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Protein

Adenosine 3'-phospho 5'-phosphosulfate transporter 1

Gene

SLC35B2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the transport of adenosine 3'-phospho 5'-phosphosulfate (PAPS), from cytosol into Golgi. PAPS is a universal sulfuryl donor for sulfation events that take place in the Golgi. May indirectly participate in activation of the NF-kappa-B and MAPK pathways.1 Publication

Kineticsi

  1. KM=0.8 µM for PAPS1 Publication

    GO - Molecular functioni

    • 3'-phosphoadenosine 5'-phosphosulfate transmembrane transporter activity Source: UniProtKB
    • signal transducer activity Source: UniProtKB

    GO - Biological processi

    • 3'-phosphoadenosine 5'-phosphosulfate biosynthetic process Source: Reactome
    • 3'-phosphoadenosine 5'-phosphosulfate transport Source: UniProtKB
    • positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB

    Keywordsi

    Biological processTransport

    Enzyme and pathway databases

    ReactomeiR-HSA-174362. Transport and synthesis of PAPS.
    R-HSA-727802. Transport of nucleotide sugars.

    Protein family/group databases

    TCDBi2.A.7.11.3. the drug/metabolite transporter (dmt) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Adenosine 3'-phospho 5'-phosphosulfate transporter 1
    Alternative name(s):
    PAPS transporter 1
    Putative MAPK-activating protein PM15
    Putative NF-kappa-B-activating protein 48
    Solute carrier family 35 member B2
    Gene namesi
    Name:SLC35B2
    Synonyms:PAPST1
    ORF Names:PSEC0149
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 6

    Organism-specific databases

    HGNCiHGNC:16872. SLC35B2.

    Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei5 – 25HelicalSequence analysisAdd BLAST21
    Transmembranei40 – 60HelicalSequence analysisAdd BLAST21
    Transmembranei109 – 129HelicalSequence analysisAdd BLAST21
    Transmembranei154 – 174HelicalSequence analysisAdd BLAST21
    Transmembranei238 – 258HelicalSequence analysisAdd BLAST21
    Transmembranei265 – 285HelicalSequence analysisAdd BLAST21
    Transmembranei299 – 319HelicalSequence analysisAdd BLAST21
    Transmembranei353 – 373HelicalSequence analysisAdd BLAST21
    Transmembranei387 – 407HelicalSequence analysisAdd BLAST21

    GO - Cellular componenti

    • Golgi apparatus Source: UniProtKB
    • Golgi membrane Source: Reactome
    • integral component of endoplasmic reticulum membrane Source: GO_Central
    • integral component of Golgi membrane Source: GO_Central
    • integral component of membrane Source: UniProtKB
    • membrane Source: UniProtKB

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    Pathology & Biotechi

    Organism-specific databases

    DisGeNETi347734.
    OpenTargetsiENSG00000157593.
    PharmGKBiPA134927864.

    Polymorphism and mutation databases

    DMDMi67461576.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002133741 – 432Adenosine 3'-phospho 5'-phosphosulfate transporter 1Add BLAST432

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei427PhosphoserineCombined sources1

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    EPDiQ8TB61.
    MaxQBiQ8TB61.
    PaxDbiQ8TB61.
    PeptideAtlasiQ8TB61.
    PRIDEiQ8TB61.

    PTM databases

    iPTMnetiQ8TB61.
    PhosphoSitePlusiQ8TB61.
    SwissPalmiQ8TB61.

    Expressioni

    Tissue specificityi

    Highly expressed in the placenta, pancreas, mammary gland and skeletal muscle. Weakly or not expressed in colon, heart and prostate.1 Publication

    Gene expression databases

    BgeeiENSG00000157593.
    CleanExiHS_SLC35B2.
    ExpressionAtlasiQ8TB61. baseline and differential.
    GenevisibleiQ8TB61. HS.

    Organism-specific databases

    HPAiHPA029638.

    Interactioni

    Protein-protein interaction databases

    BioGridi131484. 36 interactors.
    IntActiQ8TB61. 13 interactors.
    STRINGi9606.ENSP00000377401.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8TB61.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1581. Eukaryota.
    COG0697. LUCA.
    GeneTreeiENSGT00530000063090.
    HOVERGENiHBG062347.
    KOiK15276.
    OMAiQMMCGVN.
    OrthoDBiEOG091G07O2.
    PhylomeDBiQ8TB61.
    TreeFamiTF105926.

    Family and domain databases

    InterProiView protein in InterPro
    IPR013657. UAA.
    PfamiView protein in Pfam
    PF08449. UAA. 1 hit.

    Sequences (5)i

    Sequence statusi: Complete.

    This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: Q8TB61-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MDARWWAVVV LAAFPSLGAG GETPEAPPES WTQLWFFRFV VNAAGYASFM
    60 70 80 90 100
    VPGYLLVQYF RRKNYLETGR GLCFPLVKAC VFGNEPKASD EVPLAPRTEA
    110 120 130 140 150
    AETTPMWQAL KLLFCATGLQ VSYLTWGVLQ ERVMTRSYGA TATSPGERFT
    160 170 180 190 200
    DSQFLVLMNR VLALIVAGLS CVLCKQPRHG APMYRYSFAS LSNVLSSWCQ
    210 220 230 240 250
    YEALKFVSFP TQVLAKASKV IPVMLMGKLV SRRSYEHWEY LTATLISIGV
    260 270 280 290 300
    SMFLLSSGPE PRSSPATTLS GLILLAGYIA FDSFTSNWQD ALFAYKMSSV
    310 320 330 340 350
    QMMFGVNFFS CLFTVGSLLE QGALLEGTRF MGRHSEFAAH ALLLSICSAC
    360 370 380 390 400
    GQLFIFYTIG QFGAAVFTII MTLRQAFAIL LSCLLYGHTV TVVGGLGVAV
    410 420 430
    VFAALLLRVY ARGRLKQRGK KAVPVESPVQ KV
    Length:432
    Mass (Da):47,515
    Last modified:June 1, 2002 - v1
    Checksum:i7E4E9E87231072FB
    GO
    Isoform 2 (identifier: Q8TB61-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         272-311: Missing.

