Reviewed, UniProtKB/Swiss-Prot Q8TC27 (ADA32_HUMAN)

Names and origin

Protein names

Recommended name:
    Disintegrin and metalloproteinase domain-containing protein 32
      Short name=ADAM 32

Gene names

Name:	ADAM32
ORF Names:	UNQ5982/PRO21340

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	787 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May play a role in sperm development and fertilization This is a non-catalytic metalloprotease-like protein By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein Potential.
Tissue specificity	Testis specific. Ref.5
Sequence similarities	Contains 1 disintegrin domain. Contains 1 EGF-like domain. Contains 1 peptidase M12B domain.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	EGF-like domain Signal Transmembrane
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Ref.4
	Propeptide	17 – 174	158	Potential		PRO_0000029138
	Chain	175 – 787	613	Disintegrin and metalloproteinase domain-containing protein 32		PRO_0000029139
Regions
	Topological domain	175 – 682	508	Extracellular Potential
	Transmembrane	683 – 703	21	Potential
	Topological domain	704 – 787	84	Cytoplasmic Potential
	Domain	186 – 383	198	Peptidase M12B
	Domain	391 – 479	89	Disintegrin
	Domain	622 – 654	33	EGF-like
	Compositional bias	480 – 502	23	Cys-rich
Amino acid modifications
	Modified residue	17	1	Phosphoserine Ref.6
	Glycosylation	39	1	N-linked (GlcNAc...) Potential
	Glycosylation	125	1	N-linked (GlcNAc...) Potential
	Glycosylation	465	1	N-linked (GlcNAc...) Potential
	Glycosylation	598	1	N-linked (GlcNAc...) Potential
	Disulfide bond	295 ↔ 378		By similarity
	Disulfide bond	337 ↔ 362		By similarity
	Disulfide bond	339 ↔ 344		By similarity
	Disulfide bond	450 ↔ 471		By similarity
	Disulfide bond	626 ↔ 636		By similarity
	Disulfide bond	630 ↔ 642		By similarity
	Disulfide bond	644 ↔ 653		By similarity
Natural variations
	Natural variant	98	1	Q → R: dbSNP rs17856744. Ref.3		VAR_055241
	Natural variant	160	1	S → G: dbSNP rs17852343. Ref.3		VAR_055242
	Natural variant	327	1	L → V: dbSNP rs9643859.		VAR_055243
	Natural variant	467	1	S → T: dbSNP rs7845771.		VAR_051591
	Natural variant	658	1	K → N: dbSNP rs13277386.		VAR_055244

