Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8TC59 (PIWL2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Piwi-like protein 2
Alternative name(s):
Cancer/testis antigen 80
Short name=CT80
Gene names
Name:PIWIL2
Synonyms:HILI
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length973 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Its presence in oocytes suggests that it may participate in similar functions during oogenesis in females. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with primary piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. The piRNA process acts upstream of known mediators of DNA methylation. Participates in a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Indirectly modulate expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8. Inhibits tumor cell growth when repressed. When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors By similarity. Ref.5

Subunit structure

Interacts with DDX4, MAEL, EIF3A, EIF4E, EIF4G, PRMT5 and WDR77. Associates with EIF4E- and EIF4G-containing m7G cap-binding complexes. Interacts (when methylated on arginine residues) with TDRD1 and TDRKH/TDRD2. Interacts with TDRD12 By similarity.

Subcellular location

Cytoplasm By similarity. Note: Present in chromatoid body. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis By similarity.

Tissue specificity

Expressed in adult testis and in most tumors. Ref.5

Post-translational modification

Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein TDRD1 and subsequent localization to the meiotic nuage, also named P granule By similarity.

Sequence similarities

Belongs to the argonaute family. Piwi subfamily.

Contains 1 PAZ domain.

Contains 1 Piwi domain.

Sequence caution

The sequence BAA91558.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAB55155.1 differs from that shown. Reason: Erroneous termination at position 421. Translated as Arg.

Ontologies

Keywords
   Biological processDifferentiation
Meiosis
Oogenesis
RNA-mediated gene silencing
Spermatogenesis
Translation regulation
   Cellular componentCytoplasm
   Coding sequence diversityAlternative splicing
   LigandRNA-binding
   Molecular functionDevelopmental protein
   PTMMethylation
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA methylation involved in gamete generation

Inferred from sequence or structural similarity. Source: UniProtKB

RNA 5'-end processing

Inferred from sequence or structural similarity. Source: UniProtKB

gene silencing by RNA

Inferred from sequence or structural similarity. Source: UniProtKB

germ-line stem cell maintenance

Inferred from sequence or structural similarity. Source: UniProtKB

meiotic nuclear division

Inferred from electronic annotation. Source: UniProtKB-KW

multicellular organismal development

Inferred from electronic annotation. Source: UniProtKB-KW

oogenesis

Inferred from sequence or structural similarity. Source: UniProtKB

piRNA metabolic process

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of meiosis I

Inferred from electronic annotation. Source: Ensembl

positive regulation of translation

Inferred from sequence or structural similarity. Source: UniProtKB

spermatogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentP granule

Inferred from sequence or structural similarity. Source: UniProtKB

chromatoid body

Inferred from sequence or structural similarity. Source: UniProtKB

cytoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

pi-body

Inferred from sequence or structural similarity. Source: UniProtKB

polysome

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionmRNA binding

Inferred from electronic annotation. Source: Ensembl

piRNA binding

Inferred from direct assay Ref.6. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8TC59-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8TC59-2)

The sequence of this isoform differs from the canonical sequence as follows:
     887-922: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 973973Piwi-like protein 2
PRO_0000234569

Regions

Domain384 – 496113PAZ
Domain668 – 959292Piwi

Amino acid modifications

Modified residue471Symmetric dimethylarginine By similarity
Modified residue761Omega-N-methylarginine; by PRMT5; alternate By similarity
Modified residue761Symmetric dimethylarginine; by PRMT5; alternate By similarity
Modified residue971Omega-N-methylarginine; by PRMT5; alternate By similarity
Modified residue971Symmetric dimethylarginine; alternate By similarity
Modified residue1021Omega-N-methylarginine; alternate By similarity
Modified residue1021Symmetric dimethylarginine; by PRMT5; alternate By similarity
Modified residue1651Symmetric dimethylarginine; by PRMT5 By similarity
Modified residue5511Symmetric dimethylarginine; by PRMT5 By similarity

