Q8TBF2 (PGFS_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Prostamide/prostaglandin F synthase
Short name=Prostamide/PG F synthase
Short name=Prostamide/PGF synthase
EC=1.11.1.20

Alternative name(s):
Protein FAM213B

Gene names

Name:	FAM213B
Synonyms:	C1orf93

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	198 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the reduction of prostaglandin-ethanolamide H2 (prostamide H2) to prostamide F(2alpha) with NADPH as proton donor. Also able to reduce prostaglandin H2 to prostaglandin F(2alpha) By similarity.
Catalytic activity	Thioredoxin + prostaglandin H2 = thioredoxin disulfide + prostaglandin F(2-alpha).
Subcellular location	Cytoplasm › cytosol By similarity.
Sequence similarities	Belongs to the peroxiredoxin-like FAM213 family. prostamide/prostaglandin F synthase subfamily.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Fatty acid metabolism Lipid biosynthesis Lipid metabolism Prostaglandin biosynthesis Prostaglandin metabolism
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cyclooxygenase pathway Traceable author statement. Source: Reactome
Cellular_component	cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 7 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8TBF2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8TBF2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     154-198: GGDKVLLHFVQKSPGDYVPKEHILQVLGISAEVCASDPPQCDREV → EVPRRLRPQGAHPAGPGHLCGGLCQRPASV

Note: No experimental confirmation available.

Isoform 3 (identifier: Q8TBF2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     106-106: K → KRLWTQASPEFGQATWCLR

Note: No experimental confirmation available.

Isoform 4 (identifier: Q8TBF2-4)

The sequence of this isoform differs from the canonical sequence as follows:
     129-164: Missing.

Note: No experimental confirmation available.

Isoform 5 (identifier: Q8TBF2-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MSRERGSRSEEPGAGNRESGSREPGLAAAAM
     128-164: Missing.

Note: No experimental confirmation available.

Isoform 6 (identifier: Q8TBF2-6)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MSRERGSRSEEPGAGNRESGSREPGLAAAAM
     130-164: KAVGIQGNLSGDLLQSGGLLVVSKGGDKVLLHFVQ → VPTPDRPRLLASRGTCLGTCCRAEGCWWSAK

Note: No experimental confirmation available.

Isoform 7 (identifier: Q8TBF2-7)

The sequence of this isoform differs from the canonical sequence as follows:
     106-106: K → KRLWTQASPEFGQATWCLR
     154-198: GGDKVLLHFVQKSPGDYVPKEHILQVLGISAEVCASDPPQCDREV → EVPRRLRPQGAHPAGPGHLCGGLCQRPASV

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 198	198	Prostamide/prostaglandin F synthase		PRO_0000284637
Amino acid modifications
	Modified residue	108	1	Phosphotyrosine By similarity
Natural variations
	Alternative sequence	1	1	M → MSRERGSRSEEPGAGNRESG SREPGLAAAAM in isoform 5 and isoform 6.		VSP_040900
	Alternative sequence	106	1	K → KRLWTQASPEFGQATWCLR in isoform 3 and isoform 7.		VSP_040901
	Alternative sequence	128 – 164	37	Missing in isoform 5.		VSP_040902
	Alternative sequence	129 – 164	36	Missing in isoform 4.		VSP_040903
	Alternative sequence	130 – 164	35	KAVGI…LHFVQ → VPTPDRPRLLASRGTCLGTC CRAEGCWWSAK in isoform 6.		VSP_040904
	Alternative sequence	154 – 198	45	GGDKV…CDREV → EVPRRLRPQGAHPAGPGHLC GGLCQRPASV in isoform 2 and isoform 7.		VSP_024581

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 5E846BAFF44BA7FF	FASTA	198	21,223
	10 20 30 40 50 60 MSTVDLARVG ACILKHAVTG EAVELRSLWR EHACVVAGLR RFGCVVCRWI AQDLSSLAGL 70 80 90 100 110 120 LDQHGVRLVG VGPEALGLQE FLDGDYFAGE LYLDESKQLY KELGFKRYNS LSILPAALGK 130 140 150 160 170 180 PVRDVAAKAK AVGIQGNLSG DLLQSGGLLV VSKGGDKVLL HFVQKSPGDY VPKEHILQVL 190 GISAEVCASD PPQCDREV
	Isoform 2 [UniParc]. Checksum: FD28CF6BCD965AB4	FASTA	183	19,465
	10 20 30 40 50 60 MSTVDLARVG ACILKHAVTG EAVELRSLWR EHACVVAGLR RFGCVVCRWI AQDLSSLAGL 70 80 90 100 110 120 LDQHGVRLVG VGPEALGLQE FLDGDYFAGE LYLDESKQLY KELGFKRYNS LSILPAALGK 130 140 150 160 170 180 PVRDVAAKAK AVGIQGNLSG DLLQSGGLLV VSKEVPRRLR PQGAHPAGPG HLCGGLCQRP ASV
	Isoform 3 [UniParc]. Checksum: E48960669AE1407A	FASTA	216	23,355
	10 20 30 40 50 60 MSTVDLARVG ACILKHAVTG EAVELRSLWR EHACVVAGLR RFGCVVCRWI AQDLSSLAGL 70 80 90 100 110 120 LDQHGVRLVG VGPEALGLQE FLDGDYFAGE LYLDESKQLY KELGFKRLWT QASPEFGQAT 130 140 150 160 170 180 WCLRRYNSLS ILPAALGKPV RDVAAKAKAV GIQGNLSGDL LQSGGLLVVS KGGDKVLLHF 190 200 210 VQKSPGDYVP KEHILQVLGI SAEVCASDPP QCDREV
	Isoform 4 [UniParc]. Checksum: A86C8C59A5F7456D	FASTA	162	17,621
	10 20 30 40 50 60 MSTVDLARVG ACILKHAVTG EAVELRSLWR EHACVVAGLR RFGCVVCRWI AQDLSSLAGL 70 80 90 100 110 120 LDQHGVRLVG VGPEALGLQE FLDGDYFAGE LYLDESKQLY KELGFKRYNS LSILPAALGK 130 140 150 160 PVRDVAAKKS PGDYVPKEHI LQVLGISAEV CASDPPQCDR EV
	Isoform 5 [UniParc]. Checksum: AF2E4226898F0347	FASTA	191	20,548
	10 20 30 40 50 60 MSRERGSRSE EPGAGNRESG SREPGLAAAA MSTVDLARVG ACILKHAVTG EAVELRSLWR 70 80 90 100 110 120 EHACVVAGLR RFGCVVCRWI AQDLSSLAGL LDQHGVRLVG VGPEALGLQE FLDGDYFAGE 130 140 150 160 170 180 LYLDESKQLY KELGFKRYNS LSILPAALGK PVRDVAAKSP GDYVPKEHIL QVLGISAEVC 190 ASDPPQCDRE V
	Isoform 6 [UniParc]. Checksum: B34697403B2FE08B	FASTA	224	24,122
	10 20 30 40 50 60 MSRERGSRSE EPGAGNRESG SREPGLAAAA MSTVDLARVG ACILKHAVTG EAVELRSLWR 70 80 90 100 110 120 EHACVVAGLR RFGCVVCRWI AQDLSSLAGL LDQHGVRLVG VGPEALGLQE FLDGDYFAGE 130 140 150 160 170 180 LYLDESKQLY KELGFKRYNS LSILPAALGK PVRDVAAKAV PTPDRPRLLA SRGTCLGTCC 190 200 210 220 RAEGCWWSAK KSPGDYVPKE HILQVLGISA EVCASDPPQC DREV
	Isoform 7 [UniParc]. Checksum: 5AAB99C720FB6697	FASTA	201	21,597
	10 20 30 40 50 60 MSTVDLARVG ACILKHAVTG EAVELRSLWR EHACVVAGLR RFGCVVCRWI AQDLSSLAGL 70 80 90 100 110 120 LDQHGVRLVG VGPEALGLQE FLDGDYFAGE LYLDESKQLY KELGFKRLWT QASPEFGQAT 130 140 150 160 170 180 WCLRRYNSLS ILPAALGKPV RDVAAKAKAV GIQGNLSGDL LQSGGLLVVS KEVPRRLRPQ 190 200 GAHPAGPGHL CGGLCQRPAS V

