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Q8TDR2

- STK35_HUMAN

UniProt

Q8TDR2 - STK35_HUMAN

Protein

Serine/threonine-protein kinase 35

Gene

STK35

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 2 (23 Mar 2010)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei231 – 2311ATPCurated
    Active sitei360 – 3601Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi208 – 2169ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein serine/threonine kinase activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    SignaLinkiQ8TDR2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein kinase 35 (EC:2.7.11.1)
    Alternative name(s):
    CLP-36-interacting kinase 1
    Short name:
    CLIK-1
    PDLIM1-interacting kinase 1
    Serine/threonine-protein kinase 35 L1
    Gene namesi
    Name:STK35
    Synonyms:CLIK1, PDIK1, STK35L1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 20

    Organism-specific databases

    HGNCiHGNC:16254. STK35.

    Subcellular locationi

    Nucleus. Nucleusnucleolus. Cytoplasm
    Note: When associated with PDLIM1, it is mostly found in cytoplasm, localized to actin stress fibers (PubMed:11973348). However, PubMed:19756140 detected STK35 only in the nucleus, and the presence of PDLIM1 had no influence on its location.1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleolus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi231 – 2311K → M: No autophosphorylation. 1 Publication

    Organism-specific databases

    PharmGKBiPA38097.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 534534Serine/threonine-protein kinase 35PRO_0000086717Add
    BLAST

    Post-translational modificationi

    Autophosphorylated.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ8TDR2.
    PaxDbiQ8TDR2.
    PRIDEiQ8TDR2.

    PTM databases

    PhosphoSiteiQ8TDR2.

    Expressioni

    Tissue specificityi

    Expressed in testis.

    Gene expression databases

    ArrayExpressiQ8TDR2.
    BgeeiQ8TDR2.
    CleanExiHS_STK35.
    GenevestigatoriQ8TDR2.

    Organism-specific databases

    HPAiHPA051673.

    Interactioni

    Subunit structurei

    Interacts with PDLIM1/CLP-36.

    Protein-protein interaction databases

    BioGridi126760. 3 interactions.
    STRINGi9606.ENSP00000246032.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8TDR2.
    SMRiQ8TDR2. Positions 201-521.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini202 – 530329Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi40 – 187148Ala-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOVERGENiHBG056829.
    InParanoidiQ8TDR2.
    KOiK08859.
    OMAiPRGKEGH.
    OrthoDBiEOG76DTSN.
    PhylomeDBiQ8TDR2.
    TreeFamiTF105336.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 2 hits.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8TDR2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGHQESPLAR APAGGAAYVK RLCKGLSWRE HVESHGSLGA QASPASAAAA    50
    EGSATRRARA ATSRAARSRR QPGPGADHPQ AGAPGGKRAA RKWRCAGQVT 100
    IQGPAPPRPR AGRRDEAGGA RAAPLLLPPP PAAMETGKDG ARRGTQSPER 150
    KRRSPVPRAP STKLRPAAAA RAMDPVAAEA PGEAFLARRR PEGGGGSARP 200
    RYSLLAEIGR GSYGVVYEAV AGRSGARVAV KKIRCDAPEN VELALAEFWA 250
    LTSLKRRHQN VVQFEECVLQ RNGLAQRMSH GNKSSQLYLR LVETSLKGER 300
    ILGYAEEPCY LWFVMEFCEG GDLNQYVLSR RPDPATNKSF MLQLTSAIAF 350
    LHKNHIVHRD LKPDNILITE RSGTPILKVA DFGLSKVCAG LAPRGKEGNQ 400
    DNKNVNVNKY WLSSACGSDF YMAPEVWEGH YTAKADIFAL GIIIWAMIER 450
    ITFIDSETKK ELLGTYIKQG TEIVPVGEAL LENPKMELHI PQKRRTSMSE 500
    GIKQLLKDML AANPQDRPDA FELETRMDQV TCAA 534
    Length:534
    Mass (Da):58,051
    Last modified:March 23, 2010 - v2
    Checksum:iCA1A99D26829C9AF
    GO

    Sequence cautioni

    The sequence AAI40884.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
    The sequence AAI40885.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
    The sequence BAG37270.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
    The sequence CAI15590.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti69 – 691R → G in BAG37270. (PubMed:14702039)Curated
    Sequence conflicti115 – 1151D → G in BAG37270. (PubMed:14702039)Curated
    Sequence conflicti288 – 30720YLRLV…GYAEE → GNGEGRRPQRYTKPGAEKAK in AAI11574. (PubMed:15489334)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    GQ281297 mRNA. Translation: ACU33925.1.
    AL359916 Genomic DNA. Translation: CAI15590.1. Sequence problems.
    AK314728 mRNA. Translation: BAG37270.1. Different initiation.
    BC017340 mRNA. Translation: AAH17340.2.
    BC111573 mRNA. Translation: AAI11574.1.
    BC140883 mRNA. Translation: AAI40884.1. Different initiation.
    BC140884 mRNA. Translation: AAI40885.1. Different initiation.
    AF195026 mRNA. Translation: AAL99353.1.
    CCDSiCCDS13024.2.
    RefSeqiNP_543026.2. NM_080836.3.
    UniGeneiHs.100057.

