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Protein

ATP-dependent RNA helicase DDX54

Gene

DDX54

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has RNA-dependent ATPase activity. Represses the transcriptional activity of nuclear receptors.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi140 – 147ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: UniProtKB
  • estrogen receptor binding Source: UniProtKB
  • receptor binding Source: UniProtKB
  • RNA binding Source: UniProtKB
  • transcription corepressor activity Source: UniProtKB

GO - Biological processi

  • intracellular estrogen receptor signaling pathway Source: UniProtKB
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • RNA metabolic process Source: UniProtKB
  • RNA processing Source: UniProtKB
  • RNA secondary structure unwinding Source: GO_Central
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionHelicase, Hydrolase, RNA-binding
Biological processTranscription, Transcription regulation
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase DDX54 (EC:3.6.4.13)
Alternative name(s):
ATP-dependent RNA helicase DP97
DEAD box RNA helicase 97 kDa
DEAD box protein 54
Gene namesi
Name:DDX54
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000123064.12.
HGNCiHGNC:20084. DDX54.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi79039.
OpenTargetsiENSG00000123064.
PharmGKBiPA134992026.

Polymorphism and mutation databases

BioMutaiDDX54.
DMDMi46576615.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000550561 – 881ATP-dependent RNA helicase DDX54Add BLAST881

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei34PhosphoserineCombined sources1
Modified residuei39PhosphoserineCombined sources1
Modified residuei41PhosphoserineCombined sources1
Modified residuei75PhosphoserineCombined sources1
Modified residuei644PhosphoserineCombined sources1
Modified residuei696PhosphoserineCombined sources1
Modified residuei698PhosphoserineCombined sources1
Modified residuei782PhosphoserineCombined sources1
Modified residuei788PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8TDD1.
MaxQBiQ8TDD1.
PaxDbiQ8TDD1.
PeptideAtlasiQ8TDD1.
PRIDEiQ8TDD1.

2D gel databases

SWISS-2DPAGEiQ8TDD1.

PTM databases

iPTMnetiQ8TDD1.
PhosphoSitePlusiQ8TDD1.
SwissPalmiQ8TDD1.

Expressioni

Gene expression databases

BgeeiENSG00000123064.
CleanExiHS_DDX54.
ExpressionAtlasiQ8TDD1. baseline and differential.
GenevisibleiQ8TDD1. HS.

Organism-specific databases

HPAiHPA028244.
HPA070786.

Interactioni

Subunit structurei

Interacts in a hormone-dependent manner with nuclear receptors.

GO - Molecular functioni

  • estrogen receptor binding Source: UniProtKB
  • receptor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi122503. 102 interactors.
IntActiQ8TDD1. 30 interactors.
MINTiMINT-3045390.
STRINGi9606.ENSP00000323858.

Structurei

3D structure databases

ProteinModelPortaliQ8TDD1.
SMRiQ8TDD1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini127 – 299Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST173
Domaini326 – 473Helicase C-terminalPROSITE-ProRule annotationAdd BLAST148

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni605 – 647Interaction with nuclear receptorsAdd BLAST43

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi96 – 124Q motifAdd BLAST29
Motifi247 – 250DEAD box4

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0337. Eukaryota.
ENOG410XQE5. LUCA.
GeneTreeiENSGT00550000075100.
HOGENOMiHOG000246455.
HOVERGENiHBG051333.
InParanoidiQ8TDD1.
KOiK14808.
OMAiLHLCVEM.
OrthoDBiEOG091G053F.
PhylomeDBiQ8TDD1.
TreeFamiTF105707.

