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Protein

Pyridoxal phosphate phosphatase PHOSPHO2

Gene

PHOSPHO2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Phosphatase that has high activity toward pyridoxal 5'-phosphate (PLP). Also active at much lower level toward pyrophosphate, phosphoethanolamine (PEA), phosphocholine (PCho), phospho-l-tyrosine, fructose-6-phosphate, p-nitrophenyl phosphate, and h-glycerophosphate.

Catalytic activityi

Pyridoxal 5'-phosphate + H2O = pyridoxal + phosphate.1 Publication

Cofactori

Mg2+By similarity

Kineticsi

  1. KM=45.5 µM for pyridoxal 5'-phosphate1 Publication
  1. Vmax=633 nmol/min/mg enzyme with pyridoxal 5'-phosphate as substrate1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei8 – 81NucleophileBy similarity
Metal bindingi8 – 81MagnesiumBy similarity
Active sitei10 – 101Proton donorBy similarity
Metal bindingi10 – 101MagnesiumBy similarity
Binding sitei19 – 191SubstrateBy similarity
Binding sitei99 – 991SubstrateBy similarity
Metal bindingi179 – 1791MagnesiumBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Pyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:HS07168-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyridoxal phosphate phosphatase PHOSPHO2 (EC:3.1.3.74)
Gene namesi
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:28316. PHOSPHO2.

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134947617.

Polymorphism and mutation databases

BioMutaiPHOSPHO2.
DMDMi74730590.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 241241Pyridoxal phosphate phosphatase PHOSPHO2PRO_0000068833Add
BLAST

Proteomic databases

PaxDbiQ8TCD6.
PRIDEiQ8TCD6.

PTM databases

DEPODiQ8TCD6.

Expressioni

Gene expression databases

BgeeiQ8TCD6.
ExpressionAtlasiQ8TCD6. baseline and differential.
GenevisibleiQ8TCD6. HS.

Organism-specific databases

HPAiHPA034726.
HPA034727.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
NAB2Q157423EBI-2861380,EBI-8641936

Protein-protein interaction databases

BioGridi138930. 4 interactions.
IntActiQ8TCD6. 2 interactions.
STRINGi9606.ENSP00000352782.

Structurei

3D structure databases

ProteinModelPortaliQ8TCD6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3120. Eukaryota.
ENOG4111NAQ. LUCA.
GeneTreeiENSGT00390000007741.
HOGENOMiHOG000231038.
HOVERGENiHBG080058.
InParanoidiQ8TCD6.
KOiK13248.
OMAiGNDFCPV.
OrthoDBiEOG7BZVTZ.
PhylomeDBiQ8TCD6.
TreeFamiTF300112.

Family and domain databases

Gene3Di3.40.50.1000. 2 hits.
InterProiIPR023214. HAD-like_dom.
IPR006383. HAD-SF_hydro_IB_PSP-like.
IPR016965. Pase_PHOSPHO-typ.
IPR006384. PyrdxlP_Pase-rel.
[Graphical view]
PfamiPF06888. Put_Phosphatase. 1 hit.
[Graphical view]
PIRSFiPIRSF031051. PyrdxlP_Pase_PHOSPHO2. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01489. DKMTPPase-SF. 1 hit.
TIGR01488. HAD-SF-IB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8TCD6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKILLVFDFD NTIIDDNSDT WIVQCAPNKK LPIELRDSYR KGFWTEFMGR
60 70 80 90 100
VFKYLGDKGV REHEMKRAVT SLPFTPGMVE LFNFIRKNKD KFDCIIISDS
110 120 130 140 150
NSVFIDWVLE AASFHDIFDK VFTNPAAFNS NGHLTVENYH THSCNRCPKN
160 170 180 190 200
LCKKVVLIEF VDKQLQQGVN YTQIVYIGDG GNDVCPVTFL KNDDVAMPRK
210 220 230 240
GYTLQKTLSR MSQNLEPMEY SVVVWSSGVD IISHLQFLIK D
Length:241
Mass (Da):27,769
Last modified:June 1, 2002 - v1
Checksum:iC9F0447F2A1A5FFA
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti206 – 2061K → E.
Corresponds to variant rs56036676 [ dbSNP | Ensembl ].
VAR_062092

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK314915 mRNA. Translation: BAG37427.1.
AC016772 Genomic DNA. Translation: AAY24234.1.
CH471058 Genomic DNA. Translation: EAX11262.1.
CH471058 Genomic DNA. Translation: EAX11263.1.
BC022324 mRNA. Translation: AAH22324.1.
BC106013 mRNA. Translation: AAI06014.1.
CCDSiCCDS33319.1.
RefSeqiNP_001008489.1. NM_001008489.3.
NP_001186214.1. NM_001199285.1.
NP_001186215.1. NM_001199286.1.
NP_001186216.1. NM_001199287.1.
NP_001186217.1. NM_001199288.1.
UniGeneiHs.745233.

