Reviewed, UniProtKB/Swiss-Prot Q8TCV5 (WFDC5_HUMAN)

Names and origin

Protein names

Recommended name:
    WAP four-disulfide core domain protein 5
Alternative name(s):
    Putative protease inhibitor WAP1
    p53-responsive gene 5 protein

Gene names

Name:	WFDC5
Synonyms:	PRG5, WAP1
ORF Names:	UNQ3036/PRO9842

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	224 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Putative acid-stable proteinase inhibitor.
Subcellular location	Secreted Potential.
Induction	By p53/TP53. Ref.5
Sequence similarities	Contains 2 WAP domains.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat Signal
Molecular function	Protease inhibitor Serine protease inhibitor
PTM	Disulfide bond
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8TCV5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Gene prediction confirmed by EST data.

Isoform 2 (identifier: Q8TCV5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     124-224: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 224	200	WAP four-disulfide core domain protein 5		PRO_0000041382
Regions
	Domain	27 – 73	47	WAP 1
	Domain	74 – 121	48	WAP 2
Amino acid modifications
	Disulfide bond	34 ↔ 62		By similarity
	Disulfide bond	41 ↔ 66		By similarity
	Disulfide bond	49 ↔ 61		By similarity
	Disulfide bond	55 ↔ 70		By similarity
	Disulfide bond	81 ↔ 109		By similarity
	Disulfide bond	88 ↔ 113		By similarity
	Disulfide bond	96 ↔ 108		By similarity
	Disulfide bond	102 ↔ 117		By similarity
Natural variations
	Alternative sequence	124 – 224	101	Missing in isoform 2.		VSP_006756
	Natural variant	97	1	H → Y: dbSNP rs17422688.		VAR_057474
Experimental info
	Sequence conflict	172	1	Q → R in AF147078. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 305CC59024058F15	FASTA	224	24,238
	10 20 30 40 50 60 MRTQSLLLLG ALLAVGSQLP AVFGRKKGEK SGGCPPDDGP CLLSVPDQCV EDSQCPLTRK 70 80 90 100 110 120 CCYRACFRQC VPRVSVKLGS CPEDQLRCLS PMNHLCHKDS DCSGKKRCCH SACGRDCRDP 130 140 150 160 170 180 ARGTAPGCPG QANSDLGSVA LHLSWGPTER VHDGRPGALP AGQHYLYQRW FQPSDNHWPA 190 200 210 220 DTSLQPIHPW FLLLGVKVHS LSSEEGLCIT PVLCTTAIRA SHPS
	Isoform 2. Checksum: 255B5501B24F54F4	FASTA	123	13,313
	10 20 30 40 50 60 MRTQSLLLLG ALLAVGSQLP AVFGRKKGEK SGGCPPDDGP CLLSVPDQCV EDSQCPLTRK 70 80 90 100 110 120 CCYRACFRQC VPRVSVKLGS CPEDQLRCLS PMNHLCHKDS DCSGKKRCCH SACGRDCRDP ARG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A locus on human chromosome 20 contains several genes expressing protease inhibitor domains with homology to whey acidic protein." Clauss A., Lilja H., Lundwall A. Biochem. J. 368:233-242(2002) [PubMed: 12206714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT TYR-97.
[3]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed: 11780052] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Skin.
[5]	"Isolation of differentially expressed cDNAs from p53-dependent apoptotic cells: activation of the human homologue of the Drosophila peroxidasin gene." Horikoshi N., Cong J., Kley N., Shenk T. Biochem. Biophys. Res. Commun. 261:864-869(1999) [PubMed: 10441517] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 145-224 (ISOFORM 1), INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY038181 mRNA. Translation: AAK72468.1. AY358822 mRNA. Translation: AAQ89181.1. Z93016 Genomic DNA. Translation: CAD27771.1. Z93016 Genomic DNA. Translation: CAI19816.1. BC039173 mRNA. Translation: AAH39173.1. AF147078 mRNA. No translation available.
IPI	IPI00152461. IPI00221015.
RefSeq	NP_663627.1.
UniGene	Hs.375031
3D structure databases
HSSP	HSSP built from PDB template 2REL based on UniProtKB P19957.
ModBase	Search...
Proteomic databases
PRIDE	Q8TCV5.
Genome annotation databases
Ensembl	ENSG00000175121. Homo sapiens. [Contig view]
GeneID	149708.
KEGG	hsa:149708.
Organism-specific databases
GeneCards	GC20M043171.
HGNC	HGNC:20477. WFDC5.
MIM	605161. gene.
PharmGKB	PA134885667.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q8TCV5.
HOVERGEN	Q8TCV5.
OMA	Q8TCV5. FLLLGVK.
Gene expression databases
ArrayExpress	Q8TCV5.
Bgee	Q8TCV5.
CleanEx	HS_WFDC5.
GermOnline	ENSG00000175121. Homo sapiens.
Family and domain databases
InterPro	IPR015874. 4-disulphide_core. IPR018069. Whey_acidic_4-diS_core_CS. IPR008197. Whey_acidic_protein_4-diS_core. [Graphical view]
Gene3D	G3DSA:4.10.75.10. Whey_acidic_protein_4-diS_core. 2 hits.
Pfam	PF00095. WAP. 2 hits. [Graphical view]
PRINTS	PR00003. 4DISULPHCORE.
ProDom	PD001224. Prot_inh_I17. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00217. WAP. 2 hits. [Graphical view]
PROSITE	PS51390. WAP. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	86241.
SOURCE	Search...

Entry information

Entry name

WFDC5_HUMAN

Accession

Primary (citable) accession number: Q8TCV5
Secondary accession number(s): Q5H981, Q6UWE4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2002
Last sequence update:	June 1, 2002
Last modified:	June 16, 2009
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)