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Protein

Solute carrier family 22 member 8

Gene

SLC22A8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an important role in the excretion/detoxification of endogenous and exogenous organic anions, especially from the brain and kidney. Involved in the transport basolateral of steviol, fexofenadine. Transports benzylpenicillin (PCG), estrone-3-sulfate (E1S), cimetidine (CMD), 2,4-dichloro-phenoxyacetate (2,4-D), p-amino-hippurate (PAH), acyclovir (ACV) and ochratoxin (OTA).4 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processDetoxification, Ion transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-561048 Organic anion transport

Protein family/group databases

TCDBi2.A.1.19.34 the major facilitator superfamily (mfs)

Names & Taxonomyi

Protein namesi
Recommended name:
Solute carrier family 22 member 8
Alternative name(s):
Organic anion transporter 3
Short name:
hOAT3
Gene namesi
Name:SLC22A8
Synonyms:OAT3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000149452.15
HGNCiHGNC:10972 SLC22A8
MIMi607581 gene
neXtProtiNX_Q8TCC7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 9CytoplasmicSequence analysis9
Transmembranei10 – 30HelicalSequence analysisAdd BLAST21
Topological domaini31 – 123ExtracellularSequence analysisAdd BLAST93
Transmembranei124 – 144HelicalSequence analysisAdd BLAST21
Topological domaini145 – 154CytoplasmicSequence analysis10
Transmembranei155 – 175HelicalSequence analysisAdd BLAST21
Topological domaini176ExtracellularSequence analysis1
Transmembranei177 – 197HelicalSequence analysisAdd BLAST21
Topological domaini198 – 212CytoplasmicSequence analysisAdd BLAST15
Transmembranei213 – 233HelicalSequence analysisAdd BLAST21
Topological domaini234 – 236ExtracellularSequence analysis3
Transmembranei237 – 257HelicalSequence analysisAdd BLAST21
Topological domaini258 – 327CytoplasmicSequence analysisAdd BLAST70
Transmembranei328 – 348HelicalSequence analysisAdd BLAST21
Topological domaini349 – 354ExtracellularSequence analysis6
Transmembranei355 – 375HelicalSequence analysisAdd BLAST21
Topological domaini376 – 386CytoplasmicSequence analysisAdd BLAST11
Transmembranei387 – 407HelicalSequence analysisAdd BLAST21
Topological domaini408 – 411ExtracellularSequence analysis4
Transmembranei412 – 432HelicalSequence analysisAdd BLAST21
Topological domaini433 – 471CytoplasmicSequence analysisAdd BLAST39
Transmembranei472 – 492HelicalSequence analysisAdd BLAST21
Topological domaini493 – 542ExtracellularSequence analysisAdd BLAST50

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi9376
OpenTargetsiENSG00000149452
PharmGKBiPA389

Chemistry databases

ChEMBLiCHEMBL1641348
DrugBankiDB00787 Aciclovir
DB00718 Adefovir Dipivoxil
DB00437 Allopurinol
DB00345 Aminohippuric acid
DB00168 Aspartame
DB09060 Avibactam
DB00181 Baclofen
DB03793 Benzoic Acid
DB01053 Benzylpenicillin
DB00887 Bumetanide
DB04519 Caprylic acid
DB01414 Cefacetrile
DB01140 Cefadroxil
DB00456 Cefalotin
DB01326 Cefamandole
DB01327 Cefazolin
DB01329 Cefoperazone
DB00493 Cefotaxime
DB01212 Ceftriaxone
DB00567 Cephalexin
DB02659 Cholic Acid
DB01597 Cilastatin
DB00501 Cimetidine
DB00286 Conjugated Equine Estrogens
DB02527 Cyclic Adenosine Monophosphate
DB08912 Dabrafenib
DB04133 Degraded Cephaloridine
DB05804 dehydroepiandrosterone sulfate
DB00586 Diclofenac
DB00390 Digoxin
DB01160 Dinoprost Tromethamine
DB00917 Dinoprostone
DB00254 Doxycycline
DB09272 Eluxadoline
DB00584 Enalapril
DB00783 Estradiol
DB00927 Famotidine
DB00695 Furosemide
DB01004 Ganciclovir
DB03553 Glutaric Acid
DB00536 Guanidine
DB01050 Ibuprofen
DB00328 Indomethacin
DB01009 Ketoprofen
DB00583 L-Carnitine
DB00279 Liothyronine
DB01583 Liotrix
DB01065 Melatonin
DB01033 Mercaptopurine
DB00563 Methotrexate
DB06710 Methyltestosterone
DB01017 Minocycline
DB01051 Novobiocin
DB00198 Oseltamivir
DB01092 Ouabain
DB03902 Oxalic Acid
DB00595 Oxytetracycline
DB00812 Phenylbutazone
DB00554 Piroxicam
DB00175 Pravastatin
DB01032 Probenecid
DB00908 Quinidine
DB00863 Ranitidine
DB00936 Salicylic acid
DB06335 Saxagliptin
DB00139 Succinic acid
DB04348 Taurocholic Acid
DB00300 Tenofovir
DB00469 Tenoxicam
DB00624 Testosterone
DB00759 Tetracycline
DB08837 Tetraethylammonium
DB01650 trans-2-hydroxycinnamic acid
DB08844 Uric Acid
DB00577 Valaciclovir
DB00313 Valproic Acid
DB00495 Zidovudine
GuidetoPHARMACOLOGYi1027

