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Q8TB61 (S35B2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Adenosine 3'-phospho 5'-phosphosulfate transporter 1
Alternative name(s):
PAPS transporter 1
Putative MAPK-activating protein PM15
Putative NF-kappa-B-activating protein 48
Solute carrier family 35 member B2
Gene names
Name:SLC35B2
Synonyms:PAPST1
ORF Names:PSEC0149
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length432 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mediates the transport of adenosine 3'-phospho 5'-phosphosulfate (PAPS), from cytosol into Golgi. PAPS is a universal sulfuryl donor for sulfation events that take place in the Golgi. May indirectly participate in activation of the NF-kappa-B and MAPK pathways. Ref.1

Subcellular location

Golgi apparatus membrane; Multi-pass membrane protein Ref.1.

Tissue specificity

Highly expressed in the placenta, pancreas, mammary gland and skeletal muscle. Weakly or not expressed in colon, heart and prostate. Ref.1

Sequence similarities

Belongs to the nucleotide-sugar transporter family. SLC35B subfamily.

Biophysicochemical properties

Kinetic parameters:

KM=0.8 µM for PAPS Ref.1

Sequence caution

The sequence CAI20100.2 differs from that shown. Reason: Erroneous gene model prediction.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8TB61-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8TB61-2)

The sequence of this isoform differs from the canonical sequence as follows:
     272-311: Missing.
Isoform 3 (identifier: Q8TB61-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-49: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 432432Adenosine 3'-phospho 5'-phosphosulfate transporter 1
PRO_0000213374

Regions

Transmembrane5 – 2521Helical; Potential
Transmembrane40 – 6021Helical; Potential
Transmembrane109 – 12921Helical; Potential
Transmembrane154 – 17421Helical; Potential
Transmembrane238 – 25821Helical; Potential
Transmembrane265 – 28521Helical; Potential
Transmembrane299 – 31921Helical; Potential
Transmembrane353 – 37321Helical; Potential
Transmembrane387 – 40721Helical; Potential

Amino acid modifications

Modified residue4271Phosphoserine Ref.8 Ref.11

Natural variations

Alternative sequence1 – 4949Missing in isoform 3.
VSP_026949
Alternative sequence272 – 31140Missing in isoform 2.
VSP_014084
Natural variant3421L → V.
Corresponds to variant rs3734707 [ dbSNP | Ensembl ].
VAR_022657

Experimental info

Sequence conflict2141L → P in BAD96748. Ref.7
Sequence conflict3361E → G in BAC11631. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 7E4E9E87231072FB

FASTA43247,515
        10         20         30         40         50         60 
MDARWWAVVV LAAFPSLGAG GETPEAPPES WTQLWFFRFV VNAAGYASFM VPGYLLVQYF 

        70         80         90        100        110        120 
RRKNYLETGR GLCFPLVKAC VFGNEPKASD EVPLAPRTEA AETTPMWQAL KLLFCATGLQ 

       130        140        150        160        170        180 
VSYLTWGVLQ ERVMTRSYGA TATSPGERFT DSQFLVLMNR VLALIVAGLS CVLCKQPRHG 

       190        200        210        220        230        240 
APMYRYSFAS LSNVLSSWCQ YEALKFVSFP TQVLAKASKV IPVMLMGKLV SRRSYEHWEY 

       250        260        270        280        290        300 
LTATLISIGV SMFLLSSGPE PRSSPATTLS GLILLAGYIA FDSFTSNWQD ALFAYKMSSV 

       310        320        330        340        350        360 
QMMFGVNFFS CLFTVGSLLE QGALLEGTRF MGRHSEFAAH ALLLSICSAC GQLFIFYTIG 

       370        380        390        400        410        420 
QFGAAVFTII MTLRQAFAIL LSCLLYGHTV TVVGGLGVAV VFAALLLRVY ARGRLKQRGK 

       430 
KAVPVESPVQ KV 

« Hide

Isoform 2 [UniParc].

Checksum: 9B7BACC7F3F670DD
Show »

FASTA39242,967
Isoform 3 [UniParc].

