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Protein

SH3KBP1-binding protein 1

Gene

SHKBP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits CBL-SH3KBP1 complex mediated down-regulation of EGFR signaling by sequestration of SH3KBP1. Binds to SH3KBP1 and prevents its interaction with CBL and inhibits translocation of SH3KBP1 to EGFR containing vesicles upon EGF stimulation.By similarity

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
SH3KBP1-binding protein 1
Alternative name(s):
SETA-binding protein 11 Publication
Gene namesi
Name:SHKBP1
Synonyms:SB11 Publication
ORF Names:PP203
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000160410.14.
HGNCiHGNC:19214. SHKBP1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Organism-specific databases

DisGeNETi92799.
OpenTargetsiENSG00000160410.
PharmGKBiPA134954640.

Polymorphism and mutation databases

BioMutaiSHKBP1.
DMDMi160185660.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00003079322 – 707SH3KBP1-binding protein 1Add BLAST706

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei163PhosphothreonineCombined sources1
Modified residuei647PhosphoserineCombined sources1
Modified residuei649PhosphoserineCombined sources1
Modified residuei696PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8TBC3.
MaxQBiQ8TBC3.
PaxDbiQ8TBC3.
PeptideAtlasiQ8TBC3.
PRIDEiQ8TBC3.

PTM databases

iPTMnetiQ8TBC3.
PhosphoSitePlusiQ8TBC3.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiENSG00000160410.
CleanExiHS_SHKBP1.
ExpressionAtlasiQ8TBC3. baseline and differential.
GenevisibleiQ8TBC3. HS.

Organism-specific databases

HPAiHPA041887.

Interactioni

Subunit structurei

Interacts (via PXXXPR motifs) with SH3KBP1 (via SH3 domains) (By similarity). Directly interacts with cathepsin B/CTSB (PubMed:16733801).By similarity1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi124979. 42 interactors.
IntActiQ8TBC3. 15 interactors.
MINTiMINT-1397700.
STRINGi9606.ENSP00000291842.

Structurei

Secondary structure

1707
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi20 – 25Combined sources6
Beta strandi28 – 33Combined sources6
Helixi34 – 38Combined sources5
Helixi44 – 48Combined sources5
Beta strandi63 – 65Combined sources3
Helixi72 – 81Combined sources10
Helixi93 – 101Combined sources9
Helixi104 – 117Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4CRHX-ray1.72A18-120[»]
ProteinModelPortaliQ8TBC3.
SMRiQ8TBC3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini19 – 88BTBPROSITE-ProRule annotationAdd BLAST70
Repeati185 – 225WD 1Add BLAST41
Repeati226 – 278WD 2Add BLAST53
Repeati279 – 318WD 3Add BLAST40
Repeati319 – 362WD 4Add BLAST44
Repeati363 – 420WD 5Add BLAST58
Repeati421 – 466WD 6Add BLAST46
Repeati467 – 518WD 7Add BLAST52
Repeati519 – 577WD 8Add BLAST59

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi621 – 626PXXXPRCurated6
Motifi681 – 686PXXXPWCurated6

