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Protein

Cytochrome P450 2W1

Gene

CYP2W1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Seems to have broad catalytic activity towards several chemicals, including polycyclic aromatic hydrocarbon dihydrodiols and aromatic amines (PubMed:16551781, PubMed:24278521). Actives also in the metabolism of indoline substrates and is able to activate aflatoxin B1 into cytotoxic products (PubMed:20805301). Furthermore, it seems to be involved in the oxydation of lysophospholipids and fatty acids (PubMed:22591743).4 Publications

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi433 – 4331Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. heme binding Source: GO_Central
  2. iron ion binding Source: InterPro
  3. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen Source: GO_Central
  4. oxygen binding Source: GO_Central
  5. steroid hydroxylase activity Source: GO_Central

GO - Biological processi

  1. aflatoxin B1 metabolic process Source: UniProtKB
  2. exogenous drug catabolic process Source: GO_Central
  3. organic acid metabolic process Source: GO_Central
  4. oxidation-reduction process Source: GO_Central
  5. small molecule metabolic process Source: Reactome
  6. xenobiotic metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_13425. Miscellaneous substrates.
REACT_13543. Xenobiotics.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2W1 (EC:1.14.14.-)
Alternative name(s):
CYPIIW1
Gene namesi
Name:CYP2W1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:20243. CYP2W1.

Subcellular locationi

Endoplasmic reticulum lumen 1 Publication. Cell membrane 1 Publication
Note: About 8% are expressed on the cell surface.1 Publication

GO - Cellular componenti

  1. cell surface Source: UniProtKB
  2. cytoplasm Source: GO_Central
  3. endoplasmic reticulum lumen Source: UniProtKB
  4. endoplasmic reticulum membrane Source: Reactome
  5. intracellular membrane-bounded organelle Source: GO_Central
  6. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi177 – 1771N → A: Loss of glycosylation. 1 Publication

Organism-specific databases

PharmGKBiPA134992665.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Chaini23 – 490468Cytochrome P450 2W1Sequence AnalysisPRO_0000051782Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi177 – 1771N-linked (GlcNAc...)1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ8TAV3.
PaxDbiQ8TAV3.
PRIDEiQ8TAV3.

Expressioni

Tissue specificityi

Very low levels are detected in fetal and adult tissues. Highly expressed in several tumor samples, in particular colon and adrenal tumors.1 Publication

Gene expression databases

BgeeiQ8TAV3.
CleanExiHS_CYP2W1.
ExpressionAtlasiQ8TAV3. baseline and differential.
GenevestigatoriQ8TAV3.

Organism-specific databases

HPAiHPA012753.

Interactioni

Protein-protein interaction databases

BioGridi120251. 4 interactions.
IntActiQ8TAV3. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ8TAV3.
SMRiQ8TAV3. Positions 58-489.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118775.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiQ8TAV3.
KOiK07423.
OMAiPARAFTM.
OrthoDBiEOG7RBZ85.
PhylomeDBiQ8TAV3.
TreeFamiTF352043.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8TAV3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALLLLLFLG LLGLWGLLCA CAQDPSPAAR WPPGPRPLPL VGNLHLLRLS
60 70 80 90 100
QQDRSLMELS ERYGPVFTVH LGRQKTVVLT GFEAVKEALA GPGQELADRP
110 120 130 140 150
PIAIFQLIQR GGGIFFSSGA RWRAARQFTV RALHSLGVGR EPVADKILQE
160 170 180 190 200
LKCLSGQLDG YRGRPFPLAL LGWAPSNITF ALLFGRRFDY RDPVFVSLLG
210 220 230 240 250
LIDEVMVLLG SPGLQLFNVY PWLGALLQLH RPVLRKIEEV RAILRTLLEA
260 270 280 290 300
RRPHVCPGDP VCSYVDALIQ QGQGDDPEGL FAEANAVACT LDMVMAGTET
310 320 330 340 350
TSATLQWAAL LMGRHPDVQG RVQEELDRVL GPGRTPRLED QQALPYTSAV
360 370 380 390 400
LHEVQRFITL LPHVPRCTAA DTQLGGFLLP KGTPVIPLLT SVLLDETQWQ
410 420 430 440 450
TPGQFNPGHF LDANGHFVKR EAFLPFSAGR RVCVGERLAR TELFLLFAGL
460 470 480 490
LQRYRLLPPP GVSPASLDTT PARAFTMRPR AQALCAVPRP
Length:490
Mass (Da):53,844
Last modified:September 5, 2006 - v2
Checksum:iBB84B2F1FEFEF5BC
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti58 – 581E → A.1 Publication
VAR_071912
Natural varianti181 – 1811A → T Associated with increased colorectal cancer risk. 1 Publication
Corresponds to variant rs3735684 [ dbSNP | Ensembl ].
VAR_027413
Natural varianti432 – 4321V → I.1 Publication
VAR_071913
Natural varianti482 – 4821Q → H.1 Publication
VAR_071914
Natural varianti488 – 4881P → L.1 Publication
VAR_071915

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC073957 Genomic DNA. No translation available.
BC025761 mRNA. Translation: AAH25761.2.
CCDSiCCDS5319.2.
RefSeqiNP_060251.2. NM_017781.2.
XP_005249849.1. XM_005249792.1.
UniGeneiHs.272795.

