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Protein

SH3KBP1-binding protein 1

Gene

SHKBP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits CBL-SH3KBP1 complex mediated down-regulation of EGFR signaling by sequestration of SH3KBP1. Binds to SH3KBP1 and prevents its interaction with CBL and inhibits translocation of SH3KBP1 to EGFR containing vesicles upon EGF stimulation.By similarity

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
SH3KBP1-binding protein 1
Gene namesi
Name:SHKBP1
ORF Names:PP203
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:19214. SHKBP1.

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134954640.

Polymorphism and mutation databases

BioMutaiSHKBP1.
DMDMi160185660.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 707706SH3KBP1-binding protein 1PRO_0000307932Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineCombined sources
Modified residuei649 – 6491PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8TBC3.
MaxQBiQ8TBC3.
PaxDbiQ8TBC3.
PRIDEiQ8TBC3.

PTM databases

iPTMnetiQ8TBC3.
PhosphoSiteiQ8TBC3.

Expressioni

Gene expression databases

BgeeiQ8TBC3.
CleanExiHS_SHKBP1.
ExpressionAtlasiQ8TBC3. baseline and differential.
GenevisibleiQ8TBC3. HS.

Organism-specific databases

HPAiHPA041887.

Interactioni

Subunit structurei

Interacts (via PXXXPR motifs) with SH3KBP1 (via SH3 domains).By similarity

Protein-protein interaction databases

BioGridi124979. 32 interactions.
IntActiQ8TBC3. 11 interactions.
MINTiMINT-1397700.
STRINGi9606.ENSP00000291842.

Structurei

Secondary structure

1
707
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi20 – 256Combined sources
Beta strandi28 – 336Combined sources
Helixi34 – 385Combined sources
Helixi44 – 485Combined sources
Beta strandi63 – 653Combined sources
Helixi72 – 8110Combined sources
Helixi93 – 1019Combined sources
Helixi104 – 11714Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4CRHX-ray1.72A18-120[»]
ProteinModelPortaliQ8TBC3.
SMRiQ8TBC3. Positions 18-120.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini19 – 8870BTBPROSITE-ProRule annotationAdd
BLAST
Repeati185 – 22541WD 1Add
BLAST
Repeati226 – 27853WD 2Add
BLAST
Repeati279 – 31840WD 3Add
BLAST
Repeati319 – 36244WD 4Add
BLAST
Repeati363 – 42058WD 5Add
BLAST
Repeati421 – 46646WD 6Add
BLAST
Repeati467 – 51852WD 7Add
BLAST
Repeati519 – 57759WD 8Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi621 – 6266PXXXPRCurated
Motifi681 – 6866PXXXPWCurated

Sequence similaritiesi

Belongs to the KCTD3 family.Curated
Contains 1 BTB (POZ) domain.PROSITE-ProRule annotation
Contains 8 WD repeats.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG2714. Eukaryota.
ENOG410XSWI. LUCA.
GeneTreeiENSGT00530000062980.
HOGENOMiHOG000006656.
HOVERGENiHBG061001.
InParanoidiQ8TBC3.
OMAiIQKVVPN.
OrthoDBiEOG7P8P7H.
PhylomeDBiQ8TBC3.
TreeFamiTF313754.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR000210. BTB/POZ_dom.
IPR011333. SKP1/BTB/POZ.
IPR003131. T1-type_BTB.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF02214. BTB_2. 1 hit.
[Graphical view]
SMARTiSM00225. BTB. 1 hit.
SM00320. WD40. 3 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
SSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
PS00678. WD_REPEATS_1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8TBC3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAATAAEG VPSRGPPGEV IHLNVGGKRF STSRQTLTWI PDSFFSSLLS
60 70 80 90 100
GRISTLKDET GAIFIDRDPT VFAPILNFLR TKELDPRGVH GSSLLHEAQF
110 120 130 140 150
YGLTPLVRRL QLREELDRSS CGNVLFNGYL PPPVFPVKRR NRHSLVGPQQ
160 170 180 190 200
LGGRPAPVRR SNTMPPNLGN AGLLGRMLDE KTPPSPSGQP EEPGMVRLVC
210 220 230 240 250
GHHNWIAVAY TQFLVCYRLK EASGWQLVFS SPRLDWPIER LALTARVHGG
260 270 280 290 300
ALGEHDKMVA AATGSEILLW ALQAEGGGSE IGVFHLGVPV EALFFVGNQL
310 320 330 340 350
IATSHTGRIG VWNAVTKHWQ VQEVQPITSY DAAGSFLLLG CNNGSIYYVD
360 370 380 390 400
VQKFPLRMKD NDLLVSELYR DPAEDGVTAL SVYLTPKTSD SGNWIEIAYG
410 420 430 440 450
TSSGGVRVIV QHPETVGSGP QLFQTFTVHR SPVTKIMLSE KHLISVCADN
460 470 480 490 500
NHVRTWSVTR FRGMISTQPG STPLASFKIL ALESADGHGG CSAGNDIGPY
510 520 530 540 550
GERDDQQVFI QKVVPSASQL FVRLSSTGQR VCSVRSVDGS PTTAFTVLEC
560 570 580 590 600
EGSRRLGSRP RRYLLTGQAN GSLAMWDLTT AMDGLGQAPA GGLTEQELME
610 620 630 640 650
QLEHCELAPP APSAPSWGCL PSPSPRISLT SLHSASSNTS LSGHRGSPSP
660 670 680 690 700
PQAEARRRGG GSFVERCQEL VRSGPDLRRP PTPAPWPSSG LGTPLTPPKM

