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Q8TD19

- NEK9_HUMAN

UniProt

Q8TD19 - NEK9_HUMAN

Protein

Serine/threonine-protein kinase Nek9

Gene

NEK9

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 140 (01 Oct 2014)
      Sequence version 2 (17 Oct 2006)
      Previous versions | rss
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    Functioni

    Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phase progression. Phosphorylates NEK6 and NEK7 and stimulates their activity by releasing the autoinhibitory functions of Tyr-108 and Tyr-97 respectively.3 Publications

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Cofactori

    Magnesium.

    Enzyme regulationi

    Activated during mitosis by intramolecular autophosphorylation. Activity and autophosphorylation is activated by manganese >> magnesium ions. Sensitive to increasing concentration of detergents. It is not cell-cycle regulated but activity is higher in G0-arrested cells.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei81 – 811ATP
    Active sitei176 – 1761Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi58 – 669ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. protein binding Source: IntAct
    4. protein kinase binding Source: UniProtKB
    5. protein serine/threonine kinase activity Source: Reactome

    GO - Biological processi

    1. mitotic cell cycle Source: Reactome
    2. mitotic nuclear division Source: UniProtKB-KW
    3. mitotic nuclear envelope disassembly Source: Reactome

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_160122. Activation of NIMA Kinases NEK9, NEK6, NEK7.
    REACT_163931. Nuclear Pore Complex (NPC) Disassembly.
    SignaLinkiQ8TD19.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein kinase Nek9 (EC:2.7.11.1)
    Alternative name(s):
    Nercc1 kinase
    Never in mitosis A-related kinase 9
    Short name:
    NimA-related protein kinase 9
    NimA-related kinase 8
    Short name:
    Nek8
    Gene namesi
    Name:NEK9
    Synonyms:KIAA1995, NEK8, NERCC
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 14

    Organism-specific databases

    HGNCiHGNC:18591. NEK9.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. cytosol Source: Reactome
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi81 – 811K → M: Loss of activity and autophosphorylation. 1 Publication
    Mutagenesisi210 – 2101T → A: Significant reduction of autophosphorylation. 1 Publication
    Mutagenesisi214 – 2141T → A: No effect on autophosphorylation. 1 Publication

    Organism-specific databases

    PharmGKBiPA38593.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 Publications
    Chaini2 – 979978Serine/threonine-protein kinase Nek9PRO_0000086435Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine2 Publications
    Modified residuei2 – 21Phosphoserine2 Publications
    Modified residuei13 – 131Phosphoserine1 Publication
    Modified residuei16 – 161Phosphoserine1 Publication
    Modified residuei52 – 521Phosphotyrosine1 Publication
    Modified residuei76 – 761Phosphoserine2 Publications
    Modified residuei210 – 2101Phosphothreonine; by autocatalysis1 Publication
    Modified residuei254 – 2541Phosphothreonine2 Publications
    Modified residuei333 – 3331Phosphothreonine2 Publications
    Modified residuei741 – 7411Phosphoserine1 Publication
    Modified residuei801 – 8011Phosphoserine2 Publications
    Modified residuei832 – 8321Phosphoserine1 Publication
    Modified residuei886 – 8861Phosphothreonine2 Publications
    Modified residuei944 – 9441Phosphoserine2 Publications
    Modified residuei978 – 9781Phosphoserine1 Publication

    Post-translational modificationi

    Autophosphorylated on serine and threonine residues. When complexed with FACT, exhibits markedly elevated phosphorylation on Thr-210. During mitosis, not phosphorylated on Thr-210. Phosphorylated by CDK1 in vitro.5 Publications

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ8TD19.
    PaxDbiQ8TD19.
    PeptideAtlasiQ8TD19.
    PRIDEiQ8TD19.

    PTM databases

    PhosphoSiteiQ8TD19.

    Expressioni

    Tissue specificityi

    Most abundant in heart, liver, kidney and testis. Also expressed in smooth muscle cells and fibroblasts.

    Developmental stagei

    Expression varied mildly across the cell cycle, with highest expression observed in G1 and stationary-phase cells.

    Gene expression databases

    ArrayExpressiQ8TD19.
    BgeeiQ8TD19.
    CleanExiHS_NEK8.
    HS_NEK9.
    GenevestigatoriQ8TD19.

    Organism-specific databases

    HPAiHPA001405.

