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Protein

DnaJ homolog subfamily B member 14

Gene

DNAJB14

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a co-chaperone with HSPA8 and promotes the degradation of misfolded transmembrane proteins in the ER-associated degradation (ERAD) pathway.1 Publication

Keywords - Molecular functioni

Chaperone

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog subfamily B member 14
Gene namesi
Name:DNAJB14
ORF Names:UNQ9427/PRO34683
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:25881. DNAJB14.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 244CytoplasmicSequence analysisAdd BLAST244
Transmembranei245 – 265HelicalSequence analysisAdd BLAST21
Topological domaini266 – 379LumenalSequence analysisAdd BLAST114

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000164031.
PharmGKBiPA142671973.

Polymorphism and mutation databases

DMDMi74751385.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002814781 – 379DnaJ homolog subfamily B member 14Add BLAST379

Proteomic databases

EPDiQ8TBM8.
MaxQBiQ8TBM8.
PaxDbiQ8TBM8.
PeptideAtlasiQ8TBM8.
PRIDEiQ8TBM8.

PTM databases

iPTMnetiQ8TBM8.
PhosphoSitePlusiQ8TBM8.

Expressioni

Gene expression databases

BgeeiENSG00000164031.
CleanExiHS_DNAJB14.
ExpressionAtlasiQ8TBM8. baseline and differential.
GenevisibleiQ8TBM8. HS.

Organism-specific databases

HPAiHPA036016.

Interactioni

Subunit structurei

Interacts (via J domain) with HSPA8/Hsc70.1 Publication

Protein-protein interaction databases

BioGridi123047. 6 interactors.
IntActiQ8TBM8. 3 interactors.
STRINGi9606.ENSP00000404381.

Structurei

3D structure databases

ProteinModelPortaliQ8TBM8.
SMRiQ8TBM8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini108 – 172JPROSITE-ProRule annotationAdd BLAST65

Sequence similaritiesi

Contains 1 J domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0714. Eukaryota.
COG0484. LUCA.
GeneTreeiENSGT00840000129767.
HOGENOMiHOG000233608.
HOVERGENiHBG051373.
InParanoidiQ8TBM8.
KOiK09520.
OMAiKKAELMT.
OrthoDBiEOG091G0CE9.
PhylomeDBiQ8TBM8.
TreeFamiTF105145.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
InterProiIPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR015399. DUF1977_DnaJ-like.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF09320. DUF1977. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8TBM8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEGNRDEAEK CVEIAREALN AGNREKAQRF LQKAEKLYPL PSARALLEII
60 70 80 90 100
MKNGSTAGNS PHCRKPSGSG DQSKPNCTKD STSGSGEGGK GYTKDQVDGV
110 120 130 140 150
LSINKCKNYY EVLGVTKDAG DEDLKKAYRK LALKFHPDKN HAPGATDAFK
160 170 180 190 200
KIGNAYAVLS NPEKRKQYDL TGNEEQACNH QNNGRFNFHR GCEADITPED
210 220 230 240 250
LFNIFFGGGF PSGSVHSFSN GRAGYSQQHQ HRHSGHEREE ERGDGGFSVF
260 270 280 290 300
IQLMPIIVLI LVSLLSQLMV SNPPYSLYPR SGTGQTIKMQ TENLGVVYYV
310 320 330 340 350
NKDFKNEYKG MLLQKVEKSV EEDYVTNIRN NCWKERQQKT DMQYAAKVYR
360 370
DDRLRRKADA LSMDNCKELE RLTSLYKGG
Length:379
Mass (Da):42,516
Last modified:June 1, 2002 - v1
Checksum:i02FB6D940DECA170
GO
Isoform 2 (identifier: Q8TBM8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-85: Missing.
     86-102: GEGGKGYTKDQVDGVLS → MDIGTLIWDGGPVPNTH

Show »
Length:294
Mass (Da):33,471
Checksum:i9D28BE180150576E
GO

Sequence cautioni

The sequence AAQ88639 differs from that shown. Reason: Erroneous termination at position 355. Translated as Arg.Curated
The sequence BAB14645 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB14893 differs from that shown. Reason: Erroneous termination at position 190. Translated as Arg.Curated
The sequence CAD97796 differs from that shown. Intron retention.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti143P → L in BAB14893 (PubMed:14702039).Curated1
Sequence conflicti187N → T in AAO31693 (Ref. 1) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0240061 – 85Missing in isoform 2. 2 PublicationsAdd BLAST85
Alternative sequenceiVSP_02400786 – 102GEGGK…DGVLS → MDIGTLIWDGGPVPNTH in isoform 2. 2 PublicationsAdd BLAST17

