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Protein

DEP domain-containing mTOR-interacting protein

Gene

DEPTOR

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Negative regulator of the mTORC1 and mTORC2 signaling pathways. Inhibits the kinase activity of both complexes.1 Publication

GO - Biological processi

  • intracellular signal transduction Source: InterPro
  • negative regulation of cell size Source: UniProtKB
  • negative regulation of protein kinase activity Source: UniProtKB
  • negative regulation of TOR signaling Source: UniProtKB
  • regulation of extrinsic apoptotic signaling pathway Source: UniProtKB
Complete GO annotation...

Enzyme and pathway databases

BioCyciZFISH:ENSG00000155792-MONOMER.
SIGNORiQ8TB45.

Names & Taxonomyi

Protein namesi
Recommended name:
DEP domain-containing mTOR-interacting protein
Alternative name(s):
DEP domain-containing protein 6
Gene namesi
Name:DEPTOR
Synonyms:DEPDC6
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:22953. DEPTOR.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi64798.
OpenTargetsiENSG00000155792.
PharmGKBiPA134897957.

Polymorphism and mutation databases

BioMutaiDEPTOR.
DMDMi251757257.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002847841 – 409DEP domain-containing mTOR-interacting proteinAdd BLAST409

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei241Phosphothreonine1 Publication1
Modified residuei244PhosphoserineCombined sources1 Publication1
Modified residuei258Phosphoserine1 Publication1
Modified residuei259Phosphothreonine1 Publication1
Modified residuei263Phosphoserine1 Publication1
Modified residuei265Phosphoserine1 Publication1
Modified residuei280PhosphoserineBy similarity1
Modified residuei282Phosphoserine1 Publication1
Modified residuei283Phosphoserine1 Publication1
Modified residuei287Phosphoserine1 Publication1
Modified residuei293Phosphoserine1 Publication1
Modified residuei297Phosphoserine1 Publication1
Modified residuei298Phosphoserine1 Publication1
Modified residuei299Phosphoserine1 Publication1

Post-translational modificationi

Phosphorylated. Phosphorylation weakens interaction with MTOR within mTORC1 and mTORC2.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8TB45.
MaxQBiQ8TB45.
PaxDbiQ8TB45.
PeptideAtlasiQ8TB45.
PRIDEiQ8TB45.

PTM databases

iPTMnetiQ8TB45.
PhosphoSitePlusiQ8TB45.

Expressioni

Inductioni

Expression is negatively regulated by both mTORC1 and mTORC2 (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000155792.
CleanExiHS_DEPDC6.
GenevisibleiQ8TB45. HS.

Organism-specific databases

HPAiCAB020841.
HPA023938.
HPA023945.
HPA024011.

Interactioni

Subunit structurei

Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR, AKT1S1/PRAS40 and DEPTOR. Part of the mammalian target of rapamycin complex 2 (mTORC2) which contains MTOR, MLST8, PROTOR1, RICTOR, MAPKAP1 and DEPTOR. Interacts (via PDZ domain) with MTOR; interacts with MTOR within both mammalian target of rapamycin complexes mTORC1 and mTORC2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CCDC110Q8TBZ03EBI-2359040,EBI-2837012
MTORP423455EBI-2359040,EBI-359260
MtorP423462EBI-2359040,EBI-1571489From a different organism.
TRAF1Q130773EBI-2359040,EBI-359224

Protein-protein interaction databases

BioGridi122304. 21 interactors.
IntActiQ8TB45. 10 interactors.
MINTiMINT-4712528.
STRINGi9606.ENSP00000286234.

