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Protein

Glucosamine-6-phosphate isomerase 2

Gene

GNPDA2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Alpha-D-glucosamine 6-phosphate + H2O = D-fructose 6-phosphate + NH3.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei72 – 721Proton acceptor; for enolization stepBy similarity
Active sitei141 – 1411For ring-opening stepBy similarity
Active sitei143 – 1431Proton acceptor; for ring-opening stepBy similarity
Active sitei148 – 1481For ring-opening stepBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

BRENDAi3.5.99.6. 2681.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucosamine-6-phosphate isomerase 2 (EC:3.5.99.6)
Alternative name(s):
Glucosamine-6-phosphate deaminase 2
Short name:
GNPDA 2
Short name:
GlcN6P deaminase 2
Glucosamine-6-phosphate isomerase SB52
Gene namesi
Name:GNPDA2
Synonyms:GNP2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:21526. GNPDA2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134939177.

Polymorphism and mutation databases

DMDMi74723936.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 276276Glucosamine-6-phosphate isomerase 2PRO_0000343205Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei64 – 641N6-acetyllysineBy similarity
Modified residuei161 – 1611PhosphothreonineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8TDQ7.
MaxQBiQ8TDQ7.
PaxDbiQ8TDQ7.
PRIDEiQ8TDQ7.
TopDownProteomicsiQ8TDQ7-2. [Q8TDQ7-2]

PTM databases

iPTMnetiQ8TDQ7.
PhosphoSiteiQ8TDQ7.

Expressioni

Gene expression databases

BgeeiQ8TDQ7.
CleanExiHS_GNPDA2.
ExpressionAtlasiQ8TDQ7. baseline and differential.
GenevisibleiQ8TDQ7. HS.

Organism-specific databases

HPAiHPA000499.
HPA046891.

Interactioni

Subunit structurei

Homohexamer.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
AMDHD2Q9Y3033EBI-10275006,EBI-2798672
TRIM27P143733EBI-10275006,EBI-719493

Protein-protein interaction databases

BioGridi126335. 14 interactions.
IntActiQ8TDQ7. 2 interactions.
STRINGi9606.ENSP00000295448.

Structurei

3D structure databases

ProteinModelPortaliQ8TDQ7.
SMRiQ8TDQ7. Positions 1-274.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili105 – 13026Sequence analysisAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3148. Eukaryota.
COG0363. LUCA.
GeneTreeiENSGT00390000014316.
HOGENOMiHOG000064979.
HOVERGENiHBG002546.
InParanoidiQ8TDQ7.
KOiK02564.
OMAiNRIIQFR.
OrthoDBiEOG7NW69T.
PhylomeDBiQ8TDQ7.
TreeFamiTF300841.

Family and domain databases

HAMAPiMF_01241. GlcN6P_deamin.
InterProiIPR006148. Glc/Gal-6P_isomerase.
IPR004547. Glucosamine6P_isomerase.
IPR018321. Glucosamine6P_isomerase_CS.
[Graphical view]
PANTHERiPTHR11280. PTHR11280. 1 hit.
PfamiPF01182. Glucosamine_iso. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00502. nagB. 1 hit.
PROSITEiPS01161. GLC_GALNAC_ISOMERASE. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8TDQ7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRLVILDNYD LASEWAAKYI CNRIIQFKPG QDRYFTLGLP TGSTPLGCYK
60 70 80 90 100
KLIEYHKNGH LSFKYVKTFN MDEYVGLPRN HPESYHSYMW NNFFKHIDID
110 120 130 140 150
PNNAHILDGN AADLQAECDA FENKIKEAGG IDLFVGGIGP DGHIAFNEPG
160 170 180 190 200
SSLVSRTRLK TLAMDTILAN AKYFDGDLSK VPTMALTVGV GTVMDAREVM
210 220 230 240 250
ILITGAHKAF ALYKAIEEGV NHMWTVSAFQ QHPRTIFVCD EDATLELRVK
260 270
TVKYFKGLMH VHNKLVDPLF SMKDGN
Length:276
Mass (Da):31,085
Last modified:June 1, 2002 - v1
Checksum:i2BCFD72F734639D0
GO
Isoform 2 (identifier: Q8TDQ7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     217-217: Missing.