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY186740 mRNA. Translation: AAO31693.1.
AY358272 mRNA. Translation: AAQ88639.1. Sequence problems.
AL833076 mRNA. Translation: CAD89928.1.
BX537609 mRNA. Translation: CAD97796.1. Sequence problems.
AC097460 Genomic DNA. Translation: AAY41012.1.
BC022248 mRNA. Translation: AAH22248.1.
AK024343 mRNA. Translation: BAB14893.1. Sequence problems.
AK023700 mRNA. Translation: BAB14645.1. Different initiation.
CCDSiCCDS34035.1. [Q8TBM8-1]
RefSeqiNP_001026893.1. NM_001031723.3. [Q8TBM8-1]
NP_001265239.1. NM_001278310.1.
XP_011530564.1. XM_011532262.2. [Q8TBM8-2]
XP_011530565.1. XM_011532263.2. [Q8TBM8-2]
UniGeneiHs.577426.
Hs.643686.
Hs.713654.

Genome annotation databases

EnsembliENST00000442697; ENSP00000404381; ENSG00000164031. [Q8TBM8-1]
GeneIDi79982.
KEGGihsa:79982.
UCSCiuc003hvl.5. human. [Q8TBM8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY186740 mRNA. Translation: AAO31693.1.
AY358272 mRNA. Translation: AAQ88639.1. Sequence problems.
AL833076 mRNA. Translation: CAD89928.1.
BX537609 mRNA. Translation: CAD97796.1. Sequence problems.
AC097460 Genomic DNA. Translation: AAY41012.1.
BC022248 mRNA. Translation: AAH22248.1.
AK024343 mRNA. Translation: BAB14893.1. Sequence problems.
AK023700 mRNA. Translation: BAB14645.1. Different initiation.
CCDSiCCDS34035.1. [Q8TBM8-1]
RefSeqiNP_001026893.1. NM_001031723.3. [Q8TBM8-1]
NP_001265239.1. NM_001278310.1.
XP_011530564.1. XM_011532262.2. [Q8TBM8-2]
XP_011530565.1. XM_011532263.2. [Q8TBM8-2]
UniGeneiHs.577426.
Hs.643686.
Hs.713654.

3D structure databases

ProteinModelPortaliQ8TBM8.
SMRiQ8TBM8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123047. 6 interactors.
IntActiQ8TBM8. 3 interactors.
STRINGi9606.ENSP00000404381.

PTM databases

iPTMnetiQ8TBM8.
PhosphoSitePlusiQ8TBM8.

Polymorphism and mutation databases

DMDMi74751385.

Proteomic databases

EPDiQ8TBM8.
MaxQBiQ8TBM8.
PaxDbiQ8TBM8.
PeptideAtlasiQ8TBM8.
PRIDEiQ8TBM8.

Protocols and materials databases

DNASUi79982.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000442697; ENSP00000404381; ENSG00000164031. [Q8TBM8-1]
GeneIDi79982.
KEGGihsa:79982.
UCSCiuc003hvl.5. human. [Q8TBM8-1]

Organism-specific databases

CTDi79982.
GeneCardsiDNAJB14.
H-InvDBHIX0059463.
HGNCiHGNC:25881. DNAJB14.
HPAiHPA036016.
neXtProtiNX_Q8TBM8.
OpenTargetsiENSG00000164031.
PharmGKBiPA142671973.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0714. Eukaryota.
COG0484. LUCA.
GeneTreeiENSGT00840000129767.
HOGENOMiHOG000233608.
HOVERGENiHBG051373.
InParanoidiQ8TBM8.
KOiK09520.
OMAiKKAELMT.
OrthoDBiEOG091G0CE9.
PhylomeDBiQ8TBM8.
TreeFamiTF105145.

Miscellaneous databases

GenomeRNAii79982.
PROiQ8TBM8.

Gene expression databases

BgeeiENSG00000164031.
CleanExiHS_DNAJB14.
ExpressionAtlasiQ8TBM8. baseline and differential.
GenevisibleiQ8TBM8. HS.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
InterProiIPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR015399. DUF1977_DnaJ-like.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF09320. DUF1977. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDJB14_HUMAN
AccessioniPrimary (citable) accession number: Q8TBM8
Secondary accession number(s): Q6UXN1
, Q7Z3P0, Q86TA7, Q86TM0, Q9GZU9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: June 1, 2002
Last modified: November 30, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.