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Q8TCJ0 (FBX25_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
F-box only protein 25
Gene names
Name:FBXO25
Synonyms:FBX25
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length367 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. May play a role in accumulation of expanded polyglutamine (polyQ) protein huntingtin (HTT) By similarity.

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Part of a SCF (SKP1-cullin-F-box) protein ligase complex consisting of FBXO25, SKP1, CUL1 and RBX1. Interacts directly with SKP1 and CUL1. Interacts (via C-terminus) with beta-actin (via N-terminus). Ref.5 Ref.6

Subcellular location

Nucleus. Note: In the nucleus, associates with a subnuclear dot-like structure. Colocalized with SKP1. Ref.6 Ref.8

Tissue specificity

Expressed in all brain tissue observed. Ref.6

Domain

The F-box is necessary for the interaction with SKP1 By similarity.

Involvement in disease

A chromosomal aberration involving FBXO25 is a cause of X-linked mental retardation (XLMR). Translocation t(X;8)(p11.22;p23.3) with SHROOM4.

Sequence similarities

Contains 1 F-box domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ACTBP607093EBI-6264551,EBI-353944

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8TCJ0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available.
Isoform 2 (identifier: Q8TCJ0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     331-339: Missing.
Isoform 3 (identifier: Q8TCJ0-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-67: Missing.
     68-96: KKDHFRNDTNTQSFYREKWIYVHKESTKE → MEKYSIMKSMNMHRKKGKRTILEMTQILK
     331-339: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 367367F-box only protein 25
PRO_0000119911

Regions

Domain226 – 27449F-box
Region1 – 8383Interaction with beta-actin

Natural variations

Alternative sequence1 – 6767Missing in isoform 3.
VSP_013060
Alternative sequence68 – 9629KKDHF…ESTKE → MEKYSIMKSMNMHRKKGKRT ILEMTQILK in isoform 3.
VSP_013061
Alternative sequence331 – 3399Missing in isoform 2 and isoform 3.
VSP_007374
Natural variant361N → D.
Corresponds to variant rs17665340 [ dbSNP | Ensembl ].
VAR_049043
Natural variant381R → H. Ref.3
Corresponds to variant rs10090550 [ dbSNP | Ensembl ].
VAR_061167

Experimental info

Mutagenesis2441S → L: Loss of SKP1-binding. Ref.6
Sequence conflict87 – 9610IYVHKESTKE → LILTSVLLFQ in AAF04526. Ref.4
Sequence conflict1211R → T in AAF04526. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 15, 2005. Version 3.
Checksum: 1C1C6AD43A92754F

FASTA36743,313
        10         20         30         40         50         60 
MPFLGQDWRS PGWSWIKTED GWKRCESCSQ KLERENNRCN ISHSIILNSE DGEIFNNEEH 

        70         80         90        100        110        120 
EYASKKRKKD HFRNDTNTQS FYREKWIYVH KESTKERHGY CTLGEAFNRL DFSSAIQDIR 

       130        140        150        160        170        180 
RFNYVVKLLQ LIAKSQLTSL SGVAQKNYFN ILDKIVQKVL DDHHNPRLIK DLLQDLSSTL 

       190        200        210        220        230        240 
CILIRGVGKS VLVGNINIWI CRLETILAWQ QQLQDLQMTK QVNNGLTLSD LPLHMLNNIL 

       250        260        270        280        290        300 
YRFSDGWDII TLGQVTPTLY MLSEDRQLWK KLCQYHFAEK QFCRHLILSE KGHIEWKLMY 

       310        320        330        340        350        360 
FALQKHYPAK EQYGDTLHFC RHCSILFWKD YHLALLFKDS GHPCTAADPD SCFTPVSPQH 


FIDLFKF 

« Hide

Isoform 2 [UniParc].

Checksum: 11CCEFCA20F3BF37
Show »

FASTA35842,211
Isoform 3 [UniParc].

Checksum: 4222DEAEA61A7487
Show »