Structurei

3D structure databases

ProteinModelPortaliQ8TB45.
SMRiQ8TB45.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini36 – 119DEP 1PROSITE-ProRule annotationAdd BLAST84
Domaini145 – 219DEP 2PROSITE-ProRule annotationAdd BLAST75
Domaini330 – 407PDZPROSITE-ProRule annotationAdd BLAST78

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi222 – 226Poly-Arg5
Compositional biasi235 – 299Ser-richAdd BLAST65

Sequence similaritiesi

Contains 2 DEP domains.PROSITE-ProRule annotation
Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IKSC. Eukaryota.
ENOG4110T1J. LUCA.
GeneTreeiENSGT00520000055667.
HOGENOMiHOG000294178.
HOVERGENiHBG056008.
InParanoidiQ8TB45.
KOiK20402.
OMAiRIYEKLM.
OrthoDBiEOG091G0AB3.
PhylomeDBiQ8TB45.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
2.30.42.10. 1 hit.
InterProiIPR000591. DEP_dom.
IPR001478. PDZ.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00610. DEP. 2 hits.
[Graphical view]
SMARTiSM00049. DEP. 2 hits.
SM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 2 hits.
SSF50156. SSF50156. 1 hit.
PROSITEiPS50186. DEP. 2 hits.
PS50106. PDZ. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8TB45-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEEGGSTGSA GSDSSTSGSG GAQQRELERM AEVLVTGEQL RLRLHEEKVI
60 70 80 90 100
KDRRHHLKTY PNCFVAKELI DWLIEHKEAS DRETAIKLMQ KLADRGIIHH
110 120 130 140 150
VCDEHKEFKD VKLFYRFRKD DGTFPLDNEV KAFMRGQRLY EKLMSPENTL
160 170 180 190 200
LQPREEEGVK YERTFMASEF LDWLVQEGEA TTRKEAEQLC HRLMEHGIIQ
210 220 230 240 250
HVSNKHPFVD SNLLYQFRMN FRRRRRLMEL LNEKSPSSQE THDSPFCLRK
260 270 280 290 300
QSHDNRKSTS FMSVSPSKEI KIVSAVRRSS MSSCGSSGYF SSSPTLSSSP
310 320 330 340 350
PVLCNPKSVL KRPVTSEELL TPGAPYARKT FTIVGDAVGW GFVVRGSKPC
360 370 380 390 400
HIQAVDPSGP AAAAGMKVCQ FVVSVNGLNV LHVDYRTVSN LILTGPRTIV

MEVMEELEC
Length:409
Mass (Da):46,294
Last modified:July 7, 2009 - v2
Checksum:iAD132F0E9AFFB90C
GO
Isoform 2 (identifier: Q8TB45-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     42-142: Missing.

Note: No experimental confirmation available.
Show »
Length:308
Mass (Da):34,006
Checksum:i257BE95DD14DE19C
GO

Sequence cautioni

The sequence BAB14723 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti166M → V in BAB14054 (PubMed:14702039).Curated1
Sequence conflicti249R → G in CAB66613 (PubMed:11230166).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_031816148N → S.Corresponds to variant rs34057546dbSNPEnsembl.1
Natural variantiVAR_031817204N → S.3 PublicationsCorresponds to variant rs2271900dbSNPEnsembl.1
Natural variantiVAR_031818389S → N.2 PublicationsCorresponds to variant rs4871827dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05468142 – 142Missing in isoform 2. 1 PublicationAdd BLAST101

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL136678 mRNA. Translation: CAB66613.1.
AK022490 mRNA. Translation: BAB14054.1.
AK023916 mRNA. Translation: BAB14723.1. Different initiation.
AK297822 mRNA. Translation: BAG60159.1.
AK315175 mRNA. Translation: BAG37616.1.
AC091563 Genomic DNA. No translation available.
AP005717 Genomic DNA. No translation available.
CH471060 Genomic DNA. Translation: EAW91997.1.
BC012040 mRNA. Translation: AAH12040.1.
BC024746 mRNA. Translation: AAH24746.1.
CCDSiCCDS6331.1. [Q8TB45-1]
CCDS64960.1. [Q8TB45-2]
RefSeqiNP_001269941.1. NM_001283012.1. [Q8TB45-2]
NP_073620.2. NM_022783.3. [Q8TB45-1]
UniGeneiHs.112981.