Sequences

	Sequence		Length	Mass (Da)
	Q8TC27-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 3D84CACFECC1A12E	FASTA	787	87,934
	10 20 30 40 50 60 MFRLWLLLAG LCGLLASRPG FQNSLLQIVI PEKIQTNTND SSEIEYEQIS YIIPIDEKLY 70 80 90 100 110 120 TVHLKQRYFL ADNFMIYLYN QGSMNTYSSD IQTQCYYQGN IEGYPDSMVT LSTCSGLRGI 130 140 150 160 170 180 LQFENVSYGI EPLESAVEFQ HVLYKLKNED NDIAIFIDRS LKEQPMDDNI FISEKSEPAV 190 200 210 220 230 240 PDLFPLYLEM HIVVDKTLYD YWGSDSMIVT NKVIEIVGLA NSMFTQFKVT IVLSSLELWS 250 260 270 280 290 300 DENKISTVGE ADELLQKFLE WKQSYLNLRP HDIAYLLIYM DYPRYLGAVF PGTMCITRYS 310 320 330 340 350 360 AGVALYPKEI TLEAFAVIVT QMLALSLGIS YDDPKKCQCS ESTCIMNPEV VQSNGVKTFS 370 380 390 400 410 420 SCSLRSFQNF ISNVGVKCLQ NKPQMQKKSP KPVCGNGRLE GNEICDCGTE AQCGPASCCD 430 440 450 460 470 480 FRTCVLKDGA KCYKGLCCKD CQILQSGVEC RPKAHPECDI AENCNGSSPE CGPDITLING 490 500 510 520 530 540 LSCKNNKFIC YDGDCHDLDA RCESVFGKGS RNAPFACYEE IQSQSDRFGN CGRDRNNKYV 550 560 570 580 590 600 FCGWRNLICG RLVCTYPTRK PFHQENGDVI YAFVRDSVCI TVDYKLPRTV PDPLAVKNGS 610 620 630 640 650 660 QCDIGRVCVN RECVESRIIK ASAHVCSQQC SGHGVCDSRN KCHCSPGYKP PNCQIRSKGF 670 680 690 700 710 720 SIFPEEDMGS IMERASGKTE NTWLLGFLIA LPILIVTTAI VLARKQLKKW FAKEEEFPSS 730 740 750 760 770 780 ESKSEGSTQT YASQSSSEGS TQTYASQTRS ESSSQADTSK SKSEDSAEAY TSRSKSQDST QTQSSSN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	"DNA sequence and analysis of human chromosome 8." Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S. Nature 439:331-335(2006) [PubMed: 16421571] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS ARG-98 AND GLY-160. Tissue: Testis.
[4]	"Signal peptide prediction based on analysis of experimentally verified cleavage sites." Zhang Z., Henzel W.J. Protein Sci. 13:2819-2824(2004) [PubMed: 15340161] [Abstract] Cited for: PROTEIN SEQUENCE OF 17-31.
[5]	"Identification and characterization of ADAM32 with testis-predominant gene expression." Choi I., Woo J.-M., Hong S., Jung Y.-K., Kim D.H., Cho C. Gene 304:151-162(2003) [PubMed: 12568724] [Abstract] Cited for: TISSUE SPECIFICITY.
[6]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17, MASS SPECTROMETRY. Tissue: Epithelium.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY358739 mRNA. Translation: AAQ89099.1. AC105091 Genomic DNA. No translation available. AC105185 Genomic DNA. No translation available. BC026085 mRNA. Translation: AAH26085.1. BC026169 mRNA. Translation: AAH26169.1. BC028702 mRNA. Translation: AAH28702.1. BC030014 mRNA. Translation: AAH30014.1. BC030698 mRNA. Translation: AAH30698.1. BC034975 mRNA. Translation: AAH34975.1.
IPI	IPI00748886.
RefSeq	NP_659441.3.
UniGene	Hs.521545
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	M12.960.
PTM databases
PhosphoSite	Q8TC27.
Genome annotation databases
Ensembl	ENST00000379907; ENSP00000369238; ENSG00000197140; Homo sapiens. [Genome view]
GeneID	203102.
KEGG	hsa:203102.
UCSC	uc003xmt.2. human.
Organism-specific databases
CTD	203102.
GeneCards	GC08P039084.
H-InvDB	HIX0007466.
HGNC	HGNC:15479. ADAM32.
PharmGKB	PA134932610.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q8TC27.
HOVERGEN	Q8TC27.
Gene expression databases
ArrayExpress	Q8TC27.
Bgee	Q8TC27.
CleanEx	HS_ADAM32.
Genevestigator	Q8TC27.
GermOnline	ENSG00000197140. Homo sapiens.
Family and domain databases
InterPro	IPR006586. ADAM_Cys-rich. IPR001762. Blood-coag_inhib_Disintegrin. IPR018358. Disintegrin_CS. IPR006210. EGF-like. IPR013032. EGF-like_reg_CS. IPR000742. EGF_3. IPR013111. EGF_extracell. IPR001590. Peptidase_M12B. IPR002870. Peptidase_M12B_N. [Graphical view]
Gene3D	G3DSA:4.10.70.10. Blood-coag_inhib_Disintegrin. 1 hit.
Pfam	PF08516. ADAM_CR. 1 hit. PF00200. Disintegrin. 1 hit. PF07974. EGF_2. 1 hit. PF01562. Pep_M12B_propep. 1 hit. PF01421. Reprolysin. 1 hit. [Graphical view]
SMART	SM00608. ACR. 1 hit. SM00050. DISIN. 1 hit. SM00181. EGF. 1 hit. [Graphical view]
PROSITE	PS50215. ADAM_MEPRO. 1 hit. PS00427. DISINTEGRIN_1. 1 hit. PS50214. DISINTEGRIN_2. 1 hit. PS00022. EGF_1. False negative. PS01186. EGF_2. 1 hit. PS50026. EGF_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	90349.

Entry information

Entry name

ADA32_HUMAN

Accession

Primary (citable) accession number: Q8TC27
Secondary accession number(s): Q8TC42

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
Last sequence update:	June 1, 2002
Last modified:	November 24, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.