Reviewed, UniProtKB/Swiss-Prot Q8TCD6 (PHOP2_HUMAN)

Names and origin

Protein names

Recommended name:
    Pyridoxal phosphate phosphatase PHOSPHO2
    EC=3.1.3.74

Gene names

Name:

PHOSPHO2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	241 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Phosphatase that has high activity toward pyridoxal 5'-phosphate (PLP). Also active at much lower level toward pyrophosphate, phosphoethanolamine (PEA), phosphocholine (PCho), phospho-l-tyrosine, fructose-6-phosphate, p-nitrophenyl phosphate, and h-glycerophosphate.
Catalytic activity	Pyridoxal 5'-phosphate + H2O = pyridoxal + phosphate. Ref.3
Cofactor	Magnesium By similarity.
Sequence similarities	Belongs to the HAD-like hydrolase superfamily. PHOSPHO family.
biophysicochemical properties	Kinetic parameters: KM=45.5 µM for pyridoxal 5'-phosphate Vmax=633 nmol/min/mg enzyme with pyridoxal 5'-phosphate as substrate

Ontologies

Keywords
Ligand	Magnesium Metal-binding Pyridoxal phosphate
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW pyridoxal phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 241	241	Pyridoxal phosphate phosphatase PHOSPHO2		PRO_0000068833
Sites
	Active site	8	1	Nucleophile By similarity
	Active site	10	1	Proton donor By similarity
	Metal binding	8	1	Magnesium By similarity
	Metal binding	10	1	Magnesium By similarity
	Metal binding	179	1	Magnesium By similarity
	Binding site	19	1	Substrate By similarity
	Binding site	99	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8TCD6-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: C9F0447F2A1A5FFA	FASTA	241	27,769
	10 20 30 40 50 60 MKILLVFDFD NTIIDDNSDT WIVQCAPNKK LPIELRDSYR KGFWTEFMGR VFKYLGDKGV 70 80 90 100 110 120 REHEMKRAVT SLPFTPGMVE LFNFIRKNKD KFDCIIISDS NSVFIDWVLE AASFHDIFDK 130 140 150 160 170 180 VFTNPAAFNS NGHLTVENYH THSCNRCPKN LCKKVVLIEF VDKQLQQGVN YTQIVYIGDG 190 200 210 220 230 240 GNDVCPVTFL KNDDVAMPRK GYTLQKTLSR MSQNLEPMEY SVVVWSSGVD IISHLQFLIK D

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Bone and Lung.
[3]	"Probing the substrate specificities of human PHOSPHO1 and PHOSPHO2." Roberts S.J., Stewart A.J., Schmid R., Blindauer C.A., Bond S.R., Sadler P.J., Farquharson C. Biochim. Biophys. Acta 1752:73-82(2005) [PubMed: 16054448] [Abstract] Cited for: ENZYME ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC016772 Genomic DNA. Translation: AAY24234.1. BC022324 mRNA. Translation: AAH22324.1. BC106013 mRNA. Translation: AAI06014.1.
IPI	IPI00152344.
RefSeq	NP_001008489.1.
UniGene	Hs.655150
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q8TCD6.
Genome annotation databases
Ensembl	ENSG00000144362. Homo sapiens. [Contig view]
GeneID	493911.
KEGG	hsa:493911.
Organism-specific databases
GeneCards	GC02P170259.
H-InvDB	HIX0002574.
HGNC	HGNC:28316. PHOSPHO2.
PharmGKB	PA134947617.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q8TCD6.
OMA	Q8TCD6. NDVAMPR.
Enzyme and pathway databases
BRENDA	3.1.3.74. 247.
Gene expression databases
ArrayExpress	Q8TCD6.
Bgee	Q8TCD6.
GermOnline	ENSG00000144362. Homo sapiens.
Family and domain databases
InterPro	IPR006383. HAD-SF_hydro_IB_PSP-like. IPR006384. PyrdxlP_Pase-rel. IPR016965. PyrdxlP_Pase_PHOSPHO2. [Graphical view]
Pfam	PF06888. Put_Phosphatase. 1 hit. [Graphical view]
PIRSF	PIRSF031051. PyrdxlP_Pase_PHOSPHO2. 1 hit.
TIGRFAMs	TIGR01489. DKMTPPase-SF. 1 hit. TIGR01488. HAD-SF-IB. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	111796.

Entry information

Entry name

PHOP2_HUMAN

Accession

Primary (citable) accession number: Q8TCD6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	June 1, 2002
Last modified:	June 16, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)