Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8TB45 (DPTOR_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DEP domain-containing mTOR-interacting protein
Alternative name(s):
DEP domain-containing protein 6
Gene names
Name:DEPTOR
Synonyms:DEPDC6
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length409 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Negative regulator of the mTORC1 and mTORC2 signaling pathways. Inhibits the kinase activity of both complexes. Ref.7

Subunit structure

Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR, AKT1S1/PRAS40 and DEPTOR. Part of the mammalian target of rapamycin complex 2 (mTORC2) which contains MTOR, MLST8, PROTOR1, RICTOR, MAPKAP1 and DEPTOR. Interacts (via PDZ domain) with MTOR; interacts with MTOR within both mammalian target of rapamycin complexes mTORC1 and mTORC2. Ref.7

Induction

Expression is negatively regulated by both mTORC1 and mTORC2 (at protein level). Ref.7

Post-translational modification

Phosphorylated. Phosphorylation weakens interaction with MTOR within mTORC1 and mTORC2. Ref.7

Sequence similarities

Contains 2 DEP domains.

Contains 1 PDZ (DHR) domain.

Sequence caution

The sequence BAB14723.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

MTORP423455EBI-2359040,EBI-359260
MtorP423462EBI-2359040,EBI-1571489From a different organism.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8TB45-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8TB45-2)

The sequence of this isoform differs from the canonical sequence as follows:
     42-142: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 409409DEP domain-containing mTOR-interacting protein
PRO_0000284784

Regions

Domain36 – 11984DEP 1
Domain145 – 21975DEP 2
Domain330 – 40778PDZ
Compositional bias222 – 2265Poly-Arg
Compositional bias235 – 29965Ser-rich

Amino acid modifications

Modified residue11N-acetylmethionine Ref.6 Ref.9
Modified residue2411Phosphothreonine Ref.7
Modified residue2441Phosphoserine Ref.7
Modified residue2581Phosphoserine Ref.7
Modified residue2591Phosphothreonine Ref.7
Modified residue2631Phosphoserine Ref.7
Modified residue2651Phosphoserine Ref.7
Modified residue2821Phosphoserine Ref.7
Modified residue2831Phosphoserine Ref.7
Modified residue2871Phosphoserine Ref.7
Modified residue2931Phosphoserine Ref.7
Modified residue2971Phosphoserine Ref.7
Modified residue2981Phosphoserine Ref.7
Modified residue2991Phosphoserine Ref.7

Natural variations

Alternative sequence42 – 142101Missing in isoform 2.
VSP_054681
Natural variant1481N → S.
Corresponds to variant rs34057546 [ dbSNP | Ensembl ].
VAR_031816
Natural variant2041N → S. Ref.1 Ref.2 Ref.5
Corresponds to variant rs2271900 [ dbSNP | Ensembl ].
VAR_031817
Natural variant3891S → N. Ref.2 Ref.5
Corresponds to variant rs4871827 [ dbSNP | Ensembl ].
VAR_031818

Experimental info

Sequence conflict1661M → V in BAB14054. Ref.2
Sequence conflict2491R → G in CAB66613. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 7, 2009. Version 2.
Checksum: AD132F0E9AFFB90C

FASTA40946,294
        10         20         30         40         50         60 
MEEGGSTGSA GSDSSTSGSG GAQQRELERM AEVLVTGEQL RLRLHEEKVI KDRRHHLKTY 

        70         80         90        100        110        120 
PNCFVAKELI DWLIEHKEAS DRETAIKLMQ KLADRGIIHH VCDEHKEFKD VKLFYRFRKD 

       130        140        150        160        170        180 
DGTFPLDNEV KAFMRGQRLY EKLMSPENTL LQPREEEGVK YERTFMASEF LDWLVQEGEA 

       190        200        210        220        230        240 
TTRKEAEQLC HRLMEHGIIQ HVSNKHPFVD SNLLYQFRMN FRRRRRLMEL LNEKSPSSQE 

       250        260        270        280        290        300 
THDSPFCLRK QSHDNRKSTS FMSVSPSKEI KIVSAVRRSS MSSCGSSGYF SSSPTLSSSP 

       310        320        330        340        350        360 
PVLCNPKSVL KRPVTSEELL TPGAPYARKT FTIVGDAVGW GFVVRGSKPC HIQAVDPSGP 

       370        380        390        400 
AAAAGMKVCQ FVVSVNGLNV LHVDYRTVSN LILTGPRTIV MEVMEELEC 

« Hide

Isoform 2 [UniParc].