Show »
Length:275
Mass (Da):30,956
Checksum:i6DB65CA86569D445
GO
Isoform 3 (identifier: Q8TDQ7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     258-276: LMHVHNKLVDPLFSMKDGN → EH

Show »
Length:259
Mass (Da):29,173
Checksum:i1C0DB9A7FFDB5C92
GO
Isoform 4 (identifier: Q8TDQ7-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-70: Missing.

Show »
Length:206
Mass (Da):22,912
Checksum:iE3D8A72A584D3F06
GO
Isoform 5 (identifier: Q8TDQ7-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     43-76: Missing.

Show »
Length:242
Mass (Da):27,091
Checksum:iBA5F01D6E1F5C015
GO

Sequence cautioni

The sequence BAD93141.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti37 – 371L → P in BAB70977 (PubMed:14702039).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti182 – 1821P → S.1 Publication
Corresponds to variant rs17851302 [ dbSNP | Ensembl ].
VAR_044348

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 7070Missing in isoform 4. 1 PublicationVSP_047033Add
BLAST
Alternative sequencei43 – 7634Missing in isoform 5. 1 PublicationVSP_047034Add
BLAST
Alternative sequencei217 – 2171Missing in isoform 2. 1 PublicationVSP_034579
Alternative sequencei258 – 27619LMHVH…MKDGN → EH in isoform 3. 1 PublicationVSP_034580Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF247786 mRNA. Translation: AAL95691.1.
AY173948 mRNA. Translation: AAO49718.1.
AK055639 mRNA. Translation: BAB70977.1.
AK296051 mRNA. Translation: BAG58815.1.
AB209904 mRNA. Translation: BAD93141.1. Different initiation.
AC096586 Genomic DNA. No translation available.
CH471069 Genomic DNA. Translation: EAW93019.1.
BC015532 mRNA. Translation: AAH15532.1.
AL834506 mRNA. Translation: CAD39163.1.
CCDSiCCDS3469.1. [Q8TDQ7-1]
CCDS59472.1. [Q8TDQ7-4]
CCDS59473.1. [Q8TDQ7-5]
RefSeqiNP_001257809.1. NM_001270880.1. [Q8TDQ7-5]
NP_001257810.1. NM_001270881.1. [Q8TDQ7-4]
NP_612208.1. NM_138335.2. [Q8TDQ7-1]
UniGeneiHs.21398.
Hs.597934.
Hs.619562.

Genome annotation databases

EnsembliENST00000295448; ENSP00000295448; ENSG00000163281. [Q8TDQ7-1]
ENST00000507534; ENSP00000427423; ENSG00000163281. [Q8TDQ7-4]
ENST00000507917; ENSP00000425868; ENSG00000163281. [Q8TDQ7-5]
ENST00000509756; ENSP00000424061; ENSG00000163281. [Q8TDQ7-3]
GeneIDi132789.
KEGGihsa:132789.
UCSCiuc003gwy.5. human. [Q8TDQ7-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF247786 mRNA. Translation: AAL95691.1.
AY173948 mRNA. Translation: AAO49718.1.
AK055639 mRNA. Translation: BAB70977.1.
AK296051 mRNA. Translation: BAG58815.1.
AB209904 mRNA. Translation: BAD93141.1. Different initiation.
AC096586 Genomic DNA. No translation available.
CH471069 Genomic DNA. Translation: EAW93019.1.
BC015532 mRNA. Translation: AAH15532.1.
AL834506 mRNA. Translation: CAD39163.1.
CCDSiCCDS3469.1. [Q8TDQ7-1]
CCDS59472.1. [Q8TDQ7-4]
CCDS59473.1. [Q8TDQ7-5]
RefSeqiNP_001257809.1. NM_001270880.1. [Q8TDQ7-5]
NP_001257810.1. NM_001270881.1. [Q8TDQ7-4]
NP_612208.1. NM_138335.2. [Q8TDQ7-1]
UniGeneiHs.21398.
Hs.597934.
Hs.619562.