Checksum: 49A2443E2C37F263
Show »

FASTA38342,128

References

« Hide 'large scale' references
[1]"Molecular cloning and identification of 3'-phosphoadenosine 5'-phosphosulfate transporter."
Kamiyama S., Suda T., Ueda R., Suzuki M., Okubo R., Kikuchi N., Chiba Y., Goto S., Toyoda H., Saigo K., Watanabe M., Narimatsu H., Jigami Y., Nishihara S.
J. Biol. Chem. 278:25958-25963(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Tissue: Colon.
[2]"Identification of a splicing variant of solute carrier family 35, member B2 (SLC35B2)."
Xu J., Xie Y., Mao Y.
Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
[3]"Large-scale identification and characterization of human genes that activate NF-kappaB and MAPK signaling pathways."
Matsuda A., Suzuki Y., Honda G., Muramatsu S., Matsuzaki O., Nagano Y., Doi T., Shimotohno K., Harada T., Nishida E., Hayashi H., Sugano S.
Oncogene 22:3307-3318(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Lung fibroblast.
[4]"Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries."
Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y. expand/collapse author list , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T.
DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Placenta.
[5]"The DNA sequence and analysis of human chromosome 6."
Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Lung carcinoma and Neuroblastoma.
[7]Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 64-432 (ISOFORMS 1/3).
Tissue: Thyroid.
[8]"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-427, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[9]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[10]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-427, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[12]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Web resources

GGDB

GlycoGene database

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB106538 mRNA. Translation: BAC79117.1.
AY491520 mRNA. Translation: AAS79661.1.
AB097021 mRNA. Translation: BAC77374.1.
AB097039 mRNA. Translation: BAC77392.1.
AK075456 mRNA. Translation: BAC11631.1.
AL139392 Genomic DNA. Translation: CAI20100.2. Sequence problems.
AL139392 Genomic DNA. Translation: CAI20101.2.
BC016839 mRNA. Translation: AAH16839.1.
BC024288 mRNA. Translation: AAH24288.1.
AK223028 mRNA. Translation: BAD96748.1.
RefSeqNP_001273438.1. NM_001286509.1.
NP_001273439.1. NM_001286510.1.
NP_001273440.1. NM_001286511.1.
NP_001273441.1. NM_001286512.1.
NP_001273442.1. NM_001286513.1.
NP_001273446.1. NM_001286517.1.
NP_001273448.1. NM_001286519.1.
NP_001273449.1. NM_001286520.1.
NP_835361.1. NM_178148.3.
XP_005249141.1. XM_005249084.1.
UniGeneHs.182885.

3D structure databases

ProteinModelPortalQ8TB61.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid131484. 2 interactions.
STRING9606.ENSP00000377401.

Protein family/group databases

TCDB2.A.7.11.3. the drug/metabolite transporter (dmt) superfamily.

PTM databases

PhosphoSiteQ8TB61.

Polymorphism databases

DMDM67461576.

Proteomic databases

PaxDbQ8TB61.
PRIDEQ8TB61.

Protocols and materials databases

DNASU347734.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000393812; ENSP00000377401; ENSG00000157593. [Q8TB61-1]
GeneID347734.
KEGGhsa:347734.
UCSCuc003oxd.3. human. [Q8TB61-1]

Organism-specific databases

CTD347734.
GeneCardsGC06M044221.
HGNCHGNC:16872. SLC35B2.
HPAHPA029638.
MIM610788. gene.
neXtProtNX_Q8TB61.
PharmGKBPA134927864.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0697.
HOVERGENHBG062347.
InParanoidQ8TB61.
KOK15276.
OMAVLCKQPR.
PhylomeDBQ8TB61.
TreeFamTF105926.

Enzyme and pathway databases

ReactomeREACT_111217. Metabolism.
REACT_116125. Disease.
REACT_15518. Transmembrane transport of small molecules.

Gene expression databases

ArrayExpressQ8TB61.
BgeeQ8TB61.
CleanExHS_SLC35B2.
GenevestigatorQ8TB61.

Family and domain databases

InterProIPR013657. UAA.
[Graphical view]
PfamPF08449. UAA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSLC35B2. human.
GeneWikiSLC35B2.
GenomeRNAi347734.
NextBio99266.
PROQ8TB61.
SOURCESearch...

Entry information

Entry nameS35B2_HUMAN
AccessionPrimary (citable) accession number: Q8TB61
Secondary accession number(s): Q2VY06 expand/collapse secondary AC list , Q53GA3, Q5T9W1, Q5T9W2, Q7Z2G3, Q8NBK6, Q96AR6
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 1, 2002
Last modified: March 19, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM