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Q8TCU5 (NMD3A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate receptor ionotropic, NMDA 3A

Short name=GluN3A
Alternative name(s):
N-methyl-D-aspartate receptor subtype 3A
Short name=NMDAR3A
Short name=NR3A
NMDAR-L
Gene names
Name:GRIN3A
Synonyms:KIAA1973
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1115 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

NMDA receptor subtype of glutamate-gated ion channels with reduced single-channel conductance, low calcium permeability and low voltage-dependent sensitivity to magnesium. Mediated by glycine. May play a role in the development of dendritic spines. May play a role in PPP2CB-NMDAR mediated signaling mechanism By similarity.

Subunit structure

Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B). Does not form functional homomeric channels. Found in a complex with GRIN1, GRIN2A or GRIN2B and PPP2CB. Probably interacts with PPP2CB. No complex with PPP2CB is detected when NMDARs are stimulated by NMDA By similarity. Ref.7

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity. Cell junctionsynapsepostsynaptic cell membrane By similarity. Cell junctionsynapsepostsynaptic cell membranepostsynaptic density By similarity. Note: Enriched in postsynaptic plasma membrane and postsynaptic densities. Requires the presence of GRIN1 to be targeted at the plasma membrane By similarity.

Developmental stage

Expressed in fetal brain.

Post-translational modification

N-glycosylated By similarity.

Sequence similarities

Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR3A/GRIN3A subfamily. [View classification]

Sequence caution

The sequence BAB85559.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processIon transport
Transport
   Cellular componentCell junction
Cell membrane
Membrane
Postsynaptic cell membrane
Synapse
   Coding sequence diversityPolymorphism
   DomainCoiled coil
Signal
Transmembrane
Transmembrane helix
   LigandCalcium
Magnesium
   Molecular functionIon channel
Ligand-gated ion channel
Receptor
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcalcium ion transport

Inferred from sequence or structural similarity. Source: UniProtKB

dendrite development

Inferred from electronic annotation. Source: Ensembl

ion transmembrane transport

Inferred from Biological aspect of Ancestor. Source: RefGenome

ionotropic glutamate receptor signaling pathway

Inferred from Biological aspect of Ancestor. Source: RefGenome

prepulse inhibition

Inferred from electronic annotation. Source: Ensembl

response to ethanol

Inferred from direct assay PubMed 17502428PubMed 18445116. Source: UniProtKB

rhythmic process

Inferred from electronic annotation. Source: Ensembl

synaptic transmission, glutamatergic

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Cellular_componentN-methyl-D-aspartate selective glutamate receptor complex

Inferred from direct assay PubMed 17997397. Source: UniProtKB

cell junction

Inferred from electronic annotation. Source: UniProtKB-KW

dendrite

Inferred from Biological aspect of Ancestor. Source: RefGenome

integral component of membrane

Non-traceable author statement Ref.2. Source: UniProtKB

membrane

Inferred from direct assay PubMed 17997397. Source: UniProtKB

neuron projection

Inferred from direct assay PubMed 17658481. Source: UniProtKB

neuronal cell body

Inferred from direct assay PubMed 17658481. Source: UniProtKB

postsynaptic density

Inferred from electronic annotation. Source: UniProtKB-SubCell

postsynaptic membrane

Inferred from Biological aspect of Ancestor. Source: RefGenome

synapse

Inferred from direct assay PubMed 17658481. Source: UniProtKB

   Molecular_functionN-methyl-D-aspartate selective glutamate receptor activity

Inferred from direct assay PubMed 17502428. Source: UniProtKB

calcium channel activity

Inferred from electronic annotation. Source: Ensembl

extracellular-glutamate-gated ion channel activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

glycine binding

Inferred from direct assay PubMed 17320117. Source: UniProtKB

identical protein binding

Inferred from physical interaction PubMed 17997397. Source: UniProtKB

neurotransmitter binding

Inferred from electronic annotation. Source: Ensembl

protein phosphatase 2A binding

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 11151092Glutamate receptor ionotropic, NMDA 3A
PRO_0000011568

Regions

Topological domain24 – 674651Extracellular Potential
Transmembrane675 – 69521Helical; Potential
Topological domain696 – 74853Cytoplasmic Potential
Transmembrane749 – 76921Helical; Potential
Topological domain770 – 930161Extracellular Potential
Transmembrane931 – 95121Helical; Potential
Topological domain952 – 1115164Cytoplasmic Potential
Region951 – 98737PPP2CB binding site By similarity
Coiled coil1058 – 110952 Potential

Amino acid modifications

Glycosylation1451N-linked (GlcNAc...) Potential
Glycosylation2641N-linked (GlcNAc...) Potential
Glycosylation2751N-linked (GlcNAc...) Potential
Glycosylation2851N-linked (GlcNAc...) Potential
Glycosylation2961N-linked (GlcNAc...) Potential
Glycosylation4261N-linked (GlcNAc...) Potential
Glycosylation4391N-linked (GlcNAc...) Potential
Glycosylation5491N-linked (GlcNAc...) Potential
Glycosylation5651N-linked (GlcNAc...) Potential
Glycosylation8861N-linked (GlcNAc...) Potential