Polymorphism and mutation databases

BioMutaiSLC22A8
DMDMi74730587

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002734391 – 542Solute carrier family 22 member 8Add BLAST542

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei4PhosphoserineBy similarity1
Glycosylationi86N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi102N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ8TCC7
PeptideAtlasiQ8TCC7
PRIDEiQ8TCC7

PTM databases

iPTMnetiQ8TCC7
PhosphoSitePlusiQ8TCC7
SwissPalmiQ8TCC7

Expressioni

Tissue specificityi

Expressed in kidney.1 Publication

Gene expression databases

BgeeiENSG00000149452
CleanExiHS_SLC22A8
ExpressionAtlasiQ8TCC7 baseline and differential
GenevisibleiQ8TCC7 HS

Organism-specific databases

HPAiHPA044174

Interactioni

Protein-protein interaction databases

BioGridi114777, 1 interactor
IntActiQ8TCC7, 1 interactor
STRINGi9606.ENSP00000337335

Chemistry databases

BindingDBiQ8TCC7

Structurei

3D structure databases

ProteinModelPortaliQ8TCC7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0255 Eukaryota
COG0477 LUCA
GeneTreeiENSGT00760000118852
HOGENOMiHOG000234569
HOVERGENiHBG108433
InParanoidiQ8TCC7
KOiK08205
OMAiMANHNLL
OrthoDBiEOG091G0738
PhylomeDBiQ8TCC7
TreeFamiTF315847

Family and domain databases

CDDicd06174 MFS, 1 hit
InterProiView protein in InterPro
IPR020846 MFS_dom
IPR005828 MFS_sugar_transport-like
IPR036259 MFS_trans_sf
IPR004749 Orgcat_transp/SVOP
IPR005829 Sugar_transporter_CS
PfamiView protein in Pfam
PF00083 Sugar_tr, 1 hit
SUPFAMiSSF103473 SSF103473, 1 hit
TIGRFAMsiTIGR00898 2A0119, 1 hit
PROSITEiView protein in PROSITE
PS50850 MFS, 1 hit

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8TCC7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTFSEILDRV GSMGHFQFLH VAILGLPILN MANHNLLQIF TAATPVHHCR
60 70 80 90 100
PPHNASTGPW VLPMGPNGKP ERCLRFVHPP NASLPNDTQR AMEPCLDGWV
110 120 130 140 150
YNSTKDSIVT EWDLVCNSNK LKEMAQSIFM AGILIGGLVL GDLSDRFGRR
160 170 180 190 200
PILTCSYLLL AASGSGAAFS PTFPIYMVFR FLCGFGISGI TLSTVILNVE
210 220 230 240 250
WVPTRMRAIM STALGYCYTF GQFILPGLAY AIPQWRWLQL TVSIPFFVFF
260 270 280 290 300
LSSWWTPESI RWLVLSGKSS KALKILRRVA VFNGKKEEGE RLSLEELKLN
310 320 330 340 350
LQKEISLAKA KYTASDLFRI PMLRRMTFCL SLAWFATGFA YYSLAMGVEE
360 370 380 390 400
FGVNLYILQI IFGGVDVPAK FITILSLSYL GRHTTQAAAL LLAGGAILAL
410 420 430 440 450
TFVPLDLQTV RTVLAVFGKG CLSSSFSCLF LYTSELYPTV IRQTGMGVSN
460 470 480 490 500
LWTRVGSMVS PLVKITGEVQ PFIPNIIYGI TALLGGSAAL FLPETLNQPL
510 520 530 540
PETIEDLENW SLRAKKPKQE PEVEKASQRI PLQPHGPGLG SS
Length:542
Mass (Da):59,856
Last modified:June 1, 2002 - v1
Checksum:i22799360D91B4CCF
GO
Isoform 2 (identifier: Q8TCC7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     510-510: W → WSVTASGPPR

Note: No experimental confirmation available.
Show »
Length:551
Mass (Da):60,709
Checksum:i0F202C686929638A
GO
Isoform 5 (identifier: Q8TCC7-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-123: Missing.

