Q8TCJ0 (FBX25_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
F-box only protein 25

Gene names

Name:	FBXO25
Synonyms:	FBX25

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. May play a role in accumulation of expanded polyglutamine (polyQ) protein huntingtin (HTT) By similarity.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Part of a SCF (SKP1-cullin-F-box) protein ligase complex consisting of FBXO25, SKP1, CUL1 and RBX1. Interacts directly with SKP1 and CUL1. Interacts (via C-terminus) with beta-actin (via N-terminus). Ref.5 Ref.6
Subcellular location	Nucleus. Note: In the nucleus, associates with a subnuclear dot-like structure. Colocalized with SKP1. Ref.6 Ref.8
Tissue specificity	Expressed in all brain tissue observed. Ref.6
Domain	The F-box is necessary for the interaction with SKP1 By similarity.
Involvement in disease	A chromosomal aberration involving FBXO25 is a cause of X-linked mental retardation (XLMR). Translocation t(X;8)(p11.22;p23.3) with SHROOM4.
Sequence similarities	Contains 1 F-box domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Chromosomal rearrangement Polymorphism
Ligand	Actin-binding
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	SCF ubiquitin ligase complex Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	ubiquitin-protein ligase activity Non-traceable author statement Ref.4. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ACTB	P60709	3	EBI-6264551,EBI-353944

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8TCJ0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform 2 (identifier: Q8TCJ0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     331-339: Missing.

Isoform 3 (identifier: Q8TCJ0-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-67: Missing.
     68-96: KKDHFRNDTNTQSFYREKWIYVHKESTKE → MEKYSIMKSMNMHRKKGKRTILEMTQILK
     331-339: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 367	367	F-box only protein 25		PRO_0000119911
Regions
	Domain	226 – 274	49	F-box
	Region	1 – 83	83	Interaction with beta-actin
Natural variations
	Alternative sequence	1 – 67	67	Missing in isoform 3.		VSP_013060
	Alternative sequence	68 – 96	29	KKDHF…ESTKE → MEKYSIMKSMNMHRKKGKRT ILEMTQILK in isoform 3.		VSP_013061
	Alternative sequence	331 – 339	9	Missing in isoform 2 and isoform 3.		VSP_007374
	Natural variant	36	1	N → D. Corresponds to variant rs17665340 [ dbSNP | Ensembl ].		VAR_049043
	Natural variant	38	1	R → H. Ref.3 Corresponds to variant rs10090550 [ dbSNP | Ensembl ].		VAR_061167
Experimental info
	Mutagenesis	244	1	S → L: Loss of SKP1-binding. Ref.6
	Sequence conflict	87 – 96	10	IYVHKESTKE → LILTSVLLFQ in AAF04526. Ref.4
	Sequence conflict	121	1	R → T in AAF04526. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 15, 2005. Version 3. Checksum: 1C1C6AD43A92754F	FASTA	367	43,313
	10 20 30 40 50 60 MPFLGQDWRS PGWSWIKTED GWKRCESCSQ KLERENNRCN ISHSIILNSE DGEIFNNEEH 70 80 90 100 110 120 EYASKKRKKD HFRNDTNTQS FYREKWIYVH KESTKERHGY CTLGEAFNRL DFSSAIQDIR 130 140 150 160 170 180 RFNYVVKLLQ LIAKSQLTSL SGVAQKNYFN ILDKIVQKVL DDHHNPRLIK DLLQDLSSTL 190 200 210 220 230 240 CILIRGVGKS VLVGNINIWI CRLETILAWQ QQLQDLQMTK QVNNGLTLSD LPLHMLNNIL 250 260 270 280 290 300 YRFSDGWDII TLGQVTPTLY MLSEDRQLWK KLCQYHFAEK QFCRHLILSE KGHIEWKLMY 310 320 330 340 350 360 FALQKHYPAK EQYGDTLHFC RHCSILFWKD YHLALLFKDS GHPCTAADPD SCFTPVSPQH FIDLFKF
	Isoform 2 [UniParc]. Checksum: 11CCEFCA20F3BF37	FASTA	358	42,211
	10 20 30 40 50 60 MPFLGQDWRS PGWSWIKTED GWKRCESCSQ KLERENNRCN ISHSIILNSE DGEIFNNEEH 70 80 90 100 110 120 EYASKKRKKD HFRNDTNTQS FYREKWIYVH KESTKERHGY CTLGEAFNRL DFSSAIQDIR 130 140 150 160 170 180 RFNYVVKLLQ LIAKSQLTSL SGVAQKNYFN ILDKIVQKVL DDHHNPRLIK DLLQDLSSTL 190 200 210 220 230 240 CILIRGVGKS VLVGNINIWI CRLETILAWQ QQLQDLQMTK QVNNGLTLSD LPLHMLNNIL 250 260 270 280 290 300 YRFSDGWDII TLGQVTPTLY MLSEDRQLWK KLCQYHFAEK QFCRHLILSE KGHIEWKLMY 310 320 330 340 350 FALQKHYPAK EQYGDTLHFC RHCSILFWKD SGHPCTAADP DSCFTPVSPQ HFIDLFKF
	Isoform 3 [UniParc]. Checksum: 4222DEAEA61A7487	FASTA	291	34,067
	10 20 30 40 50 60 MEKYSIMKSM NMHRKKGKRT ILEMTQILKR HGYCTLGEAF NRLDFSSAIQ DIRRFNYVVK 70 80 90 100 110 120 LLQLIAKSQL TSLSGVAQKN YFNILDKIVQ KVLDDHHNPR LIKDLLQDLS STLCILIRGV 130 140 150 160 170 180 GKSVLVGNIN IWICRLETIL AWQQQLQDLQ MTKQVNNGLT LSDLPLHMLN NILYRFSDGW 190 200 210 220 230 240 DIITLGQVTP TLYMLSEDRQ LWKKLCQYHF AEKQFCRHLI LSEKGHIEWK LMYFALQKHY 250 260 270 280 290 PAKEQYGDTL HFCRHCSILF WKDSGHPCTA ADPDSCFTPV SPQHFIDLFK F