    Interactioni

    Subunit structurei

    Homodimer. Binds to Ran GTPase. Has a greater affinity for Ran-GDP over Ran-GTP. Interacts with NEK6, NEK7 and BICD2. Interacts with SSRP1 and SUPT16H, the 2 subunits of the FACT complex. Interacts with DYNLL1; phosphorylation at Ser-944 strongly reduces DYNLL1 binding.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    GABARAPO951665EBI-1044009,EBI-712001
    GABARAPL1Q9H0R86EBI-1044009,EBI-746969
    GABARAPL2P605207EBI-1044009,EBI-720116
    HSP90AB1P082382EBI-1044009,EBI-352572
    MAP1LC3BQ9GZQ82EBI-1044009,EBI-373144
    MAP1LC3CQ9BXW42EBI-1044009,EBI-2603996
    PLK1P533505EBI-1044009,EBI-476768

    Protein-protein interaction databases

    BioGridi124876. 19 interactions.
    IntActiQ8TD19. 18 interactions.
    MINTiMINT-2984282.
    STRINGi9606.ENSP00000238616.

    Structurei

    Secondary structure

    1
    979
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi942 – 9487

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3ZKEX-ray2.20B/D/F/H/J/L940-950[»]
    3ZKFX-ray2.60B/D/F/H/J/L940-950[»]
    ProteinModelPortaliQ8TD19.
    SMRiQ8TD19. Positions 64-311, 342-751.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini52 – 308257Protein kinasePROSITE-ProRule annotationAdd
    BLAST
    Repeati388 – 44457RCC1 1Add
    BLAST
    Repeati445 – 49854RCC1 2Add
    BLAST
    Repeati499 – 55052RCC1 3Add
    BLAST
    Repeati551 – 61565RCC1 4Add
    BLAST
    Repeati616 – 66853RCC1 5Add
    BLAST
    Repeati669 – 72658RCC1 6Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni732 – 891160Interaction with NEK6Add
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili892 – 93948Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi752 – 7609Poly-Gly
    Compositional biasi765 – 888124Pro/Ser/Thr-richAdd
    BLAST

    Domaini

    Dimerizes through its coiled-coil domain.

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation
    Contains 6 RCC1 repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Repeat

    Phylogenomic databases

    eggNOGiCOG0515.
    HOVERGENiHBG039572.
    InParanoidiQ8TD19.
    KOiK08857.
    OMAiGCDGTFL.
    OrthoDBiEOG7G7KNB.
    PhylomeDBiQ8TD19.
    TreeFamiTF106472.

    Family and domain databases

    Gene3Di2.130.10.30. 1 hit.
    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR009091. RCC1/BLIP-II.
    IPR000408. Reg_chr_condens.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    PF00415. RCC1. 4 hits.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50985. SSF50985. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS50012. RCC1_3. 6 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8TD19-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSVLGEYERH CDSINSDFGS ESGGCGDSSP GPSASQGPRA GGGAAEQEEL    50
    HYIPIRVLGR GAFGEATLYR RTEDDSLVVW KEVDLTRLSE KERRDALNEI 100
    VILALLQHDN IIAYYNHFMD NTTLLIELEY CNGGNLYDKI LRQKDKLFEE 150
    EMVVWYLFQI VSAVSCIHKA GILHRDIKTL NIFLTKANLI KLGDYGLAKK 200
    LNSEYSMAET LVGTPYYMSP ELCQGVKYNF KSDIWAVGCV IFELLTLKRT 250
    FDATNPLNLC VKIVQGIRAM EVDSSQYSLE LIQMVHSCLD QDPEQRPTAD 300
    ELLDRPLLRK RRREMEEKVT LLNAPTKRPR SSTVTEAPIA VVTSRTSEVY 350
    VWGGGKSTPQ KLDVIKSGCS ARQVCAGNTH FAVVTVEKEL YTWVNMQGGT 400
    KLHGQLGHGD KASYRQPKHV EKLQGKAIRQ VSCGDDFTVC VTDEGQLYAF 450
    GSDYYGCMGV DKVAGPEVLE PMQLNFFLSN PVEQVSCGDN HVVVLTRNKE 500
    VYSWGCGEYG RLGLDSEEDY YTPQKVDVPK ALIIVAVQCG CDGTFLLTQS 550
    GKVLACGLNE FNKLGLNQCM SGIINHEAYH EVPYTTSFTL AKQLSFYKIR 600
    TIAPGKTHTA AIDERGRLLT FGCNKCGQLG VGNYKKRLGI NLLGGPLGGK 650
    QVIRVSCGDE FTIAATDDNH IFAWGNGGNG RLAMTPTERP HGSDICTSWP 700
    RPIFGSLHHV PDLSCRGWHT ILIVEKVLNS KTIRSNSSGL SIGTVFQSSS 750
    PGGGGGGGGG EEEDSQQESE TPDPSGGFRG TMEADRGMEG LISPTEAMGN 800
    SNGASSSCPG WLRKELENAE FIPMPDSPSP LSAAFSESEK DTLPYEELQG 850
    LKVASEAPLE HKPQVEASSP RLNPAVTCAG KGTPLTPPAC ACSSLQVEVE 900
    RLQGLVLKCL AEQQKLQQEN LQIFTQLQKL NKKLEGGQQV GMHSKGTQTA 950
    KEEMEMDPKP DLDSDSWCLL GTDSCRPSL 979
    Length:979
    Mass (Da):107,168
    Last modified:October 17, 2006 - v2
    Checksum:i8583FDDE599324A2
    GO