Natural variations

Natural variant3621V → M. Ref.2
Corresponds to variant rs10989591 [ dbSNP | Ensembl ].
VAR_019672
Natural variant4801R → H.
Corresponds to variant rs34755188 [ dbSNP | Ensembl ].
VAR_047150
Natural variant4871G → R. Ref.1 Ref.2
Corresponds to variant rs10989589 [ dbSNP | Ensembl ].
VAR_019673
Natural variant8351D → N. Ref.2
Corresponds to variant rs10989563 [ dbSNP | Ensembl ].
VAR_019674
Natural variant10411R → Q. Ref.3 Ref.5
Corresponds to variant rs3739722 [ dbSNP | Ensembl ].
VAR_019675

Experimental info

Sequence conflict5591S → N in AAL40734. Ref.1
Sequence conflict9771Q → R in AAL40734. Ref.1
Sequence conflict10731N → I in AAL40734. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q8TCU5 [UniParc].

Last modified November 4, 2008. Version 2.
Checksum: 0FEEC995F6AAF940

FASTA1,115125,465
        10         20         30         40         50         60 
MRRLSLWWLL SRVCLLLPPP CALVLAGVPS SSSHPQPCQI LKRIGHAVRV GAVHLQPWTT 

        70         80         90        100        110        120 
APRAASRAPD DSRAGAQRDE PEPGTRRSPA PSPGARWLGS TLHGRGPPGS RKPGEGARAE 

       130        140        150        160        170        180 
ALWPRDALLF AVDNLNRVEG LLPYNLSLEV VMAIEAGLGD LPLLPFSSPS SPWSSDPFSF 

       190        200        210        220        230        240 
LQSVCHTVVV QGVSALLAFP QSQGEMMELD LVSLVLHIPV ISIVRHEFPR ESQNPLHLQL 

       250        260        270        280        290        300 
SLENSLSSDA DVTVSILTMN NWYNFSLLLC QEDWNITDFL LLTQNNSKFH LGSIINITAN 

       310        320        330        340        350        360 
LPSTQDLLSF LQIQLESIKN STPTVVMFGC DMESIRRIFE ITTQFGVMPP ELRWVLGDSQ 

       370        380        390        400        410        420 
NVEELRTEGL PLGLIAHGKT TQSVFEHYVQ DAMELVARAV ATATMIQPEL ALIPSTMNCM 

       430        440        450        460        470        480 
EVETTNLTSG QYLSRFLANT TFRGLSGSIR VKGSTIVSSE NNFFIWNLQH DPMGKPMWTR 

       490        500        510        520        530        540 
LGSWQGGKIV MDYGIWPEQA QRHKTHFQHP SKLHLRVVTL IEHPFVFTRE VDDEGLCPAG 

       550        560        570        580        590        600 
QLCLDPMTND SSTLDSLFSS LHSSNDTVPI KFKKCCYGYC IDLLEKIAED MNFDFDLYIV 

       610        620        630        640        650        660 
GDGKYGAWKN GHWTGLVGDL LRGTAHMAVT SFSINTARSQ VIDFTSPFFS TSLGILVRTR 

       670        680        690        700        710        720 
DTAAPIGAFM WPLHWTMWLG IFVALHITAV FLTLYEWKSP FGLTPKGRNR SKVFSFSSAL 

       730        740        750        760        770        780 
NICYALLFGR TVAIKPPKCW TGRFLMNLWA IFCMFCLSTY TANLAAVMVG EKIYEELSGI 

       790        800        810        820        830        840 
HDPKLHHPSQ GFRFGTVRES SAEDYVRQSF PEMHEYMRRY NVPATPDGVE YLKNDPEKLD 

       850        860        870        880        890        900 
AFIMDKALLD YEVSIDADCK LLTVGKPFAI EGYGIGLPPN SPLTANISEL ISQYKSHGFM 

       910        920        930        940        950        960 
DMLHDKWYRV VPCGKRSFAV TETLQMGIKH FSGLFVLLCI GFGLSILTTI GEHIVYRLLL 

       970        980        990       1000       1010       1020 
PRIKNKSKLQ YWLHTSQRLH RAINTSFIEE KQQHFKTKRV EKRSNVGPRQ LTVWNTSNLS 

      1030       1040       1050       1060       1070       1080 
HDNRRKYIFS DEEGQNQLGI RIHQDIPLPP RRRELPALRT TNGKADSLNV SRNSVMQELS 

