Q8TDD1 (DDX54_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent RNA helicase DDX54
EC=3.6.4.13

Alternative name(s):
ATP-dependent RNA helicase DP97
DEAD box RNA helicase 97 kDa
DEAD box protein 54

Gene names

Name:

DDX54

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	881 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has RNA-dependent ATPase activity. Represses the transcriptional activity of nuclear receptors. Ref.3
Catalytic activity	ATP + H2O = ADP + phosphate.
Subunit structure	Interacts in a hormone-dependent manner with nuclear receptors.
Subcellular location	Nucleus › nucleolus Ref.5.
Sequence similarities	Belongs to the DEAD box helicase family. DDX54/DBP10 subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
Sequence caution	The sequence AAN59978.1 differs from that shown. Reason: Contaminating sequence. Sequence of unknown origin in the N-terminal part.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Helicase Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA processing Inferred from direct assay Ref.3. Source: UniProtKB intracellular estrogen receptor signaling pathway Traceable author statement Ref.3. Source: UniProtKB regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleolus Inferred from direct assay Ref.3. Source: UniProtKB
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent RNA helicase activity Inferred from direct assay Ref.3. Source: UniProtKB RNA binding Inferred from electronic annotation. Source: UniProtKB-KW estrogen receptor binding Inferred from direct assay Ref.3. Source: UniProtKB transcription corepressor activity Inferred from direct assay Ref.3. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8TDD1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8TDD1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     804-804: Q → QA

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 881	881	ATP-dependent RNA helicase DDX54		PRO_0000055056
Regions
	Domain	127 – 299	173	Helicase ATP-binding
	Domain	326 – 473	148	Helicase C-terminal
	Nucleotide binding	140 – 147	8	ATP By similarity
	Region	605 – 647	43	Interaction with nuclear receptors
	Motif	96 – 124	29	Q motif
	Motif	247 – 250	4	DEAD box
Amino acid modifications
	Modified residue	34	1	Phosphoserine Ref.7 Ref.8
	Modified residue	39	1	Phosphoserine Ref.8 Ref.11
	Modified residue	41	1	Phosphoserine Ref.8 Ref.11
	Modified residue	75	1	Phosphoserine Ref.7
	Modified residue	644	1	Phosphoserine Ref.7
	Modified residue	696	1	Phosphoserine Ref.9
	Modified residue	698	1	Phosphoserine Ref.9
	Modified residue	782	1	Phosphoserine Ref.6 Ref.7 Ref.8 Ref.9 Ref.11
	Modified residue	788	1	Phosphoserine Ref.7
Natural variations
	Alternative sequence	804	1	Q → QA in isoform 2.		VSP_040135
	Natural variant	570	1	R → H. Corresponds to variant rs35519289 [ dbSNP | Ensembl ].		VAR_052171
	Natural variant	693	1	R → Q. Corresponds to variant rs11564 [ dbSNP | Ensembl ].		VAR_052172
	Natural variant	712	1	V → A. Corresponds to variant rs10354 [ dbSNP | Ensembl ].		VAR_052173
	Natural variant	821	1	P → L. Corresponds to variant rs1048889 [ dbSNP | Ensembl ].		VAR_033860

