Reviewed, UniProtKB/Swiss-Prot Q8TC20 (CAGE1_HUMAN)

Names and origin

Protein names

Recommended name:
    Cancer-associated gene 1 protein
      Short name=CAGE-1
Alternative name(s):
    Cancer/testis antigen 3
      Short name=CT3

Gene names

Name:	CAGE1
Synonyms:	CTAG3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	777 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Tissue specificity	Testis-specific expression in normal tissues, but wide expression among cancer tissues and cell lines.
Sequence caution	The sequence CAI16451.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil
Gene Ontology (GO)
None. [Check GOA]

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8TC20-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8TC20-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-136: Missing.

Isoform 3 (identifier: Q8TC20-3)

The sequence of this isoform differs from the canonical sequence as follows:
     668-668: E → ELLKHKDRITTFRELIAKEKAFQDHAIK
     765-777: MPALFKENRNDLD → LAYNLYIFIGYRLGAVADACNPSTLGGRGGWIT

Isoform 4 (identifier: Q8TC20-4)

The sequence of this isoform differs from the canonical sequence as follows:
     669-777: VIDCDSDEAK...LFKENRNDLD → LLKHKDRITTFRELIAKEKAFQDHAIKVFQGV

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 777	777	Cancer-associated gene 1 protein		PRO_0000280750
Regions
	Coiled coil	303 – 560	258	Potential
Natural variations
	Alternative sequence	1 – 136	136	Missing in isoform 2.		VSP_023902
	Alternative sequence	668	1	E → ELLKHKDRITTFRELIAKEK AFQDHAIK in isoform 3.		VSP_023969
	Alternative sequence	669 – 777	109	VIDCD…RNDLD → LLKHKDRITTFRELIAKEKA FQDHAIKVFQGV in isoform 4.		VSP_023971
	Alternative sequence	765 – 777	13	MPALF…RNDLD → LAYNLYIFIGYRLGAVADAC NPSTLGGRGGWIT in isoform 3.		VSP_023970
	Natural variant	169	1	T → I: dbSNP rs10223538.		VAR_031200
	Natural variant	282	1	E → A: dbSNP rs2876098.		VAR_031201
Experimental info
	Sequence conflict	20	1	V → A in BAC05162. Ref.2
	Sequence conflict	196	1	C → R in BAC05162. Ref.2
	Sequence conflict	596	1	S → P in BAC05162. Ref.2
	Sequence conflict	667	1	K → E in BAC05162. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 20, 2007. Version 2. Checksum: F69E220ADD5572EA	FASTA	777	90,250
	10 20 30 40 50 60 MNKDYQKFWS SPSDPVHFEV DTSHEKVESM SESDTMNVSN LSQGVMLSHS PICMETTGTT 70 80 90 100 110 120 CDLPQNEIKN FERENEYEST LCEDAYGTLD NLLNDNNIEN YSTNALIQPV DTISISSLRQ 130 140 150 160 170 180 FETVCKFHWV EAFDDEMTEK PEFQSQVYNY AKDNNIKQDS FKEENPMETS VSANTDQLGN 190 200 210 220 230 240 EYFRQPPPRS PPLIHCSGEM LKFTEKSLAK SIAKESALNP SQPPSFLCKT AVPSKEIQNY 250 260 270 280 290 300 GEIPEMSVSY EKEVTAEGVE RPEIVSTWSS AGISWRSEAC RENCEMPDWE QSAESLQPVQ 310 320 330 340 350 360 EDMALNEVLQ KLKHTNRKQE VRIQELQCSN LYLEKRVKEL QMKITKQQVF IDVINKLKEN 370 380 390 400 410 420 VEELIEDKYK IILEKNDTKK