Q8TB40 (ABHD4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Abhydrolase domain-containing protein 4
EC=3.1.1.-

Alternative name(s):
Alpha/beta-hydrolase 4
Lyso-N-acylphosphatidylethanolamine lipase

Gene names

Name:

ABHD4

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Lysophospholipase selective for N-acyl phosphatidylethanolamine (NAPE). Contributes to the biosynthesis of N-acyl ethanolamines, including the endocannabinoid anandamide by hydrolyzing the sn-1 and sn-2 acyl chains from N-acyl phosphatidylethanolamine (NAPE) generating glycerophospho-N-acyl ethanolamine (GP-NAE), an intermediate for N-acyl ethanolamine biosynthesis. Hydrolyzes substrates bearing saturated, monounsaturated, polyunsaturated N-acyl chains. Shows no significant activity towards other lysophospholipids, including lysophosphatidylcholine, lysophosphatidylethanolamine and lysophosphatidylserine By similarity.
Sequence similarities	Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily.
Caution	Thr-291 is present instead of the conserved His which is expected to be an active site residue.

Ontologies

Keywords
Biological process	Lipid degradation Lipid metabolism
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 342	342	Abhydrolase domain-containing protein 4		PRO_0000080864
Experimental info
	Sequence conflict	183 – 185	3	EIR → GIC in BAB14289. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8TB40 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 867EA9E0092559CB	FASTA	342	38,794
	10 20 30 40 50 60 MADDLEQQSQ GWLSSWLPTW RPTSMSQLKN VEARILQCLQ NKFLARYVSL PNQNKIWTVT 70 80 90 100 110 120 VSPEQNDRTP LVMVHGFGGG VGLWILNMDS LSARRTLHTF DLLGFGRSSR PAFPRDPEGA 130 140 150 160 170 180 EDEFVTSIET WRETMGIPSM ILLGHSLGGF LATSYSIKYP DRVKHLILVD PWGFPLRPTN 190 200 210 220 230 240 PSEIRAPPAW VKAVASVLGR SNPLAVLRVA GPWGPGLVQR FRPDFKRKFA DFFEDDTISE 250 260 270 280 290 300 YIYHCNAQNP SGETAFKAMM ESFGWARRPM LERIHLIRKD VPITMIYGSD TWIDTSTGKK 310 320 330 340 VKMQRPDSYV RDMEIKGASH HVYADQPHIF NAVVEEICDS VD

References

[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Cervix.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK022878 mRNA. Translation: BAB14289.1. BC024779 mRNA. Translation: AAH24779.1.
IPI	IPI00290555.
RefSeq	NP_071343.2. NM_022060.2.
UniGene	Hs.445665.
3D structure databases
ProteinModelPortal	Q8TB40.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000216327.
Protein family/group databases
MEROPS	S33.013.
PTM databases
PhosphoSite	Q8TB40.
Polymorphism databases
DMDM	74762601.
Proteomic databases
PaxDb	Q8TB40.
PRIDE	Q8TB40.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000428304; ENSP00000414558; ENSG00000100439.
GeneID	63874.
KEGG	hsa:63874.
UCSC	uc001wgm.3. human.
Organism-specific databases
CTD	63874.
GeneCards	GC14P023067.
HGNC	HGNC:20154. ABHD4.
HPA	HPA000600.
neXtProt	NX_Q8TB40.
PharmGKB	PA128394705.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0596.
HOGENOM	HOG000007445.
HOVERGEN	HBG054445.
InParanoid	Q8TB40.
KO	K13698.
OMA	FTMADDL.
OrthoDB	EOG4NVZKQ.
PhylomeDB	Q8TB40.
Gene expression databases
ArrayExpress	Q8TB40.
Bgee	Q8TB40.
CleanEx	HS_ABHD4.
Genevestigator	Q8TB40.
GermOnline	ENSG00000100439. Homo sapiens.
Family and domain databases
InterPro	IPR000073. AB_hydrolase_1. [Graphical view]
PRINTS	PR00111. ABHYDROLASE.
ProtoNet	Search...
Other
ChiTaRS	ABHD4. human.
GenomeRNAi	63874.
NextBio	65552.

Entry information

Entry name

ABHD4_HUMAN

Accession

Primary (citable) accession number: Q8TB40
Secondary accession number(s): Q9H9E0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2005
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.