Q8TC27 (ADA32_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Disintegrin and metalloproteinase domain-containing protein 32
Short name=ADAM 32

Gene names

Name:	ADAM32
ORF Names:	UNQ5982/PRO21340

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	787 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in sperm development and fertilization This is a non-catalytic metalloprotease-like protein By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein Potential.
Tissue specificity	Testis specific. Ref.5
Sequence similarities	Contains 1 disintegrin domain. Contains 1 EGF-like domain. Contains 1 peptidase M12B domain.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	EGF-like domain Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Ref.4
	Propeptide	17 – 174	158	Potential		PRO_0000029138
	Chain	175 – 787	613	Disintegrin and metalloproteinase domain-containing protein 32		PRO_0000029139
Regions
	Topological domain	175 – 682	508	Extracellular Potential
	Transmembrane	683 – 703	21	Helical; Potential
	Topological domain	704 – 787	84	Cytoplasmic Potential
	Domain	186 – 383	198	Peptidase M12B
	Domain	391 – 479	89	Disintegrin
	Domain	622 – 654	33	EGF-like
	Compositional bias	480 – 502	23	Cys-rich
Amino acid modifications
	Modified residue	17	1	Phosphoserine Ref.6 Ref.7
	Glycosylation	39	1	N-linked (GlcNAc...) Potential
	Glycosylation	125	1	N-linked (GlcNAc...) Potential
	Glycosylation	465	1	N-linked (GlcNAc...) Potential
	Glycosylation	598	1	N-linked (GlcNAc...) Potential
	Disulfide bond	295 ↔ 378		By similarity
	Disulfide bond	337 ↔ 362		By similarity
	Disulfide bond	339 ↔ 344		By similarity
	Disulfide bond	450 ↔ 471		By similarity
	Disulfide bond	626 ↔ 636		By similarity
	Disulfide bond	630 ↔ 642		By similarity
	Disulfide bond	644 ↔ 653		By similarity
Natural variations
	Natural variant	98	1	Q → R. Ref.3 Corresponds to variant rs17856744 [ dbSNP | Ensembl ].		VAR_055241
	Natural variant	160	1	S → G. Ref.3 Corresponds to variant rs17852343 [ dbSNP | Ensembl ].		VAR_055242
	Natural variant	327	1	L → V. Corresponds to variant rs9643859 [ dbSNP | Ensembl ].		VAR_055243
	Natural variant	467	1	T → S. Ref.1 Ref.3 Corresponds to variant rs7845771 [ dbSNP | Ensembl ].		VAR_051591
	Natural variant	658	1	K → N. Corresponds to variant rs13277386 [ dbSNP | Ensembl ].		VAR_055244
	Natural variant	778	1	D → E. Corresponds to variant rs28705715 [ dbSNP | Ensembl ].		VAR_061739

Sequences

	Sequence		Length	Mass (Da)
	Q8TC27 [UniParc]. Last modified May 18, 2010. Version 2. Checksum: 841EDA9EFF483A2C	FASTA	787	87,948
	10 20 30 40 50 60 MFRLWLLLAG LCGLLASRPG FQNSLLQIVI PEKIQTNTND SSEIEYEQIS YIIPIDEKLY 70 80 90 100 110 120 TVHLKQRYFL ADNFMIYLYN QGSMNTYSSD IQTQCYYQGN IEGYPDSMVT LSTCSGLRGI 130 140 150 160 170 180 LQFENVSYGI EPLESAVEFQ HVLYKLKNED NDIAIFIDRS LKEQPMDDNI FISEKSEPAV 190 200 210 220 230 240 PDLFPLYLEM HIVVDKTLYD YWGSDSMIVT NKVIEIVGLA NSMFTQFKVT IVLSSLELWS 250 260 270 280 290 300 DENKISTVGE ADELLQKFLE WKQSYLNLRP HDIAYLLIYM DYPRYLGAVF PGTMCITRYS 310 320 330 340 350 360 AGVALYPKEI TLEAFAVIVT QMLALSLGIS YDDPKKCQCS ESTCIMNPEV VQSNGVKTFS 370 380 390 400 410 420 SCSLRSFQNF ISNVGVKCLQ NKPQMQKKSP KPVCGNGRLE GNEICDCGTE AQCGPASCCD 430 440 450 460 470 480 FRTCVLKDGA KCYKGLCCKD CQILQSGVEC RPKAHPECDI AENCNGTSPE CGPDITLING 490 500 510 520 530 540 LSCKNNKFIC YDGDCHDLDA RCESVFGKGS RNAPFACYEE IQSQSDRFGN CGRDRNNKYV 550 560 570 580 590 600 FCGWRNLICG RLVCTYPTRK PFHQENGDVI YAFVRDSVCI TVDYKLPRTV PDPLAVKNGS 610 620 630 640 650 660 QCDIGRVCVN RECVESRIIK ASAHVCSQQC SGHGVCDSRN KCHCSPGYKP PNCQIRSKGF 670 680 690 700 710 720 SIFPEEDMGS IMERASGKTE NTWLLGFLIA LPILIVTTAI VLARKQLKKW FAKEEEFPSS 730 740 750 760 770 780 ESKSEGSTQT YASQSSSEGS TQTYASQTRS ESSSQADTSK SKSEDSAEAY TSRSKSQDST QTQSSSN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT SER-467.
[2]	"DNA sequence and analysis of human chromosome 8." Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S. Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS ARG-98; GLY-160 AND SER-467. Tissue: Testis.
[4]	"Signal peptide prediction based on analysis of experimentally verified cleavage sites." Zhang Z., Henzel W.J. Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 17-31.
[5]	"Identification and characterization of ADAM32 with testis-predominant gene expression." Choi I., Woo J.-M., Hong S., Jung Y.-K., Kim D.H., Cho C. Gene 304:151-162(2003) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[6]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY358739 mRNA. Translation: AAQ89099.1. AC105091 Genomic DNA. No translation available. AC105185 Genomic DNA. No translation available. BC026085 mRNA. Translation: AAH26085.1. BC026169 mRNA. Translation: AAH26169.1. BC028702 mRNA. Translation: AAH28702.1. BC030014 mRNA. Translation: AAH30014.1. BC030698 mRNA. Translation: AAH30698.1. BC034975 mRNA. Translation: AAH34975.1.
IPI	IPI00748886.
RefSeq	NP_659441.3. NM_145004.5.
UniGene	Hs.521545.
3D structure databases
ProteinModelPortal	Q8TC27.
SMR	Q8TC27. Positions 187-656.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000369238.
Protein family/group databases
MEROPS	M12.960.
PTM databases
PhosphoSite	Q8TC27.
Polymorphism databases
DMDM	296434389.
Proteomic databases
PaxDb	Q8TC27.
PRIDE	Q8TC27.
Protocols and materials databases
DNASU	203102.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000379907; ENSP00000369238; ENSG00000197140.
GeneID	203102.
KEGG	hsa:203102.
UCSC	uc003xmt.4. human.
Organism-specific databases
CTD	203102.
GeneCards	GC08P038965.
HGNC	HGNC:15479. ADAM32.
HPA	HPA044156.
neXtProt	NX_Q8TC27.
PharmGKB	PA134932610.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG245239.
HOGENOM	HOG000230883.
HOVERGEN	HBG103628.
InParanoid	Q8TC27.
KO	K16070.
OMA	DDPKKCQ.
Gene expression databases
ArrayExpress	Q8TC27.
Bgee	Q8TC27.
CleanEx	HS_ADAM32.
Genevestigator	Q8TC27.
GermOnline	ENSG00000197140. Homo sapiens.
Family and domain databases
Gene3D	3.40.390.10. 1 hit. 4.10.70.10. 1 hit.
InterPro	IPR006586. ADAM_Cys-rich. IPR001762. Blood-coag_inhib_Disintegrin. IPR018358. Disintegrin_CS. IPR000742. EG-like_dom. IPR013032. EGF-like_CS. IPR024079. MetalloPept_cat_dom. IPR001590. Peptidase_M12B. IPR002870. Peptidase_M12B_N. [Graphical view]
Pfam	PF08516. ADAM_CR. 1 hit. PF00200. Disintegrin. 1 hit. PF01562. Pep_M12B_propep. 1 hit. PF01421. Reprolysin. 1 hit. [Graphical view]
SMART	SM00608. ACR. 1 hit. SM00050. DISIN. 1 hit. SM00181. EGF. 1 hit. [Graphical view]
SUPFAM	SSF57552. Disintegrin. 1 hit.
PROSITE	PS50215. ADAM_MEPRO. 1 hit. PS00427. DISINTEGRIN_1. 1 hit. PS50214. DISINTEGRIN_2. 1 hit. PS00022. EGF_1. False negative. PS01186. EGF_2. 1 hit. PS50026. EGF_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	203102.
NextBio	90349.

Entry information

Entry name

ADA32_HUMAN

Accession

Primary (citable) accession number: Q8TC27
Secondary accession number(s): Q8TC42

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
Last sequence update:	May 18, 2010
Last modified:	May 1, 2013
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.