      1090       1100       1110 
ELEKQIQVIR QELQLAVSRK TELEEYQRTS RTCES 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence, genomic organization, and chromosomal localization of genes encoding the human NMDA receptor subunits NR3A and NR3B."
Andersson O., Stenqvist A., Attersand A., von Euler G.
Genomics 78:178-184(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ARG-487.
Tissue: Brain.
[2]"Cloning and expression of the human NMDA receptor subunit NR3A."
Eriksson M., Nilsson A., Froelich-Fabre S., Aakesson E., Dunker J., Seiger A., Folkesson R., Benedikz E., Sundstroem E.
Neurosci. Lett. 321:177-181(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS MET-362; ARG-487 AND ASN-835, TISSUE SPECIFICITY.
Tissue: Brain.
[3]"Prediction of the coding sequences of unidentified human genes. XXII. The complete sequences of 50 new cDNA clones which code for large proteins."
Nagase T., Kikuno R., Ohara O.
DNA Res. 8:319-327(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLN-1041.
Tissue: Brain.
[4]"DNA sequence and analysis of human chromosome 9."
Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. expand/collapse author list , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLN-1041.
[6]"Expression of the NR3A subunit of the NMDA receptor in human fetal brain."
Mueller H.T., Meador-Woodruff J.H.
Ann. N. Y. Acad. Sci. 1003:448-451(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[7]"An NMDA receptor signaling complex with protein phosphatase 2A."
Chan S.F., Sucher N.J.
J. Neurosci. 21:7985-7992(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PPP2CB.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF416558 mRNA. Translation: AAL40734.1.
AJ416950 mRNA. Translation: CAC95229.2.
AB075853 mRNA. Translation: BAB85559.1. Different initiation.
AL591377, AL356516 Genomic DNA. Translation: CAH69962.1.
AL356516, AL591377 Genomic DNA. Translation: CAI16651.1.
BC167432 mRNA. Translation: AAI67432.1.
RefSeqNP_597702.2. NM_133445.2.
UniGeneHs.654783.

3D structure databases

ProteinModelPortalQ8TCU5.
SMRQ8TCU5. Positions 143-497, 512-952.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid125508. 3 interactions.
MINTMINT-6630950.
STRING9606.ENSP00000355155.

Chemistry

BindingDBQ8TCU5.
ChEMBLCHEMBL4787.
DrugBankDB00659. Acamprosate.
DB01161. Chloroprocaine.
DB00514. Dextromethorphan.
DB00898. Ethanol.
DB00392. Ethopropazine.
DB00949. Felbamate.
DB01221. Ketamine.
DB00142. L-Glutamic Acid.
DB01043. Memantine.
DB00454. Meperidine.
DB00333. Methadone.
DB01173. Orphenadrine.
DB00721. Procaine.
DB00740. Riluzole.

PTM databases

PhosphoSiteQ8TCU5.

Polymorphism databases

DMDM212276445.

Proteomic databases

PaxDbQ8TCU5.
PRIDEQ8TCU5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000361820; ENSP00000355155; ENSG00000198785.
GeneID116443.
KEGGhsa:116443.
UCSCuc004bbp.2. human.

Organism-specific databases

CTD116443.
GeneCardsGC09M104331.
H-InvDBHIX0008248.
HIX0125606.
HGNCHGNC:16767. GRIN3A.
MIM606650. gene.
neXtProtNX_Q8TCU5.
PharmGKBPA28983.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG320645.
HOGENOMHOG000231528.
HOVERGENHBG052634.
InParanoidQ8TCU5.
KOK05213.
OMAMNCMEVE.
OrthoDBEOG7QK0B4.
PhylomeDBQ8TCU5.
TreeFamTF314731.

Gene expression databases

BgeeQ8TCU5.
CleanExHS_GRIN3A.
GenevestigatorQ8TCU5.

Family and domain databases

InterProIPR019594. Glu_rcpt_Glu/Gly-bd.
IPR001320. Iontro_glu_rcpt.
IPR001508. NMDA_rcpt.
IPR028082. Peripla_BP_I.
IPR001638. SBP_bac_3.
[Graphical view]
PfamPF00060. Lig_chan. 1 hit.
PF00497. SBP_bac_3. 1 hit.
[Graphical view]
PRINTSPR00177. NMDARECEPTOR.
SMARTSM00918. Lig_chan-Glu_bd. 1 hit.
SM00079. PBPe. 1 hit.
[Graphical view]
SUPFAMSSF53822. SSF53822. 1 hit.
ProtoNetSearch...

Other

GeneWikiGRIN3A.
GenomeRNAi116443.
NextBio79919.
PROQ8TCU5.
SOURCESearch...

Entry information

Entry nameNMD3A_HUMAN
AccessionPrimary (citable) accession number: Q8TCU5
Secondary accession number(s): B3DLF9 expand/collapse secondary AC list , Q5VTR3, Q8TF29, Q8WXI6
Entry history
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: November 4, 2008
Last modified: April 16, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 9

Human chromosome 9: entries, gene names and cross-references to MIM