Q8TB24 (RIN3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ras and Rab interactor 3

Alternative name(s):
Ras interaction/interference protein 3

Gene names

Name:

RIN3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	985 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Ras effector protein that functions as a guanine nucleotide exchange (GEF) for RAB5B and RAB31, by exchanging bound GDP for free GTP. Required for normal RAB31 function. Ref.1 Ref.7
Subunit structure	Interacts with CD2AP, RAB5B, RAB31 and BIN1. Ref.1 Ref.7 Ref.8
Subcellular location	Cytoplasm. Cytoplasmic vesicle. Early endosome. Note: Activation of tyrosine phosphorylation signaling induces translocation to cytoplasmic vesicles. Ref.1 Ref.6 Ref.7
Tissue specificity	Widely expressed. Ref.1
Sequence similarities	Belongs to the RIN (Ras interaction/interference) family. Contains 1 Ras-associating domain. Contains 1 SH2 domain. Contains 1 VPS9 domain.
Sequence caution	The sequence BAB13888.1 differs from that shown. Reason: Aberrant splicing. The sequence BAB15357.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAC03432.1 differs from that shown. Reason: Intron retention.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoplasmic vesicle Endosome
Coding sequence diversity	Alternative splicing Polymorphism
Domain	SH2 domain
Molecular function	GTPase activation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	endocytosis Non-traceable author statement Ref.5. Source: UniProtKB positive regulation of GTPase activity Inferred from electronic annotation. Source: GOC signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasmic membrane-bounded vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasmic vesicle Inferred from direct assay Ref.7. Source: UniProtKB early endosome Inferred from direct assay Ref.7. Source: UniProtKB
Molecular_function	GTPase activator activity Inferred from electronic annotation. Source: UniProtKB-KW Rab GTPase binding Inferred from direct assay Ref.7. Source: UniProtKB Rab guanyl-nucleotide exchange factor activity Inferred from mutant phenotype Ref.7. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NCK1	P16333	2	EBI-1570523,EBI-389883
PLCG1	P19174	3	EBI-1570523,EBI-79387

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8TB24-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 4 (identifier: Q8TB24-4)

