Q8TDR2 (STK35_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase 35
EC=2.7.11.1

Alternative name(s):
CLP-36-interacting kinase 1
Short name=CLIK-1
PDLIM1-interacting kinase 1
Serine/threonine-protein kinase 35 L1

Gene names

Name:	STK35
Synonyms:	CLIK1, PDIK1, STK35L1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	534 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Interacts with PDLIM1/CLP-36.
Subcellular location	Nucleus. Nucleus › nucleolus. Cytoplasm. Note: When associated with PDLIM1, it is mostly found in cytoplasm, localized to actin stress fibers (Ref.5). However, Ref.1 detected STK35 only in the nucleus, and the presence of PDLIM1 had no influence on its location. Ref.1 Ref.5
Tissue specificity	Expressed in testis.
Post-translational modification	Autophosphorylated. Ref.5
Miscellaneous	Association with PDLIM1 is controversial (Ref.5).
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.
Sequence caution	The sequence AAI40884.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAI40885.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAG37270.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence CAI15590.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 534	534	Serine/threonine-protein kinase 35		PRO_0000086717
Regions
	Domain	202 – 530	329	Protein kinase
	Nucleotide binding	208 – 216	9	ATP By similarity
	Compositional bias	40 – 187	148	Ala-rich
Sites
	Active site	360	1	Proton acceptor By similarity
	Binding site	231	1	ATP Probable
Experimental info
	Mutagenesis	231	1	K → M: No autophosphorylation. Ref.5
	Sequence conflict	69	1	R → G in BAG37270. Ref.3
	Sequence conflict	115	1	D → G in BAG37270. Ref.3
	Sequence conflict	288 – 307	20	YLRLV…GYAEE → GNGEGRRPQRYTKPGAEKAK in AAI11574. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q8TDR2 [UniParc]. Last modified March 23, 2010. Version 2. Checksum: CA1A99D26829C9AF	FASTA	534	58,051
	10 20 30 40 50 60 MGHQESPLAR APAGGAAYVK RLCKGLSWRE HVESHGSLGA QASPASAAAA EGSATRRARA 70 80 90 100 110 120 ATSRAARSRR QPGPGADHPQ AGAPGGKRAA RKWRCAGQVT IQGPAPPRPR AGRRDEAGGA 130 140 150 160 170 180 RAAPLLLPPP PAAMETGKDG ARRGTQSPER KRRSPVPRAP STKLRPAAAA RAMDPVAAEA 190 200 210 220 230 240 PGEAFLARRR PEGGGGSARP RYSLLAEIGR GSYGVVYEAV AGRSGARVAV KKIRCDAPEN 250 260 270 280 290 300 VELALAEFWA LTSLKRRHQN VVQFEECVLQ RNGLAQRMSH GNKSSQLYLR LVETSLKGER 310 320 330 340 350 360 ILGYAEEPCY LWFVMEFCEG GDLNQYVLSR RPDPATNKSF MLQLTSAIAF LHKNHIVHRD 370 380 390 400 410 420 LKPDNILITE RSGTPILKVA DFGLSKVCAG LAPRGKEGNQ DNKNVNVNKY WLSSACGSDF 430 440 450 460 470 480 YMAPEVWEGH YTAKADIFAL GIIIWAMIER ITFIDSETKK ELLGTYIKQG TEIVPVGEAL 490 500 510 520 530 LENPKMELHI PQKRRTSMSE GIKQLLKDML AANPQDRPDA FELETRMDQV TCAA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identifying and characterizing a novel protein kinase STK35L1 and deciphering its orthologs and close-homologs in vertebrates." Goyal P., Behring A., Kumar A., Siess W. PLoS ONE 4:E6981-E6981(2009) [PubMed: 19756140] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
[2]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed: 11780052] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 51-534. Tissue: Cerebellum.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 101-534. Tissue: Skin.
[5]	"Clik1: a novel kinase targeted to actin stress fibers by the CLP-36 PDZ-LIM protein." Vallenius T., Maekelae T.P. J. Cell Sci. 115:2067-2073(2002) [PubMed: 11973348] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 134-534, MUTAGENESIS OF LYS-231, SUBCELLULAR LOCATION, AUTOPHOSPHORYLATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	GQ281297 mRNA. Translation: ACU33925.1. AL359916 Genomic DNA. Translation: CAI15590.1. Sequence problems. AK314728 mRNA. Translation: BAG37270.1. Different initiation. BC017340 mRNA. Translation: AAH17340.2. BC111573 mRNA. Translation: AAI11574.1. BC140883 mRNA. Translation: AAI40884.1. Different initiation. BC140884 mRNA. Translation: AAI40885.1. Different initiation. AF195026 mRNA. Translation: AAL99353.1.
IPI	IPI00291457.
RefSeq	NP_543026.2. NM_080836.3.
UniGene	Hs.100057.
3D structure databases
ProteinModelPortal	Q8TDR2.
SMR	Q8TDR2. Positions 201-525.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8TDR2.
PTM databases
PhosphoSite	Q8TDR2.
Polymorphism databases
DMDM	292495039.
Proteomic databases
PRIDE	Q8TDR2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000246032; ENSP00000246032; ENSG00000125834. ENST00000381482; ENSP00000370891; ENSG00000125834.
GeneID	140901.
KEGG	hsa:140901.
UCSC	uc002wfw.2. human.
Organism-specific databases
CTD	140901.
GeneCards	GC20P002082.
H-InvDB	HIX0040513.
HGNC	HGNC:16254. STK35.
MIM	609370. gene.
neXtProt	NX_Q8TDR2.
PharmGKB	PA38097.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG10177.
GeneTree	ENSGT00570000078909.
HOGENOM	HBG402936.
HOVERGEN	HBG056829.
InParanoid	Q8TDR2.
OrthoDB	EOG47D9G4.
Gene expression databases
ArrayExpress	Q8TDR2.
Bgee	Q8TDR2.
CleanEx	HS_STK35.
Genevestigator	Q8TDR2.
GermOnline	ENSG00000125834. Homo sapiens.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
KO	K08859.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	84567.
SOURCE	Search...

Entry information

Entry name

STK35_HUMAN

Accession

Primary (citable) accession number: Q8TDR2
Secondary accession number(s): B2RBM3 , C7ENV8, Q2NKW6, Q5T3R1, Q5T3R2, Q96AB4, Q9BZ06

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 27, 2003
Last sequence update:	March 23, 2010
Last modified:	December 14, 2011
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.