor 2
Short name:
RLN3 receptor 2
Alternative name(s):
G-protein coupled receptor 100
G-protein coupled receptor GPCR142
Insulin-like peptide INSL5 receptor
Relaxin family peptide receptor 4
Gene namesi
Name:RXFP4
Synonyms:GPR100, RLN3R2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:14666. RXFP4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4343ExtracellularSequence AnalysisAdd
BLAST
Transmembranei44 – 6421Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini65 – 7814CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei79 – 9921Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini100 – 11617ExtracellularSequence AnalysisAdd
BLAST
Transmembranei117 – 13721Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini138 – 15417CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei155 – 17521Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini176 – 20934ExtracellularSequence AnalysisAdd
BLAST
Transmembranei210 – 23021Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini231 – 24919CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei250 – 27021Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini271 – 28111ExtracellularSequence AnalysisAdd
BLAST
Transmembranei282 – 30221Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini303 – 37472CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA28848.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 374374Relaxin-3 receptor 2PRO_0000070106Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi5 – 51N-linked (GlcNAc...)Sequence Analysis
Glycosylationi17 – 171N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi114 ↔ 191PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8TDU9.
PRIDEiQ8TDU9.

PTM databases

PhosphoSiteiQ8TDU9.

Expressioni

Tissue specificityi

Expressed in a broader range of tissues including brain, kidney, testis, thymus, placenta, prostate, salivary gland, thyroid and colon.1 Publication

Gene expression databases

BgeeiQ8TDU9.
CleanExiHS_RXFP4.
GenevestigatoriQ8TDU9.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
RLN3Q8WXF39EBI-9519524,EBI-9519546

Protein-protein interaction databases

BioGridi130880. 1 interaction.
IntActiQ8TDU9. 1 interaction.
STRINGi9606.ENSP00000357301.

Structurei

3D structure databases

ProteinModelPortaliQ8TDU9.
SMRiQ8TDU9. Positions 36-312.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG292372.
GeneTreeiENSGT00760000119055.
HOGENOMiHOG000230485.
HOVERGENiHBG101327.
InParanoidiQ8TDU9.
KOiK08398.
OMAiCLLRFPS.
OrthoDBiEOG7ZD1VG.
PhylomeDBiQ8TDU9.
TreeFamiTF330024.

Family and domain databases

InterProiIPR000248. ATII_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00241. ANGIOTENSINR.
PR00237. GPCRRHODOPSN.
PROSITEiPS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8TDU9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTLNTSASP PTFFWANASG GSVLSADDAP MPVKFLALRL MVALAYGLVG
60 70 80 90 100
AIGLLGNLAV LWVLSNCARR APGPPSDTFV FNLALADLGL ALTLPFWAAE
110 120 130 140 150
SALDFHWPFG GALCKMVLTA TVLNVYASIF LITALSVARY WVVAMAAGPG
160 170 180 190 200
THLSLFWARI ATLAVWAAAA LVTVPTAVFG VEGEVCGVRL CLLRFPSRYW
210 220 230 240 250
LGAYQLQRVV LAFMVPLGVI TTSYLLLLAF LQRRQRRRQD SRVVARSVRI
260 270 280 290 300
LVASFFLCWF PNHVVTLWGV LVKFDLVPWN STFYTIQTYV FPVTTCLAHS
310 320 330 340 350
NSCLNPVLYC LLRREPRQAL AGTFRDLRLR LWPQGGGWVQ QVALKQVGRR
360 370
WVASNPRESR PSTLLTNLDR GTPG
Length:374
Mass (Da):41,141
Last modified:May 31, 2002 - v1
Checksum:i1929EA812C0804DA
GO

Sequence cautioni

The sequence BAC05844.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti329 – 3291L → S.3 Publications
Corresponds to variant rs2152051 [ dbSNP | Ensembl ].
VAR_021516

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY394502 mRNA. Translation: AAQ92316.1.
AY288415 mRNA. Translation: AAP72124.1.
AY170824 mRNA. Translation: AAO17676.1.
AB065617 Genomic DNA. Translation: BAC05844.1. Sequence problems.
AB083593 Genomic DNA. Translation: BAB89306.1.
AL355388 Genomic DNA. Translation: CAH72626.1.
EU432129 mRNA. Translation: ABY87928.1.
BC101507 mRNA. Translation: AAI01508.1.
BC101509 mRNA. Translation: AAI01510.1.
CCDSiCCDS1124.1.
RefSeqiNP_871001.1. NM_181885.2.
UniGeneiHs.449914.

