Reviewed, UniProtKB/Swiss-Prot Q8TBM8 (DJB14_HUMAN)

Names and origin

Protein names

Recommended name:
DnaJ homolog subfamily B member 14

Gene names

Name:	DNAJB14
ORF Names:	UNQ9427/PRO34683

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May act as a co-chaperone By similarity.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Sequence similarities	Contains 1 J domain.
Sequence caution	The sequence AAQ88639.1 differs from that shown. Reason: Erroneous termination at position 355. Translated as Arg. The sequence BAB14893.1 differs from that shown. Reason: Erroneous termination at position 190. Translated as Arg. The sequence CAD97796.1 differs from that shown. Reason: Miscellaneous discrepancy. Intron retention.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane
Molecular function	Chaperone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein folding Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	heat shock protein binding Inferred from electronic annotation. Source: InterPro unfolded protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8TBM8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8TBM8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-85: Missing.
     86-102: GEGGKGYTKDQVDGVLS → MDIGTLIWDGGPVPNTH

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 379	379	DnaJ homolog subfamily B member 14		PRO_0000281478
Regions
	Transmembrane	245 – 265	21	Potential
	Domain	108 – 172	65	J
Natural variations
	Alternative sequence	1 – 85	85	Missing in isoform 2.		VSP_024006
	Alternative sequence	86 – 102	17	GEGGK…DGVLS → MDIGTLIWDGGPVPNTH in isoform 2.		VSP_024007
Experimental info
	Sequence conflict	143	1	P → L in BAB14893. Ref.6
	Sequence conflict	187	1	N → T in AAO31693. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 02FB6D940DECA170	FASTA	379	42,516
	10 20 30 40 50 60 MEGNRDEAEK CVEIAREALN AGNREKAQRF LQKAEKLYPL PSARALLEII MKNGSTAGNS 70 80 90 100 110 120 PHCRKPSGSG DQSKPNCTKD STSGSGEGGK GYTKDQVDGV LSINKCKNYY EVLGVTKDAG 130 140 150 160 170 180 DEDLKKAYRK LALKFHPDKN HAPGATDAFK KIGNAYAVLS NPEKRKQYDL TGNEEQACNH 190 200 210 220 230 240 QNNGRFNFHR GCEADITPED LFNIFFGGGF PSGSVHSFSN GRAGYSQQHQ HRHSGHEREE 250 260 270 280 290 300 ERGDGGFSVF IQLMPIIVLI LVSLLSQLMV SNPPYSLYPR SGTGQTIKMQ TENLGVVYYV 310 320 330 340 350 360 NKDFKNEYKG MLLQKVEKSV EEDYVTNIRN NCWKERQQKT DMQYAAKVYR DDRLRRKADA 370 LSMDNCKELE RLTSLYKGG
	Isoform 2. Checksum: 9D28BE180150576E	FASTA	294	33,471
	10 20 30 40 50 60 MDIGTLIWDG GPVPNTHINK CKNYYEVLGV TKDAGDEDLK KAYRKLALKF HPDKNHAPGA 70 80 90 100 110 120 TDAFKKIGNA YAVLSNPEKR KQYDLTGNEE QACNHQNNGR FNFHRGCEAD ITPEDLFNIF 130 140 150 160 170 180 FGGGFPSGSV HSFSNGRAGY SQQHQHRHSG HEREEERGDG GFSVFIQLMP IIVLILVSLL 190 200 210 220 230 240 SQLMVSNPPY SLYPRSGTGQ TIKMQTENLG VVYYVNKDFK NEYKGMLLQK VEKSVEEDYV 250 260 270 280 290 TNIRNNCWKE RQQKTDMQYA AKVYRDDRLR RKADALSMDN CKELERLTSL YKGG

References

[1]	Sha J.H., Zhou Z.M., Xu M. Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Testis.
[2]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-101 (ISOFORM 1). Tissue: Fetal kidney and Spinal cord.
[4]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Testis.
[6]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 107-379. Tissue: Placenta.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY186740 mRNA. Translation: AAO31693.1. AY358272 mRNA. Translation: AAQ88639.1. AL833076 mRNA. Translation: CAD89928.1. BX537609 mRNA. Translation: CAD97796.1. Sequence problems. AC097460 Genomic DNA. Translation: AAY41012.1. BC022248 mRNA. Translation: AAH22248.1. AK024343 mRNA. Translation: BAB14893.1. Sequence problems. AK023700 mRNA. Translation: BAB14645.1. Different initiation.
IPI	IPI00178504. IPI00410274.
RefSeq	NP_001026893.1.
UniGene	Hs.577426
3D structure databases
HSSP	HSSP built from PDB template 2CTP based on UniProtKB Q9NXW2.
SMR	Q8TBM8. Positions 82-167, 105-212.
ModBase	Search...
Proteomic databases
PRIDE	Q8TBM8.
Genome annotation databases
Ensembl	ENST00000442697; ENSP00000404381; ENSG00000164031; Homo sapiens. [Genome view]
GeneID	79982.
KEGG	hsa:79982.
UCSC	uc003hvj.1. human. uc003hvl.1. human.
Organism-specific databases
CTD	79982.
GeneCards	GC04M101039.
H-InvDB	HIX0004403.
HGNC	HGNC:25881. DNAJB14.
PharmGKB	PA142671973.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG15962.
HOGENOM	HBG737361.
HOVERGEN	HBG051373.
InParanoid	Q8TBM8.
OMA	GFSMFIQ.
PhylomeDB	Q8TBM8.
Gene expression databases
ArrayExpress	Q8TBM8.
Bgee	Q8TBM8.
CleanEx	HS_DNAJB14.
Genevestigator	Q8TBM8.
Family and domain databases
InterPro	IPR001623. DnaJ_N. IPR015399. DUF1977_DnaJ-like. IPR018253. Heat_shock_DnaJ_CS. IPR015609. Hsp40/DnaJ_Rel. IPR003095. Hsp_DnaJ. [Graphical view]
Gene3D	G3DSA:1.10.287.110. DnaJ_N. 1 hit.
PANTHER	PTHR11821. Hsp40/DnaJ_Rel. 1 hit.
Pfam	PF00226. DnaJ. 1 hit. PF09320. DUF1977. 1 hit. [Graphical view]
PRINTS	PR00625. DNAJPROTEIN.
SMART	SM00271. DnaJ. 1 hit. [Graphical view]
SUPFAM	SSF46565. DnaJ_N. 1 hit.
PROSITE	PS00636. DNAJ_1. 1 hit. PS50076. DNAJ_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	70021.

Entry information

Entry name

DJB14_HUMAN

Accession

Primary (citable) accession number: Q8TBM8
Secondary accession number(s): Q6UXN1 , Q7Z3P0, Q86TA7, Q86TM0, Q9GZU9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	June 1, 2002
Last modified:	March 2, 2010
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.