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Protein

Potassium voltage-gated channel subfamily G member 4

Gene

KCNG4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Potassium channel subunit that does not form functional channels by itself. Can form functional heterotetrameric channels with KCNB1; modulates the delayed rectifier voltage-gated potassium channel activation and deactivation rates of KCNB1 (PubMed:19074135).1 Publication

GO - Molecular functioni

  • delayed rectifier potassium channel activity Source: UniProtKB
  • ion channel binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

ReactomeiREACT_75770. Voltage gated Potassium channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium voltage-gated channel subfamily G member 4
Alternative name(s):
Voltage-gated potassium channel subunit Kv6.4
Gene namesi
Name:KCNG4
Synonyms:KCNG3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:19697. KCNG4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 218218CytoplasmicBy similarityAdd
BLAST
Transmembranei219 – 24022Helical; Name=Segment S1By similarityAdd
BLAST
Topological domaini241 – 26121ExtracellularBy similarityAdd
BLAST
Transmembranei262 – 28322Helical; Name=Segment S2By similarityAdd
BLAST
Topological domaini284 – 29411CytoplasmicBy similarityAdd
BLAST
Transmembranei295 – 31420Helical; Name=Segment S3By similarityAdd
BLAST
Topological domaini315 – 32814ExtracellularBy similarityAdd
BLAST
Transmembranei329 – 35325Helical; Voltage-sensor; Name=Segment S4By similarityAdd
BLAST
Topological domaini354 – 36815CytoplasmicBy similarityAdd
BLAST
Transmembranei369 – 39022Helical; Name=Segment S5By similarityAdd
BLAST
Topological domaini391 – 40515ExtracellularBy similarityAdd
BLAST
Intramembranei406 – 41712Helical; Name=Pore helixBy similarityAdd
BLAST
Intramembranei418 – 4258By similarity
Topological domaini426 – 4327ExtracellularBy similarity
Transmembranei433 – 46129Helical; Name=Segment S6By similarityAdd
BLAST
Topological domaini462 – 51958CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134989953.

Polymorphism and mutation databases

BioMutaiKCNG4.
DMDMi26006803.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 519519Potassium voltage-gated channel subfamily G member 4PRO_0000054080Add
BLAST

Proteomic databases

PaxDbiQ8TDN1.
PRIDEiQ8TDN1.

PTM databases

PhosphoSiteiQ8TDN1.

Expressioni

Tissue specificityi

Highly expressed in brain, and at lower levels in liver, small intestine and colon.

Gene expression databases

BgeeiQ8TDN1.
CleanExiHS_KCNG3.
HS_KCNG4.
ExpressionAtlasiQ8TDN1. baseline and differential.
GenevisibleiQ8TDN1. HS.

Organism-specific databases

HPAiHPA039161.

Interactioni

Subunit structurei

Heterotetramer with KCNB1 (PubMed:19074135).1 Publication

Protein-protein interaction databases

BioGridi125002. 5 interactions.
STRINGi9606.ENSP00000312129.

Structurei

3D structure databases

ProteinModelPortaliQ8TDN1.
SMRiQ8TDN1. Positions 58-490.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi418 – 4236Selectivity filterBy similarity

Domaini

The transmembrane segment S4 functions as voltage-sensor and is characterized by a series of positively charged amino acids at every third position. Channel opening and closing is effected by a conformation change that affects the position and orientation of the voltage-sensor paddle formed by S3 and S4 within the membrane. A transmembrane electric field that is positive inside would push the positively charged S4 segment outwards, thereby opening the pore, while a field that is negative inside would pull the S4 segment inwards and close the pore. Changes in the position and orientation of S4 are then transmitted to the activation gate formed by the inner helix bundle via the S4-S5 linker region.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1226.
GeneTreeiENSGT00760000118981.
HOGENOMiHOG000231014.
HOVERGENiHBG100172.
InParanoidiQ8TDN1.
KOiK04903.
OMAiEMVENPQ.
OrthoDBiEOG7K9K2K.
PhylomeDBiQ8TDN1.
TreeFamiTF313103.

Family and domain databases

Gene3Di1.20.120.350. 1 hit.
InterProiIPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR003091. K_chnl.
IPR003968. K_chnl_volt-dep_Kv.
IPR003971. K_chnl_volt-dep_Kv9.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
[Graphical view]
PANTHERiPTHR11537. PTHR11537. 1 hit.
PfamiPF02214. BTB_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR00169. KCHANNEL.
PR01494. KV9CHANNEL.
PR01491. KVCHANNEL.
SMARTiSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8TDN1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPMPSRDGGL HPRHHHYGSH SPWSQLLSSP METPSIKGLY YRRVRKVGAL
60 70 80 90 100
DASPVDLKKE ILINVGGRRY LLPWSTLDRF PLSRLSKLRL CRSYEEIVQL
110 120 130 140 150
CDDYDEDSQE FFFDRSPSAF GVIVSFLAAG KLVLLQEMCA LSFQEELAYW
160 170 180 190 200
GIEEAHLERC CLRKLLRKLE ELEELAKLHR EDVLRQQRET RRPASHSSRW
210 220 230 240 250
GLCMNRLREM VENPQSGLPG KVFACLSILF VATTAVSLCV STMPDLRAEE
260 270 280 290 300
DQGECSRKCY YIFIVETICV AWFSLEFCLR FVQAQDKCQF FQGPLNIIDI
310 320 330 340 350
LAISPYYVSL AVSEEPPEDG ERPSGSSYLE KVGLVLRVLR ALRILYVMRL
360 370 380 390 400
ARHSLGLQTL GLTVRRCTRE FGLLLLFLAV AITLFSPLVY VAEKESGRVL
410 420 430 440 450
EFTSIPASYW WAIISMTTVG YGDMVPRSVP GQMVALSSIL SGILIMAFPA
460 470 480 490 500
TSIFHTFSHS YLELKKEQEQ LQARLRHLQN TGPASECELL DPHVASEHEL
510
MNDVNDLILE GPALPIMHM
Length:519
Mass (Da):58,979
Last modified:June 1, 2002 - v1
Checksum:iE5BBA354931AB0A4
GO
Isoform 2 (identifier: Q8TDN1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     253-256: GECS → VSGL
     257-519: Missing.

Note: No experimental confirmation available.
Show »
Length:256
Mass (Da):29,334
Checksum:i5A48EFC68637F8B8
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti8 – 81G → W.
Corresponds to variant rs35379218 [ dbSNP | Ensembl ].
VAR_053861
Natural varianti206 – 2061R → W.
Corresponds to variant rs11646443 [ dbSNP | Ensembl ].
VAR_053862
Natural varianti255 – 2551C → Y.
Corresponds to variant rs17736370 [ dbSNP | Ensembl ].
VAR_053863
Natural varianti321 – 3211E → K.
Corresponds to variant rs4782905 [ dbSNP | Ensembl ].
VAR_053864
Natural varianti325 – 3251G → R.
Corresponds to variant rs7196482 [ dbSNP | Ensembl ].
VAR_053865
Natural varianti427 – 4271R → H.
Corresponds to variant rs35649980 [ dbSNP | Ensembl ].
VAR_053866

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei253 – 2564GECS → VSGL in isoform 2. 1 PublicationVSP_001029
Alternative sequencei257 – 519263Missing in isoform 2. 1 PublicationVSP_001030Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF348984 mRNA. Translation: AAL83911.1.
BC008969 mRNA. Translation: AAH08969.1.
CCDSiCCDS10945.1. [Q8TDN1-1]
RefSeqiNP_758857.1. NM_172347.2. [Q8TDN1-1]
UniGeneiHs.335877.
Hs.560407.
Hs.737415.