Genome annotation databases

EnsembliENST00000359744; ENSP00000352782; ENSG00000144362.
ENST00000616481; ENSP00000481680; ENSG00000144362.
ENST00000616524; ENSP00000481046; ENSG00000144362.
ENST00000617738; ENSP00000481857; ENSG00000144362.
GeneIDi493911.
KEGGihsa:493911.
UCSCiuc002ufg.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK314915 mRNA. Translation: BAG37427.1.
AC016772 Genomic DNA. Translation: AAY24234.1.
CH471058 Genomic DNA. Translation: EAX11262.1.
CH471058 Genomic DNA. Translation: EAX11263.1.
BC022324 mRNA. Translation: AAH22324.1.
BC106013 mRNA. Translation: AAI06014.1.
CCDSiCCDS33319.1.
RefSeqiNP_001008489.1. NM_001008489.3.
NP_001186214.1. NM_001199285.1.
NP_001186215.1. NM_001199286.1.
NP_001186216.1. NM_001199287.1.
NP_001186217.1. NM_001199288.1.
UniGeneiHs.745233.

3D structure databases

ProteinModelPortaliQ8TCD6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi138930. 4 interactions.
IntActiQ8TCD6. 2 interactions.
STRINGi9606.ENSP00000352782.

PTM databases

DEPODiQ8TCD6.

Polymorphism and mutation databases

BioMutaiPHOSPHO2.
DMDMi74730590.

Proteomic databases

PaxDbiQ8TCD6.
PRIDEiQ8TCD6.

Protocols and materials databases

DNASUi493911.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000359744; ENSP00000352782; ENSG00000144362.
ENST00000616481; ENSP00000481680; ENSG00000144362.
ENST00000616524; ENSP00000481046; ENSG00000144362.
ENST00000617738; ENSP00000481857; ENSG00000144362.
GeneIDi493911.
KEGGihsa:493911.
UCSCiuc002ufg.4. human.

Organism-specific databases

CTDi493911.
GeneCardsiPHOSPHO2.
HGNCiHGNC:28316. PHOSPHO2.
HPAiHPA034726.
HPA034727.
neXtProtiNX_Q8TCD6.
PharmGKBiPA134947617.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3120. Eukaryota.
ENOG4111NAQ. LUCA.
GeneTreeiENSGT00390000007741.
HOGENOMiHOG000231038.
HOVERGENiHBG080058.
InParanoidiQ8TCD6.
KOiK13248.
OMAiGNDFCPV.
OrthoDBiEOG7BZVTZ.
PhylomeDBiQ8TCD6.
TreeFamiTF300112.

Enzyme and pathway databases

BioCyciMetaCyc:HS07168-MONOMER.

Miscellaneous databases

GenomeRNAii493911.
NextBioi111796.
PROiQ8TCD6.

Gene expression databases

BgeeiQ8TCD6.
ExpressionAtlasiQ8TCD6. baseline and differential.
GenevisibleiQ8TCD6. HS.

Family and domain databases

Gene3Di3.40.50.1000. 2 hits.
InterProiIPR023214. HAD-like_dom.
IPR006383. HAD-SF_hydro_IB_PSP-like.
IPR016965. Pase_PHOSPHO-typ.
IPR006384. PyrdxlP_Pase-rel.
[Graphical view]
PfamiPF06888. Put_Phosphatase. 1 hit.
[Graphical view]
PIRSFiPIRSF031051. PyrdxlP_Pase_PHOSPHO2. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01489. DKMTPPase-SF. 1 hit.
TIGR01488. HAD-SF-IB. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  2. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Bone and Lung.
  5. Cited for: ENZYME ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiPHOP2_HUMAN
AccessioniPrimary (citable) accession number: Q8TCD6
Secondary accession number(s): B2RC30, D3DPC7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: June 1, 2002
Last modified: March 16, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.