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Protein

Neuropilin and tolloid-like protein 1

Gene

NETO1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the development and/or maintenance of neuronal circuitry. Accessory subunit of the neuronal N-methyl-D-aspartate receptor (NMDAR) critical for maintaining the abundance of GRIN2A-containing NMDARs in the postsynaptic density. Regulates long-term NMDA receptor-dependent synaptic plasticity and cognition, at least in the context of spatial learning and memory (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Receptor

Protein family/group databases

TCDBi8.A.47.1.1. the neuropilin and tolloid-like (neto) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Neuropilin and tolloid-like protein 1
Alternative name(s):
Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1
Gene namesi
Name:NETO1
Synonyms:BTCL1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 18

Organism-specific databases

HGNCiHGNC:13823. NETO1.

Subcellular locationi

Isoform 2 :

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini23 – 344322ExtracellularSequence analysisAdd
BLAST
Transmembranei345 – 36521HelicalSequence analysisAdd
BLAST
Topological domaini366 – 533168CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Secreted, Synapse

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA31558.

Polymorphism and mutation databases

BioMutaiNETO1.
DMDMi334302837.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence analysisAdd
BLAST
Chaini23 – 533511Neuropilin and tolloid-like protein 1PRO_0000021799Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi41 ↔ 68By similarity
Disulfide bondi96 ↔ 118By similarity
Disulfide bondi172 ↔ 202By similarity
Disulfide bondi229 ↔ 251By similarity
Disulfide bondi292 ↔ 304By similarity
Disulfide bondi299 ↔ 317By similarity
Glycosylationi306 – 3061N-linked (GlcNAc...)Sequence analysis
Disulfide bondi311 ↔ 326By similarity
Glycosylationi340 – 3401N-linked (GlcNAc...)Sequence analysis
Modified residuei417 – 4171PhosphotyrosineBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ8TDF5.
PaxDbiQ8TDF5.
PeptideAtlasiQ8TDF5.
PRIDEiQ8TDF5.

PTM databases

iPTMnetiQ8TDF5.
PhosphoSiteiQ8TDF5.

Expressioni

Tissue specificityi

Isoform 1 and isoform 2 are retina-specific. Isoform 3 is found in retina as well as at lower levels in adult and fetal brain.1 Publication

Gene expression databases

BgeeiENSG00000166342.
CleanExiHS_NETO1.
ExpressionAtlasiQ8TDF5. baseline and differential.
GenevisibleiQ8TDF5. HS.

Organism-specific databases

HPAiHPA064630.

Interactioni

Subunit structurei

Interacts with PLZ domains of DLG2, DLG3 and DLG4 via its C-terminal TRV domain. Interacts with GRIN2A and GRIN2B via its CUB domains (By similarity).By similarity

Protein-protein interaction databases

BioGridi123590. 1 interaction.
STRINGi9606.ENSP00000313088.

Structurei

3D structure databases

ProteinModelPortaliQ8TDF5.
SMRiQ8TDF5. Positions 40-269.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini41 – 155115CUB 1PROSITE-ProRule annotationAdd
BLAST
Domaini172 – 287116CUB 2PROSITE-ProRule annotationAdd
BLAST
Domaini291 – 32737LDL-receptor class APROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi531 – 5333PDZ-binding

Sequence similaritiesi

Contains 2 CUB domains.PROSITE-ProRule annotation
Contains 1 LDL-receptor class A domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IJXA. Eukaryota.
ENOG410ZS19. LUCA.
GeneTreeiENSGT00760000119018.
HOGENOMiHOG000060303.
HOVERGENiHBG052600.
InParanoidiQ8TDF5.
OMAiTEAVDCK.
OrthoDBiEOG091G060O.
PhylomeDBiQ8TDF5.

