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Protein

Zinc finger protein 417

Gene

ZNF417

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in transcriptional regulation.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri92 – 11423C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri240 – 26223C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri268 – 29023C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri296 – 31823C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri324 – 34623C2H2-type 5; degeneratePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri352 – 37423C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri380 – 40223C2H2-type 7PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri408 – 43023C2H2-type 8PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri436 – 45823C2H2-type 9PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri464 – 48623C2H2-type 10PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri492 – 51423C2H2-type 11PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri520 – 54223C2H2-type 12PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri548 – 57023C2H2-type 13PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_12627. Generic Transcription Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 417
Gene namesi
Name:ZNF417
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:20646. ZNF417.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134863699.

Polymorphism and mutation databases

BioMutaiZNF417.
DMDMi296453053.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 575575Zinc finger protein 417PRO_0000233985Add
BLAST

Proteomic databases

MaxQBiQ8TAU3.
PaxDbiQ8TAU3.
PRIDEiQ8TAU3.

PTM databases

PhosphoSiteiQ8TAU3.

Expressioni

Gene expression databases

BgeeiQ8TAU3.
CleanExiHS_ZNF417.
ExpressionAtlasiQ8TAU3. baseline.
GenevisibleiQ8TAU3. HS.

Organism-specific databases

HPAiHPA042125.
HPA043288.
HPA056213.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ADAMTSL4Q6UY14-33EBI-740727,EBI-10173507
BEND5Q7L4P63EBI-740727,EBI-724373
CARD9Q9H2573EBI-740727,EBI-751319
CCDC136Q96JN2-23EBI-740727,EBI-10171416
CCDC57Q2TAC23EBI-740727,EBI-2808286
CEP44Q9C0F14EBI-740727,EBI-744115
CEP70Q8NHQ13EBI-740727,EBI-739624
CSRNP1Q96S653EBI-740727,EBI-4311573
EFSO432813EBI-740727,EBI-718488
FHL2Q141923EBI-740727,EBI-701903
FHL3Q136433EBI-740727,EBI-741101
FSD2A1L4K13EBI-740727,EBI-5661036
GOLGA2Q083793EBI-740727,EBI-618309
HMBOX1Q6NT763EBI-740727,EBI-2549423
IKZF1Q134223EBI-740727,EBI-745305
KLC3Q6P5973EBI-740727,EBI-1643885
KRT15P190123EBI-740727,EBI-739566
KRT38O760153EBI-740727,EBI-1047263
KRT40Q6A1623EBI-740727,EBI-10171697
KRTAP10-5P603705EBI-740727,EBI-10172150
KRTAP10-7P604093EBI-740727,EBI-10172290
KRTAP10-8P604103EBI-740727,EBI-10171774
KRTAP10-9P604113EBI-740727,EBI-10172052
KRTAP4-2Q9BYR53EBI-740727,EBI-10172511
KRTAP5-9P263713EBI-740727,EBI-3958099
KXD1Q9BQD33EBI-740727,EBI-739657
LURAP1Q96LR23EBI-740727,EBI-741355
LZTS2Q9BRK43EBI-740727,EBI-741037
MDFIQ997504EBI-740727,EBI-724076
MEOX2A4D1273EBI-740727,EBI-10172134
MID2Q9UJV3-23EBI-740727,EBI-10172526
MTUS2Q5JR593EBI-740727,EBI-742948
NDEL1Q9GZM83EBI-740727,EBI-928842
NINLQ9Y2I64EBI-740727,EBI-719716
NOTCH2NLQ7Z3S93EBI-740727,EBI-945833
PHC2Q8IXK03EBI-740727,EBI-713786
PLSCR1O151623EBI-740727,EBI-740019
PNMA2Q9UL424EBI-740727,EBI-302355
RELQ048643EBI-740727,EBI-307352
RPGRIP1Q96KN73EBI-740727,EBI-1050213
RUNDC3AQ59EK93EBI-740727,EBI-747225
SPRY2O435973EBI-740727,EBI-742487
SSX2IPQ9Y2D83EBI-740727,EBI-2212028
STX11O755583EBI-740727,EBI-714135
TCF4P158843EBI-740727,EBI-533224
TENC1Q63HR23EBI-740727,EBI-949753
TFIP11Q9UBB94EBI-740727,EBI-1105213
TMCC2Q7Z6C63EBI-740727,EBI-10177480
TRAF1Q130773EBI-740727,EBI-359224
TRIM27P143734EBI-740727,EBI-719493
TRIM36Q9NQ863EBI-740727,EBI-2341518
TRIM37O949724EBI-740727,EBI-741602
TRIM41Q8WV443EBI-740727,EBI-725997
TRIM54Q9BYV23EBI-740727,EBI-2130429
TSGA10Q9BZW73EBI-740727,EBI-744794
ZBTB1Q9Y2K13EBI-740727,EBI-2682961
ZBTB10Q96DT73EBI-740727,EBI-10235384
ZBTB8AQ96BR93EBI-740727,EBI-742740
ZNF587Q96SQ53EBI-740727,EBI-6427977

Protein-protein interaction databases

BioGridi127075. 66 interactions.
IntActiQ8TAU3. 66 interactions.
MINTiMINT-1483592.
STRINGi9606.ENSP00000311319.

Structurei

3D structure databases

ProteinModelPortaliQ8TAU3.
SMRiQ8TAU3. Positions 106-573.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini15 – 8874KRABPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 13 C2H2-type zinc fingers.PROSITE-ProRule annotation
Contains 1 KRAB domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri92 – 11423C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri240 – 26223C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri268 – 29023C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri296 – 31823C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri324 – 34623C2H2-type 5; degeneratePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri352 – 37423C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri380 – 40223C2H2-type 7PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri408 – 43023C2H2-type 8PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri436 – 45823C2H2-type 9PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri464 – 48623C2H2-type 10PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri492 – 51423C2H2-type 11PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri520 – 54223C2H2-type 12PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri548 – 57023C2H2-type 13PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5048.
GeneTreeiENSGT00760000119107.
HOGENOMiHOG000234617.
HOVERGENiHBG018163.
InParanoidiQ8TAU3.
KOiK09228.
OMAiDHPETHH.
OrthoDBiEOG7KSX7Q.
PhylomeDBiQ8TAU3.
TreeFamiTF339848.

Family and domain databases

Gene3Di3.30.160.60. 13 hits.
InterProiIPR001909. Krueppel-associated_box.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF01352. KRAB. 1 hit.
PF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTiSM00349. KRAB. 1 hit.
SM00355. ZnF_C2H2. 13 hits.
[Graphical view]
SUPFAMiSSF109640. SSF109640. 1 hit.
PROSITEiPS50805. KRAB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 12 hits.
PS50157. ZINC_FINGER_C2H2_2. 13 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8TAU3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAAPRRPT QQGTVTFEDV AVNFSQEEWC LLSEAQRCLY RDVMLENLAL
60 70 80 90 100
ISSLGCWCGS KDEEAPCKQR ISVQRESQSR TPRAGVSPKK AHPCEMCGLI
110 120 130 140 150
LEDVFHFADH QETHHKQKLN RSGACGKNLD DTAYLHQHQK QHIGEKFYRK
160 170 180 190 200
SVREASFVKK RKLRVSQEPF VFREFGKDVL PSSGLCQEAA AVEKTDSETM
210 220 230 240 250
HGPPFQEGKT NYSCGKRTKA FSTKHSVIPH QKLFTRDGCY VCSDCGKSFS
260 270 280 290 300
RYVSFSNHQR DHTAKGPYDC GECGKSYSRK SSLIQHQRVH TGKTAYPCEE
310 320 330 340 350
CGKSFSQKGS LISHQRVHTG ERPYECREYG KSFGQKGNLI QHQQGHTGER
360 370 380 390 400
AYHCGECGKS FRQKFCFINH QRVHTGERPY KCGECGKSFG QKGNLVQHQR
410 420 430 440 450
GHTGERPYEC KECGKSFRYR SHLTEHQRLH TGERPYNCRE CGKLFNRKYH
460 470 480 490 500
LLVHERVHTG ERPYACEVCG KLFGNKNCVT IHQRIHTGER PYECNECGKS
510 520 530 540 550
FLSSSALHVH KRVHSGQKPY KCSECGKSFA ECSSLIKHRR IHTGERPYEC
560 570
TKCGKTFQRS STLLHHQSSH RRKAL
Length:575
Mass (Da):65,733
Last modified:May 18, 2010 - v2
Checksum:i8CE2FB25CBE474F5
GO
Isoform 2 (identifier: Q8TAU3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-199: Missing.

