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Reviewed, UniProtKB/Swiss-Prot Q8TCT1 (PHOP1_HUMAN)

Last modified June 16, 2009. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Phosphoethanolamine/phosphocholine phosphatase
    EC=3.1.3.75
Gene names
Name: PHOSPHO1
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length267 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Phosphatase that has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho). May be involved in the generation of inorganic phosphate for bone mineralization.

Catalytic activity

O-phosphoethanolamine + H2O = ethanolamine + phosphate. Ref.3

Phosphocholine + H2O = choline + phosphate. Ref.3

Cofactor

Magnesium. Ref.3

Tissue specificity

Expressed at sites of mineralization in bone and cartilage. Highly expressed in osteoblast cell line SaOS-2 which produces a mineralized matrix, but not in MG-63 cell line, which do not mineralize. Ref.4

Sequence similarities

Belongs to the HAD-like hydrolase superfamily. PHOSPHO family.

biophysicochemical properties

Kinetic parameters:

KM=3 µM for PEA

KM=11.4 µM for PCho

pH dependence:

Optimum pH is 6.7.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 267267Phosphoethanolamine/phosphocholine phosphatase
PRO_0000068829

Sites

Active site321Nucleophile Probable
Active site341Proton donor By similarity
Metal binding321Magnesium Probable
Metal binding341Magnesium By similarity
Metal binding2031Magnesium Probable
Binding site431Substrate Probable
Binding site1231Substrate Probable

Experimental info

Mutagenesis321D → N: Abolishes phosphatase activity. Ref.6
Mutagenesis431D → N: Strongly reduces reactivity toward PEA and PCho substrates. Abolishes phosphatase activity; when associated with N-123. Ref.6
Mutagenesis1231D → N: Strongly reduces reactivity toward PEA and PCho substrates. Abolishes phosphatase activity; when associated with N-43. Ref.6
Mutagenesis2031D → S: Abolishes phosphatase activity. Ref.6

Sequences

Sequence LengthMass (Da)Tools
Q8TCT1-1 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 539F65C749D39E81

FASTA26729,713
        10         20         30         40         50         60 
MSGCFPVSGL RCLSRDGRMA AQGAPRFLLT FDFDETIVDE NSDDSIVRAA PGQRLPESLR 

        70         80         90        100        110        120 
ATYREGFYNE YMQRVFKYLG EQGVRPRDLS AIYEAIPLSP GMSDLLQFVA KQGACFEVIL 

       130        140        150        160        170        180 
ISDANTFGVE SSLRAAGHHS LFRRILSNPS GPDARGLLAL RPFHTHSCAR CPANMCKHKV 

       190        200        210        220        230        240 
LSDYLRERAH DGVHFERLFY VGDGANDFCP MGLLAGGDVA FPRRGYPMHR LIQEAQKAEP 

       250        260 
SSFRASVVPW ETAADVRLHL QQVLKSC 

« Hide

References

« Hide 'large scale' references
[1]"Chromosomal localization of the chicken and mammalian orthologues of the orphan phosphatase PHOSPHO1 gene."
Houston B., Paton I.R., Burt D.W., Farquharson C.
Anim. Genet. 33:451-454(2002) [PubMed: 12464021] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Colon.
[3]"Human PHOSPHO1 exhibits high specific phosphoethanolamine and phosphocholine phosphatase activities."
Roberts S.J., Stewart A.J., Sadler P.J., Farquharson C.
Biochem. J. 382:59-65(2004) [PubMed: 15175005] [Abstract]
Cited for: ENZYME ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES.
[4]"PHOSPHO1 -- a novel phosphatase specifically expressed at sites of mineralisation in bone and cartilage."
Houston B., Stewart A.J., Farquharson C.
Bone 34:629-637(2004) [PubMed: 15050893] [Abstract]
Cited for: TISSUE SPECIFICITY.
[5]"Comparative modelling of human PHOSPHO1 reveals a new group of phosphatases within the haloacid dehalogenase superfamily."
Stewart A.J., Schmid R., Blindauer C.A., Paisey S.J., Farquharson C.
Protein Eng. 16:889-895(2003) [PubMed: 14983068] [Abstract]
Cited for: SIMILARITY TO HAD-LIKE HYDROLASE SUPERFAMILY.
[6]"Probing the substrate specificities of human PHOSPHO1 and PHOSPHO2."
Roberts S.J., Stewart A.J., Schmid R., Blindauer C.A., Bond S.R., Sadler P.J., Farquharson C.
Biochim. Biophys. Acta 1752:73-82(2005) [PubMed: 16054448] [Abstract]
Cited for: MUTAGENESIS OF ASP-32; ASP-43; ASP-123 AND ASP-203.
+Additional computationally mapped references.

Cross-references

Sequence databases

AJ457189 mRNA. Translation: CAD29803.1.
BC117187 mRNA. Translation: AAI17188.1.
IPIIPI00152434.
RefSeqNP_001137276.1.
NP_848595.1.
UniGeneHs.405607

3D structure databases

ModBaseSearch...

Proteomic databases

PRIDEQ8TCT1.

Genome annotation databases

EnsemblENSG00000173868. Homo sapiens. [Contig view]
GeneID162466.
KEGGhsa:162466.

Organism-specific databases

GeneCardsGC17M044655.
H-InvDBHIX0039112.
HGNCHGNC:16815. PHOSPHO1.
PharmGKBPA33276.
GenAtlasSearch...

Phylogenomic databases

HOGENOMQ8TCT1.
HOVERGENQ8TCT1.
OMAQ8TCT1. VAFPRRG.

Enzyme and pathway databases

BRENDA3.1.3.75. 247.

Gene expression databases

ArrayExpressQ8TCT1.
BgeeQ8TCT1.
CleanExHS_PHOSPHO1.
GermOnlineENSG00000173868. Homo sapiens.

Family and domain databases

InterProIPR006383. HAD-SF_hydro_IB_PSP-like.
IPR006384. PyrdxlP_Pase-rel.
IPR016965. PyrdxlP_Pase_PHOSPHO2.
[Graphical view]
PfamPF06888. Put_Phosphatase. 1 hit.
[Graphical view]
PIRSFPIRSF031051. PyrdxlP_Pase_PHOSPHO2. 1 hit.
TIGRFAMsTIGR01489. DKMTPPase-SF. 1 hit.
TIGR01488. HAD-SF-IB. 1 hit.
ProtoNetSearch...

Other Resources

DrugBankDB00122. Choline.
NextBio88177.

Entry information

Entry namePHOP1_HUMAN
AccessionPrimary (citable) accession number: Q8TCT1
Secondary accession number(s): Q17RU6
Entry history
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: June 1, 2002
Last modified: June 16, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents