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Protein

DnaJ homolog subfamily B member 14

Gene

DNAJB14

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a co-chaperone with HSPA8/Hsc70; required to promote protein folding and trafficking, prevent aggregation of client proteins, and promote unfolded proteins to endoplasmic reticulum-associated degradation (ERAD) pathway (PubMed:24732912). Acts by determining HSPA8/Hsc70's ATPase and polypeptide-binding activities (PubMed:24732912). Can also act independently of HSPA8/Hsc70: together with DNAJB12, acts as a chaperone that promotes maturation of potassium channels KCND2 and KCNH2 by stabilizing nascent channel subunits and assembling them into tetramers (PubMed:27916661). While stabilization of nascent channel proteins is dependent on HSPA8/Hsc70, the process of oligomerization of channel subunits is independent of HSPA8/Hsc70 (PubMed:27916661). When overexpressed, forms membranous structures together with DNAJB12 and HSPA8/Hsc70 within the nucleus; the role of these structures, named DJANGOs, is still unclear (PubMed:24732912).3 Publications
(Microbial infection) In case of infection by polyomavirus, involved in the virus endoplasmic reticulum membrane penetration and infection (PubMed:21673190, PubMed:24675744).2 Publications

GO - Molecular functioni

  • Hsp70 protein binding Source: UniProtKB

GO - Biological processi

  • cellular protein complex assembly Source: UniProtKB
  • chaperone cofactor-dependent protein refolding Source: UniProtKB
  • chaperone mediated protein folding requiring cofactor Source: UniProtKB

Keywordsi

Molecular functionChaperone

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog subfamily B member 14Imported
Gene namesi
Name:DNAJB14Imported
ORF Names:UNQ9427/PRO346831 Publication
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiHostDB:ENSG00000164031.16.
HGNCiHGNC:25881. DNAJB14.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 244CytoplasmicSequence analysisAdd BLAST244
Transmembranei245 – 265HelicalSequence analysisAdd BLAST21
Topological domaini266 – 379LumenalSequence analysisAdd BLAST114

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi136H → Q: Abolishes interaction with HSPA8/Hsc70. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000164031.
PharmGKBiPA142671973.

Polymorphism and mutation databases

DMDMi74751385.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002814781 – 379DnaJ homolog subfamily B member 14Add BLAST379

Proteomic databases

EPDiQ8TBM8.
MaxQBiQ8TBM8.
PaxDbiQ8TBM8.
PeptideAtlasiQ8TBM8.
PRIDEiQ8TBM8.

PTM databases

iPTMnetiQ8TBM8.
PhosphoSitePlusiQ8TBM8.

Expressioni

Gene expression databases

BgeeiENSG00000164031.
CleanExiHS_DNAJB14.
ExpressionAtlasiQ8TBM8. baseline and differential.
GenevisibleiQ8TBM8. HS.

Organism-specific databases

HPAiHPA036016.

Interactioni

Subunit structurei

Interacts (via J domain) with HSPA8/Hsc70 (PubMed:23018488, PubMed:24732912, PubMed:27916661). Forms a multiprotein complex, at least composed of DNAJB12, DNAJB14, HSPA8/Hsc70 and SGTA; interaction with DNAJB14 and HSPA8/Hsc70 is direct (PubMed:24675744).4 Publications

GO - Molecular functioni

  • Hsp70 protein binding Source: UniProtKB

Protein-protein interaction databases

BioGridi123047. 6 interactors.
IntActiQ8TBM8. 3 interactors.
STRINGi9606.ENSP00000404381.

Structurei

3D structure databases

ProteinModelPortaliQ8TBM8.
SMRiQ8TBM8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini108 – 172JPROSITE-ProRule annotationAdd BLAST65

