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Q8TB45

- DPTOR_HUMAN

UniProt

Q8TB45 - DPTOR_HUMAN

Protein

DEP domain-containing mTOR-interacting protein

Gene

DEPTOR

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 2 (07 Jul 2009)
      Previous versions | rss
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    Functioni

    Negative regulator of the mTORC1 and mTORC2 signaling pathways. Inhibits the kinase activity of both complexes.1 Publication

    GO - Molecular functioni

    1. protein binding Source: UniProtKB

    GO - Biological processi

    1. intracellular signal transduction Source: InterPro
    2. negative regulation of cell size Source: UniProtKB
    3. negative regulation of protein kinase activity Source: UniProtKB
    4. negative regulation of TOR signaling Source: UniProtKB
    5. regulation of extrinsic apoptotic signaling pathway Source: UniProtKB

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DEP domain-containing mTOR-interacting protein
    Alternative name(s):
    DEP domain-containing protein 6
    Gene namesi
    Name:DEPTOR
    Synonyms:DEPDC6
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 8

    Organism-specific databases

    HGNCiHGNC:22953. DEPTOR.

    Subcellular locationi

    GO - Cellular componenti

    1. intracellular Source: LIFEdb

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134897957.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 409409DEP domain-containing mTOR-interacting proteinPRO_0000284784Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine2 Publications
    Modified residuei241 – 2411Phosphothreonine1 Publication
    Modified residuei244 – 2441Phosphoserine1 Publication
    Modified residuei258 – 2581Phosphoserine1 Publication
    Modified residuei259 – 2591Phosphothreonine1 Publication
    Modified residuei263 – 2631Phosphoserine1 Publication
    Modified residuei265 – 2651Phosphoserine1 Publication
    Modified residuei282 – 2821Phosphoserine1 Publication
    Modified residuei283 – 2831Phosphoserine1 Publication
    Modified residuei287 – 2871Phosphoserine1 Publication
    Modified residuei293 – 2931Phosphoserine1 Publication
    Modified residuei297 – 2971Phosphoserine1 Publication
    Modified residuei298 – 2981Phosphoserine1 Publication
    Modified residuei299 – 2991Phosphoserine1 Publication

    Post-translational modificationi

    Phosphorylated. Phosphorylation weakens interaction with MTOR within mTORC1 and mTORC2.1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ8TB45.
    PaxDbiQ8TB45.
    PRIDEiQ8TB45.

    PTM databases

    PhosphoSiteiQ8TB45.

    Expressioni

    Inductioni

    Expression is negatively regulated by both mTORC1 and mTORC2 (at protein level).1 Publication

    Gene expression databases

    ArrayExpressiQ8TB45.
    BgeeiQ8TB45.
    CleanExiHS_DEPDC6.
    GenevestigatoriQ8TB45.

    Organism-specific databases

    HPAiCAB020841.
    HPA023938.
    HPA023945.
    HPA024011.

    Interactioni

    Subunit structurei

    Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR, AKT1S1/PRAS40 and DEPTOR. Part of the mammalian target of rapamycin complex 2 (mTORC2) which contains MTOR, MLST8, PROTOR1, RICTOR, MAPKAP1 and DEPTOR. Interacts (via PDZ domain) with MTOR; interacts with MTOR within both mammalian target of rapamycin complexes mTORC1 and mTORC2.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MTORP423455EBI-2359040,EBI-359260
    MtorP423462EBI-2359040,EBI-1571489From a different organism.

    Protein-protein interaction databases

    BioGridi122304. 12 interactions.
    IntActiQ8TB45. 7 interactions.
    MINTiMINT-4712528.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8TB45.
    SMRiQ8TB45. Positions 32-121, 336-404.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini36 – 11984DEP 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini145 – 21975DEP 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini330 – 40778PDZPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi222 – 2265Poly-Arg
    Compositional biasi235 – 29965Ser-richAdd
    BLAST

    Sequence similaritiesi

    Contains 2 DEP domains.PROSITE-ProRule annotation
    Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG125414.
    HOVERGENiHBG056008.
    InParanoidiQ8TB45.
    OMAiRIYEKLM.
    PhylomeDBiQ8TB45.

