Q8TB61 (S35B2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenosine 3'-phospho 5'-phosphosulfate transporter 1

Alternative name(s):
PAPS transporter 1
Putative MAPK-activating protein PM15
Putative NF-kappa-B-activating protein 48
Solute carrier family 35 member B2

Gene names

Name:	SLC35B2
Synonyms:	PAPST1
ORF Names:	PSEC0149

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mediates the transport of adenosine 3'-phospho 5'-phosphosulfate (PAPS), from cytosol into Golgi. PAPS is a universal sulfuryl donor for sulfation events that take place in the Golgi. May indirectly participate in activation of the NF-kappa-B and MAPK pathways. Ref.1
Subcellular location	Golgi apparatus membrane; Multi-pass membrane protein Ref.1.
Tissue specificity	Highly expressed in the placenta, pancreas, mammary gland and skeletal muscle. Weakly or not expressed in colon, heart and prostate. Ref.1
Sequence similarities	Belongs to the nucleotide-sugar transporter family. SLC35B subfamily.
Biophysicochemical properties	Kinetic parameters: KM=0.8 µM for PAPS Ref.1
Sequence caution	The sequence CAI20100.2 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Transport
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	3'-phosphoadenosine 5'-phosphosulfate transport Inferred from direct assay Ref.1. Source: UniProtKB positive regulation of I-kappaB kinase/NF-kappaB cascade Inferred from mutant phenotype Ref.3. Source: UniProtKB
Cellular_component	Golgi membrane Traceable author statement. Source: Reactome integral to membrane Non-traceable author statement Ref.1. Source: UniProtKB
Molecular_function	3'-phosphoadenosine 5'-phosphosulfate transmembrane transporter activity Inferred from direct assay Ref.1. Source: UniProtKB signal transducer activity Inferred from mutant phenotype Ref.3. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8TB61-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8TB61-2)

The sequence of this isoform differs from the canonical sequence as follows:
     272-311: Missing.

Isoform 3 (identifier: Q8TB61-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-49: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 432	432	Adenosine 3'-phospho 5'-phosphosulfate transporter 1		PRO_0000213374
Regions
	Transmembrane	5 – 25	21	Helical; Potential
	Transmembrane	40 – 60	21	Helical; Potential
	Transmembrane	109 – 129	21	Helical; Potential
	Transmembrane	154 – 174	21	Helical; Potential
	Transmembrane	238 – 258	21	Helical; Potential
	Transmembrane	265 – 285	21	Helical; Potential
	Transmembrane	299 – 319	21	Helical; Potential
	Transmembrane	353 – 373	21	Helical; Potential
	Transmembrane	387 – 407	21	Helical; Potential
Amino acid modifications
	Modified residue	427	1	Phosphoserine Ref.8 Ref.10
Natural variations
	Alternative sequence	1 – 49	49	Missing in isoform 3.		VSP_026949
	Alternative sequence	272 – 311	40	Missing in isoform 2.		VSP_014084
	Natural variant	342	1	L → V. Corresponds to variant rs3734707 [ dbSNP | Ensembl ].		VAR_022657
Experimental info
	Sequence conflict	214	1	L → P in BAD96748. Ref.7
	Sequence conflict	336	1	E → G in BAC11631. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 7E4E9E87231072FB	FASTA	432	47,515
	10 20 30 40 50 60 MDARWWAVVV LAAFPSLGAG GETPEAPPES WTQLWFFRFV VNAAGYASFM VPGYLLVQYF 70 80 90 100 110 120 RRKNYLETGR GLCFPLVKAC VFGNEPKASD EVPLAPRTEA AETTPMWQAL KLLFCATGLQ 130 140 150 160 170 180 VSYLTWGVLQ ERVMTRSYGA TATSPGERFT DSQFLVLMNR VLALIVAGLS CVLCKQPRHG 190 200 210 220 230 240 APMYRYSFAS LSNVLSSWCQ YEALKFVSFP TQVLAKASKV IPVMLMGKLV SRRSYEHWEY 250 260 270 280 290 300 LTATLISIGV SMFLLSSGPE PRSSPATTLS GLILLAGYIA FDSFTSNWQD ALFAYKMSSV 310 320 330 340 350 360 QMMFGVNFFS CLFTVGSLLE QGALLEGTRF MGRHSEFAAH ALLLSICSAC GQLFIFYTIG 370 380 390 400 410 420 QFGAAVFTII MTLRQAFAIL LSCLLYGHTV TVVGGLGVAV VFAALLLRVY ARGRLKQRGK 430 KAVPVESPVQ KV
	Isoform 2 [UniParc]. Checksum: 9B7BACC7F3F670DD	FASTA	392	42,967
	10 20 30 40 50 60 MDARWWAVVV LAAFPSLGAG GETPEAPPES WTQLWFFRFV VNAAGYASFM VPGYLLVQYF 70 80 90 100 110 120 RRKNYLETGR GLCFPLVKAC VFGNEPKASD EVPLAPRTEA AETTPMWQAL KLLFCATGLQ 130 140 150 160 170 180 VSYLTWGVLQ ERVMTRSYGA TATSPGERFT DSQFLVLMNR VLALIVAGLS CVLCKQPRHG 190 200 210 220 230 240 APMYRYSFAS LSNVLSSWCQ YEALKFVSFP TQVLAKASKV IPVMLMGKLV SRRSYEHWEY 250 260 270 280 290 300 LTATLISIGV SMFLLSSGPE PRSSPATTLS GLFTVGSLLE QGALLEGTRF MGRHSEFAAH 310 320 330 340 350 360 ALLLSICSAC GQLFIFYTIG QFGAAVFTII MTLRQAFAIL LSCLLYGHTV TVVGGLGVAV 370 380 390 VFAALLLRVY ARGRLKQRGK KAVPVESPVQ KV
	Isoform 3 [UniParc]. Checksum: 49A2443E2C37F263	FASTA	383	42,128
	10 20 30 40 50 60 MVPGYLLVQY FRRKNYLETG RGLCFPLVKA CVFGNEPKAS DEVPLAPRTE AAETTPMWQA 70 80 90 100 110 120 LKLLFCATGL QVSYLTWGVL QERVMTRSYG ATATSPGERF TDSQFLVLMN RVLALIVAGL 130 140 150 160 170 180 SCVLCKQPRH GAPMYRYSFA SLSNVLSSWC QYEALKFVSF PTQVLAKASK VIPVMLMGKL 190 200 210 220 230 240 VSRRSYEHWE YLTATLISIG VSMFLLSSGP EPRSSPATTL SGLILLAGYI AFDSFTSNWQ 250 260 270 280 290 300 DALFAYKMSS VQMMFGVNFF SCLFTVGSLL EQGALLEGTR FMGRHSEFAA HALLLSICSA 310 320 330 340 350 360 CGQLFIFYTI GQFGAAVFTI IMTLRQAFAI LLSCLLYGHT VTVVGGLGVA VVFAALLLRV 370 380 YARGRLKQRG KKAVPVESPV QKV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and identification of 3'-phosphoadenosine 5'-phosphosulfate transporter." Kamiyama S., Suda T., Ueda R., Suzuki M., Okubo R., Kikuchi N., Chiba Y., Goto S., Toyoda H., Saigo K., Watanabe M., Narimatsu H., Jigami Y., Nishihara S. J. Biol. Chem. 278:25958-25963(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Colon.
[2]	"Identification of a splicing variant of solute carrier family 35, member B2 (SLC35B2)." Xu J., Xie Y., Mao Y. Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
[3]	"Large-scale identification and characterization of human genes that activate NF-kappaB and MAPK signaling pathways." Matsuda A., Suzuki Y., Honda G., Muramatsu S., Matsuzaki O., Nagano Y., Doi T., Shimotohno K., Harada T., Nishida E., Hayashi H., Sugano S. Oncogene 22:3307-3318(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Lung fibroblast.
[4]	"Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries." Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y. , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T. DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Placenta.
[5]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Lung carcinoma and Neuroblastoma.
[7]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 64-432 (ISOFORMS 1/3). Tissue: Thyroid.
[8]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-427, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[10]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-427, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Web resources

