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Q8TCJ0

- FBX25_HUMAN

UniProt

Q8TCJ0 - FBX25_HUMAN

Protein

F-box only protein 25

Gene

FBXO25

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 3 (15 Mar 2005)
      Previous versions | rss
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    Functioni

    Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. May play a role in accumulation of expanded polyglutamine (polyQ) protein huntingtin (HTT) By similarity.By similarity

    Pathwayi

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. ubiquitin-protein transferase activity Source: UniProtKB

    GO - Biological processi

    1. protein ubiquitination Source: UniProtKB

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    Actin-binding

    Enzyme and pathway databases

    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    F-box only protein 25
    Gene namesi
    Name:FBXO25
    Synonyms:FBX25
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

    Organism-specific databases

    HGNCiHGNC:13596. FBXO25.

    Subcellular locationi

    Nucleus 2 Publications
    Note: In the nucleus, associates with a subnuclear dot-like structure. Colocalized with SKP1.

    GO - Cellular componenti

    1. nucleus Source: UniProtKB
    2. SCF ubiquitin ligase complex Source: UniProtKB
    3. ubiquitin ligase complex Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Involvement in diseasei

    A chromosomal aberration involving FBXO25 is a cause of X-linked mental retardation (XLMR). Translocation t(X;8)(p11.22;p23.3) with SHROOM4.

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi244 – 2441S → L: Loss of SKP1-binding. 1 Publication

    Organism-specific databases

    PharmGKBiPA28038.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 367367F-box only protein 25PRO_0000119911Add
    BLAST

    Proteomic databases

    MaxQBiQ8TCJ0.
    PaxDbiQ8TCJ0.
    PRIDEiQ8TCJ0.

    PTM databases

    PhosphoSiteiQ8TCJ0.

    Expressioni

    Tissue specificityi

    Expressed in all brain tissue observed.1 Publication

    Gene expression databases

    ArrayExpressiQ8TCJ0.
    BgeeiQ8TCJ0.
    CleanExiHS_FBXO25.
    GenevestigatoriQ8TCJ0.

    Interactioni

    Subunit structurei

    Part of a SCF (SKP1-cullin-F-box) protein ligase complex consisting of FBXO25, SKP1, CUL1 and RBX1. Interacts directly with SKP1 and CUL1. Interacts (via C-terminus) with beta-actin (via N-terminus).2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ACTBP607093EBI-6264551,EBI-353944

    Protein-protein interaction databases

    BioGridi117646. 190 interactions.
    IntActiQ8TCJ0. 12 interactions.
    STRINGi9606.ENSP00000276326.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8TCJ0.
    SMRiQ8TCJ0. Positions 228-305.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini226 – 27449F-boxAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 8383Interaction with beta-actinAdd
    BLAST

    Domaini

    The F-box is necessary for the interaction with SKP1.By similarity

    Sequence similaritiesi

    Contains 1 F-box domain.Curated

    Phylogenomic databases

    eggNOGiNOG327896.
    HOGENOMiHOG000263478.
    HOVERGENiHBG051572.
    InParanoidiQ8TCJ0.
    KOiK10305.
    OMAiDWKLMYF.
    PhylomeDBiQ8TCJ0.
    TreeFamiTF313070.

    Family and domain databases

    InterProiIPR001810. F-box_dom.
    [Graphical view]
    SUPFAMiSSF81383. SSF81383. 1 hit.
    ProtoNetiSearch...

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8TCJ0-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MPFLGQDWRS PGWSWIKTED GWKRCESCSQ KLERENNRCN ISHSIILNSE    50
    DGEIFNNEEH EYASKKRKKD HFRNDTNTQS FYREKWIYVH KESTKERHGY 100
    CTLGEAFNRL DFSSAIQDIR RFNYVVKLLQ LIAKSQLTSL SGVAQKNYFN 150
    ILDKIVQKVL DDHHNPRLIK DLLQDLSSTL CILIRGVGKS VLVGNINIWI 200
    CRLETILAWQ QQLQDLQMTK QVNNGLTLSD LPLHMLNNIL YRFSDGWDII 250
    TLGQVTPTLY MLSEDRQLWK KLCQYHFAEK QFCRHLILSE KGHIEWKLMY 300
    FALQKHYPAK EQYGDTLHFC RHCSILFWKD YHLALLFKDS GHPCTAADPD 350
    SCFTPVSPQH FIDLFKF 367

    Note: No experimental confirmation available.

