Reviewed, UniProtKB/Swiss-Prot Q8TB72 (PUM2_HUMAN)

Names and origin

Protein names

Recommended name:
    Pumilio homolog 2
      Short name=Pumilio-2

Gene names

Name:	PUM2
Synonyms:	KIAA0235, PUMH2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1066 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts.
Subunit structure	Homodimer; homodimerizes in vitro. Interacts with DAZ, DAZL and NANOS1 via its pumilio repeats. Binds to a RNA consensus sequence, that is related to the Nanos Response Element (NRE), a 16 bp sequence found in the 3'-UTR of the Drosophila hb mRNA. Also binds to the NRE. Ref.6 Ref.8
Subcellular location	Cytoplasm Probable.
Tissue specificity	Highly expressed in testis and ovary. Predominantly expressed in stem cells and germ cells. Expressed at lower level in brain, heart, kidney, liver, muscle, placenta, intestine and stomach Expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen. Expressed in all fetal tissues tested. Ref.6 Ref.1
Induction	Down-regulated in keratinocytes upon UV B radiation. Ref.7
Domain	The pumilio repeats mediate the association with RNA by packing together to form a right-handed superhelix that approximates a half donut. The number as well as the specific sequence of the repeats determine the specificity for target mRNAs By similarity.
Sequence similarities	Contains 1 PUM-HD domain. Contains 8 pumilio repeats.

Ontologies

Keywords
Biological process	Translation regulation
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat
Ligand	RNA-binding
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Ref.6 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
DAZ1	Q9NQZ3	4	EBI-311190,EBI-997955

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8TB72-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8TB72-2)

The sequence of this isoform differs from the canonical sequence as follows:
     574-652: Missing.

Isoform 3 (identifier: Q8TB72-3)

The sequence of this isoform differs from the canonical sequence as follows:
     829-830: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1066	1066	Pumilio homolog 2		PRO_0000075919
Regions
	Domain	706 – 1048	343	PUM-HD
	Repeat	726 – 761	36	Pumilio 1
	Repeat	762 – 797	36	Pumilio 2
	Repeat	798 – 835	38	Pumilio 3
	Repeat	836 – 871	36	Pumilio 4
	Repeat	872 – 907	36	Pumilio 5
	Repeat	908 – 943	36	Pumilio 6
	Repeat	944 – 979	36	Pumilio 7
	Repeat	983 – 1022	40	Pumilio 8
	Compositional bias	278 – 488	211	Ala-rich
	Compositional bias	359 – 405	47	Gln-rich
	Compositional bias	520 – 687	168	Ser-rich
Amino acid modifications
	Modified residue	82	1	Phosphoserine Ref.11 Ref.12
	Modified residue	136	1	Phosphoserine Ref.12 Ref.10
	Modified residue	178	1	Phosphoserine Ref.12
	Modified residue	182	1	Phosphoserine Ref.12 Ref.9
	Modified residue	184	1	Phosphothreonine Ref.12
	Modified residue	587	1	Phosphoserine Ref.12
Natural variations
	Alternative sequence	574 – 652	79	Missing in isoform 2.		VSP_009319
	Alternative sequence	829 – 830	2	Missing in isoform 3.		VSP_009320
	Natural variant	367	1	N → S: dbSNP rs34032508.		VAR_057100

