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Protein

Ribosome biogenesis protein BRX1 homolog

Gene

BRIX1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for biogenesis of the 60S ribosomal subunit.

GO - Molecular functioni

  • poly(A) RNA binding Source: UniProtKB
  • RNA binding Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ribosome biogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosome biogenesis protein BRX1 homolog
Alternative name(s):
Brix domain-containing protein 2
Gene namesi
Name:BRIX1
Synonyms:BRIX, BXDC2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:24170. BRIX1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA165660166.

Polymorphism and mutation databases

BioMutaiBRIX1.
DMDMi30580352.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 353353Ribosome biogenesis protein BRX1 homologPRO_0000120230Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei276 – 2761N6-acetyllysineCombined sources
Cross-linki322 – 322Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiQ8TDN6.
MaxQBiQ8TDN6.
PaxDbiQ8TDN6.
PeptideAtlasiQ8TDN6.
PRIDEiQ8TDN6.

PTM databases

iPTMnetiQ8TDN6.
PhosphoSiteiQ8TDN6.

Expressioni

Gene expression databases

BgeeiQ8TDN6.
CleanExiHS_BXDC2.
GenevisibleiQ8TDN6. HS.

Organism-specific databases

HPAiHPA039614.

Interactioni

Protein-protein interaction databases

BioGridi120586. 111 interactions.
IntActiQ8TDN6. 23 interactions.
MINTiMINT-1373627.
STRINGi9606.ENSP00000338862.

Structurei

3D structure databases

ProteinModelPortaliQ8TDN6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini60 – 249190BrixPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the BRX1 family.Curated
Contains 1 Brix domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2971. Eukaryota.
COG5154. LUCA.
GeneTreeiENSGT00390000014467.
HOGENOMiHOG000212937.
HOVERGENiHBG039185.
InParanoidiQ8TDN6.
KOiK14820.
OMAiKPKRNEK.
OrthoDBiEOG7Z95MP.
PhylomeDBiQ8TDN6.
TreeFamiTF105766.

Family and domain databases

InterProiIPR004154. Anticodon-bd.
IPR007109. Brix.
IPR026532. BRX1.
[Graphical view]
PANTHERiPTHR13634. PTHR13634. 1 hit.
PfamiPF04427. Brix. 1 hit.
[Graphical view]
SMARTiSM00879. Brix. 1 hit.
[Graphical view]
SUPFAMiSSF52954. SSF52954. 1 hit.
PROSITEiPS50833. BRIX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8TDN6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAATKRKRRG GFAVQAKKPK RNEIDAEPPA KRHATAEEVE EEERDRIPGP
60 70 80 90 100
VCKGKWKNKE RILIFSSRGI NFRTRHLMQD LRMLMPHSKA DTKMDRKDKL
110 120 130 140 150
FVINEVCEMK NCNKCIYFEA KKKQDLYMWL SNSPHGPSAK FLVQNIHTLA
160 170 180 190 200
ELKMTGNCLK GSRPLLSFDP AFDELPHYAL LKELLIQIFS TPRYHPKSQP
210 220 230 240 250
FVDHVFTFTI LDNRIWFRNF QIIEEDAALV EIGPRFVLNL IKIFQGSFGG
260 270 280 290 300
PTLYENPHYQ SPNMHRRVIR SITAAKYREK QQVKDVQKLR KKEPKTLLPH
310 320 330 340 350
DPTADVFVTP AEEKPIEIQW VKPEPKVDLK ARKKRIYKRQ RKMKQRMDSG

KTK
Length:353
Mass (Da):41,401
Last modified:May 9, 2003 - v2
Checksum:iF05597673A13B7CC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti11 – 2717GFAVQ…EIDAE → RLCSSGEEAKKKRNRCG in AAL83818 (PubMed:11843177).CuratedAdd
BLAST
Sequence conflicti34 – 341A → V in AAL83818 (PubMed:11843177).Curated
Sequence conflicti286 – 2861V → VP in AAL83818 (PubMed:11843177).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF347667 mRNA. Translation: AAL83818.1.
AY364244 mRNA. Translation: AAQ76803.1.
AK289610 mRNA. Translation: BAF82299.1.
CH471119 Genomic DNA. Translation: EAW55909.1.
BC001546 mRNA. Translation: AAH01546.2.
BC036741 mRNA. Translation: AAH36741.1.
CCDSiCCDS34143.1.
RefSeqiNP_060791.3. NM_018321.3.
UniGeneiHs.718510.

Genome annotation databases

EnsembliENST00000336767; ENSP00000338862; ENSG00000113460.
GeneIDi55299.
KEGGihsa:55299.
UCSCiuc003jja.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF347667 mRNA. Translation: AAL83818.1.
AY364244 mRNA. Translation: AAQ76803.1.
AK289610 mRNA. Translation: BAF82299.1.
CH471119 Genomic DNA. Translation: EAW55909.1.
BC001546 mRNA. Translation: AAH01546.2.
BC036741 mRNA. Translation: AAH36741.1.
CCDSiCCDS34143.1.
RefSeqiNP_060791.3. NM_018321.3.
UniGeneiHs.718510.

3D structure databases

ProteinModelPortaliQ8TDN6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120586. 111 interactions.
IntActiQ8TDN6. 23 interactions.
MINTiMINT-1373627.
STRINGi9606.ENSP00000338862.

PTM databases

iPTMnetiQ8TDN6.
PhosphoSiteiQ8TDN6.

Polymorphism and mutation databases

BioMutaiBRIX1.
DMDMi30580352.

Proteomic databases

EPDiQ8TDN6.
MaxQBiQ8TDN6.
PaxDbiQ8TDN6.
PeptideAtlasiQ8TDN6.
PRIDEiQ8TDN6.

Protocols and materials databases

DNASUi55299.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000336767; ENSP00000338862; ENSG00000113460.
GeneIDi55299.
KEGGihsa:55299.
UCSCiuc003jja.4. human.

Organism-specific databases

CTDi55299.
GeneCardsiBRIX1.
HGNCiHGNC:24170. BRIX1.
HPAiHPA039614.
neXtProtiNX_Q8TDN6.
PharmGKBiPA165660166.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2971. Eukaryota.
COG5154. LUCA.
GeneTreeiENSGT00390000014467.
HOGENOMiHOG000212937.
HOVERGENiHBG039185.
InParanoidiQ8TDN6.
KOiK14820.
OMAiKPKRNEK.
OrthoDBiEOG7Z95MP.
PhylomeDBiQ8TDN6.
TreeFamiTF105766.

Miscellaneous databases

GeneWikiiBXDC2.
GenomeRNAii55299.
NextBioi59516.
PROiQ8TDN6.

Gene expression databases

BgeeiQ8TDN6.
CleanExiHS_BXDC2.
GenevisibleiQ8TDN6. HS.

Family and domain databases

InterProiIPR004154. Anticodon-bd.
IPR007109. Brix.
IPR026532. BRX1.
[Graphical view]
PANTHERiPTHR13634. PTHR13634. 1 hit.
PfamiPF04427. Brix. 1 hit.
[Graphical view]
SMARTiSM00879. Brix. 1 hit.
[Graphical view]
SUPFAMiSSF52954. SSF52954. 1 hit.
PROSITEiPS50833. BRIX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Brix from Xenopus laevis and brx1p from yeast define a new family of proteins involved in the biogenesis of large ribosomal subunits."
    Kaser A., Bogengruber E., Hallegger M., Doppler E., Lepperdinger G., Jantsch M., Breitenbach M., Kreil G.
    Biol. Chem. 382:1637-1647(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "NovelFam3000 -- uncharacterized human protein domains conserved across model organisms."
    Kemmer D., Podowski R.M., Arenillas D., Lim J., Hodges E., Roth P., Sonnhammer E.L.L., Hoeoeg C., Wasserman W.W.
    BMC Genomics 7:48-48(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain cortex.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Cervix.
  6. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
    Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
    Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
    Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
    Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-276, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Uncovering global SUMOylation signaling networks in a site-specific manner."
    Hendriks I.A., D'Souza R.C., Yang B., Verlaan-de Vries M., Mann M., Vertegaal A.C.
    Nat. Struct. Mol. Biol. 21:927-936(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-322, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiBRX1_HUMAN
AccessioniPrimary (citable) accession number: Q8TDN6
Secondary accession number(s): A8K0P5
, Q3ZTT4, Q8N453, Q96DH1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 9, 2003
Last sequence update: May 9, 2003
Last modified: May 11, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.