Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

N-lysine methyltransferase SETD6

Gene

SETD6

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein-lysine N-methyltransferase. Monomethylates 'Lys-310' of the RELA subunit of NF-kappa-B complex, leading to down-regulate NF-kappa-B transcription factor activity (PubMed:21131967). Monomethylates 'Lys-8' of H2AZ (H2AZK8me1) (PubMed:23324626). Required for the maintenance of embryonic stem cell self-renewal (By similarity).By similarity2 Publications

GO - Molecular functioni

  • NF-kappaB binding Source: UniProtKB
  • protein-lysine N-methyltransferase activity Source: UniProtKB

GO - Biological processi

  • histone lysine methylation Source: UniProtKB
  • negative regulation of NF-kappaB transcription factor activity Source: UniProtKB
  • peptidyl-lysine monomethylation Source: UniProtKB
  • regulation of inflammatory response Source: UniProtKB
  • stem cell differentiation Source: UniProtKB
  • stem cell population maintenance Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciZFISH:ENSG00000103037-MONOMER.
ReactomeiR-HSA-3214841. PKMTs methylate histone lysines.

Names & Taxonomyi

Protein namesi
Recommended name:
N-lysine methyltransferase SETD6 (EC:2.1.1.-)
Alternative name(s):
SET domain-containing protein 6
Gene namesi
Name:SETD6
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:26116. SETD6.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: HPA
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi285Y → A: Abolishes methyltransferase activity. 1 Publication1

Organism-specific databases

DisGeNETi79918.
OpenTargetsiENSG00000103037.
PharmGKBiPA143485614.

Polymorphism and mutation databases

BioMutaiSETD6.
DMDMi308153495.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002818891 – 473N-lysine methyltransferase SETD6Add BLAST473

Proteomic databases

EPDiQ8TBK2.
MaxQBiQ8TBK2.
PaxDbiQ8TBK2.
PeptideAtlasiQ8TBK2.
PRIDEiQ8TBK2.

PTM databases

iPTMnetiQ8TBK2.
PhosphoSitePlusiQ8TBK2.

Expressioni

Gene expression databases

BgeeiENSG00000103037.
CleanExiHS_SETD6.
ExpressionAtlasiQ8TBK2. baseline and differential.
GenevisibleiQ8TBK2. HS.

Organism-specific databases

HPAiHPA041481.
HPA053546.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
RelaQ042074EBI-3863032,EBI-644400From a different organism.

GO - Molecular functioni

  • NF-kappaB binding Source: UniProtKB

Protein-protein interaction databases

BioGridi122996. 1 interactor.
IntActiQ8TBK2. 3 interactors.
STRINGi9606.ENSP00000219315.

Structurei

Secondary structure

1473
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi20 – 32Combined sources13
Beta strandi65 – 70Combined sources6
Beta strandi72 – 81Combined sources10
Beta strandi88 – 93Combined sources6
Helixi94 – 96Combined sources3
Beta strandi97 – 99Combined sources3
Turni100 – 102Combined sources3
Helixi106 – 111Combined sources6
Helixi114 – 116Combined sources3
Beta strandi119 – 121Combined sources3
Helixi123 – 134Combined sources12
Helixi141 – 144Combined sources4
Helixi150 – 152Combined sources3
Helixi156 – 158Combined sources3
Helixi161 – 168Combined sources8
Helixi173 – 190Combined sources18
Helixi192 – 198Combined sources7
Turni200 – 202Combined sources3
Helixi205 – 207Combined sources3
Helixi210 – 223Combined sources14
Helixi247 – 249Combined sources3
Beta strandi257 – 262Combined sources6
Beta strandi264 – 273Combined sources10
Beta strandi280 – 283Combined sources4
Helixi290 – 297Combined sources8
Beta strandi310 – 314Combined sources5
Helixi315 – 324Combined sources10
Helixi329 – 344Combined sources16
Beta strandi353 – 363Combined sources11
Helixi364 – 375Combined sources12
Helixi378 – 386Combined sources9
Turni402 – 404Combined sources3
Helixi405 – 407Combined sources3
Helixi410 – 424Combined sources15
Beta strandi427 – 429Combined sources3
Helixi431 – 439Combined sources9
Helixi441 – 446Combined sources6
Helixi449 – 471Combined sources23

