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Q8TCG2 (P4K2B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphatidylinositol 4-kinase type 2-beta

EC=2.7.1.67
Alternative name(s):
Phosphatidylinositol 4-kinase type II-beta
Short name=PI4KII-BETA
Gene names
Name:PI4K2B
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length481 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Together with PI4K2A and the type III PI4Ks (PIK4CA and PIK4CB) it contributes to the overall PI4-kinase activity of the cell. This contribution may be especially significant in plasma membrane, endosomal and Golgi compartments. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). Contributes to the production of InsP3 in stimulated cells and is likely to be involved in the regulation of vesicular trafficking. Ref.1

Catalytic activity

ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate.

Enzyme regulation

Recruited and activated by membrane association. Binding to the microsomal membrane has been shown to enhance its activity. Ref.2

Subcellular location

Cytoplasm. Membrane. Note: Mostly cytoplasmic but also found associated with the plasma membrane, the Golgi and endosomes. Compared to PI4K2A, a larger fraction of PI4K2B is cytosolic due to a smaller extent of palmitoylation. Translocates to membranes where it is recruited by PDGF stimulation by a Rac-GTP-dependent mechanism. Ref.1 Ref.2

Tissue specificity

Widely expressed. Ref.1

Sequence similarities

Belongs to the PI3/PI4-kinase family. Type II PI4K subfamily.

Contains 1 PI3K/PI4K domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 481481Phosphatidylinositol 4-kinase type 2-beta
PRO_0000285164

Regions

Domain129 – 436308PI3K/PI4K
Compositional bias19 – 224Poly-Glu

Amino acid modifications

Modified residue121Phosphoserine Ref.6
Modified residue171Phosphoserine Ref.6

Natural variations

Natural variant781S → P. Ref.1 Ref.2 Ref.4 Ref.5 Ref.7
Corresponds to variant rs313549 [ dbSNP | Ensembl ].
VAR_031974

Sequences

Sequence LengthMass (Da)Tools
Q8TCG2 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: B4E16257E0AEC026

FASTA48154,744
        10         20         30         40         50         60 
MEDPSEPDRL ASADGGSPEE EEDGEREPLL PRIAWAHPRR GAPGSAVRLL DAAGEEGEAG 

        70         80         90        100        110        120 
DEELPLPPGD VGVSRSSSAE LDRSRPAVSV TIGTSEMNAF LDDPEFADIM LRAEQAIEVG 

       130        140        150        160        170        180 
IFPERISQGS SGSYFVKDPK RKIIGVFKPK SEEPYGQLNP KWTKYVHKVC CPCCFGRGCL 

       190        200        210        220        230        240 
IPNQGYLSEA GAYLVDNKLH LSIVPKTKVV WLVSETFNYN AIDRAKSRGK KYALEKVPKV 

       250        260        270        280        290        300 
GRKFHRIGLP PKIGSFQLFV EGYKEAEYWL RKFEADPLPE NIRKQFQSQF ERLVILDYII 

       310        320        330        340        350        360 
RNTDRGNDNW LVRYEKQKCE KEIDHKESKW IDDEEFLIKI AAIDNGLAFP FKHPDEWRAY 

       370        380        390        400        410        420 
PFHWAWLPQA KVPFSEEIRN LILPYISDMN FVQDLCEDLY ELFKTDKGFD KATFESQMSV 

       430        440        450        460        470        480 
MRGQILNLTQ ALRDGKSPFQ LVQIPCVIVE RSQGGSQGRI VHLSNSFTQT VNCRKPFFSS 


W 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of type II phosphatidylinositol 4-kinase isoforms reveals association of the enzymes with endosomal vesicular compartments."
Balla A., Tuymetova G., Barshishat M., Geiszt M., Balla T.
J. Biol. Chem. 277:20041-20050(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, FUNCTION, SUBCELLULAR LOCATION, VARIANT PRO-78.
[2]"Type II phosphatidylinositol 4-kinase beta is a cytosolic and peripheral membrane protein that is recruited to the plasma membrane and activated by Rac-GTP."
Wei Y.J., Sun H.Q., Yamamoto M., Wlodarski P., Kunii K., Martinez M., Barylko B., Albanesi J.P., Yin H.L.
J. Biol. Chem. 277:46586-46593(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, ENZYME REGULATION, VARIANT PRO-78.
Tissue: Brain.
[3]"Beta isoform of human type II phosphatidylinositol 4-kinase (PI4KIIbeta)."
Hong W.
Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PRO-78.
Tissue: Placenta.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PRO-78.
Tissue: Skin.
[6]"Large-scale proteomics analysis of the human kinome."
Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H.
Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-12 AND SER-17, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] PRO-78, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY065990 mRNA. Translation: AAL47580.1.
AF411320 mRNA. Translation: AAL04154.1.
AY091514 mRNA. Translation: AAM12049.1.
AK001967 mRNA. Translation: BAA92006.1.
BC051749 mRNA. Translation: AAH51749.1.
CCDSCCDS3433.1.
RefSeqNP_060793.2. NM_018323.3.
UniGeneHs.191701.

3D structure databases

ProteinModelPortalQ8TCG2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120587. 6 interactions.
IntActQ8TCG2. 1 interaction.
STRING9606.ENSP00000264864.

Chemistry

BindingDBQ8TCG2.
ChEMBLCHEMBL2096619.
GuidetoPHARMACOLOGY2499.

PTM databases

PhosphoSiteQ8TCG2.

Polymorphism databases

DMDM74715788.

Proteomic databases

MaxQBQ8TCG2.
PaxDbQ8TCG2.
PRIDEQ8TCG2.

Protocols and materials databases

DNASU55300.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000264864; ENSP00000264864; ENSG00000038210.
GeneID55300.
KEGGhsa:55300.
UCSCuc003grk.2. human.

Organism-specific databases

CTD55300.
GeneCardsGC04P025163.
HGNCHGNC:18215. PI4K2B.
HPAHPA004099.
MIM612101. gene.
neXtProtNX_Q8TCG2.
PharmGKBPA142671173.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG287949.
HOGENOMHOG000294076.
HOVERGENHBG059542.
InParanoidQ8TCG2.
KOK13711.
OMAPRIAWAH.
PhylomeDBQ8TCG2.
TreeFamTF314740.

Enzyme and pathway databases

BioCycMetaCyc:HS00529-MONOMER.
ReactomeREACT_111217. Metabolism.
SignaLinkQ8TCG2.

Gene expression databases

ArrayExpressQ8TCG2.
BgeeQ8TCG2.
CleanExHS_PI4K2B.
GenevestigatorQ8TCG2.

Family and domain databases

InterProIPR000403. PI3/4_kinase_cat_dom.
[Graphical view]
PfamPF00454. PI3_PI4_kinase. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiPI4K2B.
GenomeRNAi55300.
NextBio59520.
PROQ8TCG2.
SOURCESearch...

Entry information

Entry nameP4K2B_HUMAN
AccessionPrimary (citable) accession number: Q8TCG2
Secondary accession number(s): Q9NUW2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: June 1, 2002
Last modified: July 9, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM