Q8TCW9 (PKR1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Prokineticin receptor 1
Short name=PK-R1

Alternative name(s):
G-protein coupled receptor 73
G-protein coupled receptor ZAQ
GPR73a

Gene names

Name:	PROKR1
Synonyms:	GPR73, PKR1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for prokineticin 1. Exclusively coupled to the G(q) subclass of heteromeric G proteins. Activation leads to mobilization of calcium, stimulation of phosphoinositide turnover and activation of p44/p42 mitogen-activated protein kinase.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Expressed in the stomach, throughout the small intestine, colon, rectum, thyroid gland, pituitary gland, salivary gland, adrenal gland, testis, ovary, brain, spleen, prostate and pancreas.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of apoptotic process Inferred from electronic annotation. Source: Compara
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Traceable author statement. Source: Reactome
Molecular_function	neuropeptide Y receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 393	393	Prokineticin receptor 1		PRO_0000070079
Regions
	Topological domain	1 – 62	62	Extracellular Potential
	Transmembrane	63 – 83	21	Helical; Name=1; Potential
	Topological domain	84 – 98	15	Cytoplasmic Potential
	Transmembrane	99 – 119	21	Helical; Name=2; Potential
	Topological domain	120 – 146	27	Extracellular Potential
	Transmembrane	147 – 167	21	Helical; Name=3; Potential
	Topological domain	168 – 180	13	Cytoplasmic Potential
	Transmembrane	181 – 201	21	Helical; Name=4; Potential
	Topological domain	202 – 232	31	Extracellular Potential
	Transmembrane	233 – 253	21	Helical; Name=5; Potential
	Topological domain	254 – 282	29	Cytoplasmic Potential
	Transmembrane	283 – 303	21	Helical; Name=6; Potential
	Topological domain	304 – 322	19	Extracellular Potential
	Transmembrane	323 – 343	21	Helical; Name=7; Potential
	Topological domain	344 – 393	50	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	11	1	N-linked (GlcNAc...) Potential
	Glycosylation	14	1	N-linked (GlcNAc...) Potential
	Glycosylation	36	1	N-linked (GlcNAc...) Potential
	Disulfide bond	137 ↔ 217		By similarity
Natural variations
	Natural variant	40	1	S → G. Corresponds to variant rs7570797 [ dbSNP | Ensembl ].		VAR_024261
Experimental info
	Sequence conflict	255 – 256	2	IS → MT in AAM48127. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q8TCW9 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 0D36461CA99CAEC1	FASTA	393	44,770
	10 20 30 40 50 60 METTMGFMDD NATNTSTSFL SVLNPHGAHA TSFPFNFSYS DYDMPLDEDE DVTNSRTFFA 70 80 90 100 110 120 AKIVIGMALV GIMLVCGIGN FIFIAALVRY KKLRNLTNLL IANLAISDFL VAIVCCPFEM 130 140 150 160 170 180 DYYVVRQLSW EHGHVLCTSV NYLRTVSLYV STNALLAIAI DRYLAIVHPL RPRMKCQTAT 190 200 210 220 230 240 GLIALVWTVS ILIAIPSAYF TTETVLVIVK SQEKIFCGQI WPVDQQLYYK SYFLFIFGIE 250 260 270 280 290 300 FVGPVVTMTL CYARISRELW FKAVPGFQTE QIRKRLRCRR KTVLVLMCIL TAYVLCWAPF 310 320 330 340 350 360 YGFTIVRDFF PTVFVKEKHY LTAFYIVECI AMSNSMINTL CFVTVKNDTV KYFKKIMLLH 370 380 390 WKASYNGGKS SADLDLKTIG MPATEEVDCI RLK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and identification of EG-VEGF/prokineticins as cognate ligands for two orphan G-protein-coupled receptors." Masuda Y., Takatsu Y., Terao Y., Kumano S., Ishibashi Y., Suenaga M., Abe M., Fukusumi S., Watanabe T., Shintani Y., Yamada T., Hinuma S., Inatomi N., Ohtaki T., Onda H., Fujino M. Biochem. Biophys. Res. Commun. 293:396-402(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Molecular cloning and characterization of prokineticin receptors." Soga T., Matsumoto S., Oda T., Saito T., Hiyama H., Takasaki J., Kamohara M., Ohishi T., Matsushime H., Furuichi K. Biochim. Biophys. Acta 1579:173-179(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Identification and molecular characterization of two closely related G protein-coupled receptors activated by prokineticins/endocrine gland vascular endothelial growth factor." Lin D.C.-H., Bullock C.M., Ehlert F.J., Chen J.-L., Tian H., Zhou Q.-Y. J. Biol. Chem. 277:19276-19280(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Martin A.L., Kaighin V.A., Aronstam R.S. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY089976 mRNA. Translation: AAM11892.1. AB084080 mRNA. Translation: BAC24021.1. AF506287 mRNA. Translation: AAM48127.1. AC105054 Genomic DNA. Translation: AAY24114.1. EF577399 mRNA. Translation: ABQ52419.1. BC100960 mRNA. Translation: AAI00961.1.
IPI	IPI00152470.
RefSeq	NP_620414.1. NM_138964.2.
UniGene	Hs.683430.
3D structure databases
ProteinModelPortal	Q8TCW9.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000303775.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	Q8TCW9.
Polymorphism databases
DMDM	33112428.
Proteomic databases
PaxDb	Q8TCW9.
PRIDE	Q8TCW9.
Protocols and materials databases
DNASU	10887.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000303786; ENSP00000303775; ENSG00000169618. ENST00000394342; ENSP00000377874; ENSG00000169618.
GeneID	10887.
KEGG	hsa:10887.
UCSC	uc010yqj.2. human.
Organism-specific databases
CTD	10887.
GeneCards	GC02P068870.
HGNC	HGNC:4524. PROKR1.
HPA	HPA029396.
MIM	607122. gene.
neXtProt	NX_Q8TCW9.
PharmGKB	PA28916.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG319668.
HOGENOM	HOG000231664.
HOVERGEN	HBG039631.
InParanoid	Q8TCW9.
KO	K08379.
OMA	LIIVKSQ.
OrthoDB	EOG4SF966.
PhylomeDB	Q8TCW9.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
Bgee	Q8TCW9.
CleanEx	HS_PROKR1.
Genevestigator	Q8TCW9.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR000611. NPY_rcpt. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR01012. NRPEPTIDEYR.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q8TCW9.
ChEMBL	CHEMBL5649.
GenomeRNAi	10887.
NextBio	41341.
SOURCE	Search...

Entry information

Entry name

PKR1_HUMAN

Accession

Primary (citable) accession number: Q8TCW9
Secondary accession number(s): A5JUU2, Q53QT9, Q8NFJ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2003
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.