Genome annotation databases

EnsembliENST00000286234; ENSP00000286234; ENSG00000155792. [Q8TB45-1]
ENST00000523492; ENSP00000430457; ENSG00000155792. [Q8TB45-2]
GeneIDi64798.
KEGGihsa:64798.
UCSCiuc003yow.6. human. [Q8TB45-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL136678 mRNA. Translation: CAB66613.1.
AK022490 mRNA. Translation: BAB14054.1.
AK023916 mRNA. Translation: BAB14723.1. Different initiation.
AK297822 mRNA. Translation: BAG60159.1.
AK315175 mRNA. Translation: BAG37616.1.
AC091563 Genomic DNA. No translation available.
AP005717 Genomic DNA. No translation available.
CH471060 Genomic DNA. Translation: EAW91997.1.
BC012040 mRNA. Translation: AAH12040.1.
BC024746 mRNA. Translation: AAH24746.1.
CCDSiCCDS6331.1. [Q8TB45-1]
CCDS64960.1. [Q8TB45-2]
RefSeqiNP_001269941.1. NM_001283012.1. [Q8TB45-2]
NP_073620.2. NM_022783.3. [Q8TB45-1]
UniGeneiHs.112981.

3D structure databases

ProteinModelPortaliQ8TB45.
SMRiQ8TB45.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122304. 21 interactors.
IntActiQ8TB45. 10 interactors.
MINTiMINT-4712528.
STRINGi9606.ENSP00000286234.

PTM databases

iPTMnetiQ8TB45.
PhosphoSitePlusiQ8TB45.

Polymorphism and mutation databases

BioMutaiDEPTOR.
DMDMi251757257.

Proteomic databases

EPDiQ8TB45.
MaxQBiQ8TB45.
PaxDbiQ8TB45.
PeptideAtlasiQ8TB45.
PRIDEiQ8TB45.

Protocols and materials databases

DNASUi64798.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000286234; ENSP00000286234; ENSG00000155792. [Q8TB45-1]
ENST00000523492; ENSP00000430457; ENSG00000155792. [Q8TB45-2]
GeneIDi64798.
KEGGihsa:64798.
UCSCiuc003yow.6. human. [Q8TB45-1]

Organism-specific databases

CTDi64798.
DisGeNETi64798.
GeneCardsiDEPTOR.
H-InvDBHIX0017353.
HIX0025586.
HGNCiHGNC:22953. DEPTOR.
HPAiCAB020841.
HPA023938.
HPA023945.
HPA024011.
MIMi612974. gene.
neXtProtiNX_Q8TB45.
OpenTargetsiENSG00000155792.
PharmGKBiPA134897957.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IKSC. Eukaryota.
ENOG4110T1J. LUCA.
GeneTreeiENSGT00520000055667.
HOGENOMiHOG000294178.
HOVERGENiHBG056008.
InParanoidiQ8TB45.
KOiK20402.
OMAiRIYEKLM.
OrthoDBiEOG091G0AB3.
PhylomeDBiQ8TB45.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000155792-MONOMER.
SIGNORiQ8TB45.

Miscellaneous databases

ChiTaRSiDEPTOR. human.
GeneWikiiDEPTOR.
GenomeRNAii64798.
PROiQ8TB45.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000155792.
CleanExiHS_DEPDC6.
GenevisibleiQ8TB45. HS.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
2.30.42.10. 1 hit.
InterProiIPR000591. DEP_dom.
IPR001478. PDZ.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00610. DEP. 2 hits.
[Graphical view]
SMARTiSM00049. DEP. 2 hits.
SM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 2 hits.
SSF50156. SSF50156. 1 hit.
PROSITEiPS50186. DEP. 2 hits.
PS50106. PDZ. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDPTOR_HUMAN
AccessioniPrimary (citable) accession number: Q8TB45
Secondary accession number(s): B2RCL9
, B4DN97, E7EV87, Q96EQ1, Q9H0R7, Q9H894, Q9HA07
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: July 7, 2009
Last modified: November 2, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.