GGDB

GlycoGene database

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB106538 mRNA. Translation: BAC79117.1. AY491520 mRNA. Translation: AAS79661.1. AB097021 mRNA. Translation: BAC77374.1. AB097039 mRNA. Translation: BAC77392.1. AK075456 mRNA. Translation: BAC11631.1. AL139392 Genomic DNA. Translation: CAI20100.2. Sequence problems. AL139392 Genomic DNA. Translation: CAI20101.2. BC016839 mRNA. Translation: AAH16839.1. BC024288 mRNA. Translation: AAH24288.1. AK223028 mRNA. Translation: BAD96748.1.
IPI	IPI00384867. IPI00642271. IPI00854750.
RefSeq	NP_835361.1. NM_178148.2.
UniGene	Hs.182885.
3D structure databases
ProteinModelPortal	Q8TB61.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000377401.
Protein family/group databases
TCDB	2.A.7.11.3. drug/metabolite transporter (DMT) superfamily.
PTM databases
PhosphoSite	Q8TB61.
Polymorphism databases
DMDM	67461576.
Proteomic databases
PaxDb	Q8TB61.
PRIDE	Q8TB61.
Protocols and materials databases
DNASU	347734.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000393810; ENSP00000377399; ENSG00000157593. ENST00000393812; ENSP00000377401; ENSG00000157593.
GeneID	347734.
KEGG	hsa:347734.
UCSC	uc003oxd.3. human.
Organism-specific databases
CTD	347734.
GeneCards	GC06M044221.
HGNC	HGNC:16872. SLC35B2.
HPA	HPA029638.
MIM	610788. gene.
neXtProt	NX_Q8TB61.
PharmGKB	PA134927864.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0697.
HOVERGEN	HBG062347.
InParanoid	Q8TB61.
KO	K15276.
OMA	YEYYEYV.
PhylomeDB	Q8TB61.
Enzyme and pathway databases
Reactome	REACT_15518. Transmembrane transport of small molecules.
Gene expression databases
ArrayExpress	Q8TB61.
Bgee	Q8TB61.
CleanEx	HS_SLC35B2.
Genevestigator	Q8TB61.
GermOnline	ENSG00000157593. Homo sapiens.
Family and domain databases
InterPro	IPR013657. UAA. [Graphical view]
Pfam	PF08449. UAA. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SLC35B2. human.
GenomeRNAi	347734.
NextBio	99266.
SOURCE	Search...

Entry information

Entry name

S35B2_HUMAN

Accession

Primary (citable) accession number: Q8TB61
Secondary accession number(s): Q2VY06 , Q53GA3, Q5T9W1, Q5T9W2, Q7Z2G3, Q8NBK6, Q96AR6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 1, 2002
Last modified:	April 3, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.