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression of Fbx25-containing protein in prostate cancer tissues." Tsujiawa K., Mitsui E., Ono Y., Sakamoto K., Konishi N., Yamamoto H. Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Prostatic carcinoma.
[2]	Guo J.H., She X.Y., Yu L. Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3), VARIANT HIS-38. Tissue: Brain and Placenta.
[4]	"Identification of a family of human F-box proteins." Cenciarelli C., Chiaur D.S., Guardavaccaro D., Parks W., Vidal M., Pagano M. Curr. Biol. 9:1177-1179(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 87-367 (ISOFORM 2).
[5]	"Identification of FBXO25-interacting proteins using an integrated proteomics approach." Teixeira F.R., Yokoo S., Gartner C.A., Manfiolli A.O., Baqui M.M., Assmann E.M., Maragno A.L., Yu H., de Lanerolle P., Kobarg J., Gygi S.P., Gomes M.D. Proteomics 10:2746-2757(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 110-120; 135-146; 147-154; 190-202 AND 339-366, INTERACTION WITH BETA-ACTIN AND SKP1, IDENTIFICATION IN A SCF PROTEIN LIGASE COMPLEX, MASS SPECTROMETRY.
[6]	"Characterization of FBX25, encoding a novel brain-expressed F-box protein." Hagens O., Minina E., Schweiger S., Ropers H.-H., Kalscheuer V. Biochim. Biophys. Acta 1760:110-118(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SKP1 AND CUL1, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, MUTAGENESIS OF SER-244.
[7]	"Disruptions of the novel KIAA1202 gene are associated with X-linked mental retardation." Hagens O., Dubos A., Abidi F., Barbi G., Van Zutven L., Hoeltzenbein M., Tommerup N., Moraine C., Fryns J.-P., Chelly J., van Bokhoven H., Gecz J., Dollfus H., Ropers H.-H., Schwartz C.E., de Cassia Stocco Dos Santos R., Kalscheuer V., Hanauer A. Hum. Genet. 118:578-590(2006) [PubMed] [Europe PMC] [Abstract] Cited for: CHROMOSOMAL TRANSLOCATION WITH SHROOM4.
[8]	"FBXO25-associated nuclear domains: a novel subnuclear structure." Manfiolli A.O., Maragno A.L., Baqui M.M., Yokoo S., Teixeira F.R., Oliveira E.B., Gomes M.D. Mol. Biol. Cell 19:1848-1861(2008) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB070270 mRNA. Translation: BAB85128.1. AF419858 mRNA. Translation: AAP97293.1. BC020249 mRNA. Translation: AAH20249.1. BC050393 mRNA. Translation: AAH50393.1. AF174605 mRNA. Translation: AAF04526.1.
IPI	IPI00073356. IPI00289939. IPI00374099.
RefSeq	NP_036305.2. NM_012173.3. NP_904356.1. NM_183420.1. NP_904357.1. NM_183421.1.
UniGene	Hs.438454.
3D structure databases
ProteinModelPortal	Q8TCJ0.
SMR	Q8TCJ0. Positions 228-306.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8TCJ0. 7 interactions.
STRING	9606.ENSP00000276326.
PTM databases
PhosphoSite	Q8TCJ0.
Polymorphism databases
DMDM	61252584.
Proteomic databases
PaxDb	Q8TCJ0.
PRIDE	Q8TCJ0.
Protocols and materials databases
DNASU	26260.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000276326; ENSP00000276326; ENSG00000147364. ENST00000350302; ENSP00000342077; ENSG00000147364. ENST00000352684; ENSP00000341345; ENSG00000147364. ENST00000382824; ENSP00000372274; ENSG00000147364.
GeneID	26260.
KEGG	hsa:26260.
UCSC	uc003wox.3. human. uc003woy.3. human. uc003woz.3. human.
Organism-specific databases
CTD	26260.
GeneCards	GC08P000346.
H-InvDB	HIX0057066.
HGNC	HGNC:13596. FBXO25.
MIM	609098. gene.
neXtProt	NX_Q8TCJ0.
PharmGKB	PA28038.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG327896.
HOGENOM	HOG000263478.
HOVERGEN	HBG051572.
InParanoid	Q8TCJ0.
KO	K10305.
OMA	FALQKYY.
PhylomeDB	Q8TCJ0.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
ArrayExpress	Q8TCJ0.
Bgee	Q8TCJ0.
CleanEx	HS_FBXO25.
Genevestigator	Q8TCJ0.
GermOnline	ENSG00000147364. Homo sapiens.
Family and domain databases
InterPro	IPR001810. F-box_dom_cyclin-like. [Graphical view]
SUPFAM	SSF81383. F-box_dom_Skp2-like. 1 hit.
PROSITE	PS50181. FBOX. False negative. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	26260.
NextBio	48509.
SOURCE	Search...

Entry information

Entry name

FBX25_HUMAN

Accession

Primary (citable) accession number: Q8TCJ0
Secondary accession number(s): Q6PJ83, Q7Z4V4, Q9UKB8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 9, 2003
Last sequence update:	March 15, 2005
Last modified:	May 1, 2013
This is version 93 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.