Reviewed, UniProtKB/Swiss-Prot Q8TCJ0 (FBX25_HUMAN)

Names and origin

Protein names

Recommended name:
    F-box only protein 25

Gene names

Name:	FBXO25
Synonyms:	FBX25

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.
Subunit structure	Directly interacts with SKP1A and CUL1.
Subcellular location	Nucleus.
Tissue specificity	Expressed in all brain tissue observed.
Involvement in disease	A chromosomal aberration involving FBXO25 is a cause of X-linked mental retardation (XLMR). Translocation t(X;8)(p11.22;p23.3) with SHROOM4.
Sequence similarities	Contains 1 F-box domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Chromosomal rearrangement
Gene Ontology (GO)
Biological process	protein ubiquitination Ref.4 Non-traceable author statement. Source: UniProtKB ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin ligase complex Ref.4 Non-traceable author statement. Source: UniProtKB
Molecular function	ubiquitin-protein ligase activity Ref.4 Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8TCJ0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Notes: No experimental confirmation available.

Isoform 2 (identifier: Q8TCJ0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     331-339: Missing.

Isoform 3 (identifier: Q8TCJ0-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-67: Missing.
     68-96: KKDHFRNDTNTQSFYREKWIYVHKESTKE → MEKYSIMKSMNMHRKKGKRTILEMTQILK
     331-339: Missing.

Notes: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 367	367	F-box only protein 25		PRO_0000119911
Regions
	Domain	226 – 274	49	F-box
Natural variations
	Alternative sequence	1 – 67	67	Missing in isoform 3.		VSP_013060
	Alternative sequence	68 – 96	29	KKDHF…ESTKE → MEKYSIMKSMNMHRKKGKRT ILEMTQILK in isoform 3.		VSP_013061
	Alternative sequence	331 – 339	9	Missing in isoform 2 and isoform 3.		VSP_007374
Experimental info
	Mutagenesis	244	1	S → L: Loss of SKP1 binding
	Sequence conflict	38	1	R → H in AAH50393. Ref.3
	Sequence conflict	87 – 96	10	IYVHKESTKE → LILTSVLLFQ Ref.4
	Sequence conflict	121	1	R → T in AAF04526. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 15, 2005. Version 3. Checksum: 1C1C6AD43A92754F	FASTA	367	43,313
	10 20 30 40 50 60 MPFLGQDWRS PGWSWIKTED GWKRCESCSQ KLERENNRCN ISHSIILNSE DGEIFNNEEH 70 80 90 100 110 120 EYASKKRKKD HFRNDTNTQS FYREKWIYVH KESTKERHGY CTLGEAFNRL DFSSAIQDIR 130 140 150 160 170 180 RFNYVVKLLQ LIAKSQLTSL SGVAQKNYFN ILDKIVQKVL DDHHNPRLIK DLLQDLSSTL 190 200 210 220 230 240 CILIRGVGKS VLVGNINIWI CRLETILAWQ QQLQDLQMTK QVNNGLTLSD LPLHMLNNIL 250 260 270 280 290 300 YRFSDGWDII TLGQVTPTLY MLSEDRQLWK KLCQYHFAEK QFCRHLILSE KGHIEWKLMY 310 320 330 340 350 360 FALQKHYPAK EQYGDTLHFC RHCSILFWKD YHLALLFKDS GHPCTAADPD SCFTPVSPQH FIDLFKF
	Isoform 2 [UniParc]. Checksum: 11CCEFCA20F3BF37		358	42,211
	10 20 30 40 50 60 MPFLGQDWRS PGWSWIKTED GWKRCESCSQ KLERENNRCN ISHSIILNSE DGEIFNNEEH 70 80 90 100 110 120 EYASKKRKKD HFRNDTNTQS FYREKWIYVH KESTKERHGY CTLGEAFNRL DFSSAIQDIR 130 140 150 160 170 180 RFNYVVKLLQ LIAKSQLTSL SGVAQKNYFN ILDKIVQKVL DDHHNPRLIK DLLQDLSSTL 190 200 210 220 230 240 CILIRGVGKS VLVGNINIWI CRLETILAWQ QQLQDLQMTK QVNNGLTLSD LPLHMLNNIL 250 260 270 280 290 300 YRFSDGWDII TLGQVTPTLY MLSEDRQLWK KLCQYHFAEK QFCRHLILSE KGHIEWKLMY 310 320 330 340 350 FALQKHYPAK EQYGDTLHFC RHCSILFWKD SGHPCTAADP DSCFTPVSPQ HFIDLFKF
	Isoform 3 [UniParc]. Checksum: 4222DEAEA61A7487		291	34,067
	10 20 30 40 50 60 MEKYSIMKSM NMHRKKGKRT ILEMTQILKR HGYCTLGEAF NRLDFSSAIQ DIRRFNYVVK 70 80 90 100 110 120 LLQLIAKSQL TSLSGVAQKN YFNILDKIVQ KVLDDHHNPR LIKDLLQDLS STLCILIRGV 130 140 150 160 170 180 GKSVLVGNIN IWICRLETIL AWQQQLQDLQ MTKQVNNGLT LSDLPLHMLN NILYRFSDGW 190 200 210 220 230 240 DIITLGQVTP TLYMLSEDRQ LWKKLCQYHF AEKQFCRHLI LSEKGHIEWK LMYFALQKHY 250 260 270 280 290 PAKEQYGDTL HFCRHCSILF WKDSGHPCTA ADPDSCFTPV SPQHFIDLFK F