    Family and domain databases

    Gene3Di1.10.10.10. 2 hits.
    2.30.42.10. 1 hit.
    InterProiIPR000591. DEP_dom.
    IPR001478. PDZ.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00610. DEP. 2 hits.
    [Graphical view]
    SMARTiSM00049. DEP. 2 hits.
    SM00228. PDZ. 1 hit.
    [Graphical view]
    SUPFAMiSSF50156. SSF50156. 1 hit.
    PROSITEiPS50186. DEP. 2 hits.
    PS50106. PDZ. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8TB45-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEEGGSTGSA GSDSSTSGSG GAQQRELERM AEVLVTGEQL RLRLHEEKVI    50
    KDRRHHLKTY PNCFVAKELI DWLIEHKEAS DRETAIKLMQ KLADRGIIHH 100
    VCDEHKEFKD VKLFYRFRKD DGTFPLDNEV KAFMRGQRLY EKLMSPENTL 150
    LQPREEEGVK YERTFMASEF LDWLVQEGEA TTRKEAEQLC HRLMEHGIIQ 200
    HVSNKHPFVD SNLLYQFRMN FRRRRRLMEL LNEKSPSSQE THDSPFCLRK 250
    QSHDNRKSTS FMSVSPSKEI KIVSAVRRSS MSSCGSSGYF SSSPTLSSSP 300
    PVLCNPKSVL KRPVTSEELL TPGAPYARKT FTIVGDAVGW GFVVRGSKPC 350
    HIQAVDPSGP AAAAGMKVCQ FVVSVNGLNV LHVDYRTVSN LILTGPRTIV 400
    MEVMEELEC 409
    Length:409
    Mass (Da):46,294
    Last modified:July 7, 2009 - v2
    Checksum:iAD132F0E9AFFB90C
    GO
    Isoform 2 (identifier: Q8TB45-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         42-142: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:308
    Mass (Da):34,006
    Checksum:i257BE95DD14DE19C
    GO

    Sequence cautioni

    The sequence BAB14723.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti166 – 1661M → V in BAB14054. (PubMed:14702039)Curated
    Sequence conflicti249 – 2491R → G in CAB66613. (PubMed:11230166)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti148 – 1481N → S.
    Corresponds to variant rs34057546 [ dbSNP | Ensembl ].
    VAR_031816
    Natural varianti204 – 2041N → S.3 Publications
    Corresponds to variant rs2271900 [ dbSNP | Ensembl ].
    VAR_031817
    Natural varianti389 – 3891S → N.2 Publications
    Corresponds to variant rs4871827 [ dbSNP | Ensembl ].
    VAR_031818

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei42 – 142101Missing in isoform 2. 1 PublicationVSP_054681Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL136678 mRNA. Translation: CAB66613.1.
    AK022490 mRNA. Translation: BAB14054.1.
    AK023916 mRNA. Translation: BAB14723.1. Different initiation.
    AK297822 mRNA. Translation: BAG60159.1.
    AK315175 mRNA. Translation: BAG37616.1.
    AC091563 Genomic DNA. No translation available.
    AP005717 Genomic DNA. No translation available.
    CH471060 Genomic DNA. Translation: EAW91997.1.
    BC012040 mRNA. Translation: AAH12040.1.
    BC024746 mRNA. Translation: AAH24746.1.
    CCDSiCCDS6331.1. [Q8TB45-1]
    CCDS64960.1. [Q8TB45-2]
    RefSeqiNP_001269941.1. NM_001283012.1. [Q8TB45-2]
    NP_073620.2. NM_022783.3. [Q8TB45-1]
    UniGeneiHs.112981.

    Genome annotation databases

    EnsembliENST00000286234; ENSP00000286234; ENSG00000155792. [Q8TB45-1]
    ENST00000523492; ENSP00000430457; ENSG00000155792. [Q8TB45-2]
    GeneIDi64798.
    KEGGihsa:64798.
    UCSCiuc003yow.4. human. [Q8TB45-1]