3D structure databases

ProteinModelPortaliQ8TDQ7.
SMRiQ8TDQ7. Positions 1-274.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi126335. 14 interactions.
IntActiQ8TDQ7. 2 interactions.
STRINGi9606.ENSP00000295448.

PTM databases

iPTMnetiQ8TDQ7.
PhosphoSiteiQ8TDQ7.

Polymorphism and mutation databases

DMDMi74723936.

Proteomic databases

EPDiQ8TDQ7.
MaxQBiQ8TDQ7.
PaxDbiQ8TDQ7.
PRIDEiQ8TDQ7.
TopDownProteomicsiQ8TDQ7-2. [Q8TDQ7-2]

Protocols and materials databases

DNASUi132789.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000295448; ENSP00000295448; ENSG00000163281. [Q8TDQ7-1]
ENST00000507534; ENSP00000427423; ENSG00000163281. [Q8TDQ7-4]
ENST00000507917; ENSP00000425868; ENSG00000163281. [Q8TDQ7-5]
ENST00000509756; ENSP00000424061; ENSG00000163281. [Q8TDQ7-3]
GeneIDi132789.
KEGGihsa:132789.
UCSCiuc003gwy.5. human. [Q8TDQ7-1]

Organism-specific databases

CTDi132789.
GeneCardsiGNPDA2.
HGNCiHGNC:21526. GNPDA2.
HPAiHPA000499.
HPA046891.
MIMi613222. gene.
neXtProtiNX_Q8TDQ7.
PharmGKBiPA134939177.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3148. Eukaryota.
COG0363. LUCA.
GeneTreeiENSGT00390000014316.
HOGENOMiHOG000064979.
HOVERGENiHBG002546.
InParanoidiQ8TDQ7.
KOiK02564.
OMAiNRIIQFR.
OrthoDBiEOG7NW69T.
PhylomeDBiQ8TDQ7.
TreeFamiTF300841.

Enzyme and pathway databases

BRENDAi3.5.99.6. 2681.

Miscellaneous databases

GeneWikiiGNPDA2.
GenomeRNAii132789.
NextBioi35472517.
PROiQ8TDQ7.
SOURCEiSearch...

Gene expression databases

BgeeiQ8TDQ7.
CleanExiHS_GNPDA2.
ExpressionAtlasiQ8TDQ7. baseline and differential.
GenevisibleiQ8TDQ7. HS.

Family and domain databases

HAMAPiMF_01241. GlcN6P_deamin.
InterProiIPR006148. Glc/Gal-6P_isomerase.
IPR004547. Glucosamine6P_isomerase.
IPR018321. Glucosamine6P_isomerase_CS.
[Graphical view]
PANTHERiPTHR11280. PTHR11280. 1 hit.
PfamiPF01182. Glucosamine_iso. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00502. nagB. 1 hit.
PROSITEiPS01161. GLC_GALNAC_ISOMERASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and functional characterization of GNPI2, a novel human homolog of glucosamine-6-phosphate isomerase/oscillin."
    Zhang J., Zhang W., Zou D., Chen G., Wan T., Li N., Cao X.
    J. Cell. Biochem. 88:932-940(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Characterization of a novel putative glucosamine-6-phosphate isomerase."
    Xu J., Xie Y., Mao Y.
    Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5).
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4).
    Tissue: Subthalamic nucleus.
  4. Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Aortic endothelium.
  5. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT SER-182.
    Tissue: Uterus.
  8. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 120-276 (ISOFORM 1).
    Tissue: Kidney.
  9. "Two mammalian glucosamine-6-phosphate deaminases: a structural and genetic study."
    Arreola R., Valderrama B., Morante M.L., Horjales E.
    FEBS Lett. 551:63-70(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.

Entry informationi

Entry nameiGNPI2_HUMAN
AccessioniPrimary (citable) accession number: Q8TDQ7
Secondary accession number(s): B4DJF3
, Q2VYF1, Q59EA7, Q8NCZ8, Q96BJ4, Q96NC6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: June 1, 2002
Last modified: March 16, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.