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Guo J.H., She X.Y., Yu L. Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[2]	"Expression of Fbx25-containing protein in prostate cancer tissues." Tsujiawa K., Mitsui E., Ono Y., Sakamoto K., Konishi N., Yamamoto H. Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Prostatic carcinoma.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Tissue: Brain and Placenta.
[4]	"Identification of a family of human F-box proteins." Cenciarelli C., Chiaur D.S., Guardavaccaro D., Parks W., Vidal M., Pagano M. Curr. Biol. 9:1177-1179(1999) [PubMed: 10531035] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 87-367 (ISOFORM 2).
[5]	"Characterization of FBX25, encoding a novel brain-expressed F-box protein." Hagens O., Minina E., Schweiger S., Ropers H.-H., Kalscheuer V. Biochim. Biophys. Acta 1760:110-118(2006) [PubMed: 16278047] [Abstract] Cited for: INTERACTION WITH SKP1A AND CUL1, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, MUTAGENESIS OF SER-244.
[6]	"Disruptions of the novel KIAA1202 gene are associated with X-linked mental retardation." Hagens O., Dubos A., Abidi F., Barbi G., Van Zutven L., Hoeltzenbein M., Tommerup N., Moraine C., Fryns J.-P., Chelly J., van Bokhoven H., Gecz J., Dollfus H., Ropers H.-H., Schwartz C.E., de Cassia Stocco Dos Santos R., Kalscheuer V., Hanauer A. Hum. Genet. 118:578-590(2006) [PubMed: 16249884] [Abstract] Cited for: CHROMOSOMAL TRANSLOCATION WITH SHROOM4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF419858 mRNA. Translation: AAP97293.1. AB070270 mRNA. Translation: BAB85128.1. BC020249 mRNA. Translation: AAH20249.1. BC050393 mRNA. Translation: AAH50393.1. AF174605 mRNA. Translation: AAF04526.1.
RefSeq	NP_036305.2. NP_904356.1. NP_904357.1.
UniGene	Hs.438454
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q8TCJ0.
Genome annotation databases
Ensembl	ENSG00000147364. Homo sapiens. [Contig view]
GeneID	26260.
KEGG	hsa:26260.
Organism-specific databases
HGNC	HGNC:13596. FBXO25.
HPA	HPA002553.
MIM	609098. gene.
PharmGKB	PA28038.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	Q8TCJ0.
HOVERGEN	Q8TCJ0.
Gene expression databases
ArrayExpress	Q8TCJ0.
CleanEx	HS_FBXO25.
GermOnline	ENSG00000147364. Homo sapiens.
Family and domain databases
InterPro	IPR001810. F-box. [Graphical view]
Pfam	PF00646. F-box. 1 hit. [Graphical view]
PROSITE	PS50181. FBOX. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
LinkHub	Q8TCJ0.
NextBio	48509.
SOURCE	Search...

Entry information

Entry name

FBX25_HUMAN

Accession

Primary (citable) accession number: Q8TCJ0
Secondary accession number(s): Q6PJ83, Q7Z4V4, Q9UKB8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 9, 2003
Last sequence update:	March 15, 2005
Last modified:	November 25, 2008
This is version 53 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)