Sequence similaritiesi

Belongs to the DnaJ family. DNAJB12/DNAJB14 subfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0714. Eukaryota.
COG0484. LUCA.
GeneTreeiENSGT00840000129767.
HOGENOMiHOG000233608.
HOVERGENiHBG051373.
InParanoidiQ8TBM8.
KOiK09520.
OMAiMENCKEL.
OrthoDBiEOG091G0CE9.
PhylomeDBiQ8TBM8.
TreeFamiTF105145.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
InterProiView protein in InterPro
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR015399. DUF1977_DnaJ-like.
PfamiView protein in Pfam
PF00226. DnaJ. 1 hit.
PF09320. DUF1977. 1 hit.
PRINTSiPR00625. JDOMAIN.
SMARTiView protein in SMART
SM00271. DnaJ. 1 hit.
SUPFAMiSSF46565. SSF46565. 1 hit.
PROSITEiView protein in PROSITE
PS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8TBM8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEGNRDEAEK CVEIAREALN AGNREKAQRF LQKAEKLYPL PSARALLEII
60 70 80 90 100
MKNGSTAGNS PHCRKPSGSG DQSKPNCTKD STSGSGEGGK GYTKDQVDGV
110 120 130 140 150
LSINKCKNYY EVLGVTKDAG DEDLKKAYRK LALKFHPDKN HAPGATDAFK
160 170 180 190 200
KIGNAYAVLS NPEKRKQYDL TGNEEQACNH QNNGRFNFHR GCEADITPED
210 220 230 240 250
LFNIFFGGGF PSGSVHSFSN GRAGYSQQHQ HRHSGHEREE ERGDGGFSVF
260 270 280 290 300
IQLMPIIVLI LVSLLSQLMV SNPPYSLYPR SGTGQTIKMQ TENLGVVYYV
310 320 330 340 350
NKDFKNEYKG MLLQKVEKSV EEDYVTNIRN NCWKERQQKT DMQYAAKVYR
360 370
DDRLRRKADA LSMDNCKELE RLTSLYKGG
Length:379
Mass (Da):42,516
Last modified:June 1, 2002 - v1
Checksum:i02FB6D940DECA170
GO
Isoform 2 (identifier: Q8TBM8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-85: Missing.
     86-102: GEGGKGYTKDQVDGVLS → MDIGTLIWDGGPVPNTH

Show »
Length:294
Mass (Da):33,471
Checksum:i9D28BE180150576E
GO

Sequence cautioni

The sequence AAQ88639 differs from that shown. Reason: Erroneous termination at position 355. Translated as Arg.Curated
The sequence BAB14645 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB14893 differs from that shown. Reason: Erroneous termination at position 190. Translated as Arg.Curated
The sequence CAD97796 differs from that shown. Intron retention.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti143P → L in BAB14893 (PubMed:14702039).Curated1
Sequence conflicti187N → T in AAO31693 (Ref. 1) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0240061 – 85Missing in isoform 2. 2 PublicationsAdd BLAST85
Alternative sequenceiVSP_02400786 – 102GEGGK…DGVLS → MDIGTLIWDGGPVPNTH in isoform 2. 2 PublicationsAdd BLAST17

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY186740 mRNA. Translation: AAO31693.1.
AY358272 mRNA. Translation: AAQ88639.1. Sequence problems.
AL833076 mRNA. Translation: CAD89928.1.
BX537609 mRNA. Translation: CAD97796.1. Sequence problems.
AC097460 Genomic DNA. Translation: AAY41012.1.
BC022248 mRNA. Translation: AAH22248.1.
AK024343 mRNA. Translation: BAB14893.1. Sequence problems.
AK023700 mRNA. Translation: BAB14645.1. Different initiation.
CCDSiCCDS34035.1. [Q8TBM8-1]
RefSeqiNP_001026893.1. NM_001031723.3. [Q8TBM8-1]
NP_001265239.1. NM_001278310.1.
XP_011530564.1. XM_011532262.2. [Q8TBM8-2]
XP_011530565.1. XM_011532263.2. [Q8TBM8-2]
UniGeneiHs.577426.
Hs.643686.
Hs.713654.

Genome annotation databases

EnsembliENST00000442697; ENSP00000404381; ENSG00000164031. [Q8TBM8-1]
GeneIDi79982.
KEGGihsa:79982.
UCSCiuc003hvl.5. human. [Q8TBM8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiDJB14_HUMAN
AccessioniPrimary (citable) accession number: Q8TBM8
Secondary accession number(s): Q6UXN1
, Q7Z3P0, Q86TA7, Q86TM0, Q9GZU9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: June 1, 2002
Last modified: September 27, 2017
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families