Natural variations

Alternative sequence887 – 92236Missing in isoform 2.
VSP_036664

Experimental info

Sequence conflict181Q → R in BAG61134. Ref.3
Sequence conflict5811I → T in BAF98721. Ref.3
Sequence conflict6851K → N in BAA91558. Ref.3
Sequence conflict7201Q → R in BAF98724. Ref.3
Sequence conflict8871V → G in BAF83727. Ref.3
Sequence conflict9611E → G in BAB55155. Ref.3

Secondary structure

......................... 973
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: C44398136B144CA0

FASTA973109,849
        10         20         30         40         50         60 
MDPFRPSFRG QSPIHPSQCQ AVRMPGCWPQ ASKPLDPALG RGAPAGRGHV FGKPEEPSTQ 

        70         80         90        100        110        120 
RGPAQRESVG LVSMFRGLGI ETVSKTPLKR EMLPSGRGIL GRGLSANLVR KDREELSPTF 

       130        140        150        160        170        180 
WDPKVLAAGD SKMAETSVGW SRTLGRGSSD ASLLPLGRAA GGISREVDKP PCTFSTPSRG 

       190        200        210        220        230        240 
PPQLSSPPAL PQSPLHSPDR PLVLTVEHKE KELIVKQGSK GTPQSLGLNL VKIQCHNEAV 

       250        260        270        280        290        300 
YQYHVTFSPN VECKSMRFGM LKDHQAVTGN VTAFDGSILY LPVKLQQVLE LKSQRKTDSA 

       310        320        330        340        350        360 
EISIKIQMTK ILEPCSDLCI PFYNVVFRRV MKLLDMKLVG RNFYDPTSAM VLQQHRLQIW 

       370        380        390        400        410        420 
PGYAASIRRT DGGLFLLADV SHKVIRNDCV LDVMHAIYQQ NKEHFQDECT KLLVGNIVIT 

       430        440        450        460        470        480 
RYNNRTYRID DVDWNKTPKD SFTMSDGKEI TFLEYYSKNY GITVKEEDQP LLIHRPSERQ 

       490        500        510        520        530        540 
DNHGMLLKGE ILLLPELSFM TGIPEKMKKD FRAMKDLAQQ INLSPKQHHS ALECLLQRIA 

       550        560        570        580        590        600 
KNEAATNELM RWGLRLQKDV HKIEGRVLPM ERINLKNTSF ITSQELNWVK EVTRDPSILT 

       610        620        630        640        650        660 
IPMHFWALFY PKRAMDQARE LVNMLEKIAG PIGMRMSPPA WVELKDDRIE TYVRTIQSTL 

       670        680        690        700        710        720 
GAEGKIQMVV CIIMGPRDDL YGAIKKLCCV QSPVPSQVVN VRTIGQPTRL RSVAQKILLQ 

       730        740        750        760        770        780 
INCKLGGELW GVDIPLKQLM VIGMDVYHDP SRGMRSVVGF VASINLTLTK WYSRVVFQMP 

       790        800        810        820        830        840 
HQEIVDSLKL CLVGSLKKFY EVNHCLPEKI VVYRDGVSDG QLKTVANYEI PQLQKCFEAF 

       850        860        870        880        890        900 
ENYQPKMVVF VVQKKISTNL YLAAPQNFVT PTPGTVVDHT ITSCEWVDFY LLAHHVRQGC 

       910        920        930        940        950        960 
GIPTHYVCVL NTANLSPDHM QRLTFKLCHM YWNWPGTIRV PAPCKYAHKL AFLSGHILHH 

       970 
EPAIQLCENL FFL 

« Hide

Isoform 2 [UniParc].

Checksum: 5888D266DD32416C
Show »

