Reviewed, UniProtKB/Swiss-Prot Q8TDR2 (STK35_HUMAN)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase 35
    EC=2.7.11.1
Alternative name(s):
    CLP-36-interacting kinase
    Clik1
    PDLIM1-interacting kinase 1

Gene names

Name:	STK35
Synonyms:	CLIK1, PDIK1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	401 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Interacts with PDLIM1/CLP-36.
Subcellular location	Nucleus. Cytoplasm. Note: When associated with PDLIM1, it is mostly found in cytoplasm, localized to actin stress fibers.
Tissue specificity	Expressed in testis.
Post-translational modification	Autophosphorylated. Ref.1
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 401	401	Serine/threonine-protein kinase 35		PRO_0000086717
Regions
	Domain	69 – 397	329	Protein kinase
	Nucleotide binding	75 – 83	9	ATP By similarity
	Compositional bias	34 – 39	6	Poly-Ala
Sites
	Active site	227	1	Proton acceptor By similarity
	Binding site	98	1	ATP By similarity
Experimental info
	Mutagenesis	98	1	K → M: No autophosphorylation. Ref.1
	Sequence conflict	155 – 174	20	YLRLV…GYAEE → GNGEGRRPQRYTKPGAEKAK in AAI11574. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q8TDR2-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 425B958ECA7BA016	FASTA	401	44,573
	10 20 30 40 50 60 METGKDGARR GTQSPERKRR SPVPRAPSTK LRPAAAARAM DPVAAEAPGE AFLARRRPEG 70 80 90 100 110 120 GGGSARPRYS LLAEIGRGSY GVVYEAVAGR SGARVAVKKI RCDAPENVEL ALAEFWALTS 130 140 150 160 170 180 LKRRHQNVVQ FEECVLQRNG LAQRMSHGNK SSQLYLRLVE TSLKGERILG YAEEPCYLWF 190 200 210 220 230 240 VMEFCEGGDL NQYVLSRRPD PATNKSFMLQ LTSAIAFLHK NHIVHRDLKP DNILITERSG 250 260 270 280 290 300 TPILKVADFG LSKVCAGLAP RGKEGNQDNK NVNVNKYWLS SACGSDFYMA PEVWEGHYTA 310 320 330 340 350 360 KADIFALGII IWAMIERITF IDSETKKELL GTYIKQGTEI VPVGEALLEN PKMELHIPQK 370 380 390 400 RRTSMSEGIK QLLKDMLAAN PQDRPDAFEL ETRMDQVTCA A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Clik1: a novel kinase targeted to actin stress fibers by the CLP-36 PDZ-LIM protein." Vallenius T., Maekelae T.P. J. Cell Sci. 115:2067-2073(2002) [PubMed: 11973348] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF LYS-98, AUTOPHOSPHORYLATION.
[2]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed: 11780052] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 7-401. Tissue: Skin.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF195026 mRNA. Translation: AAL99353.1. AL359916 Genomic DNA. Translation: CAI15590.1. BC017340 mRNA. Translation: AAH17340.2. BC111573 mRNA. Translation: AAI11574.1.
IPI	IPI00291457.
RefSeq	NP_543026.1.
UniGene	Hs.100057
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q8TDR2.
Proteomic databases
PRIDE	Q8TDR2.
Genome annotation databases
Ensembl	ENSG00000125834. Homo sapiens. [Contig view]
GeneID	140901.
KEGG	hsa:140901.
Organism-specific databases
GeneCards	GC20P002030.
H-InvDB	HIX0040513.
HGNC	HGNC:16254. STK35.
MIM	609370. gene.
PharmGKB	PA38097.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q8TDR2.
HOVERGEN	Q8TDR2.
OMA	Q8TDR2. METGEED.
Enzyme and pathway databases
BRENDA	2.7.11.1. 247.
Gene expression databases
ArrayExpress	Q8TDR2.
Bgee	Q8TDR2.
CleanEx	HS_STK35.
GermOnline	ENSG00000125834. Homo sapiens.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	84567.
SOURCE	Search...

Entry information

Entry name

STK35_HUMAN

Accession

Primary (citable) accession number: Q8TDR2
Secondary accession number(s): Q2NKW6 , Q5T3R1, Q5T3R2, Q96AB4, Q9BZ06

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 27, 2003
Last sequence update:	June 1, 2002
Last modified:	June 16, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)