Reviewed,
UniProtKB/Swiss-Prot Q8TCJ2 (STT3B_HUMAN)
Last modified
February 9, 2010.
Version 54.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B Short name=Oligosaccharyl transferase subunit STT3B Short name=STT3-B EC=2.4.1.119 Alternative name(s): Source of immunodominant MHC-associated peptides homolog | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 826 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Component of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). SST3B seems to be involved in complex substrate specificity By similarity. |
| Catalytic activity | Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine. |
| Subunit structure | Component of the oligosaccharyltransferase (OST) complex By similarity. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes. |
| Subcellular location | Endoplasmic reticulum membrane; Multi-pass membrane protein. |
| Tissue specificity | Expressed in heart, brain, placenta, lung, liver, muscle, kidney and pancreas. Expressed in skin fibroblasts (at protein level). Ref.4 |
| Sequence similarities | Belongs to the STT3 family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Endoplasmic reticulum Membrane |
| Domain | Transmembrane |
| Molecular function | Transferase |
| PTM | Acetylation Glycoprotein Phosphoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | protein amino acid N-linked glycosylation via asparagine Ref.4 Inferred from direct assay. Source: HGNC |
| Cellular component | integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell oligosaccharyltransferase complex Ref.4Inferred from direct assay. Source: HGNC |
| Molecular function | dolichyl-diphosphooligosaccharide-protein glycotransferase activity Ref.4 Inferred from electronic annotation. Source: EC protein bindingInferred from physical interaction. Source: IntAct |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed | ||||||
| Chain | 2 – 826 | 825 | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B | PRO_0000246001 | |||||
Regions | |||||||||
| Transmembrane | 65 – 85 | 21 | Potential | ||||||
| Transmembrane | 137 – 157 | 21 | Potential | ||||||
| Transmembrane | 169 – 189 | 21 | Potential | ||||||
| Transmembrane | 192 – 212 | 21 | Potential | ||||||
| Transmembrane | 243 – 259 | 17 | Potential | ||||||
| Transmembrane | 263 – 283 | 21 | Potential | ||||||
| Transmembrane | 292 – 312 | 21 | Potential | ||||||
| Transmembrane | 320 – 340 | 21 | Potential | ||||||
| Transmembrane | 351 – 371 | 21 | Potential | ||||||
| Transmembrane | 411 – 431 | 21 | Potential | ||||||
| Transmembrane | 441 – 461 | 21 | Potential | ||||||
| Transmembrane | 464 – 484 | 21 | Potential | ||||||
| Transmembrane | 538 – 558 | 21 | Potential | ||||||
Amino acid modifications | |||||||||
| Modified residue | 2 | 1 | N-acetylalanine Ref.9 | ||||||
| Modified residue | 18 | 1 | Phosphoserine Ref.7 Ref.12 | ||||||
| Modified residue | 29 | 1 | Phosphoserine Ref.7 Ref.12 | ||||||
| Modified residue | 498 | 1 | Phosphoserine Ref.7 Ref.6 | ||||||
| Modified residue | 499 | 1 | Phosphoserine Ref.7 Ref.6 | ||||||
| Glycosylation | 623 | 1 | N-linked (GlcNAc...) Ref.10 | ||||||
| Glycosylation | 627 | 1 | N-linked (GlcNAc...) Ref.10 | ||||||
| Glycosylation | 641 | 1 | N-linked (GlcNAc...) Potential | ||||||
Experimental info | |||||||||
| Sequence conflict | 822 | 1 | S → F in AAH15880. Ref.3 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The model B6dom1 minor histocompatibility antigen is encoded by a mouse homolog of the yeast STT3 gene." McBride K., Baron C., Picard S., Martin S., Boismenu D., Bell A., Bergeron J., Perreault C. Immunogenetics 54:562-569(2002) [PubMed: 12439619] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 510-826. Tissue: Placenta. |
| [3] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 576-826. Tissue: Placenta. |
| [4] | "Oligosaccharyltransferase isoforms that contain different catalytic STT3 subunits have distinct enzymatic properties." Kelleher D.J., Karaoglu D., Mandon E.C., Gilmore R. Mol. Cell 12:101-111(2003) [PubMed: 12887896] [Abstract] Cited for: COMPOSITION OF THE OLIGOSACCHARYLTRANSFERASE COMPLEX, TISSUE SPECIFICITY. |
| [5] | "Proteomic analysis of mammalian oligosaccharyltransferase reveals multiple subcomplexes that contain Sec61, TRAP, and two potential new subunits." Shibatani T., David L.L., McCormack A.L., Frueh K., Skach W.R. Biochemistry 44:5982-5992(2005) [PubMed: 15835887] [Abstract] Cited for: COMPOSITION OF THE OLIGOSACCHARYLTRANSFERASE COMPLEX. |
| [6] | "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-498 AND SER-499, MASS SPECTROMETRY. Tissue: Epithelium. |
| [7] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18; SER-29; SER-498 AND SER-499, MASS SPECTROMETRY. |
| [8] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| [9] | "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, MASS SPECTROMETRY. |
| [10] | "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed: 19159218] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-623 AND ASN-627, MASS SPECTROMETRY. Tissue: Liver. |
| [11] | "Large-scale proteomics analysis of the human kinome." Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H. Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed: 19369195] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-498 AND SER-499, MASS SPECTROMETRY. |
| [12] | "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18 AND SER-29, MASS SPECTROMETRY. Tissue: T-cell. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AY074880 mRNA. Translation: AAL71884.1. AK027789 mRNA. Translation: BAB55370.1. Different initiation. AK075380 mRNA. Translation: BAC11581.1. Different initiation. BC015880 mRNA. Translation: AAH15880.1. Different initiation. |
| IPI | IPI00152377. |
| RefSeq | NP_849193.1. |
| UniGene | Hs.475812 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q8TCJ2. 1 interaction. |
| STRING | Q8TCJ2. |
Protein family/group databases | |
| CAZy | GT66. Glycosyltransferase Family 66. |
PTM databases | |
| PhosphoSite | Q8TCJ2. |
Proteomic databases | |
| PeptideAtlas | Q8TCJ2. |
| PRIDE | Q8TCJ2. |
Genome annotation databases | |
| Ensembl | ENST00000295770; ENSP00000295770; ENSG00000163527; Homo sapiens. [Genome view] |
| GeneID | 201595. |
| KEGG | hsa:201595. |
| UCSC | uc003ces.1. human. |
Organism-specific databases | |
| CTD | 201595. |
| GeneCards | GC03P031550. |
| H-InvDB | HIX0003144. |
| HGNC | HGNC:30611. STT3B. |
| MIM | 608605. gene. |
| GenAtlas | Search... |
Phylogenomic databases | |
| eggNOG | prNOG15633. |
| HOGENOM | HBG314442. |
| HOVERGEN | Q8TCJ2. |
| InParanoid | Q8TCJ2. |
| OMA | XYIAPWS. |
| OrthoDB | EOG9FBMD4. |
| PhylomeDB | Q8TCJ2. |
Enzyme and pathway databases | |
| BRENDA | 2.4.1.119. 247. |
Gene expression databases | |
| ArrayExpress | Q8TCJ2. |
| Bgee | Q8TCJ2. |
| CleanEx | HS_STT3B. |
| Genevestigator | Q8TCJ2. |
| GermOnline | ENSG00000163527. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR003674. Oligo_trans_STT3. [Graphical view] |
| Pfam | PF02516. STT3. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 90168. |
| SOURCE | Search... |
Entry information
| Entry name | STT3B_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q8TCJ2 Secondary accession number(s): Q96JZ4, Q96KY7 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 3 Human chromosome 3: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