TLQNLEEVLA NTQKHLQESR NDKEMLQLQF KKIKANYVCL 430 440 450 460 470 480 QERYMTEMQQ KNKSVSQYLE MDKTLSKKEE EVERLQQLKK ELEKATASAL DLLKREKEAQ 490 500 510 520 530 540 EQEFLSLQEE FQKLEKENLE ERQKLKSRLE KLLTQVRNLQ FMSENERTKN IKLQQQINEV 550 560 570 580 590 600 KNENAKLKQQ VARSEEQNYV PKFETAQLKD QLEEVLKSDI TKDTKTTHSN LLPDCSPCEE 610 620 630 640 650 660 RLNPADIKRA SQLASKMHSL LALMVGLLTC QDIINSDAEH FKESEKVSDI MLQKLKSLHL 670 680 690 700 710 720 KKKTLDKEVI DCDSDEAKSI RDVPTLLGAK LDKYHSLNEE LDFLVTSYEE IIECADQRLA 730 740 750 760 770 ISHSQIAHLE ERNKHLEDLI RKPREKARKP RSKSLENHPK SMTMMPALFK ENRNDLD
	Isoform 2. Checksum: D3F479E4294C1B0A	FASTA	641	74,693
	10 20 30 40 50 60 MTEKPEFQSQ VYNYAKDNNI KQDSFKEENP METSVSANTD QLGNEYFRQP PPRSPPLIHC 70 80 90 100 110 120 SGEMLKFTEK SLAKSIAKES ALNPSQPPSF LCKTAVPSKE IQNYGEIPEM SVSYEKEVTA 130 140 150 160 170 180 EGVERPEIVS TWSSAGISWR SEACRENCEM PDWEQSAESL QPVQEDMALN EVLQKLKHTN 190 200 210 220 230 240 RKQEVRIQEL QCSNLYLEKR VKELQMKITK QQVFIDVINK LKENVEELIE DKYKIILEKN 250 260 270 280 290 300 DTKKTLQNLE EVLANTQKHL QESRNDKEML QLQFKKIKAN YVCLQERYMT EMQQKNKSVS 310 320 330 340 350 360 QYLEMDKTLS KKEEEVERLQ QLKKELEKAT ASALDLLKRE KEAQEQEFLS LQEEFQKLEK 370 380 390 400 410 420 ENLEERQKLK SRLEKLLTQV RNLQFMSENE RTKNIKLQQQ INEVKNENAK LKQQVARSEE 430 440 450 460 470 480 QNYVPKFETA QLKDQLEEVL KSDITKDTKT THSNLLPDCS PCEERLNPAD IKRASQLASK 490 500 510 520 530 540 MHSLLALMVG LLTCQDIINS DAEHFKESEK VSDIMLQKLK SLHLKKKTLD KEVIDCDSDE 550 560 570 580 590 600 AKSIRDVPTL LGAKLDKYHS LNEELDFLVT SYEEIIECAD QRLAISHSQI AHLEERNKHL 610 620 630 640 EDLIRKPREK ARKPRSKSLE NHPKSMTMMP ALFKENRNDL D
	Isoform 3. Checksum: 9AA090C11DEF4A08	FASTA	824	95,424
	10 20 30 40 50 60 MNKDYQKFWS SPSDPVHFEV DTSHEKVESM SESDTMNVSN LSQGVMLSHS PICMETTGTT 70 80 90 100 110 120 CDLPQNEIKN FERENEYEST LCEDAYGTLD NLLNDNNIEN YSTNALIQPV DTISISSLRQ 130 140 150 160 170 180 FETVCKFHWV EAFDDEMTEK PEFQSQVYNY AKDNNIKQDS FKEENPMETS VSANTDQLGN 190 200 210 220 230 240 EYFRQPPPRS PPLIHCSGEM LKFTEKSLAK SIAKESALNP SQPPSFLCKT AVPSKEIQNY 250 260 270 280 290 300 GEIPEMSVSY EKEVTAEGVE RPEIVSTWSS AGISWRSEAC RENCEMPDWE QSAESLQPVQ 310 320 330 340 350 360 EDMALNEVLQ KLKHTNRKQE VRIQELQCSN LYLEKRVKEL QMKITKQQVF IDVINKLKEN 370 380 390 400 410 420 VEELIEDKYK IILEKNDTKK TLQNLEEVLA NTQKHLQESR NDKEMLQLQF KKIKANYVCL 430 440 450 460 470 480 QERYMTEMQQ KNKSVSQYLE MDKTLSKKEE EVERLQQLKK ELEKATASAL DLLKREKEAQ 490 500 510 520 530 540 EQEFLSLQEE FQKLEKENLE ERQKLKSRLE KLLTQVRNLQ FMSENERTKN IKLQQQINEV 550 560 570 580 590 600 KNENAKLKQQ VARSEEQNYV PKFETAQLKD QLEEVLKSDI TKDTKTTHSN LLPDCSPCEE 610 620 630 640 650 660 RLNPADIKRA SQLASKMHSL LALMVGLLTC QDIINSDAEH FKESEKVSDI MLQKLKSLHL 670 680 690 700 710 720 KKKTLDKELL KHKDRITTFR ELIAKEKAFQ DHAIKVIDCD SDEAKSIRDV PTLLGAKLDK 730 740 750 760 770 780 YHSLNEELDF LVTSYEEIIE CADQRLAISH SQIAHLEERN KHLEDLIRKP REKARKPRSK 790 800 810 820 SLENHPKSMT MLAYNLYIFI GYRLGAVADA CNPSTLGGRG GWIT