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3QXYX-ray2.09A/B1-473[»]
3RC0X-ray2.19A/B1-473[»]
ProteinModelPortaliQ8TBK2.
SMRiQ8TBK2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini60 – 286SETPROSITE-ProRule annotationAdd BLAST227

Sequence similaritiesi

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. SETD6 subfamily.PROSITE-ProRule annotation
Contains 1 SET domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1338. Eukaryota.
ENOG410ZN0H. LUCA.
GeneTreeiENSGT00850000132334.
HOGENOMiHOG000264234.
HOVERGENiHBG108475.
InParanoidiQ8TBK2.
KOiK05302.
OMAiDEFITWL.
OrthoDBiEOG091G068E.
PhylomeDBiQ8TBK2.
TreeFamiTF106399.

Family and domain databases

Gene3Di3.90.1420.10. 1 hit.
InterProiIPR011383. N-lys_methylase_SETD6.
IPR015353. Rubisco_LSMT_subst-bd.
IPR001214. SET_dom.
[Graphical view]
PfamiPF09273. Rubis-subs-bind. 1 hit.
PF00856. SET. 1 hit.
[Graphical view]
PIRSFiPIRSF011771. RMS1_SET. 1 hit.
SUPFAMiSSF81822. SSF81822. 1 hit.
PROSITEiPS50280. SET. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8TBK2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATQAKRPRV AGPVDGGDLD PVACFLSWCR RVGLELSPKV SERAGGRRTR
60 70 80 90 100
GGARAALTSP PAQVAVSRQG TVAGYGMVAR ESVQAGELLF VVPRAALLSQ
110 120 130 140 150
HTCSIGGLLE RERVALQSQS GWVPLLLALL HELQAPASRW RPYFALWPEL
160 170 180 190 200
GRLEHPMFWP EEERRCLLQG TGVPEAVEKD LANIRSEYQS IVLPFMEAHP
210 220 230 240 250
DLFSLRVRSL ELYHQLVALV MAYSFQEPLE EEEDEKEPNS PVMVPAADIL
260 270 280 290 300
NHLANHNANL EYSANCLRMV ATQPIPKGHE IFNTYGQMAN WQLIHMYGFV
310 320 330 340 350
EPYPDNTDDT ADIQMVTVRE AALQGTKTEA ERHLVYERWD FLCKLEMVGE
360 370 380 390 400
EGAFVIGREE VLTEEELTTT LKVLCMPAEE FRELKDQDGG GDDKREEGSL
410 420 430 440 450
TITNIPKLKA SWRQLLQNSV LLTLQTYATD LKTDQGLLSN KEVYAKLSWR
460 470
EQQALQVRYG QKMILHQLLE LTS
Length:473
Mass (Da):53,189
Last modified:October 5, 2010 - v2
Checksum:i3BFC08F0FACEAACC
GO
Isoform 2 (identifier: Q8TBK2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     40-63: Missing.

Note: No experimental confirmation available.
Show »
Length:449
Mass (Da):50,784
Checksum:iB619F7F1CAEBB8DF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti102T → A in BAF83184 (PubMed:14702039).Curated1
Sequence conflicti118S → G in BAB15011 (PubMed:14702039).Curated1
Sequence conflicti227E → V in BAB15011 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_064590185R → S.1 PublicationCorresponds to variant rs17852020dbSNPEnsembl.1
Natural variantiVAR_064591206R → G.1 PublicationCorresponds to variant rs17852021dbSNPEnsembl.1
Natural variantiVAR_064592340D → N.Corresponds to variant rs11865588dbSNPEnsembl.1
Natural variantiVAR_064593426T → A.Corresponds to variant rs34965375dbSNPEnsembl.1
Natural variantiVAR_064594445A → V.Corresponds to variant rs36085499dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_02409340 – 63Missing in isoform 2. 1 PublicationAdd BLAST24

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK024801 mRNA. Translation: BAB15011.1.
AK290495 mRNA. Translation: BAF83184.1.
AC009118 Genomic DNA. No translation available.
BC022451 mRNA. Translation: AAH22451.1.
CCDSiCCDS10798.1. [Q8TBK2-2]
CCDS54013.1. [Q8TBK2-1]
RefSeqiNP_001153777.1. NM_001160305.2. [Q8TBK2-1]
NP_079136.2. NM_024860.3. [Q8TBK2-2]
UniGeneiHs.731691.

