Q8TCG2 (P4K2B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphatidylinositol 4-kinase type 2-beta
EC=2.7.1.67

Alternative name(s):
Phosphatidylinositol 4-kinase type II-beta
Short name=PI4KII-BETA

Gene names

Name:

PI4K2B

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	481 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Together with PI4K2A and the type III PI4Ks (PIK4CA and PIK4CB) it contributes to the overall PI4-kinase activity of the cell. This contribution may be especially significant in plasma membrane, endosomal and Golgi compartments. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). Contributes to the production of InsP3 in stimulated cells and is likely to be involved in the regulation of vesicular trafficking. Ref.1
Catalytic activity	ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate.
Enzyme regulation	Recruited and activated by membrane association. Binding to the microsomal membrane has been shown to enhance its activity. Ref.2
Subcellular location	Cytoplasm. Membrane. Note: Mostly cytoplasmic but also found associated with the plasma membrane, the Golgi and endosomes. Compared to PI4K2A, a larger fraction of PI4K2B is cytosolic due to a smaller extent of palmitoylation. Translocates to membranes where it is recruited by PDGF stimulation by a Rac-GTP-dependent mechanism. Ref.1 Ref.2
Tissue specificity	Widely expressed. Ref.1
Sequence similarities	Belongs to the PI3/PI4-kinase family. Type II PI4K subfamily. Contains 1 PI3K/PI4K domain.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane
Coding sequence diversity	Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from direct assay. Source: HPA membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	1-phosphatidylinositol 4-kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 481	481	Phosphatidylinositol 4-kinase type 2-beta		PRO_0000285164
Regions
	Domain	129 – 436	308	PI3K/PI4K
	Compositional bias	19 – 22	4	Poly-Glu
Amino acid modifications
	Modified residue	12	1	Phosphoserine Ref.6
	Modified residue	17	1	Phosphoserine Ref.6
	Modified residue	45	1	Phosphoserine Ref.6
Natural variations
	Natural variant	78	1	S → P. Ref.1 Ref.2 Ref.4 Ref.5 Corresponds to variant rs313549 [ dbSNP | Ensembl ].		VAR_031974

Sequences

	Sequence		Length	Mass (Da)
	Q8TCG2 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: B4E16257E0AEC026	FASTA	481	54,744
	10 20 30 40 50 60 MEDPSEPDRL ASADGGSPEE EEDGEREPLL PRIAWAHPRR GAPGSAVRLL DAAGEEGEAG 70 80 90 100 110 120 DEELPLPPGD VGVSRSSSAE LDRSRPAVSV TIGTSEMNAF LDDPEFADIM LRAEQAIEVG 130 140 150 160 170 180 IFPERISQGS SGSYFVKDPK RKIIGVFKPK SEEPYGQLNP KWTKYVHKVC CPCCFGRGCL 190 200 210 220 230 240 IPNQGYLSEA GAYLVDNKLH LSIVPKTKVV WLVSETFNYN AIDRAKSRGK KYALEKVPKV 250 260 270 280 290 300 GRKFHRIGLP PKIGSFQLFV EGYKEAEYWL RKFEADPLPE NIRKQFQSQF ERLVILDYII 310 320 330 340 350 360 RNTDRGNDNW LVRYEKQKCE KEIDHKESKW IDDEEFLIKI AAIDNGLAFP FKHPDEWRAY 370 380 390 400 410 420 PFHWAWLPQA KVPFSEEIRN LILPYISDMN FVQDLCEDLY ELFKTDKGFD KATFESQMSV 430 440 450 460 470 480 MRGQILNLTQ ALRDGKSPFQ LVQIPCVIVE RSQGGSQGRI VHLSNSFTQT VNCRKPFFSS W

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of type II phosphatidylinositol 4-kinase isoforms reveals association of the enzymes with endosomal vesicular compartments." Balla A., Tuymetova G., Barshishat M., Geiszt M., Balla T. J. Biol. Chem. 277:20041-20050(2002) [PubMed: 11923287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, FUNCTION, SUBCELLULAR LOCATION, VARIANT PRO-78.
[2]	"Type II phosphatidylinositol 4-kinase beta is a cytosolic and peripheral membrane protein that is recruited to the plasma membrane and activated by Rac-GTP." Wei Y.J., Sun H.Q., Yamamoto M., Wlodarski P., Kunii K., Martinez M., Barylko B., Albanesi J.P., Yin H.L. J. Biol. Chem. 277:46586-46593(2002) [PubMed: 12324459] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, ENZYME REGULATION, VARIANT PRO-78. Tissue: Brain.
[3]	"Beta isoform of human type II phosphatidylinositol 4-kinase (PI4KIIbeta)." Hong W. Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PRO-78. Tissue: Placenta.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PRO-78. Tissue: Skin.
[6]	"Large-scale proteomics analysis of the human kinome." Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H. Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed: 19369195] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-12; SER-17 AND SER-45, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY065990 mRNA. Translation: AAL47580.1. AF411320 mRNA. Translation: AAL04154.1. AY091514 mRNA. Translation: AAM12049.1. AK001967 mRNA. Translation: BAA92006.1. BC051749 mRNA. Translation: AAH51749.1.
IPI	IPI00291068.
RefSeq	NP_060793.2. NM_018323.3.
UniGene	Hs.726376.
3D structure databases
ProteinModelPortal	Q8TCG2.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8TCG2. 1 interaction.
STRING	Q8TCG2.
PTM databases
PhosphoSite	Q8TCG2.
Polymorphism databases
DMDM	74715788.
Proteomic databases
PRIDE	Q8TCG2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000264864; ENSP00000264864; ENSG00000038210.
GeneID	55300.
KEGG	hsa:55300.
UCSC	uc003grk.1. human.
Organism-specific databases
CTD	55300.
GeneCards	GC04P025236.
H-InvDB	HIX0020484.
HGNC	HGNC:18215. PI4K2B.
HPA	HPA004099.
MIM	612101. gene.
neXtProt	NX_Q8TCG2.
PharmGKB	PA142671173.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG13238.
GeneTree	ENSGT00390000010434.
HOGENOM	HBG714361.
HOVERGEN	HBG059542.
InParanoid	Q8TCG2.
OMA	AEQAIEF.
PhylomeDB	Q8TCG2.
Enzyme and pathway databases
BioCyc	MetaCyc:ENSG00000038210-MONOMER.
Gene expression databases
ArrayExpress	Q8TCG2.
Bgee	Q8TCG2.
CleanEx	HS_PI4K2B.
Genevestigator	Q8TCG2.
Family and domain databases
InterPro	IPR000403. PI3/4_kinase_cat. [Graphical view]
KO	K13711.
Pfam	PF00454. PI3_PI4_kinase. 1 hit. [Graphical view]
PROSITE	PS00915. PI3_4_KINASE_1. False negative. PS00916. PI3_4_KINASE_2. False negative. PS50290. PI3_4_KINASE_3. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	59520.
SOURCE	Search...

Entry information

Entry name

P4K2B_HUMAN

Accession

Primary (citable) accession number: Q8TCG2
Secondary accession number(s): Q9NUW2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	June 1, 2002
Last modified:	January 25, 2012
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.