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Q8TB24 (RIN3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ras and Rab interactor 3
Alternative name(s):
Ras interaction/interference protein 3
Gene names
Name:RIN3
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length985 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Ras effector protein that functions as a guanine nucleotide exchange (GEF) for RAB5B and RAB31, by exchanging bound GDP for free GTP. Required for normal RAB31 function. Ref.1 Ref.7

Subunit structure

Interacts with CD2AP, RAB5B, RAB31 and BIN1. Ref.1 Ref.7 Ref.8

Subcellular location

Cytoplasm. Cytoplasmic vesicle. Early endosome. Note: Activation of tyrosine phosphorylation signaling induces translocation to cytoplasmic vesicles. Ref.1 Ref.6 Ref.7

Tissue specificity

Widely expressed. Ref.1

Sequence similarities

Belongs to the RIN (Ras interaction/interference) family.

Contains 1 Ras-associating domain.

Contains 1 SH2 domain.

Contains 1 VPS9 domain.

Sequence caution

The sequence BAB13888.1 differs from that shown. Reason: Aberrant splicing.

The sequence BAB15357.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence BAC03432.1 differs from that shown. Reason: Intron retention.

Binary interactions

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8TB24-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 4 (identifier: Q8TB24-4)

The sequence of this isoform differs from the canonical sequence as follows:
     878-903: DFICVSYLEPEQQARTLASRADTQAQ → VRPESGRGPVGPCPRHHPCCLLAKEP
     904-985: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 985985Ras and Rab interactor 3
PRO_0000191322

Regions

Domain63 – 15896SH2
Domain703 – 846144VPS9
Domain877 – 96387Ras-associating
Region587 – 732146Interaction with RAB5B
Compositional bias24 – 296Poly-Glu
Compositional bias248 – 514267Pro-rich
Compositional bias722 – 7254Poly-Thr
Compositional bias965 – 9728Poly-Gly

Natural variations

Alternative sequence878 – 90326DFICV…DTQAQ → VRPESGRGPVGPCPRHHPCC LLAKEP in isoform 4.
VSP_007587
Alternative sequence904 – 98582Missing in isoform 4.
VSP_007588
Natural variant1111E → K.
Corresponds to variant rs2274542 [ dbSNP | Ensembl ].
VAR_046645
Natural variant2151H → L.
Corresponds to variant rs3829947 [ dbSNP | Ensembl ].
VAR_059960
Natural variant2151H → P.
Corresponds to variant rs3829947 [ dbSNP | Ensembl ].
VAR_046646
Natural variant2151H → R.
Corresponds to variant rs3829947 [ dbSNP | Ensembl ].
VAR_059961
Natural variant4251T → I.
Corresponds to variant rs3742717 [ dbSNP | Ensembl ].
VAR_052946
Natural variant4251T → M. Ref.3
Corresponds to variant rs3742717 [ dbSNP | Ensembl ].
VAR_046647
Natural variant6131G → A.
Corresponds to variant rs12434929 [ dbSNP | Ensembl ].
VAR_046648

Experimental info

Mutagenesis8251Y → A: Strongly reduced guanine nucleotide exchange factor activity toward RAB31; when associated with A-828. Ref.7
Mutagenesis8281T → A: Strongly reduced guanine nucleotide exchange factor activity toward RAB31; when associated with A-825. Ref.7
Sequence conflict7421I → F in AAH25248. Ref.4
Sequence conflict9651Missing in BAB15357. Ref.3
Sequence conflict9651Missing in AAH25248. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 14, 2008. Version 4.
Checksum: A8949EA0D61570BB

FASTA985107,854
        10         20         30         40         50         60 
MIRHAGAPAR GDPTGPVPVV GKGEEEEEED GMRLCLPANP KNCLPHRRGI SILEKLIKTC 

        70         80         90        100        110        120 
PVWLQLSLGQ AEVARILHRV VAGMFLVRRD SSSKQLVLCV HFPSLNESSA EVLEYTIKEE 