Genome annotation databases

EnsembliENST00000219315; ENSP00000219315; ENSG00000103037. [Q8TBK2-1]
ENST00000310682; ENSP00000310082; ENSG00000103037. [Q8TBK2-2]
GeneIDi79918.
KEGGihsa:79918.
UCSCiuc002enr.4. human. [Q8TBK2-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK024801 mRNA. Translation: BAB15011.1.
AK290495 mRNA. Translation: BAF83184.1.
AC009118 Genomic DNA. No translation available.
BC022451 mRNA. Translation: AAH22451.1.
CCDSiCCDS10798.1. [Q8TBK2-2]
CCDS54013.1. [Q8TBK2-1]
RefSeqiNP_001153777.1. NM_001160305.2. [Q8TBK2-1]
NP_079136.2. NM_024860.3. [Q8TBK2-2]
UniGeneiHs.731691.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3QXYX-ray2.09A/B1-473[»]
3RC0X-ray2.19A/B1-473[»]
ProteinModelPortaliQ8TBK2.
SMRiQ8TBK2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122996. 1 interactor.
IntActiQ8TBK2. 3 interactors.
STRINGi9606.ENSP00000219315.

PTM databases

iPTMnetiQ8TBK2.
PhosphoSitePlusiQ8TBK2.

Polymorphism and mutation databases

BioMutaiSETD6.
DMDMi308153495.

Proteomic databases

EPDiQ8TBK2.
MaxQBiQ8TBK2.
PaxDbiQ8TBK2.
PeptideAtlasiQ8TBK2.
PRIDEiQ8TBK2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000219315; ENSP00000219315; ENSG00000103037. [Q8TBK2-1]
ENST00000310682; ENSP00000310082; ENSG00000103037. [Q8TBK2-2]
GeneIDi79918.
KEGGihsa:79918.
UCSCiuc002enr.4. human. [Q8TBK2-1]

Organism-specific databases

CTDi79918.
DisGeNETi79918.
GeneCardsiSETD6.
H-InvDBHIX0013092.
HGNCiHGNC:26116. SETD6.
HPAiHPA041481.
HPA053546.
MIMi616424. gene.
neXtProtiNX_Q8TBK2.
OpenTargetsiENSG00000103037.
PharmGKBiPA143485614.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1338. Eukaryota.
ENOG410ZN0H. LUCA.
GeneTreeiENSGT00850000132334.
HOGENOMiHOG000264234.
HOVERGENiHBG108475.
InParanoidiQ8TBK2.
KOiK05302.
OMAiDEFITWL.
OrthoDBiEOG091G068E.
PhylomeDBiQ8TBK2.
TreeFamiTF106399.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000103037-MONOMER.
ReactomeiR-HSA-3214841. PKMTs methylate histone lysines.

Miscellaneous databases

ChiTaRSiSETD6. human.
GenomeRNAii79918.
PROiQ8TBK2.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000103037.
CleanExiHS_SETD6.
ExpressionAtlasiQ8TBK2. baseline and differential.
GenevisibleiQ8TBK2. HS.

Family and domain databases

Gene3Di3.90.1420.10. 1 hit.
InterProiIPR011383. N-lys_methylase_SETD6.
IPR015353. Rubisco_LSMT_subst-bd.
IPR001214. SET_dom.
[Graphical view]
PfamiPF09273. Rubis-subs-bind. 1 hit.
PF00856. SET. 1 hit.
[Graphical view]
PIRSFiPIRSF011771. RMS1_SET. 1 hit.
SUPFAMiSSF81822. SSF81822. 1 hit.
PROSITEiPS50280. SET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSETD6_HUMAN
AccessioniPrimary (citable) accession number: Q8TBK2
Secondary accession number(s): A8K380, B5ME38, Q9H787
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: October 5, 2010
Last modified: November 30, 2016
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.