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Q8TCG2

- P4K2B_HUMAN

UniProt

Q8TCG2 - P4K2B_HUMAN

Protein

Phosphatidylinositol 4-kinase type 2-beta

Gene

PI4K2B

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Together with PI4K2A and the type III PI4Ks (PIK4CA and PIK4CB) it contributes to the overall PI4-kinase activity of the cell. This contribution may be especially significant in plasma membrane, endosomal and Golgi compartments. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). Contributes to the production of InsP3 in stimulated cells and is likely to be involved in the regulation of vesicular trafficking.1 Publication

    Catalytic activityi

    ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate.

    Enzyme regulationi

    Recruited and activated by membrane association. Binding to the microsomal membrane has been shown to enhance its activity.1 Publication

    GO - Molecular functioni

    1. 1-phosphatidylinositol 4-kinase activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. phosphatidylinositol biosynthetic process Source: Reactome
    2. phospholipid metabolic process Source: Reactome
    3. small molecule metabolic process Source: Reactome

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:HS00529-MONOMER.
    ReactomeiREACT_120756. Synthesis of PIPs at the early endosome membrane.
    REACT_120836. Synthesis of PIPs at the Golgi membrane.
    REACT_121025. Synthesis of PIPs at the plasma membrane.
    REACT_121168. Synthesis of PIPs at the ER membrane.
    SignaLinkiQ8TCG2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphatidylinositol 4-kinase type 2-beta (EC:2.7.1.67)
    Alternative name(s):
    Phosphatidylinositol 4-kinase type II-beta
    Short name:
    PI4KII-BETA
    Gene namesi
    Name:PI4K2B
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 4

    Organism-specific databases

    HGNCiHGNC:18215. PI4K2B.

    Subcellular locationi

    Cytoplasm. Membrane
    Note: Mostly cytoplasmic but also found associated with the plasma membrane, the Golgi and endosomes. Compared to PI4K2A, a larger fraction of PI4K2B is cytosolic due to a smaller extent of palmitoylation. Translocates to membranes where it is recruited by PDGF stimulation by a Rac-GTP-dependent mechanism.

    GO - Cellular componenti

    1. cytoplasm Source: HPA
    2. cytosol Source: Reactome
    3. membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA142671173.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 481481Phosphatidylinositol 4-kinase type 2-betaPRO_0000285164Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei12 – 121Phosphoserine1 Publication
    Modified residuei17 – 171Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ8TCG2.
    PaxDbiQ8TCG2.
    PRIDEiQ8TCG2.

    PTM databases

    PhosphoSiteiQ8TCG2.

    Expressioni

    Tissue specificityi

    Widely expressed.1 Publication

    Gene expression databases

    ArrayExpressiQ8TCG2.
    BgeeiQ8TCG2.
    CleanExiHS_PI4K2B.
    GenevestigatoriQ8TCG2.

    Organism-specific databases

    HPAiHPA004099.

    Interactioni

    Protein-protein interaction databases

    BioGridi120587. 6 interactions.
    IntActiQ8TCG2. 1 interaction.
    STRINGi9606.ENSP00000264864.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8TCG2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini129 – 436308PI3K/PI4KAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi19 – 224Poly-Glu

    Sequence similaritiesi

    Contains 1 PI3K/PI4K domain.Curated

    Phylogenomic databases

    eggNOGiNOG287949.
    HOGENOMiHOG000294076.
    HOVERGENiHBG059542.
    InParanoidiQ8TCG2.
    KOiK13711.
    OMAiPRIAWAH.
    PhylomeDBiQ8TCG2.
    TreeFamiTF314740.

    Family and domain databases

    InterProiIPR000403. PI3/4_kinase_cat_dom.
    [Graphical view]
    PfamiPF00454. PI3_PI4_kinase. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8TCG2-1 [UniParc]FASTAAdd to Basket

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    MEDPSEPDRL ASADGGSPEE EEDGEREPLL PRIAWAHPRR GAPGSAVRLL    50
    DAAGEEGEAG DEELPLPPGD VGVSRSSSAE LDRSRPAVSV TIGTSEMNAF 100
    LDDPEFADIM LRAEQAIEVG IFPERISQGS SGSYFVKDPK RKIIGVFKPK 150
    SEEPYGQLNP KWTKYVHKVC CPCCFGRGCL IPNQGYLSEA GAYLVDNKLH 200
    LSIVPKTKVV WLVSETFNYN AIDRAKSRGK KYALEKVPKV GRKFHRIGLP 250
    PKIGSFQLFV EGYKEAEYWL RKFEADPLPE NIRKQFQSQF ERLVILDYII 300
    RNTDRGNDNW LVRYEKQKCE KEIDHKESKW IDDEEFLIKI AAIDNGLAFP 350
    FKHPDEWRAY PFHWAWLPQA KVPFSEEIRN LILPYISDMN FVQDLCEDLY 400
    ELFKTDKGFD KATFESQMSV MRGQILNLTQ ALRDGKSPFQ LVQIPCVIVE 450
    RSQGGSQGRI VHLSNSFTQT VNCRKPFFSS W 481
    Length:481
    Mass (Da):54,744
    Last modified:June 1, 2002 - v1
    Checksum:iB4E16257E0AEC026
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti78 – 781S → P.5 Publications
    Corresponds to variant rs313549 [ dbSNP | Ensembl ].
    VAR_031974

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY065990 mRNA. Translation: AAL47580.1.
    AF411320 mRNA. Translation: AAL04154.1.
    AY091514 mRNA. Translation: AAM12049.1.
    AK001967 mRNA. Translation: BAA92006.1.
    BC051749 mRNA. Translation: AAH51749.1.
    CCDSiCCDS3433.1.
    RefSeqiNP_060793.2. NM_018323.3.
    UniGeneiHs.191701.