Sequence similaritiesi

Belongs to the KCTD3 family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG2714. Eukaryota.
ENOG410XSWI. LUCA.
GeneTreeiENSGT00530000062980.
HOGENOMiHOG000006656.
HOVERGENiHBG061001.
InParanoidiQ8TBC3.
KOiK21953.
OMAiFPVKRRN.
OrthoDBiEOG091G0391.
PhylomeDBiQ8TBC3.
TreeFamiTF313754.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiView protein in InterPro
IPR000210. BTB/POZ_dom.
IPR011333. SKP1/BTB/POZ.
IPR003131. T1-type_BTB.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR036322. WD40_repeat_dom_sf.
PfamiView protein in Pfam
PF02214. BTB_2. 1 hit.
SMARTiView protein in SMART
SM00225. BTB. 1 hit.
SM00320. WD40. 3 hits.
SUPFAMiSSF50978. SSF50978. 2 hits.
SSF54695. SSF54695. 1 hit.
PROSITEiView protein in PROSITE
PS50097. BTB. 1 hit.
PS00678. WD_REPEATS_1. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8TBC3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAATAAEG VPSRGPPGEV IHLNVGGKRF STSRQTLTWI PDSFFSSLLS
60 70 80 90 100
GRISTLKDET GAIFIDRDPT VFAPILNFLR TKELDPRGVH GSSLLHEAQF
110 120 130 140 150
YGLTPLVRRL QLREELDRSS CGNVLFNGYL PPPVFPVKRR NRHSLVGPQQ
160 170 180 190 200
LGGRPAPVRR SNTMPPNLGN AGLLGRMLDE KTPPSPSGQP EEPGMVRLVC
210 220 230 240 250
GHHNWIAVAY TQFLVCYRLK EASGWQLVFS SPRLDWPIER LALTARVHGG
260 270 280 290 300
ALGEHDKMVA AATGSEILLW ALQAEGGGSE IGVFHLGVPV EALFFVGNQL
310 320 330 340 350
IATSHTGRIG VWNAVTKHWQ VQEVQPITSY DAAGSFLLLG CNNGSIYYVD
360 370 380 390 400
VQKFPLRMKD NDLLVSELYR DPAEDGVTAL SVYLTPKTSD SGNWIEIAYG
410 420 430 440 450
TSSGGVRVIV QHPETVGSGP QLFQTFTVHR SPVTKIMLSE KHLISVCADN
460 470 480 490 500
NHVRTWSVTR FRGMISTQPG STPLASFKIL ALESADGHGG CSAGNDIGPY
510 520 530 540 550
GERDDQQVFI QKVVPSASQL FVRLSSTGQR VCSVRSVDGS PTTAFTVLEC
560 570 580 590 600
EGSRRLGSRP RRYLLTGQAN GSLAMWDLTT AMDGLGQAPA GGLTEQELME
610 620 630 640 650
QLEHCELAPP APSAPSWGCL PSPSPRISLT SLHSASSNTS LSGHRGSPSP
660 670 680 690 700
PQAEARRRGG GSFVERCQEL VRSGPDLRRP PTPAPWPSSG LGTPLTPPKM

KLNETSF
Length:707
Mass (Da):76,344
Last modified:October 23, 2007 - v2
Checksum:i1E38F2FA5B84332F
GO
Isoform 2 (identifier: Q8TBC3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     283-305: VFHLGVPVEALFFVGNQLIATSH → AHRGVECRHQALAGPGGAAHHQL
     306-707: Missing.

Note: No experimental confirmation available.
Show »
Length:305
Mass (Da):33,024
Checksum:i814F358C32D8FF65
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti58D → G in BAC11374 (PubMed:14702039).Curated1
Sequence conflicti153G → R in BAC11374 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_036714507Q → L1 PublicationCorresponds to variant dbSNP:rs17855499Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_028873283 – 305VFHLG…IATSH → AHRGVECRHQALAGPGGAAH HQL in isoform 2. 1 PublicationAdd BLAST23
Alternative sequenceiVSP_028874306 – 707Missing in isoform 2. 1 PublicationAdd BLAST402

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK075057 mRNA. Translation: BAC11374.1.
AF258553 mRNA. Translation: AAG23756.1.
BC007653 mRNA. Translation: AAH07653.1.
BC022855 mRNA. Translation: AAH22855.1.
CCDSiCCDS12560.1. [Q8TBC3-1]
RefSeqiNP_612401.2. NM_138392.3. [Q8TBC3-1]
UniGeneiHs.26506.

Genome annotation databases

EnsembliENST00000291842; ENSP00000291842; ENSG00000160410. [Q8TBC3-1]
GeneIDi92799.
KEGGihsa:92799.
UCSCiuc002oob.4. human. [Q8TBC3-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiSHKB1_HUMAN
AccessioniPrimary (citable) accession number: Q8TBC3
Secondary accession number(s): Q8N2I6, Q8WY93, Q96IB8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: October 23, 2007
Last modified: October 25, 2017
This is version 132 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families