FASTA29134,067

References

« Hide 'large scale' references
[1]"Expression of Fbx25-containing protein in prostate cancer tissues."
Tsujiawa K., Mitsui E., Ono Y., Sakamoto K., Konishi N., Yamamoto H.
Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Tissue: Prostatic carcinoma.
[2]Guo J.H., She X.Y., Yu L.
Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3), VARIANT HIS-38.
Tissue: Brain and Placenta.
[4]"Identification of a family of human F-box proteins."
Cenciarelli C., Chiaur D.S., Guardavaccaro D., Parks W., Vidal M., Pagano M.
Curr. Biol. 9:1177-1179(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 87-367 (ISOFORM 2).
[5]"Identification of FBXO25-interacting proteins using an integrated proteomics approach."
Teixeira F.R., Yokoo S., Gartner C.A., Manfiolli A.O., Baqui M.M., Assmann E.M., Maragno A.L., Yu H., de Lanerolle P., Kobarg J., Gygi S.P., Gomes M.D.
Proteomics 10:2746-2757(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 110-120; 135-146; 147-154; 190-202 AND 339-366, INTERACTION WITH BETA-ACTIN AND SKP1, IDENTIFICATION IN A SCF PROTEIN LIGASE COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
[6]"Characterization of FBX25, encoding a novel brain-expressed F-box protein."
Hagens O., Minina E., Schweiger S., Ropers H.-H., Kalscheuer V.
Biochim. Biophys. Acta 1760:110-118(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SKP1 AND CUL1, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, MUTAGENESIS OF SER-244.
[7]"Disruptions of the novel KIAA1202 gene are associated with X-linked mental retardation."
Hagens O., Dubos A., Abidi F., Barbi G., Van Zutven L., Hoeltzenbein M., Tommerup N., Moraine C., Fryns J.-P., Chelly J., van Bokhoven H., Gecz J., Dollfus H., Ropers H.-H., Schwartz C.E., de Cassia Stocco Dos Santos R., Kalscheuer V., Hanauer A.
Hum. Genet. 118:578-590(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: CHROMOSOMAL TRANSLOCATION WITH SHROOM4.
[8]"FBXO25-associated nuclear domains: a novel subnuclear structure."
Manfiolli A.O., Maragno A.L., Baqui M.M., Yokoo S., Teixeira F.R., Oliveira E.B., Gomes M.D.
Mol. Biol. Cell 19:1848-1861(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB070270 mRNA. Translation: BAB85128.1.
AF419858 mRNA. Translation: AAP97293.1.
BC020249 mRNA. Translation: AAH20249.1.
BC050393 mRNA. Translation: AAH50393.1.
AF174605 mRNA. Translation: AAF04526.1.
CCDSCCDS5952.1. [Q8TCJ0-2]
CCDS5953.1. [Q8TCJ0-1]
CCDS5954.1. [Q8TCJ0-3]
RefSeqNP_036305.2. NM_012173.3. [Q8TCJ0-3]
NP_904356.1. NM_183420.1. [Q8TCJ0-2]
NP_904357.1. NM_183421.1. [Q8TCJ0-1]
UniGeneHs.438454.

3D structure databases

ProteinModelPortalQ8TCJ0.
SMRQ8TCJ0. Positions 228-305.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid117646. 190 interactions.
IntActQ8TCJ0. 7 interactions.
STRING9606.ENSP00000276326.

PTM databases

PhosphoSiteQ8TCJ0.

Proteomic databases

MaxQBQ8TCJ0.
PaxDbQ8TCJ0.
PRIDEQ8TCJ0.

Protocols and materials databases

DNASU26260.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000276326; ENSP00000276326; ENSG00000147364. [Q8TCJ0-1]
ENST00000350302; ENSP00000342077; ENSG00000147364. [Q8TCJ0-2]
ENST00000352684; ENSP00000341345; ENSG00000147364. [Q8TCJ0-3]
ENST00000382824; ENSP00000372274; ENSG00000147364. [Q8TCJ0-3]
GeneID26260.
KEGGhsa:26260.
UCSCuc003wox.3. human. [Q8TCJ0-1]
uc003woy.3. human. [Q8TCJ0-2]
uc003woz.3. human. [Q8TCJ0-3]

Organism-specific databases

CTD26260.
GeneCardsGC08P000346.
H-InvDBHIX0057066.
HGNCHGNC:13596. FBXO25.
MIM609098. gene.
neXtProtNX_Q8TCJ0.
PharmGKBPA28038.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG327896.
HOGENOMHOG000263478.
HOVERGENHBG051572.
InParanoidQ8TCJ0.
KOK10305.
OMADWKLMYF.
PhylomeDBQ8TCJ0.
TreeFamTF313070.

Enzyme and pathway databases

UniPathwayUPA00143.

Gene expression databases

ArrayExpressQ8TCJ0.
BgeeQ8TCJ0.
CleanExHS_FBXO25.
GenevestigatorQ8TCJ0.

Family and domain databases

InterProIPR001810. F-box_dom.
[Graphical view]
SUPFAMSSF81383. SSF81383. 1 hit.
ProtoNetSearch...

Other

GenomeRNAi26260.
NextBio48509.
PROQ8TCJ0.
SOURCESearch...

Entry information

Entry nameFBX25_HUMAN
AccessionPrimary (citable) accession number: Q8TCJ0
Secondary accession number(s): Q6PJ83, Q7Z4V4, Q9UKB8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 9, 2003
Last sequence update: March 15, 2005
Last modified: July 9, 2014
This is version 103 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 8

Human chromosome 8: entries, gene names and cross-references to MIM