    Genome annotation databases

    EnsembliENST00000264864; ENSP00000264864; ENSG00000038210.
    GeneIDi55300.
    KEGGihsa:55300.
    UCSCiuc003grk.2. human.

    Polymorphism databases

    DMDMi74715788.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY065990 mRNA. Translation: AAL47580.1 .
    AF411320 mRNA. Translation: AAL04154.1 .
    AY091514 mRNA. Translation: AAM12049.1 .
    AK001967 mRNA. Translation: BAA92006.1 .
    BC051749 mRNA. Translation: AAH51749.1 .
    CCDSi CCDS3433.1.
    RefSeqi NP_060793.2. NM_018323.3.
    UniGenei Hs.191701.

    3D structure databases

    ProteinModelPortali Q8TCG2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 120587. 6 interactions.
    IntActi Q8TCG2. 1 interaction.
    STRINGi 9606.ENSP00000264864.

    Chemistry

    BindingDBi Q8TCG2.
    ChEMBLi CHEMBL2096619.
    GuidetoPHARMACOLOGYi 2499.

    PTM databases

    PhosphoSitei Q8TCG2.

    Polymorphism databases

    DMDMi 74715788.

    Proteomic databases

    MaxQBi Q8TCG2.
    PaxDbi Q8TCG2.
    PRIDEi Q8TCG2.

    Protocols and materials databases

    DNASUi 55300.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000264864 ; ENSP00000264864 ; ENSG00000038210 .
    GeneIDi 55300.
    KEGGi hsa:55300.
    UCSCi uc003grk.2. human.

    Organism-specific databases

    CTDi 55300.
    GeneCardsi GC04P025163.
    HGNCi HGNC:18215. PI4K2B.
    HPAi HPA004099.
    MIMi 612101. gene.
    neXtProti NX_Q8TCG2.
    PharmGKBi PA142671173.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG287949.
    HOGENOMi HOG000294076.
    HOVERGENi HBG059542.
    InParanoidi Q8TCG2.
    KOi K13711.
    OMAi PRIAWAH.
    PhylomeDBi Q8TCG2.
    TreeFami TF314740.

    Enzyme and pathway databases

    BioCyci MetaCyc:HS00529-MONOMER.
    Reactomei REACT_120756. Synthesis of PIPs at the early endosome membrane.
    REACT_120836. Synthesis of PIPs at the Golgi membrane.
    REACT_121025. Synthesis of PIPs at the plasma membrane.
    REACT_121168. Synthesis of PIPs at the ER membrane.
    SignaLinki Q8TCG2.

    Miscellaneous databases

    GeneWikii PI4K2B.
    GenomeRNAii 55300.
    NextBioi 59520.
    PROi Q8TCG2.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8TCG2.
    Bgeei Q8TCG2.
    CleanExi HS_PI4K2B.
    Genevestigatori Q8TCG2.

    Family and domain databases

    InterProi IPR000403. PI3/4_kinase_cat_dom.
    [Graphical view ]
    Pfami PF00454. PI3_PI4_kinase. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of type II phosphatidylinositol 4-kinase isoforms reveals association of the enzymes with endosomal vesicular compartments."
      Balla A., Tuymetova G., Barshishat M., Geiszt M., Balla T.
      J. Biol. Chem. 277:20041-20050(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, FUNCTION, SUBCELLULAR LOCATION, VARIANT PRO-78.
    2. "Type II phosphatidylinositol 4-kinase beta is a cytosolic and peripheral membrane protein that is recruited to the plasma membrane and activated by Rac-GTP."
      Wei Y.J., Sun H.Q., Yamamoto M., Wlodarski P., Kunii K., Martinez M., Barylko B., Albanesi J.P., Yin H.L.
      J. Biol. Chem. 277:46586-46593(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, ENZYME REGULATION, VARIANT PRO-78.
      Tissue: Brain.
    3. "Beta isoform of human type II phosphatidylinositol 4-kinase (PI4KIIbeta)."
      Hong W.
      Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PRO-78.
      Tissue: Placenta.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PRO-78.
      Tissue: Skin.
    6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-12 AND SER-17, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANT [LARGE SCALE ANALYSIS] PRO-78, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.

    Entry informationi

    Entry nameiP4K2B_HUMAN
    AccessioniPrimary (citable) accession number: Q8TCG2
    Secondary accession number(s): Q9NUW2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 2007
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 4
      Human chromosome 4: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3