    Length:367
    Mass (Da):43,313
    Last modified:March 15, 2005 - v3
    Checksum:i1C1C6AD43A92754F
    GO
    Isoform 2 (identifier: Q8TCJ0-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         331-339: Missing.

    Show »
    Length:358
    Mass (Da):42,211
    Checksum:i11CCEFCA20F3BF37
    GO
    Isoform 3 (identifier: Q8TCJ0-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-67: Missing.
         68-96: KKDHFRNDTNTQSFYREKWIYVHKESTKE → MEKYSIMKSMNMHRKKGKRTILEMTQILK
         331-339: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:291
    Mass (Da):34,067
    Checksum:i4222DEAEA61A7487
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti36 – 361N → D.
    Corresponds to variant rs17665340 [ dbSNP | Ensembl ].
    VAR_049043
    Natural varianti38 – 381R → H.1 Publication
    Corresponds to variant rs10090550 [ dbSNP | Ensembl ].
    VAR_061167

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 6767Missing in isoform 3. 1 PublicationVSP_013060Add
    BLAST
    Alternative sequencei68 – 9629KKDHF…ESTKE → MEKYSIMKSMNMHRKKGKRT ILEMTQILK in isoform 3. 1 PublicationVSP_013061Add
    BLAST
    Alternative sequencei331 – 3399Missing in isoform 2 and isoform 3. 4 PublicationsVSP_007374

    Sequence conflict

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti87 – 9610IYVHKESTKE → LILTSVLLFQ in AAF04526. (PubMed:10531035)Curated
    Sequence conflicti121 – 1211R → T in AAF04526. (PubMed:10531035)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB070270 mRNA. Translation: BAB85128.1.
    AF419858 mRNA. Translation: AAP97293.1.
    BC020249 mRNA. Translation: AAH20249.1.
    BC050393 mRNA. Translation: AAH50393.1.
    AF174605 mRNA. Translation: AAF04526.1.
    CCDSiCCDS5952.1. [Q8TCJ0-2]
    CCDS5953.1. [Q8TCJ0-1]
    CCDS5954.1. [Q8TCJ0-3]
    RefSeqiNP_036305.2. NM_012173.3. [Q8TCJ0-3]
    NP_904356.1. NM_183420.1. [Q8TCJ0-2]
    NP_904357.1. NM_183421.1. [Q8TCJ0-1]
    UniGeneiHs.438454.

    Genome annotation databases

    EnsembliENST00000276326; ENSP00000276326; ENSG00000147364. [Q8TCJ0-1]
    ENST00000350302; ENSP00000342077; ENSG00000147364. [Q8TCJ0-2]
    ENST00000352684; ENSP00000341345; ENSG00000147364. [Q8TCJ0-3]
    ENST00000382824; ENSP00000372274; ENSG00000147364. [Q8TCJ0-3]
    GeneIDi26260.
    KEGGihsa:26260.
    UCSCiuc003wox.3. human. [Q8TCJ0-1]
    uc003woy.3. human. [Q8TCJ0-2]
    uc003woz.3. human. [Q8TCJ0-3]

    Keywords - Coding sequence diversityi

    Alternative splicing, Chromosomal rearrangement, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB070270 mRNA. Translation: BAB85128.1 .
    AF419858 mRNA. Translation: AAP97293.1 .
    BC020249 mRNA. Translation: AAH20249.1 .
    BC050393 mRNA. Translation: AAH50393.1 .
    AF174605 mRNA. Translation: AAF04526.1 .
    CCDSi CCDS5952.1. [Q8TCJ0-2 ]
    CCDS5953.1. [Q8TCJ0-1 ]
    CCDS5954.1. [Q8TCJ0-3 ]
    RefSeqi NP_036305.2. NM_012173.3. [Q8TCJ0-3 ]
    NP_904356.1. NM_183420.1. [Q8TCJ0-2 ]
    NP_904357.1. NM_183421.1. [Q8TCJ0-1 ]
    UniGenei Hs.438454.

