Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q8TB45

- DPTOR_HUMAN

UniProt

Q8TB45 - DPTOR_HUMAN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

DEP domain-containing mTOR-interacting protein

Gene

DEPTOR

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Negative regulator of the mTORC1 and mTORC2 signaling pathways. Inhibits the kinase activity of both complexes.1 Publication

GO - Biological processi

  1. intracellular signal transduction Source: InterPro
  2. negative regulation of cell size Source: UniProtKB
  3. negative regulation of protein kinase activity Source: UniProtKB
  4. negative regulation of TOR signaling Source: UniProtKB
  5. regulation of extrinsic apoptotic signaling pathway Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
DEP domain-containing mTOR-interacting protein
Alternative name(s):
DEP domain-containing protein 6
Gene namesi
Name:DEPTOR
Synonyms:DEPDC6
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 8

Organism-specific databases

HGNCiHGNC:22953. DEPTOR.

Subcellular locationi

GO - Cellular componenti

  1. intracellular Source: LIFEdb
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134897957.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 409409DEP domain-containing mTOR-interacting proteinPRO_0000284784Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine2 Publications
Modified residuei241 – 2411Phosphothreonine1 Publication
Modified residuei244 – 2441Phosphoserine1 Publication
Modified residuei258 – 2581Phosphoserine1 Publication
Modified residuei259 – 2591Phosphothreonine1 Publication
Modified residuei263 – 2631Phosphoserine1 Publication
Modified residuei265 – 2651Phosphoserine1 Publication
Modified residuei282 – 2821Phosphoserine1 Publication
Modified residuei283 – 2831Phosphoserine1 Publication
Modified residuei287 – 2871Phosphoserine1 Publication
Modified residuei293 – 2931Phosphoserine1 Publication
Modified residuei297 – 2971Phosphoserine1 Publication
Modified residuei298 – 2981Phosphoserine1 Publication
Modified residuei299 – 2991Phosphoserine1 Publication

Post-translational modificationi

Phosphorylated. Phosphorylation weakens interaction with MTOR within mTORC1 and mTORC2.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ8TB45.
PaxDbiQ8TB45.
PRIDEiQ8TB45.

PTM databases

PhosphoSiteiQ8TB45.

Expressioni

Inductioni

Expression is negatively regulated by both mTORC1 and mTORC2 (at protein level).1 Publication

Gene expression databases

BgeeiQ8TB45.
CleanExiHS_DEPDC6.
GenevestigatoriQ8TB45.

Organism-specific databases

HPAiCAB020841.
HPA023938.
HPA023945.
HPA024011.

Interactioni

Subunit structurei

Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR, AKT1S1/PRAS40 and DEPTOR. Part of the mammalian target of rapamycin complex 2 (mTORC2) which contains MTOR, MLST8, PROTOR1, RICTOR, MAPKAP1 and DEPTOR. Interacts (via PDZ domain) with MTOR; interacts with MTOR within both mammalian target of rapamycin complexes mTORC1 and mTORC2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
MTORP423455EBI-2359040,EBI-359260
MtorP423462EBI-2359040,EBI-1571489From a different organism.

Protein-protein interaction databases

BioGridi122304. 12 interactions.
IntActiQ8TB45. 7 interactions.
MINTiMINT-4712528.

Structurei

3D structure databases

ProteinModelPortaliQ8TB45.
SMRiQ8TB45. Positions 32-121, 336-404.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini36 – 11984DEP 1PROSITE-ProRule annotationAdd
BLAST
Domaini145 – 21975DEP 2PROSITE-ProRule annotationAdd
BLAST
Domaini330 – 40778PDZPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi222 – 2265Poly-Arg
Compositional biasi235 – 29965Ser-richAdd
BLAST

Sequence similaritiesi

Contains 2 DEP domains.PROSITE-ProRule annotation
Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG125414.
GeneTreeiENSGT00520000055667.
HOVERGENiHBG056008.
InParanoidiQ8TB45.
OMAiRIYEKLM.
PhylomeDBiQ8TB45.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
2.30.42.10. 1 hit.
InterProiIPR000591. DEP_dom.
IPR001478. PDZ.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00610. DEP. 2 hits.
[Graphical view]
SMARTiSM00049. DEP. 2 hits.
SM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
PROSITEiPS50186. DEP. 2 hits.
PS50106. PDZ. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8TB45-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEEGGSTGSA GSDSSTSGSG GAQQRELERM AEVLVTGEQL RLRLHEEKVI
60 70 80 90 100
KDRRHHLKTY PNCFVAKELI DWLIEHKEAS DRETAIKLMQ KLADRGIIHH
110 120 130 140 150
VCDEHKEFKD VKLFYRFRKD DGTFPLDNEV KAFMRGQRLY EKLMSPENTL
160 170 180 190 200
LQPREEEGVK YERTFMASEF LDWLVQEGEA TTRKEAEQLC HRLMEHGIIQ
210 220 230 240 250
HVSNKHPFVD SNLLYQFRMN FRRRRRLMEL LNEKSPSSQE THDSPFCLRK
260 270 280 290 300
QSHDNRKSTS FMSVSPSKEI KIVSAVRRSS MSSCGSSGYF SSSPTLSSSP
310 320 330 340 350
PVLCNPKSVL KRPVTSEELL TPGAPYARKT FTIVGDAVGW GFVVRGSKPC
360 370 380 390 400
HIQAVDPSGP AAAAGMKVCQ FVVSVNGLNV LHVDYRTVSN LILTGPRTIV

MEVMEELEC
Length:409
Mass (Da):46,294
Last modified:July 7, 2009 - v2
Checksum:iAD132F0E9AFFB90C
GO
Isoform 2 (identifier: Q8TB45-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     42-142: Missing.

Note: No experimental confirmation available.

Show »
Length:308
Mass (Da):34,006
Checksum:i257BE95DD14DE19C
GO

Sequence cautioni

The sequence BAB14723.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti166 – 1661M → V in BAB14054. (PubMed:14702039)Curated
Sequence conflicti249 – 2491R → G in CAB66613. (PubMed:11230166)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti148 – 1481N → S.
Corresponds to variant rs34057546 [ dbSNP | Ensembl ].
VAR_031816
Natural varianti204 – 2041N → S.3 Publications
Corresponds to variant rs2271900 [ dbSNP | Ensembl ].
VAR_031817
Natural varianti389 – 3891S → N.2 Publications
Corresponds to variant rs4871827 [ dbSNP | Ensembl ].
VAR_031818

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei42 – 142101Missing in isoform 2. 1 PublicationVSP_054681Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL136678 mRNA. Translation: CAB66613.1.
AK022490 mRNA. Translation: BAB14054.1.
AK023916 mRNA. Translation: BAB14723.1. Different initiation.
AK297822 mRNA. Translation: BAG60159.1.
AK315175 mRNA. Translation: BAG37616.1.
AC091563 Genomic DNA. No translation available.
AP005717 Genomic DNA. No translation available.
CH471060 Genomic DNA. Translation: EAW91997.1.
BC012040 mRNA. Translation: AAH12040.1.
BC024746 mRNA. Translation: AAH24746.1.
CCDSiCCDS6331.1. [Q8TB45-1]
CCDS64960.1. [Q8TB45-2]
RefSeqiNP_001269941.1. NM_001283012.1. [Q8TB45-2]
NP_073620.2. NM_022783.3. [Q8TB45-1]
UniGeneiHs.112981.

Genome annotation databases

EnsembliENST00000286234; ENSP00000286234; ENSG00000155792. [Q8TB45-1]
ENST00000523492; ENSP00000430457; ENSG00000155792. [Q8TB45-2]
GeneIDi64798.
KEGGihsa:64798.
UCSCiuc003yow.4. human. [Q8TB45-1]

Polymorphism databases

DMDMi251757257.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL136678 mRNA. Translation: CAB66613.1 .
AK022490 mRNA. Translation: BAB14054.1 .
AK023916 mRNA. Translation: BAB14723.1 . Different initiation.
AK297822 mRNA. Translation: BAG60159.1 .
AK315175 mRNA. Translation: BAG37616.1 .
AC091563 Genomic DNA. No translation available.
AP005717 Genomic DNA. No translation available.
CH471060 Genomic DNA. Translation: EAW91997.1 .
BC012040 mRNA. Translation: AAH12040.1 .
BC024746 mRNA. Translation: AAH24746.1 .
CCDSi CCDS6331.1. [Q8TB45-1 ]
CCDS64960.1. [Q8TB45-2 ]
RefSeqi NP_001269941.1. NM_001283012.1. [Q8TB45-2 ]
NP_073620.2. NM_022783.3. [Q8TB45-1 ]
UniGenei Hs.112981.