Genome annotation databases

EnsembliENST00000308251; ENSP00000312129; ENSG00000168418.
ENST00000568181; ENSP00000457897; ENSG00000168418. [Q8TDN1-2]
GeneIDi93107.
KEGGihsa:93107.
UCSCiuc002fhu.1. human. [Q8TDN1-2]
uc010voc.2. human. [Q8TDN1-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF348984 mRNA. Translation: AAL83911.1.
BC008969 mRNA. Translation: AAH08969.1.
CCDSiCCDS10945.1. [Q8TDN1-1]
RefSeqiNP_758857.1. NM_172347.2. [Q8TDN1-1]
UniGeneiHs.335877.
Hs.560407.
Hs.737415.

3D structure databases

ProteinModelPortaliQ8TDN1.
SMRiQ8TDN1. Positions 58-490.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125002. 5 interactions.
STRINGi9606.ENSP00000312129.

Chemistry

ChEMBLiCHEMBL2362996.

PTM databases

PhosphoSiteiQ8TDN1.

Polymorphism and mutation databases

BioMutaiKCNG4.
DMDMi26006803.

Proteomic databases

PaxDbiQ8TDN1.
PRIDEiQ8TDN1.

Protocols and materials databases

DNASUi93107.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000308251; ENSP00000312129; ENSG00000168418.
ENST00000568181; ENSP00000457897; ENSG00000168418. [Q8TDN1-2]
GeneIDi93107.
KEGGihsa:93107.
UCSCiuc002fhu.1. human. [Q8TDN1-2]
uc010voc.2. human. [Q8TDN1-1]

Organism-specific databases

CTDi93107.
GeneCardsiGC16M084255.
HGNCiHGNC:19697. KCNG4.
HPAiHPA039161.
MIMi607603. gene.
neXtProtiNX_Q8TDN1.
PharmGKBiPA134989953.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG1226.
GeneTreeiENSGT00760000118981.
HOGENOMiHOG000231014.
HOVERGENiHBG100172.
InParanoidiQ8TDN1.
KOiK04903.
OMAiEMVENPQ.
OrthoDBiEOG7K9K2K.
PhylomeDBiQ8TDN1.
TreeFamiTF313103.

Enzyme and pathway databases

ReactomeiREACT_75770. Voltage gated Potassium channels.

Miscellaneous databases

GeneWikiiKCNG4.
GenomeRNAii93107.
NextBioi77984.
PROiQ8TDN1.
SOURCEiSearch...

Gene expression databases

BgeeiQ8TDN1.
CleanExiHS_KCNG3.
HS_KCNG4.
ExpressionAtlasiQ8TDN1. baseline and differential.
GenevisibleiQ8TDN1. HS.

Family and domain databases

Gene3Di1.20.120.350. 1 hit.
InterProiIPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR003091. K_chnl.
IPR003968. K_chnl_volt-dep_Kv.
IPR003971. K_chnl_volt-dep_Kv9.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
[Graphical view]
PANTHERiPTHR11537. PTHR11537. 1 hit.
PfamiPF02214. BTB_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR00169. KCHANNEL.
PR01494. KV9CHANNEL.
PR01491. KVCHANNEL.
SMARTiSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Obligatory heterotetramerization of three previously uncharacterized Kv channel alpha-subunits identified in the human genome."
    Ottschytsch N., Raes A., Van Hoorick D., Snyders D.J.
    Proc. Natl. Acad. Sci. U.S.A. 99:7986-7991(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Muscle.
  3. "Mutation of histidine 105 in the T1 domain of the potassium channel Kv2.1 disrupts heteromerization with Kv6.3 and Kv6.4."
    Mederos y Schnitzler M., Rinne S., Skrobek L., Renigunta V., Schlichthorl G., Derst C., Gudermann T., Daut J., Preisig-Muller R.
    J. Biol. Chem. 284:4695-4704(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, INTERACTION WITH KCNB1, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiKCNG4_HUMAN
AccessioniPrimary (citable) accession number: Q8TDN1
Secondary accession number(s): Q96H24
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2002
Last sequence update: June 1, 2002
Last modified: July 22, 2015
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.