Q8TCD6 (PHOP2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyridoxal phosphate phosphatase PHOSPHO2
EC=3.1.3.74

Gene names

Name:

PHOSPHO2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	241 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Phosphatase that has high activity toward pyridoxal 5'-phosphate (PLP). Also active at much lower level toward pyrophosphate, phosphoethanolamine (PEA), phosphocholine (PCho), phospho-l-tyrosine, fructose-6-phosphate, p-nitrophenyl phosphate, and h-glycerophosphate.
Catalytic activity	Pyridoxal 5'-phosphate + H2O = pyridoxal + phosphate. Ref.5
Cofactor	Magnesium By similarity.
Sequence similarities	Belongs to the HAD-like hydrolase superfamily. PHOSPHO family.
Biophysicochemical properties	Kinetic parameters: KM=45.5 µM for pyridoxal 5'-phosphate Ref.5 Vmax=633 nmol/min/mg enzyme with pyridoxal 5'-phosphate as substrate

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Ligand	Magnesium Metal-binding Pyridoxal phosphate
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW pyridoxal phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 241	241	Pyridoxal phosphate phosphatase PHOSPHO2		PRO_0000068833
Sites
	Active site	8	1	Nucleophile By similarity
	Active site	10	1	Proton donor By similarity
	Metal binding	8	1	Magnesium By similarity
	Metal binding	10	1	Magnesium By similarity
	Metal binding	179	1	Magnesium By similarity
	Binding site	19	1	Substrate By similarity
	Binding site	99	1	Substrate By similarity
Natural variations
	Natural variant	206	1	K → E. Corresponds to variant rs56036676 [ dbSNP | Ensembl ].		VAR_062092

Sequences

	Sequence		Length	Mass (Da)
	Q8TCD6 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: C9F0447F2A1A5FFA	FASTA	241	27,769
	10 20 30 40 50 60 MKILLVFDFD NTIIDDNSDT WIVQCAPNKK LPIELRDSYR KGFWTEFMGR VFKYLGDKGV 70 80 90 100 110 120 REHEMKRAVT SLPFTPGMVE LFNFIRKNKD KFDCIIISDS NSVFIDWVLE AASFHDIFDK 130 140 150 160 170 180 VFTNPAAFNS NGHLTVENYH THSCNRCPKN LCKKVVLIEF VDKQLQQGVN YTQIVYIGDG 190 200 210 220 230 240 GNDVCPVTFL KNDDVAMPRK GYTLQKTLSR MSQNLEPMEY SVVVWSSGVD IISHLQFLIK D

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[2]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Bone and Lung.
[5]	"Probing the substrate specificities of human PHOSPHO1 and PHOSPHO2." Roberts S.J., Stewart A.J., Schmid R., Blindauer C.A., Bond S.R., Sadler P.J., Farquharson C. Biochim. Biophys. Acta 1752:73-82(2005) [PubMed: 16054448] [Abstract] Cited for: ENZYME ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK314915 mRNA. Translation: BAG37427.1. AC016772 Genomic DNA. Translation: AAY24234.1. CH471058 Genomic DNA. Translation: EAX11262.1. CH471058 Genomic DNA. Translation: EAX11263.1. BC022324 mRNA. Translation: AAH22324.1. BC106013 mRNA. Translation: AAI06014.1.
IPI	IPI00152344.
RefSeq	NP_001008489.1. NM_001008489.3. NP_001186214.1. NM_001199285.1. NP_001186215.1. NM_001199286.1. NP_001186216.1. NM_001199287.1. NP_001186217.1. NM_001199288.1.
UniGene	Hs.655150.
3D structure databases
ProteinModelPortal	Q8TCD6.
SMR	Q8TCD6. Positions 144-199.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8TCD6. 1 interaction.
STRING	Q8TCD6.
Polymorphism databases
DMDM	74730590.
Proteomic databases
PRIDE	Q8TCD6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000359744; ENSP00000352782; ENSG00000144362.
GeneID	493911.
KEGG	hsa:493911.
UCSC	uc002ufg.1. human.
Organism-specific databases
CTD	493911.
GeneCards	GC02P170550.
HGNC	HGNC:28316. PHOSPHO2.
HPA	HPA034726.
neXtProt	NX_Q8TCD6.
PharmGKB	PA134947617.
GenAtlas	Search...
Phylogenomic databases
GeneTree	ENSGT00390000007741.
HOGENOM	HBG319786.
HOVERGEN	HBG080058.
InParanoid	Q8TCD6.
OMA	DWVLEAT.
OrthoDB	EOG4S4PH0.
PhylomeDB	Q8TCD6.
Gene expression databases
ArrayExpress	Q8TCD6.
Bgee	Q8TCD6.
Genevestigator	Q8TCD6.
GermOnline	ENSG00000144362. Homo sapiens.
Family and domain databases
InterPro	IPR023214. HAD-like_dom. IPR006383. HAD-SF_hydro_IB_PSP-like. IPR016965. Pase_PHOSPHO-typ. IPR006384. PyrdxlP_Pase-rel. [Graphical view]
Gene3D	G3DSA:3.40.50.1000. HAD-like_dom. 2 hits.
KO	K13248.
Pfam	PF06888. Put_Phosphatase. 1 hit. [Graphical view]
PIRSF	PIRSF031051. PyrdxlP_Pase_PHOSPHO2. 1 hit.
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01489. DKMTPPase-SF. 1 hit. TIGR01488. HAD-SF-IB. 1 hit.
ProtoNet	Search...
Other
NextBio	111796.

Entry information

Entry name

PHOP2_HUMAN

Accession

Primary (citable) accession number: Q8TCD6
Secondary accession number(s): B2RC30, D3DPC7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	June 1, 2002
Last modified:	January 25, 2012
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.