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 26, 2004. Version 2. Checksum: 9823C75156D998C4	FASTA	881	98,595
	10 20 30 40 50 60 MAADKGPAAG PRSRAAMAQW RKKKGLRKRR GAASQARGSD SEDGEFEIQA EDDARARKLG 70 80 90 100 110 120 PGRPLPTFPT SECTSDVEPD TREMVRAQNK KKKKSGGFQS MGLSYPVFKG IMKKGYKVPT 130 140 150 160 170 180 PIQRKTIPVI LDGKDVVAMA RTGSGKTACF LLPMFERLKT HSAQTGARAL ILSPTRELAL 190 200 210 220 230 240 QTLKFTKELG KFTGLKTALI LGGDRMEDQF AALHENPDII IATPGRLVHV AVEMSLKLQS 250 260 270 280 290 300 VEYVVFDEAD RLFEMGFAEQ LQEIIARLPG GHQTVLFSAT LPKLLVEFAR AGLTEPVLIR 310 320 330 340 350 360 LDVDTKLNEQ LKTSFFLVRE DTKAAVLLHL LHNVVRPQDQ TVVFVATKHH AEYLTELLTT 370 380 390 400 410 420 QRVSCAHIYS ALDPTARKIN LAKFTLGKCS TLIVTDLAAR GLDIPLLDNV INYSFPAKGK 430 440 450 460 470 480 LFLHRVGRVA RAGRSGTAYS LVAPDEIPYL LDLHLFLGRS LTLARPLKEP SGVAGVDGML 490 500 510 520 530 540 GRVPQSVVDE EDSGLQSTLE ASLELRGLAR VADNAQQQYV RSRPAPSPES IKRAKEMDLV 550 560 570 580 590 600 GLGLHPLFSS RFEEEELQRL RLVDSIKNYR SRATIFEINA SSRDLCSQVM RAKRQKDRKA 610 620 630 640 650 660 IARFQQGQQG RQEQQEGPVG PAPSRPALQE KQPEKEEEEE AGESVEDIFS EVVGRKRQRS 670 680 690 700 710 720 GPNRGAKRRR EEARQRDQEF YIPYRPKDFD SERGLSISGE GGAFEQQAAG AVLDLMGDEA 730 740 750 760 770 780 QNLTRGRQQL KWDRKKKRFV GQSGQEDKKK IKTESGRYIS SSYKRDLYQK WKQKQKIDDR 790 800 810 820 830 840 DSDEEGASDR RGPERRGGKR DRGQGASRPH APGTPAGRVR PELKTKQQIL KQRRRAQKLH 850 860 870 880 FLQRGGLKQL SARNRRRVQE LQQGAFGRGA RSKKGKMRKR M
	Isoform 2 [UniParc]. Checksum: 947F50470F865F96	FASTA	882	98,666
	10 20 30 40 50 60 MAADKGPAAG PRSRAAMAQW RKKKGLRKRR GAASQARGSD SEDGEFEIQA EDDARARKLG 70 80 90 100 110 120 PGRPLPTFPT SECTSDVEPD TREMVRAQNK KKKKSGGFQS MGLSYPVFKG IMKKGYKVPT 130 140 150 160 170 180 PIQRKTIPVI LDGKDVVAMA RTGSGKTACF LLPMFERLKT HSAQTGARAL ILSPTRELAL 190 200 210 220 230 240 QTLKFTKELG KFTGLKTALI LGGDRMEDQF AALHENPDII IATPGRLVHV AVEMSLKLQS 250 260 270 280 290 300 VEYVVFDEAD RLFEMGFAEQ LQEIIARLPG GHQTVLFSAT LPKLLVEFAR AGLTEPVLIR 310 320 330 340 350 360 LDVDTKLNEQ LKTSFFLVRE DTKAAVLLHL LHNVVRPQDQ TVVFVATKHH AEYLTELLTT 370 380 390 400 410 420 QRVSCAHIYS ALDPTARKIN LAKFTLGKCS TLIVTDLAAR GLDIPLLDNV INYSFPAKGK 430 440 450 460 470 480 LFLHRVGRVA RAGRSGTAYS LVAPDEIPYL LDLHLFLGRS LTLARPLKEP SGVAGVDGML 490 500 510 520 530 540 GRVPQSVVDE EDSGLQSTLE ASLELRGLAR VADNAQQQYV RSRPAPSPES IKRAKEMDLV 550 560 570 580 590 600 GLGLHPLFSS RFEEEELQRL RLVDSIKNYR SRATIFEINA SSRDLCSQVM RAKRQKDRKA 610 620 630 640 650 660 IARFQQGQQG RQEQQEGPVG PAPSRPALQE KQPEKEEEEE AGESVEDIFS EVVGRKRQRS 670 680 690 700 710 720 GPNRGAKRRR EEARQRDQEF YIPYRPKDFD SERGLSISGE GGAFEQQAAG AVLDLMGDEA 730 740 750 760 770 780 QNLTRGRQQL KWDRKKKRFV GQSGQEDKKK IKTESGRYIS SSYKRDLYQK WKQKQKIDDR 790 800 810 820 830 840 DSDEEGASDR RGPERRGGKR DRGQAGASRP HAPGTPAGRV RPELKTKQQI LKQRRRAQKL 850 860 870 880 HFLQRGGLKQ LSARNRRRVQ ELQQGAFGRG ARSKKGKMRK RM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Positional cloning of the Flv gene." Perelygin A.A. Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Placenta.
[2]	"The finished DNA sequence of human chromosome 12." Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R. , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A. Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Regulation of nuclear receptor transcriptional activity by a novel DEAD box RNA helicase (DP97)." Rajendran R.R., Nye A.C., Frasor J., Balsara R.D., Martini P.G., Katzenellenbogen B.S. J. Biol. Chem. 278:4628-4638(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-881 (ISOFORM 1), FUNCTION.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 18-881 (ISOFORM 1). Tissue: Kidney.