Family and domain databases

Gene3Di2.60.120.290. 2 hits.
4.10.400.10. 1 hit.
InterProiIPR000859. CUB_dom.
IPR023415. LDLR_class-A_CS.
IPR002172. LDrepeatLR_classA_rpt.
IPR028867. NETO1.
[Graphical view]
PANTHERiPTHR10127:SF315. PTHR10127:SF315. 1 hit.
PfamiPF00431. CUB. 2 hits.
PF00057. Ldl_recept_a. 1 hit.
[Graphical view]
SMARTiSM00042. CUB. 2 hits.
SM00192. LDLa. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 2 hits.
SSF57424. SSF57424. 1 hit.
PROSITEiPS01180. CUB. 2 hits.
PS01209. LDLRA_1. 1 hit.
PS50068. LDLRA_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 3 (identifier: Q8TDF5-3) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIHGRSVLHI VASLIILHLS GATKKGTEKQ TTSETQKSVQ CGTWTKHAEG
60 70 80 90 100
GIFTSPNYPS KYPPDRECIY IIEAAPRQCI ELYFDEKYSI EPSWECKFDH
110 120 130 140 150
IEVRDGPFGF SPIIGRFCGQ QNPPVIKSSG RFLWIKFFAD GELESMGFSA
160 170 180 190 200
RYNFTPDPDF KDLGALKPLP ACEFEMGGSE GIVESIQIMK EGKATASEAV
210 220 230 240 250
DCKWYIRAPP RSKIYLRFLD YEMQNSNECK RNFVAVYDGS SSVEDLKAKF
260 270 280 290 300
CSTVANDVML RTGLGVIRMW ADEGSRNSRF QMLFTSFQEP PCEGNTFFCH
310 320 330 340 350
SNMCINNTLV CNGLQNCVYP WDENHCKEKR KTSLLDQLTN TSGTVIGVTS
360 370 380 390 400
CIVIILIIIS VIVQIKQPRK KYVQRKSDFD QTVFQEVFEP PHYELCTLRG
410 420 430 440 450
TGATADFADV ADDFENYHKL RRSSSKCIHD HHCGSQLSST KGSRSNLSTR
460 470 480 490 500
DASILTEMPT QPGKPLIPPM NRRNILVMKH SYSQDAADAC DIDEIEEVPT
510 520 530
TSHRLSRHDK AVQRFCLIGS LSKHESEYNT TRV
Length:533
Mass (Da):60,191
Last modified:May 31, 2011 - v2
Checksum:i763EBE7BAF06EAC8
GO
Isoform 1 (identifier: Q8TDF5-1) [UniParc]FASTAAdd to basket
Also known as: Soluble, sNETO1

The sequence of this isoform differs from the canonical sequence as follows:
     1-10: MIHGRSVLHI → MLAEPFPKV
     157-157: D → E
     158-533: Missing.

Show »
Length:156
Mass (Da):17,669
Checksum:i25FE8BEF4553C2AB
GO
Isoform 2 (identifier: Q8TDF5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-10: MIHGRSVLHI → MLAEPFPKV

Show »
Length:532
Mass (Da):60,060
Checksum:iA4B0F5672D286E97
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti140 – 1401D → A in AAH50329 (PubMed:15489334).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti481 – 4811S → N.3 Publications
Corresponds to variant rs922999 [ dbSNP | Ensembl ].
VAR_051230
Natural varianti487 – 4871A → G.
Corresponds to variant rs17086286 [ dbSNP | Ensembl ].
VAR_051231

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1010MIHGRSVLHI → MLAEPFPKV in isoform 1 and isoform 2. 1 PublicationVSP_012853
Alternative sequencei157 – 1571D → E in isoform 1. 1 PublicationVSP_012854
Alternative sequencei158 – 533376Missing in isoform 1. 1 PublicationVSP_012855Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF448838 mRNA. Translation: AAM18026.1.
AF448839 mRNA. Translation: AAM18027.1.
AC023301 Genomic DNA. No translation available.
AC091138 Genomic DNA. No translation available.
BC050329 mRNA. Translation: AAH50329.1.
AL834354 mRNA. Translation: CAD39019.1.
CCDSiCCDS12000.1. [Q8TDF5-3]
CCDS42444.1. [Q8TDF5-1]
RefSeqiNP_001188394.1. NM_001201465.1.
NP_620416.1. NM_138966.3.
NP_620552.1. NM_138999.1. [Q8TDF5-1]
XP_005266831.1. XM_005266774.4. [Q8TDF5-3]
UniGeneiHs.465407.