       130        140        150        160        170        180 
KSILYLEGSA LVFEDIFRLI AFYCVSRDLL PFTLRLPQAI LEASSFTDLE TIANLGLGFW 

       190        200        210        220        230        240 
DSSLNPPQER GKPAEPPRDR APGFPLVSSL RPTAHDANCA CEIELSVGND RLWFVNPIFI 

       250        260        270        280        290        300 
EDCSSALPTD QPPLGNCPAR PLPPTSDATS PTSRWAPRRP PPPPPVLPLQ PCSPAQPPVL 

       310        320        330        340        350        360 
PALAPAPACP LPTSPPVPAP HVTPHAPGPP DHPNQPPMMT CERLPCPTAG LGPLREEAMK 

       370        380        390        400        410        420 
PGAASSPLQQ VPAPPLPAKK NLPTAPPRRR VSERVSLEDQ SPGMAAEGDQ LSLPPQGTSD 

       430        440        450        460        470        480 
GPEDTPREST EQGQDTEVKA SDPHSMPELP RTAKQPPVPP PRKKRISRQL ASTLPAPLEN 

       490        500        510        520        530        540 
AELCTQAMAL ETPTPGPPRE GQSPASQAGT QHPPAQATAH SQSSPEFKGS LASLSDSLGV 

       550        560        570        580        590        600 
SVMATDQDSY STSSTEEELE QFSSPSVKKK PSMILGKARH RLSFASFSSM FHAFLSNNRK 

       610        620        630        640        650        660 
LYKKVVELAQ DKGSYFGSLV QDYKVYSLEM MARQTSSTEM LQEIRTMMTQ LKSYLLQSTE 

       670        680        690        700        710        720 
LKALVDPALH SEEELEAIVE SALYKCVLKP LKEAINSCLH QIHSKDGSLQ QLKENQLVIL 

       730        740        750        760        770        780 
ATTTTDLGVT TSVPEVPMME KILQKFTSMH KAYSPEKKIS ILLKTCKLIY DSMALGNPGK 

       790        800        810        820        830        840 
PYGADDFLPV LMYVLARSNL TEMLLNVEYM MELMDPALQL GEGSYYLTTT YGALEHIKSY 

       850        860        870        880        890        900 
DKITVTRQLS VEVQDSIHRW ERRRTLNKAR ASRSSVQDFI CVSYLEPEQQ ARTLASRADT 

       910        920        930        940        950        960 
QAQALCAQCA EKFAVERPQA HRLFVLVDGR CFQLADDALP HCIKGYLLRS EPKRDFHFVY 

       970        980 
RPLDGGGGGG GGSPPCLVVR EPNFL 

« Hide

Isoform 4 [UniParc].

Checksum: 562A82ADCBD13127
Show »

FASTA90398,739

References

« Hide 'large scale' references
[1]"RIN3: a novel Rab5 GEF interacting with amphiphysin II involved in the early endocytic pathway."
Kajiho H., Saito K., Tsujita K., Kontani K., Araki Y., Kurosu H., Katada T.
J. Cell Sci. 116:4159-4168(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH BIN1 AND RAB5B, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]"The DNA sequence and analysis of human chromosome 14."
Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. expand/collapse author list , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.
Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-167 AND 405-985 (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 485-985 (ISOFORM 4), VARIANT MET-425.
Tissue: Embryo.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 328-985 (ISOFORM 1).
Tissue: Eye.
[5]"A novel binding protein composed of homophilic tetramer exhibits unique properties for the small GTPase Rab5."
Saito K., Murai J., Kajiho H., Kontani K., Kurosu H., Katada T.
J. Biol. Chem. 277:3412-3418(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 435-959 (ISOFORM 1).
Tissue: Leukocyte and Spleen.
[6]"Tyr-phosphorylation signals translocate RIN3, the small GTPase Rab5-GEF, to early endocytic vesicles."
Yoshikawa M., Kajiho H., Sakurai K., Minoda T., Nakagawa S., Kontani K., Katada T.
Biochem. Biophys. Res. Commun. 372:168-172(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, PHOSPHORYLATION.
[7]"Characterization of RIN3 as a guanine nucleotide exchange factor for the Rab5 subfamily GTPase Rab31."
Kajiho H., Sakurai K., Minoda T., Yoshikawa M., Nakagawa S., Fukushima S., Kontani K., Katada T.
J. Biol. Chem. 286:24364-24373(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF TYR-825 AND THR-828, INTERACTION WITH RAB31.
[8]"Atomic resolution crystal structure of the 2nd SH3 domain from human CD2AP (CMS) in complex with a proline-rich peptide from human RIN3."
Structural genomics consortium (SGC)
Submitted (DEC-2011) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (1.11 ANGSTROMS) OF 452-467 IN COMPLEX WITH CD2AP, INTERACTION WITH CD2AP.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB081753 mRNA. Translation: BAC16513.1.
AL136332 Genomic DNA. No translation available.
AL159141 Genomic DNA. No translation available.
AK021762 mRNA. Translation: BAB13888.1. Sequence problems.
AK026092 mRNA. Translation: BAB15357.1. Different initiation.
AK074176 mRNA. Translation: BAB85002.1.
AK090451 mRNA. Translation: BAC03432.1. Sequence problems.
BC025248 mRNA. Translation: AAH25248.2.
AB060338 mRNA. Translation: BAB84316.1.
PIRT50623.
RefSeqNP_079108.3. NM_024832.3.
UniGeneHs.326822.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3U23X-ray1.11B452-467[»]
ProteinModelPortalQ8TB24.
SMRQ8TB24. Positions 53-150, 636-846.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid122974. 7 interactions.
IntActQ8TB24. 13 interactions.
MINTMINT-273957.
STRING9606.ENSP00000216487.

PTM databases

PhosphoSiteQ8TB24.

Polymorphism databases

DMDM209572780.

Proteomic databases

PaxDbQ8TB24.
PRIDEQ8TB24.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000216487; ENSP00000216487; ENSG00000100599. [Q8TB24-1]
GeneID79890.
KEGGhsa:79890.
UCSCuc001yap.3. human. [Q8TB24-1]
uc001yas.1. human. [Q8TB24-4]

Organism-specific databases

CTD79890.
GeneCardsGC14P092980.
H-InvDBHIX0011904.
HGNCHGNC:18751. RIN3.
HPAHPA039836.
MIM610223. gene.
neXtProtNX_Q8TB24.
PharmGKBPA38673.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG295439.
HOVERGENHBG038998.
InParanoidQ8TB24.
OMAYTIKEEK.
OrthoDBEOG7PZRWP.
PhylomeDBQ8TB24.
TreeFamTF331067.

Gene expression databases

ArrayExpressQ8TB24.
BgeeQ8TB24.
CleanExHS_RIN3.
GenevestigatorQ8TB24.

Family and domain databases

Gene3D3.30.505.10. 1 hit.
InterProIPR000159. Ras-assoc.
IPR000980. SH2.
IPR003123. VPS9.
IPR013995. VPS9_subgr.
[Graphical view]
PfamPF02204. VPS9. 1 hit.
[Graphical view]
SMARTSM00314. RA. 1 hit.
SM00252. SH2. 1 hit.
SM00167. VPS9. 1 hit.
[Graphical view]
SUPFAMSSF55550. SSF55550. 1 hit.
PROSITEPS50200. RA. 1 hit.
PS50001. SH2. 1 hit.
PS51205. VPS9. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSRIN3. human.
GenomeRNAi79890.
NextBio69706.
PROQ8TB24.
SOURCESearch...

Entry information

Entry nameRIN3_HUMAN
AccessionPrimary (citable) accession number: Q8TB24
Secondary accession number(s): Q76LB3 expand/collapse secondary AC list , Q8NF30, Q8TEE8, Q8WYP4, Q9H6A5, Q9HAG1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 2003
Last sequence update: October 14, 2008
Last modified: April 16, 2014
This is version 107 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 14

Human chromosome 14: entries, gene names and cross-references to MIM