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Protein

Phosphatidylinositol 4-kinase type 2-beta

Gene

PI4K2B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Together with PI4K2A and the type III PI4Ks (PIK4CA and PIK4CB) it contributes to the overall PI4-kinase activity of the cell. This contribution may be especially significant in plasma membrane, endosomal and Golgi compartments. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). Contributes to the production of InsP3 in stimulated cells and is likely to be involved in the regulation of vesicular trafficking.1 Publication

Catalytic activityi

ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate.

Enzyme regulationi

Recruited and activated by membrane association. Binding to the microsomal membrane has been shown to enhance its activity.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei148ATPBy similarity1
Binding sitei344ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi127 – 133ATPBy similarity7
Nucleotide bindingi257 – 260ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS00529-MONOMER.
ZFISH:HS00529-MONOMER.
BRENDAi2.7.1.67. 2681.
ReactomeiR-HSA-1483248. Synthesis of PIPs at the ER membrane.
R-HSA-1660499. Synthesis of PIPs at the plasma membrane.
R-HSA-1660514. Synthesis of PIPs at the Golgi membrane.
R-HSA-1660516. Synthesis of PIPs at the early endosome membrane.
SignaLinkiQ8TCG2.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 4-kinase type 2-beta (EC:2.7.1.67)
Alternative name(s):
Phosphatidylinositol 4-kinase type II-beta
Short name:
PI4KII-BETA
Gene namesi
Name:PI4K2B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:18215. PI4K2B.

Subcellular locationi

  • Cytoplasm
  • Membrane

  • Note: Mostly cytoplasmic but also found associated with the plasma membrane, the Golgi and endosomes. Compared to PI4K2A, a larger fraction of PI4K2B is cytosolic due to a smaller extent of palmitoylation. Translocates to membranes where it is recruited by PDGF stimulation by a Rac-GTP-dependent mechanism.

GO - Cellular componenti

  • cytoplasm Source: HPA
  • cytosol Source: Reactome
  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi55300.
OpenTargetsiENSG00000038210.
PharmGKBiPA142671173.

Chemistry databases

ChEMBLiCHEMBL2096619.
GuidetoPHARMACOLOGYi2499.

Polymorphism and mutation databases

BioMutaiPI4K2B.
DMDMi74715788.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002851641 – 481Phosphatidylinositol 4-kinase type 2-betaAdd BLAST481

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei12PhosphoserineCombined sources1
Modified residuei17PhosphoserineCombined sources1
Modified residuei45PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8TCG2.
MaxQBiQ8TCG2.
PaxDbiQ8TCG2.
PeptideAtlasiQ8TCG2.
PRIDEiQ8TCG2.

PTM databases

iPTMnetiQ8TCG2.
PhosphoSitePlusiQ8TCG2.
SwissPalmiQ8TCG2.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiENSG00000038210.
CleanExiHS_PI4K2B.
GenevisibleiQ8TCG2. HS.

Organism-specific databases

HPAiHPA004099.

Interactioni

Protein-protein interaction databases

BioGridi120587. 17 interactors.
IntActiQ8TCG2. 1 interactor.
STRINGi9606.ENSP00000264864.

Structurei

Secondary structure

1481
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi104 – 118Combined sources15
Beta strandi124 – 130Combined sources7
Beta strandi134 – 137Combined sources4
Beta strandi143 – 149Combined sources7
Helixi185 – 199Combined sources15
Beta strandi207 – 212Combined sources6
Beta strandi252 – 258Combined sources7
Helixi266 – 272Combined sources7
Turni273 – 275Combined sources3
Helixi280 – 299Combined sources20
Beta strandi307 – 314Combined sources8
Beta strandi337 – 342Combined sources6
Beta strandi356 – 358Combined sources3
Helixi363 – 366Combined sources4
Helixi368 – 371Combined sources4
Helixi376 – 386Combined sources11
Helixi389 – 403Combined sources15
Helixi411 – 433Combined sources23
Helixi438 – 441Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4WTVX-ray1.90A/B90-165[»]
A/B176-450[»]
ProteinModelPortaliQ8TCG2.
SMRiQ8TCG2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini129 – 436PI3K/PI4KAdd BLAST308

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni153 – 155Important for substrate bindingBy similarity3
Regioni161 – 174Important for interaction with membranesBy similarityAdd BLAST14
Regioni264 – 272Important for interaction with membranesBy similarity9
Regioni357 – 366Important for interaction with membranesBy similarity10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi19 – 22Poly-Glu4

Sequence similaritiesi

Contains 1 PI3K/PI4K domain.Curated

Phylogenomic databases

eggNOGiKOG2381. Eukaryota.
ENOG410XP06. LUCA.
GeneTreeiENSGT00390000010434.
HOGENOMiHOG000294076.
HOVERGENiHBG059542.
InParanoidiQ8TCG2.
KOiK13711.
OMAiCEDLHEL.
OrthoDBiEOG091G09F3.
PhylomeDBiQ8TCG2.
TreeFamiTF314740.