    Polymorphism databases

    DMDMi251757257.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL136678 mRNA. Translation: CAB66613.1 .
    AK022490 mRNA. Translation: BAB14054.1 .
    AK023916 mRNA. Translation: BAB14723.1 . Different initiation.
    AK297822 mRNA. Translation: BAG60159.1 .
    AK315175 mRNA. Translation: BAG37616.1 .
    AC091563 Genomic DNA. No translation available.
    AP005717 Genomic DNA. No translation available.
    CH471060 Genomic DNA. Translation: EAW91997.1 .
    BC012040 mRNA. Translation: AAH12040.1 .
    BC024746 mRNA. Translation: AAH24746.1 .
    CCDSi CCDS6331.1. [Q8TB45-1 ]
    CCDS64960.1. [Q8TB45-2 ]
    RefSeqi NP_001269941.1. NM_001283012.1. [Q8TB45-2 ]
    NP_073620.2. NM_022783.3. [Q8TB45-1 ]
    UniGenei Hs.112981.

    3D structure databases

    ProteinModelPortali Q8TB45.
    SMRi Q8TB45. Positions 32-121, 336-404.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 122304. 12 interactions.
    IntActi Q8TB45. 7 interactions.
    MINTi MINT-4712528.

    PTM databases

    PhosphoSitei Q8TB45.

    Polymorphism databases

    DMDMi 251757257.

    Proteomic databases

    MaxQBi Q8TB45.
    PaxDbi Q8TB45.
    PRIDEi Q8TB45.

    Protocols and materials databases

    DNASUi 64798.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000286234 ; ENSP00000286234 ; ENSG00000155792 . [Q8TB45-1 ]
    ENST00000523492 ; ENSP00000430457 ; ENSG00000155792 . [Q8TB45-2 ]
    GeneIDi 64798.
    KEGGi hsa:64798.
    UCSCi uc003yow.4. human. [Q8TB45-1 ]

    Organism-specific databases

    CTDi 64798.
    GeneCardsi GC08P120886.
    H-InvDB HIX0017353.
    HIX0025586.
    HGNCi HGNC:22953. DEPTOR.
    HPAi CAB020841.
    HPA023938.
    HPA023945.
    HPA024011.
    MIMi 612974. gene.
    neXtProti NX_Q8TB45.
    PharmGKBi PA134897957.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG125414.
    HOVERGENi HBG056008.
    InParanoidi Q8TB45.
    OMAi RIYEKLM.
    PhylomeDBi Q8TB45.

    Miscellaneous databases

    GeneWikii DEPTOR.
    GenomeRNAii 64798.
    NextBioi 35473550.
    PROi Q8TB45.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8TB45.
    Bgeei Q8TB45.
    CleanExi HS_DEPDC6.
    Genevestigatori Q8TB45.

    Family and domain databases

    Gene3Di 1.10.10.10. 2 hits.
    2.30.42.10. 1 hit.
    InterProi IPR000591. DEP_dom.
    IPR001478. PDZ.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00610. DEP. 2 hits.
    [Graphical view ]
    SMARTi SM00049. DEP. 2 hits.
    SM00228. PDZ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50156. SSF50156. 1 hit.
    PROSITEi PS50186. DEP. 2 hits.
    PS50106. PDZ. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT SER-204.
      Tissue: Brain.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANTS SER-204 AND ASN-389.
      Tissue: Heart, Mammary gland and Thyroid.
    3. "DNA sequence and analysis of human chromosome 8."
      Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T.
      , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
      Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS SER-204 AND ASN-389.
      Tissue: Cervix and Colon.
    6. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "DEPTOR is an mTOR inhibitor frequently overexpressed in multiple myeloma cells and required for their survival."
      Peterson T.R., Laplante M., Thoreen C.C., Sancak Y., Kang S.A., Kuehl W.M., Gray N.S., Sabatini D.M.
      Cell 137:873-886(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH MTOR, INDUCTION, PHOSPHORYLATION AT THR-241; SER-244; SER-258; THR-259; SER-263; SER-265; SER-282; SER-283; SER-287; SER-293; SER-297; SER-298 AND SER-299, IDENTIFICATION BY MASS SPECTROMETRY.
    8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiDPTOR_HUMAN
    AccessioniPrimary (citable) accession number: Q8TB45
    Secondary accession number(s): B2RCL9
    , B4DN97, E7EV87, Q96EQ1, Q9H0R7, Q9H894, Q9HA07
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 17, 2007
    Last sequence update: July 7, 2009
    Last modified: October 1, 2014
    This is version 96 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 8
      Human chromosome 8: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3