The sequence of this isoform differs from the canonical sequence as follows:
     878-903: DFICVSYLEPEQQARTLASRADTQAQ → VRPESGRGPVGPCPRHHPCCLLAKEP
     904-985: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 985	985	Ras and Rab interactor 3		PRO_0000191322
Regions
	Domain	63 – 158	96	SH2
	Domain	703 – 846	144	VPS9
	Domain	877 – 963	87	Ras-associating
	Region	587 – 732	146	Interaction with RAB5B
	Compositional bias	24 – 29	6	Poly-Glu
	Compositional bias	248 – 514	267	Pro-rich
	Compositional bias	722 – 725	4	Poly-Thr
	Compositional bias	965 – 972	8	Poly-Gly
Natural variations
	Alternative sequence	878 – 903	26	DFICV…DTQAQ → VRPESGRGPVGPCPRHHPCC LLAKEP in isoform 4.		VSP_007587
	Alternative sequence	904 – 985	82	Missing in isoform 4.		VSP_007588
	Natural variant	111	1	E → K. Corresponds to variant rs2274542 [ dbSNP | Ensembl ].		VAR_046645
	Natural variant	215	1	H → L. Corresponds to variant rs3829947 [ dbSNP | Ensembl ].		VAR_059960
	Natural variant	215	1	H → P. Corresponds to variant rs3829947 [ dbSNP | Ensembl ].		VAR_046646
	Natural variant	215	1	H → R. Corresponds to variant rs3829947 [ dbSNP | Ensembl ].		VAR_059961
	Natural variant	425	1	T → I. Corresponds to variant rs3742717 [ dbSNP | Ensembl ].		VAR_052946
	Natural variant	425	1	T → M. Ref.3 Corresponds to variant rs3742717 [ dbSNP | Ensembl ].		VAR_046647
	Natural variant	613	1	G → A. Corresponds to variant rs12434929 [ dbSNP | Ensembl ].		VAR_046648
Experimental info
	Mutagenesis	825	1	Y → A: Strongly reduced guanine nucleotide exchange factor activity toward RAB31; when associated with A-828. Ref.7
	Mutagenesis	828	1	T → A: Strongly reduced guanine nucleotide exchange factor activity toward RAB31; when associated with A-825. Ref.7
	Sequence conflict	742	1	I → F in AAH25248. Ref.4
	Sequence conflict	965	1	Missing in BAB15357. Ref.3
	Sequence conflict	965	1	Missing in AAH25248. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 14, 2008. Version 4. Checksum: A8949EA0D61570BB	FASTA	985	107,854
	10 20 30 40 50 60 MIRHAGAPAR GDPTGPVPVV GKGEEEEEED GMRLCLPANP KNCLPHRRGI SILEKLIKTC 70 80 90 100 110 120 PVWLQLSLGQ AEVARILHRV VAGMFLVRRD SSSKQLVLCV HFPSLNESSA EVLEYTIKEE 130 140 150 160 170 180 KSILYLEGSA LVFEDIFRLI AFYCVSRDLL PFTLRLPQAI LEASSFTDLE TIANLGLGFW 190 200 210 220 230 240 DSSLNPPQER GKPAEPPRDR APGFPLVSSL RPTAHDANCA CEIELSVGND RLWFVNPIFI 250 260 270 280 290 300 EDCSSALPTD QPPLGNCPAR PLPPTSDATS PTSRWAPRRP PPPPPVLPLQ PCSPAQPPVL 310 320 330 340 350 360 PALAPAPACP LPTSPPVPAP HVTPHAPGPP DHPNQPPMMT CERLPCPTAG LGPLREEAMK 370 380 390 400 410 420 PGAASSPLQQ VPAPPLPAKK NLPTAPPRRR VSERVSLEDQ SPGMAAEGDQ LSLPPQGTSD 430 440 450 460 470 480 GPEDTPREST EQGQDTEVKA SDPHSMPELP RTAKQPPVPP PRKKRISRQL ASTLPAPLEN 490 500 510 520 530 540 AELCTQAMAL ETPTPGPPRE GQSPASQAGT QHPPAQATAH SQSSPEFKGS LASLSDSLGV 550 560 570 580 590 600 SVMATDQDSY STSSTEEELE QFSSPSVKKK PSMILGKARH RLSFASFSSM FHAFLSNNRK 610 620 630 640 650 660 LYKKVVELAQ DKGSYFGSLV QDYKVYSLEM MARQTSSTEM LQEIRTMMTQ LKSYLLQSTE 670 680 690 700 710 720 LKALVDPALH SEEELEAIVE SALYKCVLKP LKEAINSCLH QIHSKDGSLQ QLKENQLVIL 730 740 750 760 770 780 ATTTTDLGVT TSVPEVPMME KILQKFTSMH KAYSPEKKIS ILLKTCKLIY DSMALGNPGK 790 800 810 820 830 840 PYGADDFLPV LMYVLARSNL TEMLLNVEYM MELMDPALQL GEGSYYLTTT YGALEHIKSY 850 860 870 880 890 900 DKITVTRQLS VEVQDSIHRW ERRRTLNKAR ASRSSVQDFI CVSYLEPEQQ ARTLASRADT 910 920 930 940 950 960 QAQALCAQCA EKFAVERPQA HRLFVLVDGR CFQLADDALP HCIKGYLLRS EPKRDFHFVY 970 980 RPLDGGGGGG GGSPPCLVVR EPNFL
	Isoform 4 [UniParc]. Checksum: 562A82ADCBD13127	FASTA	903	98,739
	10 20 30 40 50 60 MIRHAGAPAR GDPTGPVPVV GKGEEEEEED GMRLCLPANP KNCLPHRRGI SILEKLIKTC 70 80 90 100 110 120 PVWLQLSLGQ AEVARILHRV VAGMFLVRRD SSSKQLVLCV HFPSLNESSA EVLEYTIKEE 130 140 150 160 170 180 KSILYLEGSA LVFEDIFRLI AFYCVSRDLL PFTLRLPQAI LEASSFTDLE TIANLGLGFW 190 200 210 220 230 240 DSSLNPPQER GKPAEPPRDR APGFPLVSSL RPTAHDANCA CEIELSVGND RLWFVNPIFI 250 260 270 280 290 300 EDCSSALPTD QPPLGNCPAR PLPPTSDATS PTSRWAPRRP PPPPPVLPLQ PCSPAQPPVL 310 320 330 340 350 360 PALAPAPACP LPTSPPVPAP HVTPHAPGPP DHPNQPPMMT CERLPCPTAG LGPLREEAMK 370 380 390 400 410 420 PGAASSPLQQ VPAPPLPAKK NLPTAPPRRR VSERVSLEDQ SPGMAAEGDQ LSLPPQGTSD 430 440 450 460 470 480 GPEDTPREST EQGQDTEVKA SDPHSMPELP RTAKQPPVPP PRKKRISRQL ASTLPAPLEN 490 500 510 520 530 540 AELCTQAMAL ETPTPGPPRE GQSPASQAGT QHPPAQATAH SQSSPEFKGS LASLSDSLGV 550 560 570 580 590 600 SVMATDQDSY STSSTEEELE QFSSPSVKKK PSMILGKARH RLSFASFSSM FHAFLSNNRK 610 620 630 640 650 660 LYKKVVELAQ DKGSYFGSLV QDYKVYSLEM MARQTSSTEM LQEIRTMMTQ LKSYLLQSTE 670 680 690 700 710 720 LKALVDPALH SEEELEAIVE SALYKCVLKP LKEAINSCLH QIHSKDGSLQ QLKENQLVIL 730 740 750 760 770 780 ATTTTDLGVT TSVPEVPMME KILQKFTSMH KAYSPEKKIS ILLKTCKLIY DSMALGNPGK 790 800 810 820 830 840 PYGADDFLPV LMYVLARSNL TEMLLNVEYM MELMDPALQL GEGSYYLTTT YGALEHIKSY 850 860 870 880 890 900 DKITVTRQLS VEVQDSIHRW ERRRTLNKAR ASRSSVQVRP ESGRGPVGPC PRHHPCCLLA KEP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"RIN3: a novel Rab5 GEF interacting with amphiphysin II involved in the early endocytic pathway." Kajiho H., Saito K., Tsujita K., Kontani K., Araki Y., Kurosu H., Katada T. J. Cell Sci. 116:4159-4168(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH BIN1 AND RAB5B, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]	"The DNA sequence and analysis of human chromosome 14." Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J. Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-167 AND 405-985 (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 485-985 (ISOFORM 4), VARIANT MET-425. Tissue: Embryo.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 328-985 (ISOFORM 1). Tissue: Eye.
[5]	"A novel binding protein composed of homophilic tetramer exhibits unique properties for the small GTPase Rab5." Saito K., Murai J., Kajiho H., Kontani K., Kurosu H., Katada T. J. Biol. Chem. 277:3412-3418(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 435-959 (ISOFORM 1). Tissue: Leukocyte and Spleen.
[6]	"Tyr-phosphorylation signals translocate RIN3, the small GTPase Rab5-GEF, to early endocytic vesicles." Yoshikawa M., Kajiho H., Sakurai K., Minoda T., Nakagawa S., Kontani K., Katada T. Biochem. Biophys. Res. Commun. 372:168-172(2008) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, PHOSPHORYLATION.
[7]	"Characterization of RIN3 as a guanine nucleotide exchange factor for the Rab5 subfamily GTPase Rab31." Kajiho H., Sakurai K., Minoda T., Yoshikawa M., Nakagawa S., Fukushima S., Kontani K., Katada T. J. Biol. Chem. 286:24364-24373(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF TYR-825 AND THR-828, INTERACTION WITH RAB31.
[8]	"Atomic resolution crystal structure of the 2nd SH3 domain from human CD2AP (CMS) in complex with a proline-rich peptide from human RIN3." Structural genomics consortium (SGC) Submitted (DEC-2011) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.11 ANGSTROMS) OF 452-467 IN COMPLEX WITH CD2AP, INTERACTION WITH CD2AP.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB081753 mRNA. Translation: BAC16513.1.
AL136332 Genomic DNA. No translation available.
AL159141 Genomic DNA. No translation available.
AK021762 mRNA. Translation: BAB13888.1. Sequence problems.
AK026092 mRNA. Translation: BAB15357.1. Different initiation.
AK074176 mRNA. Translation: BAB85002.1.
AK090451 mRNA. Translation: BAC03432.1. Sequence problems.
BC025248 mRNA. Translation: AAH25248.2.
AB060338 mRNA. Translation: BAB84316.1.

