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Q8TDR2

- STK35_HUMAN

UniProt

Q8TDR2 - STK35_HUMAN

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Protein

Serine/threonine-protein kinase 35

Gene

STK35

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei231 – 2311ATPCurated
Active sitei360 – 3601Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi208 – 2169ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protein serine/threonine kinase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

SignaLinkiQ8TDR2.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase 35 (EC:2.7.11.1)
Alternative name(s):
CLP-36-interacting kinase 1
Short name:
CLIK-1
PDLIM1-interacting kinase 1
Serine/threonine-protein kinase 35 L1
Gene namesi
Name:STK35
Synonyms:CLIK1, PDIK1, STK35L1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 20

Organism-specific databases

HGNCiHGNC:16254. STK35.

Subcellular locationi

Nucleus. Nucleusnucleolus. Cytoplasm
Note: When associated with PDLIM1, it is mostly found in cytoplasm, localized to actin stress fibers (PubMed:11973348). However, PubMed:19756140 detected STK35 only in the nucleus, and the presence of PDLIM1 had no influence on its location.1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi231 – 2311K → M: No autophosphorylation. 1 Publication

Organism-specific databases

PharmGKBiPA38097.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 534534Serine/threonine-protein kinase 35PRO_0000086717Add
BLAST

Post-translational modificationi

Autophosphorylated.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8TDR2.
PaxDbiQ8TDR2.
PRIDEiQ8TDR2.

PTM databases

PhosphoSiteiQ8TDR2.

Expressioni

Tissue specificityi

Expressed in testis.

Gene expression databases

BgeeiQ8TDR2.
CleanExiHS_STK35.
GenevestigatoriQ8TDR2.

Organism-specific databases

HPAiHPA051673.

Interactioni

Subunit structurei

Interacts with PDLIM1/CLP-36.

Protein-protein interaction databases

BioGridi126760. 3 interactions.
STRINGi9606.ENSP00000246032.

Structurei

3D structure databases

ProteinModelPortaliQ8TDR2.
SMRiQ8TDR2. Positions 201-521.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini202 – 530329Protein kinasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi40 – 187148Ala-richAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00770000120504.
HOVERGENiHBG056829.
InParanoidiQ8TDR2.
KOiK08859.
OMAiPRGKEGH.
OrthoDBiEOG76DTSN.
PhylomeDBiQ8TDR2.
TreeFamiTF105336.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8TDR2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGHQESPLAR APAGGAAYVK RLCKGLSWRE HVESHGSLGA QASPASAAAA
60 70 80 90 100
EGSATRRARA ATSRAARSRR QPGPGADHPQ AGAPGGKRAA RKWRCAGQVT
110 120 130 140 150
IQGPAPPRPR AGRRDEAGGA RAAPLLLPPP PAAMETGKDG ARRGTQSPER
160 170 180 190 200
KRRSPVPRAP STKLRPAAAA RAMDPVAAEA PGEAFLARRR PEGGGGSARP
210 220 230 240 250
RYSLLAEIGR GSYGVVYEAV AGRSGARVAV KKIRCDAPEN VELALAEFWA
260 270 280 290 300
LTSLKRRHQN VVQFEECVLQ RNGLAQRMSH GNKSSQLYLR LVETSLKGER
310 320 330 340 350
ILGYAEEPCY LWFVMEFCEG GDLNQYVLSR RPDPATNKSF MLQLTSAIAF
360 370 380 390 400
LHKNHIVHRD LKPDNILITE RSGTPILKVA DFGLSKVCAG LAPRGKEGNQ
410 420 430 440 450
DNKNVNVNKY WLSSACGSDF YMAPEVWEGH YTAKADIFAL GIIIWAMIER
460 470 480 490 500
ITFIDSETKK ELLGTYIKQG TEIVPVGEAL LENPKMELHI PQKRRTSMSE
510 520 530
GIKQLLKDML AANPQDRPDA FELETRMDQV TCAA
Length:534
Mass (Da):58,051
Last modified:March 23, 2010 - v2
Checksum:iCA1A99D26829C9AF
GO

Sequence cautioni

The sequence AAI40884.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAI40885.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAG37270.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAI15590.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti69 – 691R → G in BAG37270. (PubMed:14702039)Curated
Sequence conflicti115 – 1151D → G in BAG37270. (PubMed:14702039)Curated
Sequence conflicti288 – 30720YLRLV…GYAEE → GNGEGRRPQRYTKPGAEKAK in AAI11574. (PubMed:15489334)CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GQ281297 mRNA. Translation: ACU33925.1.
AL359916 Genomic DNA. Translation: CAI15590.1. Sequence problems.
AK314728 mRNA. Translation: BAG37270.1. Different initiation.
BC017340 mRNA. Translation: AAH17340.2.
BC111573 mRNA. Translation: AAI11574.1.
BC140883 mRNA. Translation: AAI40884.1. Different initiation.
BC140884 mRNA. Translation: AAI40885.1. Different initiation.
AF195026 mRNA. Translation: AAL99353.1.
CCDSiCCDS13024.2.
RefSeqiNP_543026.2. NM_080836.3.
UniGeneiHs.100057.

Genome annotation databases

EnsembliENST00000381482; ENSP00000370891; ENSG00000125834.
ENST00000493263; ENSP00000426612; ENSG00000125834.
GeneIDi140901.
KEGGihsa:140901.
UCSCiuc002wfw.4. human.

Polymorphism databases

DMDMi292495039.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GQ281297 mRNA. Translation: ACU33925.1 .
AL359916 Genomic DNA. Translation: CAI15590.1 . Sequence problems.
AK314728 mRNA. Translation: BAG37270.1 . Different initiation.
BC017340 mRNA. Translation: AAH17340.2 .
BC111573 mRNA. Translation: AAI11574.1 .
BC140883 mRNA. Translation: AAI40884.1 . Different initiation.
BC140884 mRNA. Translation: AAI40885.1 . Different initiation.
AF195026 mRNA. Translation: AAL99353.1 .
CCDSi CCDS13024.2.
RefSeqi NP_543026.2. NM_080836.3.
UniGenei Hs.100057.

3D structure databases

ProteinModelPortali Q8TDR2.
SMRi Q8TDR2. Positions 201-521.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 126760. 3 interactions.
STRINGi 9606.ENSP00000246032.

Chemistry

BindingDBi Q8TDR2.
ChEMBLi CHEMBL5651.
GuidetoPHARMACOLOGYi 2222.

PTM databases

PhosphoSitei Q8TDR2.

Polymorphism databases

DMDMi 292495039.

Proteomic databases

MaxQBi Q8TDR2.
PaxDbi Q8TDR2.
PRIDEi Q8TDR2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000381482 ; ENSP00000370891 ; ENSG00000125834 .
ENST00000493263 ; ENSP00000426612 ; ENSG00000125834 .
GeneIDi 140901.
KEGGi hsa:140901.
UCSCi uc002wfw.4. human.

Organism-specific databases

CTDi 140901.
GeneCardsi GC20P002082.
H-InvDB HIX0040513.
HIX0040701.
HGNCi HGNC:16254. STK35.
HPAi HPA051673.
MIMi 609370. gene.
neXtProti NX_Q8TDR2.
PharmGKBi PA38097.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00770000120504.
HOVERGENi HBG056829.
InParanoidi Q8TDR2.
KOi K08859.
OMAi PRGKEGH.
OrthoDBi EOG76DTSN.
PhylomeDBi Q8TDR2.
TreeFami TF105336.

Enzyme and pathway databases

SignaLinki Q8TDR2.

Miscellaneous databases

GeneWikii Serine/threonine_kinase_35.
STK35L1.
GenomeRNAii 140901.
NextBioi 84567.
PROi Q8TDR2.
SOURCEi Search...

Gene expression databases

Bgeei Q8TDR2.
CleanExi HS_STK35.
Genevestigatori Q8TDR2.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 2 hits.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identifying and characterizing a novel protein kinase STK35L1 and deciphering its orthologs and close-homologs in vertebrates."
    Goyal P., Behring A., Kumar A., Siess W.
    PLoS ONE 4:E6981-E6981(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
  2. "The DNA sequence and comparative analysis of human chromosome 20."
    Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E.
    , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
    Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 51-534.
    Tissue: Cerebellum.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 101-534.
    Tissue: Skin.
  5. "Clik1: a novel kinase targeted to actin stress fibers by the CLP-36 PDZ-LIM protein."
    Vallenius T., Maekelae T.P.
    J. Cell Sci. 115:2067-2073(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 134-534, MUTAGENESIS OF LYS-231, SUBCELLULAR LOCATION, AUTOPHOSPHORYLATION.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSTK35_HUMAN
AccessioniPrimary (citable) accession number: Q8TDR2
Secondary accession number(s): B2RBM3
, C7ENV8, Q2NKW6, Q5T3R1, Q5T3R2, Q96AB4, Q9BZ06
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 27, 2003
Last sequence update: March 23, 2010
Last modified: November 26, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Association with PDLIM1 is controversial.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3