	Isoform 4. Checksum: A2B72A74D31C8F44	FASTA	700	81,361
	10 20 30 40 50 60 MNKDYQKFWS SPSDPVHFEV DTSHEKVESM SESDTMNVSN LSQGVMLSHS PICMETTGTT 70 80 90 100 110 120 CDLPQNEIKN FERENEYEST LCEDAYGTLD NLLNDNNIEN YSTNALIQPV DTISISSLRQ 130 140 150 160 170 180 FETVCKFHWV EAFDDEMTEK PEFQSQVYNY AKDNNIKQDS FKEENPMETS VSANTDQLGN 190 200 210 220 230 240 EYFRQPPPRS PPLIHCSGEM LKFTEKSLAK SIAKESALNP SQPPSFLCKT AVPSKEIQNY 250 260 270 280 290 300 GEIPEMSVSY EKEVTAEGVE RPEIVSTWSS AGISWRSEAC RENCEMPDWE QSAESLQPVQ 310 320 330 340 350 360 EDMALNEVLQ KLKHTNRKQE VRIQELQCSN LYLEKRVKEL QMKITKQQVF IDVINKLKEN 370 380 390 400 410 420 VEELIEDKYK IILEKNDTKK TLQNLEEVLA NTQKHLQESR NDKEMLQLQF KKIKANYVCL 430 440 450 460 470 480 QERYMTEMQQ KNKSVSQYLE MDKTLSKKEE EVERLQQLKK ELEKATASAL DLLKREKEAQ 490 500 510 520 530 540 EQEFLSLQEE FQKLEKENLE ERQKLKSRLE KLLTQVRNLQ FMSENERTKN IKLQQQINEV 550 560 570 580 590 600 KNENAKLKQQ VARSEEQNYV PKFETAQLKD QLEEVLKSDI TKDTKTTHSN LLPDCSPCEE 610 620 630 640 650 660 RLNPADIKRA SQLASKMHSL LALMVGLLTC QDIINSDAEH FKESEKVSDI MLQKLKSLHL 670 680 690 700 KKKTLDKELL KHKDRITTFR ELIAKEKAFQ DHAIKVFQGV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of a novel cancer/testis antigen gene CAGE-1." Park S., Lim Y., Lee D., Cho B., Bang Y.-J., Sung S., Kim H.-Y., Kim D.-K., Lee Y.-S., Song Y., Jeoung D.I. Biochim. Biophys. Acta 1625:173-182(2003) [PubMed: 12531476] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Testis.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Testis.
[3]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed: 14574404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4). Tissue: Testis.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF414185 mRNA. Translation: AAO47239.1. AK097760 mRNA. Translation: BAC05162.1. AL139095 Genomic DNA. Translation: CAI16451.1. Sequence problems. BC026194 mRNA. Translation: AAH26194.1. BC033545 mRNA. Translation: AAH33545.1.
IPI	IPI00175214. IPI00479345. IPI00514516. IPI00830133.
RefSeq	NP_995586.1.
UniGene	Hs.715151
3D structure databases
HSSP	HSSP built from PDB template 1NKP based on UniProtKB P01106.
ModBase	Search...
PTM databases
PhosphoSite	Q8TC20.
Genome annotation databases
GeneID	285782.
KEGG	hsa:285782.
Organism-specific databases
GeneCards	GC06M007273.
HGNC	HGNC:21622. CAGE1.
MIM	608304. gene.
PharmGKB	PA134933951.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q8TC20.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	95779.
SOURCE	Search...

Entry information

Entry name

CAGE1_HUMAN

Accession

Primary (citable) accession number: Q8TC20
Secondary accession number(s): Q5TAM0, Q86TM4, Q8N7R5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	March 20, 2007
Last modified:	May 5, 2009
This is version 47 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)