    3D structure databases

    ProteinModelPortali Q8TCJ0.
    SMRi Q8TCJ0. Positions 228-305.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 117646. 190 interactions.
    IntActi Q8TCJ0. 12 interactions.
    STRINGi 9606.ENSP00000276326.

    PTM databases

    PhosphoSitei Q8TCJ0.

    Proteomic databases

    MaxQBi Q8TCJ0.
    PaxDbi Q8TCJ0.
    PRIDEi Q8TCJ0.

    Protocols and materials databases

    DNASUi 26260.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000276326 ; ENSP00000276326 ; ENSG00000147364 . [Q8TCJ0-1 ]
    ENST00000350302 ; ENSP00000342077 ; ENSG00000147364 . [Q8TCJ0-2 ]
    ENST00000352684 ; ENSP00000341345 ; ENSG00000147364 . [Q8TCJ0-3 ]
    ENST00000382824 ; ENSP00000372274 ; ENSG00000147364 . [Q8TCJ0-3 ]
    GeneIDi 26260.
    KEGGi hsa:26260.
    UCSCi uc003wox.3. human. [Q8TCJ0-1 ]
    uc003woy.3. human. [Q8TCJ0-2 ]
    uc003woz.3. human. [Q8TCJ0-3 ]

    Organism-specific databases

    CTDi 26260.
    GeneCardsi GC08P000346.
    H-InvDB HIX0057066.
    HGNCi HGNC:13596. FBXO25.
    MIMi 609098. gene.
    neXtProti NX_Q8TCJ0.
    PharmGKBi PA28038.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG327896.
    HOGENOMi HOG000263478.
    HOVERGENi HBG051572.
    InParanoidi Q8TCJ0.
    KOi K10305.
    OMAi DWKLMYF.
    PhylomeDBi Q8TCJ0.
    TreeFami TF313070.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .

    Miscellaneous databases

    GenomeRNAii 26260.
    NextBioi 48509.
    PROi Q8TCJ0.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8TCJ0.
    Bgeei Q8TCJ0.
    CleanExi HS_FBXO25.
    Genevestigatori Q8TCJ0.

    Family and domain databases

    InterProi IPR001810. F-box_dom.
    [Graphical view ]
    SUPFAMi SSF81383. SSF81383. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Expression of Fbx25-containing protein in prostate cancer tissues."
      Tsujiawa K., Mitsui E., Ono Y., Sakamoto K., Konishi N., Yamamoto H.
      Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
      Tissue: Prostatic carcinoma.
    2. Guo J.H., She X.Y., Yu L.
      Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3), VARIANT HIS-38.
      Tissue: Brain and Placenta.
    4. Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 87-367 (ISOFORM 2).
    5. Cited for: PROTEIN SEQUENCE OF 110-120; 135-146; 147-154; 190-202 AND 339-366, INTERACTION WITH BETA-ACTIN AND SKP1, IDENTIFICATION IN A SCF PROTEIN LIGASE COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    6. "Characterization of FBX25, encoding a novel brain-expressed F-box protein."
      Hagens O., Minina E., Schweiger S., Ropers H.-H., Kalscheuer V.
      Biochim. Biophys. Acta 1760:110-118(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SKP1 AND CUL1, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, MUTAGENESIS OF SER-244.
    7. Cited for: CHROMOSOMAL TRANSLOCATION WITH SHROOM4.
    8. Cited for: SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiFBX25_HUMAN
    AccessioniPrimary (citable) accession number: Q8TCJ0
    Secondary accession number(s): Q6PJ83, Q7Z4V4, Q9UKB8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 9, 2003
    Last sequence update: March 15, 2005
    Last modified: October 1, 2014
    This is version 105 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 8
      Human chromosome 8: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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