Reviewed, UniProtKB/Swiss-Prot Q8TCT1 (PHOP1_HUMAN)

Names and origin

Protein names

Recommended name:
    Phosphoethanolamine/phosphocholine phosphatase
    EC=3.1.3.75

Gene names

Name:

PHOSPHO1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Phosphatase that has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho). May be involved in the generation of inorganic phosphate for bone mineralization.
Catalytic activity	O-phosphoethanolamine + H2O = ethanolamine + phosphate. Ref.3 Phosphocholine + H2O = choline + phosphate. Ref.3
Cofactor	Magnesium. Ref.3
Tissue specificity	Expressed at sites of mineralization in bone and cartilage. Highly expressed in osteoblast cell line SaOS-2 which produces a mineralized matrix, but not in MG-63 cell line, which do not mineralize. Ref.4
Sequence similarities	Belongs to the HAD-like hydrolase superfamily. PHOSPHO family.
biophysicochemical properties	Kinetic parameters: KM=3 µM for PEA KM=11.4 µM for PCho pH dependence: Optimum pH is 6.7.

Ontologies

Keywords
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoethanolamine/phosphocholine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 267	267	Phosphoethanolamine/phosphocholine phosphatase		PRO_0000068829
Sites
	Active site	32	1	Nucleophile Probable
	Active site	34	1	Proton donor By similarity
	Metal binding	32	1	Magnesium Probable
	Metal binding	34	1	Magnesium By similarity
	Metal binding	203	1	Magnesium Probable
	Binding site	43	1	Substrate Probable
	Binding site	123	1	Substrate Probable
Experimental info
	Mutagenesis	32	1	D → N: Abolishes phosphatase activity. Ref.6
	Mutagenesis	43	1	D → N: Strongly reduces reactivity toward PEA and PCho substrates. Abolishes phosphatase activity; when associated with N-123. Ref.6
	Mutagenesis	123	1	D → N: Strongly reduces reactivity toward PEA and PCho substrates. Abolishes phosphatase activity; when associated with N-43. Ref.6
	Mutagenesis	203	1	D → S: Abolishes phosphatase activity. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q8TCT1-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 539F65C749D39E81	FASTA	267	29,713
	10 20 30 40 50 60 MSGCFPVSGL RCLSRDGRMA AQGAPRFLLT FDFDETIVDE NSDDSIVRAA PGQRLPESLR 70 80 90 100 110 120 ATYREGFYNE YMQRVFKYLG EQGVRPRDLS AIYEAIPLSP GMSDLLQFVA KQGACFEVIL 130 140 150 160 170 180 ISDANTFGVE SSLRAAGHHS LFRRILSNPS GPDARGLLAL RPFHTHSCAR CPANMCKHKV 190 200 210 220 230 240 LSDYLRERAH DGVHFERLFY VGDGANDFCP MGLLAGGDVA FPRRGYPMHR LIQEAQKAEP 250 260 SSFRASVVPW ETAADVRLHL QQVLKSC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Chromosomal localization of the chicken and mammalian orthologues of the orphan phosphatase PHOSPHO1 gene." Houston B., Paton I.R., Burt D.W., Farquharson C. Anim. Genet. 33:451-454(2002) [PubMed: 12464021] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon.
[3]	"Human PHOSPHO1 exhibits high specific phosphoethanolamine and phosphocholine phosphatase activities." Roberts S.J., Stewart A.J., Sadler P.J., Farquharson C. Biochem. J. 382:59-65(2004) [PubMed: 15175005] [Abstract] Cited for: ENZYME ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES.
[4]	"PHOSPHO1 -- a novel phosphatase specifically expressed at sites of mineralisation in bone and cartilage." Houston B., Stewart A.J., Farquharson C. Bone 34:629-637(2004) [PubMed: 15050893] [Abstract] Cited for: TISSUE SPECIFICITY.
[5]	"Comparative modelling of human PHOSPHO1 reveals a new group of phosphatases within the haloacid dehalogenase superfamily." Stewart A.J., Schmid R., Blindauer C.A., Paisey S.J., Farquharson C. Protein Eng. 16:889-895(2003) [PubMed: 14983068] [Abstract] Cited for: SIMILARITY TO HAD-LIKE HYDROLASE SUPERFAMILY.
[6]	"Probing the substrate specificities of human PHOSPHO1 and PHOSPHO2." Roberts S.J., Stewart A.J., Schmid R., Blindauer C.A., Bond S.R., Sadler P.J., Farquharson C. Biochim. Biophys. Acta 1752:73-82(2005) [PubMed: 16054448] [Abstract] Cited for: MUTAGENESIS OF ASP-32; ASP-43; ASP-123 AND ASP-203.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ457189 mRNA. Translation: CAD29803.1. BC117187 mRNA. Translation: AAI17188.1.
IPI	IPI00152434.
RefSeq	NP_001137276.1. NP_848595.1.
UniGene	Hs.405607
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q8TCT1.
Genome annotation databases
Ensembl	ENSG00000173868. Homo sapiens. [Contig view]
GeneID	162466.
KEGG	hsa:162466.
Organism-specific databases
GeneCards	GC17M044655.
H-InvDB	HIX0039112.
HGNC	HGNC:16815. PHOSPHO1.
PharmGKB	PA33276.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q8TCT1.
HOVERGEN	Q8TCT1.
OMA	Q8TCT1. VAFPRRG.
Enzyme and pathway databases
BRENDA	3.1.3.75. 247.
Gene expression databases
ArrayExpress	Q8TCT1.
Bgee	Q8TCT1.
CleanEx	HS_PHOSPHO1.
GermOnline	ENSG00000173868. Homo sapiens.
Family and domain databases
InterPro	IPR006383. HAD-SF_hydro_IB_PSP-like. IPR006384. PyrdxlP_Pase-rel. IPR016965. PyrdxlP_Pase_PHOSPHO2. [Graphical view]
Pfam	PF06888. Put_Phosphatase. 1 hit. [Graphical view]
PIRSF	PIRSF031051. PyrdxlP_Pase_PHOSPHO2. 1 hit.
TIGRFAMs	TIGR01489. DKMTPPase-SF. 1 hit. TIGR01488. HAD-SF-IB. 1 hit.
ProtoNet	Search...
Other Resources
DrugBank	DB00122. Choline.
NextBio	88177.

Entry information

Entry name

PHOP1_HUMAN

Accession

Primary (citable) accession number: Q8TCT1
Secondary accession number(s): Q17RU6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	June 1, 2002
Last modified:	June 16, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)