Note: No experimental confirmation available.
Show »
Length:376
Mass (Da):43,212
Checksum:i9E787AC7E84A2898
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti173 – 1731R → H in AAH25783 (PubMed:15489334).Curated
Sequence conflicti316 – 3161R → L in AAH25783 (PubMed:15489334).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti495 – 4951N → S.1 Publication
Corresponds to variant rs10416584 [ dbSNP | Ensembl ].
VAR_060275

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 199199Missing in isoform 2. 1 PublicationVSP_055950Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK293789 mRNA. Translation: BAG57202.1.
AC010326 Genomic DNA. No translation available.
BC025783 mRNA. Translation: AAH25783.1.
CCDSiCCDS12965.1. [Q8TAU3-1]
RefSeqiNP_689688.2. NM_152475.2. [Q8TAU3-1]
UniGeneiHs.567710.

Genome annotation databases

EnsembliENST00000312026; ENSP00000311319; ENSG00000173480. [Q8TAU3-1]
GeneIDi147687.
KEGGihsa:147687.
UCSCiuc002qqq.3. human. [Q8TAU3-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK293789 mRNA. Translation: BAG57202.1.
AC010326 Genomic DNA. No translation available.
BC025783 mRNA. Translation: AAH25783.1.
CCDSiCCDS12965.1. [Q8TAU3-1]
RefSeqiNP_689688.2. NM_152475.2. [Q8TAU3-1]
UniGeneiHs.567710.

3D structure databases

ProteinModelPortaliQ8TAU3.
SMRiQ8TAU3. Positions 106-573.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi127075. 66 interactions.
IntActiQ8TAU3. 66 interactions.
MINTiMINT-1483592.
STRINGi9606.ENSP00000311319.

PTM databases

PhosphoSiteiQ8TAU3.

Polymorphism and mutation databases

BioMutaiZNF417.
DMDMi296453053.

Proteomic databases

MaxQBiQ8TAU3.
PaxDbiQ8TAU3.
PRIDEiQ8TAU3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000312026; ENSP00000311319; ENSG00000173480. [Q8TAU3-1]
GeneIDi147687.
KEGGihsa:147687.
UCSCiuc002qqq.3. human. [Q8TAU3-1]

Organism-specific databases

CTDi147687.
GeneCardsiGC19M058417.
HGNCiHGNC:20646. ZNF417.
HPAiHPA042125.
HPA043288.
HPA056213.
neXtProtiNX_Q8TAU3.
PharmGKBiPA134863699.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5048.
GeneTreeiENSGT00760000119107.
HOGENOMiHOG000234617.
HOVERGENiHBG018163.
InParanoidiQ8TAU3.
KOiK09228.
OMAiDHPETHH.
OrthoDBiEOG7KSX7Q.
PhylomeDBiQ8TAU3.
TreeFamiTF339848.

Enzyme and pathway databases

ReactomeiREACT_12627. Generic Transcription Pathway.

Miscellaneous databases

GenomeRNAii147687.
NextBioi35471316.
PROiQ8TAU3.

Gene expression databases

BgeeiQ8TAU3.
CleanExiHS_ZNF417.
ExpressionAtlasiQ8TAU3. baseline.
GenevisibleiQ8TAU3. HS.

Family and domain databases

Gene3Di3.30.160.60. 13 hits.
InterProiIPR001909. Krueppel-associated_box.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF01352. KRAB. 1 hit.
PF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTiSM00349. KRAB. 1 hit.
SM00355. ZnF_C2H2. 13 hits.
[Graphical view]
SUPFAMiSSF109640. SSF109640. 1 hit.
PROSITEiPS50805. KRAB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 12 hits.
PS50157. ZINC_FINGER_C2H2_2. 13 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Cerebellum.
  2. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT SER-495.
    Tissue: Pancreas.

Entry informationi

Entry nameiZN417_HUMAN
AccessioniPrimary (citable) accession number: Q8TAU3
Secondary accession number(s): B4DEU1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: May 18, 2010
Last modified: June 24, 2015
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.