Family and domain databases

CDDicd00079. HELICc. 1 hit.
InterProiView protein in InterPro
IPR012541. DBP10_C.
IPR033517. DDX54/DBP10.
IPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
PANTHERiPTHR24031:SF292. PTHR24031:SF292. 1 hit.
PfamiView protein in Pfam
PF08147. DBP10CT. 1 hit.
PF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD024971. DBP10CT. 1 hit.
SMARTiView protein in SMART
SM01123. DBP10CT. 1 hit.
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiView protein in PROSITE
PS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8TDD1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAADKGPAAG PRSRAAMAQW RKKKGLRKRR GAASQARGSD SEDGEFEIQA
60 70 80 90 100
EDDARARKLG PGRPLPTFPT SECTSDVEPD TREMVRAQNK KKKKSGGFQS
110 120 130 140 150
MGLSYPVFKG IMKKGYKVPT PIQRKTIPVI LDGKDVVAMA RTGSGKTACF
160 170 180 190 200
LLPMFERLKT HSAQTGARAL ILSPTRELAL QTLKFTKELG KFTGLKTALI
210 220 230 240 250
LGGDRMEDQF AALHENPDII IATPGRLVHV AVEMSLKLQS VEYVVFDEAD
260 270 280 290 300
RLFEMGFAEQ LQEIIARLPG GHQTVLFSAT LPKLLVEFAR AGLTEPVLIR
310 320 330 340 350
LDVDTKLNEQ LKTSFFLVRE DTKAAVLLHL LHNVVRPQDQ TVVFVATKHH
360 370 380 390 400
AEYLTELLTT QRVSCAHIYS ALDPTARKIN LAKFTLGKCS TLIVTDLAAR
410 420 430 440 450
GLDIPLLDNV INYSFPAKGK LFLHRVGRVA RAGRSGTAYS LVAPDEIPYL
460 470 480 490 500
LDLHLFLGRS LTLARPLKEP SGVAGVDGML GRVPQSVVDE EDSGLQSTLE
510 520 530 540 550
ASLELRGLAR VADNAQQQYV RSRPAPSPES IKRAKEMDLV GLGLHPLFSS
560 570 580 590 600
RFEEEELQRL RLVDSIKNYR SRATIFEINA SSRDLCSQVM RAKRQKDRKA
610 620 630 640 650
IARFQQGQQG RQEQQEGPVG PAPSRPALQE KQPEKEEEEE AGESVEDIFS
660 670 680 690 700
EVVGRKRQRS GPNRGAKRRR EEARQRDQEF YIPYRPKDFD SERGLSISGE
710 720 730 740 750
GGAFEQQAAG AVLDLMGDEA QNLTRGRQQL KWDRKKKRFV GQSGQEDKKK
760 770 780 790 800
IKTESGRYIS SSYKRDLYQK WKQKQKIDDR DSDEEGASDR RGPERRGGKR
810 820 830 840 850
DRGQGASRPH APGTPAGRVR PELKTKQQIL KQRRRAQKLH FLQRGGLKQL
860 870 880
SARNRRRVQE LQQGAFGRGA RSKKGKMRKR M
Length:881
Mass (Da):98,595
Last modified:April 26, 2004 - v2
Checksum:i9823C75156D998C4
GO
Isoform 2 (identifier: Q8TDD1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     804-804: Q → QA

Show »
Length:882
Mass (Da):98,666
Checksum:i947F50470F865F96
GO

Sequence cautioni

Q8TDD1: The sequence AAN59978 differs from that shown. Contaminating sequence. Sequence of unknown origin in the N-terminal part.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_052171570R → H. Corresponds to variant dbSNP:rs35519289Ensembl.1
Natural variantiVAR_052172693R → Q. Corresponds to variant dbSNP:rs11564Ensembl.1
Natural variantiVAR_052173712V → A. Corresponds to variant dbSNP:rs10354Ensembl.1
Natural variantiVAR_033860821P → L. Corresponds to variant dbSNP:rs1048889Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_040135804Q → QA in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF478457 mRNA. Translation: AAL85336.1.
AC089999 Genomic DNA. No translation available.
AY148094 mRNA. Translation: AAN59978.1. Sequence problems.
BC005848 mRNA. Translation: AAH05848.2.
CCDSiCCDS31907.1. [Q8TDD1-1]
CCDS44984.1. [Q8TDD1-2]
RefSeqiNP_001104792.1. NM_001111322.1. [Q8TDD1-2]
NP_076977.3. NM_024072.3. [Q8TDD1-1]
UniGeneiHs.506861.

Genome annotation databases

EnsembliENST00000306014; ENSP00000304072; ENSG00000123064. [Q8TDD1-1]
ENST00000314045; ENSP00000323858; ENSG00000123064. [Q8TDD1-2]
GeneIDi79039.
KEGGihsa:79039.
UCSCiuc001tup.4. human. [Q8TDD1-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiDDX54_HUMAN
AccessioniPrimary (citable) accession number: Q8TDD1
Secondary accession number(s): Q86YT8, Q9BRZ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: April 26, 2004
Last modified: October 25, 2017
This is version 151 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families