Skip Header

Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot Q8TDN7 (ACER1_HUMAN)

Last modified February 9, 2010. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Alkaline ceramidase 1
      Short name=Alkaline CDase 1
      Short name=AlkCDase 1
    EC=3.5.1.23
Alternative name(s):
    N-acylsphingosine amidohydrolase 3
    Acylsphingosine deacylase 3
Gene names
Name: ACER1
Synonyms: ASAH3
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length264 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Function

Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 8.0. Has a highly restricted substrate specificity for the natural stereoisomer of ceramide with D-erythro-sphingosine but not D-ribo-phytosphingosine or D-erythro-dihydrosphingosine as a backbone. May have a role in regulating the levels of bioactive lipids ceramide and sphingosine 1-phosphate, as well as complex sphingolipids By similarity.

Catalytic activity

N-acylsphingosine + H2O = a carboxylate + sphingosine.

Enzyme regulation

Inhibited by sphingosine By similarity.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.

Tissue specificity

Mainly expressed in epidermis. Ref.3

Sequence similarities

Belongs to the alkaline ceramidase family.

Ontologies

Keywords
   Biological processLipid metabolism
   Cellular componentEndoplasmic reticulum
Membrane
   DomainTransmembrane
   Molecular functionHydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular componentGolgi membrane

Inferred from electronic annotation. Source: InterPro

endoplasmic reticulum membrane

Inferred from Experiment. Source: Reactome

integral to membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionceramidase activity

Inferred from Experiment. Source: Reactome

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 264264Alkaline ceramidase 1
PRO_0000247745

Regions

Transmembrane28 – 4821 Potential
Transmembrane58 – 7821 Potential
Transmembrane82 – 10221 Potential
Transmembrane120 – 13718 Potential
Transmembrane139 – 15921 Potential
Transmembrane177 – 19721 Potential
Transmembrane207 – 22721 Potential

Amino acid modifications

Glycosylation1161N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q8TDN7-1 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: E16E5DB81D064F60

FASTA26431,095
        10         20         30         40         50         60 
MPSIFAYQSS EVDWCESNFQ YSELVAEFYN TFSNIPFFIF GPLMMLLMHP YAQKRSRYIY 

        70         80         90        100        110        120 
VVWVLFMIIG LFSMYFHMTL SFLGQLLDEI AILWLLGSGY SIWMPRCYFP SFLGGNRSQF 

       130        140        150        160        170        180 
IRLVFITTVV STLLSFLRPT VNAYALNSIA LHILYIVCQE YRKTSNKELR HLIEVSVVLW 

       190        200        210        220        230        240 
AVALTSWISD RLLCSFWQRI HFFYLHSIWH VLISITFPYG MVTMALVDAN YEMPGETLKV 

       250        260 
RYWPRDSWPV GLPYVEIRGD DKDC 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of a mouse endoplasmic reticulum alkaline ceramidase: an enzyme that preferentially regulates metabolism of very long chain ceramides."
Mao C., Xu R., Szulc Z.M., Bielawski J., Becker K.P., Bielawska A., Galadari S.H., Hu W., Obeid L.M.
J. Biol. Chem. 278:31184-31191(2003) [PubMed: 12783875] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]"Differentiation-associated expression of ceramidase isoforms in cultured keratinocytes and epidermis."
Houben E., Holleran W.M., Yaginuma T., Mao C., Obeid L.M., Rogiers V., Takagi Y., Elias P.M., Uchida Y.
J. Lipid Res. 47:1063-1070(2006) [PubMed: 16477081] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF347024 mRNA. Translation: AAL83822.1.
BC112122 mRNA. Translation: AAI12123.1.
BC112124 mRNA. Translation: AAI12125.1.
IPIIPI00152570.
RefSeqNP_597999.1.
UniGeneHs.352609

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGQ8TDN7.

Genome annotation databases

EnsemblENST00000301452; ENSP00000301452; ENSG00000167769; Homo sapiens. [Genome view]
GeneID125981.
KEGGhsa:125981.
UCSCuc002mel.1. human.

Organism-specific databases

CTD125981.
GeneCardsGC19M006259.
HGNCHGNC:18356. ACER1.
PharmGKBPA134897634.
GenAtlasSearch...

Phylogenomic databases

eggNOGprNOG04896.
HOGENOMHBG446871.
HOVERGENQ8TDN7.
InParanoidQ8TDN7.
OMAFQYSELV.
OrthoDBEOG9M682S.
PhylomeDBQ8TDN7.

Enzyme and pathway databases

BRENDA3.5.1.23. 247.
ReactomeREACT_602. Metabolism of lipids and lipoproteins.

Gene expression databases

ArrayExpressQ8TDN7.
BgeeQ8TDN7.
CleanExHS_ACER1.
GenevestigatorQ8TDN7.
GermOnlineENSG00000167769. Homo sapiens.

Family and domain databases

ProtoNetSearch...

Other Resources

NextBio81606.

Entry information

Entry nameACER1_HUMAN
AccessionPrimary (citable) accession number: Q8TDN7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: June 1, 2002
Last modified: February 9, 2010
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents