Q8TBX8 (PI42C_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphatidylinositol 5-phosphate 4-kinase type-2 gamma
EC=2.7.1.149

Alternative name(s):
Phosphatidylinositol 5-phosphate 4-kinase type II gamma
Short name=PI(5)P 4-kinase type II gamma
Short name=PIP4KII-gamma

Gene names

Name:	PIP4K2C
Synonyms:	PIP5K2C

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play an important role in the production of Phosphatidylinositol bisphosphate (PIP2), in the endoplasmic reticulum By similarity.
Catalytic activity	ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.
Subcellular location	Cytoplasm. Membrane. Note: Mostly found in the cytosol and surrounding plasma membrane. However, its presence in the endoplasmic reticulum seems to be a prerequisite for PIP2 synthesis By similarity.
Sequence similarities	Contains 1 PIPK domain.
Sequence caution	The sequence BAB15223.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	phosphatidylinositol phosphorylation Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from direct assay. Source: HPA membrane Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from direct assay. Source: HPA
Molecular_function	1-phosphatidylinositol-5-phosphate 4-kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		2	EBI-1383637,EBI-1383637

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8TBX8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8TBX8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     124-171: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q8TBX8-3)

The sequence of this isoform differs from the canonical sequence as follows:
     83-100: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 421

421

Phosphatidylinositol 5-phosphate 4-kinase type-2 gamma

PRO_0000285750

Regions

Domain

43 – 420

378

PIPK

Amino acid modifications

Modified residue

26

1

Phosphoserine Ref.5

Modified residue

349

1

Phosphoserine Ref.5

Natural variations

Alternative sequence

83 – 100

18

Missing in isoform 3.

VSP_043369

Alternative sequence

124 – 171

48

Missing in isoform 2.

VSP_042929

Natural variant

84

1

V → A.
Corresponds to variant rs17550713 [ dbSNP | Ensembl ].

VAR_032049

Natural variant

241

1

K → R. Ref.4
Corresponds to variant rs17852569 [ dbSNP | Ensembl ].

VAR_032050

Natural variant

300

1

A → G.
Corresponds to variant rs2277319 [ dbSNP | Ensembl ].

VAR_032051

Secondary structure

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421

Helix Strand Turn

Details...

Helix

46 – 62

17

Helix

73 – 77

5

Beta strand

79 – 85

7

Beta strand

87 – 89

3

Beta strand

92 – 94

3

Beta strand

96 – 104

9

Helix

105 – 113

9

Turn

114 – 116

3

Helix

119 – 127

9

Beta strand

147 – 153

7

Helix

156 – 175

20

Beta strand

180 – 182

3

Beta strand

185 – 190

6

Beta strand

193 – 195

3

Beta strand

199 – 204

6

Beta strand

209 – 211

3

Helix

260 – 275

16

Helix

405 – 415

11

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 11, 2011. Version 3. Checksum: 65D1CBCF0826D476	FASTA	421	47,300
	10 20 30 40 50 60 MASSSVPPAT VSAATAGPGP GFGFASKTKK KHFVQQKVKV FRAADPLVGV FLWGVAHSIN 70 80 90 100 110 120 ELSQVPPPVM LLPDDFKASS KIKVNNHLFH RENLPSHFKF KEYCPQVFRN LRDRFGIDDQ 130 140 150 160 170 180 DYLVSLTRNP PSESEGSDGR FLISYDRTLV IKEVSSEDIA DMHSNLSNYH QYIVKCHGNT 190 200 210 220 230 240 LLPQFLGMYR VSVDNEDSYM LVMRNMFSHR LPVHRKYDLK GSLVSREASD KEKVKELPTL 250 260 270 280 290 300 KDMDFLNKNQ KVYIGEEEKK IFLEKLKRDV EFLVQLKIMD YSLLLGIHDI IRGSEPEEEA 310 320 330 340 350 360 PVREDESEVD GDCSLTGPPA LVGSYGTSPE GIGGYIHSHR PLGPGEFESF IDVYAIRSAE 370 380 390 400 410 420 GAPQKEVYFM GLIDILTQYD AKKKAAHAAK TVKHGAGAEI STVHPEQYAK RFLDFITNIF A
	Isoform 2 [UniParc]. Checksum: 45948B5F37090624	FASTA	373	41,922
	10 20 30 40 50 60 MASSSVPPAT VSAATAGPGP GFGFASKTKK KHFVQQKVKV FRAADPLVGV FLWGVAHSIN 70 80 90 100 110 120 ELSQVPPPVM LLPDDFKASS KIKVNNHLFH RENLPSHFKF KEYCPQVFRN LRDRFGIDDQ 130 140 150 160 170 180 DYLYIVKCHG NTLLPQFLGM YRVSVDNEDS YMLVMRNMFS HRLPVHRKYD LKGSLVSREA 190 200 210 220 230 240 SDKEKVKELP TLKDMDFLNK NQKVYIGEEE KKIFLEKLKR DVEFLVQLKI MDYSLLLGIH 250 260 270 280 290 300 DIIRGSEPEE EAPVREDESE VDGDCSLTGP PALVGSYGTS PEGIGGYIHS HRPLGPGEFE 310 320 330 340 350 360 SFIDVYAIRS AEGAPQKEVY FMGLIDILTQ YDAKKKAAHA AKTVKHGAGA EISTVHPEQY 370 AKRFLDFITN IFA
	Isoform 3 [UniParc]. Checksum: A3980DE66031431E	FASTA	403	45,053
	10 20 30 40 50 60 MASSSVPPAT VSAATAGPGP GFGFASKTKK KHFVQQKVKV FRAADPLVGV FLWGVAHSIN 70 80 90 100 110 120 ELSQVPPPVM LLPDDFKASS KIKEYCPQVF RNLRDRFGID DQDYLVSLTR NPPSESEGSD 130 140 150 160 170 180 GRFLISYDRT LVIKEVSSED IADMHSNLSN YHQYIVKCHG NTLLPQFLGM YRVSVDNEDS 190 200 210 220 230 240 YMLVMRNMFS HRLPVHRKYD LKGSLVSREA SDKEKVKELP TLKDMDFLNK NQKVYIGEEE 250 260 270 280 290 300 KKIFLEKLKR DVEFLVQLKI MDYSLLLGIH DIIRGSEPEE EAPVREDESE VDGDCSLTGP 310 320 330 340 350 360 PALVGSYGTS PEGIGGYIHS HRPLGPGEFE SFIDVYAIRS AEGAPQKEVY FMGLIDILTQ 370 380 390 400 YDAKKKAAHA AKTVKHGAGA EISTVHPEQY AKRFLDFITN IFA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3). Tissue: Placenta and Testis.
[2]	"The finished DNA sequence of human chromosome 12." Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R. , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A. Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ARG-241. Tissue: Testis.
[5]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26 AND SER-349, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[6]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]	"Structure of human phosphatidylinositol-4-phosphate 5-kinase, type II, gamma." Structural genomics consortium (SGC) Submitted (APR-2007) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 32-421.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AK025708 mRNA. Translation: BAB15223.1. Different initiation.
AK297243 mRNA. Translation: BAG59723.1.
AK302254 mRNA. Translation: BAG63606.1.
AK315588 mRNA. Translation: BAG37960.1.
AC022506 Genomic DNA. No translation available.
CH471054 Genomic DNA. Translation: EAW97031.1.
BC028596 mRNA. Translation: AAH28596.1.

IPI

IPI00152303.
IPI00929626.
IPI00929676.

RefSeq

NP_001139730.1. NM_001146258.1.
NP_001139731.1. NM_001146259.1.
NP_001139732.1. NM_001146260.1.
NP_079055.3. NM_024779.4.

UniGene

Hs.144502.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2GK9	X-ray	2.80	A/B/C/D	32-421	[»]

ProteinModelPortal

Q8TBX8.

SMR

Q8TBX8. Positions 39-420.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q8TBX8. 1 interaction.

STRING

9606.ENSP00000347032.

PTM databases

PhosphoSite

Q8TBX8.

Polymorphism databases

DMDM

146325055.

Proteomic databases

PaxDb

Q8TBX8.

PeptideAtlas

Q8TBX8.

PRIDE

Q8TBX8.

Protocols and materials databases

DNASU

79837.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000354947; ENSP00000347032; ENSG00000166908.
ENST00000422156; ENSP00000412035; ENSG00000166908.
ENST00000540759; ENSP00000439878; ENSG00000166908.
ENST00000550465; ENSP00000447390; ENSG00000166908.

GeneID

79837.

KEGG

hsa:79837.

UCSC

uc001sot.3. human.

Organism-specific databases

CTD

79837.

GeneCards

GC12P057985.

H-InvDB

HIX0201836.

HGNC

HGNC:23786. PIP4K2C.

HPA

HPA028658.

neXtProt

NX_Q8TBX8.

PharmGKB

PA162399665.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG5253.

HOGENOM

HOG000007832.

HOVERGEN

HBG000072.

InParanoid

Q8TBX8.

KO

K00920.

OMA

EGEGDCG.

OrthoDB

EOG4TMR2C.

PhylomeDB

Q8TBX8.

Gene expression databases

ArrayExpress

Q8TBX8.

Bgee

Q8TBX8.

CleanEx

HS_PIP4K2C.

Genevestigator

Q8TBX8.

Family and domain databases

InterPro

IPR023610. PInositol-4-P-5-kinase.
IPR002498. PInositol-4-P-5-kinase_core.
IPR016034. PInositol-4P-5-kinase_core_sub.
[Graphical view]

PANTHER

PTHR23086. PTHR23086. 1 hit.

Pfam

PF01504. PIP5K. 1 hit.
[Graphical view]

SMART

SM00330. PIPKc. 1 hit.
[Graphical view]

PROSITE

PS51455. PIPK. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

Q8TBX8.

ChEMBL

CHEMBL1770034.

ChiTaRS

PIP4K2C. human.

EvolutionaryTrace

Q8TBX8.

GenomeRNAi

79837.

NextBio

69498.

Entry information

Entry name

PI42C_HUMAN

Accession

Primary (citable) accession number: Q8TBX8
Secondary accession number(s): B2RDL3 , B4DM11, B4DY44, Q9H6N2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	January 11, 2011
Last modified:	May 1, 2013
This is version 87 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.