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Protein

Disintegrin and metalloproteinase domain-containing protein 32

Gene

ADAM32

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in sperm development and fertilization This is a non-catalytic metalloprotease-like protein.By similarity

GO - Molecular functioni

  1. metalloendopeptidase activity Source: InterPro
  2. zinc ion binding Source: InterPro
Complete GO annotation...

Protein family/group databases

MEROPSiM12.953.

Names & Taxonomyi

Protein namesi
Recommended name:
Disintegrin and metalloproteinase domain-containing protein 32
Short name:
ADAM 32
Gene namesi
Name:ADAM32
ORF Names:UNQ5982/PRO21340
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 8, UP000005640: Unplaced

Organism-specific databases

HGNCiHGNC:15479. ADAM32.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini175 – 682508ExtracellularSequence AnalysisAdd
BLAST
Transmembranei683 – 70321HelicalSequence AnalysisAdd
BLAST
Topological domaini704 – 78784CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134932610.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 16161 PublicationAdd
BLAST
Propeptidei17 – 174158Sequence AnalysisPRO_0000029138Add
BLAST
Chaini175 – 787613Disintegrin and metalloproteinase domain-containing protein 32PRO_0000029139Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei17 – 171Phosphoserine2 Publications
Glycosylationi39 – 391N-linked (GlcNAc...)Sequence Analysis
Glycosylationi125 – 1251N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi295 ↔ 378By similarity
Disulfide bondi337 ↔ 362By similarity
Disulfide bondi339 ↔ 344By similarity
Disulfide bondi450 ↔ 471By similarity
Glycosylationi465 – 4651N-linked (GlcNAc...)Sequence Analysis
Glycosylationi598 – 5981N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi626 ↔ 636By similarity
Disulfide bondi630 ↔ 642By similarity
Disulfide bondi644 ↔ 653By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ8TC27.
PRIDEiQ8TC27.

PTM databases

PhosphoSiteiQ8TC27.

Expressioni

Tissue specificityi

Testis specific.1 Publication

Gene expression databases

BgeeiQ8TC27.
CleanExiHS_ADAM32.
ExpressionAtlasiQ8TC27. baseline and differential.
GenevestigatoriQ8TC27.

Organism-specific databases

HPAiHPA044156.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000369238.

Structurei

3D structure databases

ProteinModelPortaliQ8TC27.
SMRiQ8TC27. Positions 187-653.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini186 – 383198Peptidase M12BPROSITE-ProRule annotationAdd
BLAST
Domaini391 – 47989DisintegrinPROSITE-ProRule annotationAdd
BLAST
Domaini622 – 65433EGF-likePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi480 – 50223Cys-richAdd
BLAST

Sequence similaritiesi

Contains 1 disintegrin domain.PROSITE-ProRule annotation
Contains 1 EGF-like domain.PROSITE-ProRule annotation
Contains 1 peptidase M12B domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG245239.
GeneTreeiENSGT00760000119181.
HOGENOMiHOG000230883.
HOVERGENiHBG103628.
InParanoidiQ8TC27.
KOiK16070.
OMAiFPLYLEM.
PhylomeDBiQ8TC27.
TreeFamiTF314733.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiIPR006586. ADAM_Cys-rich.
IPR001762. Blood-coag_inhib_Disintegrin.
IPR018358. Disintegrin_CS.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PfamiPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
SMARTiSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
SM00181. EGF. 1 hit.
[Graphical view]
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8TC27-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFRLWLLLAG LCGLLASRPG FQNSLLQIVI PEKIQTNTND SSEIEYEQIS
60 70 80 90 100
YIIPIDEKLY TVHLKQRYFL ADNFMIYLYN QGSMNTYSSD IQTQCYYQGN
110 120 130 140 150
IEGYPDSMVT LSTCSGLRGI LQFENVSYGI EPLESAVEFQ HVLYKLKNED
160 170 180 190 200
NDIAIFIDRS LKEQPMDDNI FISEKSEPAV PDLFPLYLEM HIVVDKTLYD
210 220 230 240 250
YWGSDSMIVT NKVIEIVGLA NSMFTQFKVT IVLSSLELWS DENKISTVGE
260 270 280 290 300
ADELLQKFLE WKQSYLNLRP HDIAYLLIYM DYPRYLGAVF PGTMCITRYS
310 320 330 340 350
AGVALYPKEI TLEAFAVIVT QMLALSLGIS YDDPKKCQCS ESTCIMNPEV
360 370 380 390 400
VQSNGVKTFS SCSLRSFQNF ISNVGVKCLQ NKPQMQKKSP KPVCGNGRLE
410 420 430 440 450
GNEICDCGTE AQCGPASCCD FRTCVLKDGA KCYKGLCCKD CQILQSGVEC
460 470 480 490 500
RPKAHPECDI AENCNGTSPE CGPDITLING LSCKNNKFIC YDGDCHDLDA
510 520 530 540 550
RCESVFGKGS RNAPFACYEE IQSQSDRFGN CGRDRNNKYV FCGWRNLICG
560 570 580 590 600
RLVCTYPTRK PFHQENGDVI YAFVRDSVCI TVDYKLPRTV PDPLAVKNGS
610 620 630 640 650
QCDIGRVCVN RECVESRIIK ASAHVCSQQC SGHGVCDSRN KCHCSPGYKP
660 670 680 690 700
PNCQIRSKGF SIFPEEDMGS IMERASGKTE NTWLLGFLIA LPILIVTTAI
710 720 730 740 750
VLARKQLKKW FAKEEEFPSS ESKSEGSTQT YASQSSSEGS TQTYASQTRS
760 770 780
ESSSQADTSK SKSEDSAEAY TSRSKSQDST QTQSSSN
Length:787
Mass (Da):87,948
Last modified:May 18, 2010 - v2
Checksum:i841EDA9EFF483A2C
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti98 – 981Q → R.1 Publication
Corresponds to variant rs17856744 [ dbSNP | Ensembl ].
VAR_055241
Natural varianti160 – 1601S → G.1 Publication
Corresponds to variant rs17852343 [ dbSNP | Ensembl ].
VAR_055242
Natural varianti327 – 3271L → V.
Corresponds to variant rs9643859 [ dbSNP | Ensembl ].
VAR_055243
Natural varianti467 – 4671T → S.2 Publications
Corresponds to variant rs7845771 [ dbSNP | Ensembl ].
VAR_051591
Natural varianti658 – 6581K → N.
Corresponds to variant rs13277386 [ dbSNP | Ensembl ].
VAR_055244
Natural varianti778 – 7781D → E.
Corresponds to variant rs28705715 [ dbSNP | Ensembl ].
VAR_061739

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY358739 mRNA. Translation: AAQ89099.1.
AC105091 Genomic DNA. No translation available.
AC105185 Genomic DNA. No translation available.
BC026085 mRNA. Translation: AAH26085.1.
BC026169 mRNA. Translation: AAH26169.1.
BC028702 mRNA. Translation: AAH28702.1.
BC030014 mRNA. Translation: AAH30014.1.
BC030698 mRNA. Translation: AAH30698.1.
BC034975 mRNA. Translation: AAH34975.1.
CCDSiCCDS47846.1.
RefSeqiNP_659441.3. NM_145004.5.
UniGeneiHs.521545.

Genome annotation databases

EnsembliENST00000379907; ENSP00000369238; ENSG00000197140.
ENST00000615420; ENSP00000484817; ENSG00000275594.
GeneIDi203102.
KEGGihsa:203102.
UCSCiuc003xmt.4. human.

Polymorphism databases

DMDMi296434389.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY358739 mRNA. Translation: AAQ89099.1.
AC105091 Genomic DNA. No translation available.
AC105185 Genomic DNA. No translation available.
BC026085 mRNA. Translation: AAH26085.1.
BC026169 mRNA. Translation: AAH26169.1.
BC028702 mRNA. Translation: AAH28702.1.
BC030014 mRNA. Translation: AAH30014.1.
BC030698 mRNA. Translation: AAH30698.1.
BC034975 mRNA. Translation: AAH34975.1.
CCDSiCCDS47846.1.
RefSeqiNP_659441.3. NM_145004.5.
UniGeneiHs.521545.

3D structure databases

ProteinModelPortaliQ8TC27.
SMRiQ8TC27. Positions 187-653.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000369238.

Protein family/group databases

MEROPSiM12.953.

PTM databases

PhosphoSiteiQ8TC27.

Polymorphism databases

DMDMi296434389.

Proteomic databases

PaxDbiQ8TC27.
PRIDEiQ8TC27.

Protocols and materials databases

DNASUi203102.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000379907; ENSP00000369238; ENSG00000197140.
ENST00000615420; ENSP00000484817; ENSG00000275594.
GeneIDi203102.
KEGGihsa:203102.
UCSCiuc003xmt.4. human.

Organism-specific databases

CTDi203102.
GeneCardsiGC08P038965.
HGNCiHGNC:15479. ADAM32.
HPAiHPA044156.
neXtProtiNX_Q8TC27.
PharmGKBiPA134932610.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG245239.
GeneTreeiENSGT00760000119181.
HOGENOMiHOG000230883.
HOVERGENiHBG103628.
InParanoidiQ8TC27.
KOiK16070.
OMAiFPLYLEM.
PhylomeDBiQ8TC27.
TreeFamiTF314733.

Miscellaneous databases

GenomeRNAii203102.
NextBioi90349.
PROiQ8TC27.

Gene expression databases

BgeeiQ8TC27.
CleanExiHS_ADAM32.
ExpressionAtlasiQ8TC27. baseline and differential.
GenevestigatoriQ8TC27.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiIPR006586. ADAM_Cys-rich.
IPR001762. Blood-coag_inhib_Disintegrin.
IPR018358. Disintegrin_CS.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PfamiPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
SMARTiSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
SM00181. EGF. 1 hit.
[Graphical view]
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT SER-467.
  2. "DNA sequence and analysis of human chromosome 8."
    Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T.
    , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
    Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS ARG-98; GLY-160 AND SER-467.
    Tissue: Testis.
  4. "Signal peptide prediction based on analysis of experimentally verified cleavage sites."
    Zhang Z., Henzel W.J.
    Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 17-31.
  5. "Identification and characterization of ADAM32 with testis-predominant gene expression."
    Choi I., Woo J.-M., Hong S., Jung Y.-K., Kim D.H., Cho C.
    Gene 304:151-162(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  6. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
    Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
    Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.

Entry informationi

Entry nameiADA32_HUMAN
AccessioniPrimary (citable) accession number: Q8TC27
Secondary accession number(s): Q8TC42
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: May 18, 2010
Last modified: January 7, 2015
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. Peptidase families
    Classification of peptidase families and list of entries
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.