Genome annotation databases

EnsembliENST00000308919; ENSP00000310149; ENSG00000073067.
GeneIDi54905.
KEGGihsa:54905.
UCSCiuc003sjq.1. human.

Polymorphism databases

DMDMi114152790.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC073957 Genomic DNA. No translation available.
BC025761 mRNA. Translation: AAH25761.2.
CCDSiCCDS5319.2.
RefSeqiNP_060251.2. NM_017781.2.
XP_005249849.1. XM_005249792.1.
UniGeneiHs.272795.

3D structure databases

ProteinModelPortaliQ8TAV3.
SMRiQ8TAV3. Positions 58-489.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120251. 4 interactions.
IntActiQ8TAV3. 2 interactions.

Chemistry

ChEMBLiCHEMBL2406897.

Polymorphism databases

DMDMi114152790.

Proteomic databases

MaxQBiQ8TAV3.
PaxDbiQ8TAV3.
PRIDEiQ8TAV3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000308919; ENSP00000310149; ENSG00000073067.
GeneIDi54905.
KEGGihsa:54905.
UCSCiuc003sjq.1. human.

Organism-specific databases

CTDi54905.
GeneCardsiGC07P001022.
HGNCiHGNC:20243. CYP2W1.
HPAiHPA012753.
MIMi615967. gene.
neXtProtiNX_Q8TAV3.
PharmGKBiPA134992665.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118775.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiQ8TAV3.
KOiK07423.
OMAiPARAFTM.
OrthoDBiEOG7RBZ85.
PhylomeDBiQ8TAV3.
TreeFamiTF352043.

Enzyme and pathway databases

ReactomeiREACT_13425. Miscellaneous substrates.
REACT_13543. Xenobiotics.

Miscellaneous databases

GeneWikiiCYP2W1.
GenomeRNAii54905.
NextBioi57946.
PROiQ8TAV3.
SOURCEiSearch...

Gene expression databases

BgeeiQ8TAV3.
CleanExiHS_CYP2W1.
ExpressionAtlasiQ8TAV3. baseline and differential.
GenevestigatoriQ8TAV3.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  2. "The DNA sequence of human chromosome 7."
    Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L.
    , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
    Nature 424:157-164(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: TISSUE SPECIFICITY.
  4. "Recombinant enzymes overexpressed in bacteria show broad catalytic specificity of human cytochrome P450 2W1 and limited activity of human cytochrome P450 2S1."
    Wu Z.L., Sohl C.D., Shimada T., Guengerich F.P.
    Mol. Pharmacol. 69:2007-2014(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Colorectal cancer-specific cytochrome P450 2W1: intracellular localization, glycosylation, and catalytic activity."
    Gomez A., Nekvindova J., Travica S., Lee M.Y., Johansson I., Edler D., Mkrtchian S., Ingelman-Sundberg M.
    Mol. Pharmacol. 78:1004-1011(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, GLYCOSYLATION AT ASN-177, MUTAGENESIS OF ASN-177, FUNCTION, IDENTIFICATION OF SUBSTRAT.
  6. "Bioactivation of aromatic amines by human CYP2W1, an orphan cytochrome P450 enzyme."
    Eun C.Y., Han S., Lim Y.R., Park H.G., Han J.S., Cho K.S., Chun Y.J., Kim D.
    Toxicol. Res. 26:171-175(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION OF SUBSTRAT.
  7. "Metabolomic analysis and identification of a role for the orphan human cytochrome P450 2W1 in selective oxidation of lysophospholipids."
    Xiao Y., Guengerich F.P.
    J. Lipid Res. 53:1610-1617(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION OF SUBSTRAT.
  8. "CYP2W1 variant alleles in Caucasians and association of the CYP2W1 G541A (Ala181Thr) polymorphism with increased colorectal cancer risk."
    Gervasini G., de Murillo S.G., Ladero J.M., Agundez J.A.
    Pharmacogenomics 11:919-925(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS ALA-58; THR-181; ILE-432; HIS-482 AND LEU-488.

Entry informationi

Entry nameiCP2W1_HUMAN
AccessioniPrimary (citable) accession number: Q8TAV3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: September 5, 2006
Last modified: April 1, 2015
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.