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Q8TC27 (ADA32_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Disintegrin and metalloproteinase domain-containing protein 32

Short name=ADAM 32
Gene names
Name:ADAM32
ORF Names:UNQ5982/PRO21340
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length787 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May play a role in sperm development and fertilization This is a non-catalytic metalloprotease-like protein By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein Potential.

Tissue specificity

Testis specific. Ref.5

Sequence similarities

Contains 1 disintegrin domain.

Contains 1 EGF-like domain.

Contains 1 peptidase M12B domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1616 Ref.4
Propeptide17 – 174158 Potential
PRO_0000029138
Chain175 – 787613Disintegrin and metalloproteinase domain-containing protein 32
PRO_0000029139

Regions

Topological domain175 – 682508Extracellular Potential
Transmembrane683 – 70321Helical; Potential
Topological domain704 – 78784Cytoplasmic Potential
Domain186 – 383198Peptidase M12B
Domain391 – 47989Disintegrin
Domain622 – 65433EGF-like
Compositional bias480 – 50223Cys-rich

Amino acid modifications

Modified residue171Phosphoserine Ref.6 Ref.7
Glycosylation391N-linked (GlcNAc...) Potential
Glycosylation1251N-linked (GlcNAc...) Potential
Glycosylation4651N-linked (GlcNAc...) Potential
Glycosylation5981N-linked (GlcNAc...) Potential
Disulfide bond295 ↔ 378 By similarity
Disulfide bond337 ↔ 362 By similarity
Disulfide bond339 ↔ 344 By similarity
Disulfide bond450 ↔ 471 By similarity
Disulfide bond626 ↔ 636 By similarity
Disulfide bond630 ↔ 642 By similarity
Disulfide bond644 ↔ 653 By similarity

Natural variations

Natural variant981Q → R. Ref.3
Corresponds to variant rs17856744 [ dbSNP | Ensembl ].
VAR_055241
Natural variant1601S → G. Ref.3
Corresponds to variant rs17852343 [ dbSNP | Ensembl ].
VAR_055242
Natural variant3271L → V.
Corresponds to variant rs9643859 [ dbSNP | Ensembl ].
VAR_055243
Natural variant4671T → S. Ref.1 Ref.3
Corresponds to variant rs7845771 [ dbSNP | Ensembl ].
VAR_051591
Natural variant6581K → N.
Corresponds to variant rs13277386 [ dbSNP | Ensembl ].
VAR_055244
Natural variant7781D → E.
Corresponds to variant rs28705715 [ dbSNP | Ensembl ].
VAR_061739

Sequences

Sequence LengthMass (Da)Tools
Q8TC27 [UniParc].

Last modified May 18, 2010. Version 2.
Checksum: 841EDA9EFF483A2C

FASTA78787,948
        10         20         30         40         50         60 
MFRLWLLLAG LCGLLASRPG FQNSLLQIVI PEKIQTNTND SSEIEYEQIS YIIPIDEKLY 

        70         80         90        100        110        120 
TVHLKQRYFL ADNFMIYLYN QGSMNTYSSD IQTQCYYQGN IEGYPDSMVT LSTCSGLRGI 

       130        140        150        160        170        180 
LQFENVSYGI EPLESAVEFQ HVLYKLKNED NDIAIFIDRS LKEQPMDDNI FISEKSEPAV 

       190        200        210        220        230        240 
PDLFPLYLEM HIVVDKTLYD YWGSDSMIVT NKVIEIVGLA NSMFTQFKVT IVLSSLELWS 

       250        260        270        280        290        300 
DENKISTVGE ADELLQKFLE WKQSYLNLRP HDIAYLLIYM DYPRYLGAVF PGTMCITRYS 

       310        320        330        340        350        360 
AGVALYPKEI TLEAFAVIVT QMLALSLGIS YDDPKKCQCS ESTCIMNPEV VQSNGVKTFS 

       370        380        390        400        410        420 
SCSLRSFQNF ISNVGVKCLQ NKPQMQKKSP KPVCGNGRLE GNEICDCGTE AQCGPASCCD 