Family and domain databases

InterProiIPR000403. PI3/4_kinase_cat_dom.
[Graphical view]
PfamiPF00454. PI3_PI4_kinase. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8TCG2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDPSEPDRL ASADGGSPEE EEDGEREPLL PRIAWAHPRR GAPGSAVRLL
60 70 80 90 100
DAAGEEGEAG DEELPLPPGD VGVSRSSSAE LDRSRPAVSV TIGTSEMNAF
110 120 130 140 150
LDDPEFADIM LRAEQAIEVG IFPERISQGS SGSYFVKDPK RKIIGVFKPK
160 170 180 190 200
SEEPYGQLNP KWTKYVHKVC CPCCFGRGCL IPNQGYLSEA GAYLVDNKLH
210 220 230 240 250
LSIVPKTKVV WLVSETFNYN AIDRAKSRGK KYALEKVPKV GRKFHRIGLP
260 270 280 290 300
PKIGSFQLFV EGYKEAEYWL RKFEADPLPE NIRKQFQSQF ERLVILDYII
310 320 330 340 350
RNTDRGNDNW LVRYEKQKCE KEIDHKESKW IDDEEFLIKI AAIDNGLAFP
360 370 380 390 400
FKHPDEWRAY PFHWAWLPQA KVPFSEEIRN LILPYISDMN FVQDLCEDLY
410 420 430 440 450
ELFKTDKGFD KATFESQMSV MRGQILNLTQ ALRDGKSPFQ LVQIPCVIVE
460 470 480
RSQGGSQGRI VHLSNSFTQT VNCRKPFFSS W
Length:481
Mass (Da):54,744
Last modified:June 1, 2002 - v1
Checksum:iB4E16257E0AEC026
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03197478S → P.Combined sources4 PublicationsCorresponds to variant rs313549dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY065990 mRNA. Translation: AAL47580.1.
AF411320 mRNA. Translation: AAL04154.1.
AY091514 mRNA. Translation: AAM12049.1.
AK001967 mRNA. Translation: BAA92006.1.
BC051749 mRNA. Translation: AAH51749.1.
CCDSiCCDS3433.1.
RefSeqiNP_060793.2. NM_018323.3.
UniGeneiHs.191701.

Genome annotation databases

EnsembliENST00000264864; ENSP00000264864; ENSG00000038210.
GeneIDi55300.
KEGGihsa:55300.
UCSCiuc003grk.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY065990 mRNA. Translation: AAL47580.1.
AF411320 mRNA. Translation: AAL04154.1.
AY091514 mRNA. Translation: AAM12049.1.
AK001967 mRNA. Translation: BAA92006.1.
BC051749 mRNA. Translation: AAH51749.1.
CCDSiCCDS3433.1.
RefSeqiNP_060793.2. NM_018323.3.
UniGeneiHs.191701.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4WTVX-ray1.90A/B90-165[»]
A/B176-450[»]
ProteinModelPortaliQ8TCG2.
SMRiQ8TCG2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120587. 17 interactors.
IntActiQ8TCG2. 1 interactor.
STRINGi9606.ENSP00000264864.

Chemistry databases

ChEMBLiCHEMBL2096619.
GuidetoPHARMACOLOGYi2499.

PTM databases

iPTMnetiQ8TCG2.
PhosphoSitePlusiQ8TCG2.
SwissPalmiQ8TCG2.

Polymorphism and mutation databases

BioMutaiPI4K2B.
DMDMi74715788.

Proteomic databases

EPDiQ8TCG2.
MaxQBiQ8TCG2.
PaxDbiQ8TCG2.
PeptideAtlasiQ8TCG2.
PRIDEiQ8TCG2.

Protocols and materials databases

DNASUi55300.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000264864; ENSP00000264864; ENSG00000038210.
GeneIDi55300.
KEGGihsa:55300.
UCSCiuc003grk.3. human.

Organism-specific databases

CTDi55300.
DisGeNETi55300.
GeneCardsiPI4K2B.
HGNCiHGNC:18215. PI4K2B.
HPAiHPA004099.
MIMi612101. gene.
neXtProtiNX_Q8TCG2.
OpenTargetsiENSG00000038210.
PharmGKBiPA142671173.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2381. Eukaryota.
ENOG410XP06. LUCA.
GeneTreeiENSGT00390000010434.
HOGENOMiHOG000294076.
HOVERGENiHBG059542.
InParanoidiQ8TCG2.
KOiK13711.
OMAiCEDLHEL.
OrthoDBiEOG091G09F3.
PhylomeDBiQ8TCG2.
TreeFamiTF314740.

Enzyme and pathway databases

BioCyciMetaCyc:HS00529-MONOMER.
ZFISH:HS00529-MONOMER.
BRENDAi2.7.1.67. 2681.
ReactomeiR-HSA-1483248. Synthesis of PIPs at the ER membrane.
R-HSA-1660499. Synthesis of PIPs at the plasma membrane.
R-HSA-1660514. Synthesis of PIPs at the Golgi membrane.
R-HSA-1660516. Synthesis of PIPs at the early endosome membrane.
SignaLinkiQ8TCG2.

Miscellaneous databases

ChiTaRSiPI4K2B. human.
GeneWikiiPI4K2B.
GenomeRNAii55300.
PROiQ8TCG2.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000038210.
CleanExiHS_PI4K2B.
GenevisibleiQ8TCG2. HS.

Family and domain databases

InterProiIPR000403. PI3/4_kinase_cat_dom.
[Graphical view]
PfamiPF00454. PI3_PI4_kinase. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiP4K2B_HUMAN
AccessioniPrimary (citable) accession number: Q8TCG2
Secondary accession number(s): Q9NUW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: June 1, 2002
Last modified: November 2, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.