Genome annotation databases

EnsembliENST00000368318; ENSP00000357301; ENSG00000173080.
GeneIDi339403.
KEGGihsa:339403.
UCSCiuc010pgs.2. human.

Polymorphism databases

DMDMi38258194.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY394502 mRNA. Translation: AAQ92316.1.
AY288415 mRNA. Translation: AAP72124.1.
AY170824 mRNA. Translation: AAO17676.1.
AB065617 Genomic DNA. Translation: BAC05844.1. Sequence problems.
AB083593 Genomic DNA. Translation: BAB89306.1.
AL355388 Genomic DNA. Translation: CAH72626.1.
EU432129 mRNA. Translation: ABY87928.1.
BC101507 mRNA. Translation: AAI01508.1.
BC101509 mRNA. Translation: AAI01510.1.
CCDSiCCDS1124.1.
RefSeqiNP_871001.1. NM_181885.2.
UniGeneiHs.449914.

3D structure databases

ProteinModelPortaliQ8TDU9.
SMRiQ8TDU9. Positions 36-312.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi130880. 1 interaction.
IntActiQ8TDU9. 1 interaction.
STRINGi9606.ENSP00000357301.

Chemistry

BindingDBiQ8TDU9.
ChEMBLiCHEMBL1628473.
GuidetoPHARMACOLOGYi354.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiQ8TDU9.

Polymorphism databases

DMDMi38258194.

Proteomic databases

PaxDbiQ8TDU9.
PRIDEiQ8TDU9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000368318; ENSP00000357301; ENSG00000173080.
GeneIDi339403.
KEGGihsa:339403.
UCSCiuc010pgs.2. human.

Organism-specific databases

CTDi339403.
GeneCardsiGC01P155911.
HGNCiHGNC:14666. RXFP4.
MIMi609043. gene.
neXtProtiNX_Q8TDU9.
PharmGKBiPA28848.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG292372.
GeneTreeiENSGT00760000119055.
HOGENOMiHOG000230485.
HOVERGENiHBG101327.
InParanoidiQ8TDU9.
KOiK08398.
OMAiCLLRFPS.
OrthoDBiEOG7ZD1VG.
PhylomeDBiQ8TDU9.
TreeFamiTF330024.

Enzyme and pathway databases

ReactomeiREACT_19231. G alpha (i) signalling events.
REACT_21314. Relaxin receptors.

Miscellaneous databases

GeneWikiiRelaxin/insulin-like_family_peptide_receptor_4.
GenomeRNAii339403.
NextBioi97386.
PROiQ8TDU9.
SOURCEiSearch...

Gene expression databases

BgeeiQ8TDU9.
CleanExiHS_RXFP4.
GenevestigatoriQ8TDU9.

Family and domain databases

InterProiIPR000248. ATII_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00241. ANGIOTENSINR.
PR00237. GPCRRHODOPSN.
PROSITEiPS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT SER-329, FUNCTION AS A RECEPTOR FOR RLN3.
  2. "Seven evolutionarily conserved human rhodopsin G protein-coupled receptors lacking close relatives."
    Fredriksson R., Hoeglund P.J., Gloriam D.E.I., Lagerstroem M.C., Schioeth H.B.
    FEBS Lett. 554:381-388(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Identification and characterisation of GPR100 as a novel human G-protein-coupled bradykinin receptor."
    Boels K., Schaller H.C.
    Br. J. Pharmacol. 140:932-938(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INTERACTION WITH BRADYKININ AND KALLIDIN.
    Tissue: Pancreatic carcinoma.
  4. "Genome-wide discovery and analysis of human seven transmembrane helix receptor genes."
    Suwa M., Sato T., Okouchi I., Arita M., Futami K., Matsumoto S., Tsutsumi S., Aburatani H., Asai K., Akiyama Y.
    Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. "Identification of G protein-coupled receptor genes from the human genome sequence."
    Takeda S., Kadowaki S., Haga T., Takaesu H., Mitaku S.
    FEBS Lett. 520:97-101(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. Kaighin V.A., Martin A.L., Aronstam R.S.
    Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT SER-329.
    Tissue: Testis.
  8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT SER-329.
    Tissue: Brain.
  9. Cited for: FUNCTION AS A RECEPTOR FOR INSL5.

Entry informationi

Entry nameiRL3R2_HUMAN
AccessioniPrimary (citable) accession number: Q8TDU9
Secondary accession number(s): B0M0L4, Q3MJB1, Q8NGZ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 6, 2003
Last sequence update: May 31, 2002
Last modified: January 6, 2015
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.