FASTA937105,760

References

« Hide 'large scale' references
[1]"Identification of eight members of the Argonaute family in the human genome."
Sasaki T., Shiohama A., Minoshima S., Shimizu N.
Genomics 82:323-330(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Identification of piwil2-like (PL2L106) protein in HeLa cells."
Zhang K., Lu Y., Li C.
Submitted (NOV-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Testis.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Testis.
[5]"Stem-cell protein Piwil2 is widely expressed in tumors and inhibits apoptosis through activation of Stat3/Bcl-XL pathway."
Lee J.H., Schutte D., Wulf G., Fuzesi L., Radzun H.-J., Schweyer S., Engel W., Nayernia K.
Hum. Mol. Genet. 15:201-211(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
[6]"Structural basis for piRNA 2'-O-methylated 3'-end recognition by Piwi PAZ (Piwi/Argonaute/Zwille) domains."
Tian Y., Simanshu D.K., Ma J.B., Patel D.J.
Proc. Natl. Acad. Sci. U.S.A. 108:903-910(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.92 ANGSTROMS) OF 387-525, RNA-BINDING.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB079367 mRNA. Translation: BAC81342.1.
HQ651229 mRNA. Translation: ADV17663.1.
AK001213 mRNA. Translation: BAA91558.1. Different initiation.
AK027497 mRNA. Translation: BAB55155.1. Sequence problems.
AK291038 mRNA. Translation: BAF83727.1.
AK292440 mRNA. Translation: BAF85129.1.
AK299068 mRNA. Translation: BAG61134.1.
AK315830 mRNA. Translation: BAF98721.1.
AK315833 mRNA. Translation: BAF98724.1.
BC025995 mRNA. Translation: AAH25995.1.
BC111751 mRNA. Translation: AAI11752.1.
CCDSCCDS6029.1. [Q8TC59-1]
RefSeqNP_001129193.1. NM_001135721.1. [Q8TC59-1]
NP_060538.2. NM_018068.3. [Q8TC59-1]
XP_005273607.1. XM_005273550.2. [Q8TC59-2]
UniGeneHs.614809.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3O7XX-ray2.92A/B/C/D387-525[»]
3QIRX-ray2.45A/B/C/D386-533[»]
ProteinModelPortalQ8TC59.
SMRQ8TC59. Positions 218-962.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120431. 2 interactions.
STRING9606.ENSP00000349208.

PTM databases

PhosphoSiteQ8TC59.

Polymorphism databases

DMDM74730558.

Proteomic databases

PaxDbQ8TC59.
PRIDEQ8TC59.

Protocols and materials databases

DNASU55124.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000356766; ENSP00000349208; ENSG00000197181. [Q8TC59-1]
ENST00000454009; ENSP00000406956; ENSG00000197181. [Q8TC59-1]
ENST00000521356; ENSP00000428267; ENSG00000197181. [Q8TC59-2]
GeneID55124.
KEGGhsa:55124.
UCSCuc003xbn.2. human. [Q8TC59-1]
uc011kzf.1. human. [Q8TC59-2]

Organism-specific databases

CTD55124.
GeneCardsGC08P022132.
HGNCHGNC:17644. PIWIL2.
HPAHPA029345.
MIM610312. gene.
neXtProtNX_Q8TC59.
PharmGKBPA38461.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG286051.
HOVERGENHBG049411.
InParanoidQ8TC59.
KOK02156.
OMAQELNWIK.
OrthoDBEOG712TVQ.
PhylomeDBQ8TC59.
TreeFamTF354206.

Gene expression databases

BgeeQ8TC59.
CleanExHS_PIWIL2.
GenevestigatorQ8TC59.

Family and domain databases

Gene3D3.30.420.10. 1 hit.
InterProIPR003100. PAZ_dom.
IPR003165. Piwi.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamPF02170. PAZ. 1 hit.
PF02171. Piwi. 1 hit.
[Graphical view]
SMARTSM00949. PAZ. 1 hit.
SM00950. Piwi. 1 hit.
[Graphical view]
SUPFAMSSF101690. SSF101690. 1 hit.
SSF53098. SSF53098. 1 hit.
PROSITEPS50821. PAZ. 1 hit.
PS50822. PIWI. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi55124.
NextBio58776.
PROQ8TC59.
SOURCESearch...

Entry information

Entry namePIWL2_HUMAN
AccessionPrimary (citable) accession number: Q8TC59
Secondary accession number(s): A8K4S3 expand/collapse secondary AC list , A8K8S5, B0AZN9, B0AZP2, B4DR22, E7ECA4, Q96SW6, Q9NW28
Entry history
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: June 1, 2002
Last modified: July 9, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 8

Human chromosome 8: entries, gene names and cross-references to MIM