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 2, 2004. Version 2. Checksum: CF6F9D7998CD5D9E	FASTA	1,066	114,216
	10 20 30 40 50 60 MNHDFQALAL ESRGMGELLP TKKFWEPDDS TKDGQKGIFL GDDEWRETAW GASHHSMSQP 70 80 90 100 110 120 IMVQRRSGQG FHGNSEVNAI LSPRSESGGL GVSMVEYVLS SSPADKLDSR FRKGNFGTRD 130 140 150 160 170 180 AETDGPEKGD QKGKASPFEE DQNRDLKQGD DDDSKINGRG LPNGMDADCK DFNRTPGSRQ 190 200 210 220 230 240 ASPTEVVERL GPNTNPSEGL GPLPNPTANK PLVEEFSNPE TQNLDAMEQV GLESLQFDYP 250 260 270 280 290 300 GNQVPMDSSG ATVGLFDYNS QQQLFQRTNA LTVQQLTAAQ QQQYALAAAQ QPHIAGVFSA 310 320 330 340 350 360 GLAPAAFVPN PYIISAAPPG TDPYTAAGLA AAATLAGPAV VPPQYYGVPW GVYPANLFQQ 370 380 390 400 410 420 QAAAAANNTA SQQAASQAQP GQQQVLRAGA GQRPLTPNQG QQGQQAESLA AAAAANPTLA 430 440 450 460 470 480 FGQGLATGMP GYQVLAPTAY YDQTGALVVG PGARTGLGAP VRLMAPTPVL ISSAAAQAAA 490 500 510 520 530 540 AAAAGGTASS LTGSTNGLFR PIGTQPPQQQ QQQPSTNLQS NSFYGSSSLT NSSQSSSLFS 550 560 570 580 590 600 HGPGQPGSTS LGFGSGNSLG AAIGSALSGF GSSVGSSASS SATRRESLST SSDLYKRSSS 610 620 630 640 650 660 SLAPIGQPFY NSLGFSSSPS PIGMPLPSQT PGHSLTPPPS LSSHGSSSSL HLGGLTNGSG 670 680 690 700 710 720 RYISAAPGAE AKYRSASSTS SLFSSSSQLF PPSRLRYNRS DIMPSGRSRL LEDFRNNRFP 730 740 750 760 770 780 NLQLRDLIGH IVEFSQDQHG SRFIQQKLER ATPAERQMVF NEILQAAYQL MTDVFGNYVI 790 800 810 820 830 840 QKFFEFGSLD QKLALATRIR GHVLPLALQM YGCRVIQKAL ESISSDQQVI SEMVKELDGH 850 860 870 880 890 900 VLKCVKDQNG NHVVQKCIEC VQPQSLQFII DAFKGQVFVL STHPYGCRVI QRILEHCTAE 910 920 930 940 950 960 QTLPILEELH QHTEQLVQDQ YGNYVIQHVL EHGRPEDKSK IVSEIRGKVL ALSQHKFASN 970 980 990 1000 1010 1020 VVEKCVTHAS RAERALLIDE VCCQNDGPHS ALYTMMKDQY ANYVVQKMID MAEPAQRKII 1030 1040 1050 1060 MHKIRPHITT LRKYTYGKHI LAKLEKYYLK NSPDLGPIGG PPNGML
	Isoform 2. Checksum: 99F119F5E3CFCCCF	FASTA	987	106,215
	10 20 30 40 50 60 MNHDFQALAL ESRGMGELLP TKKFWEPDDS TKDGQKGIFL GDDEWRETAW GASHHSMSQP 70 80 90 100 110 120 IMVQRRSGQG FHGNSEVNAI LSPRSESGGL GVSMVEYVLS SSPADKLDSR FRKGNFGTRD 130 140 150 160 170 180 AETDGPEKGD QKGKASPFEE DQNRDLKQGD DDDSKINGRG LPNGMDADCK DFNRTPGSRQ 190 200 210 220 230 240 ASPTEVVERL GPNTNPSEGL GPLPNPTANK PLVEEFSNPE TQNLDAMEQV GLESLQFDYP 250 260 270 280 290 300 GNQVPMDSSG ATVGLFDYNS QQQLFQRTNA LTVQQLTAAQ QQQYALAAAQ QPHIAGVFSA 310 320 330 340 350 360 GLAPAAFVPN PYIISAAPPG TDPYTAAGLA AAATLAGPAV VPPQYYGVPW GVYPANLFQQ 370 380 390 400 410 420 QAAAAANNTA SQQAASQAQP GQQQVLRAGA GQRPLTPNQG QQGQQAESLA AAAAANPTLA 430 440 450 460 470 480 FGQGLATGMP GYQVLAPTAY YDQTGALVVG