Reviewed, UniProtKB/Swiss-Prot Q8TCJ2 (STT3B_HUMAN)

Names and origin

Protein names

Recommended name:
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B
      Short name=Oligosaccharyl transferase subunit STT3B
      Short name=STT3-B
    EC=2.4.1.119
Alternative name(s):
    Source of immunodominant MHC-associated peptides homolog

Gene names

Name:	STT3B
Synonyms:	SIMP

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	826 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). SST3B seems to be involved in complex substrate specificity By similarity.
Catalytic activity	Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.
Subunit structure	Component of the oligosaccharyltransferase (OST) complex By similarity. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein.
Tissue specificity	Expressed in heart, brain, placenta, lung, liver, muscle, kidney and pancreas. Expressed in skin fibroblasts (at protein level). Ref.4
Sequence similarities	Belongs to the STT3 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Transferase
PTM	Acetylation Glycoprotein Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid N-linked glycosylation via asparagine Ref.4 Inferred from direct assay. Source: HGNC
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell oligosaccharyltransferase complex Ref.4 Inferred from direct assay. Source: HGNC
Molecular function	dolichyl-diphosphooligosaccharide-protein glycotransferase activity Ref.4 Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RPN1	P04843	1	EBI-2256290,EBI-355963

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 826	825	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B		PRO_0000246001
Regions
	Transmembrane	65 – 85	21	Potential
	Transmembrane	137 – 157	21	Potential
	Transmembrane	169 – 189	21	Potential
	Transmembrane	192 – 212	21	Potential
	Transmembrane	243 – 259	17	Potential
	Transmembrane	263 – 283	21	Potential
	Transmembrane	292 – 312	21	Potential
	Transmembrane	320 – 340	21	Potential
	Transmembrane	351 – 371	21	Potential
	Transmembrane	411 – 431	21	Potential
	Transmembrane	441 – 461	21	Potential
	Transmembrane	464 – 484	21	Potential
	Transmembrane	538 – 558	21	Potential
Amino acid modifications
	Modified residue	2	1	N-acetylalanine Ref.9
	Modified residue	18	1	Phosphoserine Ref.7 Ref.12
	Modified residue	29	1	Phosphoserine Ref.7 Ref.12
	Modified residue	498	1	Phosphoserine Ref.7 Ref.6
	Modified residue	499	1	Phosphoserine Ref.7 Ref.6
	Glycosylation	623	1	N-linked (GlcNAc...) Ref.10
	Glycosylation	627	1	N-linked (GlcNAc...) Ref.10
	Glycosylation	641	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	822	1	S → F in AAH15880. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q8TCJ2-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: B70C221C7CBEB798	FASTA	826	93,674
	10 20 30 40 50 60 MAEPSAPESK HKSSLNSSPW SGLMALGNSR HGHHGPGAQC AHKAAGGAAP PKPAPAGLSG 70 80 90 100 110 120 GLSQPAGWQS LLSFTILFLA WLAGFSSRLF AVIRFESIIH EFDPWFNYRS THHLASHGFY 130 140 150 160 170 180 EFLNWFDERA WYPLGRIVGG TVYPGLMITA GLIHWILNTL NITVHIRDVC VFLAPTFSGL 190 200 210 220 230 240 TSISTFLLTR ELWNQGAGLL AACFIAIVPG YISRSVAGSF DNEGIAIFAL QFTYYLWVKS 250 260 270 280 290 300 VKTGSVFWTM CCCLSYFYMV SAWGGYVFII NLIPLHVFVL LLMQRYSKRV YIAYSTFYIV 310 320 330 340 350 360 GLILSMQIPF VGFQPIRTSE HMAAAGVFAL LQAYAFLQYL RDRLTKQEFQ TLFFLGVSLA 370 380 390 400 410 420 AGAVFLSVIY LTYTGYIAPW SGRFYSLWDT GYAKIHIPII ASVSEHQPTT WVSFFFDLHI 430 440 450 460 470 480 LVCTFPAGLW FCIKNINDER VFVALYAISA VYFAGVMVRL MLTLTPVVCM LSAIAFSNVF 490 500 510 520 530 540 EHYLGDDMKR ENPPVEDSSD EDDKRNQGNL YDKAGKVRKH ATEQEKTEEG LGPNIKSIVT 550 560 570 580 590 600 MLMLMLLMMF AVHCTWVTSN AYSSPSVVLA SYNHDGTRNI LDDFREAYFW LRQNTDEHAR 610 620 630 640 650 660 VMSWWDYGYQ IAGMANRTTL VDNNTWNNSH IALVGKAMSS NETAAYKIMR TLDVDYVLVI 670 680 690 700 710 720 FGGVIGYSGD DINKFLWMVR IAEGEHPKDI RESDYFTPQG EFRVDKAGSP TLLNCLMYKM 730 740 750 760 770 780 SYYRFGEMQL DFRTPPGFDR TRNAEIGNKD IKFKHLEEAF TSEHWLVRIY KVKAPDNRET 790 800 810 820 LDHKPRVTNI FPKQKYLSKK TTKRKRGYIK NKLVFKKGKK ISKKTV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The model B6dom1 minor histocompatibility antigen is encoded by a mouse homolog of the yeast STT3 gene." McBride K., Baron C., Picard S., Martin S., Boismenu D., Bell A., Bergeron J., Perreault C. Immunogenetics 54:562-569(2002) [PubMed: 12439619] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 510-826. Tissue: Placenta.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 576-826. Tissue: Placenta.
[4]	"Oligosaccharyltransferase isoforms that contain different catalytic STT3 subunits have distinct enzymatic properties." Kelleher D.J., Karaoglu D., Mandon E.C., Gilmore R. Mol. Cell 12:101-111(2003) [PubMed: 12887896] [Abstract] Cited for: COMPOSITION OF THE OLIGOSACCHARYLTRANSFERASE COMPLEX, TISSUE SPECIFICITY.
[5]	"Proteomic analysis of mammalian oligosaccharyltransferase reveals multiple subcomplexes that contain Sec61, TRAP, and two potential new subunits." Shibatani T., David L.L., McCormack A.L., Frueh K., Skach W.R. Biochemistry 44:5982-5992(2005) [PubMed: 15835887] [Abstract] Cited for: COMPOSITION OF THE OLIGOSACCHARYLTRANSFERASE COMPLEX.
[6]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-498 AND SER-499, MASS SPECTROMETRY. Tissue: Epithelium.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18; SER-29; SER-498 AND SER-499, MASS SPECTROMETRY.
[8]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[9]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, MASS SPECTROMETRY.
[10]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed: 19159218] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-623 AND ASN-627, MASS SPECTROMETRY. Tissue: Liver.
[11]	"Large-scale proteomics analysis of the human kinome." Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H. Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed: 19369195] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-498 AND SER-499, MASS SPECTROMETRY.
[12]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18 AND SER-29, MASS SPECTROMETRY. Tissue: T-cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY074880 mRNA. Translation: AAL71884.1. AK027789 mRNA. Translation: BAB55370.1. Different initiation. AK075380 mRNA. Translation: BAC11581.1. Different initiation. BC015880 mRNA. Translation: AAH15880.1. Different initiation.
IPI	IPI00152377.
RefSeq	NP_849193.1.
UniGene	Hs.475812
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8TCJ2. 1 interaction.
STRING	Q8TCJ2.
Protein family/group databases
CAZy	GT66. Glycosyltransferase Family 66.
PTM databases
PhosphoSite	Q8TCJ2.
Proteomic databases
PeptideAtlas	Q8TCJ2.
PRIDE	Q8TCJ2.
Genome annotation databases
Ensembl	ENST00000295770; ENSP00000295770; ENSG00000163527; Homo sapiens. [Genome view]
GeneID	201595.
KEGG	hsa:201595.
UCSC	uc003ces.1. human.
Organism-specific databases
CTD	201595.
GeneCards	GC03P031550.
H-InvDB	HIX0003144.
HGNC	HGNC:30611. STT3B.
MIM	608605. gene.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG15633.
HOGENOM	HBG314442.
HOVERGEN	Q8TCJ2.
InParanoid	Q8TCJ2.
OMA	XYIAPWS.
OrthoDB	EOG9FBMD4.
PhylomeDB	Q8TCJ2.
Enzyme and pathway databases
BRENDA	2.4.1.119. 247.
Gene expression databases
ArrayExpress	Q8TCJ2.
Bgee	Q8TCJ2.
CleanEx	HS_STT3B.
Genevestigator	Q8TCJ2.
GermOnline	ENSG00000163527. Homo sapiens.
Family and domain databases
InterPro	IPR003674. Oligo_trans_STT3. [Graphical view]
Pfam	PF02516. STT3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	90168.
SOURCE	Search...

Entry information

Entry name

STT3B_HUMAN

Accession

Primary (citable) accession number: Q8TCJ2
Secondary accession number(s): Q96JZ4, Q96KY7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
Last sequence update:	June 1, 2002
Last modified:	February 9, 2010
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.