References

[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2; 3; 4; 5 AND 6). Tissue: Neuroepithelioma, Teratocarcinoma, Thymus and Thyroid.
[2]	Guo J.H., She X.Y., Yu L. Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK057027 mRNA. Translation: BAG51845.1. AK075273 mRNA. Translation: BAC11511.1. AK291908 mRNA. Translation: BAF84597.1. AK298926 mRNA. Translation: BAG61031.1. AK303504 mRNA. Translation: BAG64537.1. AK316243 mRNA. Translation: BAH14614.1. AF425266 mRNA. Translation: AAP97295.1. AL139246 Genomic DNA. Translation: CAX30825.1. AL139246 Genomic DNA. Translation: CAX30826.1. AL139246 Genomic DNA. Translation: CAX30827.1. CH471183 Genomic DNA. Translation: EAW56086.1. CH471183 Genomic DNA. Translation: EAW56087.1. CH471183 Genomic DNA. Translation: EAW56088.1. BC022547 mRNA. Translation: AAH22547.1.
IPI	IPI00152199. IPI00383997. IPI00643409. IPI00908363. IPI00910916. IPI00976876. IPI01008862.
RefSeq	NP_001182665.1. NM_001195736.1. NP_001182666.1. NM_001195737.1. NP_001182667.1. NM_001195738.1. NP_001182669.1. NM_001195740.1. NP_001182670.1. NM_001195741.1. NP_689584.2. NM_152371.3.
UniGene	Hs.462033.
3D structure databases
ProteinModelPortal	Q8TBF2.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-8247583.
STRING	9606.ENSP00000367682.
PTM databases
PhosphoSite	Q8TBF2.
Polymorphism databases
DMDM	74760424.
Proteomic databases
PaxDb	Q8TBF2.
PRIDE	Q8TBF2.
Protocols and materials databases
DNASU	127281.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000378425; ENSP00000367682; ENSG00000157870. ENST00000444521; ENSP00000413218; ENSG00000157870. ENST00000537325; ENSP00000443605; ENSG00000157870.
GeneID	127281.
KEGG	hsa:127281.
UCSC	uc001aju.2. human. uc001ajv.2. human. uc001ajw.2. human. uc010nzd.2. human. uc010nze.2. human. uc010nzf.2. human.
Organism-specific databases
CTD	127281.
GeneCards	GC01P002517.
HGNC	HGNC:28390. FAM213B.
HPA	HPA006403.
neXtProt	NX_Q8TBF2.
PharmGKB	PA142672477.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG276778.
HOVERGEN	HBG106494.
KO	K15717.
OrthoDB	EOG42822R.
PhylomeDB	Q8TBF2.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism.
Gene expression databases
Bgee	Q8TBF2.
CleanEx	HS_C1orf93.
Genevestigator	Q8TBF2.
Family and domain databases
ProtoNet	Search...
Other
GenomeRNAi	127281.
NextBio	82055.

Entry information

Entry name

PGFS_HUMAN

Accession

Primary (citable) accession number: Q8TBF2
Secondary accession number(s): A8K793 , B3KPY3, B4DQR9, B4E0S5, B7ZAC8, B9DI90, B9DI92, Q8N2H0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 17, 2007
Last sequence update:	June 1, 2002
Last modified:	May 29, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.