    Show »
    Length:392
    Mass (Da):42,967
    Checksum:i9B7BACC7F3F670DD
    GO
    Isoform 3 (identifier: Q8TB61-3) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-49: Missing.

    Note: No experimental confirmation available.
    Show »
    Length:383
    Mass (Da):42,128
    Checksum:i49A2443E2C37F263
    GO
    Isoform 4 (identifier: Q8TB61-4) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-27: MDARWWAVVVLAAFPSLGAGGETPEAP → MLLAMPALWYLATSWCSTSGGRTTWRP
         28-120: Missing.

    Note: No experimental confirmation available.
    Show »
    Length:339
    Mass (Da):37,235
    Checksum:iBD3CA2176C9D31C8
    GO
    Isoform 5 (identifier: Q8TB61-5) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-133: Missing.

    Note: No experimental confirmation available.
    Show »
    Length:299
    Mass (Da):32,664
    Checksum:iC2DB27B67918AABB
    GO

    Sequence cautioni

    The sequence CAI20100 differs from that shown. Reason: Erroneous gene model prediction.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti214L → P in BAD96748 (Ref. 8) Curated1
    Sequence conflicti336E → G in BAC11631 (PubMed:16303743).Curated1

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural variantiVAR_022657342L → V1 PublicationCorresponds to variant dbSNP:rs3734707Ensembl.1

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_0574361 – 133Missing in isoform 5. 1 PublicationAdd BLAST133
    Alternative sequenceiVSP_0269491 – 49Missing in isoform 3. 1 PublicationAdd BLAST49
    Alternative sequenceiVSP_0546961 – 27MDARW…TPEAP → MLLAMPALWYLATSWCSTSG GRTTWRP in isoform 4. 1 PublicationAdd BLAST27
    Alternative sequenceiVSP_05469728 – 120Missing in isoform 4. 1 PublicationAdd BLAST93
    Alternative sequenceiVSP_014084272 – 311Missing in isoform 2. 1 PublicationAdd BLAST40

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB106538 mRNA. Translation: BAC79117.1.
    AY491520 mRNA. Translation: AAS79661.1.
    AB097021 mRNA. Translation: BAC77374.1.
    AB097039 mRNA. Translation: BAC77392.1.
    AK293251 mRNA. Translation: BAG56783.1.
    AK293344 mRNA. Translation: BAG56860.1.
    AK075456 mRNA. Translation: BAC11631.1.
    AL139392 Genomic DNA. Translation: CAI20100.2. Sequence problems.
    AL139392 Genomic DNA. Translation: CAI20101.2.
    BC016839 mRNA. Translation: AAH16839.1.
    BC024288 mRNA. Translation: AAH24288.1.
    AK223028 mRNA. Translation: BAD96748.1.
    CCDSiCCDS34462.1. [Q8TB61-1]
    CCDS69127.1. [Q8TB61-4]
    CCDS75462.1. [Q8TB61-5]
    CCDS75463.1. [Q8TB61-3]
    RefSeqiNP_001273438.1. NM_001286509.1.
    NP_001273439.1. NM_001286510.1.
    NP_001273440.1. NM_001286511.1. [Q8TB61-3]
    NP_001273441.1. NM_001286512.1. [Q8TB61-3]
    NP_001273442.1. NM_001286513.1. [Q8TB61-4]
    NP_001273446.1. NM_001286517.1.
    NP_001273448.1. NM_001286519.1. [Q8TB61-5]
    NP_001273449.1. NM_001286520.1. [Q8TB61-5]
    NP_835361.1. NM_178148.3. [Q8TB61-1]
    UniGeneiHs.182885.

    Genome annotation databases

    EnsembliENST00000393812; ENSP00000377401; ENSG00000157593. [Q8TB61-1]
    ENST00000537814; ENSP00000440340; ENSG00000157593. [Q8TB61-5]
    ENST00000538577; ENSP00000443845; ENSG00000157593. [Q8TB61-4]
    ENST00000615337; ENSP00000480681; ENSG00000157593. [Q8TB61-3]
    ENST00000619636; ENSP00000483181; ENSG00000157593. [Q8TB61-3]
    GeneIDi347734.
    KEGGihsa:347734.
    UCSCiuc003oxd.5. human. [Q8TB61-1]
    uc011dvu.4. human.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Similar proteinsi

    Entry informationi

    Entry nameiS35B2_HUMAN
    AccessioniPrimary (citable) accession number: Q8TB61
    Secondary accession number(s): B4DDM2
    , B4DDU9, F5H7Y9, Q2VY06, Q53GA3, Q5T9W1, Q5T9W2, Q7Z2G3, Q8NBK6, Q96AR6
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
    Last sequence update: June 1, 2002
    Last modified: August 30, 2017
    This is version 135 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 6
      Human chromosome 6: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families