    Genome annotation databases

    EnsembliENST00000381482; ENSP00000370891; ENSG00000125834.
    ENST00000493263; ENSP00000426612; ENSG00000125834.
    GeneIDi140901.
    KEGGihsa:140901.
    UCSCiuc002wfw.4. human.

    Polymorphism databases

    DMDMi292495039.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    GQ281297 mRNA. Translation: ACU33925.1 .
    AL359916 Genomic DNA. Translation: CAI15590.1 . Sequence problems.
    AK314728 mRNA. Translation: BAG37270.1 . Different initiation.
    BC017340 mRNA. Translation: AAH17340.2 .
    BC111573 mRNA. Translation: AAI11574.1 .
    BC140883 mRNA. Translation: AAI40884.1 . Different initiation.
    BC140884 mRNA. Translation: AAI40885.1 . Different initiation.
    AF195026 mRNA. Translation: AAL99353.1 .
    CCDSi CCDS13024.2.
    RefSeqi NP_543026.2. NM_080836.3.
    UniGenei Hs.100057.

    3D structure databases

    ProteinModelPortali Q8TDR2.
    SMRi Q8TDR2. Positions 201-521.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 126760. 3 interactions.
    STRINGi 9606.ENSP00000246032.

    Chemistry

    BindingDBi Q8TDR2.
    ChEMBLi CHEMBL5651.
    GuidetoPHARMACOLOGYi 2222.

    PTM databases

    PhosphoSitei Q8TDR2.

    Polymorphism databases

    DMDMi 292495039.

    Proteomic databases

    MaxQBi Q8TDR2.
    PaxDbi Q8TDR2.
    PRIDEi Q8TDR2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000381482 ; ENSP00000370891 ; ENSG00000125834 .
    ENST00000493263 ; ENSP00000426612 ; ENSG00000125834 .
    GeneIDi 140901.
    KEGGi hsa:140901.
    UCSCi uc002wfw.4. human.

    Organism-specific databases

    CTDi 140901.
    GeneCardsi GC20P002082.
    H-InvDB HIX0040513.
    HIX0040701.
    HGNCi HGNC:16254. STK35.
    HPAi HPA051673.
    MIMi 609370. gene.
    neXtProti NX_Q8TDR2.
    PharmGKBi PA38097.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0515.
    HOVERGENi HBG056829.
    InParanoidi Q8TDR2.
    KOi K08859.
    OMAi PRGKEGH.
    OrthoDBi EOG76DTSN.
    PhylomeDBi Q8TDR2.
    TreeFami TF105336.

    Enzyme and pathway databases

    SignaLinki Q8TDR2.

    Miscellaneous databases

    GeneWikii Serine/threonine_kinase_35.
    STK35L1.
    GenomeRNAii 140901.
    NextBioi 84567.
    PROi Q8TDR2.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8TDR2.
    Bgeei Q8TDR2.
    CleanExi HS_STK35.
    Genevestigatori Q8TDR2.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 2 hits.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identifying and characterizing a novel protein kinase STK35L1 and deciphering its orthologs and close-homologs in vertebrates."
      Goyal P., Behring A., Kumar A., Siess W.
      PLoS ONE 4:E6981-E6981(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
    2. "The DNA sequence and comparative analysis of human chromosome 20."
      Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E.
      , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
      Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 51-534.
      Tissue: Cerebellum.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 101-534.
      Tissue: Skin.
    5. "Clik1: a novel kinase targeted to actin stress fibers by the CLP-36 PDZ-LIM protein."
      Vallenius T., Maekelae T.P.
      J. Cell Sci. 115:2067-2073(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 134-534, MUTAGENESIS OF LYS-231, SUBCELLULAR LOCATION, AUTOPHOSPHORYLATION.
    6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSTK35_HUMAN
    AccessioniPrimary (citable) accession number: Q8TDR2
    Secondary accession number(s): B2RBM3
    , C7ENV8, Q2NKW6, Q5T3R1, Q5T3R2, Q96AB4, Q9BZ06
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 27, 2003
    Last sequence update: March 23, 2010
    Last modified: October 1, 2014
    This is version 121 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    Association with PDLIM1 is controversial.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 20
      Human chromosome 20: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3