[5]	"Functional proteomic analysis of human nucleolus." Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C., Greco A., Hochstrasser D.F., Diaz J.-J. Mol. Biol. Cell 13:4100-4109(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[6]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-782, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-34; SER-75; SER-644; SER-782 AND SER-788, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-34; SER-39; SER-41 AND SER-782, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[9]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-696; SER-698 AND SER-782, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-39; SER-41 AND SER-782, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF478457 mRNA. Translation: AAL85336.1. AC089999 Genomic DNA. No translation available. AY148094 mRNA. Translation: AAN59978.1. Sequence problems. BC005848 mRNA. Translation: AAH05848.2.
IPI	IPI00152510. IPI00879999.
RefSeq	NP_001104792.1. NM_001111322.1. NP_076977.3. NM_024072.3.
UniGene	Hs.506861.
3D structure databases
ProteinModelPortal	Q8TDD1.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8TDD1. 5 interactions.
STRING	9606.ENSP00000323858.
PTM databases
PhosphoSite	Q8TDD1.
Polymorphism databases
DMDM	46576615.
2D gel databases
SWISS-2DPAGE	Q8TDD1.
Proteomic databases
PaxDb	Q8TDD1.
PRIDE	Q8TDD1.
Protocols and materials databases
DNASU	79039.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000306014; ENSP00000304072; ENSG00000123064. ENST00000314045; ENSP00000323858; ENSG00000123064.
GeneID	79039.
KEGG	hsa:79039.
UCSC	uc001tup.3. human. uc001tuq.4. human.
Organism-specific databases
CTD	79039.
GeneCards	GC12M113595.
HGNC	HGNC:20084. DDX54.
HPA	HPA028244.
MIM	611665. gene.
neXtProt	NX_Q8TDD1.
PharmGKB	PA134992026.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0513.
HOGENOM	HOG000246455.
HOVERGEN	HBG051333.
InParanoid	Q8TDD1.
KO	K14808.
OMA	FYIPYRP.
Gene expression databases
ArrayExpress	Q8TDD1.
Bgee	Q8TDD1.
CleanEx	HS_DDX54.
Genevestigator	Q8TDD1.
GermOnline	ENSG00000123064. Homo sapiens.
Family and domain databases
InterPro	IPR012541. DBP10CT. IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR014001. Helicase_ATP-bd. IPR001650. Helicase_C. IPR000629. RNA-helicase_DEAD-box_CS. IPR014014. RNA_helicase_DEAD_Q_motif. [Graphical view]
Pfam	PF08147. DBP10CT. 1 hit. PF00270. DEAD. 1 hit. PF00271. Helicase_C. 1 hit. [Graphical view]
ProDom	PD024971. DBP10CT. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS00039. DEAD_ATP_HELICASE. 1 hit. PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. PS51195. Q_MOTIF. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	DDX54. human.
GenomeRNAi	79039.
NextBio	67773.
SOURCE	Search...

Entry information

Entry name

DDX54_HUMAN

Accession

Primary (citable) accession number: Q8TDD1
Secondary accession number(s): Q86YT8, Q9BRZ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	April 26, 2004
Last modified:	May 1, 2013
This is version 107 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.