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Q8TCG2

- P4K2B_HUMAN

UniProt

Q8TCG2 - P4K2B_HUMAN

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Protein

Phosphatidylinositol 4-kinase type 2-beta

Gene

PI4K2B

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Together with PI4K2A and the type III PI4Ks (PIK4CA and PIK4CB) it contributes to the overall PI4-kinase activity of the cell. This contribution may be especially significant in plasma membrane, endosomal and Golgi compartments. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). Contributes to the production of InsP3 in stimulated cells and is likely to be involved in the regulation of vesicular trafficking.1 Publication

Catalytic activityi

ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate.

Enzyme regulationi

Recruited and activated by membrane association. Binding to the microsomal membrane has been shown to enhance its activity.1 Publication

GO - Molecular functioni

  1. 1-phosphatidylinositol 4-kinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. phosphatidylinositol biosynthetic process Source: Reactome
  2. phospholipid metabolic process Source: Reactome
  3. small molecule metabolic process Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS00529-MONOMER.
ReactomeiREACT_120756. Synthesis of PIPs at the early endosome membrane.
REACT_120836. Synthesis of PIPs at the Golgi membrane.
REACT_121025. Synthesis of PIPs at the plasma membrane.
REACT_121168. Synthesis of PIPs at the ER membrane.
SignaLinkiQ8TCG2.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 4-kinase type 2-beta (EC:2.7.1.67)
Alternative name(s):
Phosphatidylinositol 4-kinase type II-beta
Short name:
PI4KII-BETA
Gene namesi
Name:PI4K2B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 4

Organism-specific databases

HGNCiHGNC:18215. PI4K2B.

Subcellular locationi

Cytoplasm. Membrane
Note: Mostly cytoplasmic but also found associated with the plasma membrane, the Golgi and endosomes. Compared to PI4K2A, a larger fraction of PI4K2B is cytosolic due to a smaller extent of palmitoylation. Translocates to membranes where it is recruited by PDGF stimulation by a Rac-GTP-dependent mechanism.

GO - Cellular componenti

  1. cytoplasm Source: HPA
  2. cytosol Source: Reactome
  3. membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142671173.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 481481Phosphatidylinositol 4-kinase type 2-betaPRO_0000285164Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei12 – 121Phosphoserine1 Publication
Modified residuei17 – 171Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8TCG2.
PaxDbiQ8TCG2.
PRIDEiQ8TCG2.

PTM databases

PhosphoSiteiQ8TCG2.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiQ8TCG2.
CleanExiHS_PI4K2B.
ExpressionAtlasiQ8TCG2. baseline and differential.
GenevestigatoriQ8TCG2.

Organism-specific databases

HPAiHPA004099.

Interactioni

Protein-protein interaction databases

BioGridi120587. 11 interactions.
IntActiQ8TCG2. 1 interaction.
STRINGi9606.ENSP00000264864.

Structurei

3D structure databases

ProteinModelPortaliQ8TCG2.
SMRiQ8TCG2. Positions 96-450.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini129 – 436308PI3K/PI4KAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi19 – 224Poly-Glu

Sequence similaritiesi

Contains 1 PI3K/PI4K domain.Curated

Phylogenomic databases

eggNOGiNOG287949.
GeneTreeiENSGT00390000010434.
HOGENOMiHOG000294076.
HOVERGENiHBG059542.
InParanoidiQ8TCG2.
KOiK13711.
OMAiPRIAWAH.
PhylomeDBiQ8TCG2.
TreeFamiTF314740.

Family and domain databases

InterProiIPR000403. PI3/4_kinase_cat_dom.
[Graphical view]
PfamiPF00454. PI3_PI4_kinase. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8TCG2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEDPSEPDRL ASADGGSPEE EEDGEREPLL PRIAWAHPRR GAPGSAVRLL
60 70 80 90 100
DAAGEEGEAG DEELPLPPGD VGVSRSSSAE LDRSRPAVSV TIGTSEMNAF
110 120 130 140 150
LDDPEFADIM LRAEQAIEVG IFPERISQGS SGSYFVKDPK RKIIGVFKPK
160 170 180 190 200
SEEPYGQLNP KWTKYVHKVC CPCCFGRGCL IPNQGYLSEA GAYLVDNKLH
210 220 230 240 250
LSIVPKTKVV WLVSETFNYN AIDRAKSRGK KYALEKVPKV GRKFHRIGLP
260 270 280 290 300
PKIGSFQLFV EGYKEAEYWL RKFEADPLPE NIRKQFQSQF ERLVILDYII
310 320 330 340 350
RNTDRGNDNW LVRYEKQKCE KEIDHKESKW IDDEEFLIKI AAIDNGLAFP
360 370 380 390 400
FKHPDEWRAY PFHWAWLPQA KVPFSEEIRN LILPYISDMN FVQDLCEDLY
410 420 430 440 450
ELFKTDKGFD KATFESQMSV MRGQILNLTQ ALRDGKSPFQ LVQIPCVIVE
460 470 480
RSQGGSQGRI VHLSNSFTQT VNCRKPFFSS W
Length:481
Mass (Da):54,744
Last modified:June 1, 2002 - v1
Checksum:iB4E16257E0AEC026
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti78 – 781S → P.5 Publications
Corresponds to variant rs313549 [ dbSNP | Ensembl ].
VAR_031974

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY065990 mRNA. Translation: AAL47580.1.
AF411320 mRNA. Translation: AAL04154.1.
AY091514 mRNA. Translation: AAM12049.1.
AK001967 mRNA. Translation: BAA92006.1.
BC051749 mRNA. Translation: AAH51749.1.
CCDSiCCDS3433.1.
RefSeqiNP_060793.2. NM_018323.3.
UniGeneiHs.191701.