    Sequence cautioni

    The sequence BAC02704.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
    The sequence AAD31936.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti351 – 3511V → I in AAL87410. (PubMed:12101123)Curated
    Sequence conflicti967 – 9671W → G in AAL87410. (PubMed:12101123)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti429 – 4291R → H.3 Publications
    Corresponds to variant rs10146482 [ dbSNP | Ensembl ].
    VAR_027900
    Natural varianti828 – 8281P → T.1 Publication
    Corresponds to variant rs36014869 [ dbSNP | Ensembl ].
    VAR_040926
    Natural varianti870 – 8701P → S in a lung neuroendocrine carcinoma sample; somatic mutation. 1 Publication
    VAR_040927

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY048580 mRNA. Translation: AAL05428.1.
    AY080896 mRNA. Translation: AAL87410.1.
    AB082526 mRNA. Translation: BAC02704.1. Different initiation.
    AC007055 Genomic DNA. Translation: AAD31936.1. Sequence problems.
    AC007055 Genomic DNA. Translation: AAD31938.1.
    AC007055 Genomic DNA. Translation: AAD31939.1.
    AC007055 Genomic DNA. Translation: AAD31940.1.
    BC009336 mRNA. Translation: AAH09336.2.
    BC093881 mRNA. Translation: AAH93881.1.
    BC112101 mRNA. Translation: AAI12102.1.
    CCDSiCCDS9839.1.
    RefSeqiNP_149107.4. NM_033116.4.
    UniGeneiHs.7200.

    Genome annotation databases

    EnsembliENST00000238616; ENSP00000238616; ENSG00000119638.
    GeneIDi91754.
    KEGGihsa:91754.
    UCSCiuc001xrl.3. human.

    Polymorphism databases

    DMDMi116242675.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY048580 mRNA. Translation: AAL05428.1 .
    AY080896 mRNA. Translation: AAL87410.1 .
    AB082526 mRNA. Translation: BAC02704.1 . Different initiation.
    AC007055 Genomic DNA. Translation: AAD31936.1 . Sequence problems.
    AC007055 Genomic DNA. Translation: AAD31938.1 .
    AC007055 Genomic DNA. Translation: AAD31939.1 .
    AC007055 Genomic DNA. Translation: AAD31940.1 .
    BC009336 mRNA. Translation: AAH09336.2 .
    BC093881 mRNA. Translation: AAH93881.1 .
    BC112101 mRNA. Translation: AAI12102.1 .
    CCDSi CCDS9839.1.
    RefSeqi NP_149107.4. NM_033116.4.
    UniGenei Hs.7200.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3ZKE X-ray 2.20 B/D/F/H/J/L 940-950 [» ]
    3ZKF X-ray 2.60 B/D/F/H/J/L 940-950 [» ]
    ProteinModelPortali Q8TD19.
    SMRi Q8TD19. Positions 64-311, 342-751.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 124876. 19 interactions.
    IntActi Q8TD19. 18 interactions.
    MINTi MINT-2984282.
    STRINGi 9606.ENSP00000238616.

    Chemistry

    BindingDBi Q8TD19.
    ChEMBLi CHEMBL5257.
    GuidetoPHARMACOLOGYi 2124.

    PTM databases

    PhosphoSitei Q8TD19.

    Polymorphism databases

    DMDMi 116242675.

    Proteomic databases

    MaxQBi Q8TD19.
    PaxDbi Q8TD19.
    PeptideAtlasi Q8TD19.
    PRIDEi Q8TD19.

    Protocols and materials databases

    DNASUi 91754.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000238616 ; ENSP00000238616 ; ENSG00000119638 .
    GeneIDi 91754.
    KEGGi hsa:91754.
    UCSCi uc001xrl.3. human.

    Organism-specific databases

    CTDi 91754.
    GeneCardsi GC14M075548.
    HGNCi HGNC:18591. NEK9.
    HPAi HPA001405.
    MIMi 609798. gene.
    neXtProti NX_Q8TD19.
    PharmGKBi PA38593.
    HUGEi Search...
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0515.
    HOVERGENi HBG039572.
    InParanoidi Q8TD19.
    KOi K08857.
    OMAi GCDGTFL.
    OrthoDBi EOG7G7KNB.
    PhylomeDBi Q8TD19.
    TreeFami TF106472.

    Enzyme and pathway databases

    Reactomei REACT_160122. Activation of NIMA Kinases NEK9, NEK6, NEK7.
    REACT_163931. Nuclear Pore Complex (NPC) Disassembly.
    SignaLinki Q8TD19.

    Miscellaneous databases

    GeneWikii NEK9.
    GenomeRNAii 91754.
    NextBioi 77443.
    PROi Q8TD19.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8TD19.
    Bgeei Q8TD19.
    CleanExi HS_NEK8.
    HS_NEK9.
    Genevestigatori Q8TD19.

    Family and domain databases

    Gene3Di 2.130.10.30. 1 hit.
    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR009091. RCC1/BLIP-II.
    IPR000408. Reg_chr_condens.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    PF00415. RCC1. 4 hits.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50985. SSF50985. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS50012. RCC1_3. 6 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Purification, cloning, and characterization of Nek8, a novel NIMA-related kinase, and its candidate substrate Bicd2."
      Holland P.M., Milne A., Garka K., Johnson R.S., Willis C., Sims J.E., Rauch C.T., Bird T.A., Virca G.D.
      J. Biol. Chem. 277:16229-16240(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, PROTEIN SEQUENCE OF 61-70; 318-327; 331-352 AND 511-530, MUTAGENESIS OF THR-210 AND THR-214, VARIANT HIS-429.
      Tissue: Dendritic cell and Fibroblast.
    2. "Nercc1, a mammalian NIMA-family kinase, binds the Ran GTPase and regulates mitotic progression."
      Roig J., Mikhailov A., Belham C., Avruch J.
      Genes Dev. 16:1640-1658(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, PARTIAL PROTEIN SEQUENCE, MUTAGENESIS OF LYS-81.
    3. "Characterization of size-fractionated cDNA libraries generated by the in vitro recombination-assisted method."
      Ohara O., Nagase T., Mitsui G., Kohga H., Kikuno R., Hiraoka S., Takahashi Y., Kitajima S., Saga Y., Koseki H.
      DNA Res. 9:47-57(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT HIS-429.
      Tissue: Brain.
    4. "The DNA sequence and analysis of human chromosome 14."
      Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H.
      , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.
      Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    6. "A mitotic cascade of NIMA family kinases. Nercc1/Nek9 activates the Nek6 and Nek7 kinases."
      Belham C., Roig J., Caldwell J.A., Aoyama Y., Kemp B.E., Comb M., Avruch J.
      J. Biol. Chem. 278:34897-34909(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Nek9, a novel FACT-associated protein, modulates interphase progression."
      Tan B.C.-M., Lee S.-C.
      J. Biol. Chem. 279:9321-9330(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SSRP1 AND SUPT16H, SUBCELLULAR LOCATION, PHOSPHORYLATION AT THR-210.
    8. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
      Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
      Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    9. "The NIMA-family kinase Nek6 phosphorylates the kinesin Eg5 at a novel site necessary for mitotic spindle formation."
      Rapley J., Nicolas M., Groen A., Regue L., Bertran M.T., Caelles C., Avruch J., Roig J.
      J. Cell Sci. 121:3912-3921(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NEK6.
    10. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
      Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
      Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2; SER-76; THR-254; SER-741; SER-801; THR-886; SER-944 AND SER-978, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    11. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-333, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    12. "An autoinhibitory tyrosine motif in the cell-cycle-regulated Nek7 kinase is released through binding of Nek9."
      Richards M.W., O'Regan L., Mas-Droux C., Blot J.M., Cheung J., Hoelder S., Fry A.M., Bayliss R.
      Mol. Cell 36:560-570(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    13. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2; SER-13; SER-16; TYR-52; SER-76; THR-254; SER-801; SER-832; THR-886 AND SER-944, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    14. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-333, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.
    15. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    16. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    17. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
      Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
      Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    18. "Structural analysis of the regulation of the DYNLL/LC8 binding to Nek9 by phosphorylation."
      Gallego P., Velazquez-Campoy A., Regue L., Roig J., Reverter D.
      J. Biol. Chem. 288:12283-12294(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 940-950 IN COMPLEXES WITH DYNLL1, INTERACTION WITH DYNLL1.
    19. "Patterns of somatic mutation in human cancer genomes."
      Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G.
      , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
      Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANTS [LARGE SCALE ANALYSIS] HIS-429; THR-828 AND SER-870.

    Entry informationi

    Entry nameiNEK9_HUMAN
    AccessioniPrimary (citable) accession number: Q8TD19
    Secondary accession number(s): Q52LK6
    , Q8NCN0, Q8TCY4, Q9UPI4, Q9Y6S4, Q9Y6S5, Q9Y6S6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 15, 2003
    Last sequence update: October 17, 2006
    Last modified: October 1, 2014
    This is version 140 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 14
      Human chromosome 14: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    7. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3