IPI

IPI00329749.
IPI00745485.

PIR

T50623.

RefSeq

NP_079108.3. NM_024832.3.

UniGene

Hs.326822.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3U23	X-ray	1.11	B	452-467	[»]

ProteinModelPortal

Q8TB24.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q8TB24. 11 interactions.

MINT

MINT-273957.

STRING

9606.ENSP00000216487.

PTM databases

PhosphoSite

Q8TB24.

Polymorphism databases

DMDM

209572780.

Proteomic databases

PaxDb

Q8TB24.

PRIDE

Q8TB24.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000216487; ENSP00000216487; ENSG00000100599.

GeneID

79890.

KEGG

hsa:79890.

UCSC

uc001yap.3. human.
uc001yas.1. human.

Organism-specific databases

CTD

79890.

GeneCards

GC14P092980.

H-InvDB

HIX0011904.

HGNC

HGNC:18751. RIN3.

HPA

HPA039836.

MIM

610223. gene.

neXtProt

NX_Q8TB24.

PharmGKB

PA38673.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG295439.

HOVERGEN

HBG038998.

InParanoid

Q8TB24.

OMA

RLWFVNP.

OrthoDB

EOG45QHCW.

PhylomeDB

Q8TB24.

Gene expression databases

ArrayExpress

Q8TB24.

Bgee

Q8TB24.

CleanEx

HS_RIN3.

Genevestigator

Q8TB24.

GermOnline

ENSG00000100599. Homo sapiens.

Family and domain databases

Gene3D

3.30.505.10. 1 hit.

InterPro

IPR000159. Ras-assoc.
IPR000980. SH2.
IPR003123. VPS9.
IPR013995. VPS9_subgr.
[Graphical view]

Pfam

PF02204. VPS9. 1 hit.
[Graphical view]

SMART

SM00314. RA. 1 hit.
SM00252. SH2. 1 hit.
SM00167. VPS9. 1 hit.
[Graphical view]

PROSITE

PS50200. RA. 1 hit.
PS50001. SH2. 1 hit.
PS51205. VPS9. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

RIN3. human.

GenomeRNAi

79890.

NextBio

69706.

SOURCE

Search...

Entry information

Entry name

RIN3_HUMAN

Accession

Primary (citable) accession number: Q8TB24
Secondary accession number(s): Q76LB3 , Q8NF30, Q8TEE8, Q8WYP4, Q9H6A5, Q9HAG1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2003
Last sequence update:	October 14, 2008
Last modified:	May 1, 2013
This is version 99 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.