Checksum: 257BE95DD14DE19C
Show »

FASTA30834,006

References

« Hide 'large scale' references
[1]"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs."
Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. expand/collapse author list , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A.
Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT SER-204.
Tissue: Brain.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANTS SER-204 AND ASN-389.
Tissue: Heart, Mammary gland and Thyroid.
[3]"DNA sequence and analysis of human chromosome 8."
Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. expand/collapse author list , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS SER-204 AND ASN-389.
Tissue: Cervix and Colon.
[6]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"DEPTOR is an mTOR inhibitor frequently overexpressed in multiple myeloma cells and required for their survival."
Peterson T.R., Laplante M., Thoreen C.C., Sancak Y., Kang S.A., Kuehl W.M., Gray N.S., Sabatini D.M.
Cell 137:873-886(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH MTOR, INDUCTION, PHOSPHORYLATION AT THR-241; SER-244; SER-258; THR-259; SER-263; SER-265; SER-282; SER-283; SER-287; SER-293; SER-297; SER-298 AND SER-299, IDENTIFICATION BY MASS SPECTROMETRY.
[8]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL136678 mRNA. Translation: CAB66613.1.
AK022490 mRNA. Translation: BAB14054.1.
AK023916 mRNA. Translation: BAB14723.1. Different initiation.
AK297822 mRNA. Translation: BAG60159.1.
AK315175 mRNA. Translation: BAG37616.1.
AC091563 Genomic DNA. No translation available.
AP005717 Genomic DNA. No translation available.
CH471060 Genomic DNA. Translation: EAW91997.1.
BC012040 mRNA. Translation: AAH12040.1.
BC024746 mRNA. Translation: AAH24746.1.
CCDSCCDS6331.1.
RefSeqNP_001269941.1. NM_001283012.1. [Q8TB45-2]
NP_073620.2. NM_022783.3. [Q8TB45-1]
UniGeneHs.112981.

3D structure databases

ProteinModelPortalQ8TB45.
SMRQ8TB45. Positions 32-121, 336-404.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid122304. 12 interactions.
IntActQ8TB45. 7 interactions.
MINTMINT-4712528.

PTM databases

PhosphoSiteQ8TB45.

Polymorphism databases

DMDM251757257.

Proteomic databases

MaxQBQ8TB45.
PaxDbQ8TB45.
PRIDEQ8TB45.

Protocols and materials databases

DNASU64798.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000286234; ENSP00000286234; ENSG00000155792.
ENST00000523492; ENSP00000430457; ENSG00000155792.
GeneID64798.
KEGGhsa:64798.
UCSCuc003yow.4. human. [Q8TB45-1]

Organism-specific databases

CTD64798.
GeneCardsGC08P120886.
H-InvDBHIX0017353.
HIX0025586.
HGNCHGNC:22953. DEPTOR.
HPACAB020841.
HPA023938.
HPA023945.
HPA024011.
MIM612974. gene.
neXtProtNX_Q8TB45.
PharmGKBPA134897957.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG125414.
HOVERGENHBG056008.
InParanoidQ8TB45.
OMARIYEKLM.
PhylomeDBQ8TB45.

Gene expression databases

ArrayExpressQ8TB45.
BgeeQ8TB45.
CleanExHS_DEPDC6.
GenevestigatorQ8TB45.

Family and domain databases

Gene3D1.10.10.10. 2 hits.
2.30.42.10. 1 hit.
InterProIPR000591. DEP_dom.
IPR001478. PDZ.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF00610. DEP. 2 hits.
[Graphical view]
SMARTSM00049. DEP. 2 hits.
SM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMSSF50156. SSF50156. 1 hit.
PROSITEPS50186. DEP. 2 hits.
PS50106. PDZ. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiDEPTOR.
GenomeRNAi64798.
NextBio35473550.
PROQ8TB45.
SOURCESearch...

Entry information

Entry nameDPTOR_HUMAN
AccessionPrimary (citable) accession number: Q8TB45
Secondary accession number(s): B2RCL9 expand/collapse secondary AC list , B4DN97, E7EV87, Q96EQ1, Q9H0R7, Q9H894, Q9HA07
Entry history
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: July 7, 2009
Last modified: July 9, 2014
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 8

Human chromosome 8: entries, gene names and cross-references to MIM