Genome annotation databases

EnsembliENST00000264864; ENSP00000264864; ENSG00000038210.
GeneIDi55300.
KEGGihsa:55300.
UCSCiuc003grk.2. human.

Polymorphism databases

DMDMi74715788.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY065990 mRNA. Translation: AAL47580.1 .
AF411320 mRNA. Translation: AAL04154.1 .
AY091514 mRNA. Translation: AAM12049.1 .
AK001967 mRNA. Translation: BAA92006.1 .
BC051749 mRNA. Translation: AAH51749.1 .
CCDSi CCDS3433.1.
RefSeqi NP_060793.2. NM_018323.3.
UniGenei Hs.191701.

3D structure databases

ProteinModelPortali Q8TCG2.
SMRi Q8TCG2. Positions 96-450.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 120587. 11 interactions.
IntActi Q8TCG2. 1 interaction.
STRINGi 9606.ENSP00000264864.

Chemistry

ChEMBLi CHEMBL2096619.
GuidetoPHARMACOLOGYi 2499.

PTM databases

PhosphoSitei Q8TCG2.

Polymorphism databases

DMDMi 74715788.

Proteomic databases

MaxQBi Q8TCG2.
PaxDbi Q8TCG2.
PRIDEi Q8TCG2.

Protocols and materials databases

DNASUi 55300.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000264864 ; ENSP00000264864 ; ENSG00000038210 .
GeneIDi 55300.
KEGGi hsa:55300.
UCSCi uc003grk.2. human.

Organism-specific databases

CTDi 55300.
GeneCardsi GC04P025163.
HGNCi HGNC:18215. PI4K2B.
HPAi HPA004099.
MIMi 612101. gene.
neXtProti NX_Q8TCG2.
PharmGKBi PA142671173.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG287949.
GeneTreei ENSGT00390000010434.
HOGENOMi HOG000294076.
HOVERGENi HBG059542.
InParanoidi Q8TCG2.
KOi K13711.
OMAi PRIAWAH.
PhylomeDBi Q8TCG2.
TreeFami TF314740.

Enzyme and pathway databases

BioCyci MetaCyc:HS00529-MONOMER.
Reactomei REACT_120756. Synthesis of PIPs at the early endosome membrane.
REACT_120836. Synthesis of PIPs at the Golgi membrane.
REACT_121025. Synthesis of PIPs at the plasma membrane.
REACT_121168. Synthesis of PIPs at the ER membrane.
SignaLinki Q8TCG2.

Miscellaneous databases

ChiTaRSi PI4K2B. human.
GeneWikii PI4K2B.
GenomeRNAii 55300.
NextBioi 59520.
PROi Q8TCG2.
SOURCEi Search...

Gene expression databases

Bgeei Q8TCG2.
CleanExi HS_PI4K2B.
ExpressionAtlasi Q8TCG2. baseline and differential.
Genevestigatori Q8TCG2.

Family and domain databases

InterProi IPR000403. PI3/4_kinase_cat_dom.
[Graphical view ]
Pfami PF00454. PI3_PI4_kinase. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of type II phosphatidylinositol 4-kinase isoforms reveals association of the enzymes with endosomal vesicular compartments."
    Balla A., Tuymetova G., Barshishat M., Geiszt M., Balla T.
    J. Biol. Chem. 277:20041-20050(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, FUNCTION, SUBCELLULAR LOCATION, VARIANT PRO-78.
  2. "Type II phosphatidylinositol 4-kinase beta is a cytosolic and peripheral membrane protein that is recruited to the plasma membrane and activated by Rac-GTP."
    Wei Y.J., Sun H.Q., Yamamoto M., Wlodarski P., Kunii K., Martinez M., Barylko B., Albanesi J.P., Yin H.L.
    J. Biol. Chem. 277:46586-46593(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, ENZYME REGULATION, VARIANT PRO-78.
    Tissue: Brain.
  3. "Beta isoform of human type II phosphatidylinositol 4-kinase (PI4KIIbeta)."
    Hong W.
    Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PRO-78.
    Tissue: Placenta.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PRO-78.
    Tissue: Skin.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-12 AND SER-17, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANT [LARGE SCALE ANALYSIS] PRO-78, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.

Entry informationi

Entry nameiP4K2B_HUMAN
AccessioniPrimary (citable) accession number: Q8TCG2
Secondary accession number(s): Q9NUW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: June 1, 2002
Last modified: November 26, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3