PGARTGLGAP VRLMAPTPVL ISSAAAQAAA 490 500 510 520 530 540 AAAAGGTASS LTGSTNGLFR PIGTQPPQQQ QQQPSTNLQS NSFYGSSSLT NSSQSSSLFS 550 560 570 580 590 600 HGPGQPGSTS LGFGSGNSLG AAIGSALSGF GSSGGLTNGS GRYISAAPGA EAKYRSASST 610 620 630 640 650 660 SSLFSSSSQL FPPSRLRYNR SDIMPSGRSR LLEDFRNNRF PNLQLRDLIG HIVEFSQDQH 670 680 690 700 710 720 GSRFIQQKLE RATPAERQMV FNEILQAAYQ LMTDVFGNYV IQKFFEFGSL DQKLALATRI 730 740 750 760 770 780 RGHVLPLALQ MYGCRVIQKA LESISSDQQV ISEMVKELDG HVLKCVKDQN GNHVVQKCIE 790 800 810 820 830 840 CVQPQSLQFI IDAFKGQVFV LSTHPYGCRV IQRILEHCTA EQTLPILEEL HQHTEQLVQD 850 860 870 880 890 900 QYGNYVIQHV LEHGRPEDKS KIVSEIRGKV LALSQHKFAS NVVEKCVTHA SRAERALLID 910 920 930 940 950 960 EVCCQNDGPH SALYTMMKDQ YANYVVQKMI DMAEPAQRKI IMHKIRPHIT TLRKYTYGKH 970 980 ILAKLEKYYL KNSPDLGPIG GPPNGML
	Isoform 3. Checksum: 99BC2A2711306F18	FASTA	1,064	114,003
	10 20 30 40 50 60 MNHDFQALAL ESRGMGELLP TKKFWEPDDS TKDGQKGIFL GDDEWRETAW GASHHSMSQP 70 80 90 100 110 120 IMVQRRSGQG FHGNSEVNAI LSPRSESGGL GVSMVEYVLS SSPADKLDSR FRKGNFGTRD 130 140 150 160 170 180 AETDGPEKGD QKGKASPFEE DQNRDLKQGD DDDSKINGRG LPNGMDADCK DFNRTPGSRQ 190 200 210 220 230 240 ASPTEVVERL GPNTNPSEGL GPLPNPTANK PLVEEFSNPE TQNLDAMEQV GLESLQFDYP 250 260 270 280 290 300 GNQVPMDSSG ATVGLFDYNS QQQLFQRTNA LTVQQLTAAQ QQQYALAAAQ QPHIAGVFSA 310 320 330 340 350 360 GLAPAAFVPN PYIISAAPPG TDPYTAAGLA AAATLAGPAV VPPQYYGVPW GVYPANLFQQ 370 380 390 400 410 420 QAAAAANNTA SQQAASQAQP GQQQVLRAGA GQRPLTPNQG QQGQQAESLA AAAAANPTLA 430 440 450 460 470 480 FGQGLATGMP GYQVLAPTAY YDQTGALVVG PGARTGLGAP VRLMAPTPVL ISSAAAQAAA 490 500 510 520 530 540 AAAAGGTASS LTGSTNGLFR PIGTQPPQQQ QQQPSTNLQS NSFYGSSSLT NSSQSSSLFS 550 560 570 580 590 600 HGPGQPGSTS LGFGSGNSLG AAIGSALSGF GSSVGSSASS SATRRESLST SSDLYKRSSS 610 620 630 640 650 660 SLAPIGQPFY NSLGFSSSPS PIGMPLPSQT PGHSLTPPPS LSSHGSSSSL HLGGLTNGSG 670 680 690 700 710 720 RYISAAPGAE AKYRSASSTS SLFSSSSQLF PPSRLRYNRS DIMPSGRSRL LEDFRNNRFP 730 740 750 760 770 780 NLQLRDLIGH IVEFSQDQHG SRFIQQKLER ATPAERQMVF NEILQAAYQL MTDVFGNYVI 790 800 810 820 830 840 QKFFEFGSLD QKLALATRIR GHVLPLALQM YGCRVIQKAL ESISSDQQSE MVKELDGHVL 850 860 870 880 890 900 KCVKDQNGNH VVQKCIECVQ PQSLQFIIDA FKGQVFVLST HPYGCRVIQR ILEHCTAEQT 910 920 930 940 950 960 LPILEELHQH TEQLVQDQYG NYVIQHVLEH GRPEDKSKIV SEIRGKVLAL SQHKFASNVV 970 980 990 1000 1010 1020 EKCVTHASRA ERALLIDEVC CQNDGPHSAL YTMMKDQYAN YVVQKMIDMA EPAQRKIIMH 1030 1040 1050 1060 KIRPHITTLR KYTYGKHILA KLEKYYLKNS PDLGPIGGPP NGML

