Reviewed,
UniProtKB/Swiss-Prot Q8TDR2 (STK35_HUMAN)
Last modified
June 16, 2009.
Version 71.
History...
Clusters with 100%,
90%,
50% identity |
Documents (4) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Serine/threonine-protein kinase 35 EC=2.7.11.1 Alternative name(s): CLP-36-interacting kinase Clik1 PDLIM1-interacting kinase 1 | ||||
| Gene names |
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| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 401 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Catalytic activity | ATP + a protein = ADP + a phosphoprotein. |
| Subunit structure | Interacts with PDLIM1/CLP-36. |
| Subcellular location | Nucleus. Cytoplasm. Note: When associated with PDLIM1, it is mostly found in cytoplasm, localized to actin stress fibers. |
| Tissue specificity | Expressed in testis. |
| Post-translational modification | Autophosphorylated. Ref.1 |
| Sequence similarities | Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cytoplasm Nucleus |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Kinase Serine/threonine-protein kinase Transferase |
| PTM | Phosphoprotein |
| Gene Ontology (GO) | |
| Biological process | protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleusInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activityInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 401 | 401 | Serine/threonine-protein kinase 35 | PRO_0000086717 | |||||
Regions | |||||||||
| Domain | 69 – 397 | 329 | Protein kinase | ||||||
| Nucleotide binding | 75 – 83 | 9 | ATP By similarity | ||||||
| Compositional bias | 34 – 39 | 6 | Poly-Ala | ||||||
Sites | |||||||||
| Active site | 227 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 98 | 1 | ATP By similarity | ||||||
Experimental info | |||||||||
| Mutagenesis | 98 | 1 | K → M: No autophosphorylation. Ref.1 | ||||||
| Sequence conflict | 155 – 174 | 20 | YLRLV…GYAEE → GNGEGRRPQRYTKPGAEKAK in AAI11574. Ref.3 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Clik1: a novel kinase targeted to actin stress fibers by the CLP-36 PDZ-LIM protein." Vallenius T., Maekelae T.P. J. Cell Sci. 115:2067-2073(2002) [PubMed: 11973348] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF LYS-98, AUTOPHOSPHORYLATION. |
| [2] | "The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. Rogers J.Nature 414:865-871(2001) [PubMed: 11780052] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [3] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 7-401. Tissue: Skin. |
Cross-references
Sequence databases | |
|---|---|
| AF195026 mRNA. Translation: AAL99353.1. AL359916 Genomic DNA. Translation: CAI15590.1. BC017340 mRNA. Translation: AAH17340.2. BC111573 mRNA. Translation: AAI11574.1. | |
| IPI | IPI00291457. |
| RefSeq | NP_543026.1. |
| UniGene | Hs.100057 |
3D structure databases | |
| ModBase | Search... |
PTM databases | |
| PhosphoSite | Q8TDR2. |
Proteomic databases | |
| PRIDE | Q8TDR2. |
Genome annotation databases | |
| Ensembl | ENSG00000125834. Homo sapiens. [Contig view] |
| GeneID | 140901. |
| KEGG | hsa:140901. |
Organism-specific databases | |
| GeneCards | GC20P002030. |
| H-InvDB | HIX0040513. |
| HGNC | HGNC:16254. STK35. |
| MIM | 609370. gene. |
| PharmGKB | PA38097. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | Q8TDR2. |
| HOVERGEN | Q8TDR2. |
| OMA | Q8TDR2. METGEED. |
Enzyme and pathway databases | |
| BRENDA | 2.7.11.1. 247. |
Gene expression databases | |
| ArrayExpress | Q8TDR2. |
| Bgee | Q8TDR2. |
| CleanEx | HS_STK35. |
| GermOnline | ENSG00000125834. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. [Graphical view] |
| Pfam | PF00069. Pkinase. 1 hit. [Graphical view] |
| ProDom | PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| PROSITE | PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 84567. |
| SOURCE | Search... |
Entry information
| Entry name | STK35_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q8TDR2 Secondary accession number(s): Q2NKW6 Q9BZ06 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 20 Human chromosome 20: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| Human and mouse protein kinases Human and mouse protein kinases: classification and index |
| SIMILARITY comments Index of protein domains and families |

Clusters with