Genome annotation databases

EnsembliENST00000327305; ENSP00000313088; ENSG00000166342. [Q8TDF5-3]
ENST00000397929; ENSP00000381024; ENSG00000166342. [Q8TDF5-1]
ENST00000583169; ENSP00000464312; ENSG00000166342. [Q8TDF5-3]
GeneIDi81832.
KEGGihsa:81832.
UCSCiuc002lkw.3. human. [Q8TDF5-3]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF448838 mRNA. Translation: AAM18026.1.
AF448839 mRNA. Translation: AAM18027.1.
AC023301 Genomic DNA. No translation available.
AC091138 Genomic DNA. No translation available.
BC050329 mRNA. Translation: AAH50329.1.
AL834354 mRNA. Translation: CAD39019.1.
CCDSiCCDS12000.1. [Q8TDF5-3]
CCDS42444.1. [Q8TDF5-1]
RefSeqiNP_001188394.1. NM_001201465.1.
NP_620416.1. NM_138966.3.
NP_620552.1. NM_138999.1. [Q8TDF5-1]
XP_005266831.1. XM_005266774.4. [Q8TDF5-3]
UniGeneiHs.465407.

3D structure databases

ProteinModelPortaliQ8TDF5.
SMRiQ8TDF5. Positions 40-269.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123590. 1 interaction.
STRINGi9606.ENSP00000313088.

Protein family/group databases

TCDBi8.A.47.1.1. the neuropilin and tolloid-like (neto) family.

PTM databases

iPTMnetiQ8TDF5.
PhosphoSiteiQ8TDF5.

Polymorphism and mutation databases

BioMutaiNETO1.
DMDMi334302837.

Proteomic databases

MaxQBiQ8TDF5.
PaxDbiQ8TDF5.
PeptideAtlasiQ8TDF5.
PRIDEiQ8TDF5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000327305; ENSP00000313088; ENSG00000166342. [Q8TDF5-3]
ENST00000397929; ENSP00000381024; ENSG00000166342. [Q8TDF5-1]
ENST00000583169; ENSP00000464312; ENSG00000166342. [Q8TDF5-3]
GeneIDi81832.
KEGGihsa:81832.
UCSCiuc002lkw.3. human. [Q8TDF5-3]

Organism-specific databases

CTDi81832.
GeneCardsiNETO1.
HGNCiHGNC:13823. NETO1.
HPAiHPA064630.
MIMi607973. gene.
neXtProtiNX_Q8TDF5.
PharmGKBiPA31558.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IJXA. Eukaryota.
ENOG410ZS19. LUCA.
GeneTreeiENSGT00760000119018.
HOGENOMiHOG000060303.
HOVERGENiHBG052600.
InParanoidiQ8TDF5.
OMAiTEAVDCK.
OrthoDBiEOG091G060O.
PhylomeDBiQ8TDF5.

Miscellaneous databases

GenomeRNAii81832.
PROiQ8TDF5.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000166342.
CleanExiHS_NETO1.
ExpressionAtlasiQ8TDF5. baseline and differential.
GenevisibleiQ8TDF5. HS.

Family and domain databases

Gene3Di2.60.120.290. 2 hits.
4.10.400.10. 1 hit.
InterProiIPR000859. CUB_dom.
IPR023415. LDLR_class-A_CS.
IPR002172. LDrepeatLR_classA_rpt.
IPR028867. NETO1.
[Graphical view]
PANTHERiPTHR10127:SF315. PTHR10127:SF315. 1 hit.
PfamiPF00431. CUB. 2 hits.
PF00057. Ldl_recept_a. 1 hit.
[Graphical view]
SMARTiSM00042. CUB. 2 hits.
SM00192. LDLa. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 2 hits.
SSF57424. SSF57424. 1 hit.
PROSITEiPS01180. CUB. 2 hits.
PS01209. LDLRA_1. 1 hit.
PS50068. LDLRA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNETO1_HUMAN
AccessioniPrimary (citable) accession number: Q8TDF5
Secondary accession number(s): Q86W85, Q8ND78, Q8TDF4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: May 31, 2011
Last modified: September 7, 2016
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.