3D structure databases

ProteinModelPortali Q8TB45.
SMRi Q8TB45. Positions 32-121, 336-404.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 122304. 12 interactions.
IntActi Q8TB45. 7 interactions.
MINTi MINT-4712528.

PTM databases

PhosphoSitei Q8TB45.

Polymorphism databases

DMDMi 251757257.

Proteomic databases

MaxQBi Q8TB45.
PaxDbi Q8TB45.
PRIDEi Q8TB45.

Protocols and materials databases

DNASUi 64798.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000286234 ; ENSP00000286234 ; ENSG00000155792 . [Q8TB45-1 ]
ENST00000523492 ; ENSP00000430457 ; ENSG00000155792 . [Q8TB45-2 ]
GeneIDi 64798.
KEGGi hsa:64798.
UCSCi uc003yow.4. human. [Q8TB45-1 ]

Organism-specific databases

CTDi 64798.
GeneCardsi GC08P120886.
H-InvDB HIX0017353.
HIX0025586.
HGNCi HGNC:22953. DEPTOR.
HPAi CAB020841.
HPA023938.
HPA023945.
HPA024011.
MIMi 612974. gene.
neXtProti NX_Q8TB45.
PharmGKBi PA134897957.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG125414.
GeneTreei ENSGT00520000055667.
HOVERGENi HBG056008.
InParanoidi Q8TB45.
OMAi RIYEKLM.
PhylomeDBi Q8TB45.

Miscellaneous databases

GeneWikii DEPTOR.
GenomeRNAii 64798.
NextBioi 35473550.
PROi Q8TB45.
SOURCEi Search...

Gene expression databases

Bgeei Q8TB45.
CleanExi HS_DEPDC6.
Genevestigatori Q8TB45.

Family and domain databases

Gene3Di 1.10.10.10. 2 hits.
2.30.42.10. 1 hit.
InterProi IPR000591. DEP_dom.
IPR001478. PDZ.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF00610. DEP. 2 hits.
[Graphical view ]
SMARTi SM00049. DEP. 2 hits.
SM00228. PDZ. 1 hit.
[Graphical view ]
SUPFAMi SSF50156. SSF50156. 1 hit.
PROSITEi PS50186. DEP. 2 hits.
PS50106. PDZ. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT SER-204.
    Tissue: Brain.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANTS SER-204 AND ASN-389.
    Tissue: Heart, Mammary gland and Thyroid.
  3. "DNA sequence and analysis of human chromosome 8."
    Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T.
    , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
    Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS SER-204 AND ASN-389.
    Tissue: Cervix and Colon.
  6. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "DEPTOR is an mTOR inhibitor frequently overexpressed in multiple myeloma cells and required for their survival."
    Peterson T.R., Laplante M., Thoreen C.C., Sancak Y., Kang S.A., Kuehl W.M., Gray N.S., Sabatini D.M.
    Cell 137:873-886(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MTOR, INDUCTION, PHOSPHORYLATION AT THR-241; SER-244; SER-258; THR-259; SER-263; SER-265; SER-282; SER-283; SER-287; SER-293; SER-297; SER-298 AND SER-299, IDENTIFICATION BY MASS SPECTROMETRY.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiDPTOR_HUMAN
AccessioniPrimary (citable) accession number: Q8TB45
Secondary accession number(s): B2RCL9
, B4DN97, E7EV87, Q96EQ1, Q9H0R7, Q9H894, Q9HA07
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: July 7, 2009
Last modified: October 29, 2014
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3