       430        440        450        460        470        480 
FRTCVLKDGA KCYKGLCCKD CQILQSGVEC RPKAHPECDI AENCNGTSPE CGPDITLING 

       490        500        510        520        530        540 
LSCKNNKFIC YDGDCHDLDA RCESVFGKGS RNAPFACYEE IQSQSDRFGN CGRDRNNKYV 

       550        560        570        580        590        600 
FCGWRNLICG RLVCTYPTRK PFHQENGDVI YAFVRDSVCI TVDYKLPRTV PDPLAVKNGS 

       610        620        630        640        650        660 
QCDIGRVCVN RECVESRIIK ASAHVCSQQC SGHGVCDSRN KCHCSPGYKP PNCQIRSKGF 

       670        680        690        700        710        720 
SIFPEEDMGS IMERASGKTE NTWLLGFLIA LPILIVTTAI VLARKQLKKW FAKEEEFPSS 

       730        740        750        760        770        780 
ESKSEGSTQT YASQSSSEGS TQTYASQTRS ESSSQADTSK SKSEDSAEAY TSRSKSQDST 


QTQSSSN 

« Hide

References

« Hide 'large scale' references
[1]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT SER-467.
[2]"DNA sequence and analysis of human chromosome 8."
Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. expand/collapse author list , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS ARG-98; GLY-160 AND SER-467.
Tissue: Testis.
[4]"Signal peptide prediction based on analysis of experimentally verified cleavage sites."
Zhang Z., Henzel W.J.
Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 17-31.
[5]"Identification and characterization of ADAM32 with testis-predominant gene expression."
Choi I., Woo J.-M., Hong S., Jung Y.-K., Kim D.H., Cho C.
Gene 304:151-162(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[6]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[7]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY358739 mRNA. Translation: AAQ89099.1.
AC105091 Genomic DNA. No translation available.
AC105185 Genomic DNA. No translation available.
BC026085 mRNA. Translation: AAH26085.1.
BC026169 mRNA. Translation: AAH26169.1.
BC028702 mRNA. Translation: AAH28702.1.
BC030014 mRNA. Translation: AAH30014.1.
BC030698 mRNA. Translation: AAH30698.1.
BC034975 mRNA. Translation: AAH34975.1.
CCDSCCDS47846.1.
RefSeqNP_659441.3. NM_145004.5.
UniGeneHs.521545.

3D structure databases

ProteinModelPortalQ8TC27.
SMRQ8TC27. Positions 187-653.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000369238.

Protein family/group databases

MEROPSM12.960.

PTM databases

PhosphoSiteQ8TC27.

Polymorphism databases

DMDM296434389.

Proteomic databases

PaxDbQ8TC27.
PRIDEQ8TC27.

Protocols and materials databases

DNASU203102.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000379907; ENSP00000369238; ENSG00000197140.
GeneID203102.
KEGGhsa:203102.
UCSCuc003xmt.4. human.

Organism-specific databases

CTD203102.
GeneCardsGC08P038965.
HGNCHGNC:15479. ADAM32.
HPAHPA044156.
neXtProtNX_Q8TC27.
PharmGKBPA134932610.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG245239.
HOGENOMHOG000230883.
HOVERGENHBG103628.
InParanoidQ8TC27.
KOK16070.
OMAFPLYLEM.
PhylomeDBQ8TC27.
TreeFamTF314733.

Gene expression databases

ArrayExpressQ8TC27.
BgeeQ8TC27.
CleanExHS_ADAM32.
GenevestigatorQ8TC27.

Family and domain databases

Gene3D3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProIPR006586. ADAM_Cys-rich.
IPR001762. Blood-coag_inhib_Disintegrin.
IPR018358. Disintegrin_CS.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PfamPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
SMARTSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
SM00181. EGF. 1 hit.
[Graphical view]
SUPFAMSSF57552. SSF57552. 1 hit.
PROSITEPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi203102.
NextBio90349.
PROQ8TC27.

Entry information

Entry nameADA32_HUMAN
AccessionPrimary (citable) accession number: Q8TC27
Secondary accession number(s): Q8TC42
Entry history
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: May 18, 2010
Last modified: July 9, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 8

Human chromosome 8: entries, gene names and cross-references to MIM