KLNETSF
Length:707
Mass (Da):76,344
Last modified:October 23, 2007 - v2
Checksum:i1E38F2FA5B84332F
GO
Isoform 2 (identifier: Q8TBC3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     283-305: VFHLGVPVEALFFVGNQLIATSH → AHRGVECRHQALAGPGGAAHHQL
     306-707: Missing.

Note: No experimental confirmation available.
Show »
Length:305
Mass (Da):33,024
Checksum:i814F358C32D8FF65
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti58 – 581D → G in BAC11374 (PubMed:14702039).Curated
Sequence conflicti153 – 1531G → R in BAC11374 (PubMed:14702039).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti507 – 5071Q → L.1 Publication
Corresponds to variant rs17855499 [ dbSNP | Ensembl ].
VAR_036714

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei283 – 30523VFHLG…IATSH → AHRGVECRHQALAGPGGAAH HQL in isoform 2. 1 PublicationVSP_028873Add
BLAST
Alternative sequencei306 – 707402Missing in isoform 2. 1 PublicationVSP_028874Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK075057 mRNA. Translation: BAC11374.1.
AF258553 mRNA. Translation: AAG23756.1.
BC007653 mRNA. Translation: AAH07653.1.
BC022855 mRNA. Translation: AAH22855.1.
CCDSiCCDS12560.1. [Q8TBC3-1]
RefSeqiNP_612401.2. NM_138392.3. [Q8TBC3-1]
UniGeneiHs.26506.

Genome annotation databases

EnsembliENST00000291842; ENSP00000291842; ENSG00000160410. [Q8TBC3-1]
GeneIDi92799.
KEGGihsa:92799.
UCSCiuc002oob.4. human. [Q8TBC3-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK075057 mRNA. Translation: BAC11374.1.
AF258553 mRNA. Translation: AAG23756.1.
BC007653 mRNA. Translation: AAH07653.1.
BC022855 mRNA. Translation: AAH22855.1.
CCDSiCCDS12560.1. [Q8TBC3-1]
RefSeqiNP_612401.2. NM_138392.3. [Q8TBC3-1]
UniGeneiHs.26506.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4CRHX-ray1.72A18-120[»]
ProteinModelPortaliQ8TBC3.
SMRiQ8TBC3. Positions 18-120.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124979. 32 interactions.
IntActiQ8TBC3. 11 interactions.
MINTiMINT-1397700.
STRINGi9606.ENSP00000291842.

PTM databases

iPTMnetiQ8TBC3.
PhosphoSiteiQ8TBC3.

Polymorphism and mutation databases

BioMutaiSHKBP1.
DMDMi160185660.

Proteomic databases

EPDiQ8TBC3.
MaxQBiQ8TBC3.
PaxDbiQ8TBC3.
PRIDEiQ8TBC3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000291842; ENSP00000291842; ENSG00000160410. [Q8TBC3-1]
GeneIDi92799.
KEGGihsa:92799.
UCSCiuc002oob.4. human. [Q8TBC3-1]

Organism-specific databases

CTDi92799.
GeneCardsiSHKBP1.
HGNCiHGNC:19214. SHKBP1.
HPAiHPA041887.
neXtProtiNX_Q8TBC3.
PharmGKBiPA134954640.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2714. Eukaryota.
ENOG410XSWI. LUCA.
GeneTreeiENSGT00530000062980.
HOGENOMiHOG000006656.
HOVERGENiHBG061001.
InParanoidiQ8TBC3.
OMAiIQKVVPN.
OrthoDBiEOG7P8P7H.
PhylomeDBiQ8TBC3.
TreeFamiTF313754.

Miscellaneous databases

GenomeRNAii92799.
PROiQ8TBC3.

Gene expression databases

BgeeiQ8TBC3.
CleanExiHS_SHKBP1.
ExpressionAtlasiQ8TBC3. baseline and differential.
GenevisibleiQ8TBC3. HS.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR000210. BTB/POZ_dom.
IPR011333. SKP1/BTB/POZ.
IPR003131. T1-type_BTB.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF02214. BTB_2. 1 hit.
[Graphical view]
SMARTiSM00225. BTB. 1 hit.
SM00320. WD40. 3 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
SSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
PS00678. WD_REPEATS_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Ovary.
  2. "Large-scale cDNA transfection screening for genes related to cancer development and progression."
    Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X.
    , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J.
    Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT LEU-507.
    Tissue: Brain and Uterus.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-649, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  5. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  6. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSHKB1_HUMAN
AccessioniPrimary (citable) accession number: Q8TBC3
Secondary accession number(s): Q8N2I6, Q8WY93, Q96IB8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: October 23, 2007
Last modified: June 8, 2016
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.