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Q8TDR2 (STK35_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein kinase 35

EC=2.7.11.1
Alternative name(s):
CLP-36-interacting kinase 1
Short name=CLIK-1
PDLIM1-interacting kinase 1
Serine/threonine-protein kinase 35 L1
Gene names
Name:STK35
Synonyms:CLIK1, PDIK1, STK35L1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length534 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subunit structure

Interacts with PDLIM1/CLP-36.

Subcellular location

Nucleus. Nucleusnucleolus. Cytoplasm. Note: When associated with PDLIM1, it is mostly found in cytoplasm, localized to actin stress fibers (Ref.5). However, Ref.1 detected STK35 only in the nucleus, and the presence of PDLIM1 had no influence on its location. Ref.1 Ref.5

Tissue specificity

Expressed in testis.

Post-translational modification

Autophosphorylated. Ref.5

Miscellaneous

Association with PDLIM1 is controversial (Ref.5).

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Contains 1 protein kinase domain.

Sequence caution

The sequence AAI40884.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence AAI40885.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence BAG37270.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence CAI15590.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Cellular componentCytoplasm
Nucleus
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleolus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein serine/threonine kinase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 534534Serine/threonine-protein kinase 35
PRO_0000086717

Regions

Domain202 – 530329Protein kinase
Nucleotide binding208 – 2169ATP By similarity
Compositional bias40 – 187148Ala-rich

Sites

Active site3601Proton acceptor By similarity
Binding site2311ATP Probable

Experimental info

Mutagenesis2311K → M: No autophosphorylation. Ref.5
Sequence conflict691R → G in BAG37270. Ref.3
Sequence conflict1151D → G in BAG37270. Ref.3
Sequence conflict288 – 30720YLRLV…GYAEE → GNGEGRRPQRYTKPGAEKAK in AAI11574. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q8TDR2 [UniParc].

Last modified March 23, 2010. Version 2.
Checksum: CA1A99D26829C9AF

FASTA53458,051
        10         20         30         40         50         60 
MGHQESPLAR APAGGAAYVK RLCKGLSWRE HVESHGSLGA QASPASAAAA EGSATRRARA 

        70         80         90        100        110        120 
ATSRAARSRR QPGPGADHPQ AGAPGGKRAA RKWRCAGQVT IQGPAPPRPR AGRRDEAGGA 

       130        140        150        160        170        180 
RAAPLLLPPP PAAMETGKDG ARRGTQSPER KRRSPVPRAP STKLRPAAAA RAMDPVAAEA 

       190        200        210        220        230        240 
PGEAFLARRR PEGGGGSARP RYSLLAEIGR GSYGVVYEAV AGRSGARVAV KKIRCDAPEN 

       250        260        270        280        290        300 
VELALAEFWA LTSLKRRHQN VVQFEECVLQ RNGLAQRMSH GNKSSQLYLR LVETSLKGER 

       310        320        330        340        350        360 
ILGYAEEPCY LWFVMEFCEG GDLNQYVLSR RPDPATNKSF MLQLTSAIAF LHKNHIVHRD 

       370        380        390        400        410        420 
LKPDNILITE RSGTPILKVA DFGLSKVCAG LAPRGKEGNQ DNKNVNVNKY WLSSACGSDF 

       430        440        450        460        470        480 
YMAPEVWEGH YTAKADIFAL GIIIWAMIER ITFIDSETKK ELLGTYIKQG TEIVPVGEAL 

       490        500        510        520        530 
LENPKMELHI PQKRRTSMSE GIKQLLKDML AANPQDRPDA FELETRMDQV TCAA 

« Hide

References

« Hide 'large scale' references
[1]"Identifying and characterizing a novel protein kinase STK35L1 and deciphering its orthologs and close-homologs in vertebrates."
Goyal P., Behring A., Kumar A., Siess W.
PLoS ONE 4:E6981-E6981(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
[2]"The DNA sequence and comparative analysis of human chromosome 20."
Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. expand/collapse author list , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 51-534.
Tissue: Cerebellum.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 101-534.
Tissue: Skin.
[5]"Clik1: a novel kinase targeted to actin stress fibers by the CLP-36 PDZ-LIM protein."
Vallenius T., Maekelae T.P.
J. Cell Sci. 115:2067-2073(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 134-534, MUTAGENESIS OF LYS-231, SUBCELLULAR LOCATION, AUTOPHOSPHORYLATION.
[6]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
GQ281297 mRNA. Translation: ACU33925.1.
AL359916 Genomic DNA. Translation: CAI15590.1. Sequence problems.
AK314728 mRNA. Translation: BAG37270.1. Different initiation.
BC017340 mRNA. Translation: AAH17340.2.
BC111573 mRNA. Translation: AAI11574.1.
BC140883 mRNA. Translation: AAI40884.1. Different initiation.
BC140884 mRNA. Translation: AAI40885.1. Different initiation.
AF195026 mRNA. Translation: AAL99353.1.
CCDSCCDS13024.2.
RefSeqNP_543026.2. NM_080836.3.
UniGeneHs.100057.

3D structure databases

ProteinModelPortalQ8TDR2.
SMRQ8TDR2. Positions 201-521.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid126760. 3 interactions.
STRING9606.ENSP00000246032.

Chemistry

BindingDBQ8TDR2.
ChEMBLCHEMBL5651.
GuidetoPHARMACOLOGY2222.

PTM databases

PhosphoSiteQ8TDR2.

Polymorphism databases

DMDM292495039.

Proteomic databases

MaxQBQ8TDR2.
PaxDbQ8TDR2.
PRIDEQ8TDR2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000246032; ENSP00000246032; ENSG00000125834.
ENST00000381482; ENSP00000370891; ENSG00000125834.
ENST00000493263; ENSP00000426612; ENSG00000125834.
GeneID140901.
KEGGhsa:140901.
UCSCuc002wfw.4. human.

Organism-specific databases

CTD140901.
GeneCardsGC20P002082.
H-InvDBHIX0040513.
HIX0040701.
HGNCHGNC:16254. STK35.
HPAHPA051673.
MIM609370. gene.
neXtProtNX_Q8TDR2.
PharmGKBPA38097.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0515.
HOVERGENHBG056829.
InParanoidQ8TDR2.
KOK08859.
OMAPRGKEGH.
OrthoDBEOG76DTSN.
PhylomeDBQ8TDR2.
TreeFamTF105336.

Enzyme and pathway databases

SignaLinkQ8TDR2.

Gene expression databases

ArrayExpressQ8TDR2.
BgeeQ8TDR2.
CleanExHS_STK35.
GenevestigatorQ8TDR2.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 2 hits.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiSerine/threonine_kinase_35.
STK35L1.
GenomeRNAi140901.
NextBio84567.
PROQ8TDR2.
SOURCESearch...

Entry information

Entry nameSTK35_HUMAN
AccessionPrimary (citable) accession number: Q8TDR2
Secondary accession number(s): B2RBM3 expand/collapse secondary AC list , C7ENV8, Q2NKW6, Q5T3R1, Q5T3R2, Q96AB4, Q9BZ06
Entry history
Integrated into UniProtKB/Swiss-Prot: January 27, 2003
Last sequence update: March 23, 2010
Last modified: July 9, 2014
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 20

Human chromosome 20: entries, gene names and cross-references to MIM