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and comparative sequence analysis of PUM1 and PUM2 genes, human members of the Pumilio family of RNA-binding proteins." Spassov D.S., Jurecic R. Gene 299:195-204(2002) [PubMed: 12459267] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), TISSUE SPECIFICITY.
[2]	"Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain." Nagase T., Seki N., Ishikawa K., Ohira M., Kawarabayasi Y., Ohara O., Tanaka A., Kotani H., Miyajima N., Nomura N. DNA Res. 3:321-329(1996) [PubMed: 9039502] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Brain.
[3]	"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones." Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T. DNA Res. 9:99-106(2002) [PubMed: 12168954] [Abstract] Cited for: SEQUENCE REVISION.
[4]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 578-1066 (ISOFORM 1). Tissue: Lung and Placenta.
[6]	"Human Pumilio-2 is expressed in embryonic stem cells and germ cells and interacts with DAZ (Deleted in AZoospermia) and DAZ-like proteins." Moore F.L., Jaruzelska J., Fox M.S., Urano J., Firpo M.T., Turek P.J., Dorfman D.M., Reijo Pera R.A. Proc. Natl. Acad. Sci. U.S.A. 100:538-543(2003) [PubMed: 12511597] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 110-1066 (ISOFORM 2), TISSUE SPECIFICITY, RNA-BINDING, INTERACTION WITH DAZ AND DAZL.
[7]	"Genome-wide comparison of human keratinocyte and squamous cell carcinoma responses to UVB irradiation: implications for skin and epithelial cancer." Dazard J.-E., Gal H., Amariglio N., Rechavi G., Domany E., Givol D. Oncogene 22:2993-3006(2003) [PubMed: 12771951] [Abstract] Cited for: INDUCTION.
[8]	"Conservation of a Pumilio-Nanos complex from Drosophila germ plasm to human germ cells." Jaruzelska J., Kotecki M., Kusz K., Spik A., Firpo M., Reijo Pera R.A. Dev. Genes Evol. 213:120-126(2003) [PubMed: 12690449] [Abstract] Cited for: HOMODIMERIZATION, INTERACTION WITH NANOS1.
[9]	"Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-182, MASS SPECTROMETRY. Tissue: Epithelium.
[10]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-136, MASS SPECTROMETRY. Tissue: Epithelium.
[11]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-82, MASS SPECTROMETRY.
[12]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-82; SER-136; SER-178; SER-182; THR-184 AND SER-587, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF315591 mRNA. Translation: AAG31806.1. D87078 mRNA. Translation: BAA19665.3. AC007041 Genomic DNA. Translation: AAY15026.1. BC024218 mRNA. Translation: AAH24218.2. BC112046 mRNA. Translation: AAI12047.1. BC112048 mRNA. Translation: AAI12049.1. AF272350 mRNA. Translation: AAL36981.1.
IPI	IPI00022184. IPI00398749. IPI00398750.
RefSeq	NP_056132.1.
UniGene	Hs.467824
3D structure databases
HSSP	HSSP built from PDB template 1M8Y based on UniProtKB Q14671.
SMR	Q8TB72. Positions 706-1045.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8TB72. 2 interactions.
PTM databases
PhosphoSite	Q8TB72.
Proteomic databases
PRIDE	Q8TB72.
Genome annotation databases
Ensembl	ENSG00000055917. Homo sapiens. [Contig view]
GeneID	23369.
KEGG	hsa:23369.
Organism-specific databases
GeneCards	GC02M020311.
H-InvDB	HIX0001857.
HGNC	HGNC:14958. PUM2.
MIM	607205. gene.
PharmGKB	PA34043.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q8TB72.
HOVERGEN	Q8TB72.
OMA	Q8TB72. FDYSSQQ.
Gene expression databases
ArrayExpress	Q8TB72.
Bgee	Q8TB72.
CleanEx	HS_PUM2.
GermOnline	ENSG00000055917. Homo sapiens.
Family and domain databases
InterPro	IPR011989. ARM-like. IPR001313. Pumilio_RNA_bd. [Graphical view]
Gene3D	G3DSA:1.25.10.10. ARM-like. 1 hit.
Pfam	PF00806. PUF. 8 hits. [Graphical view]
SMART	SM00025. Pumilio. 8 hits. [Graphical view]
PROSITE	PS50302. PUM. 8 hits. PS50303. PUM_HD. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	45440.
SOURCE	Search...

Entry information

Entry name

PUM2_HUMAN

Accession

Primary (citable) accession number: Q8TB72
Secondary accession number(s): O00234 , Q53TV7, Q8WY43, Q9HAN2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 2, 2004
Last sequence update:	February 2, 2004
Last modified:	June 16, 2009
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)