Note: No experimental confirmation available.
Show »
Length:419
Mass (Da):46,120
Checksum:i3BBFC757CEE06790
GO
Isoform 3 (identifier: Q8TCC7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-345: Missing.
     406-455: DLQTVRTVLA...MGVSNLWTRV → GERLGLPQNP...SGAQGLSSRK
     456-542: Missing.

Note: No experimental confirmation available.
Show »
Length:110
Mass (Da):11,502
Checksum:i0050E6499A6446CB
GO
Isoform 4 (identifier: Q8TCC7-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-91: Missing.

Note: No experimental confirmation available.
Show »
Length:451
Mass (Da):49,821
Checksum:iB1E5B7631D8BED48
GO

Sequence cautioni

The sequence AAD19357 differs from that shown. Reason: Frameshift at several positions.Curated
The sequence BAD92929 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti150Missing in AAD19357 (PubMed:10049739).Curated1
Sequence conflicti151P → T in AAD19357 (PubMed:10049739).Curated1
Sequence conflicti271K → E in BAB47393 (Ref. 2) Curated1
Sequence conflicti286Missing in AAD19357 (PubMed:10049739).Curated1
Sequence conflicti411R → E in AAD19357 (PubMed:10049739).Curated1
Sequence conflicti478 – 480YGI → FTGS in AAD19357 (PubMed:10049739).Curated3
Sequence conflicti526A → T in BAG60589 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_030146129F → L. Corresponds to variant dbSNP:rs11568479Ensembl.1
Natural variantiVAR_030147149R → S Complete loss of function. 1 PublicationCorresponds to variant dbSNP:rs45566039Ensembl.1
Natural variantiVAR_030148260I → R Complete loss of function. 1 PublicationCorresponds to variant dbSNP:rs11568493Ensembl.1
Natural variantiVAR_030149277R → W Reduced function. 1 PublicationCorresponds to variant dbSNP:rs11568492Ensembl.1
Natural variantiVAR_030150281V → A in 6% of African-Americans. Corresponds to variant dbSNP:rs45438191Ensembl.1
Natural variantiVAR_030151305I → F in 3.5% of Asian-American; reduced function. 1 PublicationCorresponds to variant dbSNP:rs11568482Ensembl.1
Natural variantiVAR_030152389A → V1 PublicationCorresponds to variant dbSNP:rs749944923Ensembl.1
Natural variantiVAR_030153448V → I. Corresponds to variant dbSNP:rs11568486Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0225621 – 345Missing in isoform 3. 1 PublicationAdd BLAST345
Alternative sequenceiVSP_0458241 – 123Missing in isoform 5. CuratedAdd BLAST123
Alternative sequenceiVSP_0452721 – 91Missing in isoform 4. 1 PublicationAdd BLAST91
Alternative sequenceiVSP_022563406 – 455DLQTV…LWTRV → GERLGLPQNPLEEAARLGAR DFTAGSASKSLCYLEQVPAL SGAQGLSSRK in isoform 3. 1 PublicationAdd BLAST50
Alternative sequenceiVSP_022564456 – 542Missing in isoform 3. 1 PublicationAdd BLAST87
Alternative sequenceiVSP_022565510W → WSVTASGPPR in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF097491 mRNA Translation: AAD19357.1 Frameshift.
AB042505 mRNA Translation: BAB47393.1
AK298341 mRNA Translation: BAG60589.1
AB209692 mRNA Translation: BAD92929.1 Different initiation.
AP001858 Genomic DNA No translation available.
BC022387 mRNA Translation: AAH22387.1
CCDSiCCDS53643.1 [Q8TCC7-5]
CCDS53644.1 [Q8TCC7-4]
CCDS8042.1 [Q8TCC7-1]
RefSeqiNP_001171661.1, NM_001184732.1 [Q8TCC7-1]
NP_001171662.1, NM_001184733.1 [Q8TCC7-4]
NP_001171665.1, NM_001184736.1 [Q8TCC7-5]
NP_004245.2, NM_004254.3 [Q8TCC7-1]
XP_011543666.1, XM_011545364.1 [Q8TCC7-5]
UniGeneiHs.266223

Genome annotation databases

EnsembliENST00000336232; ENSP00000337335; ENSG00000149452 [Q8TCC7-1]
ENST00000430500; ENSP00000398548; ENSG00000149452 [Q8TCC7-1]
ENST00000535878; ENSP00000443368; ENSG00000149452 [Q8TCC7-5]
ENST00000545207; ENSP00000441658; ENSG00000149452 [Q8TCC7-4]
GeneIDi9376
KEGGihsa:9376
UCSCiuc001nwo.4 human [Q8TCC7-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiS22A8_HUMAN
AccessioniPrimary (citable) accession number: Q8TCC7
Secondary accession number(s): B4DPH7
, F5GWA8, F5H5J1, O95820, Q59EW9, Q96TC1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: June 1, 2002
Last modified: February 28, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

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